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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 975 |
| Acc36I | ACCTGC | 1 cut(s) | 366 |
| AccB1I | GGYRCC | 3 cut(s) | 715, 769, 783 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 997 |
| AccI | GTMKAC | 1 cut(s) | 1004 |
| AccII | CGCG | 1 cut(s) | 1023 |
| AciI | CCGC | 4 cut(s) | 781, 863, 963, 1021 |
| AclWI | GGATC | 1 cut(s) | 608 |
| AcoI | YGGCCR | 3 cut(s) | 296, 700, 834 |
| AcsI | RAATTY | 2 cut(s) | 26, 456 |
| AfaI | GTAC | 1 cut(s) | 192 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 997 |
| AflIII | ACRYGT | 2 cut(s) | 193, 516 |
| AgeI | ACCGGT | 1 cut(s) | 827 |
| AgsI | TTSAA | 4 cut(s) | 50, 116, 577, 874 |
| AjiI | CACGTC | 1 cut(s) | 196 |
| AjnI | CCWGG | 1 cut(s) | 697 |
| AluBI | AGCT | 6 cut(s) | 46, 227, 479, 558, 662, 877 |
| AluI | AGCT | 6 cut(s) | 46, 227, 479, 558, 662, 877 |
| Alw21I | GWGCWC | 3 cut(s) | 560, 634, 664 |
| Alw26I | GTCTC | 2 cut(s) | 137, 202 |
| Alw44I | GTGCAC | 1 cut(s) | 630 |
| AlwI | GGATC | 1 cut(s) | 608 |
| AoxI | GGCC | 4 cut(s) | 296, 402, 700, 834 |
| ApaLI | GTGCAC | 1 cut(s) | 630 |
| ApeKI | GCWGC | 4 cut(s) | 323, 479, 877, 1084 |
| ApoI | RAATTY | 2 cut(s) | 26, 456 |
| AsiGI | ACCGGT | 1 cut(s) | 827 |
| AspLEI | GCGC | 1 cut(s) | 1025 |
| AspS9I | GGNCC | 2 cut(s) | 402, 426 |
| AsuHPI | GGTGA | 1 cut(s) | 79 |
| AvaII | GGWCC | 1 cut(s) | 426 |
| BaeGI | GKGCMC | 3 cut(s) | 391, 634, 788 |
| BalI | TGGCCA | 2 cut(s) | 298, 702 |
| BanI | GGYRCC | 3 cut(s) | 715, 769, 783 |
| BanII | GRGCYC | 3 cut(s) | 560, 664, 954 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 216, 248 |
| BbsI | GAAGAC | 1 cut(s) | 973 |
| Bbv12I | GWGCWC | 3 cut(s) | 560, 634, 664 |
| BbvI | GCAGC | 4 cut(s) | 335, 466, 864, 1071 |
| BccI | CCATC | 7 cut(s) | 416, 440, 816, 901, 953, 1045, 1129 |
| BceAI | ACGGC | 2 cut(s) | 703, 1127 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1066, 1100 |
| BciT130I | CCWGG | 1 cut(s) | 699 |
| BclI | TGATCA | 1 cut(s) | 619 |
| BcoDI | GTCTC | 2 cut(s) | 137, 202 |
| BfaI | CTAG | 3 cut(s) | 308, 374, 1148 |
| BfmI | CTRYAG | 1 cut(s) | 878 |
| BfuAI | ACCTGC | 1 cut(s) | 366 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 778 |
| BisI | GCNGC | 4 cut(s) | 324, 480, 878, 1085 |
| BlsI | GCNGC | 4 cut(s) | 325, 481, 879, 1086 |
| Bme1390I | CCNGG | 1 cut(s) | 699 |
| Bme18I | GGWCC | 1 cut(s) | 426 |
| BmgBI | CACGTC | 1 cut(s) | 196 |
| BmgT120I | GGNCC | 2 cut(s) | 402, 426 |
| BmiI | GGNNCC | 8 cut(s) | 427, 683, 717, 771, 785, 805, 953, 989 |
| BmrFI | CCNGG | 1 cut(s) | 699 |
| BmsI | GCATC | 1 cut(s) | 931 |
| BpiI | GAAGAC | 1 cut(s) | 973 |
| BpuEI | CTTGAG | 2 cut(s) | 161, 712 |
| BsaBI | GATNNNNATC | 1 cut(s) | 618 |
| BsaI | GGTCTC | 1 cut(s) | 137 |
| BsaJI | CCNNGG | 1 cut(s) | 163 |
| BsaWI | WCCGGW | 1 cut(s) | 827 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 354, 384, 788, 818 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 997 |
| Bse118I | RCCGGY | 1 cut(s) | 827 |
| Bse3DI | GCAATG | 1 cut(s) | 921 |
| Bse8I | GATNNNNATC | 1 cut(s) | 618 |
| BseBI | CCWGG | 1 cut(s) | 699 |
| BseDI | CCNNGG | 1 cut(s) | 163 |
| BseGI | GGATG | 3 cut(s) | 277, 432, 808 |
| BseJI | GATNNNNATC | 1 cut(s) | 618 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 997 |
| BseMI | GCAATG | 1 cut(s) | 921 |
| BseMII | CTCAG | 1 cut(s) | 917 |
| BseSI | GKGCMC | 3 cut(s) | 391, 634, 788 |
| BseXI | GCAGC | 4 cut(s) | 335, 466, 864, 1071 |
| Bsh1236I | CGCG | 1 cut(s) | 1023 |
| BshFI | GGCC | 4 cut(s) | 298, 404, 702, 836 |
| BshNI | GGYRCC | 3 cut(s) | 715, 769, 783 |
| BshTI | ACCGGT | 1 cut(s) | 827 |
| BsiHKAI | GWGCWC | 3 cut(s) | 560, 634, 664 |
| BsiSI | CCGG | 4 cut(s) | 39, 778, 828, 837 |
| BslFI | GGGAC | 2 cut(s) | 295, 439 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 997 |
| BsmAI | GTCTC | 2 cut(s) | 137, 202 |
| BsmBI | CGTCTC | 1 cut(s) | 202 |
| BsmFI | GGGAC | 2 cut(s) | 295, 439 |
| BsnI | GGCC | 4 cut(s) | 298, 404, 702, 836 |
| Bso31I | GGTCTC | 1 cut(s) | 137 |
| Bsp1286I | GDGCHC | 6 cut(s) | 391, 560, 634, 664, 788, 954 |
| Bsp1407I | TGTACA | 1 cut(s) | 190 |
| Bsp143I | GATC | 3 cut(s) | 79, 613, 619 |
| Bsp19I | CCATGG | 1 cut(s) | 163 |
| BspACI | CCGC | 4 cut(s) | 781, 863, 963, 1021 |
| BspANI | GGCC | 4 cut(s) | 298, 404, 702, 836 |
| BspCNI | CTCAG | 1 cut(s) | 916 |
| BspFNI | CGCG | 1 cut(s) | 1023 |
| BspHI | TCATGA | 3 cut(s) | 220, 552, 565 |
| BspLI | GGNNCC | 8 cut(s) | 427, 683, 717, 771, 785, 805, 953, 989 |
| BspMAI | CTGCAG | 1 cut(s) | 882 |
| BspMI | ACCTGC | 1 cut(s) | 366 |
| BspPI | GGATC | 1 cut(s) | 608 |
| BspT107I | GGYRCC | 3 cut(s) | 715, 769, 783 |
| BspTNI | GGTCTC | 1 cut(s) | 137 |
| BsrDI | GCAATG | 1 cut(s) | 921 |
| BsrFI | RCCGGY | 1 cut(s) | 827 |
| BsrGI | TGTACA | 1 cut(s) | 190 |
| BssAI | RCCGGY | 1 cut(s) | 827 |
| BssECI | CCNNGG | 1 cut(s) | 163 |
| BssMI | GATC | 3 cut(s) | 79, 613, 619 |
| BssT1I | CCWWGG | 1 cut(s) | 163 |
| Bst2UI | CCWGG | 1 cut(s) | 699 |
| Bst4CI | ACNGT | 3 cut(s) | 265, 800, 1014 |
| Bst6I | CTCTTC | 1 cut(s) | 572 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 358 |
| BstAUI | TGTACA | 1 cut(s) | 190 |
| BstDEI | CTNAG | 3 cut(s) | 585, 903, 974 |
| BstDSI | CCRYGG | 1 cut(s) | 163 |
| BstF5I | GGATG | 3 cut(s) | 277, 432, 808 |
| BstFNI | CGCG | 1 cut(s) | 1023 |
| BstHHI | GCGC | 1 cut(s) | 1025 |
| BstKTI | GATC | 3 cut(s) | 82, 616, 622 |
| BstMAI | GTCTC | 2 cut(s) | 137, 202 |
| BstMBI | GATC | 3 cut(s) | 79, 613, 619 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 358, 386, 778 |
| BstNI | CCWGG | 1 cut(s) | 699 |
| BstNSI | RCATGY | 1 cut(s) | 520 |
| BstSCI | CCNGG | 1 cut(s) | 697 |
| BstSFI | CTRYAG | 1 cut(s) | 878 |
| BstSLI | GKGCMC | 3 cut(s) | 391, 634, 788 |
| BstUI | CGCG | 1 cut(s) | 1023 |
| BstV1I | GCAGC | 4 cut(s) | 335, 466, 864, 1071 |
| BstV2I | GAAGAC | 1 cut(s) | 973 |
| BsuRI | GGCC | 4 cut(s) | 298, 404, 702, 836 |
| BtgI | CCRYGG | 1 cut(s) | 163 |
| BtrI | CACGTC | 1 cut(s) | 196 |
| BtsCI | GGATG | 3 cut(s) | 277, 432, 808 |
| BtsI | GCAGTG | 1 cut(s) | 887 |
| BtsIMutI | CAGTG | 2 cut(s) | 792, 887 |
| BveI | ACCTGC | 1 cut(s) | 366 |
| CciI | TCATGA | 3 cut(s) | 220, 552, 565 |
| CfoI | GCGC | 1 cut(s) | 1025 |
| Cfr10I | RCCGGY | 1 cut(s) | 827 |
| Cfr13I | GGNCC | 2 cut(s) | 402, 426 |
| Csp6I | GTAC | 1 cut(s) | 191 |
| CspAI | ACCGGT | 1 cut(s) | 827 |
| CviAII | CATG | 8 cut(s) | 164, 221, 410, 508, 517, 553, 566, 598 |
| CviQI | GTAC | 1 cut(s) | 191 |
| DdeI | CTNAG | 3 cut(s) | 585, 903, 974 |
| DpnI | GATC | 3 cut(s) | 81, 615, 621 |
| DpnII | GATC | 3 cut(s) | 79, 613, 619 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 975 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 975 |
| EaeI | YGGCCR | 3 cut(s) | 296, 700, 834 |
| Eam1104I | CTCTTC | 1 cut(s) | 572 |
| EarI | CTCTTC | 1 cut(s) | 572 |
| EciI | GGCGGA | 1 cut(s) | 978 |
| Ecl136II | GAGCTC | 2 cut(s) | 558, 662 |
| Eco130I | CCWWGG | 1 cut(s) | 163 |
| Eco24I | GRGCYC | 3 cut(s) | 560, 664, 954 |
| Eco31I | GGTCTC | 1 cut(s) | 137 |
| Eco47I | GGWCC | 1 cut(s) | 426 |
| Eco53kI | GAGCTC | 2 cut(s) | 558, 662 |
| EcoICRI | GAGCTC | 2 cut(s) | 558, 662 |
| EcoRI | GAATTC | 1 cut(s) | 26 |
| EcoRII | CCWGG | 1 cut(s) | 697 |
| EcoT14I | CCWWGG | 1 cut(s) | 163 |
| EcoT38I | GRGCYC | 3 cut(s) | 560, 664, 954 |
| ErhI | CCWWGG | 1 cut(s) | 163 |
| Esp3I | CGTCTC | 1 cut(s) | 202 |
| FaeI | CATG | 8 cut(s) | 167, 224, 413, 511, 520, 556, 569, 601 |
| FaqI | GGGAC | 2 cut(s) | 295, 439 |
| FatI | CATG | 8 cut(s) | 163, 220, 409, 507, 516, 552, 565, 597 |
| FauI | CCCGC | 1 cut(s) | 856 |
| FbaI | TGATCA | 1 cut(s) | 619 |
| FblI | GTMKAC | 1 cut(s) | 1004 |
| Fnu4HI | GCNGC | 4 cut(s) | 324, 480, 878, 1085 |
| FokI | GGATG | 3 cut(s) | 284, 419, 795 |
| FriOI | GRGCYC | 3 cut(s) | 560, 664, 954 |
| Fsp4HI | GCNGC | 4 cut(s) | 324, 480, 878, 1085 |
| FspBI | CTAG | 3 cut(s) | 308, 374, 1148 |
| GlaI | GCGC | 1 cut(s) | 1024 |
| GluI | GCNGC | 4 cut(s) | 324, 480, 878, 1085 |
| HaeIII | GGCC | 4 cut(s) | 298, 404, 702, 836 |
| HapII | CCGG | 4 cut(s) | 39, 778, 828, 837 |
| HhaI | GCGC | 1 cut(s) | 1025 |
| Hin1II | CATG | 8 cut(s) | 167, 224, 413, 511, 520, 556, 569, 601 |
| Hin6I | GCGC | 1 cut(s) | 1023 |
| HinP1I | GCGC | 1 cut(s) | 1023 |
| HincII | GTYRAC | 2 cut(s) | 136, 1005 |
| HindII | GTYRAC | 2 cut(s) | 136, 1005 |
| HindIII | AAGCTT | 1 cut(s) | 225 |
| HinfI | GANTC | 1 cut(s) | 1001 |
| HpaII | CCGG | 4 cut(s) | 39, 778, 828, 837 |
| HphI | GGTGA | 1 cut(s) | 79 |
| Hpy166II | GTNNAC | 4 cut(s) | 136, 193, 632, 1005 |
| Hpy188I | TCNGA | 2 cut(s) | 210, 906 |
| Hpy188III | TCNNGA | 8 cut(s) | 50, 221, 334, 531, 553, 566, 617, 1075 |
| Hpy8I | GTNNAC | 4 cut(s) | 136, 193, 632, 1005 |
| Hpy99I | CGWCG | 2 cut(s) | 1009, 1081 |
| HpyAV | CCTTC | 3 cut(s) | 58, 465, 572 |
| HpyCH4III | ACNGT | 3 cut(s) | 265, 800, 1014 |
| HpyCH4IV | ACGT | 2 cut(s) | 195, 212 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 358, 386, 778 |
| HpyF3I | CTNAG | 3 cut(s) | 585, 903, 974 |
| HpySE526I | ACGT | 2 cut(s) | 195, 212 |
| Hsp92II | CATG | 8 cut(s) | 167, 224, 413, 511, 520, 556, 569, 601 |
| HspAI | GCGC | 1 cut(s) | 1023 |
| Ksp22I | TGATCA | 1 cut(s) | 619 |
| Kzo9I | GATC | 3 cut(s) | 79, 613, 619 |
| LmnI | GCTCC | 3 cut(s) | 563, 809, 957 |
| Lsp1109I | GCAGC | 4 cut(s) | 335, 466, 864, 1071 |
| LweI | GCATC | 1 cut(s) | 931 |
| MaeI | CTAG | 3 cut(s) | 308, 374, 1148 |
| MaeII | ACGT | 2 cut(s) | 195, 212 |
| MaeIII | GTNAC | 2 cut(s) | 259, 494 |
| MalI | GATC | 3 cut(s) | 81, 615, 621 |
| MboI | GATC | 3 cut(s) | 79, 613, 619 |
| MboII | GAAGA | 3 cut(s) | 589, 978, 1063 |
| MhlI | GDGCHC | 6 cut(s) | 391, 560, 634, 664, 788, 954 |
| MlsI | TGGCCA | 2 cut(s) | 298, 702 |
| MluCI | AATT | 4 cut(s) | 26, 456, 737, 1171 |
| MluNI | TGGCCA | 2 cut(s) | 298, 702 |
| MlyI | GAGTC | 1 cut(s) | 1010 |
| MmeI | TCCRAC | 1 cut(s) | 501 |
| MnlI | CCTC | 8 cut(s) | 109, 175, 451, 527, 573, 754, 822, 1112 |
| Mox20I | TGGCCA | 2 cut(s) | 298, 702 |
| MscI | TGGCCA | 2 cut(s) | 298, 702 |
| MseI | TTAA | 4 cut(s) | 21, 120, 267, 824 |
| MslI | CAYNNNNRTG | 1 cut(s) | 627 |
| Msp20I | TGGCCA | 2 cut(s) | 298, 702 |
| MspI | CCGG | 4 cut(s) | 39, 778, 828, 837 |
| MspR9I | CCNGG | 1 cut(s) | 699 |
| MvaI | CCWGG | 1 cut(s) | 699 |
| MvnI | CGCG | 1 cut(s) | 1023 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 358, 386, 778 |
| NcoI | CCATGG | 1 cut(s) | 163 |
| NdeII | GATC | 3 cut(s) | 79, 613, 619 |
| NlaIII | CATG | 8 cut(s) | 167, 224, 413, 511, 520, 556, 569, 601 |
| NlaIV | GGNNCC | 8 cut(s) | 427, 683, 717, 771, 785, 805, 953, 989 |
| NmuCI | GTSAC | 2 cut(s) | 259, 494 |
| NspI | RCATGY | 1 cut(s) | 520 |
| PagI | TCATGA | 3 cut(s) | 220, 552, 565 |
| PciI | ACATGT | 1 cut(s) | 516 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 997 |
| PinAI | ACCGGT | 1 cut(s) | 827 |
| PkrI | GCNGC | 4 cut(s) | 325, 481, 879, 1086 |
| PleI | GAGTC | 1 cut(s) | 1009 |
| PpsI | GAGTC | 1 cut(s) | 1009 |
| PscI | ACATGT | 1 cut(s) | 516 |
| Psp124BI | GAGCTC | 2 cut(s) | 560, 664 |
| Psp6I | CCWGG | 1 cut(s) | 697 |
| PspGI | CCWGG | 1 cut(s) | 697 |
| PspN4I | GGNNCC | 8 cut(s) | 427, 683, 717, 771, 785, 805, 953, 989 |
| PspPI | GGNCC | 2 cut(s) | 402, 426 |
| PstI | CTGCAG | 1 cut(s) | 882 |
| RsaI | GTAC | 1 cut(s) | 192 |
| RsaNI | GTAC | 1 cut(s) | 191 |
| RseI | CAYNNNNRTG | 1 cut(s) | 627 |
| SacI | GAGCTC | 2 cut(s) | 560, 664 |
| SalI | GTCGAC | 1 cut(s) | 1003 |
| SaqAI | TTAA | 4 cut(s) | 21, 120, 267, 824 |
| SatI | GCNGC | 4 cut(s) | 324, 480, 878, 1085 |
| Sau3AI | GATC | 3 cut(s) | 79, 613, 619 |
| Sau96I | GGNCC | 2 cut(s) | 402, 426 |
| SchI | GAGTC | 1 cut(s) | 1010 |
| ScrFI | CCNGG | 1 cut(s) | 699 |
| SduI | GDGCHC | 6 cut(s) | 391, 560, 634, 664, 788, 954 |
| SfaNI | GCATC | 1 cut(s) | 931 |
| SfcI | CTRYAG | 1 cut(s) | 878 |
| SinI | GGWCC | 1 cut(s) | 426 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 627 |
| SmlI | CTYRAG | 2 cut(s) | 140, 727 |
| SmoI | CTYRAG | 2 cut(s) | 140, 727 |
| Sse9I | AATT | 4 cut(s) | 26, 456, 737, 1171 |
| SsiI | CCGC | 4 cut(s) | 781, 863, 963, 1021 |
| SspMI | CTAG | 3 cut(s) | 308, 374, 1148 |
| SstI | GAGCTC | 2 cut(s) | 560, 664 |
| StyD4I | CCNGG | 1 cut(s) | 697 |
| StyI | CCWWGG | 1 cut(s) | 163 |
| TaaI | ACNGT | 3 cut(s) | 265, 800, 1014 |
| TaiI | ACGT | 2 cut(s) | 198, 215 |
| TaqI | TCGA | 5 cut(s) | 469, 612, 979, 1004, 1076 |
| TaqII | GACCGA | 1 cut(s) | 654 |
| TasI | AATT | 4 cut(s) | 26, 456, 737, 1171 |
| TatI | WGTACW | 1 cut(s) | 190 |
| Tru1I | TTAA | 4 cut(s) | 21, 120, 267, 824 |
| Tru9I | TTAA | 4 cut(s) | 21, 120, 267, 824 |
| TscAI | CASTG | 2 cut(s) | 799, 887 |
| TseFI | GTSAC | 2 cut(s) | 259, 494 |
| TseI | GCWGC | 4 cut(s) | 323, 479, 877, 1084 |
| Tsp45I | GTSAC | 2 cut(s) | 259, 494 |
| TspDTI | ATGAA | 6 cut(s) | 162, 209, 237, 426, 541, 554 |
| TspRI | CASTG | 2 cut(s) | 799, 887 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 997 |
| VneI | GTGCAC | 1 cut(s) | 630 |
| VpaK11BI | GGWCC | 1 cut(s) | 426 |
| XapI | RAATTY | 2 cut(s) | 26, 456 |
| XceI | RCATGY | 1 cut(s) | 520 |
| XmiI | GTMKAC | 1 cut(s) | 1004 |
| XspI | CTAG | 3 cut(s) | 308, 374, 1148 |