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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AatII | GACGTC | 1 cut(s) | 1446 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1308 |
| AccIII | TCCGGA | 1 cut(s) | 204 |
| AciI | CCGC | 5 cut(s) | 99, 123, 182, 1063, 1070 |
| AclWI | GGATC | 5 cut(s) | 224, 891, 997, 1319, 1427 |
| AcoI | YGGCCR | 1 cut(s) | 907 |
| AcsI | RAATTY | 4 cut(s) | 46, 485, 916, 1209 |
| AcyI | GRCGYC | 1 cut(s) | 1443 |
| AfaI | GTAC | 3 cut(s) | 408, 655, 1144 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 191, 1184, 1308, 1563 |
| AjnI | CCWGG | 1 cut(s) | 781 |
| AluBI | AGCT | 7 cut(s) | 413, 509, 699, 727, 802, 870, 1171 |
| AluI | AGCT | 7 cut(s) | 413, 509, 699, 727, 802, 870, 1171 |
| Alw21I | GWGCWC | 1 cut(s) | 325 |
| Alw26I | GTCTC | 5 cut(s) | 1222, 1349, 1391, 1434, 1454 |
| AlwI | GGATC | 5 cut(s) | 224, 891, 997, 1319, 1427 |
| Ama87I | CYCGRG | 3 cut(s) | 752, 1040, 1414 |
| Aor13HI | TCCGGA | 1 cut(s) | 204 |
| AoxI | GGCC | 4 cut(s) | 176, 907, 1299, 1452 |
| ApeKI | GCWGC | 3 cut(s) | 53, 1171, 1535 |
| ApoI | RAATTY | 4 cut(s) | 46, 485, 916, 1209 |
| AseI | ATTAAT | 1 cut(s) | 255 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 396 |
| AspLEI | GCGC | 1 cut(s) | 369 |
| AspS9I | GGNCC | 2 cut(s) | 379, 1299 |
| AsuC2I | CCSGG | 3 cut(s) | 58, 1415, 1416 |
| AsuHPI | GGTGA | 5 cut(s) | 137, 509, 1029, 1084, 1292 |
| AsuII | TTCGAA | 2 cut(s) | 614, 998 |
| AsuNHI | GCTAGC | 2 cut(s) | 509, 769 |
| AvaI | CYCGRG | 3 cut(s) | 752, 1040, 1414 |
| AvaII | GGWCC | 1 cut(s) | 379 |
| AxyI | CCTNAGG | 1 cut(s) | 598 |
| BalI | TGGCCA | 1 cut(s) | 909 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 425, 457 |
| BbsI | GAAGAC | 3 cut(s) | 440, 944, 1124 |
| Bbv12I | GWGCWC | 1 cut(s) | 325 |
| BbvI | GCAGC | 3 cut(s) | 40, 1158, 1547 |
| BccI | CCATC | 6 cut(s) | 207, 310, 563, 759, 1328, 1483 |
| BceAI | ACGGC | 1 cut(s) | 678 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 199, 233 |
| BciT130I | CCWGG | 1 cut(s) | 783 |
| BciVI | GTATCC | 1 cut(s) | 863 |
| BcnI | CCSGG | 3 cut(s) | 58, 1415, 1416 |
| BcoDI | GTCTC | 5 cut(s) | 1222, 1349, 1391, 1434, 1454 |
| BfaI | CTAG | 7 cut(s) | 6, 191, 410, 506, 510, 690, 770 |
| BfmI | CTRYAG | 1 cut(s) | 840 |
| BfuI | GTATCC | 1 cut(s) | 863 |
| BisI | GCNGC | 4 cut(s) | 54, 1064, 1172, 1536 |
| BlsI | GCNGC | 4 cut(s) | 55, 1065, 1173, 1537 |
| BmcAI | AGTACT | 1 cut(s) | 655 |
| Bme1390I | CCNGG | 4 cut(s) | 58, 783, 1415, 1416 |
| Bme18I | GGWCC | 1 cut(s) | 379 |
| BmeT110I | CYCGRG | 3 cut(s) | 752, 1040, 1414 |
| BmgT120I | GGNCC | 2 cut(s) | 379, 1299 |
| BmiI | GGNNCC | 1 cut(s) | 380 |
| BmrFI | CCNGG | 4 cut(s) | 58, 783, 1415, 1416 |
| BmsI | GCATC | 1 cut(s) | 1513 |
| BmtI | GCTAGC | 2 cut(s) | 513, 773 |
| BoxI | GACNNNNGTC | 2 cut(s) | 501, 1403 |
| BpiI | GAAGAC | 3 cut(s) | 440, 944, 1124 |
| Bpu10I | CCTNAGC | 1 cut(s) | 1164 |
| Bpu14I | TTCGAA | 2 cut(s) | 614, 998 |
| BpuMI | CCSGG | 3 cut(s) | 58, 1415, 1416 |
| Bsa29I | ATCGAT | 1 cut(s) | 459 |
| BsaBI | GATNNNNATC | 1 cut(s) | 882 |
| BsaHI | GRCGYC | 1 cut(s) | 1443 |
| BsaI | GGTCTC | 2 cut(s) | 1391, 1454 |
| BsaJI | CCNNGG | 7 cut(s) | 57, 782, 1329, 1414, 1415, 1449, 1455 |
| BsaWI | WCCGGW | 1 cut(s) | 204 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 191, 1184, 1308, 1563 |
| Bse1I | ACTGG | 2 cut(s) | 856, 1552 |
| Bse21I | CCTNAGG | 1 cut(s) | 598 |
| Bse8I | GATNNNNATC | 1 cut(s) | 882 |
| BseAI | TCCGGA | 1 cut(s) | 204 |
| BseBI | CCWGG | 1 cut(s) | 783 |
| BseCI | ATCGAT | 1 cut(s) | 459 |
| BseDI | CCNNGG | 7 cut(s) | 57, 782, 1329, 1414, 1415, 1449, 1455 |
| BseGI | GGATG | 6 cut(s) | 747, 1260, 1339, 1354, 1475, 1528 |
| BseJI | GATNNNNATC | 1 cut(s) | 882 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 191, 1184, 1308, 1563 |
| BseNI | ACTGG | 2 cut(s) | 856, 1552 |
| BseXI | GCAGC | 3 cut(s) | 40, 1158, 1547 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1435 |
| BshFI | GGCC | 4 cut(s) | 178, 909, 1301, 1454 |
| BshVI | ATCGAT | 1 cut(s) | 459 |
| BsiEI | CGRYCG | 1 cut(s) | 1435 |
| BsiHKAI | GWGCWC | 1 cut(s) | 325 |
| BsiHKCI | CYCGRG | 3 cut(s) | 752, 1040, 1414 |
| BsiSI | CCGG | 4 cut(s) | 57, 67, 205, 1415 |
| BslFI | GGGAC | 3 cut(s) | 392, 821, 1397 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 191, 1184, 1308, 1563 |
| BsmAI | GTCTC | 5 cut(s) | 1222, 1349, 1391, 1434, 1454 |
| BsmBI | CGTCTC | 1 cut(s) | 1434 |
| BsmFI | GGGAC | 3 cut(s) | 392, 821, 1397 |
| BsmI | GAATGC | 2 cut(s) | 513, 1513 |
| BsnI | GGCC | 4 cut(s) | 178, 909, 1301, 1454 |
| Bso31I | GGTCTC | 2 cut(s) | 1391, 1454 |
| BsoBI | CYCGRG | 3 cut(s) | 752, 1040, 1414 |
| Bsp119I | TTCGAA | 2 cut(s) | 614, 998 |
| Bsp1286I | GDGCHC | 1 cut(s) | 325 |
| Bsp13I | TCCGGA | 1 cut(s) | 204 |
| Bsp143I | GATC | 7 cut(s) | 216, 883, 989, 1275, 1311, 1419, 1432 |
| Bsp19I | CCATGG | 1 cut(s) | 1455 |
| BspACI | CCGC | 5 cut(s) | 99, 123, 182, 1063, 1070 |
| BspANI | GGCC | 4 cut(s) | 178, 909, 1301, 1454 |
| BspDI | ATCGAT | 1 cut(s) | 459 |
| BspEI | TCCGGA | 1 cut(s) | 204 |
| BspHI | TCATGA | 1 cut(s) | 607 |
| BspLI | GGNNCC | 1 cut(s) | 380 |
| BspOI | GCTAGC | 2 cut(s) | 513, 773 |
| BspPI | GGATC | 5 cut(s) | 224, 891, 997, 1319, 1427 |
| BspT104I | TTCGAA | 2 cut(s) | 614, 998 |
| BspTNI | GGTCTC | 2 cut(s) | 1391, 1454 |
| BsrI | ACTGG | 2 cut(s) | 856, 1552 |
| BssECI | CCNNGG | 7 cut(s) | 57, 782, 1329, 1414, 1415, 1449, 1455 |
| BssMI | GATC | 7 cut(s) | 216, 883, 989, 1275, 1311, 1419, 1432 |
| BssNI | GRCGYC | 1 cut(s) | 1443 |
| BssT1I | CCWWGG | 2 cut(s) | 1329, 1455 |
| Bst2UI | CCWGG | 1 cut(s) | 783 |
| Bst4CI | ACNGT | 6 cut(s) | 551, 653, 844, 1123, 1411, 1519 |
| Bst6I | CTCTTC | 1 cut(s) | 1347 |
| BstACI | GRCGYC | 1 cut(s) | 1443 |
| BstBI | TTCGAA | 2 cut(s) | 614, 998 |
| BstC8I | GCNNGC | 6 cut(s) | 511, 701, 771, 897, 1068, 1540 |
| BstDEI | CTNAG | 4 cut(s) | 414, 598, 942, 1164 |
| BstDSI | CCRYGG | 1 cut(s) | 1455 |
| BstF5I | GGATG | 6 cut(s) | 747, 1260, 1339, 1354, 1475, 1528 |
| BstHHI | GCGC | 1 cut(s) | 369 |
| BstKTI | GATC | 7 cut(s) | 219, 886, 992, 1278, 1314, 1422, 1435 |
| BstMAI | GTCTC | 5 cut(s) | 1222, 1349, 1391, 1434, 1454 |
| BstMBI | GATC | 7 cut(s) | 216, 883, 989, 1275, 1311, 1419, 1432 |
| BstMCI | CGRYCG | 1 cut(s) | 1435 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 929, 1532 |
| BstNI | CCWGG | 1 cut(s) | 783 |
| BstNSI | RCATGY | 1 cut(s) | 1070 |
| BstPAI | GACNNNNGTC | 2 cut(s) | 501, 1403 |
| BstSCI | CCNGG | 4 cut(s) | 56, 781, 1413, 1414 |
| BstSFI | CTRYAG | 1 cut(s) | 840 |
| BstV1I | GCAGC | 3 cut(s) | 40, 1158, 1547 |
| BstV2I | GAAGAC | 3 cut(s) | 440, 944, 1124 |
| BstX2I | RGATCY | 1 cut(s) | 989 |
| BstYI | RGATCY | 1 cut(s) | 989 |
| Bsu15I | ATCGAT | 1 cut(s) | 459 |
| Bsu36I | CCTNAGG | 1 cut(s) | 598 |
| BsuI | GTATCC | 1 cut(s) | 863 |
| BsuRI | GGCC | 4 cut(s) | 178, 909, 1301, 1454 |
| BsuTUI | ATCGAT | 1 cut(s) | 459 |
| BtgI | CCRYGG | 1 cut(s) | 1455 |
| BtsCI | GGATG | 6 cut(s) | 747, 1260, 1339, 1354, 1475, 1528 |
| BtsIMutI | CAGTG | 2 cut(s) | 849, 1524 |
| Cac8I | GCNNGC | 6 cut(s) | 511, 701, 771, 897, 1068, 1540 |
| CciI | TCATGA | 1 cut(s) | 607 |
| CfoI | GCGC | 1 cut(s) | 369 |
| Cfr13I | GGNCC | 2 cut(s) | 379, 1299 |
| Cfr9I | CCCGGG | 1 cut(s) | 1414 |
| ClaI | ATCGAT | 1 cut(s) | 459 |
| Csp6I | GTAC | 3 cut(s) | 407, 654, 1143 |
| CspCI | CAANNNNNGTGG | 4 cut(s) | 331, 366, 1500, 1535 |
| CviAII | CATG | 9 cut(s) | 364, 608, 713, 849, 887, 1067, 1083, 1387, 1456 |
| CviQI | GTAC | 3 cut(s) | 407, 654, 1143 |
| DdeI | CTNAG | 4 cut(s) | 414, 598, 942, 1164 |
| DpnI | GATC | 7 cut(s) | 218, 885, 991, 1277, 1313, 1421, 1434 |
| DpnII | GATC | 7 cut(s) | 216, 883, 989, 1275, 1311, 1419, 1432 |
| EaeI | YGGCCR | 1 cut(s) | 907 |
| Eam1104I | CTCTTC | 1 cut(s) | 1347 |
| EarI | CTCTTC | 1 cut(s) | 1347 |
| Eco130I | CCWWGG | 2 cut(s) | 1329, 1455 |
| Eco31I | GGTCTC | 2 cut(s) | 1391, 1454 |
| Eco47I | GGWCC | 1 cut(s) | 379 |
| Eco81I | CCTNAGG | 1 cut(s) | 598 |
| Eco88I | CYCGRG | 3 cut(s) | 752, 1040, 1414 |
| EcoO109I | RGGNCCY | 1 cut(s) | 379 |
| EcoRI | GAATTC | 2 cut(s) | 485, 1209 |
| EcoRII | CCWGG | 1 cut(s) | 781 |
| EcoT14I | CCWWGG | 2 cut(s) | 1329, 1455 |
| EcoT22I | ATGCAT | 1 cut(s) | 1088 |
| ErhI | CCWWGG | 2 cut(s) | 1329, 1455 |
| Esp3I | CGTCTC | 1 cut(s) | 1434 |
| FaeI | CATG | 9 cut(s) | 367, 611, 716, 852, 890, 1070, 1086, 1390, 1459 |
| FaqI | GGGAC | 3 cut(s) | 392, 821, 1397 |
| FatI | CATG | 9 cut(s) | 363, 607, 712, 848, 886, 1066, 1082, 1386, 1455 |
| Fnu4HI | GCNGC | 4 cut(s) | 54, 1064, 1172, 1536 |
| FokI | GGATG | 6 cut(s) | 734, 1267, 1346, 1361, 1462, 1535 |
| Fsp4HI | GCNGC | 4 cut(s) | 54, 1064, 1172, 1536 |
| FspBI | CTAG | 7 cut(s) | 6, 191, 410, 506, 510, 690, 770 |
| GlaI | GCGC | 1 cut(s) | 368 |
| GluI | GCNGC | 4 cut(s) | 54, 1064, 1172, 1536 |
| HaeIII | GGCC | 4 cut(s) | 178, 909, 1301, 1454 |
| HapII | CCGG | 4 cut(s) | 57, 67, 205, 1415 |
| HhaI | GCGC | 1 cut(s) | 369 |
| Hin1I | GRCGYC | 1 cut(s) | 1443 |
| Hin1II | CATG | 9 cut(s) | 367, 611, 716, 852, 890, 1070, 1086, 1390, 1459 |
| Hin6I | GCGC | 1 cut(s) | 367 |
| HinP1I | GCGC | 1 cut(s) | 367 |
| HinfI | GANTC | 5 cut(s) | 611, 622, 1034, 1045, 1102 |
| HpaII | CCGG | 4 cut(s) | 57, 67, 205, 1415 |
| HphI | GGTGA | 5 cut(s) | 137, 509, 1029, 1084, 1292 |
| Hpy166II | GTNNAC | 1 cut(s) | 407 |
| Hpy188I | TCNGA | 1 cut(s) | 221 |
| Hpy188III | TCNNGA | 7 cut(s) | 205, 313, 449, 608, 983, 1252, 1430 |
| Hpy8I | GTNNAC | 1 cut(s) | 407 |
| Hpy99I | CGWCG | 2 cut(s) | 989, 1448 |
| HpyAV | CCTTC | 8 cut(s) | 416, 455, 693, 943, 1198, 1314, 1360, 1481 |
| HpyCH4III | ACNGT | 6 cut(s) | 551, 653, 844, 1123, 1411, 1519 |
| HpyCH4IV | ACGT | 4 cut(s) | 289, 360, 1404, 1443 |
| HpyCH4V | TGCA | 6 cut(s) | 251, 895, 923, 1086, 1174, 1511 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 929, 1532 |
| HpyF3I | CTNAG | 4 cut(s) | 414, 598, 942, 1164 |
| HpySE526I | ACGT | 4 cut(s) | 289, 360, 1404, 1443 |
| Hsp92I | GRCGYC | 1 cut(s) | 1443 |
| Hsp92II | CATG | 9 cut(s) | 367, 611, 716, 852, 890, 1070, 1086, 1390, 1459 |
| HspAI | GCGC | 1 cut(s) | 367 |
| Kpn2I | TCCGGA | 1 cut(s) | 204 |
| Kzo9I | GATC | 7 cut(s) | 216, 883, 989, 1275, 1311, 1419, 1432 |
| LmnI | GCTCC | 3 cut(s) | 207, 328, 1168 |
| Lsp1109I | GCAGC | 3 cut(s) | 40, 1158, 1547 |
| LweI | GCATC | 1 cut(s) | 1513 |
| MaeI | CTAG | 7 cut(s) | 6, 191, 410, 506, 510, 690, 770 |
| MaeII | ACGT | 4 cut(s) | 289, 360, 1404, 1443 |
| MaeIII | GTNAC | 6 cut(s) | 125, 715, 743, 858, 1405, 1427 |
| MalI | GATC | 7 cut(s) | 218, 885, 991, 1277, 1313, 1421, 1434 |
| MboI | GATC | 7 cut(s) | 216, 883, 989, 1275, 1311, 1419, 1432 |
| MflI | RGATCY | 1 cut(s) | 989 |
| MhlI | GDGCHC | 1 cut(s) | 325 |
| MlsI | TGGCCA | 1 cut(s) | 909 |
| MluCI | AATT | 8 cut(s) | 37, 46, 170, 252, 485, 916, 960, 1209 |
| MluNI | TGGCCA | 1 cut(s) | 909 |
| MlyI | GAGTC | 2 cut(s) | 1054, 1111 |
| MmeI | TCCRAC | 2 cut(s) | 224, 860 |
| MnlI | CCTC | 8 cut(s) | 63, 134, 168, 215, 369, 874, 1444, 1492 |
| Mox20I | TGGCCA | 1 cut(s) | 909 |
| Mph1103I | ATGCAT | 1 cut(s) | 1088 |
| MroI | TCCGGA | 1 cut(s) | 204 |
| MroXI | GAANNNNTTC | 1 cut(s) | 396 |
| MscI | TGGCCA | 1 cut(s) | 909 |
| MseI | TTAA | 7 cut(s) | 15, 42, 255, 729, 1050, 1131, 1574 |
| MslI | CAYNNNNRTG | 1 cut(s) | 711 |
| Msp20I | TGGCCA | 1 cut(s) | 909 |
| MspI | CCGG | 4 cut(s) | 57, 67, 205, 1415 |
| MspR9I | CCNGG | 4 cut(s) | 58, 783, 1415, 1416 |
| Mva1269I | GAATGC | 2 cut(s) | 513, 1513 |
| MvaI | CCWGG | 1 cut(s) | 783 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 929, 1532 |
| NciI | CCSGG | 3 cut(s) | 58, 1415, 1416 |
| NcoI | CCATGG | 1 cut(s) | 1455 |
| NdeII | GATC | 7 cut(s) | 216, 883, 989, 1275, 1311, 1419, 1432 |
| NheI | GCTAGC | 2 cut(s) | 509, 769 |
| NlaIII | CATG | 9 cut(s) | 367, 611, 716, 852, 890, 1070, 1086, 1390, 1459 |
| NlaIV | GGNNCC | 1 cut(s) | 380 |
| NmeAIII | GCCGAG | 1 cut(s) | 1474 |
| NmuCI | GTSAC | 5 cut(s) | 125, 743, 858, 1405, 1427 |
| NsiI | ATGCAT | 1 cut(s) | 1088 |
| NspI | RCATGY | 1 cut(s) | 1070 |
| NspV | TTCGAA | 2 cut(s) | 614, 998 |
| PaeI | GCATGC | 1 cut(s) | 1070 |
| PagI | TCATGA | 1 cut(s) | 607 |
| PctI | GAATGC | 2 cut(s) | 513, 1513 |
| PdmI | GAANNNNTTC | 1 cut(s) | 396 |
| PfeI | GAWTC | 3 cut(s) | 611, 622, 1034 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1308 |
| PkrI | GCNGC | 4 cut(s) | 55, 1065, 1173, 1537 |
| Ple19I | CGATCG | 1 cut(s) | 1435 |
| PleI | GAGTC | 2 cut(s) | 1053, 1110 |
| PpsI | GAGTC | 2 cut(s) | 1053, 1110 |
| PpuMI | RGGWCCY | 1 cut(s) | 379 |
| PshAI | GACNNNNGTC | 2 cut(s) | 501, 1403 |
| PshBI | ATTAAT | 1 cut(s) | 255 |
| Psp5II | RGGWCCY | 1 cut(s) | 379 |
| Psp6I | CCWGG | 1 cut(s) | 781 |
| PspGI | CCWGG | 1 cut(s) | 781 |
| PspN4I | GGNNCC | 1 cut(s) | 380 |
| PspPI | GGNCC | 2 cut(s) | 379, 1299 |
| PspPPI | RGGWCCY | 1 cut(s) | 379 |
| PsuI | RGATCY | 1 cut(s) | 989 |
| PvuI | CGATCG | 1 cut(s) | 1435 |
| RsaI | GTAC | 3 cut(s) | 408, 655, 1144 |
| RsaNI | GTAC | 3 cut(s) | 407, 654, 1143 |
| RseI | CAYNNNNRTG | 1 cut(s) | 711 |
| SaqAI | TTAA | 7 cut(s) | 15, 42, 255, 729, 1050, 1131, 1574 |
| SatI | GCNGC | 4 cut(s) | 54, 1064, 1172, 1536 |
| Sau3AI | GATC | 7 cut(s) | 216, 883, 989, 1275, 1311, 1419, 1432 |
| Sau96I | GGNCC | 2 cut(s) | 379, 1299 |
| ScaI | AGTACT | 1 cut(s) | 655 |
| SchI | GAGTC | 2 cut(s) | 1054, 1111 |
| ScrFI | CCNGG | 4 cut(s) | 58, 783, 1415, 1416 |
| SduI | GDGCHC | 1 cut(s) | 325 |
| SfaNI | GCATC | 1 cut(s) | 1513 |
| SfcI | CTRYAG | 1 cut(s) | 840 |
| SfuI | TTCGAA | 2 cut(s) | 614, 998 |
| SinI | GGWCC | 1 cut(s) | 379 |
| SmaI | CCCGGG | 1 cut(s) | 1416 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 711 |
| SphI | GCATGC | 1 cut(s) | 1070 |
| Sse9I | AATT | 8 cut(s) | 37, 46, 170, 252, 485, 916, 960, 1209 |
| SsiI | CCGC | 5 cut(s) | 99, 123, 182, 1063, 1070 |
| SspMI | CTAG | 7 cut(s) | 6, 191, 410, 506, 510, 690, 770 |
| StyD4I | CCNGG | 4 cut(s) | 56, 781, 1413, 1414 |
| StyI | CCWWGG | 2 cut(s) | 1329, 1455 |
| TaaI | ACNGT | 6 cut(s) | 551, 653, 844, 1123, 1411, 1519 |
| TaiI | ACGT | 4 cut(s) | 292, 363, 1407, 1446 |
| TaqI | TCGA | 6 cut(s) | 74, 459, 614, 620, 984, 998 |
| TaqII | GACCGA | 1 cut(s) | 890 |
| TasI | AATT | 8 cut(s) | 37, 46, 170, 252, 485, 916, 960, 1209 |
| TatI | WGTACW | 2 cut(s) | 406, 653 |
| TauI | GCSGC | 1 cut(s) | 1066 |
| TfiI | GAWTC | 3 cut(s) | 611, 622, 1034 |
| Tru1I | TTAA | 7 cut(s) | 15, 42, 255, 729, 1050, 1131, 1574 |
| Tru9I | TTAA | 7 cut(s) | 15, 42, 255, 729, 1050, 1131, 1574 |
| TscAI | CASTG | 2 cut(s) | 849, 1524 |
| TseFI | GTSAC | 5 cut(s) | 125, 743, 858, 1405, 1427 |
| TseI | GCWGC | 3 cut(s) | 53, 1171, 1535 |
| Tsp45I | GTSAC | 5 cut(s) | 125, 743, 858, 1405, 1427 |
| TspDTI | ATGAA | 9 cut(s) | 446, 477, 560, 596, 675, 747, 804, 1299, 1365 |
| TspGWI | ACGGA | 2 cut(s) | 458, 1002 |
| TspMI | CCCGGG | 1 cut(s) | 1414 |
| TspRI | CASTG | 2 cut(s) | 849, 1524 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1308 |
| VpaK11BI | GGWCC | 1 cut(s) | 379 |
| VspI | ATTAAT | 1 cut(s) | 255 |
| XapI | RAATTY | 4 cut(s) | 46, 485, 916, 1209 |
| XceI | RCATGY | 1 cut(s) | 1070 |
| XmaI | CCCGGG | 1 cut(s) | 1414 |
| XmnI | GAANNNNTTC | 1 cut(s) | 396 |
| XspI | CTAG | 7 cut(s) | 6, 191, 410, 506, 510, 690, 770 |
| ZraI | GACGTC | 1 cut(s) | 1444 |
| ZrmI | AGTACT | 1 cut(s) | 655 |
| Zsp2I | ATGCAT | 1 cut(s) | 1088 |