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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 44, 68 |
| AjiI | CACGTC | 1 cut(s) | 47 |
| AluBI | AGCT | 1 cut(s) | 11 |
| AluI | AGCT | 1 cut(s) | 11 |
| AlwI | GGATC | 2 cut(s) | 44, 68 |
| AoxI | GGCC | 1 cut(s) | 93 |
| BmgBI | CACGTC | 1 cut(s) | 47 |
| Bse3DI | GCAATG | 1 cut(s) | 134 |
| BseGI | GGATG | 1 cut(s) | 10 |
| BseMI | GCAATG | 1 cut(s) | 134 |
| BseRI | GAGGAG | 1 cut(s) | 101 |
| BshFI | GGCC | 1 cut(s) | 95 |
| BsnI | GGCC | 1 cut(s) | 95 |
| Bsp143I | GATC | 3 cut(s) | 19, 36, 73 |
| BspANI | GGCC | 1 cut(s) | 95 |
| BspPI | GGATC | 2 cut(s) | 44, 68 |
| BsrDI | GCAATG | 1 cut(s) | 134 |
| BssMI | GATC | 3 cut(s) | 19, 36, 73 |
| BstF5I | GGATG | 1 cut(s) | 10 |
| BstKTI | GATC | 3 cut(s) | 22, 39, 76 |
| BstMBI | GATC | 3 cut(s) | 19, 36, 73 |
| BstX2I | RGATCY | 1 cut(s) | 73 |
| BstYI | RGATCY | 1 cut(s) | 73 |
| BsuRI | GGCC | 1 cut(s) | 95 |
| BtrI | CACGTC | 1 cut(s) | 47 |
| BtsCI | GGATG | 1 cut(s) | 10 |
| CviAII | CATG | 1 cut(s) | 31 |
| CviJI | RGCY | 2 cut(s) | 11, 95 |
| CviKI_1 | RGCY | 2 cut(s) | 11, 95 |
| DpnI | GATC | 3 cut(s) | 21, 38, 75 |
| DpnII | GATC | 3 cut(s) | 19, 36, 73 |
| DraI | TTTAAA | 1 cut(s) | 70 |
| FaeI | CATG | 1 cut(s) | 34 |
| FaiI | YATR | 1 cut(s) | 32 |
| FalI | AAGNNNNNCTT | 1 cut(s) | 32 |
| FatI | CATG | 1 cut(s) | 30 |
| FokI | GGATG | 1 cut(s) | 17 |
| HaeIII | GGCC | 1 cut(s) | 95 |
| Hin1II | CATG | 1 cut(s) | 34 |
| Hpy188III | TCNNGA | 1 cut(s) | 50 |
| HpyAV | CCTTC | 1 cut(s) | 85 |
| HpyCH4IV | ACGT | 1 cut(s) | 46 |
| HpyCH4V | TGCA | 2 cut(s) | 26, 139 |
| HpySE526I | ACGT | 1 cut(s) | 46 |
| Hsp92II | CATG | 1 cut(s) | 34 |
| Kzo9I | GATC | 3 cut(s) | 19, 36, 73 |
| MaeII | ACGT | 1 cut(s) | 46 |
| MalI | GATC | 3 cut(s) | 21, 38, 75 |
| MboI | GATC | 3 cut(s) | 19, 36, 73 |
| MflI | RGATCY | 1 cut(s) | 73 |
| MluCI | AATT | 1 cut(s) | 102 |
| MnlI | CCTC | 2 cut(s) | 56, 79 |
| MseI | TTAA | 1 cut(s) | 69 |
| NdeII | GATC | 3 cut(s) | 19, 36, 73 |
| NlaIII | CATG | 1 cut(s) | 34 |
| PsuI | RGATCY | 1 cut(s) | 73 |
| SaqAI | TTAA | 1 cut(s) | 69 |
| Sau3AI | GATC | 3 cut(s) | 19, 36, 73 |
| SetI | ASST | 2 cut(s) | 13, 49 |
| SgeI | CNNG | 5 cut(s) | 29, 35, 43, 57, 62 |
| Sse9I | AATT | 1 cut(s) | 102 |
| TaiI | ACGT | 1 cut(s) | 49 |
| TasI | AATT | 1 cut(s) | 102 |
| Tru1I | TTAA | 1 cut(s) | 69 |
| Tru9I | TTAA | 1 cut(s) | 69 |
| TspDTI | ATGAA | 1 cut(s) | 21 |