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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 39 |
| AasI | GACNNNNNNGTC | 1 cut(s) | 222 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 101, 102 |
| AgsI | TTSAA | 2 cut(s) | 77, 89 |
| AluBI | AGCT | 1 cut(s) | 154 |
| AluI | AGCT | 1 cut(s) | 154 |
| Alw26I | GTCTC | 1 cut(s) | 13 |
| AoxI | GGCC | 1 cut(s) | 91 |
| AspS9I | GGNCC | 2 cut(s) | 92, 247 |
| AvaII | GGWCC | 1 cut(s) | 247 |
| BcoDI | GTCTC | 1 cut(s) | 13 |
| BfaI | CTAG | 1 cut(s) | 234 |
| BfmI | CTRYAG | 1 cut(s) | 51 |
| Bme18I | GGWCC | 1 cut(s) | 247 |
| BmgT120I | GGNCC | 2 cut(s) | 92, 247 |
| BmiI | GGNNCC | 3 cut(s) | 94, 248, 249 |
| BpuEI | CTTGAG | 1 cut(s) | 152 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 101, 102 |
| Bse3DI | GCAATG | 1 cut(s) | 70 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 101, 102 |
| BseMI | GCAATG | 1 cut(s) | 70 |
| BshFI | GGCC | 1 cut(s) | 93 |
| BslFI | GGGAC | 1 cut(s) | 260 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 101, 102 |
| BsmAI | GTCTC | 1 cut(s) | 13 |
| BsmFI | GGGAC | 1 cut(s) | 260 |
| BsnI | GGCC | 1 cut(s) | 93 |
| BspANI | GGCC | 1 cut(s) | 93 |
| BspLI | GGNNCC | 3 cut(s) | 94, 248, 249 |
| BsrDI | GCAATG | 1 cut(s) | 70 |
| Bst4CI | ACNGT | 1 cut(s) | 52 |
| Bst6I | CTCTTC | 1 cut(s) | 51 |
| BstMAI | GTCTC | 1 cut(s) | 13 |
| BstSFI | CTRYAG | 1 cut(s) | 51 |
| BsuRI | GGCC | 1 cut(s) | 93 |
| Cfr13I | GGNCC | 2 cut(s) | 92, 247 |
| CseI | GACGC | 1 cut(s) | 212 |
| CviAII | CATG | 1 cut(s) | 82 |
| CviJI | RGCY | 4 cut(s) | 93, 154, 201, 213 |
| CviKI_1 | RGCY | 4 cut(s) | 93, 154, 201, 213 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 222 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 222 |
| Eam1104I | CTCTTC | 1 cut(s) | 51 |
| EarI | CTCTTC | 1 cut(s) | 51 |
| Eco47I | GGWCC | 1 cut(s) | 247 |
| EcoO109I | RGGNCCY | 2 cut(s) | 92, 247 |
| FaeI | CATG | 1 cut(s) | 85 |
| FaiI | YATR | 6 cut(s) | 39, 83, 186, 191, 221, 228 |
| FaqI | GGGAC | 1 cut(s) | 260 |
| FatI | CATG | 1 cut(s) | 81 |
| FspBI | CTAG | 1 cut(s) | 234 |
| HaeIII | GGCC | 1 cut(s) | 93 |
| HgaI | GACGC | 1 cut(s) | 212 |
| Hin1II | CATG | 1 cut(s) | 85 |
| HinfI | GANTC | 1 cut(s) | 6 |
| Hpy166II | GTNNAC | 1 cut(s) | 26 |
| Hpy8I | GTNNAC | 1 cut(s) | 26 |
| HpyAV | CCTTC | 1 cut(s) | 83 |
| HpyCH4III | ACNGT | 1 cut(s) | 52 |
| Hsp92II | CATG | 1 cut(s) | 85 |
| KflI | GGGWCCC | 1 cut(s) | 247 |
| MaeI | CTAG | 1 cut(s) | 234 |
| MboII | GAAGA | 1 cut(s) | 68 |
| MlyI | GAGTC | 1 cut(s) | 15 |
| MnlI | CCTC | 1 cut(s) | 139 |
| MseI | TTAA | 1 cut(s) | 117 |
| NlaIII | CATG | 1 cut(s) | 85 |
| NlaIV | GGNNCC | 3 cut(s) | 94, 248, 249 |
| PleI | GAGTC | 1 cut(s) | 14 |
| PpsI | GAGTC | 1 cut(s) | 14 |
| PpuMI | RGGWCCY | 1 cut(s) | 247 |
| PsiI | TTATAA | 1 cut(s) | 39 |
| Psp5II | RGGWCCY | 1 cut(s) | 247 |
| PspN4I | GGNNCC | 3 cut(s) | 94, 248, 249 |
| PspPI | GGNCC | 2 cut(s) | 92, 247 |
| PspPPI | RGGWCCY | 1 cut(s) | 247 |
| SaqAI | TTAA | 1 cut(s) | 117 |
| Sau96I | GGNCC | 2 cut(s) | 92, 247 |
| SchI | GAGTC | 1 cut(s) | 15 |
| SetI | ASST | 2 cut(s) | 156, 239 |
| SfcI | CTRYAG | 1 cut(s) | 51 |
| SgeI | CNNG | 9 cut(s) | 26, 57, 94, 113, 122, 181, 191, 246, 262 |
| SinI | GGWCC | 1 cut(s) | 247 |
| SmlI | CTYRAG | 1 cut(s) | 167 |
| SmoI | CTYRAG | 1 cut(s) | 167 |
| SspMI | CTAG | 1 cut(s) | 234 |
| TaaI | ACNGT | 1 cut(s) | 52 |
| Tru1I | TTAA | 1 cut(s) | 117 |
| Tru9I | TTAA | 1 cut(s) | 117 |
| VpaK11BI | GGWCC | 1 cut(s) | 247 |
| XspI | CTAG | 1 cut(s) | 234 |