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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 2138 |
| AarI | CACCTGC | 1 cut(s) | 2323 |
| AatII | GACGTC | 1 cut(s) | 2509 |
| Acc36I | ACCTGC | 1 cut(s) | 2323 |
| AccB1I | GGYRCC | 1 cut(s) | 1742 |
| AccII | CGCG | 2 cut(s) | 91, 305 |
| AclWI | GGATC | 3 cut(s) | 408, 750, 2216 |
| AcoI | YGGCCR | 1 cut(s) | 967 |
| AcsI | RAATTY | 6 cut(s) | 140, 722, 786, 834, 1273, 1937 |
| AcuI | CTGAAG | 4 cut(s) | 822, 1598, 1626, 1977 |
| AcyI | GRCGYC | 3 cut(s) | 252, 2400, 2506 |
| AfaI | GTAC | 3 cut(s) | 1375, 1390, 2163 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 220, 261, 295, 2066, 2409, 2525 |
| AflIII | ACRYGT | 3 cut(s) | 303, 1588, 2158 |
| AjiI | CACGTC | 1 cut(s) | 1589 |
| AjnI | CCWGG | 6 cut(s) | 570, 969, 2059, 2114, 2313, 2430 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1749, 1781 |
| AleI | CACNNNNGTG | 2 cut(s) | 1029, 1737 |
| AloI | GAACNNNNNNTCC | 4 cut(s) | 1761, 1793, 1854, 1886 |
| Alw21I | GWGCWC | 4 cut(s) | 744, 1734, 1909, 2172 |
| Alw26I | GTCTC | 5 cut(s) | 448, 1782, 2235, 2359, 2494 |
| Alw44I | GTGCAC | 1 cut(s) | 1905 |
| AlwI | GGATC | 3 cut(s) | 408, 750, 2216 |
| Ama87I | CYCGRG | 2 cut(s) | 215, 290 |
| AoxI | GGCC | 4 cut(s) | 36, 967, 972, 2428 |
| ApaI | GGGCCC | 1 cut(s) | 40 |
| ApaLI | GTGCAC | 1 cut(s) | 1905 |
| ApeKI | GCWGC | 6 cut(s) | 698, 1324, 1547, 1562, 1565, 1700 |
| ApoI | RAATTY | 6 cut(s) | 140, 722, 786, 834, 1273, 1937 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 1083, 1115 |
| AseI | ATTAAT | 1 cut(s) | 1671 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 2068 |
| AspLEI | GCGC | 1 cut(s) | 1224 |
| AspS9I | GGNCC | 6 cut(s) | 36, 37, 286, 845, 871, 2428 |
| AsuHPI | GGTGA | 7 cut(s) | 545, 901, 1483, 1686, 1688, 2100, 2347 |
| AsuII | TTCGAA | 1 cut(s) | 1557 |
| AvaI | CYCGRG | 2 cut(s) | 215, 290 |
| AvaII | GGWCC | 3 cut(s) | 286, 845, 871 |
| AxyI | CCTNAGG | 1 cut(s) | 474 |
| BaeGI | GKGCMC | 2 cut(s) | 40, 1909 |
| BanI | GGYRCC | 1 cut(s) | 1742 |
| BanII | GRGCYC | 1 cut(s) | 40 |
| BbsI | GAAGAC | 1 cut(s) | 357 |
| Bbv12I | GWGCWC | 4 cut(s) | 744, 1734, 1909, 2172 |
| BbvI | GCAGC | 6 cut(s) | 710, 1336, 1534, 1549, 1577, 1687 |
| BccI | CCATC | 7 cut(s) | 214, 542, 1090, 2023, 2204, 2459, 2468 |
| BceAI | ACGGC | 1 cut(s) | 1316 |
| BciT130I | CCWGG | 6 cut(s) | 572, 971, 2061, 2116, 2315, 2432 |
| BcoDI | GTCTC | 5 cut(s) | 448, 1782, 2235, 2359, 2494 |
| BfaI | CTAG | 5 cut(s) | 56, 540, 1785, 1890, 2459 |
| BfmI | CTRYAG | 2 cut(s) | 1563, 1920 |
| BfuAI | ACCTGC | 1 cut(s) | 2323 |
| BglII | AGATCT | 2 cut(s) | 682, 1988 |
| BisI | GCNGC | 7 cut(s) | 699, 1325, 1548, 1563, 1566, 1701, 2358 |
| BlsI | GCNGC | 7 cut(s) | 700, 1326, 1549, 1564, 1567, 1702, 2359 |
| BmcAI | AGTACT | 1 cut(s) | 1390 |
| Bme1390I | CCNGG | 6 cut(s) | 572, 971, 2061, 2116, 2315, 2432 |
| Bme18I | GGWCC | 3 cut(s) | 286, 845, 871 |
| BmeT110I | CYCGRG | 2 cut(s) | 215, 290 |
| BmgBI | CACGTC | 1 cut(s) | 1589 |
| BmgT120I | GGNCC | 6 cut(s) | 36, 37, 286, 845, 871, 2428 |
| BmiI | GGNNCC | 6 cut(s) | 38, 85, 365, 873, 923, 1744 |
| BmrFI | CCNGG | 6 cut(s) | 572, 971, 2061, 2116, 2315, 2432 |
| BmsI | GCATC | 9 cut(s) | 771, 882, 1030, 1186, 1252, 1428, 1610, 1649, 1933 |
| BoxI | GACNNNNGTC | 1 cut(s) | 549 |
| BpiI | GAAGAC | 1 cut(s) | 357 |
| BplI | GAGNNNNNCTC | 4 cut(s) | 2168, 2200, 2231, 2263 |
| BpmI | CTGGAG | 3 cut(s) | 1090, 1881, 2324 |
| Bpu10I | CCTNAGC | 1 cut(s) | 2166 |
| Bpu14I | TTCGAA | 1 cut(s) | 1557 |
| BpuEI | CTTGAG | 3 cut(s) | 671, 961, 2233 |
| Bsa29I | ATCGAT | 3 cut(s) | 194, 754, 1630 |
| BsaHI | GRCGYC | 3 cut(s) | 252, 2400, 2506 |
| BsaI | GGTCTC | 1 cut(s) | 448 |
| BsaJI | CCNNGG | 9 cut(s) | 214, 231, 414, 438, 570, 763, 2313, 2394, 2431 |
| BsaWI | WCCGGW | 1 cut(s) | 1340 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1854, 1884, 2491, 2521 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 220, 261, 295, 2066, 2409, 2525 |
| Bse118I | RCCGGY | 2 cut(s) | 28, 965 |
| Bse1I | ACTGG | 5 cut(s) | 367, 1073, 1396, 1864, 2307 |
| Bse21I | CCTNAGG | 1 cut(s) | 474 |
| BseBI | CCWGG | 6 cut(s) | 572, 971, 2061, 2116, 2315, 2432 |
| BseCI | ATCGAT | 3 cut(s) | 194, 754, 1630 |
| BseDI | CCNNGG | 9 cut(s) | 214, 231, 414, 438, 570, 763, 2313, 2394, 2431 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 220, 261, 295, 2066, 2409, 2525 |
| BseMII | CTCAG | 8 cut(s) | 191, 488, 597, 1260, 1334, 2034, 2157, 2250 |
| BseNI | ACTGG | 5 cut(s) | 367, 1073, 1396, 1864, 2307 |
| BseRI | GAGGAG | 5 cut(s) | 76, 340, 494, 686, 1733 |
| BseSI | GKGCMC | 2 cut(s) | 40, 1909 |
| BseXI | GCAGC | 6 cut(s) | 710, 1336, 1534, 1549, 1577, 1687 |
| BseYI | CCCAGC | 1 cut(s) | 2408 |
| BsgI | GTGCAG | 1 cut(s) | 1671 |
| Bsh1236I | CGCG | 2 cut(s) | 91, 305 |
| BshFI | GGCC | 4 cut(s) | 38, 969, 974, 2430 |
| BshNI | GGYRCC | 1 cut(s) | 1742 |
| BshVI | ATCGAT | 3 cut(s) | 194, 754, 1630 |
| BsiHKAI | GWGCWC | 4 cut(s) | 744, 1734, 1909, 2172 |
| BsiHKCI | CYCGRG | 2 cut(s) | 215, 290 |
| BsiSI | CCGG | 3 cut(s) | 29, 966, 1341 |
| BslFI | GGGAC | 2 cut(s) | 1599, 1930 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 220, 261, 295, 2066, 2409, 2525 |
| BsmAI | GTCTC | 5 cut(s) | 448, 1782, 2235, 2359, 2494 |
| BsmBI | CGTCTC | 1 cut(s) | 2494 |
| BsmFI | GGGAC | 2 cut(s) | 1599, 1930 |
| BsmI | GAATGC | 3 cut(s) | 1066, 1483, 1549 |
| BsnI | GGCC | 4 cut(s) | 38, 969, 974, 2430 |
| Bso31I | GGTCTC | 1 cut(s) | 448 |
| BsoBI | CYCGRG | 2 cut(s) | 215, 290 |
| Bsp119I | TTCGAA | 1 cut(s) | 1557 |
| Bsp120I | GGGCCC | 1 cut(s) | 36 |
| Bsp1286I | GDGCHC | 5 cut(s) | 40, 744, 1734, 1909, 2172 |
| Bsp143I | GATC | 8 cut(s) | 400, 682, 755, 904, 1627, 1631, 1988, 2221 |
| Bsp19I | CCATGG | 1 cut(s) | 763 |
| Bsp68I | TCGCGA | 1 cut(s) | 91 |
| BspANI | GGCC | 4 cut(s) | 38, 969, 974, 2430 |
| BspCNI | CTCAG | 8 cut(s) | 190, 487, 596, 1261, 1333, 2033, 2158, 2249 |
| BspDI | ATCGAT | 3 cut(s) | 194, 754, 1630 |
| BspFNI | CGCG | 2 cut(s) | 91, 305 |
| BspLI | GGNNCC | 6 cut(s) | 38, 85, 365, 873, 923, 1744 |
| BspMAI | CTGCAG | 2 cut(s) | 1567, 1924 |
| BspMI | ACCTGC | 1 cut(s) | 2323 |
| BspPI | GGATC | 3 cut(s) | 408, 750, 2216 |
| BspT104I | TTCGAA | 1 cut(s) | 1557 |
| BspT107I | GGYRCC | 1 cut(s) | 1742 |
| BspTNI | GGTCTC | 1 cut(s) | 448 |
| BsrFI | RCCGGY | 2 cut(s) | 28, 965 |
| BsrI | ACTGG | 5 cut(s) | 367, 1073, 1396, 1864, 2307 |
| BssAI | RCCGGY | 2 cut(s) | 28, 965 |
| BssECI | CCNNGG | 9 cut(s) | 214, 231, 414, 438, 570, 763, 2313, 2394, 2431 |
| BssMI | GATC | 8 cut(s) | 400, 682, 755, 904, 1627, 1631, 1988, 2221 |
| BssNI | GRCGYC | 3 cut(s) | 252, 2400, 2506 |
| BssT1I | CCWWGG | 5 cut(s) | 231, 414, 438, 763, 2394 |
| Bst2UI | CCWGG | 6 cut(s) | 572, 971, 2061, 2116, 2315, 2432 |
| Bst4CI | ACNGT | 2 cut(s) | 1870, 2104 |
| BstACI | GRCGYC | 3 cut(s) | 252, 2400, 2506 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1537 |
| BstBI | TTCGAA | 1 cut(s) | 1557 |
| BstC8I | GCNNGC | 7 cut(s) | 30, 211, 470, 696, 1241, 1329, 2330 |
| BstDSI | CCRYGG | 1 cut(s) | 763 |
| BstEII | GGTNACC | 1 cut(s) | 551 |
| BstFNI | CGCG | 2 cut(s) | 91, 305 |
| BstHHI | GCGC | 1 cut(s) | 1224 |
| BstKTI | GATC | 8 cut(s) | 403, 685, 758, 907, 1630, 1634, 1991, 2224 |
| BstMAI | GTCTC | 5 cut(s) | 448, 1782, 2235, 2359, 2494 |
| BstMBI | GATC | 8 cut(s) | 400, 682, 755, 904, 1627, 1631, 1988, 2221 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 19, 520, 529, 1537, 1885, 1904, 2399, 2427, 2519 |
| BstNI | CCWGG | 6 cut(s) | 572, 971, 2061, 2116, 2315, 2432 |
| BstNSI | RCATGY | 3 cut(s) | 527, 1243, 2162 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 549 |
| BstPI | GGTNACC | 1 cut(s) | 551 |
| BstSCI | CCNGG | 6 cut(s) | 570, 969, 2059, 2114, 2313, 2430 |
| BstSFI | CTRYAG | 2 cut(s) | 1563, 1920 |
| BstSLI | GKGCMC | 2 cut(s) | 40, 1909 |
| BstUI | CGCG | 2 cut(s) | 91, 305 |
| BstV1I | GCAGC | 6 cut(s) | 710, 1336, 1534, 1549, 1577, 1687 |
| BstV2I | GAAGAC | 1 cut(s) | 357 |
| BstX2I | RGATCY | 2 cut(s) | 682, 1988 |
| BstXI | CCANNNNNNTGG | 5 cut(s) | 238, 1096, 1292, 2302, 2321 |
| BstYI | RGATCY | 2 cut(s) | 682, 1988 |
| Bsu15I | ATCGAT | 3 cut(s) | 194, 754, 1630 |
| Bsu36I | CCTNAGG | 1 cut(s) | 474 |
| BsuRI | GGCC | 4 cut(s) | 38, 969, 974, 2430 |
| BsuTUI | ATCGAT | 3 cut(s) | 194, 754, 1630 |
| BtgI | CCRYGG | 1 cut(s) | 763 |
| BtrI | CACGTC | 1 cut(s) | 1589 |
| BtsIMutI | CAGTG | 3 cut(s) | 1266, 1630, 1909 |
| BtuMI | TCGCGA | 1 cut(s) | 91 |
| BveI | ACCTGC | 1 cut(s) | 2323 |
| Cac8I | GCNNGC | 7 cut(s) | 30, 211, 470, 696, 1241, 1329, 2330 |
| CfoI | GCGC | 1 cut(s) | 1224 |
| Cfr10I | RCCGGY | 2 cut(s) | 28, 965 |
| Cfr13I | GGNCC | 6 cut(s) | 36, 37, 286, 845, 871, 2428 |
| ClaI | ATCGAT | 3 cut(s) | 194, 754, 1630 |
| CseI | GACGC | 6 cut(s) | 260, 294, 311, 545, 1510, 2408 |
| Csp6I | GTAC | 3 cut(s) | 1374, 1389, 2162 |
| CviQI | GTAC | 3 cut(s) | 1374, 1389, 2162 |
| DpnI | GATC | 8 cut(s) | 402, 684, 757, 906, 1629, 1633, 1990, 2223 |
| DpnII | GATC | 8 cut(s) | 400, 682, 755, 904, 1627, 1631, 1988, 2221 |
| EaeI | YGGCCR | 1 cut(s) | 967 |
| EciI | GGCGGA | 5 cut(s) | 9, 1067, 1695, 2134, 2259 |
| Eco130I | CCWWGG | 5 cut(s) | 231, 414, 438, 763, 2394 |
| Eco147I | AGGCCT | 1 cut(s) | 974 |
| Eco24I | GRGCYC | 1 cut(s) | 40 |
| Eco31I | GGTCTC | 1 cut(s) | 448 |
| Eco47I | GGWCC | 3 cut(s) | 286, 845, 871 |
| Eco57I | CTGAAG | 4 cut(s) | 822, 1598, 1626, 1977 |
| Eco81I | CCTNAGG | 1 cut(s) | 474 |
| Eco88I | CYCGRG | 2 cut(s) | 215, 290 |
| Eco91I | GGTNACC | 1 cut(s) | 551 |
| EcoO109I | RGGNCCY | 4 cut(s) | 36, 286, 871, 2428 |
| EcoO65I | GGTNACC | 1 cut(s) | 551 |
| EcoRI | GAATTC | 3 cut(s) | 140, 834, 1273 |
| EcoRII | CCWGG | 6 cut(s) | 570, 969, 2059, 2114, 2313, 2430 |
| EcoT14I | CCWWGG | 5 cut(s) | 231, 414, 438, 763, 2394 |
| EcoT22I | ATGCAT | 3 cut(s) | 897, 1241, 1245 |
| EcoT38I | GRGCYC | 1 cut(s) | 40 |
| ErhI | CCWWGG | 5 cut(s) | 231, 414, 438, 763, 2394 |
| Esp3I | CGTCTC | 1 cut(s) | 2494 |
| FalI | AAGNNNNNCTT | 5 cut(s) | 431, 463, 2249, 2281, 2527 |
| FaqI | GGGAC | 2 cut(s) | 1599, 1930 |
| FauI | CCCGC | 1 cut(s) | 1350 |
| Fnu4HI | GCNGC | 7 cut(s) | 699, 1325, 1548, 1563, 1566, 1701, 2358 |
| FriOI | GRGCYC | 1 cut(s) | 40 |
| Fsp4HI | GCNGC | 7 cut(s) | 699, 1325, 1548, 1563, 1566, 1701, 2358 |
| FspBI | CTAG | 5 cut(s) | 56, 540, 1785, 1890, 2459 |
| GlaI | GCGC | 1 cut(s) | 1223 |
| GluI | GCNGC | 7 cut(s) | 699, 1325, 1548, 1563, 1566, 1701, 2358 |
| GsaI | CCCAGC | 1 cut(s) | 2412 |
| GsuI | CTGGAG | 3 cut(s) | 1090, 1881, 2324 |
| HaeIII | GGCC | 4 cut(s) | 38, 969, 974, 2430 |
| HapII | CCGG | 3 cut(s) | 29, 966, 1341 |
| HgaI | GACGC | 6 cut(s) | 260, 294, 311, 545, 1510, 2408 |
| HhaI | GCGC | 1 cut(s) | 1224 |
| Hin1I | GRCGYC | 3 cut(s) | 252, 2400, 2506 |
| Hin6I | GCGC | 1 cut(s) | 1222 |
| HinP1I | GCGC | 1 cut(s) | 1222 |
| HincII | GTYRAC | 1 cut(s) | 499 |
| HindII | GTYRAC | 1 cut(s) | 499 |
| HindIII | AAGCTT | 4 cut(s) | 662, 827, 2251, 2511 |
| HinfI | GANTC | 9 cut(s) | 16, 93, 191, 1074, 1181, 1652, 1844, 2153, 2318 |
| HpaI | GTTAAC | 1 cut(s) | 499 |
| HpaII | CCGG | 3 cut(s) | 29, 966, 1341 |
| HphI | GGTGA | 7 cut(s) | 545, 901, 1483, 1686, 1688, 2100, 2347 |
| Hpy166II | GTNNAC | 7 cut(s) | 277, 301, 313, 499, 1216, 1725, 1907 |
| Hpy188III | TCNNGA | 9 cut(s) | 90, 105, 217, 292, 476, 590, 908, 1656, 2257 |
| Hpy8I | GTNNAC | 7 cut(s) | 277, 301, 313, 499, 1216, 1725, 1907 |
| Hpy99I | CGWCG | 7 cut(s) | 140, 208, 284, 310, 359, 539, 2508 |
| HpyCH4III | ACNGT | 2 cut(s) | 1870, 2104 |
| HpyCH4IV | ACGT | 3 cut(s) | 928, 1588, 2506 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 19, 520, 529, 1537, 1885, 1904, 2399, 2427, 2519 |
| HpySE526I | ACGT | 3 cut(s) | 928, 1588, 2506 |
| Hsp92I | GRCGYC | 3 cut(s) | 252, 2400, 2506 |
| HspAI | GCGC | 1 cut(s) | 1222 |
| KroI | GCCGGC | 1 cut(s) | 28 |
| KroNI | GCCGGC | 1 cut(s) | 30 |
| KspAI | GTTAAC | 1 cut(s) | 499 |
| Kzo9I | GATC | 8 cut(s) | 400, 682, 755, 904, 1627, 1631, 1988, 2221 |
| Lsp1109I | GCAGC | 6 cut(s) | 710, 1336, 1534, 1549, 1577, 1687 |
| LweI | GCATC | 9 cut(s) | 771, 882, 1030, 1186, 1252, 1428, 1610, 1649, 1933 |
| MaeI | CTAG | 5 cut(s) | 56, 540, 1785, 1890, 2459 |
| MaeII | ACGT | 3 cut(s) | 928, 1588, 2506 |
| MaeIII | GTNAC | 6 cut(s) | 542, 551, 1131, 1798, 1855, 2098 |
| MalI | GATC | 8 cut(s) | 402, 684, 757, 906, 1629, 1633, 1990, 2223 |
| MboI | GATC | 8 cut(s) | 400, 682, 755, 904, 1627, 1631, 1988, 2221 |
| MfeI | CAATTG | 1 cut(s) | 41 |
| MflI | RGATCY | 2 cut(s) | 682, 1988 |
| MhlI | GDGCHC | 5 cut(s) | 40, 744, 1734, 1909, 2172 |
| MluI | ACGCGT | 1 cut(s) | 303 |
| MlyI | GAGTC | 2 cut(s) | 10, 102 |
| MmeI | TCCRAC | 3 cut(s) | 949, 1747, 1956 |
| Mph1103I | ATGCAT | 3 cut(s) | 897, 1241, 1245 |
| MreI | CGCCGGCG | 1 cut(s) | 28 |
| MroNI | GCCGGC | 1 cut(s) | 28 |
| MroXI | GAANNNNTTC | 1 cut(s) | 2068 |
| MseI | TTAA | 9 cut(s) | 321, 486, 498, 717, 1148, 1314, 1460, 1671, 2551 |
| MslI | CAYNNNNRTG | 5 cut(s) | 1029, 1140, 1248, 1290, 1737 |
| MspA1I | CMGCKG | 1 cut(s) | 1700 |
| MspI | CCGG | 3 cut(s) | 29, 966, 1341 |
| MspR9I | CCNGG | 6 cut(s) | 572, 971, 2061, 2116, 2315, 2432 |
| MunI | CAATTG | 1 cut(s) | 41 |
| Mva1269I | GAATGC | 3 cut(s) | 1066, 1483, 1549 |
| MvaI | CCWGG | 6 cut(s) | 572, 971, 2061, 2116, 2315, 2432 |
| MvnI | CGCG | 2 cut(s) | 91, 305 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 19, 520, 529, 1537, 1885, 1904, 2399, 2427, 2519 |
| NaeI | GCCGGC | 1 cut(s) | 30 |
| NcoI | CCATGG | 1 cut(s) | 763 |
| NdeII | GATC | 8 cut(s) | 400, 682, 755, 904, 1627, 1631, 1988, 2221 |
| NgoMIV | GCCGGC | 1 cut(s) | 28 |
| NlaIV | GGNNCC | 6 cut(s) | 38, 85, 365, 873, 923, 1744 |
| NmuCI | GTSAC | 2 cut(s) | 542, 551 |
| NruI | TCGCGA | 1 cut(s) | 91 |
| NsiI | ATGCAT | 3 cut(s) | 897, 1241, 1245 |
| NspI | RCATGY | 3 cut(s) | 527, 1243, 2162 |
| NspV | TTCGAA | 1 cut(s) | 1557 |
| OliI | CACNNNNGTG | 2 cut(s) | 1029, 1737 |
| PaeI | GCATGC | 1 cut(s) | 1243 |
| PaeR7I | CTCGAG | 1 cut(s) | 290 |
| PaqCI | CACCTGC | 1 cut(s) | 2323 |
| PceI | AGGCCT | 1 cut(s) | 974 |
| PciI | ACATGT | 1 cut(s) | 2158 |
| PcsI | WCGNNNNNNNCGW | 3 cut(s) | 200, 288, 543 |
| PctI | GAATGC | 3 cut(s) | 1066, 1483, 1549 |
| PdiI | GCCGGC | 1 cut(s) | 30 |
| PdmI | GAANNNNTTC | 1 cut(s) | 2068 |
| PfeI | GAWTC | 7 cut(s) | 191, 1074, 1181, 1652, 1844, 2153, 2318 |
| PflFI | GACNNNGTC | 3 cut(s) | 284, 409, 2505 |
| PkrI | GCNGC | 7 cut(s) | 700, 1326, 1549, 1564, 1567, 1702, 2359 |
| PleI | GAGTC | 2 cut(s) | 10, 101 |
| PpsI | GAGTC | 2 cut(s) | 10, 101 |
| PpuMI | RGGWCCY | 2 cut(s) | 286, 871 |
| PscI | ACATGT | 1 cut(s) | 2158 |
| PshAI | GACNNNNGTC | 1 cut(s) | 549 |
| PshBI | ATTAAT | 1 cut(s) | 1671 |
| PsiI | TTATAA | 1 cut(s) | 2138 |
| Psp5II | RGGWCCY | 2 cut(s) | 286, 871 |
| Psp6I | CCWGG | 6 cut(s) | 570, 969, 2059, 2114, 2313, 2430 |
| PspEI | GGTNACC | 1 cut(s) | 551 |
| PspFI | CCCAGC | 1 cut(s) | 2408 |
| PspGI | CCWGG | 6 cut(s) | 570, 969, 2059, 2114, 2313, 2430 |
| PspN4I | GGNNCC | 6 cut(s) | 38, 85, 365, 873, 923, 1744 |
| PspOMI | GGGCCC | 1 cut(s) | 36 |
| PspPI | GGNCC | 6 cut(s) | 36, 37, 286, 845, 871, 2428 |
| PspPPI | RGGWCCY | 2 cut(s) | 286, 871 |
| PstI | CTGCAG | 2 cut(s) | 1567, 1924 |
| PsuI | RGATCY | 2 cut(s) | 682, 1988 |
| PsyI | GACNNNGTC | 3 cut(s) | 284, 409, 2505 |
| PvuII | CAGCTG | 1 cut(s) | 1700 |
| RruI | TCGCGA | 1 cut(s) | 91 |
| RsaI | GTAC | 3 cut(s) | 1375, 1390, 2163 |
| RsaNI | GTAC | 3 cut(s) | 1374, 1389, 2162 |
| RseI | CAYNNNNRTG | 5 cut(s) | 1029, 1140, 1248, 1290, 1737 |
| SaqAI | TTAA | 9 cut(s) | 321, 486, 498, 717, 1148, 1314, 1460, 1671, 2551 |
| SatI | GCNGC | 7 cut(s) | 699, 1325, 1548, 1563, 1566, 1701, 2358 |
| Sau3AI | GATC | 8 cut(s) | 400, 682, 755, 904, 1627, 1631, 1988, 2221 |
| Sau96I | GGNCC | 6 cut(s) | 36, 37, 286, 845, 871, 2428 |
| ScaI | AGTACT | 1 cut(s) | 1390 |
| SchI | GAGTC | 2 cut(s) | 10, 102 |
| ScrFI | CCNGG | 6 cut(s) | 572, 971, 2061, 2116, 2315, 2432 |
| SduI | GDGCHC | 5 cut(s) | 40, 744, 1734, 1909, 2172 |
| SfaNI | GCATC | 9 cut(s) | 771, 882, 1030, 1186, 1252, 1428, 1610, 1649, 1933 |
| SfcI | CTRYAG | 2 cut(s) | 1563, 1920 |
| Sfr274I | CTCGAG | 1 cut(s) | 290 |
| SfuI | TTCGAA | 1 cut(s) | 1557 |
| SgrAI | CRCCGGYG | 1 cut(s) | 28 |
| SinI | GGWCC | 3 cut(s) | 286, 845, 871 |
| SlaI | CTCGAG | 1 cut(s) | 290 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 1029, 1140, 1248, 1290, 1737 |
| SmlI | CTYRAG | 4 cut(s) | 290, 686, 976, 2248 |
| SmoI | CTYRAG | 4 cut(s) | 290, 686, 976, 2248 |
| SphI | GCATGC | 1 cut(s) | 1243 |
| SseBI | AGGCCT | 1 cut(s) | 974 |
| SspMI | CTAG | 5 cut(s) | 56, 540, 1785, 1890, 2459 |
| StuI | AGGCCT | 1 cut(s) | 974 |
| StyD4I | CCNGG | 6 cut(s) | 570, 969, 2059, 2114, 2313, 2430 |
| StyI | CCWWGG | 5 cut(s) | 231, 414, 438, 763, 2394 |
| TaaI | ACNGT | 2 cut(s) | 1870, 2104 |
| TaiI | ACGT | 3 cut(s) | 931, 1591, 2509 |
| TaqII | GACCGA | 1 cut(s) | 833 |
| TatI | WGTACW | 1 cut(s) | 1388 |
| TauI | GCSGC | 1 cut(s) | 2360 |
| TfiI | GAWTC | 7 cut(s) | 191, 1074, 1181, 1652, 1844, 2153, 2318 |
| Tru1I | TTAA | 9 cut(s) | 321, 486, 498, 717, 1148, 1314, 1460, 1671, 2551 |
| Tru9I | TTAA | 9 cut(s) | 321, 486, 498, 717, 1148, 1314, 1460, 1671, 2551 |
| TscAI | CASTG | 3 cut(s) | 1273, 1630, 1909 |
| TseFI | GTSAC | 2 cut(s) | 542, 551 |
| TseI | GCWGC | 6 cut(s) | 698, 1324, 1547, 1562, 1565, 1700 |
| Tsp45I | GTSAC | 2 cut(s) | 542, 551 |
| TspGWI | ACGGA | 2 cut(s) | 1749, 2537 |
| TspRI | CASTG | 3 cut(s) | 1273, 1630, 1909 |
| Tth111I | GACNNNGTC | 3 cut(s) | 284, 409, 2505 |
| VneI | GTGCAC | 1 cut(s) | 1905 |
| VpaK11BI | GGWCC | 3 cut(s) | 286, 845, 871 |
| VspI | ATTAAT | 1 cut(s) | 1671 |
| XapI | RAATTY | 6 cut(s) | 140, 722, 786, 834, 1273, 1937 |
| XceI | RCATGY | 3 cut(s) | 527, 1243, 2162 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 374, 932, 2321 |
| XhoI | CTCGAG | 1 cut(s) | 290 |
| XmnI | GAANNNNTTC | 1 cut(s) | 2068 |
| XspI | CTAG | 5 cut(s) | 56, 540, 1785, 1890, 2459 |
| ZraI | GACGTC | 1 cut(s) | 2507 |
| ZrmI | AGTACT | 1 cut(s) | 1390 |
| Zsp2I | ATGCAT | 3 cut(s) | 897, 1241, 1245 |