source UniProtKB
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 954 |
| Acc65I | GGTACC | 1 cut(s) | 654 |
| AccB1I | GGYRCC | 2 cut(s) | 489, 654 |
| AccI | GTMKAC | 1 cut(s) | 334 |
| AccII | CGCG | 2 cut(s) | 729, 804 |
| AciI | CCGC | 1 cut(s) | 371 |
| AcoI | YGGCCR | 1 cut(s) | 1288 |
| AcsI | RAATTY | 1 cut(s) | 353 |
| AcuI | CTGAAG | 1 cut(s) | 1060 |
| AfaI | GTAC | 4 cut(s) | 377, 429, 439, 656 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 452, 470, 512, 519, 589, 651, 860, 861, 1156 |
| AflIII | ACRYGT | 1 cut(s) | 996 |
| AgeI | ACCGGT | 1 cut(s) | 265 |
| AgsI | TTSAA | 7 cut(s) | 118, 223, 248, 650, 755, 941, 1165 |
| AhlI | ACTAGT | 1 cut(s) | 1214 |
| AjnI | CCWGG | 3 cut(s) | 413, 433, 1285 |
| AleI | CACNNNNGTG | 2 cut(s) | 1215, 1225 |
| AluBI | AGCT | 9 cut(s) | 29, 144, 746, 1045, 1070, 1125, 1146, 1250, 1262 |
| AluI | AGCT | 9 cut(s) | 29, 144, 746, 1045, 1070, 1125, 1146, 1250, 1262 |
| Alw21I | GWGCWC | 2 cut(s) | 504, 1222 |
| Alw26I | GTCTC | 3 cut(s) | 248, 395, 903 |
| Alw44I | GTGCAC | 1 cut(s) | 1218 |
| Ama87I | CYCGRG | 2 cut(s) | 497, 512 |
| AoxI | GGCC | 6 cut(s) | 417, 450, 521, 830, 990, 1288 |
| ApaI | GGGCCC | 1 cut(s) | 421 |
| ApaLI | GTGCAC | 1 cut(s) | 1218 |
| ApeKI | GCWGC | 5 cut(s) | 29, 141, 230, 288, 1122 |
| ApoI | RAATTY | 1 cut(s) | 353 |
| AsiGI | ACCGGT | 1 cut(s) | 265 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 366 |
| Asp718I | GGTACC | 1 cut(s) | 654 |
| AspLEI | GCGC | 2 cut(s) | 729, 885 |
| AspS9I | GGNCC | 4 cut(s) | 417, 418, 450, 990 |
| AsuC2I | CCSGG | 5 cut(s) | 151, 454, 513, 514, 659 |
| AsuHPI | GGTGA | 4 cut(s) | 87, 191, 458, 1009 |
| AvaI | CYCGRG | 2 cut(s) | 497, 512 |
| BaeGI | GKGCMC | 2 cut(s) | 421, 1222 |
| BalI | TGGCCA | 1 cut(s) | 1290 |
| BanI | GGYRCC | 2 cut(s) | 489, 654 |
| BanII | GRGCYC | 3 cut(s) | 421, 480, 513 |
| BbsI | GAAGAC | 1 cut(s) | 606 |
| Bbv12I | GWGCWC | 2 cut(s) | 504, 1222 |
| BbvI | GCAGC | 5 cut(s) | 16, 153, 217, 300, 1134 |
| BccI | CCATC | 6 cut(s) | 430, 542, 614, 1004, 1028, 1062 |
| BciT130I | CCWGG | 3 cut(s) | 415, 435, 1287 |
| BclI | TGATCA | 1 cut(s) | 925 |
| BcnI | CCSGG | 5 cut(s) | 151, 454, 513, 514, 659 |
| BcoDI | GTCTC | 3 cut(s) | 248, 395, 903 |
| BcuI | ACTAGT | 1 cut(s) | 1214 |
| BfaI | CTAG | 6 cut(s) | 195, 383, 408, 1215, 1259, 1301 |
| BfuAI | ACCTGC | 1 cut(s) | 954 |
| BisI | GCNGC | 5 cut(s) | 30, 142, 231, 289, 1123 |
| BlsI | GCNGC | 5 cut(s) | 31, 143, 232, 290, 1124 |
| Bme1390I | CCNGG | 8 cut(s) | 151, 415, 435, 454, 513, 514, 659, 1287 |
| BmeT110I | CYCGRG | 2 cut(s) | 497, 512 |
| BmgT120I | GGNCC | 4 cut(s) | 417, 418, 450, 990 |
| BmiI | GGNNCC | 5 cut(s) | 419, 420, 479, 491, 656 |
| BmrFI | CCNGG | 8 cut(s) | 151, 415, 435, 454, 513, 514, 659, 1287 |
| BmsI | GCATC | 5 cut(s) | 308, 572, 748, 796, 1167 |
| BpiI | GAAGAC | 1 cut(s) | 606 |
| BpuEI | CTTGAG | 1 cut(s) | 638 |
| BpuMI | CCSGG | 5 cut(s) | 151, 454, 513, 514, 659 |
| BsaBI | GATNNNNATC | 1 cut(s) | 924 |
| BsaJI | CCNNGG | 9 cut(s) | 18, 413, 414, 433, 446, 469, 512, 513, 697 |
| BsaWI | WCCGGW | 2 cut(s) | 265, 1266 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 29, 59 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 452, 470, 512, 519, 589, 651, 860, 861, 1156 |
| Bse118I | RCCGGY | 1 cut(s) | 265 |
| Bse3DI | GCAATG | 1 cut(s) | 12 |
| Bse8I | GATNNNNATC | 1 cut(s) | 924 |
| BseBI | CCWGG | 3 cut(s) | 415, 435, 1287 |
| BseDI | CCNNGG | 9 cut(s) | 18, 413, 414, 433, 446, 469, 512, 513, 697 |
| BseGI | GGATG | 8 cut(s) | 88, 247, 319, 606, 1015, 1020, 1054, 1090 |
| BseJI | GATNNNNATC | 1 cut(s) | 924 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 452, 470, 512, 519, 589, 651, 860, 861, 1156 |
| BseMI | GCAATG | 1 cut(s) | 12 |
| BseRI | GAGGAG | 1 cut(s) | 266 |
| BseSI | GKGCMC | 2 cut(s) | 421, 1222 |
| BseXI | GCAGC | 5 cut(s) | 16, 153, 217, 300, 1134 |
| BsgI | GTGCAG | 1 cut(s) | 216 |
| Bsh1236I | CGCG | 2 cut(s) | 729, 804 |
| BshFI | GGCC | 6 cut(s) | 419, 452, 523, 832, 992, 1290 |
| BshNI | GGYRCC | 2 cut(s) | 489, 654 |
| BshTI | ACCGGT | 1 cut(s) | 265 |
| BsiHKAI | GWGCWC | 2 cut(s) | 504, 1222 |
| BsiHKCI | CYCGRG | 2 cut(s) | 497, 512 |
| BsiSI | CCGG | 6 cut(s) | 151, 266, 453, 513, 659, 1267 |
| BslFI | GGGAC | 1 cut(s) | 565 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 452, 470, 512, 519, 589, 651, 860, 861, 1156 |
| BsmAI | GTCTC | 3 cut(s) | 248, 395, 903 |
| BsmFI | GGGAC | 1 cut(s) | 565 |
| BsnI | GGCC | 6 cut(s) | 419, 452, 523, 832, 992, 1290 |
| BsoBI | CYCGRG | 2 cut(s) | 497, 512 |
| Bsp120I | GGGCCC | 1 cut(s) | 417 |
| Bsp1286I | GDGCHC | 5 cut(s) | 421, 480, 504, 513, 1222 |
| Bsp1407I | TGTACA | 1 cut(s) | 375 |
| Bsp143I | GATC | 4 cut(s) | 187, 628, 925, 1188 |
| Bsp19I | CCATGG | 3 cut(s) | 446, 469, 697 |
| BspACI | CCGC | 1 cut(s) | 371 |
| BspANI | GGCC | 6 cut(s) | 419, 452, 523, 832, 992, 1290 |
| BspFNI | CGCG | 2 cut(s) | 729, 804 |
| BspHI | TCATGA | 1 cut(s) | 928 |
| BspLI | GGNNCC | 5 cut(s) | 419, 420, 479, 491, 656 |
| BspMI | ACCTGC | 1 cut(s) | 954 |
| BspQI | GCTCTTC | 1 cut(s) | 1035 |
| BspT107I | GGYRCC | 2 cut(s) | 489, 654 |
| BsrDI | GCAATG | 1 cut(s) | 12 |
| BsrFI | RCCGGY | 1 cut(s) | 265 |
| BsrGI | TGTACA | 1 cut(s) | 375 |
| BssAI | RCCGGY | 1 cut(s) | 265 |
| BssECI | CCNNGG | 9 cut(s) | 18, 413, 414, 433, 446, 469, 512, 513, 697 |
| BssMI | GATC | 4 cut(s) | 187, 628, 925, 1188 |
| BssT1I | CCWWGG | 4 cut(s) | 18, 446, 469, 697 |
| Bst2UI | CCWGG | 3 cut(s) | 415, 435, 1287 |
| Bst4CI | ACNGT | 2 cut(s) | 202, 859 |
| Bst6I | CTCTTC | 3 cut(s) | 243, 893, 1035 |
| BstAUI | TGTACA | 1 cut(s) | 375 |
| BstC8I | GCNNGC | 3 cut(s) | 525, 873, 951 |
| BstDEI | CTNAG | 1 cut(s) | 1315 |
| BstDSI | CCRYGG | 3 cut(s) | 446, 469, 697 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 1154 |
| BstF5I | GGATG | 8 cut(s) | 88, 247, 319, 606, 1015, 1020, 1054, 1090 |
| BstFNI | CGCG | 2 cut(s) | 729, 804 |
| BstHHI | GCGC | 2 cut(s) | 729, 885 |
| BstKTI | GATC | 4 cut(s) | 190, 631, 928, 1191 |
| BstMAI | GTCTC | 3 cut(s) | 248, 395, 903 |
| BstMBI | GATC | 4 cut(s) | 187, 628, 925, 1188 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 508, 524 |
| BstNI | CCWGG | 3 cut(s) | 415, 435, 1287 |
| BstNSI | RCATGY | 1 cut(s) | 822 |
| BstSCI | CCNGG | 8 cut(s) | 149, 413, 433, 452, 511, 512, 657, 1285 |
| BstSLI | GKGCMC | 2 cut(s) | 421, 1222 |
| BstUI | CGCG | 2 cut(s) | 729, 804 |
| BstV1I | GCAGC | 5 cut(s) | 16, 153, 217, 300, 1134 |
| BstV2I | GAAGAC | 1 cut(s) | 606 |
| BsuRI | GGCC | 6 cut(s) | 419, 452, 523, 832, 992, 1290 |
| BtgI | CCRYGG | 3 cut(s) | 446, 469, 697 |
| BtgZI | GCGATG | 1 cut(s) | 819 |
| BtsCI | GGATG | 8 cut(s) | 88, 247, 319, 606, 1015, 1020, 1054, 1090 |
| BveI | ACCTGC | 1 cut(s) | 954 |
| Cac8I | GCNNGC | 3 cut(s) | 525, 873, 951 |
| CciI | TCATGA | 1 cut(s) | 928 |
| CfoI | GCGC | 2 cut(s) | 729, 885 |
| Cfr10I | RCCGGY | 1 cut(s) | 265 |
| Cfr13I | GGNCC | 4 cut(s) | 417, 418, 450, 990 |
| Cfr9I | CCCGGG | 1 cut(s) | 512 |
| Csp6I | GTAC | 4 cut(s) | 376, 428, 438, 655 |
| CspAI | ACCGGT | 1 cut(s) | 265 |
| CviAII | CATG | 8 cut(s) | 447, 470, 632, 698, 819, 929, 1096, 1105 |
| CviQI | GTAC | 4 cut(s) | 376, 428, 438, 655 |
| DdeI | CTNAG | 1 cut(s) | 1315 |
| DpnI | GATC | 4 cut(s) | 189, 630, 927, 1190 |
| DpnII | GATC | 4 cut(s) | 187, 628, 925, 1188 |
| EaeI | YGGCCR | 1 cut(s) | 1288 |
| Eam1104I | CTCTTC | 3 cut(s) | 243, 893, 1035 |
| EarI | CTCTTC | 3 cut(s) | 243, 893, 1035 |
| Eco130I | CCWWGG | 4 cut(s) | 18, 446, 469, 697 |
| Eco24I | GRGCYC | 3 cut(s) | 421, 480, 513 |
| Eco32I | GATATC | 1 cut(s) | 1116 |
| Eco57I | CTGAAG | 1 cut(s) | 1060 |
| Eco88I | CYCGRG | 2 cut(s) | 497, 512 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 1154 |
| EcoO109I | RGGNCCY | 1 cut(s) | 418 |
| EcoRII | CCWGG | 3 cut(s) | 413, 433, 1285 |
| EcoRV | GATATC | 1 cut(s) | 1116 |
| EcoT14I | CCWWGG | 4 cut(s) | 18, 446, 469, 697 |
| EcoT38I | GRGCYC | 3 cut(s) | 421, 480, 513 |
| ErhI | CCWWGG | 4 cut(s) | 18, 446, 469, 697 |
| FaeI | CATG | 8 cut(s) | 450, 473, 635, 701, 822, 932, 1099, 1108 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 16, 48, 203, 235 |
| FaqI | GGGAC | 1 cut(s) | 565 |
| FatI | CATG | 8 cut(s) | 446, 469, 631, 697, 818, 928, 1095, 1104 |
| FbaI | TGATCA | 1 cut(s) | 925 |
| FblI | GTMKAC | 1 cut(s) | 334 |
| Fnu4HI | GCNGC | 5 cut(s) | 30, 142, 231, 289, 1123 |
| FokI | GGATG | 8 cut(s) | 95, 254, 326, 593, 1007, 1022, 1041, 1097 |
| FriOI | GRGCYC | 3 cut(s) | 421, 480, 513 |
| Fsp4HI | GCNGC | 5 cut(s) | 30, 142, 231, 289, 1123 |
| FspBI | CTAG | 6 cut(s) | 195, 383, 408, 1215, 1259, 1301 |
| GlaI | GCGC | 2 cut(s) | 728, 884 |
| GluI | GCNGC | 5 cut(s) | 30, 142, 231, 289, 1123 |
| HaeIII | GGCC | 6 cut(s) | 419, 452, 523, 832, 992, 1290 |
| HapII | CCGG | 6 cut(s) | 151, 266, 453, 513, 659, 1267 |
| HhaI | GCGC | 2 cut(s) | 729, 885 |
| Hin1II | CATG | 8 cut(s) | 450, 473, 635, 701, 822, 932, 1099, 1108 |
| Hin6I | GCGC | 2 cut(s) | 727, 883 |
| HinP1I | GCGC | 2 cut(s) | 727, 883 |
| HincII | GTYRAC | 1 cut(s) | 205 |
| HindII | GTYRAC | 1 cut(s) | 205 |
| HinfI | GANTC | 3 cut(s) | 92, 388, 602 |
| HpaII | CCGG | 6 cut(s) | 151, 266, 453, 513, 659, 1267 |
| HphI | GGTGA | 4 cut(s) | 87, 191, 458, 1009 |
| Hpy166II | GTNNAC | 5 cut(s) | 205, 335, 459, 1017, 1220 |
| Hpy188I | TCNGA | 1 cut(s) | 568 |
| Hpy188III | TCNNGA | 5 cut(s) | 132, 220, 346, 929, 1078 |
| Hpy8I | GTNNAC | 5 cut(s) | 205, 335, 459, 1017, 1220 |
| HpyAV | CCTTC | 4 cut(s) | 166, 947, 1102, 1204 |
| HpyCH4III | ACNGT | 2 cut(s) | 202, 859 |
| HpyCH4IV | ACGT | 1 cut(s) | 998 |
| HpyCH4V | TGCA | 3 cut(s) | 233, 299, 1220 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 508, 524 |
| HpyF3I | CTNAG | 1 cut(s) | 1315 |
| HpySE526I | ACGT | 1 cut(s) | 998 |
| Hsp92II | CATG | 8 cut(s) | 450, 473, 635, 701, 822, 932, 1099, 1108 |
| HspAI | GCGC | 2 cut(s) | 727, 883 |
| KpnI | GGTACC | 1 cut(s) | 658 |
| Ksp22I | TGATCA | 1 cut(s) | 925 |
| Kzo9I | GATC | 4 cut(s) | 187, 628, 925, 1188 |
| LguI | GCTCTTC | 1 cut(s) | 1035 |
| LmnI | GCTCC | 1 cut(s) | 483 |
| Lsp1109I | GCAGC | 5 cut(s) | 16, 153, 217, 300, 1134 |
| LweI | GCATC | 5 cut(s) | 308, 572, 748, 796, 1167 |
| MaeI | CTAG | 6 cut(s) | 195, 383, 408, 1215, 1259, 1301 |
| MaeII | ACGT | 1 cut(s) | 998 |
| MaeIII | GTNAC | 1 cut(s) | 736 |
| MalI | GATC | 4 cut(s) | 189, 630, 927, 1190 |
| MboI | GATC | 4 cut(s) | 187, 628, 925, 1188 |
| MhlI | GDGCHC | 5 cut(s) | 421, 480, 504, 513, 1222 |
| MlsI | TGGCCA | 1 cut(s) | 1290 |
| MluCI | AATT | 4 cut(s) | 281, 353, 546, 888 |
| MluNI | TGGCCA | 1 cut(s) | 1290 |
| MlyI | GAGTC | 2 cut(s) | 397, 596 |
| MmeI | TCCRAC | 1 cut(s) | 36 |
| Mox20I | TGGCCA | 1 cut(s) | 1290 |
| MroXI | GAANNNNTTC | 1 cut(s) | 366 |
| MscI | TGGCCA | 1 cut(s) | 1290 |
| MseI | TTAA | 2 cut(s) | 215, 1004 |
| MslI | CAYNNNNRTG | 2 cut(s) | 1215, 1225 |
| Msp20I | TGGCCA | 1 cut(s) | 1290 |
| MspA1I | CMGCKG | 2 cut(s) | 144, 373 |
| MspI | CCGG | 6 cut(s) | 151, 266, 453, 513, 659, 1267 |
| MspR9I | CCNGG | 8 cut(s) | 151, 415, 435, 454, 513, 514, 659, 1287 |
| MvaI | CCWGG | 3 cut(s) | 415, 435, 1287 |
| MvnI | CGCG | 2 cut(s) | 729, 804 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 508, 524 |
| NciI | CCSGG | 5 cut(s) | 151, 454, 513, 514, 659 |
| NcoI | CCATGG | 3 cut(s) | 446, 469, 697 |
| NdeII | GATC | 4 cut(s) | 187, 628, 925, 1188 |
| NlaIII | CATG | 8 cut(s) | 450, 473, 635, 701, 822, 932, 1099, 1108 |
| NlaIV | GGNNCC | 5 cut(s) | 419, 420, 479, 491, 656 |
| NspI | RCATGY | 1 cut(s) | 822 |
| OliI | CACNNNNGTG | 2 cut(s) | 1215, 1225 |
| PagI | TCATGA | 1 cut(s) | 928 |
| PasI | CCCWGGG | 1 cut(s) | 414 |
| PciSI | GCTCTTC | 1 cut(s) | 1035 |
| PdmI | GAANNNNTTC | 1 cut(s) | 366 |
| PfeI | GAWTC | 1 cut(s) | 92 |
| PinAI | ACCGGT | 1 cut(s) | 265 |
| PkrI | GCNGC | 5 cut(s) | 31, 143, 232, 290, 1124 |
| PleI | GAGTC | 2 cut(s) | 396, 596 |
| PpsI | GAGTC | 2 cut(s) | 396, 596 |
| Psp6I | CCWGG | 3 cut(s) | 413, 433, 1285 |
| PspGI | CCWGG | 3 cut(s) | 413, 433, 1285 |
| PspN4I | GGNNCC | 5 cut(s) | 419, 420, 479, 491, 656 |
| PspOMI | GGGCCC | 1 cut(s) | 417 |
| PspPI | GGNCC | 4 cut(s) | 417, 418, 450, 990 |
| PvuII | CAGCTG | 1 cut(s) | 144 |
| RsaI | GTAC | 4 cut(s) | 377, 429, 439, 656 |
| RsaNI | GTAC | 4 cut(s) | 376, 428, 438, 655 |
| RseI | CAYNNNNRTG | 2 cut(s) | 1215, 1225 |
| SapI | GCTCTTC | 1 cut(s) | 1035 |
| SaqAI | TTAA | 2 cut(s) | 215, 1004 |
| SatI | GCNGC | 5 cut(s) | 30, 142, 231, 289, 1123 |
| Sau3AI | GATC | 4 cut(s) | 187, 628, 925, 1188 |
| Sau96I | GGNCC | 4 cut(s) | 417, 418, 450, 990 |
| SchI | GAGTC | 2 cut(s) | 397, 596 |
| ScrFI | CCNGG | 8 cut(s) | 151, 415, 435, 454, 513, 514, 659, 1287 |
| SduI | GDGCHC | 5 cut(s) | 421, 480, 504, 513, 1222 |
| SfaNI | GCATC | 5 cut(s) | 308, 572, 748, 796, 1167 |
| SmaI | CCCGGG | 1 cut(s) | 514 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 1215, 1225 |
| SmlI | CTYRAG | 1 cut(s) | 617 |
| SmoI | CTYRAG | 1 cut(s) | 617 |
| SpeI | ACTAGT | 1 cut(s) | 1214 |
| Sse9I | AATT | 4 cut(s) | 281, 353, 546, 888 |
| SsiI | CCGC | 1 cut(s) | 371 |
| SspMI | CTAG | 6 cut(s) | 195, 383, 408, 1215, 1259, 1301 |
| StyD4I | CCNGG | 8 cut(s) | 149, 413, 433, 452, 511, 512, 657, 1285 |
| StyI | CCWWGG | 4 cut(s) | 18, 446, 469, 697 |
| TaaI | ACNGT | 2 cut(s) | 202, 859 |
| TaiI | ACGT | 1 cut(s) | 1001 |
| TaqI | TCGA | 2 cut(s) | 588, 1118 |
| TasI | AATT | 4 cut(s) | 281, 353, 546, 888 |
| TatI | WGTACW | 1 cut(s) | 375 |
| TfiI | GAWTC | 1 cut(s) | 92 |
| Tru1I | TTAA | 2 cut(s) | 215, 1004 |
| Tru9I | TTAA | 2 cut(s) | 215, 1004 |
| TseI | GCWGC | 5 cut(s) | 29, 141, 230, 288, 1122 |
| TspDTI | ATGAA | 3 cut(s) | 185, 1121, 1186 |
| TspMI | CCCGGG | 1 cut(s) | 512 |
| VneI | GTGCAC | 1 cut(s) | 1218 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 1154 |
| XapI | RAATTY | 1 cut(s) | 353 |
| XceI | RCATGY | 1 cut(s) | 822 |
| XmaI | CCCGGG | 1 cut(s) | 512 |
| XmiI | GTMKAC | 1 cut(s) | 334 |
| XmnI | GAANNNNTTC | 1 cut(s) | 366 |
| XspI | CTAG | 6 cut(s) | 195, 383, 408, 1215, 1259, 1301 |