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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 1195 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 450 |
| AccI | GTMKAC | 2 cut(s) | 467, 1638 |
| AccII | CGCG | 1 cut(s) | 1302 |
| AccIII | TCCGGA | 1 cut(s) | 1890 |
| AciI | CCGC | 2 cut(s) | 1558, 1770 |
| AclWI | GGATC | 9 cut(s) | 313, 326, 938, 1023, 1574, 1607, 1620, 1805, 1818 |
| AcoI | YGGCCR | 1 cut(s) | 265 |
| AcsI | RAATTY | 1 cut(s) | 542 |
| AcuI | CTGAAG | 2 cut(s) | 1341, 1493 |
| AcyI | GRCGYC | 1 cut(s) | 1196 |
| AfaI | GTAC | 2 cut(s) | 242, 1388 |
| AfiI | CCNNNNNNNGG | 9 cut(s) | 328, 450, 507, 787, 1039, 1062, 1249, 1622, 1820 |
| AflII | CTTAAG | 1 cut(s) | 856 |
| AjnI | CCWGG | 5 cut(s) | 314, 694, 1552, 1621, 1851 |
| Alw21I | GWGCWC | 1 cut(s) | 188 |
| Alw26I | GTCTC | 4 cut(s) | 119, 631, 812, 1589 |
| AlwI | GGATC | 9 cut(s) | 313, 326, 938, 1023, 1574, 1607, 1620, 1805, 1818 |
| Ama87I | CYCGRG | 2 cut(s) | 686, 1698 |
| Aor13HI | TCCGGA | 1 cut(s) | 1890 |
| AoxI | GGCC | 6 cut(s) | 227, 265, 406, 682, 852, 1555 |
| ApaI | GGGCCC | 1 cut(s) | 686 |
| ApeKI | GCWGC | 4 cut(s) | 654, 1008, 1422, 1499 |
| ApoI | RAATTY | 1 cut(s) | 542 |
| ArsI | GACNNNNNNTTYG | 4 cut(s) | 380, 412, 661, 693 |
| AseI | ATTAAT | 1 cut(s) | 929 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 458 |
| AspLEI | GCGC | 3 cut(s) | 1198, 1302, 1788 |
| AspS9I | GGNCC | 3 cut(s) | 342, 682, 683 |
| AsuHPI | GGTGA | 4 cut(s) | 778, 1031, 1307, 1535 |
| AsuII | TTCGAA | 2 cut(s) | 74, 92 |
| AsuNHI | GCTAGC | 1 cut(s) | 1004 |
| AvaI | CYCGRG | 2 cut(s) | 686, 1698 |
| AvaII | GGWCC | 1 cut(s) | 342 |
| AxyI | CCTNAGG | 1 cut(s) | 1248 |
| BaeGI | GKGCMC | 1 cut(s) | 686 |
| BalI | TGGCCA | 1 cut(s) | 267 |
| BamHI | GGATCC | 3 cut(s) | 318, 1612, 1810 |
| BanI | GGYRCC | 1 cut(s) | 1195 |
| BanII | GRGCYC | 1 cut(s) | 686 |
| BauI | CACGAG | 1 cut(s) | 195 |
| BbsI | GAAGAC | 3 cut(s) | 63, 81, 1520 |
| Bbv12I | GWGCWC | 1 cut(s) | 188 |
| BbvI | GCAGC | 4 cut(s) | 666, 995, 1434, 1511 |
| BccI | CCATC | 4 cut(s) | 644, 823, 856, 1207 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 1225, 1259 |
| BciT130I | CCWGG | 5 cut(s) | 316, 696, 1554, 1623, 1853 |
| BcoDI | GTCTC | 4 cut(s) | 119, 631, 812, 1589 |
| BfaI | CTAG | 2 cut(s) | 870, 1005 |
| BfmI | CTRYAG | 1 cut(s) | 1449 |
| BfoI | RGCGCY | 1 cut(s) | 1199 |
| BfrI | CTTAAG | 1 cut(s) | 856 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 414 |
| BisI | GCNGC | 5 cut(s) | 655, 1009, 1423, 1500, 1558 |
| BlsI | GCNGC | 5 cut(s) | 656, 1010, 1424, 1501, 1559 |
| Bme1390I | CCNGG | 5 cut(s) | 316, 696, 1554, 1623, 1853 |
| Bme18I | GGWCC | 1 cut(s) | 342 |
| BmeT110I | CYCGRG | 2 cut(s) | 686, 1698 |
| BmgT120I | GGNCC | 3 cut(s) | 342, 682, 683 |
| BmiI | GGNNCC | 8 cut(s) | 34, 320, 344, 684, 685, 1197, 1614, 1812 |
| BmrFI | CCNGG | 5 cut(s) | 316, 696, 1554, 1623, 1853 |
| BmsI | GCATC | 5 cut(s) | 273, 556, 700, 1530, 1773 |
| BmtI | GCTAGC | 1 cut(s) | 1008 |
| BpiI | GAAGAC | 3 cut(s) | 63, 81, 1520 |
| BpmI | CTGGAG | 2 cut(s) | 298, 1035 |
| Bpu10I | CCTNAGC | 1 cut(s) | 837 |
| Bpu14I | TTCGAA | 2 cut(s) | 74, 92 |
| BpuEI | CTTGAG | 1 cut(s) | 45 |
| BsaHI | GRCGYC | 1 cut(s) | 1196 |
| BsaI | GGTCTC | 1 cut(s) | 1589 |
| BsaJI | CCNNGG | 6 cut(s) | 354, 409, 672, 731, 1818, 1842 |
| BsaWI | WCCGGW | 1 cut(s) | 1890 |
| BsaXI | ACNNNNNCTCC | 6 cut(s) | 71, 101, 1008, 1038, 1350, 1380 |
| Bsc4I | CCNNNNNNNGG | 9 cut(s) | 328, 450, 507, 787, 1039, 1062, 1249, 1622, 1820 |
| Bse1I | ACTGG | 2 cut(s) | 175, 1497 |
| Bse21I | CCTNAGG | 1 cut(s) | 1248 |
| Bse3DI | GCAATG | 2 cut(s) | 664, 1765 |
| BseAI | TCCGGA | 1 cut(s) | 1890 |
| BseBI | CCWGG | 5 cut(s) | 316, 696, 1554, 1623, 1853 |
| BseDI | CCNNGG | 6 cut(s) | 354, 409, 672, 731, 1818, 1842 |
| BseGI | GGATG | 4 cut(s) | 571, 691, 867, 1051 |
| BseLI | CCNNNNNNNGG | 9 cut(s) | 328, 450, 507, 787, 1039, 1062, 1249, 1622, 1820 |
| BseMI | GCAATG | 2 cut(s) | 664, 1765 |
| BseMII | CTCAG | 6 cut(s) | 114, 168, 885, 902, 1239, 1749 |
| BseNI | ACTGG | 2 cut(s) | 175, 1497 |
| BseRI | GAGGAG | 1 cut(s) | 48 |
| BseSI | GKGCMC | 1 cut(s) | 686 |
| BseXI | GCAGC | 4 cut(s) | 666, 995, 1434, 1511 |
| BseYI | CCCAGC | 1 cut(s) | 1754 |
| BsgI | GTGCAG | 1 cut(s) | 1441 |
| Bsh1236I | CGCG | 1 cut(s) | 1302 |
| Bsh1285I | CGRYCG | 1 cut(s) | 781 |
| BshFI | GGCC | 6 cut(s) | 229, 267, 408, 684, 854, 1557 |
| BshNI | GGYRCC | 1 cut(s) | 1195 |
| BsiEI | CGRYCG | 1 cut(s) | 781 |
| BsiHKAI | GWGCWC | 1 cut(s) | 188 |
| BsiHKCI | CYCGRG | 2 cut(s) | 686, 1698 |
| BsiSI | CCGG | 1 cut(s) | 1891 |
| BslFI | GGGAC | 2 cut(s) | 328, 1811 |
| BslI | CCNNNNNNNGG | 9 cut(s) | 328, 450, 507, 787, 1039, 1062, 1249, 1622, 1820 |
| BsmAI | GTCTC | 4 cut(s) | 119, 631, 812, 1589 |
| BsmFI | GGGAC | 2 cut(s) | 328, 1811 |
| BsmI | GAATGC | 1 cut(s) | 1772 |
| BsnI | GGCC | 6 cut(s) | 229, 267, 408, 684, 854, 1557 |
| Bso31I | GGTCTC | 1 cut(s) | 1589 |
| BsoBI | CYCGRG | 2 cut(s) | 686, 1698 |
| Bsp119I | TTCGAA | 2 cut(s) | 74, 92 |
| Bsp120I | GGGCCC | 1 cut(s) | 682 |
| Bsp1286I | GDGCHC | 2 cut(s) | 188, 686 |
| Bsp13I | TCCGGA | 1 cut(s) | 1890 |
| Bsp143I | GATC | 7 cut(s) | 318, 943, 1028, 1484, 1566, 1612, 1810 |
| Bsp19I | CCATGG | 1 cut(s) | 672 |
| BspACI | CCGC | 2 cut(s) | 1558, 1770 |
| BspANI | GGCC | 6 cut(s) | 229, 267, 408, 684, 854, 1557 |
| BspCNI | CTCAG | 6 cut(s) | 115, 167, 886, 903, 1240, 1748 |
| BspEI | TCCGGA | 1 cut(s) | 1890 |
| BspFNI | CGCG | 1 cut(s) | 1302 |
| BspHI | TCATGA | 2 cut(s) | 1536, 1791 |
| BspLI | GGNNCC | 8 cut(s) | 34, 320, 344, 684, 685, 1197, 1614, 1812 |
| BspOI | GCTAGC | 1 cut(s) | 1008 |
| BspPI | GGATC | 9 cut(s) | 313, 326, 938, 1023, 1574, 1607, 1620, 1805, 1818 |
| BspT104I | TTCGAA | 2 cut(s) | 74, 92 |
| BspT107I | GGYRCC | 1 cut(s) | 1195 |
| BspTI | CTTAAG | 1 cut(s) | 856 |
| BspTNI | GGTCTC | 1 cut(s) | 1589 |
| BsrDI | GCAATG | 2 cut(s) | 664, 1765 |
| BsrI | ACTGG | 2 cut(s) | 175, 1497 |
| BssECI | CCNNGG | 6 cut(s) | 354, 409, 672, 731, 1818, 1842 |
| BssMI | GATC | 7 cut(s) | 318, 943, 1028, 1484, 1566, 1612, 1810 |
| BssNI | GRCGYC | 1 cut(s) | 1196 |
| BssSI | CACGAG | 1 cut(s) | 195 |
| BssT1I | CCWWGG | 6 cut(s) | 354, 409, 672, 731, 1818, 1842 |
| Bst2BI | CACGAG | 1 cut(s) | 195 |
| Bst2UI | CCWGG | 5 cut(s) | 316, 696, 1554, 1623, 1853 |
| Bst4CI | ACNGT | 7 cut(s) | 782, 794, 1366, 1453, 1523, 1600, 1667 |
| Bst6I | CTCTTC | 6 cut(s) | 200, 810, 843, 1077, 1104, 1638 |
| BstACI | GRCGYC | 1 cut(s) | 1196 |
| BstAFI | CTTAAG | 1 cut(s) | 856 |
| BstBI | TTCGAA | 2 cut(s) | 74, 92 |
| BstC8I | GCNNGC | 4 cut(s) | 310, 953, 1006, 1342 |
| BstDEI | CTNAG | 9 cut(s) | 21, 123, 154, 224, 837, 894, 911, 1248, 1735 |
| BstDSI | CCRYGG | 1 cut(s) | 672 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 1247, 1620 |
| BstF5I | GGATG | 4 cut(s) | 571, 691, 867, 1051 |
| BstFNI | CGCG | 1 cut(s) | 1302 |
| BstH2I | RGCGCY | 1 cut(s) | 1199 |
| BstHHI | GCGC | 3 cut(s) | 1198, 1302, 1788 |
| BstKTI | GATC | 7 cut(s) | 321, 946, 1031, 1487, 1569, 1615, 1813 |
| BstMAI | GTCTC | 4 cut(s) | 119, 631, 812, 1589 |
| BstMBI | GATC | 7 cut(s) | 318, 943, 1028, 1484, 1566, 1612, 1810 |
| BstMCI | CGRYCG | 1 cut(s) | 781 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 414, 575, 1431 |
| BstNI | CCWGG | 5 cut(s) | 316, 696, 1554, 1623, 1853 |
| BstNSI | RCATGY | 1 cut(s) | 955 |
| BstSCI | CCNGG | 5 cut(s) | 314, 694, 1552, 1621, 1851 |
| BstSFI | CTRYAG | 1 cut(s) | 1449 |
| BstSLI | GKGCMC | 1 cut(s) | 686 |
| BstUI | CGCG | 1 cut(s) | 1302 |
| BstV1I | GCAGC | 4 cut(s) | 666, 995, 1434, 1511 |
| BstV2I | GAAGAC | 3 cut(s) | 63, 81, 1520 |
| BstX2I | RGATCY | 5 cut(s) | 318, 1028, 1566, 1612, 1810 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 679 |
| BstYI | RGATCY | 5 cut(s) | 318, 1028, 1566, 1612, 1810 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1248 |
| BsuRI | GGCC | 6 cut(s) | 229, 267, 408, 684, 854, 1557 |
| BtgI | CCRYGG | 1 cut(s) | 672 |
| BtsCI | GGATG | 4 cut(s) | 571, 691, 867, 1051 |
| BtsIMutI | CAGTG | 1 cut(s) | 1672 |
| Cac8I | GCNNGC | 4 cut(s) | 310, 953, 1006, 1342 |
| CciI | TCATGA | 2 cut(s) | 1536, 1791 |
| CfoI | GCGC | 3 cut(s) | 1198, 1302, 1788 |
| Cfr13I | GGNCC | 3 cut(s) | 342, 682, 683 |
| Csp6I | GTAC | 2 cut(s) | 241, 1387 |
| CviAII | CATG | 9 cut(s) | 391, 673, 952, 1151, 1345, 1426, 1537, 1792, 1807 |
| CviQI | GTAC | 2 cut(s) | 241, 1387 |
| DdeI | CTNAG | 9 cut(s) | 21, 123, 154, 224, 837, 894, 911, 1248, 1735 |
| DinI | GGCGCC | 1 cut(s) | 1197 |
| DpnI | GATC | 7 cut(s) | 320, 945, 1030, 1486, 1568, 1614, 1812 |
| DpnII | GATC | 7 cut(s) | 318, 943, 1028, 1484, 1566, 1612, 1810 |
| DraI | TTTAAA | 2 cut(s) | 1209, 1674 |
| EaeI | YGGCCR | 1 cut(s) | 265 |
| Eam1104I | CTCTTC | 6 cut(s) | 200, 810, 843, 1077, 1104, 1638 |
| EarI | CTCTTC | 6 cut(s) | 200, 810, 843, 1077, 1104, 1638 |
| Eco130I | CCWWGG | 6 cut(s) | 354, 409, 672, 731, 1818, 1842 |
| Eco147I | AGGCCT | 1 cut(s) | 854 |
| Eco24I | GRGCYC | 1 cut(s) | 686 |
| Eco31I | GGTCTC | 1 cut(s) | 1589 |
| Eco47I | GGWCC | 1 cut(s) | 342 |
| Eco57I | CTGAAG | 2 cut(s) | 1341, 1493 |
| Eco81I | CCTNAGG | 1 cut(s) | 1248 |
| Eco88I | CYCGRG | 2 cut(s) | 686, 1698 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 1247, 1620 |
| EcoO109I | RGGNCCY | 1 cut(s) | 683 |
| EcoRII | CCWGG | 5 cut(s) | 314, 694, 1552, 1621, 1851 |
| EcoT14I | CCWWGG | 6 cut(s) | 354, 409, 672, 731, 1818, 1842 |
| EcoT38I | GRGCYC | 1 cut(s) | 686 |
| EgeI | GGCGCC | 1 cut(s) | 1197 |
| EheI | GGCGCC | 1 cut(s) | 1197 |
| ErhI | CCWWGG | 6 cut(s) | 354, 409, 672, 731, 1818, 1842 |
| FaeI | CATG | 9 cut(s) | 394, 676, 955, 1154, 1348, 1429, 1540, 1795, 1810 |
| FaqI | GGGAC | 2 cut(s) | 328, 1811 |
| FatI | CATG | 9 cut(s) | 390, 672, 951, 1150, 1344, 1425, 1536, 1791, 1806 |
| FauI | CCCGC | 1 cut(s) | 1763 |
| FblI | GTMKAC | 2 cut(s) | 467, 1638 |
| Fnu4HI | GCNGC | 5 cut(s) | 655, 1009, 1423, 1500, 1558 |
| FokI | GGATG | 4 cut(s) | 578, 678, 874, 1058 |
| FriOI | GRGCYC | 1 cut(s) | 686 |
| Fsp4HI | GCNGC | 5 cut(s) | 655, 1009, 1423, 1500, 1558 |
| FspBI | CTAG | 2 cut(s) | 870, 1005 |
| GlaI | GCGC | 3 cut(s) | 1197, 1301, 1787 |
| GluI | GCNGC | 5 cut(s) | 655, 1009, 1423, 1500, 1558 |
| GsaI | CCCAGC | 1 cut(s) | 1758 |
| GsuI | CTGGAG | 2 cut(s) | 298, 1035 |
| HaeII | RGCGCY | 1 cut(s) | 1199 |
| HaeIII | GGCC | 6 cut(s) | 229, 267, 408, 684, 854, 1557 |
| HapII | CCGG | 1 cut(s) | 1891 |
| HhaI | GCGC | 3 cut(s) | 1198, 1302, 1788 |
| Hin1I | GRCGYC | 1 cut(s) | 1196 |
| Hin1II | CATG | 9 cut(s) | 394, 676, 955, 1154, 1348, 1429, 1540, 1795, 1810 |
| Hin6I | GCGC | 3 cut(s) | 1196, 1300, 1786 |
| HinP1I | GCGC | 3 cut(s) | 1196, 1300, 1786 |
| HincII | GTYRAC | 2 cut(s) | 388, 1639 |
| HindII | GTYRAC | 2 cut(s) | 388, 1639 |
| HindIII | AAGCTT | 3 cut(s) | 41, 212, 1857 |
| HinfI | GANTC | 4 cut(s) | 26, 161, 602, 913 |
| HpaII | CCGG | 1 cut(s) | 1891 |
| HphI | GGTGA | 4 cut(s) | 778, 1031, 1307, 1535 |
| Hpy166II | GTNNAC | 6 cut(s) | 331, 388, 468, 1526, 1639, 1663 |
| Hpy188I | TCNGA | 6 cut(s) | 157, 607, 631, 644, 997, 1512 |
| Hpy8I | GTNNAC | 6 cut(s) | 331, 388, 468, 1526, 1639, 1663 |
| Hpy99I | CGWCG | 1 cut(s) | 1643 |
| HpyCH4III | ACNGT | 7 cut(s) | 782, 794, 1366, 1453, 1523, 1600, 1667 |
| HpyCH4IV | ACGT | 1 cut(s) | 1708 |
| HpyCH4V | TGCA | 4 cut(s) | 286, 616, 1340, 1422 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 414, 575, 1431 |
| HpyF3I | CTNAG | 9 cut(s) | 21, 123, 154, 224, 837, 894, 911, 1248, 1735 |
| HpySE526I | ACGT | 1 cut(s) | 1708 |
| Hsp92I | GRCGYC | 1 cut(s) | 1196 |
| Hsp92II | CATG | 9 cut(s) | 394, 676, 955, 1154, 1348, 1429, 1540, 1795, 1810 |
| HspAI | GCGC | 3 cut(s) | 1196, 1300, 1786 |
| KasI | GGCGCC | 1 cut(s) | 1195 |
| Kpn2I | TCCGGA | 1 cut(s) | 1890 |
| Kzo9I | GATC | 7 cut(s) | 318, 943, 1028, 1484, 1566, 1612, 1810 |
| LmnI | GCTCC | 6 cut(s) | 38, 61, 317, 367, 901, 1358 |
| Lsp1109I | GCAGC | 4 cut(s) | 666, 995, 1434, 1511 |
| LweI | GCATC | 5 cut(s) | 273, 556, 700, 1530, 1773 |
| MaeI | CTAG | 2 cut(s) | 870, 1005 |
| MaeII | ACGT | 1 cut(s) | 1708 |
| MalI | GATC | 7 cut(s) | 320, 945, 1030, 1486, 1568, 1614, 1812 |
| MboI | GATC | 7 cut(s) | 318, 943, 1028, 1484, 1566, 1612, 1810 |
| MfeI | CAATTG | 3 cut(s) | 231, 881, 1272 |
| MflI | RGATCY | 5 cut(s) | 318, 1028, 1566, 1612, 1810 |
| MhlI | GDGCHC | 2 cut(s) | 188, 686 |
| MlsI | TGGCCA | 1 cut(s) | 267 |
| MluNI | TGGCCA | 1 cut(s) | 267 |
| Mly113I | GGCGCC | 1 cut(s) | 1196 |
| MlyI | GAGTC | 2 cut(s) | 611, 922 |
| Mox20I | TGGCCA | 1 cut(s) | 267 |
| MroI | TCCGGA | 1 cut(s) | 1890 |
| MroXI | GAANNNNTTC | 1 cut(s) | 458 |
| MscI | TGGCCA | 1 cut(s) | 267 |
| MseI | TTAA | 8 cut(s) | 518, 797, 857, 929, 957, 1208, 1673, 1926 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1805 |
| Msp20I | TGGCCA | 1 cut(s) | 267 |
| MspCI | CTTAAG | 1 cut(s) | 856 |
| MspI | CCGG | 1 cut(s) | 1891 |
| MspR9I | CCNGG | 5 cut(s) | 316, 696, 1554, 1623, 1853 |
| MunI | CAATTG | 3 cut(s) | 231, 881, 1272 |
| Mva1269I | GAATGC | 1 cut(s) | 1772 |
| MvaI | CCWGG | 5 cut(s) | 316, 696, 1554, 1623, 1853 |
| MvnI | CGCG | 1 cut(s) | 1302 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 414, 575, 1431 |
| NarI | GGCGCC | 1 cut(s) | 1196 |
| NcoI | CCATGG | 1 cut(s) | 672 |
| NdeII | GATC | 7 cut(s) | 318, 943, 1028, 1484, 1566, 1612, 1810 |
| NheI | GCTAGC | 1 cut(s) | 1004 |
| NlaIII | CATG | 9 cut(s) | 394, 676, 955, 1154, 1348, 1429, 1540, 1795, 1810 |
| NlaIV | GGNNCC | 8 cut(s) | 34, 320, 344, 684, 685, 1197, 1614, 1812 |
| NspI | RCATGY | 1 cut(s) | 955 |
| NspV | TTCGAA | 2 cut(s) | 74, 92 |
| PaeI | GCATGC | 1 cut(s) | 955 |
| PagI | TCATGA | 2 cut(s) | 1536, 1791 |
| PceI | AGGCCT | 1 cut(s) | 854 |
| PctI | GAATGC | 1 cut(s) | 1772 |
| PdmI | GAANNNNTTC | 1 cut(s) | 458 |
| PfeI | GAWTC | 2 cut(s) | 26, 161 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 450 |
| PfoI | TCCNGGA | 1 cut(s) | 314 |
| PkrI | GCNGC | 5 cut(s) | 656, 1010, 1424, 1501, 1559 |
| PleI | GAGTC | 2 cut(s) | 610, 921 |
| PluTI | GGCGCC | 1 cut(s) | 1199 |
| PpsI | GAGTC | 2 cut(s) | 610, 921 |
| PshBI | ATTAAT | 1 cut(s) | 929 |
| Psp6I | CCWGG | 5 cut(s) | 314, 694, 1552, 1621, 1851 |
| PspFI | CCCAGC | 1 cut(s) | 1754 |
| PspGI | CCWGG | 5 cut(s) | 314, 694, 1552, 1621, 1851 |
| PspN4I | GGNNCC | 8 cut(s) | 34, 320, 344, 684, 685, 1197, 1614, 1812 |
| PspOMI | GGGCCC | 1 cut(s) | 682 |
| PspPI | GGNCC | 3 cut(s) | 342, 682, 683 |
| PsuI | RGATCY | 5 cut(s) | 318, 1028, 1566, 1612, 1810 |
| RsaI | GTAC | 2 cut(s) | 242, 1388 |
| RsaNI | GTAC | 2 cut(s) | 241, 1387 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1805 |
| SalI | GTCGAC | 1 cut(s) | 1637 |
| SaqAI | TTAA | 8 cut(s) | 518, 797, 857, 929, 957, 1208, 1673, 1926 |
| SatI | GCNGC | 5 cut(s) | 655, 1009, 1423, 1500, 1558 |
| Sau3AI | GATC | 7 cut(s) | 318, 943, 1028, 1484, 1566, 1612, 1810 |
| Sau96I | GGNCC | 3 cut(s) | 342, 682, 683 |
| SchI | GAGTC | 2 cut(s) | 611, 922 |
| ScrFI | CCNGG | 5 cut(s) | 316, 696, 1554, 1623, 1853 |
| SduI | GDGCHC | 2 cut(s) | 188, 686 |
| SfaNI | GCATC | 5 cut(s) | 273, 556, 700, 1530, 1773 |
| SfcI | CTRYAG | 1 cut(s) | 1449 |
| SfoI | GGCGCC | 1 cut(s) | 1197 |
| SfuI | TTCGAA | 2 cut(s) | 74, 92 |
| SinI | GGWCC | 1 cut(s) | 342 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1805 |
| SmlI | CTYRAG | 2 cut(s) | 60, 856 |
| SmoI | CTYRAG | 2 cut(s) | 60, 856 |
| SphI | GCATGC | 1 cut(s) | 955 |
| SseBI | AGGCCT | 1 cut(s) | 854 |
| SsiI | CCGC | 2 cut(s) | 1558, 1770 |
| SspDI | GGCGCC | 1 cut(s) | 1195 |
| SspI | AATATT | 1 cut(s) | 740 |
| SspMI | CTAG | 2 cut(s) | 870, 1005 |
| StuI | AGGCCT | 1 cut(s) | 854 |
| StyD4I | CCNGG | 5 cut(s) | 314, 694, 1552, 1621, 1851 |
| StyI | CCWWGG | 6 cut(s) | 354, 409, 672, 731, 1818, 1842 |
| TaaI | ACNGT | 7 cut(s) | 782, 794, 1366, 1453, 1523, 1600, 1667 |
| TaiI | ACGT | 1 cut(s) | 1711 |
| TaqI | TCGA | 6 cut(s) | 74, 92, 175, 1504, 1638, 1764 |
| TatI | WGTACW | 1 cut(s) | 240 |
| TauI | GCSGC | 1 cut(s) | 1560 |
| TfiI | GAWTC | 2 cut(s) | 26, 161 |
| Tru1I | TTAA | 8 cut(s) | 518, 797, 857, 929, 957, 1208, 1673, 1926 |
| Tru9I | TTAA | 8 cut(s) | 518, 797, 857, 929, 957, 1208, 1673, 1926 |
| TscAI | CASTG | 1 cut(s) | 1672 |
| TseI | GCWGC | 4 cut(s) | 654, 1008, 1422, 1499 |
| TspRI | CASTG | 1 cut(s) | 1672 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 450 |
| Vha464I | CTTAAG | 1 cut(s) | 856 |
| VpaK11BI | GGWCC | 1 cut(s) | 342 |
| VspI | ATTAAT | 1 cut(s) | 929 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 1247, 1620 |
| XapI | RAATTY | 1 cut(s) | 542 |
| XceI | RCATGY | 1 cut(s) | 955 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1560 |
| XmiI | GTMKAC | 2 cut(s) | 467, 1638 |
| XmnI | GAANNNNTTC | 1 cut(s) | 458 |
| XspI | CTAG | 2 cut(s) | 870, 1005 |