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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2132 |
| Acc16I | TGCGCA | 1 cut(s) | 1928 |
| Acc36I | ACCTGC | 1 cut(s) | 2257 |
| Acc65I | GGTACC | 1 cut(s) | 1862 |
| AccB1I | GGYRCC | 1 cut(s) | 1862 |
| AccIII | TCCGGA | 1 cut(s) | 94 |
| AciI | CCGC | 7 cut(s) | 160, 353, 1399, 1934, 2000, 2081, 2260 |
| AclWI | GGATC | 5 cut(s) | 86, 975, 1151, 1423, 2028 |
| AcoI | YGGCCR | 3 cut(s) | 340, 412, 589 |
| AcsI | RAATTY | 1 cut(s) | 453 |
| AcuI | CTGAAG | 3 cut(s) | 132, 456, 1239 |
| AfaI | GTAC | 5 cut(s) | 404, 915, 1725, 1864, 2046 |
| AfeI | AGCGCT | 1 cut(s) | 1544 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 101, 166, 584, 662, 2024, 2242, 2266 |
| AflII | CTTAAG | 1 cut(s) | 11 |
| AflIII | ACRYGT | 1 cut(s) | 505 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 506 |
| AjiI | CACGTC | 1 cut(s) | 508 |
| AjnI | CCWGG | 5 cut(s) | 591, 1604, 1713, 1744, 1865 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 206, 238 |
| AluBI | AGCT | 9 cut(s) | 10, 122, 539, 546, 1283, 1738, 1895, 1986, 2219 |
| AluI | AGCT | 9 cut(s) | 10, 122, 539, 546, 1283, 1738, 1895, 1986, 2219 |
| Alw21I | GWGCWC | 1 cut(s) | 1700 |
| Alw26I | GTCTC | 2 cut(s) | 2075, 2091 |
| Alw44I | GTGCAC | 1 cut(s) | 1696 |
| AlwI | GGATC | 5 cut(s) | 86, 975, 1151, 1423, 2028 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1895 |
| Aor13HI | TCCGGA | 1 cut(s) | 94 |
| Aor51HI | AGCGCT | 1 cut(s) | 1544 |
| AoxI | GGCC | 8 cut(s) | 135, 340, 347, 364, 412, 589, 835, 1019 |
| ApaLI | GTGCAC | 1 cut(s) | 1696 |
| ApeKI | GCWGC | 6 cut(s) | 1622, 1889, 1892, 1919, 2120, 2216 |
| ApoI | RAATTY | 1 cut(s) | 453 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 661, 693 |
| AseI | ATTAAT | 1 cut(s) | 2136 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 221 |
| Asp718I | GGTACC | 1 cut(s) | 1862 |
| AspLEI | GCGC | 2 cut(s) | 1545, 1929 |
| AspS9I | GGNCC | 8 cut(s) | 136, 365, 575, 836, 1020, 1195, 1306, 1316 |
| AsuC2I | CCSGG | 1 cut(s) | 1199 |
| AsuHPI | GGTGA | 5 cut(s) | 194, 1391, 1535, 1771, 2168 |
| AvaII | GGWCC | 4 cut(s) | 575, 1195, 1306, 1316 |
| AxyI | CCTNAGG | 1 cut(s) | 209 |
| BaeGI | GKGCMC | 1 cut(s) | 1700 |
| BalI | TGGCCA | 3 cut(s) | 342, 414, 591 |
| BanI | GGYRCC | 1 cut(s) | 1862 |
| BanII | GRGCYC | 1 cut(s) | 2248 |
| BbsI | GAAGAC | 3 cut(s) | 134, 492, 2179 |
| Bbv12I | GWGCWC | 1 cut(s) | 1700 |
| BbvCI | CCTCAGC | 1 cut(s) | 123 |
| BbvI | GCAGC | 6 cut(s) | 1634, 1901, 1904, 1931, 2132, 2228 |
| BciT130I | CCWGG | 5 cut(s) | 593, 1606, 1715, 1746, 1867 |
| BciVI | GTATCC | 2 cut(s) | 954, 1255 |
| BclI | TGATCA | 2 cut(s) | 631, 1035 |
| BcnI | CCSGG | 1 cut(s) | 1199 |
| BcoDI | GTCTC | 2 cut(s) | 2075, 2091 |
| BfaI | CTAG | 2 cut(s) | 1329, 2284 |
| BfmI | CTRYAG | 2 cut(s) | 1467, 1577 |
| BfoI | RGCGCY | 1 cut(s) | 1546 |
| BfrI | CTTAAG | 1 cut(s) | 11 |
| BfuAI | ACCTGC | 1 cut(s) | 2257 |
| BfuI | GTATCC | 2 cut(s) | 954, 1255 |
| BisI | GCNGC | 6 cut(s) | 1623, 1890, 1893, 1920, 2121, 2217 |
| BlsI | GCNGC | 6 cut(s) | 1624, 1891, 1894, 1921, 2122, 2218 |
| BmcAI | AGTACT | 1 cut(s) | 1725 |
| Bme1390I | CCNGG | 6 cut(s) | 593, 1199, 1606, 1715, 1746, 1867 |
| Bme18I | GGWCC | 4 cut(s) | 575, 1195, 1306, 1316 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 506 |
| BmgBI | CACGTC | 1 cut(s) | 508 |
| BmgT120I | GGNCC | 8 cut(s) | 136, 365, 575, 836, 1020, 1195, 1306, 1316 |
| BmiI | GGNNCC | 6 cut(s) | 1196, 1197, 1536, 1611, 1864, 2271 |
| BmrFI | CCNGG | 6 cut(s) | 593, 1199, 1606, 1715, 1746, 1867 |
| BmrI | ACTGGG | 2 cut(s) | 171, 1849 |
| BmsI | GCATC | 6 cut(s) | 280, 451, 769, 1192, 1224, 1494 |
| BmuI | ACTGGG | 2 cut(s) | 171, 1849 |
| BpiI | GAAGAC | 3 cut(s) | 134, 492, 2179 |
| BpmI | CTGGAG | 2 cut(s) | 225, 2202 |
| Bpu10I | CCTNAGC | 2 cut(s) | 123, 1539 |
| BpuMI | CCSGG | 1 cut(s) | 1199 |
| Bsa29I | ATCGAT | 1 cut(s) | 2032 |
| BsaBI | GATNNNNATC | 1 cut(s) | 1437 |
| BsaI | GGTCTC | 2 cut(s) | 2075, 2091 |
| BsaJI | CCNNGG | 5 cut(s) | 36, 387, 956, 1605, 1744 |
| BsaWI | WCCGGW | 1 cut(s) | 94 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 206, 223, 236, 253, 954, 984 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 101, 166, 584, 662, 2024, 2242, 2266 |
| Bse118I | RCCGGY | 1 cut(s) | 362 |
| Bse1I | ACTGG | 8 cut(s) | 177, 242, 415, 803, 1270, 1844, 2151, 2185 |
| Bse21I | CCTNAGG | 1 cut(s) | 209 |
| Bse3DI | GCAATG | 1 cut(s) | 2273 |
| Bse8I | GATNNNNATC | 1 cut(s) | 1437 |
| BseAI | TCCGGA | 1 cut(s) | 94 |
| BseBI | CCWGG | 5 cut(s) | 593, 1606, 1715, 1746, 1867 |
| BseCI | ATCGAT | 1 cut(s) | 2032 |
| BseDI | CCNNGG | 5 cut(s) | 36, 387, 956, 1605, 1744 |
| BseGI | GGATG | 5 cut(s) | 760, 795, 952, 1451, 1825 |
| BseJI | GATNNNNATC | 1 cut(s) | 1437 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 101, 166, 584, 662, 2024, 2242, 2266 |
| BseMI | GCAATG | 1 cut(s) | 2273 |
| BseMII | CTCAG | 6 cut(s) | 114, 287, 333, 556, 1370, 1530 |
| BseNI | ACTGG | 8 cut(s) | 177, 242, 415, 803, 1270, 1844, 2151, 2185 |
| BseRI | GAGGAG | 1 cut(s) | 1937 |
| BseSI | GKGCMC | 1 cut(s) | 1700 |
| BseXI | GCAGC | 6 cut(s) | 1634, 1901, 1904, 1931, 2132, 2228 |
| BseYI | CCCAGC | 1 cut(s) | 2166 |
| BsgI | GTGCAG | 2 cut(s) | 1938, 2139 |
| BshFI | GGCC | 8 cut(s) | 137, 342, 349, 366, 414, 591, 837, 1021 |
| BshNI | GGYRCC | 1 cut(s) | 1862 |
| BshVI | ATCGAT | 1 cut(s) | 2032 |
| BsiHKAI | GWGCWC | 1 cut(s) | 1700 |
| BsiSI | CCGG | 3 cut(s) | 95, 363, 1199 |
| BslFI | GGGAC | 4 cut(s) | 513, 1181, 1181, 2097 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 101, 166, 584, 662, 2024, 2242, 2266 |
| BsmAI | GTCTC | 2 cut(s) | 2075, 2091 |
| BsmFI | GGGAC | 4 cut(s) | 513, 1181, 1181, 2097 |
| BsnI | GGCC | 8 cut(s) | 137, 342, 349, 366, 414, 591, 837, 1021 |
| Bso31I | GGTCTC | 2 cut(s) | 2075, 2091 |
| Bsp1286I | GDGCHC | 2 cut(s) | 1700, 2248 |
| Bsp13I | TCCGGA | 1 cut(s) | 94 |
| Bsp1407I | TGTACA | 1 cut(s) | 402 |
| Bsp143I | GATC | 9 cut(s) | 91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033 |
| BspACI | CCGC | 7 cut(s) | 160, 353, 1399, 1934, 2000, 2081, 2260 |
| BspANI | GGCC | 8 cut(s) | 137, 342, 349, 366, 414, 591, 837, 1021 |
| BspCNI | CTCAG | 6 cut(s) | 115, 288, 332, 555, 1369, 1531 |
| BspDI | ATCGAT | 1 cut(s) | 2032 |
| BspEI | TCCGGA | 1 cut(s) | 94 |
| BspHI | TCATGA | 1 cut(s) | 2190 |
| BspLI | GGNNCC | 6 cut(s) | 1196, 1197, 1536, 1611, 1864, 2271 |
| BspMAI | CTGCAG | 1 cut(s) | 1471 |
| BspMI | ACCTGC | 1 cut(s) | 2257 |
| BspPI | GGATC | 5 cut(s) | 86, 975, 1151, 1423, 2028 |
| BspQI | GCTCTTC | 3 cut(s) | 108, 432, 1056 |
| BspT107I | GGYRCC | 1 cut(s) | 1862 |
| BspTI | CTTAAG | 1 cut(s) | 11 |
| BspTNI | GGTCTC | 2 cut(s) | 2075, 2091 |
| BsrDI | GCAATG | 1 cut(s) | 2273 |
| BsrFI | RCCGGY | 1 cut(s) | 362 |
| BsrGI | TGTACA | 1 cut(s) | 402 |
| BsrI | ACTGG | 8 cut(s) | 177, 242, 415, 803, 1270, 1844, 2151, 2185 |
| BssAI | RCCGGY | 1 cut(s) | 362 |
| BssECI | CCNNGG | 5 cut(s) | 36, 387, 956, 1605, 1744 |
| BssMI | GATC | 9 cut(s) | 91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033 |
| BssT1I | CCWWGG | 2 cut(s) | 387, 956 |
| Bst2UI | CCWGG | 5 cut(s) | 593, 1606, 1715, 1746, 1867 |
| Bst4CI | ACNGT | 4 cut(s) | 491, 1094, 1641, 1728 |
| Bst6I | CTCTTC | 5 cut(s) | 108, 126, 432, 1056, 2115 |
| BstAFI | CTTAAG | 1 cut(s) | 11 |
| BstAUI | TGTACA | 1 cut(s) | 402 |
| BstDEI | CTNAG | 7 cut(s) | 123, 209, 296, 319, 542, 1356, 1539 |
| BstEII | GGTNACC | 1 cut(s) | 182 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 2022 |
| BstF5I | GGATG | 5 cut(s) | 760, 795, 952, 1451, 1825 |
| BstH2I | RGCGCY | 1 cut(s) | 1546 |
| BstHHI | GCGC | 2 cut(s) | 1545, 1929 |
| BstKTI | GATC | 9 cut(s) | 94, 634, 970, 1038, 1159, 1418, 1435, 1441, 2036 |
| BstMAI | GTCTC | 2 cut(s) | 2075, 2091 |
| BstMBI | GATC | 9 cut(s) | 91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 329, 359, 1280, 1289, 1398, 1622, 1715, 2087 |
| BstNI | CCWGG | 5 cut(s) | 593, 1606, 1715, 1746, 1867 |
| BstNSI | RCATGY | 3 cut(s) | 802, 1396, 2206 |
| BstPI | GGTNACC | 1 cut(s) | 182 |
| BstSCI | CCNGG | 6 cut(s) | 591, 1197, 1604, 1713, 1744, 1865 |
| BstSFI | CTRYAG | 2 cut(s) | 1467, 1577 |
| BstSLI | GKGCMC | 1 cut(s) | 1700 |
| BstV1I | GCAGC | 6 cut(s) | 1634, 1901, 1904, 1931, 2132, 2228 |
| BstV2I | GAAGAC | 3 cut(s) | 134, 492, 2179 |
| BstX2I | RGATCY | 2 cut(s) | 967, 1156 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 732 |
| BstYI | RGATCY | 2 cut(s) | 967, 1156 |
| Bsu15I | ATCGAT | 1 cut(s) | 2032 |
| Bsu36I | CCTNAGG | 1 cut(s) | 209 |
| BsuI | GTATCC | 2 cut(s) | 954, 1255 |
| BsuRI | GGCC | 8 cut(s) | 137, 342, 349, 366, 414, 591, 837, 1021 |
| BsuTUI | ATCGAT | 1 cut(s) | 2032 |
| BtgZI | GCGATG | 2 cut(s) | 351, 428 |
| BtrI | CACGTC | 1 cut(s) | 508 |
| BtsCI | GGATG | 5 cut(s) | 760, 795, 952, 1451, 1825 |
| BtsI | GCAGTG | 1 cut(s) | 1464 |
| BtsIMutI | CAGTG | 5 cut(s) | 184, 293, 810, 1464, 2178 |
| BveI | ACCTGC | 1 cut(s) | 2257 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1895 |
| CciI | TCATGA | 1 cut(s) | 2190 |
| CfoI | GCGC | 2 cut(s) | 1545, 1929 |
| Cfr10I | RCCGGY | 1 cut(s) | 362 |
| Cfr13I | GGNCC | 8 cut(s) | 136, 365, 575, 836, 1020, 1195, 1306, 1316 |
| ClaI | ATCGAT | 1 cut(s) | 2032 |
| CsiI | ACCWGGT | 1 cut(s) | 1865 |
| Csp6I | GTAC | 5 cut(s) | 403, 914, 1724, 1863, 2045 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 160, 195, 1573, 1608 |
| CviQI | GTAC | 5 cut(s) | 403, 914, 1724, 1863, 2045 |
| DdeI | CTNAG | 7 cut(s) | 123, 209, 296, 319, 542, 1356, 1539 |
| DpnI | GATC | 9 cut(s) | 93, 633, 969, 1037, 1158, 1417, 1434, 1440, 2035 |
| DpnII | GATC | 9 cut(s) | 91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033 |
| DraI | TTTAAA | 1 cut(s) | 1479 |
| DriI | GACNNNNNGTC | 1 cut(s) | 506 |
| EaeI | YGGCCR | 3 cut(s) | 340, 412, 589 |
| Eam1104I | CTCTTC | 5 cut(s) | 108, 126, 432, 1056, 2115 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 506 |
| EarI | CTCTTC | 5 cut(s) | 108, 126, 432, 1056, 2115 |
| Eco130I | CCWWGG | 2 cut(s) | 387, 956 |
| Eco24I | GRGCYC | 1 cut(s) | 2248 |
| Eco31I | GGTCTC | 2 cut(s) | 2075, 2091 |
| Eco47I | GGWCC | 4 cut(s) | 575, 1195, 1306, 1316 |
| Eco47III | AGCGCT | 1 cut(s) | 1544 |
| Eco57I | CTGAAG | 3 cut(s) | 132, 456, 1239 |
| Eco81I | CCTNAGG | 1 cut(s) | 209 |
| Eco91I | GGTNACC | 1 cut(s) | 182 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 2022 |
| EcoO109I | RGGNCCY | 2 cut(s) | 575, 1195 |
| EcoO65I | GGTNACC | 1 cut(s) | 182 |
| EcoRII | CCWGG | 5 cut(s) | 591, 1604, 1713, 1744, 1865 |
| EcoT14I | CCWWGG | 2 cut(s) | 387, 956 |
| EcoT22I | ATGCAT | 1 cut(s) | 762 |
| EcoT38I | GRGCYC | 1 cut(s) | 2248 |
| ErhI | CCWWGG | 2 cut(s) | 387, 956 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1272, 1304 |
| FaqI | GGGAC | 4 cut(s) | 513, 1181, 1181, 2097 |
| FauI | CCCGC | 1 cut(s) | 2074 |
| FauNDI | CATATG | 2 cut(s) | 395, 1858 |
| FbaI | TGATCA | 2 cut(s) | 631, 1035 |
| Fnu4HI | GCNGC | 6 cut(s) | 1623, 1890, 1893, 1920, 2121, 2217 |
| FokI | GGATG | 5 cut(s) | 747, 802, 939, 1458, 1832 |
| FriOI | GRGCYC | 1 cut(s) | 2248 |
| Fsp4HI | GCNGC | 6 cut(s) | 1623, 1890, 1893, 1920, 2121, 2217 |
| FspBI | CTAG | 2 cut(s) | 1329, 2284 |
| FspI | TGCGCA | 1 cut(s) | 1928 |
| GlaI | GCGC | 2 cut(s) | 1544, 1928 |
| GluI | GCNGC | 6 cut(s) | 1623, 1890, 1893, 1920, 2121, 2217 |
| GsaI | CCCAGC | 1 cut(s) | 2170 |
| GsuI | CTGGAG | 2 cut(s) | 225, 2202 |
| HaeII | RGCGCY | 1 cut(s) | 1546 |
| HaeIII | GGCC | 8 cut(s) | 137, 342, 349, 366, 414, 591, 837, 1021 |
| HapII | CCGG | 3 cut(s) | 95, 363, 1199 |
| HhaI | GCGC | 2 cut(s) | 1545, 1929 |
| Hin6I | GCGC | 2 cut(s) | 1543, 1927 |
| HinP1I | GCGC | 2 cut(s) | 1543, 1927 |
| HindIII | AAGCTT | 2 cut(s) | 8, 1281 |
| HinfI | GANTC | 6 cut(s) | 32, 98, 625, 736, 1553, 2184 |
| HpaII | CCGG | 3 cut(s) | 95, 363, 1199 |
| HphI | GGTGA | 5 cut(s) | 194, 1391, 1535, 1771, 2168 |
| Hpy166II | GTNNAC | 4 cut(s) | 310, 796, 1422, 1698 |
| Hpy188I | TCNGA | 8 cut(s) | 71, 480, 569, 1040, 1432, 1459, 1513, 1650 |
| Hpy8I | GTNNAC | 4 cut(s) | 310, 796, 1422, 1698 |
| Hpy99I | CGWCG | 1 cut(s) | 512 |
| HpyCH4III | ACNGT | 4 cut(s) | 491, 1094, 1641, 1728 |
| HpyCH4IV | ACGT | 1 cut(s) | 507 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 329, 359, 1280, 1289, 1398, 1622, 1715, 2087 |
| HpyF3I | CTNAG | 7 cut(s) | 123, 209, 296, 319, 542, 1356, 1539 |
| HpySE526I | ACGT | 1 cut(s) | 507 |
| HspAI | GCGC | 2 cut(s) | 1543, 1927 |
| KflI | GGGWCCC | 1 cut(s) | 1195 |
| Kpn2I | TCCGGA | 1 cut(s) | 94 |
| KpnI | GGTACC | 1 cut(s) | 1866 |
| KroI | GCCGGC | 1 cut(s) | 362 |
| KroNI | GCCGGC | 1 cut(s) | 364 |
| Ksp22I | TGATCA | 2 cut(s) | 631, 1035 |
| Kzo9I | GATC | 9 cut(s) | 91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033 |
| LguI | GCTCTTC | 3 cut(s) | 108, 432, 1056 |
| Lsp1109I | GCAGC | 6 cut(s) | 1634, 1901, 1904, 1931, 2132, 2228 |
| LweI | GCATC | 6 cut(s) | 280, 451, 769, 1192, 1224, 1494 |
| MabI | ACCWGGT | 1 cut(s) | 1865 |
| MaeI | CTAG | 2 cut(s) | 1329, 2284 |
| MaeII | ACGT | 1 cut(s) | 507 |
| MaeIII | GTNAC | 6 cut(s) | 182, 461, 515, 643, 1057, 1379 |
| MalI | GATC | 9 cut(s) | 93, 633, 969, 1037, 1158, 1417, 1434, 1440, 2035 |
| MboI | GATC | 9 cut(s) | 91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033 |
| MfeI | CAATTG | 2 cut(s) | 860, 901 |
| MflI | RGATCY | 2 cut(s) | 967, 1156 |
| MhlI | GDGCHC | 2 cut(s) | 1700, 2248 |
| MlsI | TGGCCA | 3 cut(s) | 342, 414, 591 |
| MluNI | TGGCCA | 3 cut(s) | 342, 414, 591 |
| MlyI | GAGTC | 1 cut(s) | 2193 |
| MmeI | TCCRAC | 3 cut(s) | 49, 1127, 1673 |
| Mox20I | TGGCCA | 3 cut(s) | 342, 414, 591 |
| Mph1103I | ATGCAT | 1 cut(s) | 762 |
| MroI | TCCGGA | 1 cut(s) | 94 |
| MroNI | GCCGGC | 1 cut(s) | 362 |
| MroXI | GAANNNNTTC | 1 cut(s) | 221 |
| MscI | TGGCCA | 3 cut(s) | 342, 414, 591 |
| MseI | TTAA | 6 cut(s) | 12, 420, 1478, 1643, 1731, 2136 |
| MslI | CAYNNNNRTG | 7 cut(s) | 398, 480, 554, 639, 803, 924, 1686 |
| Msp20I | TGGCCA | 3 cut(s) | 342, 414, 591 |
| MspA1I | CMGCKG | 1 cut(s) | 1895 |
| MspCI | CTTAAG | 1 cut(s) | 11 |
| MspI | CCGG | 3 cut(s) | 95, 363, 1199 |
| MspR9I | CCNGG | 6 cut(s) | 593, 1199, 1606, 1715, 1746, 1867 |
| MunI | CAATTG | 2 cut(s) | 860, 901 |
| MvaI | CCWGG | 5 cut(s) | 593, 1606, 1715, 1746, 1867 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 329, 359, 1280, 1289, 1398, 1622, 1715, 2087 |
| NaeI | GCCGGC | 1 cut(s) | 364 |
| NciI | CCSGG | 1 cut(s) | 1199 |
| NdeI | CATATG | 2 cut(s) | 395, 1858 |
| NdeII | GATC | 9 cut(s) | 91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033 |
| NgoMIV | GCCGGC | 1 cut(s) | 362 |
| NlaIV | GGNNCC | 6 cut(s) | 1196, 1197, 1536, 1611, 1864, 2271 |
| NmeAIII | GCCGAG | 1 cut(s) | 17 |
| NmuCI | GTSAC | 4 cut(s) | 182, 515, 643, 1379 |
| NsbI | TGCGCA | 1 cut(s) | 1928 |
| NsiI | ATGCAT | 1 cut(s) | 762 |
| NspI | RCATGY | 3 cut(s) | 802, 1396, 2206 |
| PaeI | GCATGC | 2 cut(s) | 1396, 2206 |
| PagI | TCATGA | 1 cut(s) | 2190 |
| PciSI | GCTCTTC | 3 cut(s) | 108, 432, 1056 |
| PdiI | GCCGGC | 1 cut(s) | 364 |
| PdmI | GAANNNNTTC | 1 cut(s) | 221 |
| PfeI | GAWTC | 5 cut(s) | 32, 98, 625, 736, 1553 |
| PfoI | TCCNGGA | 1 cut(s) | 1197 |
| PkrI | GCNGC | 6 cut(s) | 1624, 1891, 1894, 1921, 2122, 2218 |
| PleI | GAGTC | 1 cut(s) | 2192 |
| PpsI | GAGTC | 1 cut(s) | 2192 |
| PpuMI | RGGWCCY | 2 cut(s) | 575, 1195 |
| PshBI | ATTAAT | 1 cut(s) | 2136 |
| PsiI | TTATAA | 1 cut(s) | 2132 |
| Psp5II | RGGWCCY | 2 cut(s) | 575, 1195 |
| Psp6I | CCWGG | 5 cut(s) | 591, 1604, 1713, 1744, 1865 |
| PspEI | GGTNACC | 1 cut(s) | 182 |
| PspFI | CCCAGC | 1 cut(s) | 2166 |
| PspGI | CCWGG | 5 cut(s) | 591, 1604, 1713, 1744, 1865 |
| PspN4I | GGNNCC | 6 cut(s) | 1196, 1197, 1536, 1611, 1864, 2271 |
| PspPI | GGNCC | 8 cut(s) | 136, 365, 575, 836, 1020, 1195, 1306, 1316 |
| PspPPI | RGGWCCY | 2 cut(s) | 575, 1195 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1082, 1114 |
| PstI | CTGCAG | 1 cut(s) | 1471 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1895 |
| PsuI | RGATCY | 2 cut(s) | 967, 1156 |
| PvuII | CAGCTG | 1 cut(s) | 1895 |
| RsaI | GTAC | 5 cut(s) | 404, 915, 1725, 1864, 2046 |
| RsaNI | GTAC | 5 cut(s) | 403, 914, 1724, 1863, 2045 |
| RseI | CAYNNNNRTG | 7 cut(s) | 398, 480, 554, 639, 803, 924, 1686 |
| SapI | GCTCTTC | 3 cut(s) | 108, 432, 1056 |
| SaqAI | TTAA | 6 cut(s) | 12, 420, 1478, 1643, 1731, 2136 |
| SatI | GCNGC | 6 cut(s) | 1623, 1890, 1893, 1920, 2121, 2217 |
| Sau3AI | GATC | 9 cut(s) | 91, 631, 967, 1035, 1156, 1415, 1432, 1438, 2033 |
| Sau96I | GGNCC | 8 cut(s) | 136, 365, 575, 836, 1020, 1195, 1306, 1316 |
| ScaI | AGTACT | 1 cut(s) | 1725 |
| SchI | GAGTC | 1 cut(s) | 2193 |
| ScrFI | CCNGG | 6 cut(s) | 593, 1199, 1606, 1715, 1746, 1867 |
| SduI | GDGCHC | 2 cut(s) | 1700, 2248 |
| SexAI | ACCWGGT | 1 cut(s) | 1865 |
| SfaNI | GCATC | 6 cut(s) | 280, 451, 769, 1192, 1224, 1494 |
| SfcI | CTRYAG | 2 cut(s) | 1467, 1577 |
| SinI | GGWCC | 4 cut(s) | 575, 1195, 1306, 1316 |
| SmiMI | CAYNNNNRTG | 7 cut(s) | 398, 480, 554, 639, 803, 924, 1686 |
| SmlI | CTYRAG | 1 cut(s) | 11 |
| SmoI | CTYRAG | 1 cut(s) | 11 |
| SphI | GCATGC | 2 cut(s) | 1396, 2206 |
| SsiI | CCGC | 7 cut(s) | 160, 353, 1399, 1934, 2000, 2081, 2260 |
| SspMI | CTAG | 2 cut(s) | 1329, 2284 |
| StyD4I | CCNGG | 6 cut(s) | 591, 1197, 1604, 1713, 1744, 1865 |
| StyI | CCWWGG | 2 cut(s) | 387, 956 |
| TaaI | ACNGT | 4 cut(s) | 491, 1094, 1641, 1728 |
| TaiI | ACGT | 1 cut(s) | 510 |
| TaqI | TCGA | 6 cut(s) | 510, 680, 1096, 1499, 2022, 2032 |
| TatI | WGTACW | 3 cut(s) | 402, 1723, 2044 |
| TfiI | GAWTC | 5 cut(s) | 32, 98, 625, 736, 1553 |
| Tru1I | TTAA | 6 cut(s) | 12, 420, 1478, 1643, 1731, 2136 |
| Tru9I | TTAA | 6 cut(s) | 12, 420, 1478, 1643, 1731, 2136 |
| TscAI | CASTG | 5 cut(s) | 184, 300, 810, 1471, 2185 |
| TseFI | GTSAC | 4 cut(s) | 182, 515, 643, 1379 |
| TseI | GCWGC | 6 cut(s) | 1622, 1889, 1892, 1919, 2120, 2216 |
| Tsp45I | GTSAC | 4 cut(s) | 182, 515, 643, 1379 |
| TspGWI | ACGGA | 1 cut(s) | 1319 |
| TspRI | CASTG | 5 cut(s) | 184, 300, 810, 1471, 2185 |
| Vha464I | CTTAAG | 1 cut(s) | 11 |
| VneI | GTGCAC | 1 cut(s) | 1696 |
| VpaK11BI | GGWCC | 4 cut(s) | 575, 1195, 1306, 1316 |
| VspI | ATTAAT | 1 cut(s) | 2136 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 2022 |
| XapI | RAATTY | 1 cut(s) | 453 |
| XceI | RCATGY | 3 cut(s) | 802, 1396, 2206 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 771, 1114 |
| XmnI | GAANNNNTTC | 1 cut(s) | 221 |
| XspI | CTAG | 2 cut(s) | 1329, 2284 |
| ZrmI | AGTACT | 1 cut(s) | 1725 |
| Zsp2I | ATGCAT | 1 cut(s) | 762 |