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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AluBI | AGCT | 1 cut(s) | 101 |
| AluI | AGCT | 1 cut(s) | 101 |
| CviAII | CATG | 1 cut(s) | 116 |
| CviJI | RGCY | 1 cut(s) | 101 |
| CviKI_1 | RGCY | 1 cut(s) | 101 |
| FaeI | CATG | 1 cut(s) | 119 |
| FaiI | YATR | 2 cut(s) | 117, 122 |
| FatI | CATG | 1 cut(s) | 115 |
| FspEI | CC | 8 cut(s) | 2, 14, 31, 32, 37, 65, 77, 91 |
| Hin1II | CATG | 1 cut(s) | 119 |
| HpyAV | CCTTC | 1 cut(s) | 73 |
| Hsp92II | CATG | 1 cut(s) | 119 |
| MnlI | CCTC | 1 cut(s) | 10 |
| NlaIII | CATG | 1 cut(s) | 119 |
| SetI | ASST | 1 cut(s) | 103 |
| SgeI | CNNG | 6 cut(s) | 34, 40, 55, 77, 110, 128 |
| TaqI | TCGA | 1 cut(s) | 97 |
| TspDTI | ATGAA | 1 cut(s) | 17 |