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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1598 |
| Acc36I | ACCTGC | 1 cut(s) | 1739 |
| Acc65I | GGTACC | 2 cut(s) | 2482, 2712 |
| AccB1I | GGYRCC | 4 cut(s) | 778, 2482, 2712, 2866 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1730 |
| AccI | GTMKAC | 1 cut(s) | 794 |
| AciI | CCGC | 9 cut(s) | 101, 319, 699, 1028, 1174, 1207, 1818, 2041, 2953 |
| AclWI | GGATC | 4 cut(s) | 333, 563, 2933, 2946 |
| AcoI | YGGCCR | 3 cut(s) | 1504, 1927, 2408 |
| AcsI | RAATTY | 5 cut(s) | 107, 260, 1124, 1373, 3156 |
| AcuI | CTGAAG | 7 cut(s) | 84, 114, 831, 938, 1425, 2088, 2525 |
| AcvI | CACGTG | 1 cut(s) | 1355 |
| AcyI | GRCGYC | 1 cut(s) | 779 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 185, 1730, 1793, 2776, 2949 |
| AflIII | ACRYGT | 3 cut(s) | 1329, 1354, 2305 |
| AjnI | CCWGG | 3 cut(s) | 1605, 1951, 2848 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2061, 2093 |
| AleI | CACNNNNGTG | 3 cut(s) | 237, 518, 2823 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 823, 855, 997, 1029 |
| Alw21I | GWGCWC | 3 cut(s) | 193, 285, 2571 |
| Alw26I | GTCTC | 7 cut(s) | 600, 1137, 1331, 1640, 2090, 2314, 2443 |
| AlwI | GGATC | 4 cut(s) | 333, 563, 2933, 2946 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 2099 |
| AoxI | GGCC | 9 cut(s) | 59, 176, 913, 1504, 1686, 1909, 1927, 2408, 3029 |
| ApeKI | GCWGC | 4 cut(s) | 818, 1106, 1349, 2111 |
| ApoI | RAATTY | 5 cut(s) | 107, 260, 1124, 1373, 3156 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 203, 235, 1899, 1931 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 545 |
| Asp718I | GGTACC | 2 cut(s) | 2482, 2712 |
| AspLEI | GCGC | 1 cut(s) | 781 |
| AspS9I | GGNCC | 3 cut(s) | 913, 1686, 2293 |
| AsuHPI | GGTGA | 6 cut(s) | 650, 1544, 1969, 2011, 2206, 2433 |
| AsuII | TTCGAA | 1 cut(s) | 46 |
| AsuNHI | GCTAGC | 1 cut(s) | 1802 |
| AvaII | GGWCC | 1 cut(s) | 2293 |
| AxyI | CCTNAGG | 1 cut(s) | 2607 |
| BalI | TGGCCA | 1 cut(s) | 1929 |
| BamHI | GGATCC | 1 cut(s) | 2938 |
| BanI | GGYRCC | 4 cut(s) | 778, 2482, 2712, 2866 |
| BanII | GRGCYC | 1 cut(s) | 2571 |
| BauI | CACGAG | 1 cut(s) | 159 |
| BbrPI | CACGTG | 1 cut(s) | 1355 |
| BbsI | GAAGAC | 1 cut(s) | 138 |
| Bbv12I | GWGCWC | 3 cut(s) | 193, 285, 2571 |
| BbvI | GCAGC | 4 cut(s) | 805, 1093, 1336, 2123 |
| BccI | CCATC | 7 cut(s) | 160, 417, 674, 691, 790, 1110, 1787 |
| BciT130I | CCWGG | 3 cut(s) | 1607, 1953, 2850 |
| BciVI | GTATCC | 4 cut(s) | 15, 722, 1200, 2612 |
| BcoDI | GTCTC | 7 cut(s) | 600, 1137, 1331, 1640, 2090, 2314, 2443 |
| BfaI | CTAG | 7 cut(s) | 57, 1449, 1560, 1764, 1769, 1803, 2829 |
| BfmI | CTRYAG | 3 cut(s) | 610, 2232, 2961 |
| BfoI | RGCGCY | 1 cut(s) | 782 |
| BfuAI | ACCTGC | 1 cut(s) | 1739 |
| BfuI | GTATCC | 4 cut(s) | 15, 722, 1200, 2612 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 1256, 1271 |
| BglII | AGATCT | 1 cut(s) | 543 |
| BisI | GCNGC | 6 cut(s) | 819, 1029, 1107, 1174, 1350, 2112 |
| BlpI | GCTNAGC | 1 cut(s) | 1623 |
| BlsI | GCNGC | 6 cut(s) | 820, 1030, 1108, 1175, 1351, 2113 |
| BmcAI | AGTACT | 1 cut(s) | 1617 |
| Bme1390I | CCNGG | 3 cut(s) | 1607, 1953, 2850 |
| Bme18I | GGWCC | 1 cut(s) | 2293 |
| BmgT120I | GGNCC | 3 cut(s) | 913, 1686, 2293 |
| BmiI | GGNNCC | 8 cut(s) | 596, 780, 2294, 2484, 2714, 2868, 2910, 2940 |
| BmrFI | CCNGG | 3 cut(s) | 1607, 1953, 2850 |
| BmrI | ACTGGG | 3 cut(s) | 379, 1902, 2694 |
| BmsI | GCATC | 5 cut(s) | 669, 830, 875, 2134, 2503 |
| BmtI | GCTAGC | 1 cut(s) | 1806 |
| BmuI | ACTGGG | 3 cut(s) | 379, 1902, 2694 |
| BpiI | GAAGAC | 1 cut(s) | 138 |
| BpmI | CTGGAG | 2 cut(s) | 762, 1461 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1623 |
| Bpu14I | TTCGAA | 1 cut(s) | 46 |
| BpuEI | CTTGAG | 1 cut(s) | 2609 |
| BsaAI | YACGTR | 3 cut(s) | 1355, 2306, 3124 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1598 |
| BsaHI | GRCGYC | 1 cut(s) | 779 |
| BsaJI | CCNNGG | 6 cut(s) | 800, 1952, 2115, 2388, 2967, 3061 |
| BsaWI | WCCGGW | 2 cut(s) | 2191, 2911 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 997, 1027, 1050, 1080 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 185, 1730, 1793, 2776, 2949 |
| Bse118I | RCCGGY | 3 cut(s) | 1255, 1270, 1523 |
| Bse1I | ACTGG | 8 cut(s) | 374, 1444, 1623, 1724, 1897, 2139, 2533, 2689 |
| Bse21I | CCTNAGG | 1 cut(s) | 2607 |
| Bse3DI | GCAATG | 4 cut(s) | 451, 951, 1998, 2250 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1598 |
| BseBI | CCWGG | 3 cut(s) | 1607, 1953, 2850 |
| BseDI | CCNNGG | 6 cut(s) | 800, 1952, 2115, 2388, 2967, 3061 |
| BseGI | GGATG | 5 cut(s) | 405, 782, 1185, 1315, 1779 |
| BseJI | GATNNNNATC | 1 cut(s) | 1598 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 185, 1730, 1793, 2776, 2949 |
| BseMI | GCAATG | 4 cut(s) | 451, 951, 1998, 2250 |
| BseMII | CTCAG | 4 cut(s) | 69, 1614, 2471, 2598 |
| BseNI | ACTGG | 8 cut(s) | 374, 1444, 1623, 1724, 1897, 2139, 2533, 2689 |
| BseRI | GAGGAG | 3 cut(s) | 761, 911, 2050 |
| BseXI | GCAGC | 4 cut(s) | 805, 1093, 1336, 2123 |
| BsgI | GTGCAG | 2 cut(s) | 1335, 2580 |
| BshFI | GGCC | 9 cut(s) | 61, 178, 915, 1506, 1688, 1911, 1929, 2410, 3031 |
| BshNI | GGYRCC | 4 cut(s) | 778, 2482, 2712, 2866 |
| BsiHKAI | GWGCWC | 3 cut(s) | 193, 285, 2571 |
| BsiSI | CCGG | 7 cut(s) | 1256, 1271, 1286, 1524, 2192, 2411, 2912 |
| BslFI | GGGAC | 4 cut(s) | 1024, 1189, 1755, 2306 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 185, 1730, 1793, 2776, 2949 |
| BsmAI | GTCTC | 7 cut(s) | 600, 1137, 1331, 1640, 2090, 2314, 2443 |
| BsmFI | GGGAC | 4 cut(s) | 1024, 1189, 1755, 2306 |
| BsnI | GGCC | 9 cut(s) | 61, 178, 915, 1506, 1688, 1911, 1929, 2410, 3031 |
| Bsp119I | TTCGAA | 1 cut(s) | 46 |
| Bsp1286I | GDGCHC | 3 cut(s) | 193, 285, 2571 |
| Bsp143I | GATC | 8 cut(s) | 325, 543, 568, 1638, 1822, 2679, 2881, 2938 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1623 |
| Bsp19I | CCATGG | 1 cut(s) | 2388 |
| BspACI | CCGC | 9 cut(s) | 101, 319, 699, 1028, 1174, 1207, 1818, 2041, 2953 |
| BspANI | GGCC | 9 cut(s) | 61, 178, 915, 1506, 1688, 1911, 1929, 2410, 3031 |
| BspCNI | CTCAG | 4 cut(s) | 70, 1615, 2470, 2599 |
| BspHI | TCATGA | 1 cut(s) | 892 |
| BspLI | GGNNCC | 8 cut(s) | 596, 780, 2294, 2484, 2714, 2868, 2910, 2940 |
| BspMI | ACCTGC | 1 cut(s) | 1739 |
| BspOI | GCTAGC | 1 cut(s) | 1806 |
| BspPI | GGATC | 4 cut(s) | 333, 563, 2933, 2946 |
| BspT104I | TTCGAA | 1 cut(s) | 46 |
| BspT107I | GGYRCC | 4 cut(s) | 778, 2482, 2712, 2866 |
| BsrDI | GCAATG | 4 cut(s) | 451, 951, 1998, 2250 |
| BsrFI | RCCGGY | 3 cut(s) | 1255, 1270, 1523 |
| BsrI | ACTGG | 8 cut(s) | 374, 1444, 1623, 1724, 1897, 2139, 2533, 2689 |
| BssAI | RCCGGY | 3 cut(s) | 1255, 1270, 1523 |
| BssECI | CCNNGG | 6 cut(s) | 800, 1952, 2115, 2388, 2967, 3061 |
| BssMI | GATC | 8 cut(s) | 325, 543, 568, 1638, 1822, 2679, 2881, 2938 |
| BssNI | GRCGYC | 1 cut(s) | 779 |
| BssSI | CACGAG | 1 cut(s) | 159 |
| BssT1I | CCWWGG | 3 cut(s) | 800, 2388, 3061 |
| Bst2BI | CACGAG | 1 cut(s) | 159 |
| Bst2UI | CCWGG | 3 cut(s) | 1607, 1953, 2850 |
| Bst4CI | ACNGT | 7 cut(s) | 169, 614, 673, 1716, 2377, 2800, 2902 |
| Bst6I | CTCTTC | 5 cut(s) | 944, 977, 1284, 1401, 2459 |
| BstACI | GRCGYC | 1 cut(s) | 779 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 2522 |
| BstBAI | YACGTR | 3 cut(s) | 1355, 2306, 3124 |
| BstBI | TTCGAA | 1 cut(s) | 46 |
| BstDEI | CTNAG | 6 cut(s) | 78, 1454, 1623, 2457, 2607, 2878 |
| BstDSI | CCRYGG | 1 cut(s) | 2388 |
| BstEII | GGTNACC | 1 cut(s) | 2955 |
| BstF5I | GGATG | 5 cut(s) | 405, 782, 1185, 1315, 1779 |
| BstH2I | RGCGCY | 1 cut(s) | 782 |
| BstHHI | GCGC | 1 cut(s) | 781 |
| BstKTI | GATC | 8 cut(s) | 328, 546, 571, 1641, 1825, 2682, 2884, 2941 |
| BstMAI | GTCTC | 7 cut(s) | 600, 1137, 1331, 1640, 2090, 2314, 2443 |
| BstMBI | GATC | 8 cut(s) | 325, 543, 568, 1638, 1822, 2679, 2881, 2938 |
| BstNI | CCWGG | 3 cut(s) | 1607, 1953, 2850 |
| BstNSI | RCATGY | 3 cut(s) | 736, 1333, 2554 |
| BstPI | GGTNACC | 1 cut(s) | 2955 |
| BstSCI | CCNGG | 3 cut(s) | 1605, 1951, 2848 |
| BstSFI | CTRYAG | 3 cut(s) | 610, 2232, 2961 |
| BstSNI | TACGTA | 1 cut(s) | 3124 |
| BstV1I | GCAGC | 4 cut(s) | 805, 1093, 1336, 2123 |
| BstV2I | GAAGAC | 1 cut(s) | 138 |
| BstX2I | RGATCY | 2 cut(s) | 543, 2938 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 2746 |
| BstYI | RGATCY | 2 cut(s) | 543, 2938 |
| Bsu36I | CCTNAGG | 1 cut(s) | 2607 |
| BsuI | GTATCC | 4 cut(s) | 15, 722, 1200, 2612 |
| BsuRI | GGCC | 9 cut(s) | 61, 178, 915, 1506, 1688, 1911, 1929, 2410, 3031 |
| BtgI | CCRYGG | 1 cut(s) | 2388 |
| BtgZI | GCGATG | 1 cut(s) | 2376 |
| BtsCI | GGATG | 5 cut(s) | 405, 782, 1185, 1315, 1779 |
| BtsI | GCAGTG | 1 cut(s) | 861 |
| BtsIMutI | CAGTG | 7 cut(s) | 165, 619, 678, 861, 1890, 2146, 2682 |
| BveI | ACCTGC | 1 cut(s) | 1739 |
| CaiI | CAGNNNCTG | 1 cut(s) | 2099 |
| CciI | TCATGA | 1 cut(s) | 892 |
| CfoI | GCGC | 1 cut(s) | 781 |
| Cfr10I | RCCGGY | 3 cut(s) | 1255, 1270, 1523 |
| Cfr13I | GGNCC | 3 cut(s) | 913, 1686, 2293 |
| CspCI | CAANNNNNGTGG | 1 cut(s) | 3133 |
| DdeI | CTNAG | 6 cut(s) | 78, 1454, 1623, 2457, 2607, 2878 |
| DinI | GGCGCC | 1 cut(s) | 780 |
| DpnI | GATC | 8 cut(s) | 327, 545, 570, 1640, 1824, 2681, 2883, 2940 |
| DpnII | GATC | 8 cut(s) | 325, 543, 568, 1638, 1822, 2679, 2881, 2938 |
| EaeI | YGGCCR | 3 cut(s) | 1504, 1927, 2408 |
| Eam1104I | CTCTTC | 5 cut(s) | 944, 977, 1284, 1401, 2459 |
| EarI | CTCTTC | 5 cut(s) | 944, 977, 1284, 1401, 2459 |
| Ecl136II | GAGCTC | 1 cut(s) | 2569 |
| Eco105I | TACGTA | 1 cut(s) | 3124 |
| Eco130I | CCWWGG | 3 cut(s) | 800, 2388, 3061 |
| Eco147I | AGGCCT | 2 cut(s) | 61, 178 |
| Eco24I | GRGCYC | 1 cut(s) | 2571 |
| Eco47I | GGWCC | 1 cut(s) | 2293 |
| Eco53kI | GAGCTC | 1 cut(s) | 2569 |
| Eco57I | CTGAAG | 7 cut(s) | 84, 114, 831, 938, 1425, 2088, 2525 |
| Eco72I | CACGTG | 1 cut(s) | 1355 |
| Eco81I | CCTNAGG | 1 cut(s) | 2607 |
| Eco91I | GGTNACC | 1 cut(s) | 2955 |
| EcoICRI | GAGCTC | 1 cut(s) | 2569 |
| EcoO109I | RGGNCCY | 1 cut(s) | 913 |
| EcoO65I | GGTNACC | 1 cut(s) | 2955 |
| EcoRI | GAATTC | 2 cut(s) | 260, 1124 |
| EcoRII | CCWGG | 3 cut(s) | 1605, 1951, 2848 |
| EcoT14I | CCWWGG | 3 cut(s) | 800, 2388, 3061 |
| EcoT22I | ATGCAT | 3 cut(s) | 1681, 1845, 2552 |
| EcoT38I | GRGCYC | 1 cut(s) | 2571 |
| EgeI | GGCGCC | 1 cut(s) | 780 |
| EheI | GGCGCC | 1 cut(s) | 780 |
| ErhI | CCWWGG | 3 cut(s) | 800, 2388, 3061 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1952, 1984, 2112, 2144 |
| FaqI | GGGAC | 4 cut(s) | 1024, 1189, 1755, 2306 |
| FauI | CCCGC | 1 cut(s) | 1214 |
| FauNDI | CATATG | 1 cut(s) | 1018 |
| FblI | GTMKAC | 1 cut(s) | 794 |
| Fnu4HI | GCNGC | 6 cut(s) | 819, 1029, 1107, 1174, 1350, 2112 |
| FokI | GGATG | 5 cut(s) | 392, 769, 1192, 1322, 1766 |
| FriOI | GRGCYC | 1 cut(s) | 2571 |
| Fsp4HI | GCNGC | 6 cut(s) | 819, 1029, 1107, 1174, 1350, 2112 |
| FspBI | CTAG | 7 cut(s) | 57, 1449, 1560, 1764, 1769, 1803, 2829 |
| GlaI | GCGC | 1 cut(s) | 780 |
| GluI | GCNGC | 6 cut(s) | 819, 1029, 1107, 1174, 1350, 2112 |
| GsuI | CTGGAG | 2 cut(s) | 762, 1461 |
| HaeII | RGCGCY | 1 cut(s) | 782 |
| HaeIII | GGCC | 9 cut(s) | 61, 178, 915, 1506, 1688, 1911, 1929, 2410, 3031 |
| HapII | CCGG | 7 cut(s) | 1256, 1271, 1286, 1524, 2192, 2411, 2912 |
| HhaI | GCGC | 1 cut(s) | 781 |
| Hin1I | GRCGYC | 1 cut(s) | 779 |
| Hin6I | GCGC | 1 cut(s) | 779 |
| HinP1I | GCGC | 1 cut(s) | 779 |
| HincII | GTYRAC | 3 cut(s) | 1534, 1965, 2767 |
| HindII | GTYRAC | 3 cut(s) | 1534, 1965, 2767 |
| HindIII | AAGCTT | 1 cut(s) | 970 |
| HinfI | GANTC | 8 cut(s) | 147, 315, 392, 902, 1012, 1159, 2584, 2645 |
| HpaII | CCGG | 7 cut(s) | 1256, 1271, 1286, 1524, 2192, 2411, 2912 |
| HphI | GGTGA | 6 cut(s) | 650, 1544, 1969, 2011, 2206, 2433 |
| Hpy188I | TCNGA | 8 cut(s) | 266, 433, 712, 907, 1140, 2133, 2460, 2650 |
| HpyCH4III | ACNGT | 7 cut(s) | 169, 614, 673, 1716, 2377, 2800, 2902 |
| HpyCH4IV | ACGT | 5 cut(s) | 1218, 1354, 1564, 2305, 3123 |
| HpyF3I | CTNAG | 6 cut(s) | 78, 1454, 1623, 2457, 2607, 2878 |
| HpySE526I | ACGT | 5 cut(s) | 1218, 1354, 1564, 2305, 3123 |
| Hsp92I | GRCGYC | 1 cut(s) | 779 |
| HspAI | GCGC | 1 cut(s) | 779 |
| KasI | GGCGCC | 1 cut(s) | 778 |
| KpnI | GGTACC | 2 cut(s) | 2486, 2716 |
| KroI | GCCGGC | 3 cut(s) | 1255, 1270, 1523 |
| KroNI | GCCGGC | 3 cut(s) | 1257, 1272, 1525 |
| Kzo9I | GATC | 8 cut(s) | 325, 543, 568, 1638, 1822, 2679, 2881, 2938 |
| LmnI | GCTCC | 4 cut(s) | 600, 1058, 1495, 2449 |
| Lsp1109I | GCAGC | 4 cut(s) | 805, 1093, 1336, 2123 |
| LweI | GCATC | 5 cut(s) | 669, 830, 875, 2134, 2503 |
| MaeI | CTAG | 7 cut(s) | 57, 1449, 1560, 1764, 1769, 1803, 2829 |
| MaeII | ACGT | 5 cut(s) | 1218, 1354, 1564, 2305, 3123 |
| MaeIII | GTNAC | 7 cut(s) | 161, 637, 1357, 1710, 1888, 2173, 2955 |
| MalI | GATC | 8 cut(s) | 327, 545, 570, 1640, 1824, 2681, 2883, 2940 |
| MboI | GATC | 8 cut(s) | 325, 543, 568, 1638, 1822, 2679, 2881, 2938 |
| MflI | RGATCY | 2 cut(s) | 543, 2938 |
| MhlI | GDGCHC | 3 cut(s) | 193, 285, 2571 |
| MlsI | TGGCCA | 1 cut(s) | 1929 |
| MluNI | TGGCCA | 1 cut(s) | 1929 |
| Mly113I | GGCGCC | 1 cut(s) | 779 |
| MlyI | GAGTC | 3 cut(s) | 1006, 1153, 2593 |
| MmeI | TCCRAC | 3 cut(s) | 2378, 2761, 3072 |
| Mox20I | TGGCCA | 1 cut(s) | 1929 |
| Mph1103I | ATGCAT | 3 cut(s) | 1681, 1845, 2552 |
| MroNI | GCCGGC | 3 cut(s) | 1255, 1270, 1523 |
| MroXI | GAANNNNTTC | 1 cut(s) | 545 |
| MscI | TGGCCA | 1 cut(s) | 1929 |
| MseI | TTAA | 9 cut(s) | 507, 695, 974, 1665, 1728, 1970, 2160, 2520, 3084 |
| MslI | CAYNNNNRTG | 7 cut(s) | 237, 507, 518, 671, 2243, 2741, 2823 |
| Msp20I | TGGCCA | 1 cut(s) | 1929 |
| MspA1I | CMGCKG | 3 cut(s) | 836, 1106, 1820 |
| MspI | CCGG | 7 cut(s) | 1256, 1271, 1286, 1524, 2192, 2411, 2912 |
| MspR9I | CCNGG | 3 cut(s) | 1607, 1953, 2850 |
| MvaI | CCWGG | 3 cut(s) | 1607, 1953, 2850 |
| NaeI | GCCGGC | 3 cut(s) | 1257, 1272, 1525 |
| NarI | GGCGCC | 1 cut(s) | 779 |
| NcoI | CCATGG | 1 cut(s) | 2388 |
| NdeI | CATATG | 1 cut(s) | 1018 |
| NdeII | GATC | 8 cut(s) | 325, 543, 568, 1638, 1822, 2679, 2881, 2938 |
| NgoMIV | GCCGGC | 3 cut(s) | 1255, 1270, 1523 |
| NheI | GCTAGC | 1 cut(s) | 1802 |
| NlaIV | GGNNCC | 8 cut(s) | 596, 780, 2294, 2484, 2714, 2868, 2910, 2940 |
| NmeAIII | GCCGAG | 7 cut(s) | 465, 1276, 1291, 1306, 1532, 2140, 2948 |
| NmuCI | GTSAC | 3 cut(s) | 161, 1710, 1888 |
| NsiI | ATGCAT | 3 cut(s) | 1681, 1845, 2552 |
| NspI | RCATGY | 3 cut(s) | 736, 1333, 2554 |
| NspV | TTCGAA | 1 cut(s) | 46 |
| OliI | CACNNNNGTG | 3 cut(s) | 237, 518, 2823 |
| PagI | TCATGA | 1 cut(s) | 892 |
| PceI | AGGCCT | 2 cut(s) | 61, 178 |
| PciI | ACATGT | 1 cut(s) | 1329 |
| PdiI | GCCGGC | 3 cut(s) | 1257, 1272, 1525 |
| PdmI | GAANNNNTTC | 1 cut(s) | 545 |
| PfeI | GAWTC | 5 cut(s) | 147, 315, 392, 902, 2645 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1730 |
| PkrI | GCNGC | 6 cut(s) | 820, 1030, 1108, 1175, 1351, 2113 |
| PleI | GAGTC | 3 cut(s) | 1006, 1153, 2592 |
| PluTI | GGCGCC | 1 cut(s) | 782 |
| PmaCI | CACGTG | 1 cut(s) | 1355 |
| PmlI | CACGTG | 1 cut(s) | 1355 |
| PpsI | GAGTC | 3 cut(s) | 1006, 1153, 2592 |
| Ppu21I | YACGTR | 3 cut(s) | 1355, 2306, 3124 |
| PscI | ACATGT | 1 cut(s) | 1329 |
| PsiI | TTATAA | 1 cut(s) | 1598 |
| Psp124BI | GAGCTC | 1 cut(s) | 2571 |
| Psp6I | CCWGG | 3 cut(s) | 1605, 1951, 2848 |
| PspCI | CACGTG | 1 cut(s) | 1355 |
| PspEI | GGTNACC | 1 cut(s) | 2955 |
| PspGI | CCWGG | 3 cut(s) | 1605, 1951, 2848 |
| PspN4I | GGNNCC | 8 cut(s) | 596, 780, 2294, 2484, 2714, 2868, 2910, 2940 |
| PspPI | GGNCC | 3 cut(s) | 913, 1686, 2293 |
| PstNI | CAGNNNCTG | 1 cut(s) | 2099 |
| PsuI | RGATCY | 2 cut(s) | 543, 2938 |
| PvuII | CAGCTG | 2 cut(s) | 836, 1106 |
| RseI | CAYNNNNRTG | 7 cut(s) | 237, 507, 518, 671, 2243, 2741, 2823 |
| SacI | GAGCTC | 1 cut(s) | 2571 |
| SaqAI | TTAA | 9 cut(s) | 507, 695, 974, 1665, 1728, 1970, 2160, 2520, 3084 |
| SatI | GCNGC | 6 cut(s) | 819, 1029, 1107, 1174, 1350, 2112 |
| Sau3AI | GATC | 8 cut(s) | 325, 543, 568, 1638, 1822, 2679, 2881, 2938 |
| Sau96I | GGNCC | 3 cut(s) | 913, 1686, 2293 |
| ScaI | AGTACT | 1 cut(s) | 1617 |
| SchI | GAGTC | 3 cut(s) | 1006, 1153, 2593 |
| ScrFI | CCNGG | 3 cut(s) | 1607, 1953, 2850 |
| SduI | GDGCHC | 3 cut(s) | 193, 285, 2571 |
| SfaNI | GCATC | 5 cut(s) | 669, 830, 875, 2134, 2503 |
| SfcI | CTRYAG | 3 cut(s) | 610, 2232, 2961 |
| SfoI | GGCGCC | 1 cut(s) | 780 |
| SfuI | TTCGAA | 1 cut(s) | 46 |
| SinI | GGWCC | 1 cut(s) | 2293 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 237, 507, 518, 671, 2243, 2741, 2823 |
| SmlI | CTYRAG | 1 cut(s) | 2588 |
| SmoI | CTYRAG | 1 cut(s) | 2588 |
| SnaBI | TACGTA | 1 cut(s) | 3124 |
| SseBI | AGGCCT | 2 cut(s) | 61, 178 |
| SsiI | CCGC | 9 cut(s) | 101, 319, 699, 1028, 1174, 1207, 1818, 2041, 2953 |
| SspDI | GGCGCC | 1 cut(s) | 778 |
| SspI | AATATT | 1 cut(s) | 3077 |
| SspMI | CTAG | 7 cut(s) | 57, 1449, 1560, 1764, 1769, 1803, 2829 |
| SstI | GAGCTC | 1 cut(s) | 2571 |
| StuI | AGGCCT | 2 cut(s) | 61, 178 |
| StyD4I | CCNGG | 3 cut(s) | 1605, 1951, 2848 |
| StyI | CCWWGG | 3 cut(s) | 800, 2388, 3061 |
| TaaI | ACNGT | 7 cut(s) | 169, 614, 673, 1716, 2377, 2800, 2902 |
| TaiI | ACGT | 5 cut(s) | 1221, 1357, 1567, 2308, 3126 |
| TaqI | TCGA | 8 cut(s) | 12, 46, 52, 762, 869, 1917, 2312, 2490 |
| TatI | WGTACW | 2 cut(s) | 1615, 2125 |
| TauI | GCSGC | 2 cut(s) | 1031, 1176 |
| TfiI | GAWTC | 5 cut(s) | 147, 315, 392, 902, 2645 |
| Tru1I | TTAA | 9 cut(s) | 507, 695, 974, 1665, 1728, 1970, 2160, 2520, 3084 |
| Tru9I | TTAA | 9 cut(s) | 507, 695, 974, 1665, 1728, 1970, 2160, 2520, 3084 |
| TscAI | CASTG | 7 cut(s) | 172, 619, 678, 868, 1897, 2146, 2689 |
| TseFI | GTSAC | 3 cut(s) | 161, 1710, 1888 |
| TseI | GCWGC | 4 cut(s) | 818, 1106, 1349, 2111 |
| Tsp45I | GTSAC | 3 cut(s) | 161, 1710, 1888 |
| TspGWI | ACGGA | 2 cut(s) | 356, 1322 |
| TspRI | CASTG | 7 cut(s) | 172, 619, 678, 868, 1897, 2146, 2689 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1730 |
| VpaK11BI | GGWCC | 1 cut(s) | 2293 |
| XapI | RAATTY | 5 cut(s) | 107, 260, 1124, 1373, 3156 |
| XceI | RCATGY | 3 cut(s) | 736, 1333, 2554 |
| XmiI | GTMKAC | 1 cut(s) | 794 |
| XmnI | GAANNNNTTC | 1 cut(s) | 545 |
| XspI | CTAG | 7 cut(s) | 57, 1449, 1560, 1764, 1769, 1803, 2829 |
| ZrmI | AGTACT | 1 cut(s) | 1617 |
| Zsp2I | ATGCAT | 3 cut(s) | 1681, 1845, 2552 |