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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1136 |
| AatII | GACGTC | 1 cut(s) | 1589 |
| Acc36I | ACCTGC | 2 cut(s) | 692, 1136 |
| Acc65I | GGTACC | 2 cut(s) | 331, 1791 |
| AccB1I | GGYRCC | 3 cut(s) | 331, 1594, 1791 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 537 |
| AccI | GTMKAC | 1 cut(s) | 80 |
| AciI | CCGC | 2 cut(s) | 5, 194 |
| AclWI | GGATC | 6 cut(s) | 1211, 1224, 1355, 1441, 1732, 1841 |
| AcoI | YGGCCR | 1 cut(s) | 994 |
| AcsI | RAATTY | 3 cut(s) | 917, 1047, 1646 |
| AcuI | CTGAAG | 3 cut(s) | 519, 1083, 1707 |
| AcyI | GRCGYC | 1 cut(s) | 1586 |
| AfaI | GTAC | 7 cut(s) | 333, 1119, 1200, 1372, 1663, 1784, 1793 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 167, 328, 537, 1231 |
| AflII | CTTAAG | 1 cut(s) | 182 |
| AgsI | TTSAA | 8 cut(s) | 61, 126, 216, 574, 619, 1475, 1618, 1651 |
| AjnI | CCWGG | 2 cut(s) | 1889, 1930 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 731, 763 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1716, 1748 |
| AluBI | AGCT | 8 cut(s) | 615, 902, 1005, 1061, 1196, 1286, 1581, 1713 |
| AluI | AGCT | 8 cut(s) | 615, 902, 1005, 1061, 1196, 1286, 1581, 1713 |
| Alw21I | GWGCWC | 4 cut(s) | 92, 400, 650, 1387 |
| Alw26I | GTCTC | 1 cut(s) | 587 |
| AlwI | GGATC | 6 cut(s) | 1211, 1224, 1355, 1441, 1732, 1841 |
| AoxI | GGCC | 3 cut(s) | 318, 528, 994 |
| ApeKI | GCWGC | 2 cut(s) | 1154, 1424 |
| ApoI | RAATTY | 3 cut(s) | 917, 1047, 1646 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 32, 64 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1006 |
| Asp718I | GGTACC | 2 cut(s) | 331, 1791 |
| AspLEI | GCGC | 1 cut(s) | 189 |
| AspS9I | GGNCC | 3 cut(s) | 319, 528, 1894 |
| AsuC2I | CCSGG | 1 cut(s) | 166 |
| AsuHPI | GGTGA | 4 cut(s) | 208, 422, 1367, 1649 |
| AvaII | GGWCC | 1 cut(s) | 1894 |
| BalI | TGGCCA | 1 cut(s) | 996 |
| BamHI | GGATCC | 1 cut(s) | 1216 |
| BanI | GGYRCC | 3 cut(s) | 331, 1594, 1791 |
| BbsI | GAAGAC | 2 cut(s) | 77, 1566 |
| Bbv12I | GWGCWC | 4 cut(s) | 92, 400, 650, 1387 |
| BbvI | GCAGC | 2 cut(s) | 1166, 1411 |
| BccI | CCATC | 3 cut(s) | 557, 629, 1673 |
| BceAI | ACGGC | 1 cut(s) | 1936 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 686, 720, 1010, 1044 |
| BciT130I | CCWGG | 2 cut(s) | 1891, 1932 |
| BcnI | CCSGG | 1 cut(s) | 166 |
| BcoDI | GTCTC | 1 cut(s) | 587 |
| BfaI | CTAG | 4 cut(s) | 779, 815, 1368, 1764 |
| BfmI | CTRYAG | 1 cut(s) | 1425 |
| BfoI | RGCGCY | 1 cut(s) | 190 |
| BfrI | CTTAAG | 1 cut(s) | 182 |
| BfuAI | ACCTGC | 2 cut(s) | 692, 1136 |
| BisI | GCNGC | 3 cut(s) | 6, 1155, 1425 |
| BlsI | GCNGC | 3 cut(s) | 7, 1156, 1426 |
| Bme1390I | CCNGG | 3 cut(s) | 166, 1891, 1932 |
| Bme18I | GGWCC | 1 cut(s) | 1894 |
| BmgT120I | GGNCC | 3 cut(s) | 319, 528, 1894 |
| BmiI | GGNNCC | 7 cut(s) | 333, 1218, 1315, 1596, 1683, 1793, 1895 |
| BmrFI | CCNGG | 3 cut(s) | 166, 1891, 1932 |
| BmrI | ACTGGG | 1 cut(s) | 1400 |
| BmsI | GCATC | 4 cut(s) | 17, 285, 1141, 1611 |
| BmuI | ACTGGG | 1 cut(s) | 1400 |
| BpiI | GAAGAC | 2 cut(s) | 77, 1566 |
| Bpu10I | CCTNAGC | 4 cut(s) | 288, 399, 676, 693 |
| BpuEI | CTTGAG | 1 cut(s) | 950 |
| BpuMI | CCSGG | 1 cut(s) | 166 |
| Bsa29I | ATCGAT | 1 cut(s) | 522 |
| BsaAI | YACGTR | 1 cut(s) | 395 |
| BsaHI | GRCGYC | 1 cut(s) | 1586 |
| BsaJI | CCNNGG | 6 cut(s) | 165, 558, 997, 1053, 1890, 1931 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 284, 314, 476, 506 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 167, 328, 537, 1231 |
| Bse1I | ACTGG | 2 cut(s) | 118, 1395 |
| Bse3DI | GCAATG | 3 cut(s) | 1157, 1256, 1402 |
| BseBI | CCWGG | 2 cut(s) | 1891, 1932 |
| BseCI | ATCGAT | 1 cut(s) | 522 |
| BseDI | CCNNGG | 6 cut(s) | 165, 558, 997, 1053, 1890, 1931 |
| BseGI | GGATG | 5 cut(s) | 514, 568, 583, 1120, 1180 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 167, 328, 537, 1231 |
| BseMI | GCAATG | 3 cut(s) | 1157, 1256, 1402 |
| BseMII | CTCAG | 4 cut(s) | 413, 635, 684, 1278 |
| BseNI | ACTGG | 2 cut(s) | 118, 1395 |
| BseRI | GAGGAG | 5 cut(s) | 386, 836, 881, 1244, 1448 |
| BseXI | GCAGC | 2 cut(s) | 1166, 1411 |
| BsgI | GTGCAG | 2 cut(s) | 824, 1884 |
| BshFI | GGCC | 3 cut(s) | 320, 530, 996 |
| BshNI | GGYRCC | 3 cut(s) | 331, 1594, 1791 |
| BshVI | ATCGAT | 1 cut(s) | 522 |
| BsiHKAI | GWGCWC | 4 cut(s) | 92, 400, 650, 1387 |
| BsiSI | CCGG | 1 cut(s) | 165 |
| BslFI | GGGAC | 2 cut(s) | 445, 700 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 167, 328, 537, 1231 |
| BsmAI | GTCTC | 1 cut(s) | 587 |
| BsmFI | GGGAC | 2 cut(s) | 445, 700 |
| BsmI | GAATGC | 1 cut(s) | 1444 |
| BsnI | GGCC | 3 cut(s) | 320, 530, 996 |
| Bsp1286I | GDGCHC | 4 cut(s) | 92, 400, 650, 1387 |
| Bsp143I | GATC | 5 cut(s) | 1216, 1360, 1446, 1724, 1846 |
| BspACI | CCGC | 2 cut(s) | 5, 194 |
| BspANI | GGCC | 3 cut(s) | 320, 530, 996 |
| BspCNI | CTCAG | 4 cut(s) | 412, 636, 685, 1279 |
| BspDI | ATCGAT | 1 cut(s) | 522 |
| BspLI | GGNNCC | 7 cut(s) | 333, 1218, 1315, 1596, 1683, 1793, 1895 |
| BspMAI | CTGCAG | 1 cut(s) | 1429 |
| BspMI | ACCTGC | 2 cut(s) | 692, 1136 |
| BspPI | GGATC | 6 cut(s) | 1211, 1224, 1355, 1441, 1732, 1841 |
| BspT107I | GGYRCC | 3 cut(s) | 331, 1594, 1791 |
| BspTI | CTTAAG | 1 cut(s) | 182 |
| BsrDI | GCAATG | 3 cut(s) | 1157, 1256, 1402 |
| BsrI | ACTGG | 2 cut(s) | 118, 1395 |
| BssECI | CCNNGG | 6 cut(s) | 165, 558, 997, 1053, 1890, 1931 |
| BssMI | GATC | 5 cut(s) | 1216, 1360, 1446, 1724, 1846 |
| BssNI | GRCGYC | 1 cut(s) | 1586 |
| BssT1I | CCWWGG | 3 cut(s) | 558, 997, 1053 |
| Bst2UI | CCWGG | 2 cut(s) | 1891, 1932 |
| Bst4CI | ACNGT | 6 cut(s) | 247, 790, 973, 1345, 1375, 1480 |
| Bst6I | CTCTTC | 2 cut(s) | 483, 1526 |
| BstACI | GRCGYC | 1 cut(s) | 1586 |
| BstAFI | CTTAAG | 1 cut(s) | 182 |
| BstBAI | YACGTR | 1 cut(s) | 395 |
| BstC8I | GCNNGC | 4 cut(s) | 699, 900, 1488, 1747 |
| BstDEI | CTNAG | 7 cut(s) | 66, 288, 399, 644, 676, 693, 1287 |
| BstF5I | GGATG | 5 cut(s) | 514, 568, 583, 1120, 1180 |
| BstH2I | RGCGCY | 1 cut(s) | 190 |
| BstHHI | GCGC | 1 cut(s) | 189 |
| BstKTI | GATC | 5 cut(s) | 1219, 1363, 1449, 1727, 1849 |
| BstMAI | GTCTC | 1 cut(s) | 587 |
| BstMBI | GATC | 5 cut(s) | 1216, 1360, 1446, 1724, 1846 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 645, 920, 1002, 1011 |
| BstNI | CCWGG | 2 cut(s) | 1891, 1932 |
| BstNSI | RCATGY | 1 cut(s) | 701 |
| BstSCI | CCNGG | 3 cut(s) | 164, 1889, 1930 |
| BstSFI | CTRYAG | 1 cut(s) | 1425 |
| BstV1I | GCAGC | 2 cut(s) | 1166, 1411 |
| BstV2I | GAAGAC | 2 cut(s) | 77, 1566 |
| BstX2I | RGATCY | 2 cut(s) | 1216, 1446 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1348 |
| BstYI | RGATCY | 2 cut(s) | 1216, 1446 |
| Bsu15I | ATCGAT | 1 cut(s) | 522 |
| BsuRI | GGCC | 3 cut(s) | 320, 530, 996 |
| BsuTUI | ATCGAT | 1 cut(s) | 522 |
| BtsCI | GGATG | 5 cut(s) | 514, 568, 583, 1120, 1180 |
| BtsIMutI | CAGTG | 2 cut(s) | 1341, 1388 |
| BveI | ACCTGC | 2 cut(s) | 692, 1136 |
| Cac8I | GCNNGC | 4 cut(s) | 699, 900, 1488, 1747 |
| CfoI | GCGC | 1 cut(s) | 189 |
| Cfr13I | GGNCC | 3 cut(s) | 319, 528, 1894 |
| ClaI | ATCGAT | 1 cut(s) | 522 |
| CseI | GACGC | 1 cut(s) | 1151 |
| Csp6I | GTAC | 7 cut(s) | 332, 1118, 1199, 1371, 1662, 1783, 1792 |
| CviQI | GTAC | 7 cut(s) | 332, 1118, 1199, 1371, 1662, 1783, 1792 |
| DdeI | CTNAG | 7 cut(s) | 66, 288, 399, 644, 676, 693, 1287 |
| DpnI | GATC | 5 cut(s) | 1218, 1362, 1448, 1726, 1848 |
| DpnII | GATC | 5 cut(s) | 1216, 1360, 1446, 1724, 1846 |
| EaeI | YGGCCR | 1 cut(s) | 994 |
| Eam1104I | CTCTTC | 2 cut(s) | 483, 1526 |
| EarI | CTCTTC | 2 cut(s) | 483, 1526 |
| Eco130I | CCWWGG | 3 cut(s) | 558, 997, 1053 |
| Eco47I | GGWCC | 1 cut(s) | 1894 |
| Eco57I | CTGAAG | 3 cut(s) | 519, 1083, 1707 |
| EcoRII | CCWGG | 2 cut(s) | 1889, 1930 |
| EcoT14I | CCWWGG | 3 cut(s) | 558, 997, 1053 |
| ErhI | CCWWGG | 3 cut(s) | 558, 997, 1053 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1899, 1931 |
| FaqI | GGGAC | 2 cut(s) | 445, 700 |
| FblI | GTMKAC | 1 cut(s) | 80 |
| Fnu4HI | GCNGC | 3 cut(s) | 6, 1155, 1425 |
| FokI | GGATG | 5 cut(s) | 521, 575, 590, 1107, 1187 |
| Fsp4HI | GCNGC | 3 cut(s) | 6, 1155, 1425 |
| FspBI | CTAG | 4 cut(s) | 779, 815, 1368, 1764 |
| GlaI | GCGC | 1 cut(s) | 188 |
| GluI | GCNGC | 3 cut(s) | 6, 1155, 1425 |
| HaeII | RGCGCY | 1 cut(s) | 190 |
| HaeIII | GGCC | 3 cut(s) | 320, 530, 996 |
| HapII | CCGG | 1 cut(s) | 165 |
| HgaI | GACGC | 1 cut(s) | 1151 |
| HhaI | GCGC | 1 cut(s) | 189 |
| Hin1I | GRCGYC | 1 cut(s) | 1586 |
| Hin6I | GCGC | 1 cut(s) | 187 |
| HinP1I | GCGC | 1 cut(s) | 187 |
| HindIII | AAGCTT | 2 cut(s) | 613, 900 |
| HinfI | GANTC | 5 cut(s) | 427, 688, 1168, 1562, 1610 |
| HpaII | CCGG | 1 cut(s) | 165 |
| HphI | GGTGA | 4 cut(s) | 208, 422, 1367, 1649 |
| Hpy166II | GTNNAC | 4 cut(s) | 81, 1010, 1783, 1828 |
| Hpy188I | TCNGA | 3 cut(s) | 545, 1167, 1729 |
| Hpy188III | TCNNGA | 5 cut(s) | 401, 670, 692, 1172, 1220 |
| Hpy8I | GTNNAC | 4 cut(s) | 81, 1010, 1783, 1828 |
| Hpy99I | CGWCG | 1 cut(s) | 1740 |
| HpyAV | CCTTC | 7 cut(s) | 28, 274, 429, 1061, 1079, 1374, 1860 |
| HpyCH4III | ACNGT | 6 cut(s) | 247, 790, 973, 1345, 1375, 1480 |
| HpyCH4IV | ACGT | 2 cut(s) | 394, 1586 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 645, 920, 1002, 1011 |
| HpyF3I | CTNAG | 7 cut(s) | 66, 288, 399, 644, 676, 693, 1287 |
| HpySE526I | ACGT | 2 cut(s) | 394, 1586 |
| Hsp92I | GRCGYC | 1 cut(s) | 1586 |
| HspAI | GCGC | 1 cut(s) | 187 |
| KpnI | GGTACC | 2 cut(s) | 335, 1795 |
| Kzo9I | GATC | 5 cut(s) | 1216, 1360, 1446, 1724, 1846 |
| LmnI | GCTCC | 1 cut(s) | 1390 |
| Lsp1109I | GCAGC | 2 cut(s) | 1166, 1411 |
| LweI | GCATC | 4 cut(s) | 17, 285, 1141, 1611 |
| MaeI | CTAG | 4 cut(s) | 779, 815, 1368, 1764 |
| MaeII | ACGT | 2 cut(s) | 394, 1586 |
| MaeIII | GTNAC | 4 cut(s) | 196, 725, 1759, 1918 |
| MalI | GATC | 5 cut(s) | 1218, 1362, 1448, 1726, 1848 |
| MboI | GATC | 5 cut(s) | 1216, 1360, 1446, 1724, 1846 |
| MflI | RGATCY | 2 cut(s) | 1216, 1446 |
| MhlI | GDGCHC | 4 cut(s) | 92, 400, 650, 1387 |
| MlsI | TGGCCA | 1 cut(s) | 996 |
| MluNI | TGGCCA | 1 cut(s) | 996 |
| MlyI | GAGTC | 2 cut(s) | 682, 1556 |
| MmeI | TCCRAC | 2 cut(s) | 1481, 1921 |
| Mox20I | TGGCCA | 1 cut(s) | 996 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1006 |
| MscI | TGGCCA | 1 cut(s) | 996 |
| MseI | TTAA | 4 cut(s) | 183, 255, 1482, 1956 |
| MslI | CAYNNNNRTG | 5 cut(s) | 963, 984, 1346, 1778, 1860 |
| Msp20I | TGGCCA | 1 cut(s) | 996 |
| MspCI | CTTAAG | 1 cut(s) | 182 |
| MspI | CCGG | 1 cut(s) | 165 |
| MspR9I | CCNGG | 3 cut(s) | 166, 1891, 1932 |
| Mva1269I | GAATGC | 1 cut(s) | 1444 |
| MvaI | CCWGG | 2 cut(s) | 1891, 1932 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 645, 920, 1002, 1011 |
| NciI | CCSGG | 1 cut(s) | 166 |
| NdeII | GATC | 5 cut(s) | 1216, 1360, 1446, 1724, 1846 |
| NlaIV | GGNNCC | 7 cut(s) | 333, 1218, 1315, 1596, 1683, 1793, 1895 |
| NmuCI | GTSAC | 2 cut(s) | 196, 1759 |
| NspI | RCATGY | 1 cut(s) | 701 |
| PaeI | GCATGC | 1 cut(s) | 701 |
| PaqCI | CACCTGC | 1 cut(s) | 1136 |
| PctI | GAATGC | 1 cut(s) | 1444 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1006 |
| PfeI | GAWTC | 3 cut(s) | 427, 1168, 1610 |
| PflFI | GACNNNGTC | 2 cut(s) | 76, 202 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 537 |
| PkrI | GCNGC | 3 cut(s) | 7, 1156, 1426 |
| PleI | GAGTC | 2 cut(s) | 682, 1556 |
| PpsI | GAGTC | 2 cut(s) | 682, 1556 |
| Ppu21I | YACGTR | 1 cut(s) | 395 |
| Psp6I | CCWGG | 2 cut(s) | 1889, 1930 |
| PspGI | CCWGG | 2 cut(s) | 1889, 1930 |
| PspN4I | GGNNCC | 7 cut(s) | 333, 1218, 1315, 1596, 1683, 1793, 1895 |
| PspPI | GGNCC | 3 cut(s) | 319, 528, 1894 |
| PstI | CTGCAG | 1 cut(s) | 1429 |
| PsuI | RGATCY | 2 cut(s) | 1216, 1446 |
| PsyI | GACNNNGTC | 2 cut(s) | 76, 202 |
| RsaI | GTAC | 7 cut(s) | 333, 1119, 1200, 1372, 1663, 1784, 1793 |
| RsaNI | GTAC | 7 cut(s) | 332, 1118, 1199, 1371, 1662, 1783, 1792 |
| RseI | CAYNNNNRTG | 5 cut(s) | 963, 984, 1346, 1778, 1860 |
| SaqAI | TTAA | 4 cut(s) | 183, 255, 1482, 1956 |
| SatI | GCNGC | 3 cut(s) | 6, 1155, 1425 |
| Sau3AI | GATC | 5 cut(s) | 1216, 1360, 1446, 1724, 1846 |
| Sau96I | GGNCC | 3 cut(s) | 319, 528, 1894 |
| SchI | GAGTC | 2 cut(s) | 682, 1556 |
| ScrFI | CCNGG | 3 cut(s) | 166, 1891, 1932 |
| SduI | GDGCHC | 4 cut(s) | 92, 400, 650, 1387 |
| SfaNI | GCATC | 4 cut(s) | 17, 285, 1141, 1611 |
| SfcI | CTRYAG | 1 cut(s) | 1425 |
| SinI | GGWCC | 1 cut(s) | 1894 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 963, 984, 1346, 1778, 1860 |
| SmlI | CTYRAG | 2 cut(s) | 182, 929 |
| SmoI | CTYRAG | 2 cut(s) | 182, 929 |
| SphI | GCATGC | 1 cut(s) | 701 |
| SsiI | CCGC | 2 cut(s) | 5, 194 |
| SspMI | CTAG | 4 cut(s) | 779, 815, 1368, 1764 |
| StyD4I | CCNGG | 3 cut(s) | 164, 1889, 1930 |
| StyI | CCWWGG | 3 cut(s) | 558, 997, 1053 |
| TaaI | ACNGT | 6 cut(s) | 247, 790, 973, 1345, 1375, 1480 |
| TaiI | ACGT | 2 cut(s) | 397, 1589 |
| TaqI | TCGA | 5 cut(s) | 44, 522, 706, 1565, 1735 |
| TatI | WGTACW | 1 cut(s) | 1370 |
| TauI | GCSGC | 1 cut(s) | 8 |
| TfiI | GAWTC | 3 cut(s) | 427, 1168, 1610 |
| Tru1I | TTAA | 4 cut(s) | 183, 255, 1482, 1956 |
| Tru9I | TTAA | 4 cut(s) | 183, 255, 1482, 1956 |
| TscAI | CASTG | 2 cut(s) | 1348, 1395 |
| TseFI | GTSAC | 2 cut(s) | 196, 1759 |
| TseI | GCWGC | 2 cut(s) | 1154, 1424 |
| Tsp45I | GTSAC | 2 cut(s) | 196, 1759 |
| TspDTI | ATGAA | 8 cut(s) | 171, 1088, 1278, 1367, 1482, 1602, 1705, 1933 |
| TspGWI | ACGGA | 2 cut(s) | 110, 778 |
| TspRI | CASTG | 2 cut(s) | 1348, 1395 |
| Tth111I | GACNNNGTC | 2 cut(s) | 76, 202 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 537 |
| Vha464I | CTTAAG | 1 cut(s) | 182 |
| VpaK11BI | GGWCC | 1 cut(s) | 1894 |
| XapI | RAATTY | 3 cut(s) | 917, 1047, 1646 |
| XceI | RCATGY | 1 cut(s) | 701 |
| XmiI | GTMKAC | 1 cut(s) | 80 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1006 |
| XspI | CTAG | 4 cut(s) | 779, 815, 1368, 1764 |
| ZraI | GACGTC | 1 cut(s) | 1587 |