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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 50 |
| AccBSI | CCGCTC | 2 cut(s) | 179, 441 |
| AccI | GTMKAC | 2 cut(s) | 236, 420 |
| AccII | CGCG | 1 cut(s) | 352 |
| AccIII | TCCGGA | 2 cut(s) | 386, 457 |
| AciI | CCGC | 7 cut(s) | 5, 177, 252, 272, 352, 439, 690 |
| AclWI | GGATC | 6 cut(s) | 138, 588, 1112, 1271, 1284, 1623 |
| AcoI | YGGCCR | 1 cut(s) | 427 |
| AcsI | RAATTY | 1 cut(s) | 1523 |
| AcuI | CTGAAG | 4 cut(s) | 867, 1121, 1338, 1422 |
| AfaI | GTAC | 3 cut(s) | 511, 1110, 1583 |
| AfeI | AGCGCT | 1 cut(s) | 160 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 231, 450, 457 |
| AflII | CTTAAG | 1 cut(s) | 595 |
| AgeI | ACCGGT | 1 cut(s) | 47 |
| AjiI | CACGTC | 1 cut(s) | 857 |
| AjnI | CCWGG | 4 cut(s) | 464, 954, 1150, 1462 |
| Alw21I | GWGCWC | 3 cut(s) | 95, 1074, 1089 |
| Alw26I | GTCTC | 3 cut(s) | 13, 210, 847 |
| AlwI | GGATC | 6 cut(s) | 138, 588, 1112, 1271, 1284, 1623 |
| Ama87I | CYCGRG | 1 cut(s) | 397 |
| Aor13HI | TCCGGA | 2 cut(s) | 386, 457 |
| Aor51HI | AGCGCT | 1 cut(s) | 160 |
| AoxI | GGCC | 3 cut(s) | 299, 427, 1212 |
| ApeKI | GCWGC | 3 cut(s) | 731, 819, 1670 |
| ApoI | RAATTY | 1 cut(s) | 1523 |
| AseI | ATTAAT | 1 cut(s) | 1302 |
| AsiGI | ACCGGT | 1 cut(s) | 47 |
| Asp700I | GAANNNNTTC | 2 cut(s) | 837, 1110 |
| AspLEI | GCGC | 2 cut(s) | 161, 1138 |
| AspS9I | GGNCC | 3 cut(s) | 171, 606, 1460 |
| AsuC2I | CCSGG | 1 cut(s) | 451 |
| AsuHPI | GGTGA | 3 cut(s) | 612, 782, 1661 |
| AsuII | TTCGAA | 1 cut(s) | 665 |
| AsuNHI | GCTAGC | 3 cut(s) | 1037, 1163, 1451 |
| AvaI | CYCGRG | 1 cut(s) | 397 |
| AvaII | GGWCC | 3 cut(s) | 171, 606, 1460 |
| BamHI | GGATCC | 1 cut(s) | 1276 |
| BanI | GGYRCC | 1 cut(s) | 50 |
| BanII | GRGCYC | 2 cut(s) | 465, 1089 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 892, 924 |
| BbsI | GAAGAC | 1 cut(s) | 681 |
| Bbv12I | GWGCWC | 3 cut(s) | 95, 1074, 1089 |
| BbvI | GCAGC | 3 cut(s) | 743, 831, 1682 |
| BccI | CCATC | 1 cut(s) | 1640 |
| BceAI | ACGGC | 1 cut(s) | 414 |
| BciT130I | CCWGG | 4 cut(s) | 466, 956, 1152, 1464 |
| BciVI | GTATCC | 1 cut(s) | 1243 |
| BcnI | CCSGG | 1 cut(s) | 451 |
| BcoDI | GTCTC | 3 cut(s) | 13, 210, 847 |
| BfaI | CTAG | 7 cut(s) | 275, 330, 564, 920, 1038, 1164, 1452 |
| BfoI | RGCGCY | 1 cut(s) | 162 |
| BfrI | CTTAAG | 1 cut(s) | 595 |
| BfuI | GTATCC | 1 cut(s) | 1243 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 469 |
| BglII | AGATCT | 1 cut(s) | 1054 |
| BisI | GCNGC | 3 cut(s) | 732, 820, 1671 |
| BlpI | GCTNAGC | 1 cut(s) | 1088 |
| BlsI | GCNGC | 3 cut(s) | 733, 821, 1672 |
| BmcAI | AGTACT | 1 cut(s) | 1110 |
| Bme1390I | CCNGG | 5 cut(s) | 451, 466, 956, 1152, 1464 |
| Bme18I | GGWCC | 3 cut(s) | 171, 606, 1460 |
| BmeT110I | CYCGRG | 1 cut(s) | 397 |
| BmgBI | CACGTC | 1 cut(s) | 857 |
| BmgT120I | GGNCC | 3 cut(s) | 171, 606, 1460 |
| BmiI | GGNNCC | 7 cut(s) | 52, 380, 407, 462, 709, 1278, 1470 |
| BmrFI | CCNGG | 5 cut(s) | 451, 466, 956, 1152, 1464 |
| BmsI | GCATC | 2 cut(s) | 1413, 1657 |
| BmtI | GCTAGC | 3 cut(s) | 1041, 1167, 1455 |
| BoxI | GACNNNNGTC | 1 cut(s) | 399 |
| BpiI | GAAGAC | 1 cut(s) | 681 |
| BpmI | CTGGAG | 1 cut(s) | 726 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1088 |
| Bpu14I | TTCGAA | 1 cut(s) | 665 |
| BpuEI | CTTGAG | 4 cut(s) | 151, 174, 1037, 1577 |
| BpuMI | CCSGG | 1 cut(s) | 451 |
| BsaI | GGTCTC | 1 cut(s) | 13 |
| BsaJI | CCNNGG | 4 cut(s) | 464, 465, 1075, 1463 |
| BsaWI | WCCGGW | 4 cut(s) | 47, 386, 457, 767 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 231, 450, 457 |
| Bse118I | RCCGGY | 2 cut(s) | 47, 301 |
| Bse1I | ACTGG | 4 cut(s) | 315, 382, 430, 609 |
| BseAI | TCCGGA | 2 cut(s) | 386, 457 |
| BseBI | CCWGG | 4 cut(s) | 466, 956, 1152, 1464 |
| BseDI | CCNNGG | 4 cut(s) | 464, 465, 1075, 1463 |
| BseGI | GGATG | 2 cut(s) | 1404, 1672 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 231, 450, 457 |
| BseMII | CTCAG | 2 cut(s) | 858, 1102 |
| BseNI | ACTGG | 4 cut(s) | 315, 382, 430, 609 |
| BseRI | GAGGAG | 1 cut(s) | 1082 |
| BseXI | GCAGC | 3 cut(s) | 743, 831, 1682 |
| BsgI | GTGCAG | 1 cut(s) | 990 |
| Bsh1236I | CGCG | 1 cut(s) | 352 |
| Bsh1285I | CGRYCG | 2 cut(s) | 344, 376 |
| BshFI | GGCC | 3 cut(s) | 301, 429, 1214 |
| BshNI | GGYRCC | 1 cut(s) | 50 |
| BshTI | ACCGGT | 1 cut(s) | 47 |
| BsiEI | CGRYCG | 2 cut(s) | 344, 376 |
| BsiHKAI | GWGCWC | 3 cut(s) | 95, 1074, 1089 |
| BsiHKCI | CYCGRG | 1 cut(s) | 397 |
| BsiSI | CCGG | 7 cut(s) | 48, 302, 387, 451, 458, 768, 914 |
| BslFI | GGGAC | 1 cut(s) | 351 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 231, 450, 457 |
| BsmAI | GTCTC | 3 cut(s) | 13, 210, 847 |
| BsmBI | CGTCTC | 1 cut(s) | 847 |
| BsmFI | GGGAC | 1 cut(s) | 351 |
| BsmI | GAATGC | 1 cut(s) | 616 |
| BsnI | GGCC | 3 cut(s) | 301, 429, 1214 |
| Bso31I | GGTCTC | 1 cut(s) | 13 |
| BsoBI | CYCGRG | 1 cut(s) | 397 |
| Bsp119I | TTCGAA | 1 cut(s) | 665 |
| Bsp1286I | GDGCHC | 4 cut(s) | 95, 465, 1074, 1089 |
| Bsp13I | TCCGGA | 2 cut(s) | 386, 457 |
| Bsp143I | GATC | 7 cut(s) | 143, 580, 842, 1054, 1117, 1276, 1628 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1088 |
| BspACI | CCGC | 7 cut(s) | 5, 177, 252, 272, 352, 439, 690 |
| BspANI | GGCC | 3 cut(s) | 301, 429, 1214 |
| BspCNI | CTCAG | 2 cut(s) | 859, 1101 |
| BspEI | TCCGGA | 2 cut(s) | 386, 457 |
| BspFNI | CGCG | 1 cut(s) | 352 |
| BspHI | TCATGA | 1 cut(s) | 1359 |
| BspLI | GGNNCC | 7 cut(s) | 52, 380, 407, 462, 709, 1278, 1470 |
| BspOI | GCTAGC | 3 cut(s) | 1041, 1167, 1455 |
| BspPI | GGATC | 6 cut(s) | 138, 588, 1112, 1271, 1284, 1623 |
| BspT104I | TTCGAA | 1 cut(s) | 665 |
| BspT107I | GGYRCC | 1 cut(s) | 50 |
| BspTI | CTTAAG | 1 cut(s) | 595 |
| BspTNI | GGTCTC | 1 cut(s) | 13 |
| BsrBI | CCGCTC | 2 cut(s) | 179, 441 |
| BsrFI | RCCGGY | 2 cut(s) | 47, 301 |
| BsrI | ACTGG | 4 cut(s) | 315, 382, 430, 609 |
| BssAI | RCCGGY | 2 cut(s) | 47, 301 |
| BssECI | CCNNGG | 4 cut(s) | 464, 465, 1075, 1463 |
| BssMI | GATC | 7 cut(s) | 143, 580, 842, 1054, 1117, 1276, 1628 |
| Bst2UI | CCWGG | 4 cut(s) | 466, 956, 1152, 1464 |
| Bst4CI | ACNGT | 6 cut(s) | 342, 838, 1076, 1376, 1499, 1737 |
| Bst6I | CTCTTC | 3 cut(s) | 233, 708, 991 |
| BstAFI | CTTAAG | 1 cut(s) | 595 |
| BstBI | TTCGAA | 1 cut(s) | 665 |
| BstC8I | GCNNGC | 7 cut(s) | 303, 656, 1039, 1165, 1453, 1545, 1661 |
| BstDEI | CTNAG | 4 cut(s) | 867, 1026, 1088, 1291 |
| BstDSI | CCRYGG | 1 cut(s) | 1075 |
| BstF5I | GGATG | 2 cut(s) | 1404, 1672 |
| BstFNI | CGCG | 1 cut(s) | 352 |
| BstH2I | RGCGCY | 1 cut(s) | 162 |
| BstHHI | GCGC | 2 cut(s) | 161, 1138 |
| BstKTI | GATC | 7 cut(s) | 146, 583, 845, 1057, 1120, 1279, 1631 |
| BstMAI | GTCTC | 3 cut(s) | 13, 210, 847 |
| BstMBI | GATC | 7 cut(s) | 143, 580, 842, 1054, 1117, 1276, 1628 |
| BstMCI | CGRYCG | 2 cut(s) | 344, 376 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 11, 469, 1508 |
| BstNI | CCWGG | 4 cut(s) | 466, 956, 1152, 1464 |
| BstNSI | RCATGY | 1 cut(s) | 641 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 399 |
| BstSCI | CCNGG | 5 cut(s) | 449, 464, 954, 1150, 1462 |
| BstUI | CGCG | 1 cut(s) | 352 |
| BstV1I | GCAGC | 3 cut(s) | 743, 831, 1682 |
| BstV2I | GAAGAC | 1 cut(s) | 681 |
| BstX2I | RGATCY | 5 cut(s) | 143, 1054, 1117, 1276, 1628 |
| BstYI | RGATCY | 5 cut(s) | 143, 1054, 1117, 1276, 1628 |
| BsuI | GTATCC | 1 cut(s) | 1243 |
| BsuRI | GGCC | 3 cut(s) | 301, 429, 1214 |
| BtgI | CCRYGG | 1 cut(s) | 1075 |
| BtgZI | GCGATG | 1 cut(s) | 1251 |
| BtrI | CACGTC | 1 cut(s) | 857 |
| BtsCI | GGATG | 2 cut(s) | 1404, 1672 |
| BtsI | GCAGTG | 3 cut(s) | 1462, 1625, 1750 |
| BtsIMutI | CAGTG | 6 cut(s) | 322, 423, 1462, 1504, 1625, 1750 |
| Cac8I | GCNNGC | 7 cut(s) | 303, 656, 1039, 1165, 1453, 1545, 1661 |
| CciI | TCATGA | 1 cut(s) | 1359 |
| CfoI | GCGC | 2 cut(s) | 161, 1138 |
| Cfr10I | RCCGGY | 2 cut(s) | 47, 301 |
| Cfr13I | GGNCC | 3 cut(s) | 171, 606, 1460 |
| CsiI | ACCWGGT | 1 cut(s) | 954 |
| Csp6I | GTAC | 3 cut(s) | 510, 1109, 1582 |
| CspAI | ACCGGT | 1 cut(s) | 47 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 529, 564 |
| CviAII | CATG | 4 cut(s) | 603, 638, 1360, 1609 |
| CviQI | GTAC | 3 cut(s) | 510, 1109, 1582 |
| DdeI | CTNAG | 4 cut(s) | 867, 1026, 1088, 1291 |
| DpnI | GATC | 7 cut(s) | 145, 582, 844, 1056, 1119, 1278, 1630 |
| DpnII | GATC | 7 cut(s) | 143, 580, 842, 1054, 1117, 1276, 1628 |
| DraI | TTTAAA | 1 cut(s) | 727 |
| EaeI | YGGCCR | 1 cut(s) | 427 |
| Eam1104I | CTCTTC | 3 cut(s) | 233, 708, 991 |
| EarI | CTCTTC | 3 cut(s) | 233, 708, 991 |
| Ecl136II | GAGCTC | 1 cut(s) | 1087 |
| Eco24I | GRGCYC | 2 cut(s) | 465, 1089 |
| Eco31I | GGTCTC | 1 cut(s) | 13 |
| Eco32I | GATATC | 2 cut(s) | 943, 1128 |
| Eco47I | GGWCC | 3 cut(s) | 171, 606, 1460 |
| Eco47III | AGCGCT | 1 cut(s) | 160 |
| Eco53kI | GAGCTC | 1 cut(s) | 1087 |
| Eco57I | CTGAAG | 4 cut(s) | 867, 1121, 1338, 1422 |
| Eco88I | CYCGRG | 1 cut(s) | 397 |
| EcoICRI | GAGCTC | 1 cut(s) | 1087 |
| EcoRII | CCWGG | 4 cut(s) | 464, 954, 1150, 1462 |
| EcoRV | GATATC | 2 cut(s) | 943, 1128 |
| EcoT38I | GRGCYC | 2 cut(s) | 465, 1089 |
| Esp3I | CGTCTC | 1 cut(s) | 847 |
| FaeI | CATG | 4 cut(s) | 606, 641, 1363, 1612 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1312, 1344 |
| FaqI | GGGAC | 1 cut(s) | 351 |
| FatI | CATG | 4 cut(s) | 602, 637, 1359, 1608 |
| FauI | CCCGC | 1 cut(s) | 279 |
| FblI | GTMKAC | 2 cut(s) | 236, 420 |
| Fnu4HI | GCNGC | 3 cut(s) | 732, 820, 1671 |
| FokI | GGATG | 2 cut(s) | 1391, 1679 |
| FriOI | GRGCYC | 2 cut(s) | 465, 1089 |
| Fsp4HI | GCNGC | 3 cut(s) | 732, 820, 1671 |
| FspBI | CTAG | 7 cut(s) | 275, 330, 564, 920, 1038, 1164, 1452 |
| GlaI | GCGC | 2 cut(s) | 160, 1137 |
| GluI | GCNGC | 3 cut(s) | 732, 820, 1671 |
| GsuI | CTGGAG | 1 cut(s) | 726 |
| HaeII | RGCGCY | 1 cut(s) | 162 |
| HaeIII | GGCC | 3 cut(s) | 301, 429, 1214 |
| HapII | CCGG | 7 cut(s) | 48, 302, 387, 451, 458, 768, 914 |
| HhaI | GCGC | 2 cut(s) | 161, 1138 |
| Hin1II | CATG | 4 cut(s) | 606, 641, 1363, 1612 |
| Hin6I | GCGC | 2 cut(s) | 159, 1136 |
| HinP1I | GCGC | 2 cut(s) | 159, 1136 |
| HincII | GTYRAC | 3 cut(s) | 259, 394, 421 |
| HindII | GTYRAC | 3 cut(s) | 259, 394, 421 |
| HpaII | CCGG | 7 cut(s) | 48, 302, 387, 451, 458, 768, 914 |
| HphI | GGTGA | 3 cut(s) | 612, 782, 1661 |
| Hpy166II | GTNNAC | 6 cut(s) | 237, 259, 394, 421, 740, 1079 |
| Hpy8I | GTNNAC | 6 cut(s) | 237, 259, 394, 421, 740, 1079 |
| Hpy99I | CGWCG | 1 cut(s) | 435 |
| HpyAV | CCTTC | 7 cut(s) | 327, 530, 583, 642, 894, 1096, 1446 |
| HpyCH4III | ACNGT | 6 cut(s) | 342, 838, 1076, 1376, 1499, 1737 |
| HpyCH4IV | ACGT | 2 cut(s) | 856, 1192 |
| HpyCH4V | TGCA | 6 cut(s) | 641, 658, 971, 1618, 1663, 1670 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 11, 469, 1508 |
| HpyF3I | CTNAG | 4 cut(s) | 867, 1026, 1088, 1291 |
| HpySE526I | ACGT | 2 cut(s) | 856, 1192 |
| Hsp92II | CATG | 4 cut(s) | 606, 641, 1363, 1612 |
| HspAI | GCGC | 2 cut(s) | 159, 1136 |
| Kpn2I | TCCGGA | 2 cut(s) | 386, 457 |
| KroI | GCCGGC | 1 cut(s) | 301 |
| KroNI | GCCGGC | 1 cut(s) | 303 |
| Kzo9I | GATC | 7 cut(s) | 143, 580, 842, 1054, 1117, 1276, 1628 |
| LmnI | GCTCC | 8 cut(s) | 90, 378, 405, 446, 460, 707, 1069, 1468 |
| Lsp1109I | GCAGC | 3 cut(s) | 743, 831, 1682 |
| LweI | GCATC | 2 cut(s) | 1413, 1657 |
| MabI | ACCWGGT | 1 cut(s) | 954 |
| MaeI | CTAG | 7 cut(s) | 275, 330, 564, 920, 1038, 1164, 1452 |
| MaeII | ACGT | 2 cut(s) | 856, 1192 |
| MaeIII | GTNAC | 1 cut(s) | 1640 |
| MalI | GATC | 7 cut(s) | 145, 582, 844, 1056, 1119, 1278, 1630 |
| MbiI | CCGCTC | 2 cut(s) | 179, 441 |
| MboI | GATC | 7 cut(s) | 143, 580, 842, 1054, 1117, 1276, 1628 |
| MflI | RGATCY | 5 cut(s) | 143, 1054, 1117, 1276, 1628 |
| MhlI | GDGCHC | 4 cut(s) | 95, 465, 1074, 1089 |
| MluCI | AATT | 7 cut(s) | 667, 754, 876, 977, 1415, 1523, 1613 |
| MlyI | GAGTC | 4 cut(s) | 179, 389, 426, 1727 |
| MmeI | TCCRAC | 3 cut(s) | 356, 383, 558 |
| MroI | TCCGGA | 2 cut(s) | 386, 457 |
| MroNI | GCCGGC | 1 cut(s) | 301 |
| MroXI | GAANNNNTTC | 2 cut(s) | 837, 1110 |
| MseI | TTAA | 3 cut(s) | 596, 726, 1302 |
| MslI | CAYNNNNRTG | 1 cut(s) | 768 |
| MspA1I | CMGCKG | 2 cut(s) | 254, 1547 |
| MspCI | CTTAAG | 1 cut(s) | 595 |
| MspI | CCGG | 7 cut(s) | 48, 302, 387, 451, 458, 768, 914 |
| MspR9I | CCNGG | 5 cut(s) | 451, 466, 956, 1152, 1464 |
| Mva1269I | GAATGC | 1 cut(s) | 616 |
| MvaI | CCWGG | 4 cut(s) | 466, 956, 1152, 1464 |
| MvnI | CGCG | 1 cut(s) | 352 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 11, 469, 1508 |
| NaeI | GCCGGC | 1 cut(s) | 303 |
| NciI | CCSGG | 1 cut(s) | 451 |
| NdeII | GATC | 7 cut(s) | 143, 580, 842, 1054, 1117, 1276, 1628 |
| NgoMIV | GCCGGC | 1 cut(s) | 301 |
| NheI | GCTAGC | 3 cut(s) | 1037, 1163, 1451 |
| NlaIII | CATG | 4 cut(s) | 606, 641, 1363, 1612 |
| NlaIV | GGNNCC | 7 cut(s) | 52, 380, 407, 462, 709, 1278, 1470 |
| NmuCI | GTSAC | 1 cut(s) | 1640 |
| NspI | RCATGY | 1 cut(s) | 641 |
| NspV | TTCGAA | 1 cut(s) | 665 |
| PagI | TCATGA | 1 cut(s) | 1359 |
| PasI | CCCWGGG | 1 cut(s) | 465 |
| PctI | GAATGC | 1 cut(s) | 616 |
| PdiI | GCCGGC | 1 cut(s) | 303 |
| PdmI | GAANNNNTTC | 2 cut(s) | 837, 1110 |
| PfeI | GAWTC | 6 cut(s) | 242, 293, 326, 871, 1095, 1480 |
| PflFI | GACNNNGTC | 1 cut(s) | 604 |
| PfoI | TCCNGGA | 1 cut(s) | 1150 |
| PinAI | ACCGGT | 1 cut(s) | 47 |
| PkrI | GCNGC | 3 cut(s) | 733, 821, 1672 |
| PleI | GAGTC | 4 cut(s) | 179, 389, 425, 1726 |
| PpsI | GAGTC | 4 cut(s) | 179, 389, 425, 1726 |
| PshAI | GACNNNNGTC | 1 cut(s) | 399 |
| PshBI | ATTAAT | 1 cut(s) | 1302 |
| Psp124BI | GAGCTC | 1 cut(s) | 1089 |
| Psp6I | CCWGG | 4 cut(s) | 464, 954, 1150, 1462 |
| PspGI | CCWGG | 4 cut(s) | 464, 954, 1150, 1462 |
| PspN4I | GGNNCC | 7 cut(s) | 52, 380, 407, 462, 709, 1278, 1470 |
| PspPI | GGNCC | 3 cut(s) | 171, 606, 1460 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1741, 1773 |
| PsuI | RGATCY | 5 cut(s) | 143, 1054, 1117, 1276, 1628 |
| PsyI | GACNNNGTC | 1 cut(s) | 604 |
| PvuII | CAGCTG | 1 cut(s) | 1547 |
| RsaI | GTAC | 3 cut(s) | 511, 1110, 1583 |
| RsaNI | GTAC | 3 cut(s) | 510, 1109, 1582 |
| RseI | CAYNNNNRTG | 1 cut(s) | 768 |
| SacI | GAGCTC | 1 cut(s) | 1089 |
| SalI | GTCGAC | 1 cut(s) | 419 |
| SaqAI | TTAA | 3 cut(s) | 596, 726, 1302 |
| SatI | GCNGC | 3 cut(s) | 732, 820, 1671 |
| Sau3AI | GATC | 7 cut(s) | 143, 580, 842, 1054, 1117, 1276, 1628 |
| Sau96I | GGNCC | 3 cut(s) | 171, 606, 1460 |
| ScaI | AGTACT | 1 cut(s) | 1110 |
| SchI | GAGTC | 4 cut(s) | 179, 389, 426, 1727 |
| ScrFI | CCNGG | 5 cut(s) | 451, 466, 956, 1152, 1464 |
| SduI | GDGCHC | 4 cut(s) | 95, 465, 1074, 1089 |
| SexAI | ACCWGGT | 1 cut(s) | 954 |
| SfaNI | GCATC | 2 cut(s) | 1413, 1657 |
| SfuI | TTCGAA | 1 cut(s) | 665 |
| SgrAI | CRCCGGYG | 1 cut(s) | 47 |
| SinI | GGWCC | 3 cut(s) | 171, 606, 1460 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 768 |
| SmlI | CTYRAG | 5 cut(s) | 166, 189, 595, 1016, 1556 |
| SmoI | CTYRAG | 5 cut(s) | 166, 189, 595, 1016, 1556 |
| Sse9I | AATT | 7 cut(s) | 667, 754, 876, 977, 1415, 1523, 1613 |
| SsiI | CCGC | 7 cut(s) | 5, 177, 252, 272, 352, 439, 690 |
| SspI | AATATT | 1 cut(s) | 199 |
| SspMI | CTAG | 7 cut(s) | 275, 330, 564, 920, 1038, 1164, 1452 |
| SstI | GAGCTC | 1 cut(s) | 1089 |
| StyD4I | CCNGG | 5 cut(s) | 449, 464, 954, 1150, 1462 |
| TaaI | ACNGT | 6 cut(s) | 342, 838, 1076, 1376, 1499, 1737 |
| TaiI | ACGT | 2 cut(s) | 859, 1195 |
| TaqI | TCGA | 6 cut(s) | 183, 296, 420, 665, 841, 1721 |
| TaqII | GACCGA | 1 cut(s) | 362 |
| TasI | AATT | 7 cut(s) | 667, 754, 876, 977, 1415, 1523, 1613 |
| TatI | WGTACW | 3 cut(s) | 509, 1108, 1581 |
| TfiI | GAWTC | 6 cut(s) | 242, 293, 326, 871, 1095, 1480 |
| Tru1I | TTAA | 3 cut(s) | 596, 726, 1302 |
| Tru9I | TTAA | 3 cut(s) | 596, 726, 1302 |
| TscAI | CASTG | 6 cut(s) | 322, 430, 1462, 1504, 1625, 1750 |
| TseFI | GTSAC | 1 cut(s) | 1640 |
| TseI | GCWGC | 3 cut(s) | 731, 819, 1670 |
| Tsp45I | GTSAC | 1 cut(s) | 1640 |
| TspDTI | ATGAA | 9 cut(s) | 54, 507, 513, 761, 785, 846, 1376, 1401, 1749 |
| TspGWI | ACGGA | 5 cut(s) | 103, 254, 433, 466, 693 |
| TspRI | CASTG | 6 cut(s) | 322, 430, 1462, 1504, 1625, 1750 |
| Tth111I | GACNNNGTC | 1 cut(s) | 604 |
| Vha464I | CTTAAG | 1 cut(s) | 595 |
| VpaK11BI | GGWCC | 3 cut(s) | 171, 606, 1460 |
| VspI | ATTAAT | 1 cut(s) | 1302 |
| XapI | RAATTY | 1 cut(s) | 1523 |
| XbaI | TCTAGA | 1 cut(s) | 329 |
| XceI | RCATGY | 1 cut(s) | 641 |
| XmiI | GTMKAC | 2 cut(s) | 236, 420 |
| XmnI | GAANNNNTTC | 2 cut(s) | 837, 1110 |
| XspI | CTAG | 7 cut(s) | 275, 330, 564, 920, 1038, 1164, 1452 |
| ZrmI | AGTACT | 1 cut(s) | 1110 |