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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 2 cut(s) | 2181, 2698 |
| AatII | GACGTC | 1 cut(s) | 552 |
| AccB1I | GGYRCC | 2 cut(s) | 2329, 2415 |
| AccI | GTMKAC | 2 cut(s) | 1698, 2226 |
| AccII | CGCG | 2 cut(s) | 1758, 2274 |
| AciI | CCGC | 5 cut(s) | 397, 999, 2032, 2148, 2256 |
| AclI | AACGTT | 1 cut(s) | 770 |
| AclWI | GGATC | 5 cut(s) | 30, 514, 2396, 2409, 2573 |
| AcoI | YGGCCR | 1 cut(s) | 1859 |
| AcsI | RAATTY | 4 cut(s) | 722, 880, 2070, 2602 |
| AcuI | CTGAAG | 2 cut(s) | 392, 1226 |
| AcyI | GRCGYC | 2 cut(s) | 549, 2416 |
| AfaI | GTAC | 3 cut(s) | 514, 810, 1774 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 1056, 1548, 1902 |
| AflII | CTTAAG | 1 cut(s) | 1495 |
| AflIII | ACRYGT | 4 cut(s) | 1052, 1591, 2130, 2691 |
| AgeI | ACCGGT | 3 cut(s) | 353, 1384, 2240 |
| AgsI | TTSAA | 9 cut(s) | 265, 445, 664, 976, 1013, 1037, 1153, 2191, 2300 |
| AjnI | CCWGG | 2 cut(s) | 1322, 2311 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2429, 2461 |
| AleI | CACNNNNGTG | 1 cut(s) | 2241 |
| Alw21I | GWGCWC | 2 cut(s) | 2248, 2478 |
| Alw26I | GTCTC | 4 cut(s) | 540, 989, 1181, 1700 |
| Alw44I | GTGCAC | 2 cut(s) | 2244, 2474 |
| AlwI | GGATC | 5 cut(s) | 30, 514, 2396, 2409, 2573 |
| AlwNI | CAGNNNCTG | 2 cut(s) | 29, 1994 |
| Ama87I | CYCGRG | 1 cut(s) | 1094 |
| AoxI | GGCC | 3 cut(s) | 321, 1859, 2016 |
| ApaLI | GTGCAC | 2 cut(s) | 2244, 2474 |
| ApeKI | GCWGC | 8 cut(s) | 62, 164, 641, 1376, 1714, 1886, 2151, 2253 |
| ApoI | RAATTY | 4 cut(s) | 722, 880, 2070, 2602 |
| AseI | ATTAAT | 1 cut(s) | 2501 |
| AsiGI | ACCGGT | 3 cut(s) | 353, 1384, 2240 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 199 |
| AspLEI | GCGC | 3 cut(s) | 1758, 2276, 2418 |
| AspS9I | GGNCC | 1 cut(s) | 2016 |
| AsuHPI | GGTGA | 6 cut(s) | 640, 845, 1039, 1442, 2035, 2441 |
| AvaI | CYCGRG | 1 cut(s) | 1094 |
| BaeGI | GKGCMC | 2 cut(s) | 2248, 2478 |
| BamHI | GGATCC | 1 cut(s) | 2401 |
| BanI | GGYRCC | 2 cut(s) | 2329, 2415 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1779, 1811 |
| BbsI | GAAGAC | 1 cut(s) | 2601 |
| Bbv12I | GWGCWC | 2 cut(s) | 2248, 2478 |
| BbvCI | CCTCAGC | 2 cut(s) | 984, 1290 |
| BbvI | GCAGC | 8 cut(s) | 74, 151, 628, 1363, 1726, 1898, 2138, 2240 |
| BccI | CCATC | 6 cut(s) | 376, 1147, 1337, 1465, 2030, 2054 |
| BceAI | ACGGC | 2 cut(s) | 895, 1181 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1088, 1122 |
| BciT130I | CCWGG | 2 cut(s) | 1324, 2313 |
| BclI | TGATCA | 2 cut(s) | 630, 2233 |
| BcoDI | GTCTC | 4 cut(s) | 540, 989, 1181, 1700 |
| BfaI | CTAG | 4 cut(s) | 12, 192, 1328, 1646 |
| BfmI | CTRYAG | 1 cut(s) | 1884 |
| BfoI | RGCGCY | 1 cut(s) | 2419 |
| BfrI | CTTAAG | 1 cut(s) | 1495 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1165 |
| BlpI | GCTNAGC | 2 cut(s) | 245, 1311 |
| Bme1390I | CCNGG | 2 cut(s) | 1324, 2313 |
| BmeT110I | CYCGRG | 1 cut(s) | 1094 |
| BmgT120I | GGNCC | 1 cut(s) | 2016 |
| BmiI | GGNNCC | 4 cut(s) | 459, 2331, 2403, 2417 |
| BmrFI | CCNGG | 2 cut(s) | 1324, 2313 |
| BmrI | ACTGGG | 1 cut(s) | 1575 |
| BmsI | GCATC | 9 cut(s) | 35, 287, 408, 583, 1108, 1615, 1825, 1882, 2053 |
| BmuI | ACTGGG | 1 cut(s) | 1575 |
| BpiI | GAAGAC | 1 cut(s) | 2601 |
| BpmI | CTGGAG | 1 cut(s) | 2552 |
| Bpu10I | CCTNAGC | 3 cut(s) | 984, 1245, 1290 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 245, 1311 |
| BsaAI | YACGTR | 2 cut(s) | 812, 866 |
| BsaBI | GATNNNNATC | 2 cut(s) | 85, 524 |
| BsaHI | GRCGYC | 2 cut(s) | 549, 2416 |
| BsaI | GGTCTC | 2 cut(s) | 989, 1700 |
| BsaJI | CCNNGG | 3 cut(s) | 418, 1726, 2121 |
| BsaWI | WCCGGW | 4 cut(s) | 353, 730, 1384, 2240 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 1056, 1548, 1902 |
| Bse118I | RCCGGY | 3 cut(s) | 353, 1384, 2240 |
| Bse1I | ACTGG | 4 cut(s) | 927, 1570, 1988, 2431 |
| Bse3DI | GCAATG | 2 cut(s) | 72, 1875 |
| Bse8I | GATNNNNATC | 2 cut(s) | 85, 524 |
| BseBI | CCWGG | 2 cut(s) | 1324, 2313 |
| BseDI | CCNNGG | 3 cut(s) | 418, 1726, 2121 |
| BseGI | GGATG | 6 cut(s) | 387, 598, 1840, 2041, 2080, 2361 |
| BseJI | GATNNNNATC | 2 cut(s) | 85, 524 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 1056, 1548, 1902 |
| BseMI | GCAATG | 2 cut(s) | 72, 1875 |
| BseMII | CTCAG | 6 cut(s) | 38, 236, 975, 1281, 1452, 2355 |
| BseNI | ACTGG | 4 cut(s) | 927, 1570, 1988, 2431 |
| BseRI | GAGGAG | 6 cut(s) | 1007, 1235, 1238, 1280, 1283, 1595 |
| BseSI | GKGCMC | 2 cut(s) | 2248, 2478 |
| BseXI | GCAGC | 8 cut(s) | 74, 151, 628, 1363, 1726, 1898, 2138, 2240 |
| Bsh1236I | CGCG | 2 cut(s) | 1758, 2274 |
| BshFI | GGCC | 3 cut(s) | 323, 1861, 2018 |
| BshNI | GGYRCC | 2 cut(s) | 2329, 2415 |
| BshTI | ACCGGT | 3 cut(s) | 353, 1384, 2240 |
| BsiHKAI | GWGCWC | 2 cut(s) | 2248, 2478 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1094 |
| BsiSI | CCGG | 4 cut(s) | 354, 731, 1385, 2241 |
| BslFI | GGGAC | 2 cut(s) | 1141, 1858 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 1056, 1548, 1902 |
| BsmAI | GTCTC | 4 cut(s) | 540, 989, 1181, 1700 |
| BsmBI | CGTCTC | 1 cut(s) | 540 |
| BsmFI | GGGAC | 2 cut(s) | 1141, 1858 |
| BsmI | GAATGC | 2 cut(s) | 64, 2526 |
| BsnI | GGCC | 3 cut(s) | 323, 1861, 2018 |
| Bso31I | GGTCTC | 2 cut(s) | 989, 1700 |
| BsoBI | CYCGRG | 1 cut(s) | 1094 |
| Bsp1286I | GDGCHC | 2 cut(s) | 2248, 2478 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 245, 1311 |
| Bsp19I | CCATGG | 2 cut(s) | 1726, 2121 |
| BspACI | CCGC | 5 cut(s) | 397, 999, 2032, 2148, 2256 |
| BspANI | GGCC | 3 cut(s) | 323, 1861, 2018 |
| BspCNI | CTCAG | 6 cut(s) | 39, 237, 976, 1282, 1453, 2354 |
| BspFNI | CGCG | 2 cut(s) | 1758, 2274 |
| BspHI | TCATGA | 2 cut(s) | 331, 1783 |
| BspLI | GGNNCC | 4 cut(s) | 459, 2331, 2403, 2417 |
| BspMAI | CTGCAG | 1 cut(s) | 1888 |
| BspPI | GGATC | 5 cut(s) | 30, 514, 2396, 2409, 2573 |
| BspT107I | GGYRCC | 2 cut(s) | 2329, 2415 |
| BspTI | CTTAAG | 1 cut(s) | 1495 |
| BspTNI | GGTCTC | 2 cut(s) | 989, 1700 |
| BsrDI | GCAATG | 2 cut(s) | 72, 1875 |
| BsrFI | RCCGGY | 3 cut(s) | 353, 1384, 2240 |
| BsrI | ACTGG | 4 cut(s) | 927, 1570, 1988, 2431 |
| BssAI | RCCGGY | 3 cut(s) | 353, 1384, 2240 |
| BssECI | CCNNGG | 3 cut(s) | 418, 1726, 2121 |
| BssNI | GRCGYC | 2 cut(s) | 549, 2416 |
| BssT1I | CCWWGG | 2 cut(s) | 1726, 2121 |
| Bst2UI | CCWGG | 2 cut(s) | 1324, 2313 |
| Bst4CI | ACNGT | 7 cut(s) | 436, 857, 1132, 1192, 1284, 1723, 1828 |
| Bst6I | CTCTTC | 4 cut(s) | 1179, 1572, 2186, 2376 |
| BstACI | GRCGYC | 2 cut(s) | 549, 2416 |
| BstAFI | CTTAAG | 1 cut(s) | 1495 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1892 |
| BstBAI | YACGTR | 2 cut(s) | 812, 866 |
| BstC8I | GCNNGC | 2 cut(s) | 1539, 2524 |
| BstDSI | CCRYGG | 2 cut(s) | 1726, 2121 |
| BstF5I | GGATG | 6 cut(s) | 387, 598, 1840, 2041, 2080, 2361 |
| BstFNI | CGCG | 2 cut(s) | 1758, 2274 |
| BstH2I | RGCGCY | 1 cut(s) | 2419 |
| BstHHI | GCGC | 3 cut(s) | 1758, 2276, 2418 |
| BstMAI | GTCTC | 4 cut(s) | 540, 989, 1181, 1700 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 373, 1165, 1307, 1886, 1892, 2000, 2683 |
| BstNI | CCWGG | 2 cut(s) | 1324, 2313 |
| BstNSI | RCATGY | 5 cut(s) | 1056, 1541, 1595, 2134, 2695 |
| BstSCI | CCNGG | 2 cut(s) | 1322, 2311 |
| BstSFI | CTRYAG | 1 cut(s) | 1884 |
| BstSLI | GKGCMC | 2 cut(s) | 2248, 2478 |
| BstUI | CGCG | 2 cut(s) | 1758, 2274 |
| BstV1I | GCAGC | 8 cut(s) | 74, 151, 628, 1363, 1726, 1898, 2138, 2240 |
| BstV2I | GAAGAC | 1 cut(s) | 2601 |
| BstX2I | RGATCY | 3 cut(s) | 35, 519, 2401 |
| BstYI | RGATCY | 3 cut(s) | 35, 519, 2401 |
| BsuRI | GGCC | 3 cut(s) | 323, 1861, 2018 |
| BtgI | CCRYGG | 2 cut(s) | 1726, 2121 |
| BtsCI | GGATG | 6 cut(s) | 387, 598, 1840, 2041, 2080, 2361 |
| BtsI | GCAGTG | 1 cut(s) | 324 |
| BtsIMutI | CAGTG | 4 cut(s) | 44, 324, 1202, 2022 |
| Cac8I | GCNNGC | 2 cut(s) | 1539, 2524 |
| CaiI | CAGNNNCTG | 2 cut(s) | 29, 1994 |
| CciI | TCATGA | 2 cut(s) | 331, 1783 |
| CfoI | GCGC | 3 cut(s) | 1758, 2276, 2418 |
| Cfr10I | RCCGGY | 3 cut(s) | 353, 1384, 2240 |
| Cfr13I | GGNCC | 1 cut(s) | 2016 |
| CseI | GACGC | 2 cut(s) | 206, 1747 |
| Csp6I | GTAC | 3 cut(s) | 513, 809, 1773 |
| CspAI | ACCGGT | 3 cut(s) | 353, 1384, 2240 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 337, 372, 2536, 2571 |
| CviQI | GTAC | 3 cut(s) | 513, 809, 1773 |
| DinI | GGCGCC | 1 cut(s) | 2417 |
| EaeI | YGGCCR | 1 cut(s) | 1859 |
| Eam1104I | CTCTTC | 4 cut(s) | 1179, 1572, 2186, 2376 |
| EarI | CTCTTC | 4 cut(s) | 1179, 1572, 2186, 2376 |
| Eco130I | CCWWGG | 2 cut(s) | 1726, 2121 |
| Eco31I | GGTCTC | 2 cut(s) | 989, 1700 |
| Eco57I | CTGAAG | 2 cut(s) | 392, 1226 |
| Eco88I | CYCGRG | 1 cut(s) | 1094 |
| EcoRII | CCWGG | 2 cut(s) | 1322, 2311 |
| EcoT14I | CCWWGG | 2 cut(s) | 1726, 2121 |
| EcoT22I | ATGCAT | 1 cut(s) | 598 |
| EgeI | GGCGCC | 1 cut(s) | 2417 |
| EheI | GGCGCC | 1 cut(s) | 2417 |
| ErhI | CCWWGG | 2 cut(s) | 1726, 2121 |
| Esp3I | CGTCTC | 1 cut(s) | 540 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1478, 1510, 1515, 1547 |
| FaqI | GGGAC | 2 cut(s) | 1141, 1858 |
| FauI | CCCGC | 1 cut(s) | 2025 |
| FbaI | TGATCA | 2 cut(s) | 630, 2233 |
| FblI | GTMKAC | 2 cut(s) | 1698, 2226 |
| FokI | GGATG | 6 cut(s) | 394, 605, 1847, 2048, 2067, 2368 |
| FspBI | CTAG | 4 cut(s) | 12, 192, 1328, 1646 |
| GlaI | GCGC | 3 cut(s) | 1757, 2275, 2417 |
| GsuI | CTGGAG | 1 cut(s) | 2552 |
| HaeII | RGCGCY | 1 cut(s) | 2419 |
| HaeIII | GGCC | 3 cut(s) | 323, 1861, 2018 |
| HapII | CCGG | 4 cut(s) | 354, 731, 1385, 2241 |
| HgaI | GACGC | 2 cut(s) | 206, 1747 |
| HhaI | GCGC | 3 cut(s) | 1758, 2276, 2418 |
| Hin1I | GRCGYC | 2 cut(s) | 549, 2416 |
| Hin6I | GCGC | 3 cut(s) | 1756, 2274, 2416 |
| HinP1I | GCGC | 3 cut(s) | 1756, 2274, 2416 |
| HincII | GTYRAC | 3 cut(s) | 675, 1699, 2227 |
| HindII | GTYRAC | 3 cut(s) | 675, 1699, 2227 |
| HindIII | AAGCTT | 2 cut(s) | 198, 1788 |
| HinfI | GANTC | 5 cut(s) | 581, 1090, 1655, 2040, 2371 |
| HpaII | CCGG | 4 cut(s) | 354, 731, 1385, 2241 |
| HphI | GGTGA | 6 cut(s) | 640, 845, 1039, 1442, 2035, 2441 |
| Hpy166II | GTNNAC | 7 cut(s) | 675, 1510, 1699, 2227, 2246, 2476, 2647 |
| Hpy188I | TCNGA | 7 cut(s) | 118, 295, 419, 538, 901, 1063, 1608 |
| Hpy8I | GTNNAC | 7 cut(s) | 675, 1510, 1699, 2227, 2246, 2476, 2647 |
| Hpy99I | CGWCG | 1 cut(s) | 554 |
| HpyAV | CCTTC | 6 cut(s) | 1031, 1222, 1342, 1546, 1603, 2575 |
| HpyCH4III | ACNGT | 7 cut(s) | 436, 857, 1132, 1192, 1284, 1723, 1828 |
| HpyCH4IV | ACGT | 5 cut(s) | 549, 770, 811, 865, 1512 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 373, 1165, 1307, 1886, 1892, 2000, 2683 |
| HpySE526I | ACGT | 5 cut(s) | 549, 770, 811, 865, 1512 |
| Hsp92I | GRCGYC | 2 cut(s) | 549, 2416 |
| HspAI | GCGC | 3 cut(s) | 1756, 2274, 2416 |
| KasI | GGCGCC | 1 cut(s) | 2415 |
| Ksp22I | TGATCA | 2 cut(s) | 630, 2233 |
| LmnI | GCTCC | 2 cut(s) | 1413, 2533 |
| Lsp1109I | GCAGC | 8 cut(s) | 74, 151, 628, 1363, 1726, 1898, 2138, 2240 |
| LweI | GCATC | 9 cut(s) | 35, 287, 408, 583, 1108, 1615, 1825, 1882, 2053 |
| MaeI | CTAG | 4 cut(s) | 12, 192, 1328, 1646 |
| MaeII | ACGT | 5 cut(s) | 549, 770, 811, 865, 1512 |
| MaeIII | GTNAC | 9 cut(s) | 145, 385, 1200, 1759, 1822, 1996, 2041, 2108, 2573 |
| MfeI | CAATTG | 1 cut(s) | 760 |
| MflI | RGATCY | 3 cut(s) | 35, 519, 2401 |
| MhlI | GDGCHC | 2 cut(s) | 2248, 2478 |
| Mly113I | GGCGCC | 1 cut(s) | 2416 |
| MlyI | GAGTC | 2 cut(s) | 590, 2049 |
| MmeI | TCCRAC | 3 cut(s) | 599, 2115, 2288 |
| Mph1103I | ATGCAT | 1 cut(s) | 598 |
| MroXI | GAANNNNTTC | 1 cut(s) | 199 |
| MslI | CAYNNNNRTG | 3 cut(s) | 336, 500, 2241 |
| MspA1I | CMGCKG | 1 cut(s) | 794 |
| MspCI | CTTAAG | 1 cut(s) | 1495 |
| MspI | CCGG | 4 cut(s) | 354, 731, 1385, 2241 |
| MspR9I | CCNGG | 2 cut(s) | 1324, 2313 |
| MunI | CAATTG | 1 cut(s) | 760 |
| Mva1269I | GAATGC | 2 cut(s) | 64, 2526 |
| MvaI | CCWGG | 2 cut(s) | 1324, 2313 |
| MvnI | CGCG | 2 cut(s) | 1758, 2274 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 373, 1165, 1307, 1886, 1892, 2000, 2683 |
| NarI | GGCGCC | 1 cut(s) | 2416 |
| NcoI | CCATGG | 2 cut(s) | 1726, 2121 |
| NlaIV | GGNNCC | 4 cut(s) | 459, 2331, 2403, 2417 |
| NmuCI | GTSAC | 6 cut(s) | 145, 1200, 1759, 1822, 2041, 2108 |
| NsiI | ATGCAT | 1 cut(s) | 598 |
| NspI | RCATGY | 5 cut(s) | 1056, 1541, 1595, 2134, 2695 |
| OliI | CACNNNNGTG | 1 cut(s) | 2241 |
| PaeI | GCATGC | 1 cut(s) | 1541 |
| PagI | TCATGA | 2 cut(s) | 331, 1783 |
| PciI | ACATGT | 4 cut(s) | 1052, 1591, 2130, 2691 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 558 |
| PctI | GAATGC | 2 cut(s) | 64, 2526 |
| PdmI | GAANNNNTTC | 1 cut(s) | 199 |
| PfeI | GAWTC | 3 cut(s) | 1090, 1655, 2371 |
| PinAI | ACCGGT | 3 cut(s) | 353, 1384, 2240 |
| PleI | GAGTC | 2 cut(s) | 589, 2048 |
| PluTI | GGCGCC | 1 cut(s) | 2419 |
| PpsI | GAGTC | 2 cut(s) | 589, 2048 |
| Ppu21I | YACGTR | 2 cut(s) | 812, 866 |
| PscI | ACATGT | 4 cut(s) | 1052, 1591, 2130, 2691 |
| PshBI | ATTAAT | 1 cut(s) | 2501 |
| PsiI | TTATAA | 2 cut(s) | 2181, 2698 |
| Psp1406I | AACGTT | 1 cut(s) | 770 |
| Psp6I | CCWGG | 2 cut(s) | 1322, 2311 |
| PspGI | CCWGG | 2 cut(s) | 1322, 2311 |
| PspN4I | GGNNCC | 4 cut(s) | 459, 2331, 2403, 2417 |
| PspPI | GGNCC | 1 cut(s) | 2016 |
| PstI | CTGCAG | 1 cut(s) | 1888 |
| PstNI | CAGNNNCTG | 2 cut(s) | 29, 1994 |
| PsuI | RGATCY | 3 cut(s) | 35, 519, 2401 |
| PvuII | CAGCTG | 1 cut(s) | 794 |
| RsaI | GTAC | 3 cut(s) | 514, 810, 1774 |
| RsaNI | GTAC | 3 cut(s) | 513, 809, 1773 |
| RseI | CAYNNNNRTG | 3 cut(s) | 336, 500, 2241 |
| SalI | GTCGAC | 2 cut(s) | 1697, 2225 |
| Sau96I | GGNCC | 1 cut(s) | 2016 |
| SchI | GAGTC | 2 cut(s) | 590, 2049 |
| ScrFI | CCNGG | 2 cut(s) | 1324, 2313 |
| SduI | GDGCHC | 2 cut(s) | 2248, 2478 |
| SfaNI | GCATC | 9 cut(s) | 35, 287, 408, 583, 1108, 1615, 1825, 1882, 2053 |
| SfcI | CTRYAG | 1 cut(s) | 1884 |
| SfoI | GGCGCC | 1 cut(s) | 2417 |
| SgrAI | CRCCGGYG | 1 cut(s) | 2240 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 336, 500, 2241 |
| SmlI | CTYRAG | 1 cut(s) | 1495 |
| SmoI | CTYRAG | 1 cut(s) | 1495 |
| SphI | GCATGC | 1 cut(s) | 1541 |
| SsiI | CCGC | 5 cut(s) | 397, 999, 2032, 2148, 2256 |
| SspDI | GGCGCC | 1 cut(s) | 2415 |
| SspI | AATATT | 2 cut(s) | 1215, 2661 |
| SspMI | CTAG | 4 cut(s) | 12, 192, 1328, 1646 |
| StyD4I | CCNGG | 2 cut(s) | 1322, 2311 |
| StyI | CCWWGG | 2 cut(s) | 1726, 2121 |
| TaaI | ACNGT | 7 cut(s) | 436, 857, 1132, 1192, 1284, 1723, 1828 |
| TaiI | ACGT | 5 cut(s) | 552, 773, 814, 868, 1515 |
| TaqI | TCGA | 3 cut(s) | 552, 1698, 2226 |
| TaqII | GACCGA | 1 cut(s) | 2268 |
| TauI | GCSGC | 2 cut(s) | 399, 2151 |
| TfiI | GAWTC | 3 cut(s) | 1090, 1655, 2371 |
| TscAI | CASTG | 4 cut(s) | 51, 331, 1209, 2029 |
| TseFI | GTSAC | 6 cut(s) | 145, 1200, 1759, 1822, 2041, 2108 |
| TseI | GCWGC | 8 cut(s) | 62, 164, 641, 1376, 1714, 1886, 2151, 2253 |
| Tsp45I | GTSAC | 6 cut(s) | 145, 1200, 1759, 1822, 2041, 2108 |
| TspGWI | ACGGA | 2 cut(s) | 605, 2394 |
| TspRI | CASTG | 4 cut(s) | 51, 331, 1209, 2029 |
| Vha464I | CTTAAG | 1 cut(s) | 1495 |
| VneI | GTGCAC | 2 cut(s) | 2244, 2474 |
| VspI | ATTAAT | 1 cut(s) | 2501 |
| XapI | RAATTY | 4 cut(s) | 722, 880, 2070, 2602 |
| XceI | RCATGY | 5 cut(s) | 1056, 1541, 1595, 2134, 2695 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1657 |
| XmiI | GTMKAC | 2 cut(s) | 1698, 2226 |
| XmnI | GAANNNNTTC | 1 cut(s) | 199 |
| XspI | CTAG | 4 cut(s) | 12, 192, 1328, 1646 |
| ZraI | GACGTC | 1 cut(s) | 550 |
| Zsp2I | ATGCAT | 1 cut(s) | 598 |