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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 980 |
| Acc65I | GGTACC | 1 cut(s) | 1565 |
| AccB1I | GGYRCC | 2 cut(s) | 4, 1565 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 971 |
| AccI | GTMKAC | 1 cut(s) | 20 |
| AciI | CCGC | 4 cut(s) | 254, 292, 460, 1282 |
| AcoI | YGGCCR | 3 cut(s) | 742, 1168, 1490 |
| AcsI | RAATTY | 1 cut(s) | 611 |
| AcuI | CTGAAG | 4 cut(s) | 57, 164, 663, 1329 |
| AcvI | CACGTG | 1 cut(s) | 593 |
| AcyI | GRCGYC | 1 cut(s) | 5 |
| AfaI | GTAC | 6 cut(s) | 563, 805, 855, 1368, 1418, 1567 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 312, 971, 1034 |
| AflIII | ACRYGT | 2 cut(s) | 391, 592 |
| AjnI | CCWGG | 2 cut(s) | 843, 1192 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1302, 1334 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 49, 81, 223, 255 |
| AluBI | AGCT | 9 cut(s) | 62, 71, 77, 148, 198, 559, 1043, 1047, 1115 |
| AluI | AGCT | 9 cut(s) | 62, 71, 77, 148, 198, 559, 1043, 1047, 1115 |
| Alw26I | GTCTC | 4 cut(s) | 569, 881, 1331, 1525 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1340 |
| AoxI | GGCC | 6 cut(s) | 139, 742, 927, 1150, 1168, 1490 |
| ApeKI | GCWGC | 3 cut(s) | 44, 587, 1352 |
| ApoI | RAATTY | 1 cut(s) | 611 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1140, 1172 |
| Asp718I | GGTACC | 1 cut(s) | 1565 |
| AspLEI | GCGC | 1 cut(s) | 7 |
| AspS9I | GGNCC | 3 cut(s) | 139, 927, 1495 |
| AsuHPI | GGTGA | 4 cut(s) | 782, 1210, 1252, 1515 |
| AsuNHI | GCTAGC | 1 cut(s) | 1043 |
| AvaII | GGWCC | 1 cut(s) | 1495 |
| BalI | TGGCCA | 1 cut(s) | 1170 |
| BanI | GGYRCC | 2 cut(s) | 4, 1565 |
| BbrPI | CACGTG | 1 cut(s) | 593 |
| BbvCI | CCTCAGC | 1 cut(s) | 366 |
| BbvI | GCAGC | 3 cut(s) | 31, 574, 1364 |
| BccI | CCATC | 3 cut(s) | 16, 1028, 1506 |
| BceAI | ACGGC | 2 cut(s) | 411, 885 |
| BciT130I | CCWGG | 2 cut(s) | 845, 1194 |
| BciVI | GTATCC | 1 cut(s) | 453 |
| BcoDI | GTCTC | 4 cut(s) | 569, 881, 1331, 1525 |
| BfaI | CTAG | 5 cut(s) | 687, 798, 1001, 1010, 1044 |
| BfoI | RGCGCY | 1 cut(s) | 8 |
| BfuAI | ACCTGC | 1 cut(s) | 980 |
| BfuI | GTATCC | 1 cut(s) | 453 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 509 |
| BisI | GCNGC | 4 cut(s) | 45, 255, 588, 1353 |
| BlpI | GCTNAGC | 1 cut(s) | 861 |
| BlsI | GCNGC | 4 cut(s) | 46, 256, 589, 1354 |
| BmcAI | AGTACT | 1 cut(s) | 855 |
| Bme1390I | CCNGG | 2 cut(s) | 845, 1194 |
| Bme18I | GGWCC | 1 cut(s) | 1495 |
| BmgT120I | GGNCC | 3 cut(s) | 139, 927, 1495 |
| BmiI | GGNNCC | 2 cut(s) | 6, 1567 |
| BmrFI | CCNGG | 2 cut(s) | 845, 1194 |
| BmsI | GCATC | 3 cut(s) | 56, 101, 1375 |
| BmtI | GCTAGC | 1 cut(s) | 1047 |
| BpmI | CTGGAG | 1 cut(s) | 699 |
| Bpu10I | CCTNAGC | 1 cut(s) | 366 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 861 |
| BsaAI | YACGTR | 1 cut(s) | 593 |
| BsaBI | GATNNNNATC | 1 cut(s) | 836 |
| BsaHI | GRCGYC | 1 cut(s) | 5 |
| BsaJI | CCNNGG | 4 cut(s) | 26, 1193, 1356, 1470 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 223, 253 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 312, 971, 1034 |
| Bse118I | RCCGGY | 2 cut(s) | 508, 761 |
| Bse1I | ACTGG | 5 cut(s) | 682, 861, 965, 1138, 1380 |
| Bse3DI | GCAATG | 2 cut(s) | 177, 1239 |
| Bse8I | GATNNNNATC | 1 cut(s) | 836 |
| BseBI | CCWGG | 2 cut(s) | 845, 1194 |
| BseDI | CCNNGG | 4 cut(s) | 26, 1193, 1356, 1470 |
| BseGI | GGATG | 4 cut(s) | 8, 438, 553, 1020 |
| BseJI | GATNNNNATC | 1 cut(s) | 836 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 312, 971, 1034 |
| BseMI | GCAATG | 2 cut(s) | 177, 1239 |
| BseMII | CTCAG | 3 cut(s) | 357, 852, 1553 |
| BseNI | ACTGG | 5 cut(s) | 682, 861, 965, 1138, 1380 |
| BseRI | GAGGAG | 2 cut(s) | 137, 1291 |
| BseXI | GCAGC | 3 cut(s) | 31, 574, 1364 |
| BsgI | GTGCAG | 2 cut(s) | 416, 573 |
| BshFI | GGCC | 6 cut(s) | 141, 744, 929, 1152, 1170, 1492 |
| BshNI | GGYRCC | 2 cut(s) | 4, 1565 |
| BsiSI | CCGG | 5 cut(s) | 295, 509, 524, 762, 1493 |
| BslFI | GGGAC | 3 cut(s) | 250, 442, 996 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 312, 971, 1034 |
| BsmAI | GTCTC | 4 cut(s) | 569, 881, 1331, 1525 |
| BsmFI | GGGAC | 3 cut(s) | 250, 442, 996 |
| BsnI | GGCC | 6 cut(s) | 141, 744, 929, 1152, 1170, 1492 |
| Bsp143I | GATC | 2 cut(s) | 876, 1063 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 861 |
| Bsp19I | CCATGG | 1 cut(s) | 1470 |
| BspACI | CCGC | 4 cut(s) | 254, 292, 460, 1282 |
| BspANI | GGCC | 6 cut(s) | 141, 744, 929, 1152, 1170, 1492 |
| BspCNI | CTCAG | 3 cut(s) | 358, 853, 1552 |
| BspHI | TCATGA | 1 cut(s) | 118 |
| BspLI | GGNNCC | 2 cut(s) | 6, 1567 |
| BspMI | ACCTGC | 1 cut(s) | 980 |
| BspOI | GCTAGC | 1 cut(s) | 1047 |
| BspT107I | GGYRCC | 2 cut(s) | 4, 1565 |
| BsrDI | GCAATG | 2 cut(s) | 177, 1239 |
| BsrFI | RCCGGY | 2 cut(s) | 508, 761 |
| BsrI | ACTGG | 5 cut(s) | 682, 861, 965, 1138, 1380 |
| BssAI | RCCGGY | 2 cut(s) | 508, 761 |
| BssECI | CCNNGG | 4 cut(s) | 26, 1193, 1356, 1470 |
| BssMI | GATC | 2 cut(s) | 876, 1063 |
| BssNI | GRCGYC | 1 cut(s) | 5 |
| BssT1I | CCWWGG | 2 cut(s) | 26, 1470 |
| Bst2UI | CCWGG | 2 cut(s) | 845, 1194 |
| Bst4CI | ACNGT | 3 cut(s) | 957, 1317, 1459 |
| Bst6I | CTCTTC | 6 cut(s) | 170, 203, 522, 639, 879, 1541 |
| BstACI | GRCGYC | 1 cut(s) | 5 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 403 |
| BstBAI | YACGTR | 1 cut(s) | 593 |
| BstC8I | GCNNGC | 7 cut(s) | 292, 424, 510, 763, 1041, 1045, 1098 |
| BstDEI | CTNAG | 5 cut(s) | 286, 366, 692, 861, 1539 |
| BstDSI | CCRYGG | 1 cut(s) | 1470 |
| BstF5I | GGATG | 4 cut(s) | 8, 438, 553, 1020 |
| BstH2I | RGCGCY | 1 cut(s) | 8 |
| BstHHI | GCGC | 1 cut(s) | 7 |
| BstKTI | GATC | 2 cut(s) | 879, 1066 |
| BstMAI | GTCTC | 4 cut(s) | 569, 881, 1331, 1525 |
| BstMBI | GATC | 2 cut(s) | 876, 1063 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 68, 154, 403, 509, 816, 926, 1106 |
| BstNI | CCWGG | 2 cut(s) | 845, 1194 |
| BstSCI | CCNGG | 2 cut(s) | 843, 1192 |
| BstV1I | GCAGC | 3 cut(s) | 31, 574, 1364 |
| BsuI | GTATCC | 1 cut(s) | 453 |
| BsuRI | GGCC | 6 cut(s) | 141, 744, 929, 1152, 1170, 1492 |
| BtgI | CCRYGG | 1 cut(s) | 1470 |
| BtgZI | GCGATG | 1 cut(s) | 1458 |
| BtsCI | GGATG | 4 cut(s) | 8, 438, 553, 1020 |
| BtsI | GCAGTG | 1 cut(s) | 87 |
| BtsIMutI | CAGTG | 4 cut(s) | 87, 1059, 1131, 1387 |
| BveI | ACCTGC | 1 cut(s) | 980 |
| Cac8I | GCNNGC | 7 cut(s) | 292, 424, 510, 763, 1041, 1045, 1098 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1340 |
| CciI | TCATGA | 1 cut(s) | 118 |
| CfoI | GCGC | 1 cut(s) | 7 |
| Cfr10I | RCCGGY | 2 cut(s) | 508, 761 |
| Cfr13I | GGNCC | 3 cut(s) | 139, 927, 1495 |
| Csp6I | GTAC | 6 cut(s) | 562, 804, 854, 1367, 1417, 1566 |
| CviAII | CATG | 7 cut(s) | 119, 579, 737, 931, 1085, 1471, 1488 |
| CviQI | GTAC | 6 cut(s) | 562, 804, 854, 1367, 1417, 1566 |
| DdeI | CTNAG | 5 cut(s) | 286, 366, 692, 861, 1539 |
| DinI | GGCGCC | 1 cut(s) | 6 |
| DpnI | GATC | 2 cut(s) | 878, 1065 |
| DpnII | GATC | 2 cut(s) | 876, 1063 |
| EaeI | YGGCCR | 3 cut(s) | 742, 1168, 1490 |
| Eam1104I | CTCTTC | 6 cut(s) | 170, 203, 522, 639, 879, 1541 |
| EarI | CTCTTC | 6 cut(s) | 170, 203, 522, 639, 879, 1541 |
| Eco130I | CCWWGG | 2 cut(s) | 26, 1470 |
| Eco47I | GGWCC | 1 cut(s) | 1495 |
| Eco57I | CTGAAG | 4 cut(s) | 57, 164, 663, 1329 |
| Eco72I | CACGTG | 1 cut(s) | 593 |
| EcoO109I | RGGNCCY | 1 cut(s) | 139 |
| EcoRII | CCWGG | 2 cut(s) | 843, 1192 |
| EcoT14I | CCWWGG | 2 cut(s) | 26, 1470 |
| EcoT22I | ATGCAT | 2 cut(s) | 922, 1086 |
| EgeI | GGCGCC | 1 cut(s) | 6 |
| EheI | GGCGCC | 1 cut(s) | 6 |
| ErhI | CCWWGG | 2 cut(s) | 26, 1470 |
| FaeI | CATG | 7 cut(s) | 122, 582, 740, 934, 1088, 1474, 1491 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 1193, 1225, 1353, 1385 |
| FaqI | GGGAC | 3 cut(s) | 250, 442, 996 |
| FatI | CATG | 7 cut(s) | 118, 578, 736, 930, 1084, 1470, 1487 |
| FauI | CCCGC | 2 cut(s) | 299, 467 |
| FauNDI | CATATG | 1 cut(s) | 244 |
| FblI | GTMKAC | 1 cut(s) | 20 |
| Fnu4HI | GCNGC | 4 cut(s) | 45, 255, 588, 1353 |
| FokI | GGATG | 3 cut(s) | 445, 560, 1007 |
| Fsp4HI | GCNGC | 4 cut(s) | 45, 255, 588, 1353 |
| FspBI | CTAG | 5 cut(s) | 687, 798, 1001, 1010, 1044 |
| GlaI | GCGC | 1 cut(s) | 6 |
| GluI | GCNGC | 4 cut(s) | 45, 255, 588, 1353 |
| GsuI | CTGGAG | 1 cut(s) | 699 |
| HaeII | RGCGCY | 1 cut(s) | 8 |
| HaeIII | GGCC | 6 cut(s) | 141, 744, 929, 1152, 1170, 1492 |
| HapII | CCGG | 5 cut(s) | 295, 509, 524, 762, 1493 |
| HhaI | GCGC | 1 cut(s) | 7 |
| Hin1I | GRCGYC | 1 cut(s) | 5 |
| Hin1II | CATG | 7 cut(s) | 122, 582, 740, 934, 1088, 1474, 1491 |
| Hin6I | GCGC | 1 cut(s) | 5 |
| HinP1I | GCGC | 1 cut(s) | 5 |
| HincII | GTYRAC | 2 cut(s) | 772, 1206 |
| HindII | GTYRAC | 2 cut(s) | 772, 1206 |
| HindIII | AAGCTT | 1 cut(s) | 196 |
| HinfI | GANTC | 4 cut(s) | 128, 238, 301, 412 |
| HpaII | CCGG | 5 cut(s) | 295, 509, 524, 762, 1493 |
| HphI | GGTGA | 4 cut(s) | 782, 1210, 1252, 1515 |
| Hpy166II | GTNNAC | 5 cut(s) | 21, 772, 1206, 1243, 1299 |
| Hpy188I | TCNGA | 3 cut(s) | 133, 1374, 1542 |
| Hpy188III | TCNNGA | 5 cut(s) | 119, 186, 1226, 1325, 1574 |
| Hpy8I | GTNNAC | 5 cut(s) | 21, 772, 1206, 1243, 1299 |
| HpyAV | CCTTC | 9 cut(s) | 34, 244, 487, 631, 655, 661, 712, 724, 1172 |
| HpyCH4III | ACNGT | 3 cut(s) | 957, 1317, 1459 |
| HpyCH4IV | ACGT | 3 cut(s) | 391, 471, 592 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 68, 154, 403, 509, 816, 926, 1106 |
| HpyF3I | CTNAG | 5 cut(s) | 286, 366, 692, 861, 1539 |
| HpySE526I | ACGT | 3 cut(s) | 391, 471, 592 |
| Hsp92I | GRCGYC | 1 cut(s) | 5 |
| Hsp92II | CATG | 7 cut(s) | 122, 582, 740, 934, 1088, 1474, 1491 |
| HspAI | GCGC | 1 cut(s) | 5 |
| KasI | GGCGCC | 1 cut(s) | 4 |
| KpnI | GGTACC | 1 cut(s) | 1569 |
| KroI | GCCGGC | 2 cut(s) | 508, 761 |
| KroNI | GCCGGC | 2 cut(s) | 510, 763 |
| Kzo9I | GATC | 2 cut(s) | 876, 1063 |
| LmnI | GCTCC | 2 cut(s) | 733, 1531 |
| Lsp1109I | GCAGC | 3 cut(s) | 31, 574, 1364 |
| LweI | GCATC | 3 cut(s) | 56, 101, 1375 |
| MaeI | CTAG | 5 cut(s) | 687, 798, 1001, 1010, 1044 |
| MaeII | ACGT | 3 cut(s) | 391, 471, 592 |
| MaeIII | GTNAC | 4 cut(s) | 355, 595, 951, 1129 |
| MalI | GATC | 2 cut(s) | 878, 1065 |
| MboI | GATC | 2 cut(s) | 876, 1063 |
| MfeI | CAATTG | 2 cut(s) | 452, 903 |
| MlsI | TGGCCA | 1 cut(s) | 1170 |
| MluCI | AATT | 9 cut(s) | 160, 401, 452, 611, 651, 903, 1119, 1212, 1398 |
| MluNI | TGGCCA | 1 cut(s) | 1170 |
| Mly113I | GGCGCC | 1 cut(s) | 5 |
| MlyI | GAGTC | 2 cut(s) | 232, 406 |
| MmeI | TCCRAC | 2 cut(s) | 985, 1460 |
| Mox20I | TGGCCA | 1 cut(s) | 1170 |
| Mph1103I | ATGCAT | 2 cut(s) | 922, 1086 |
| MroNI | GCCGGC | 2 cut(s) | 508, 761 |
| MscI | TGGCCA | 1 cut(s) | 1170 |
| MseI | TTAA | 5 cut(s) | 200, 834, 969, 1211, 1401 |
| Msp20I | TGGCCA | 1 cut(s) | 1170 |
| MspA1I | CMGCKG | 1 cut(s) | 62 |
| MspI | CCGG | 5 cut(s) | 295, 509, 524, 762, 1493 |
| MspR9I | CCNGG | 2 cut(s) | 845, 1194 |
| MunI | CAATTG | 2 cut(s) | 452, 903 |
| MvaI | CCWGG | 2 cut(s) | 845, 1194 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 68, 154, 403, 509, 816, 926, 1106 |
| NaeI | GCCGGC | 2 cut(s) | 510, 763 |
| NarI | GGCGCC | 1 cut(s) | 5 |
| NcoI | CCATGG | 1 cut(s) | 1470 |
| NdeI | CATATG | 1 cut(s) | 244 |
| NdeII | GATC | 2 cut(s) | 876, 1063 |
| NgoMIV | GCCGGC | 2 cut(s) | 508, 761 |
| NheI | GCTAGC | 1 cut(s) | 1043 |
| NlaIII | CATG | 7 cut(s) | 122, 582, 740, 934, 1088, 1474, 1491 |
| NlaIV | GGNNCC | 2 cut(s) | 6, 1567 |
| NmeAIII | GCCGAG | 4 cut(s) | 529, 544, 770, 1381 |
| NmuCI | GTSAC | 2 cut(s) | 951, 1129 |
| NsiI | ATGCAT | 2 cut(s) | 922, 1086 |
| PagI | TCATGA | 1 cut(s) | 118 |
| PdiI | GCCGGC | 2 cut(s) | 510, 763 |
| PfeI | GAWTC | 2 cut(s) | 128, 301 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 971 |
| PkrI | GCNGC | 4 cut(s) | 46, 256, 589, 1354 |
| PleI | GAGTC | 2 cut(s) | 232, 406 |
| PluTI | GGCGCC | 1 cut(s) | 8 |
| PmaCI | CACGTG | 1 cut(s) | 593 |
| PmlI | CACGTG | 1 cut(s) | 593 |
| PpsI | GAGTC | 2 cut(s) | 232, 406 |
| Ppu21I | YACGTR | 1 cut(s) | 593 |
| Psp6I | CCWGG | 2 cut(s) | 843, 1192 |
| PspCI | CACGTG | 1 cut(s) | 593 |
| PspGI | CCWGG | 2 cut(s) | 843, 1192 |
| PspN4I | GGNNCC | 2 cut(s) | 6, 1567 |
| PspPI | GGNCC | 3 cut(s) | 139, 927, 1495 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1340 |
| PvuII | CAGCTG | 1 cut(s) | 62 |
| RsaI | GTAC | 6 cut(s) | 563, 805, 855, 1368, 1418, 1567 |
| RsaNI | GTAC | 6 cut(s) | 562, 804, 854, 1367, 1417, 1566 |
| SaqAI | TTAA | 5 cut(s) | 200, 834, 969, 1211, 1401 |
| SatI | GCNGC | 4 cut(s) | 45, 255, 588, 1353 |
| Sau3AI | GATC | 2 cut(s) | 876, 1063 |
| Sau96I | GGNCC | 3 cut(s) | 139, 927, 1495 |
| ScaI | AGTACT | 1 cut(s) | 855 |
| SchI | GAGTC | 2 cut(s) | 232, 406 |
| ScrFI | CCNGG | 2 cut(s) | 845, 1194 |
| SfaNI | GCATC | 3 cut(s) | 56, 101, 1375 |
| SfoI | GGCGCC | 1 cut(s) | 6 |
| SinI | GGWCC | 1 cut(s) | 1495 |
| Sse9I | AATT | 9 cut(s) | 160, 401, 452, 611, 651, 903, 1119, 1212, 1398 |
| SsiI | CCGC | 4 cut(s) | 254, 292, 460, 1282 |
| SspDI | GGCGCC | 1 cut(s) | 4 |
| SspMI | CTAG | 5 cut(s) | 687, 798, 1001, 1010, 1044 |
| StyD4I | CCNGG | 2 cut(s) | 843, 1192 |
| StyI | CCWWGG | 2 cut(s) | 26, 1470 |
| TaaI | ACNGT | 3 cut(s) | 957, 1317, 1459 |
| TaiI | ACGT | 3 cut(s) | 394, 474, 595 |
| TaqI | TCGA | 3 cut(s) | 95, 1158, 1573 |
| TasI | AATT | 9 cut(s) | 160, 401, 452, 611, 651, 903, 1119, 1212, 1398 |
| TatI | WGTACW | 2 cut(s) | 853, 1366 |
| TauI | GCSGC | 1 cut(s) | 257 |
| TfiI | GAWTC | 2 cut(s) | 128, 301 |
| Tru1I | TTAA | 5 cut(s) | 200, 834, 969, 1211, 1401 |
| Tru9I | TTAA | 5 cut(s) | 200, 834, 969, 1211, 1401 |
| TscAI | CASTG | 4 cut(s) | 94, 1066, 1138, 1387 |
| TseFI | GTSAC | 2 cut(s) | 951, 1129 |
| TseI | GCWGC | 3 cut(s) | 44, 587, 1352 |
| Tsp45I | GTSAC | 2 cut(s) | 951, 1129 |
| TspDTI | ATGAA | 6 cut(s) | 188, 281, 449, 564, 636, 947 |
| TspGWI | ACGGA | 1 cut(s) | 560 |
| TspRI | CASTG | 4 cut(s) | 94, 1066, 1138, 1387 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 971 |
| VpaK11BI | GGWCC | 1 cut(s) | 1495 |
| XapI | RAATTY | 1 cut(s) | 611 |
| XmiI | GTMKAC | 1 cut(s) | 20 |
| XspI | CTAG | 5 cut(s) | 687, 798, 1001, 1010, 1044 |
| ZrmI | AGTACT | 1 cut(s) | 855 |
| Zsp2I | ATGCAT | 2 cut(s) | 922, 1086 |