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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AgsI | TTSAA | 2 cut(s) | 26, 143 |
| AluBI | AGCT | 1 cut(s) | 150 |
| AluI | AGCT | 1 cut(s) | 150 |
| AoxI | GGCC | 1 cut(s) | 111 |
| AspS9I | GGNCC | 2 cut(s) | 106, 111 |
| AsuC2I | CCSGG | 1 cut(s) | 110 |
| AvaII | GGWCC | 1 cut(s) | 106 |
| BccI | CCATC | 1 cut(s) | 27 |
| BclI | TGATCA | 1 cut(s) | 100 |
| BcnI | CCSGG | 1 cut(s) | 110 |
| BfaI | CTAG | 1 cut(s) | 151 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 119 |
| Bme1390I | CCNGG | 1 cut(s) | 110 |
| Bme18I | GGWCC | 1 cut(s) | 106 |
| BmgT120I | GGNCC | 2 cut(s) | 106, 111 |
| BmrFI | CCNGG | 1 cut(s) | 110 |
| BpuMI | CCSGG | 1 cut(s) | 110 |
| BseGI | GGATG | 1 cut(s) | 38 |
| BshFI | GGCC | 1 cut(s) | 113 |
| BsiSI | CCGG | 1 cut(s) | 109 |
| BsnI | GGCC | 1 cut(s) | 113 |
| Bsp143I | GATC | 1 cut(s) | 100 |
| BspANI | GGCC | 1 cut(s) | 113 |
| BssMI | GATC | 1 cut(s) | 100 |
| BstF5I | GGATG | 1 cut(s) | 38 |
| BstKTI | GATC | 1 cut(s) | 103 |
| BstMBI | GATC | 1 cut(s) | 100 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 119 |
| BstSCI | CCNGG | 1 cut(s) | 108 |
| BsuRI | GGCC | 1 cut(s) | 113 |
| BtsCI | GGATG | 1 cut(s) | 38 |
| BtsIMutI | CAGTG | 1 cut(s) | 62 |
| Cfr13I | GGNCC | 2 cut(s) | 106, 111 |
| CviJI | RGCY | 3 cut(s) | 113, 135, 150 |
| CviKI_1 | RGCY | 3 cut(s) | 113, 135, 150 |
| DpnI | GATC | 1 cut(s) | 102 |
| DpnII | GATC | 1 cut(s) | 100 |
| Eco47I | GGWCC | 1 cut(s) | 106 |
| FaiI | YATR | 1 cut(s) | 129 |
| FbaI | TGATCA | 1 cut(s) | 100 |
| FokI | GGATG | 1 cut(s) | 45 |
| FspBI | CTAG | 1 cut(s) | 151 |
| HaeIII | GGCC | 1 cut(s) | 113 |
| HapII | CCGG | 1 cut(s) | 109 |
| HpaII | CCGG | 1 cut(s) | 109 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 119 |
| Ksp22I | TGATCA | 1 cut(s) | 100 |
| Kzo9I | GATC | 1 cut(s) | 100 |
| LpnPI | CCDG | 2 cut(s) | 89, 122 |
| MaeI | CTAG | 1 cut(s) | 151 |
| MalI | GATC | 1 cut(s) | 102 |
| MboI | GATC | 1 cut(s) | 100 |
| MboII | GAAGA | 1 cut(s) | 16 |
| MluCI | AATT | 1 cut(s) | 137 |
| MspI | CCGG | 1 cut(s) | 109 |
| MspR9I | CCNGG | 1 cut(s) | 110 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 119 |
| NciI | CCSGG | 1 cut(s) | 110 |
| NdeII | GATC | 1 cut(s) | 100 |
| NmeAIII | GCCGAG | 1 cut(s) | 139 |
| PspPI | GGNCC | 2 cut(s) | 106, 111 |
| Sau3AI | GATC | 1 cut(s) | 100 |
| Sau96I | GGNCC | 2 cut(s) | 106, 111 |
| ScrFI | CCNGG | 1 cut(s) | 110 |
| SetI | ASST | 3 cut(s) | 24, 108, 152 |
| SgeI | CNNG | 6 cut(s) | 35, 58, 116, 121, 122, 127 |
| SinI | GGWCC | 1 cut(s) | 106 |
| Sse9I | AATT | 1 cut(s) | 137 |
| SspMI | CTAG | 1 cut(s) | 151 |
| StyD4I | CCNGG | 1 cut(s) | 108 |
| TasI | AATT | 1 cut(s) | 137 |
| TscAI | CASTG | 1 cut(s) | 62 |
| TspDTI | ATGAA | 1 cut(s) | 17 |
| TspRI | CASTG | 1 cut(s) | 62 |
| VpaK11BI | GGWCC | 1 cut(s) | 106 |
| XspI | CTAG | 1 cut(s) | 151 |