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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 1121 |
| AccB1I | GGYRCC | 1 cut(s) | 1121 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1670 |
| AccI | GTMKAC | 1 cut(s) | 1092 |
| AccII | CGCG | 2 cut(s) | 529, 1117 |
| AciI | CCGC | 1 cut(s) | 851 |
| AclWI | GGATC | 5 cut(s) | 88, 482, 495, 1637, 1683 |
| AcuI | CTGAAG | 1 cut(s) | 1239 |
| AcyI | GRCGYC | 1 cut(s) | 742 |
| AfaI | GTAC | 5 cut(s) | 192, 931, 1123, 1128, 1589 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 187, 1381, 1670 |
| AflII | CTTAAG | 1 cut(s) | 271 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 366, 398 |
| AluBI | AGCT | 3 cut(s) | 1088, 1274, 1537 |
| AluI | AGCT | 3 cut(s) | 1088, 1274, 1537 |
| Alw21I | GWGCWC | 1 cut(s) | 1723 |
| Alw26I | GTCTC | 1 cut(s) | 400 |
| AlwI | GGATC | 5 cut(s) | 88, 482, 495, 1637, 1683 |
| AoxI | GGCC | 5 cut(s) | 165, 564, 904, 1267, 1295 |
| ApeKI | GCWGC | 5 cut(s) | 26, 71, 334, 863, 875 |
| Asp718I | GGTACC | 1 cut(s) | 1121 |
| AspLEI | GCGC | 2 cut(s) | 529, 1117 |
| AspS9I | GGNCC | 1 cut(s) | 905 |
| AsuHPI | GGTGA | 2 cut(s) | 107, 1488 |
| AxyI | CCTNAGG | 1 cut(s) | 492 |
| BamHI | GGATCC | 1 cut(s) | 487 |
| BanI | GGYRCC | 1 cut(s) | 1121 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1515, 1547 |
| BauI | CACGAG | 1 cut(s) | 36 |
| Bbv12I | GWGCWC | 1 cut(s) | 1723 |
| BbvI | GCAGC | 5 cut(s) | 38, 83, 321, 875, 887 |
| BccI | CCATC | 5 cut(s) | 71, 608, 626, 917, 1282 |
| BceAI | ACGGC | 1 cut(s) | 1407 |
| BciVI | GTATCC | 1 cut(s) | 435 |
| BclI | TGATCA | 1 cut(s) | 1729 |
| BcoDI | GTCTC | 1 cut(s) | 400 |
| BfaI | CTAG | 5 cut(s) | 738, 966, 1130, 1307, 1335 |
| BfmI | CTRYAG | 3 cut(s) | 335, 1263, 1275 |
| BfrI | CTTAAG | 1 cut(s) | 271 |
| BfuI | GTATCC | 1 cut(s) | 435 |
| BglII | AGATCT | 1 cut(s) | 832 |
| BisI | GCNGC | 5 cut(s) | 27, 72, 335, 864, 876 |
| BlsI | GCNGC | 5 cut(s) | 28, 73, 336, 865, 877 |
| BmgT120I | GGNCC | 1 cut(s) | 905 |
| BmiI | GGNNCC | 4 cut(s) | 371, 489, 907, 1123 |
| BmsI | GCATC | 3 cut(s) | 217, 962, 1129 |
| Bsa29I | ATCGAT | 1 cut(s) | 501 |
| BsaBI | GATNNNNATC | 1 cut(s) | 501 |
| BsaHI | GRCGYC | 1 cut(s) | 742 |
| BsaJI | CCNNGG | 4 cut(s) | 147, 654, 981, 1664 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 693, 723 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 187, 1381, 1670 |
| Bse118I | RCCGGY | 1 cut(s) | 163 |
| Bse21I | CCTNAGG | 1 cut(s) | 492 |
| Bse3DI | GCAATG | 1 cut(s) | 1189 |
| Bse8I | GATNNNNATC | 1 cut(s) | 501 |
| BseCI | ATCGAT | 1 cut(s) | 501 |
| BseDI | CCNNGG | 4 cut(s) | 147, 654, 981, 1664 |
| BseGI | GGATG | 5 cut(s) | 116, 232, 624, 668, 909 |
| BseJI | GATNNNNATC | 1 cut(s) | 501 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 187, 1381, 1670 |
| BseMI | GCAATG | 1 cut(s) | 1189 |
| BseMII | CTCAG | 2 cut(s) | 948, 950 |
| BseRI | GAGGAG | 3 cut(s) | 708, 815, 924 |
| BseXI | GCAGC | 5 cut(s) | 38, 83, 321, 875, 887 |
| BseYI | CCCAGC | 2 cut(s) | 208, 944 |
| BsgI | GTGCAG | 1 cut(s) | 45 |
| Bsh1236I | CGCG | 2 cut(s) | 529, 1117 |
| Bsh1285I | CGRYCG | 1 cut(s) | 505 |
| BshFI | GGCC | 5 cut(s) | 167, 566, 906, 1269, 1297 |
| BshNI | GGYRCC | 1 cut(s) | 1121 |
| BshVI | ATCGAT | 1 cut(s) | 501 |
| BsiEI | CGRYCG | 1 cut(s) | 505 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1723 |
| BsiSI | CCGG | 2 cut(s) | 164, 1758 |
| BslFI | GGGAC | 1 cut(s) | 1687 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 187, 1381, 1670 |
| BsmAI | GTCTC | 1 cut(s) | 400 |
| BsmBI | CGTCTC | 1 cut(s) | 400 |
| BsmFI | GGGAC | 1 cut(s) | 1687 |
| BsmI | GAATGC | 2 cut(s) | 955, 1401 |
| BsnI | GGCC | 5 cut(s) | 167, 566, 906, 1269, 1297 |
| Bsp1286I | GDGCHC | 1 cut(s) | 1723 |
| Bsp143I | GATC | 7 cut(s) | 80, 487, 502, 832, 1642, 1688, 1729 |
| Bsp19I | CCATGG | 3 cut(s) | 147, 654, 1664 |
| BspACI | CCGC | 1 cut(s) | 851 |
| BspANI | GGCC | 5 cut(s) | 167, 566, 906, 1269, 1297 |
| BspCNI | CTCAG | 2 cut(s) | 949, 949 |
| BspDI | ATCGAT | 1 cut(s) | 501 |
| BspFNI | CGCG | 2 cut(s) | 529, 1117 |
| BspHI | TCATGA | 1 cut(s) | 1027 |
| BspLI | GGNNCC | 4 cut(s) | 371, 489, 907, 1123 |
| BspMAI | CTGCAG | 1 cut(s) | 339 |
| BspPI | GGATC | 5 cut(s) | 88, 482, 495, 1637, 1683 |
| BspT107I | GGYRCC | 1 cut(s) | 1121 |
| BspTI | CTTAAG | 1 cut(s) | 271 |
| BsrDI | GCAATG | 1 cut(s) | 1189 |
| BsrFI | RCCGGY | 1 cut(s) | 163 |
| BssAI | RCCGGY | 1 cut(s) | 163 |
| BssECI | CCNNGG | 4 cut(s) | 147, 654, 981, 1664 |
| BssMI | GATC | 7 cut(s) | 80, 487, 502, 832, 1642, 1688, 1729 |
| BssNI | GRCGYC | 1 cut(s) | 742 |
| BssSI | CACGAG | 1 cut(s) | 36 |
| BssT1I | CCWWGG | 4 cut(s) | 147, 654, 981, 1664 |
| Bst2BI | CACGAG | 1 cut(s) | 36 |
| Bst4CI | ACNGT | 4 cut(s) | 444, 721, 1126, 1264 |
| Bst6I | CTCTTC | 5 cut(s) | 679, 742, 756, 1473, 1602 |
| BstACI | GRCGYC | 1 cut(s) | 742 |
| BstAFI | CTTAAG | 1 cut(s) | 271 |
| BstC8I | GCNNGC | 1 cut(s) | 169 |
| BstDEI | CTNAG | 3 cut(s) | 492, 936, 957 |
| BstDSI | CCRYGG | 3 cut(s) | 147, 654, 1664 |
| BstEII | GGTNACC | 1 cut(s) | 1494 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 185 |
| BstF5I | GGATG | 5 cut(s) | 116, 232, 624, 668, 909 |
| BstFNI | CGCG | 2 cut(s) | 529, 1117 |
| BstHHI | GCGC | 2 cut(s) | 529, 1117 |
| BstKTI | GATC | 7 cut(s) | 83, 490, 505, 835, 1645, 1691, 1732 |
| BstMAI | GTCTC | 1 cut(s) | 400 |
| BstMBI | GATC | 7 cut(s) | 80, 487, 502, 832, 1642, 1688, 1729 |
| BstMCI | CGRYCG | 1 cut(s) | 505 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 214, 343, 872, 950 |
| BstPI | GGTNACC | 1 cut(s) | 1494 |
| BstSFI | CTRYAG | 3 cut(s) | 335, 1263, 1275 |
| BstUI | CGCG | 2 cut(s) | 529, 1117 |
| BstV1I | GCAGC | 5 cut(s) | 38, 83, 321, 875, 887 |
| BstX2I | RGATCY | 3 cut(s) | 487, 832, 1688 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 574, 661 |
| BstYI | RGATCY | 3 cut(s) | 487, 832, 1688 |
| Bsu15I | ATCGAT | 1 cut(s) | 501 |
| Bsu36I | CCTNAGG | 1 cut(s) | 492 |
| BsuI | GTATCC | 1 cut(s) | 435 |
| BsuRI | GGCC | 5 cut(s) | 167, 566, 906, 1269, 1297 |
| BsuTUI | ATCGAT | 1 cut(s) | 501 |
| BtgI | CCRYGG | 3 cut(s) | 147, 654, 1664 |
| BtsCI | GGATG | 5 cut(s) | 116, 232, 624, 668, 909 |
| BtsIMutI | CAGTG | 1 cut(s) | 1260 |
| Cac8I | GCNNGC | 1 cut(s) | 169 |
| CciI | TCATGA | 1 cut(s) | 1027 |
| CfoI | GCGC | 2 cut(s) | 529, 1117 |
| Cfr10I | RCCGGY | 1 cut(s) | 163 |
| Cfr13I | GGNCC | 1 cut(s) | 905 |
| ClaI | ATCGAT | 1 cut(s) | 501 |
| CseI | GACGC | 2 cut(s) | 750, 1223 |
| Csp6I | GTAC | 5 cut(s) | 191, 930, 1122, 1127, 1588 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 311, 346 |
| CviQI | GTAC | 5 cut(s) | 191, 930, 1122, 1127, 1588 |
| DdeI | CTNAG | 3 cut(s) | 492, 936, 957 |
| DpnI | GATC | 7 cut(s) | 82, 489, 504, 834, 1644, 1690, 1731 |
| DpnII | GATC | 7 cut(s) | 80, 487, 502, 832, 1642, 1688, 1729 |
| DraI | TTTAAA | 1 cut(s) | 316 |
| Eam1104I | CTCTTC | 5 cut(s) | 679, 742, 756, 1473, 1602 |
| EarI | CTCTTC | 5 cut(s) | 679, 742, 756, 1473, 1602 |
| EciI | GGCGGA | 1 cut(s) | 866 |
| Eco130I | CCWWGG | 4 cut(s) | 147, 654, 981, 1664 |
| Eco32I | GATATC | 2 cut(s) | 499, 1528 |
| Eco57I | CTGAAG | 1 cut(s) | 1239 |
| Eco81I | CCTNAGG | 1 cut(s) | 492 |
| Eco91I | GGTNACC | 1 cut(s) | 1494 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 185 |
| EcoO65I | GGTNACC | 1 cut(s) | 1494 |
| EcoRV | GATATC | 2 cut(s) | 499, 1528 |
| EcoT14I | CCWWGG | 4 cut(s) | 147, 654, 981, 1664 |
| EcoT22I | ATGCAT | 1 cut(s) | 955 |
| ErhI | CCWWGG | 4 cut(s) | 147, 654, 981, 1664 |
| Esp3I | CGTCTC | 1 cut(s) | 400 |
| FaqI | GGGAC | 1 cut(s) | 1687 |
| FauNDI | CATATG | 2 cut(s) | 282, 348 |
| FbaI | TGATCA | 1 cut(s) | 1729 |
| FblI | GTMKAC | 1 cut(s) | 1092 |
| Fnu4HI | GCNGC | 5 cut(s) | 27, 72, 335, 864, 876 |
| FokI | GGATG | 5 cut(s) | 123, 239, 631, 655, 896 |
| Fsp4HI | GCNGC | 5 cut(s) | 27, 72, 335, 864, 876 |
| FspBI | CTAG | 5 cut(s) | 738, 966, 1130, 1307, 1335 |
| GlaI | GCGC | 2 cut(s) | 528, 1116 |
| GluI | GCNGC | 5 cut(s) | 27, 72, 335, 864, 876 |
| GsaI | CCCAGC | 2 cut(s) | 212, 948 |
| HaeIII | GGCC | 5 cut(s) | 167, 566, 906, 1269, 1297 |
| HapII | CCGG | 2 cut(s) | 164, 1758 |
| HgaI | GACGC | 2 cut(s) | 750, 1223 |
| HhaI | GCGC | 2 cut(s) | 529, 1117 |
| Hin1I | GRCGYC | 1 cut(s) | 742 |
| Hin6I | GCGC | 2 cut(s) | 527, 1115 |
| HinP1I | GCGC | 2 cut(s) | 527, 1115 |
| HincII | GTYRAC | 1 cut(s) | 1093 |
| HindII | GTYRAC | 1 cut(s) | 1093 |
| HinfI | GANTC | 5 cut(s) | 264, 712, 1001, 1330, 1710 |
| HpaII | CCGG | 2 cut(s) | 164, 1758 |
| HphI | GGTGA | 2 cut(s) | 107, 1488 |
| Hpy166II | GTNNAC | 2 cut(s) | 1093, 1384 |
| Hpy188I | TCNGA | 6 cut(s) | 85, 673, 939, 958, 1006, 1734 |
| Hpy188III | TCNNGA | 4 cut(s) | 38, 543, 1028, 1516 |
| Hpy8I | GTNNAC | 2 cut(s) | 1093, 1384 |
| HpyAV | CCTTC | 2 cut(s) | 146, 619 |
| HpyCH4III | ACNGT | 4 cut(s) | 444, 721, 1126, 1264 |
| HpyCH4IV | ACGT | 1 cut(s) | 928 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 214, 343, 872, 950 |
| HpyF3I | CTNAG | 3 cut(s) | 492, 936, 957 |
| HpySE526I | ACGT | 1 cut(s) | 928 |
| Hsp92I | GRCGYC | 1 cut(s) | 742 |
| HspAI | GCGC | 2 cut(s) | 527, 1115 |
| KpnI | GGTACC | 1 cut(s) | 1125 |
| Ksp22I | TGATCA | 1 cut(s) | 1729 |
| Kzo9I | GATC | 7 cut(s) | 80, 487, 502, 832, 1642, 1688, 1729 |
| LmnI | GCTCC | 2 cut(s) | 1666, 1718 |
| Lsp1109I | GCAGC | 5 cut(s) | 38, 83, 321, 875, 887 |
| LweI | GCATC | 3 cut(s) | 217, 962, 1129 |
| MaeI | CTAG | 5 cut(s) | 738, 966, 1130, 1307, 1335 |
| MaeII | ACGT | 1 cut(s) | 928 |
| MaeIII | GTNAC | 5 cut(s) | 113, 438, 709, 826, 1494 |
| MalI | GATC | 7 cut(s) | 82, 489, 504, 834, 1644, 1690, 1731 |
| MboI | GATC | 7 cut(s) | 80, 487, 502, 832, 1642, 1688, 1729 |
| MfeI | CAATTG | 1 cut(s) | 572 |
| MflI | RGATCY | 3 cut(s) | 487, 832, 1688 |
| MhlI | GDGCHC | 1 cut(s) | 1723 |
| MlyI | GAGTC | 1 cut(s) | 706 |
| MmeI | TCCRAC | 2 cut(s) | 88, 740 |
| Mph1103I | ATGCAT | 1 cut(s) | 955 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1026 |
| MspCI | CTTAAG | 1 cut(s) | 271 |
| MspI | CCGG | 2 cut(s) | 164, 1758 |
| MunI | CAATTG | 1 cut(s) | 572 |
| Mva1269I | GAATGC | 2 cut(s) | 955, 1401 |
| MvnI | CGCG | 2 cut(s) | 529, 1117 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 214, 343, 872, 950 |
| NcoI | CCATGG | 3 cut(s) | 147, 654, 1664 |
| NdeI | CATATG | 2 cut(s) | 282, 348 |
| NdeII | GATC | 7 cut(s) | 80, 487, 502, 832, 1642, 1688, 1729 |
| NlaIV | GGNNCC | 4 cut(s) | 371, 489, 907, 1123 |
| NmuCI | GTSAC | 5 cut(s) | 113, 438, 709, 826, 1494 |
| NsiI | ATGCAT | 1 cut(s) | 955 |
| PagI | TCATGA | 1 cut(s) | 1027 |
| PctI | GAATGC | 2 cut(s) | 955, 1401 |
| PfeI | GAWTC | 4 cut(s) | 264, 1001, 1330, 1710 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1670 |
| PkrI | GCNGC | 5 cut(s) | 28, 73, 336, 865, 877 |
| Ple19I | CGATCG | 1 cut(s) | 505 |
| PleI | GAGTC | 1 cut(s) | 706 |
| PpsI | GAGTC | 1 cut(s) | 706 |
| PspEI | GGTNACC | 1 cut(s) | 1494 |
| PspFI | CCCAGC | 2 cut(s) | 208, 944 |
| PspN4I | GGNNCC | 4 cut(s) | 371, 489, 907, 1123 |
| PspPI | GGNCC | 1 cut(s) | 905 |
| PstI | CTGCAG | 1 cut(s) | 339 |
| PsuI | RGATCY | 3 cut(s) | 487, 832, 1688 |
| PvuI | CGATCG | 1 cut(s) | 505 |
| RsaI | GTAC | 5 cut(s) | 192, 931, 1123, 1128, 1589 |
| RsaNI | GTAC | 5 cut(s) | 191, 930, 1122, 1127, 1588 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1026 |
| SalI | GTCGAC | 1 cut(s) | 1091 |
| SatI | GCNGC | 5 cut(s) | 27, 72, 335, 864, 876 |
| Sau3AI | GATC | 7 cut(s) | 80, 487, 502, 832, 1642, 1688, 1729 |
| Sau96I | GGNCC | 1 cut(s) | 905 |
| SchI | GAGTC | 1 cut(s) | 706 |
| SduI | GDGCHC | 1 cut(s) | 1723 |
| SfaNI | GCATC | 3 cut(s) | 217, 962, 1129 |
| SfcI | CTRYAG | 3 cut(s) | 335, 1263, 1275 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1026 |
| SmlI | CTYRAG | 1 cut(s) | 271 |
| SmoI | CTYRAG | 1 cut(s) | 271 |
| SsiI | CCGC | 1 cut(s) | 851 |
| SspI | AATATT | 1 cut(s) | 1416 |
| SspMI | CTAG | 5 cut(s) | 738, 966, 1130, 1307, 1335 |
| StyI | CCWWGG | 4 cut(s) | 147, 654, 981, 1664 |
| TaaI | ACNGT | 4 cut(s) | 444, 721, 1126, 1264 |
| TaiI | ACGT | 1 cut(s) | 931 |
| TaqI | TCGA | 4 cut(s) | 262, 501, 1092, 1501 |
| TaqII | GACCGA | 1 cut(s) | 1722 |
| TfiI | GAWTC | 4 cut(s) | 264, 1001, 1330, 1710 |
| TscAI | CASTG | 1 cut(s) | 1267 |
| TseFI | GTSAC | 5 cut(s) | 113, 438, 709, 826, 1494 |
| TseI | GCWGC | 5 cut(s) | 26, 71, 334, 863, 875 |
| Tsp45I | GTSAC | 5 cut(s) | 113, 438, 709, 826, 1494 |
| TspDTI | ATGAA | 9 cut(s) | 408, 573, 606, 1016, 1148, 1200, 1379, 1463, 1536 |
| TspRI | CASTG | 1 cut(s) | 1267 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1670 |
| Vha464I | CTTAAG | 1 cut(s) | 271 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 185 |
| XmiI | GTMKAC | 1 cut(s) | 1092 |
| XspI | CTAG | 5 cut(s) | 738, 966, 1130, 1307, 1335 |
| Zsp2I | ATGCAT | 1 cut(s) | 955 |