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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AluBI | AGCT | 1 cut(s) | 110 |
| AluI | AGCT | 1 cut(s) | 110 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 116 |
| CviAII | CATG | 1 cut(s) | 125 |
| CviJI | RGCY | 1 cut(s) | 110 |
| CviKI_1 | RGCY | 1 cut(s) | 110 |
| FaeI | CATG | 1 cut(s) | 128 |
| FaiI | YATR | 5 cut(s) | 9, 11, 45, 126, 131 |
| FatI | CATG | 1 cut(s) | 124 |
| Hin1II | CATG | 1 cut(s) | 128 |
| HpyAV | CCTTC | 1 cut(s) | 82 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 116 |
| Hsp92II | CATG | 1 cut(s) | 128 |
| MluCI | AATT | 1 cut(s) | 18 |
| MnlI | CCTC | 1 cut(s) | 19 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 116 |
| NlaIII | CATG | 1 cut(s) | 128 |
| SetI | ASST | 1 cut(s) | 112 |
| SgeI | CNNG | 6 cut(s) | 43, 49, 64, 86, 119, 137 |
| Sse9I | AATT | 1 cut(s) | 18 |
| TaqI | TCGA | 1 cut(s) | 106 |
| TasI | AATT | 1 cut(s) | 18 |
| TspDTI | ATGAA | 1 cut(s) | 26 |