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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 1 cut(s) | 121 |
| AcsI | RAATTY | 1 cut(s) | 94 |
| AgsI | TTSAA | 1 cut(s) | 157 |
| AjiI | CACGTC | 1 cut(s) | 11 |
| AlwI | GGATC | 1 cut(s) | 121 |
| ApoI | RAATTY | 1 cut(s) | 94 |
| BlpI | GCTNAGC | 1 cut(s) | 144 |
| BmgBI | CACGTC | 1 cut(s) | 11 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 144 |
| Bsp143I | GATC | 1 cut(s) | 126 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 144 |
| BspPI | GGATC | 1 cut(s) | 121 |
| BssMI | GATC | 1 cut(s) | 126 |
| BstDEI | CTNAG | 1 cut(s) | 144 |
| BstKTI | GATC | 1 cut(s) | 129 |
| BstMBI | GATC | 1 cut(s) | 126 |
| BstX2I | RGATCY | 1 cut(s) | 126 |
| BstYI | RGATCY | 1 cut(s) | 126 |
| BtrI | CACGTC | 1 cut(s) | 11 |
| CviAII | CATG | 1 cut(s) | 34 |
| CviJI | RGCY | 1 cut(s) | 143 |
| CviKI_1 | RGCY | 1 cut(s) | 143 |
| DdeI | CTNAG | 1 cut(s) | 144 |
| DpnI | GATC | 1 cut(s) | 128 |
| DpnII | GATC | 1 cut(s) | 126 |
| FaeI | CATG | 1 cut(s) | 37 |
| FaiI | YATR | 3 cut(s) | 26, 35, 150 |
| FatI | CATG | 1 cut(s) | 33 |
| Hin1II | CATG | 1 cut(s) | 37 |
| Hpy166II | GTNNAC | 1 cut(s) | 103 |
| Hpy188I | TCNGA | 1 cut(s) | 131 |
| Hpy8I | GTNNAC | 1 cut(s) | 103 |
| HpyCH4IV | ACGT | 1 cut(s) | 10 |
| HpyF3I | CTNAG | 1 cut(s) | 144 |
| HpySE526I | ACGT | 1 cut(s) | 10 |
| Hsp92II | CATG | 1 cut(s) | 37 |
| Kzo9I | GATC | 1 cut(s) | 126 |
| MaeII | ACGT | 1 cut(s) | 10 |
| MaeIII | GTNAC | 1 cut(s) | 29 |
| MalI | GATC | 1 cut(s) | 128 |
| MboI | GATC | 1 cut(s) | 126 |
| MflI | RGATCY | 1 cut(s) | 126 |
| MluCI | AATT | 3 cut(s) | 42, 94, 169 |
| MnlI | CCTC | 2 cut(s) | 43, 81 |
| MseI | TTAA | 2 cut(s) | 75, 172 |
| NdeII | GATC | 1 cut(s) | 126 |
| NlaIII | CATG | 1 cut(s) | 37 |
| PsuI | RGATCY | 1 cut(s) | 126 |
| SaqAI | TTAA | 2 cut(s) | 75, 172 |
| Sau3AI | GATC | 1 cut(s) | 126 |
| SetI | ASST | 2 cut(s) | 13, 92 |
| SgeI | CNNG | 8 cut(s) | 18, 23, 46, 67, 73, 99, 124, 143 |
| Sse9I | AATT | 3 cut(s) | 42, 94, 169 |
| TaiI | ACGT | 1 cut(s) | 13 |
| TasI | AATT | 3 cut(s) | 42, 94, 169 |
| Tru1I | TTAA | 2 cut(s) | 75, 172 |
| Tru9I | TTAA | 2 cut(s) | 75, 172 |
| TspDTI | ATGAA | 2 cut(s) | 10, 50 |
| XapI | RAATTY | 1 cut(s) | 94 |