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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 811, 2369 |
| AccII | CGCG | 2 cut(s) | 232, 1938 |
| AciI | CCGC | 5 cut(s) | 230, 710, 1520, 1611, 1850 |
| AclWI | GGATC | 6 cut(s) | 16, 450, 463, 2244, 2522, 2535 |
| AcoI | YGGCCR | 2 cut(s) | 1540, 1986 |
| AcsI | RAATTY | 2 cut(s) | 962, 2386 |
| AcuI | CTGAAG | 3 cut(s) | 1317, 1800, 2212 |
| AfaI | GTAC | 5 cut(s) | 213, 297, 893, 1004, 2259 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 735, 1402 |
| AflII | CTTAAG | 1 cut(s) | 2123 |
| AgsI | TTSAA | 9 cut(s) | 153, 237, 321, 871, 1211, 1796, 2158, 2317, 2391 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 1878 |
| AjiI | CACGTC | 2 cut(s) | 18, 2207 |
| AjnI | CCWGG | 4 cut(s) | 887, 1066, 1542, 2437 |
| Alw26I | GTCTC | 2 cut(s) | 100, 775 |
| AlwI | GGATC | 6 cut(s) | 16, 450, 463, 2244, 2522, 2535 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1910 |
| AoxI | GGCC | 6 cut(s) | 850, 1540, 1964, 1986, 2166, 2440 |
| ApeKI | GCWGC | 3 cut(s) | 125, 1055, 1847 |
| ApoI | RAATTY | 2 cut(s) | 962, 2386 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 413, 445 |
| AseI | ATTAAT | 1 cut(s) | 765 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 924 |
| AspLEI | GCGC | 4 cut(s) | 361, 468, 793, 1938 |
| AspS9I | GGNCC | 4 cut(s) | 380, 757, 1214, 2166 |
| AsuHPI | GGTGA | 3 cut(s) | 1494, 2242, 2506 |
| AsuII | TTCGAA | 1 cut(s) | 2534 |
| AvaII | GGWCC | 3 cut(s) | 380, 757, 1214 |
| BaeGI | GKGCMC | 2 cut(s) | 195, 2374 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 2268, 2301 |
| BalI | TGGCCA | 1 cut(s) | 1542 |
| BamHI | GGATCC | 2 cut(s) | 455, 2527 |
| BanI | GGYRCC | 2 cut(s) | 811, 2369 |
| BanII | GRGCYC | 1 cut(s) | 1414 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 2282, 2314 |
| BbsI | GAAGAC | 2 cut(s) | 62, 2514 |
| BbvI | GCAGC | 3 cut(s) | 112, 1042, 1834 |
| BccI | CCATC | 8 cut(s) | 613, 683, 1014, 1115, 1317, 1363, 1566, 2231 |
| BceAI | ACGGC | 3 cut(s) | 188, 272, 2231 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 2000, 2034 |
| BciT130I | CCWGG | 4 cut(s) | 889, 1068, 1544, 2439 |
| BcoDI | GTCTC | 2 cut(s) | 100, 775 |
| BfmI | CTRYAG | 2 cut(s) | 846, 1992 |
| BfoI | RGCGCY | 1 cut(s) | 794 |
| BfrI | CTTAAG | 1 cut(s) | 2123 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 1418 |
| BisI | GCNGC | 5 cut(s) | 126, 711, 1056, 1611, 1848 |
| BlpI | GCTNAGC | 1 cut(s) | 1326 |
| BlsI | GCNGC | 5 cut(s) | 127, 712, 1057, 1612, 1849 |
| Bme1390I | CCNGG | 4 cut(s) | 889, 1068, 1544, 2439 |
| Bme18I | GGWCC | 3 cut(s) | 380, 757, 1214 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 1878 |
| BmgBI | CACGTC | 2 cut(s) | 18, 2207 |
| BmgT120I | GGNCC | 4 cut(s) | 380, 757, 1214, 2166 |
| BmiI | GGNNCC | 9 cut(s) | 381, 457, 508, 696, 759, 813, 1786, 2371, 2529 |
| BmrFI | CCNGG | 4 cut(s) | 889, 1068, 1544, 2439 |
| BmrI | ACTGGG | 1 cut(s) | 273 |
| BmsI | GCATC | 9 cut(s) | 119, 262, 346, 425, 711, 851, 1611, 1930, 2407 |
| BmuI | ACTGGG | 1 cut(s) | 273 |
| BpiI | GAAGAC | 2 cut(s) | 62, 2514 |
| BpmI | CTGGAG | 1 cut(s) | 1460 |
| Bpu10I | CCTNAGC | 2 cut(s) | 714, 1710 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 1326 |
| Bpu14I | TTCGAA | 1 cut(s) | 2534 |
| BpuEI | CTTGAG | 2 cut(s) | 2152, 2282 |
| Bsa29I | ATCGAT | 1 cut(s) | 1494 |
| BsaBI | GATNNNNATC | 1 cut(s) | 945 |
| BsaI | GGTCTC | 1 cut(s) | 100 |
| BsaJI | CCNNGG | 5 cut(s) | 510, 753, 1067, 1543, 2374 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 710, 740 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 735, 1402 |
| Bse118I | RCCGGY | 1 cut(s) | 1984 |
| Bse1I | ACTGG | 5 cut(s) | 279, 698, 1056, 1443, 1904 |
| Bse3DI | GCAATG | 3 cut(s) | 827, 1473, 1956 |
| Bse8I | GATNNNNATC | 1 cut(s) | 945 |
| BseBI | CCWGG | 4 cut(s) | 889, 1068, 1544, 2439 |
| BseCI | ATCGAT | 1 cut(s) | 1494 |
| BseDI | CCNNGG | 5 cut(s) | 510, 753, 1067, 1543, 2374 |
| BseJI | GATNNNNATC | 1 cut(s) | 945 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 735, 1402 |
| BseMI | GCAATG | 3 cut(s) | 827, 1473, 1956 |
| BseMII | CTCAG | 7 cut(s) | 272, 356, 1049, 1350, 1724, 2385, 2481 |
| BseNI | ACTGG | 5 cut(s) | 279, 698, 1056, 1443, 1904 |
| BseRI | GAGGAG | 3 cut(s) | 1394, 1649, 1697 |
| BseSI | GKGCMC | 2 cut(s) | 195, 2374 |
| BseXI | GCAGC | 3 cut(s) | 112, 1042, 1834 |
| BseYI | CCCAGC | 1 cut(s) | 1699 |
| BsgI | GTGCAG | 1 cut(s) | 1166 |
| Bsh1236I | CGCG | 2 cut(s) | 232, 1938 |
| BshFI | GGCC | 6 cut(s) | 852, 1542, 1966, 1988, 2168, 2442 |
| BshNI | GGYRCC | 2 cut(s) | 811, 2369 |
| BshVI | ATCGAT | 1 cut(s) | 1494 |
| BsiSI | CCGG | 1 cut(s) | 1985 |
| BslFI | GGGAC | 5 cut(s) | 393, 743, 1892, 1963, 2484 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 735, 1402 |
| BsmAI | GTCTC | 2 cut(s) | 100, 775 |
| BsmFI | GGGAC | 5 cut(s) | 393, 743, 1892, 1963, 2484 |
| BsmI | GAATGC | 1 cut(s) | 1897 |
| BsnI | GGCC | 6 cut(s) | 852, 1542, 1966, 1988, 2168, 2442 |
| Bso31I | GGTCTC | 1 cut(s) | 100 |
| Bsp119I | TTCGAA | 1 cut(s) | 2534 |
| Bsp1286I | GDGCHC | 3 cut(s) | 195, 1414, 2374 |
| Bsp143I | GATC | 8 cut(s) | 8, 455, 480, 1528, 2236, 2340, 2405, 2527 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 1326 |
| BspACI | CCGC | 5 cut(s) | 230, 710, 1520, 1611, 1850 |
| BspANI | GGCC | 6 cut(s) | 852, 1542, 1966, 1988, 2168, 2442 |
| BspCNI | CTCAG | 7 cut(s) | 271, 355, 1048, 1349, 1723, 2386, 2480 |
| BspDI | ATCGAT | 1 cut(s) | 1494 |
| BspFNI | CGCG | 2 cut(s) | 232, 1938 |
| BspLI | GGNNCC | 9 cut(s) | 381, 457, 508, 696, 759, 813, 1786, 2371, 2529 |
| BspMAI | CTGCAG | 2 cut(s) | 850, 1996 |
| BspPI | GGATC | 6 cut(s) | 16, 450, 463, 2244, 2522, 2535 |
| BspQI | GCTCTTC | 1 cut(s) | 1179 |
| BspT104I | TTCGAA | 1 cut(s) | 2534 |
| BspT107I | GGYRCC | 2 cut(s) | 811, 2369 |
| BspTI | CTTAAG | 1 cut(s) | 2123 |
| BspTNI | GGTCTC | 1 cut(s) | 100 |
| BsrDI | GCAATG | 3 cut(s) | 827, 1473, 1956 |
| BsrFI | RCCGGY | 1 cut(s) | 1984 |
| BsrI | ACTGG | 5 cut(s) | 279, 698, 1056, 1443, 1904 |
| BssAI | RCCGGY | 1 cut(s) | 1984 |
| BssECI | CCNNGG | 5 cut(s) | 510, 753, 1067, 1543, 2374 |
| BssMI | GATC | 8 cut(s) | 8, 455, 480, 1528, 2236, 2340, 2405, 2527 |
| BssT1I | CCWWGG | 3 cut(s) | 510, 753, 2374 |
| Bst2UI | CCWGG | 4 cut(s) | 889, 1068, 1544, 2439 |
| Bst4CI | ACNGT | 4 cut(s) | 1234, 1678, 1732, 2491 |
| Bst6I | CTCTTC | 2 cut(s) | 1179, 2531 |
| BstAFI | CTTAAG | 1 cut(s) | 2123 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 116 |
| BstBI | TTCGAA | 1 cut(s) | 2534 |
| BstC8I | GCNNGC | 6 cut(s) | 748, 850, 878, 1572, 1643, 2013 |
| BstFNI | CGCG | 2 cut(s) | 232, 1938 |
| BstH2I | RGCGCY | 1 cut(s) | 794 |
| BstHHI | GCGC | 4 cut(s) | 361, 468, 793, 1938 |
| BstKTI | GATC | 8 cut(s) | 11, 458, 483, 1531, 2239, 2343, 2408, 2530 |
| BstMAI | GTCTC | 2 cut(s) | 100, 775 |
| BstMBI | GATC | 8 cut(s) | 8, 455, 480, 1528, 2236, 2340, 2405, 2527 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 116, 125, 1418, 2012, 2119 |
| BstNI | CCWGG | 4 cut(s) | 889, 1068, 1544, 2439 |
| BstNSI | RCATGY | 2 cut(s) | 445, 1697 |
| BstSCI | CCNGG | 4 cut(s) | 887, 1066, 1542, 2437 |
| BstSFI | CTRYAG | 2 cut(s) | 846, 1992 |
| BstSLI | GKGCMC | 2 cut(s) | 195, 2374 |
| BstUI | CGCG | 2 cut(s) | 232, 1938 |
| BstV1I | GCAGC | 3 cut(s) | 112, 1042, 1834 |
| BstV2I | GAAGAC | 2 cut(s) | 62, 2514 |
| BstX2I | RGATCY | 4 cut(s) | 8, 455, 2236, 2527 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 822 |
| BstYI | RGATCY | 4 cut(s) | 8, 455, 2236, 2527 |
| Bsu15I | ATCGAT | 1 cut(s) | 1494 |
| BsuRI | GGCC | 6 cut(s) | 852, 1542, 1966, 1988, 2168, 2442 |
| BsuTUI | ATCGAT | 1 cut(s) | 1494 |
| BtgZI | GCGATG | 2 cut(s) | 591, 1953 |
| BtrI | CACGTC | 2 cut(s) | 18, 2207 |
| BtsI | GCAGTG | 2 cut(s) | 1989, 2054 |
| BtsIMutI | CAGTG | 5 cut(s) | 1436, 1728, 1989, 2054, 2556 |
| Cac8I | GCNNGC | 6 cut(s) | 748, 850, 878, 1572, 1643, 2013 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1910 |
| CfoI | GCGC | 4 cut(s) | 361, 468, 793, 1938 |
| Cfr10I | RCCGGY | 1 cut(s) | 1984 |
| Cfr13I | GGNCC | 4 cut(s) | 380, 757, 1214, 2166 |
| ClaI | ATCGAT | 1 cut(s) | 1494 |
| CsiI | ACCWGGT | 1 cut(s) | 887 |
| Csp6I | GTAC | 5 cut(s) | 212, 296, 892, 1003, 2258 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1978, 2013 |
| CviAII | CATG | 5 cut(s) | 442, 1694, 1832, 2338, 2524 |
| CviQI | GTAC | 5 cut(s) | 212, 296, 892, 1003, 2258 |
| DpnI | GATC | 8 cut(s) | 10, 457, 482, 1530, 2238, 2342, 2407, 2529 |
| DpnII | GATC | 8 cut(s) | 8, 455, 480, 1528, 2236, 2340, 2405, 2527 |
| DraI | TTTAAA | 1 cut(s) | 2425 |
| DriI | GACNNNNNGTC | 1 cut(s) | 1878 |
| EaeI | YGGCCR | 2 cut(s) | 1540, 1986 |
| Eam1104I | CTCTTC | 2 cut(s) | 1179, 2531 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 1878 |
| EarI | CTCTTC | 2 cut(s) | 1179, 2531 |
| Eco130I | CCWWGG | 3 cut(s) | 510, 753, 2374 |
| Eco24I | GRGCYC | 1 cut(s) | 1414 |
| Eco31I | GGTCTC | 1 cut(s) | 100 |
| Eco32I | GATATC | 1 cut(s) | 2474 |
| Eco47I | GGWCC | 3 cut(s) | 380, 757, 1214 |
| Eco57I | CTGAAG | 3 cut(s) | 1317, 1800, 2212 |
| EcoO109I | RGGNCCY | 2 cut(s) | 757, 1214 |
| EcoRI | GAATTC | 1 cut(s) | 962 |
| EcoRII | CCWGG | 4 cut(s) | 887, 1066, 1542, 2437 |
| EcoRV | GATATC | 1 cut(s) | 2474 |
| EcoT14I | CCWWGG | 3 cut(s) | 510, 753, 2374 |
| EcoT38I | GRGCYC | 1 cut(s) | 1414 |
| ErhI | CCWWGG | 3 cut(s) | 510, 753, 2374 |
| FaeI | CATG | 5 cut(s) | 445, 1697, 1835, 2341, 2527 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 787, 819, 1455, 1487 |
| FaqI | GGGAC | 5 cut(s) | 393, 743, 1892, 1963, 2484 |
| FatI | CATG | 5 cut(s) | 441, 1693, 1831, 2337, 2523 |
| FauI | CCCGC | 1 cut(s) | 1527 |
| FauNDI | CATATG | 2 cut(s) | 1030, 2138 |
| Fnu4HI | GCNGC | 5 cut(s) | 126, 711, 1056, 1611, 1848 |
| FriOI | GRGCYC | 1 cut(s) | 1414 |
| Fsp4HI | GCNGC | 5 cut(s) | 126, 711, 1056, 1611, 1848 |
| GlaI | GCGC | 4 cut(s) | 360, 467, 792, 1937 |
| GluI | GCNGC | 5 cut(s) | 126, 711, 1056, 1611, 1848 |
| GsaI | CCCAGC | 1 cut(s) | 1703 |
| GsuI | CTGGAG | 1 cut(s) | 1460 |
| HaeII | RGCGCY | 1 cut(s) | 794 |
| HaeIII | GGCC | 6 cut(s) | 852, 1542, 1966, 1988, 2168, 2442 |
| HapII | CCGG | 1 cut(s) | 1985 |
| HhaI | GCGC | 4 cut(s) | 361, 468, 793, 1938 |
| Hin1II | CATG | 5 cut(s) | 445, 1697, 1835, 2341, 2527 |
| Hin6I | GCGC | 4 cut(s) | 359, 466, 791, 1936 |
| HinP1I | GCGC | 4 cut(s) | 359, 466, 791, 1936 |
| HincII | GTYRAC | 1 cut(s) | 2353 |
| HindII | GTYRAC | 1 cut(s) | 2353 |
| HindIII | AAGCTT | 2 cut(s) | 801, 1328 |
| HinfI | GANTC | 7 cut(s) | 631, 911, 1499, 1738, 1808, 2321, 2497 |
| HpaII | CCGG | 1 cut(s) | 1985 |
| HphI | GGTGA | 3 cut(s) | 1494, 2242, 2506 |
| Hpy166II | GTNNAC | 3 cut(s) | 146, 596, 2353 |
| Hpy188I | TCNGA | 8 cut(s) | 1230, 1339, 1583, 1654, 1862, 2204, 2231, 2416 |
| Hpy8I | GTNNAC | 3 cut(s) | 146, 596, 2353 |
| HpyCH4III | ACNGT | 4 cut(s) | 1234, 1678, 1732, 2491 |
| HpyCH4IV | ACGT | 6 cut(s) | 17, 79, 148, 1321, 2206, 2550 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 116, 125, 1418, 2012, 2119 |
| HpySE526I | ACGT | 6 cut(s) | 17, 79, 148, 1321, 2206, 2550 |
| Hsp92II | CATG | 5 cut(s) | 445, 1697, 1835, 2341, 2527 |
| HspAI | GCGC | 4 cut(s) | 359, 466, 791, 1936 |
| Kzo9I | GATC | 8 cut(s) | 8, 455, 480, 1528, 2236, 2340, 2405, 2527 |
| LguI | GCTCTTC | 1 cut(s) | 1179 |
| Lsp1109I | GCAGC | 3 cut(s) | 112, 1042, 1834 |
| LweI | GCATC | 9 cut(s) | 119, 262, 346, 425, 711, 851, 1611, 1930, 2407 |
| MabI | ACCWGGT | 1 cut(s) | 887 |
| MaeII | ACGT | 6 cut(s) | 17, 79, 148, 1321, 2206, 2550 |
| MaeIII | GTNAC | 8 cut(s) | 586, 1500, 1513, 1657, 1870, 2248, 2362, 2551 |
| MalI | GATC | 8 cut(s) | 10, 457, 482, 1530, 2238, 2342, 2407, 2529 |
| MboI | GATC | 8 cut(s) | 8, 455, 480, 1528, 2236, 2340, 2405, 2527 |
| MflI | RGATCY | 4 cut(s) | 8, 455, 2236, 2527 |
| MhlI | GDGCHC | 3 cut(s) | 195, 1414, 2374 |
| MlsI | TGGCCA | 1 cut(s) | 1542 |
| MluCI | AATT | 8 cut(s) | 448, 762, 962, 1076, 1164, 1172, 2195, 2386 |
| MluNI | TGGCCA | 1 cut(s) | 1542 |
| MlyI | GAGTC | 4 cut(s) | 640, 1508, 1747, 2506 |
| MmeI | TCCRAC | 4 cut(s) | 288, 1561, 1632, 2141 |
| Mox20I | TGGCCA | 1 cut(s) | 1542 |
| MroXI | GAANNNNTTC | 1 cut(s) | 924 |
| MscI | TGGCCA | 1 cut(s) | 1542 |
| MseI | TTAA | 9 cut(s) | 71, 639, 675, 765, 771, 2124, 2304, 2424, 2486 |
| MslI | CAYNNNNRTG | 2 cut(s) | 737, 2285 |
| Msp20I | TGGCCA | 1 cut(s) | 1542 |
| MspCI | CTTAAG | 1 cut(s) | 2123 |
| MspI | CCGG | 1 cut(s) | 1985 |
| MspR9I | CCNGG | 4 cut(s) | 889, 1068, 1544, 2439 |
| Mva1269I | GAATGC | 1 cut(s) | 1897 |
| MvaI | CCWGG | 4 cut(s) | 889, 1068, 1544, 2439 |
| MvnI | CGCG | 2 cut(s) | 232, 1938 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 116, 125, 1418, 2012, 2119 |
| NdeI | CATATG | 2 cut(s) | 1030, 2138 |
| NdeII | GATC | 8 cut(s) | 8, 455, 480, 1528, 2236, 2340, 2405, 2527 |
| NlaIII | CATG | 5 cut(s) | 445, 1697, 1835, 2341, 2527 |
| NlaIV | GGNNCC | 9 cut(s) | 381, 457, 508, 696, 759, 813, 1786, 2371, 2529 |
| NmuCI | GTSAC | 6 cut(s) | 586, 1500, 1513, 1870, 2248, 2362 |
| NspI | RCATGY | 2 cut(s) | 445, 1697 |
| NspV | TTCGAA | 1 cut(s) | 2534 |
| PciSI | GCTCTTC | 1 cut(s) | 1179 |
| PctI | GAATGC | 1 cut(s) | 1897 |
| PdmI | GAANNNNTTC | 1 cut(s) | 924 |
| PfeI | GAWTC | 3 cut(s) | 911, 1808, 2321 |
| PkrI | GCNGC | 5 cut(s) | 127, 712, 1057, 1612, 1849 |
| PleI | GAGTC | 4 cut(s) | 639, 1507, 1746, 2505 |
| PpsI | GAGTC | 4 cut(s) | 639, 1507, 1746, 2505 |
| PpuMI | RGGWCCY | 2 cut(s) | 757, 1214 |
| PshBI | ATTAAT | 1 cut(s) | 765 |
| Psp5II | RGGWCCY | 2 cut(s) | 757, 1214 |
| Psp6I | CCWGG | 4 cut(s) | 887, 1066, 1542, 2437 |
| PspFI | CCCAGC | 1 cut(s) | 1699 |
| PspGI | CCWGG | 4 cut(s) | 887, 1066, 1542, 2437 |
| PspN4I | GGNNCC | 9 cut(s) | 381, 457, 508, 696, 759, 813, 1786, 2371, 2529 |
| PspPI | GGNCC | 4 cut(s) | 380, 757, 1214, 2166 |
| PspPPI | RGGWCCY | 2 cut(s) | 757, 1214 |
| PstI | CTGCAG | 2 cut(s) | 850, 1996 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1910 |
| PsuI | RGATCY | 4 cut(s) | 8, 455, 2236, 2527 |
| RsaI | GTAC | 5 cut(s) | 213, 297, 893, 1004, 2259 |
| RsaNI | GTAC | 5 cut(s) | 212, 296, 892, 1003, 2258 |
| RseI | CAYNNNNRTG | 2 cut(s) | 737, 2285 |
| SapI | GCTCTTC | 1 cut(s) | 1179 |
| SaqAI | TTAA | 9 cut(s) | 71, 639, 675, 765, 771, 2124, 2304, 2424, 2486 |
| SatI | GCNGC | 5 cut(s) | 126, 711, 1056, 1611, 1848 |
| Sau3AI | GATC | 8 cut(s) | 8, 455, 480, 1528, 2236, 2340, 2405, 2527 |
| Sau96I | GGNCC | 4 cut(s) | 380, 757, 1214, 2166 |
| SchI | GAGTC | 4 cut(s) | 640, 1508, 1747, 2506 |
| ScrFI | CCNGG | 4 cut(s) | 889, 1068, 1544, 2439 |
| SduI | GDGCHC | 3 cut(s) | 195, 1414, 2374 |
| SexAI | ACCWGGT | 1 cut(s) | 887 |
| SfaNI | GCATC | 9 cut(s) | 119, 262, 346, 425, 711, 851, 1611, 1930, 2407 |
| SfcI | CTRYAG | 2 cut(s) | 846, 1992 |
| SfuI | TTCGAA | 1 cut(s) | 2534 |
| SinI | GGWCC | 3 cut(s) | 380, 757, 1214 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 737, 2285 |
| SmlI | CTYRAG | 3 cut(s) | 2123, 2131, 2261 |
| SmoI | CTYRAG | 3 cut(s) | 2123, 2131, 2261 |
| Sse9I | AATT | 8 cut(s) | 448, 762, 962, 1076, 1164, 1172, 2195, 2386 |
| SsiI | CCGC | 5 cut(s) | 230, 710, 1520, 1611, 1850 |
| SspI | AATATT | 1 cut(s) | 1256 |
| StyD4I | CCNGG | 4 cut(s) | 887, 1066, 1542, 2437 |
| StyI | CCWWGG | 3 cut(s) | 510, 753, 2374 |
| TaaI | ACNGT | 4 cut(s) | 1234, 1678, 1732, 2491 |
| TaiI | ACGT | 6 cut(s) | 20, 82, 151, 1324, 2209, 2553 |
| TaqI | TCGA | 8 cut(s) | 949, 1012, 1084, 1158, 1494, 2324, 2404, 2534 |
| TasI | AATT | 8 cut(s) | 448, 762, 962, 1076, 1164, 1172, 2195, 2386 |
| TatI | WGTACW | 1 cut(s) | 2257 |
| TauI | GCSGC | 2 cut(s) | 713, 1613 |
| TfiI | GAWTC | 3 cut(s) | 911, 1808, 2321 |
| Tru1I | TTAA | 9 cut(s) | 71, 639, 675, 765, 771, 2124, 2304, 2424, 2486 |
| Tru9I | TTAA | 9 cut(s) | 71, 639, 675, 765, 771, 2124, 2304, 2424, 2486 |
| TscAI | CASTG | 5 cut(s) | 1443, 1735, 1996, 2054, 2563 |
| TseFI | GTSAC | 6 cut(s) | 586, 1500, 1513, 1870, 2248, 2362 |
| TseI | GCWGC | 3 cut(s) | 125, 1055, 1847 |
| Tsp45I | GTSAC | 6 cut(s) | 586, 1500, 1513, 1870, 2248, 2362 |
| TspDTI | ATGAA | 9 cut(s) | 68, 180, 549, 555, 1176, 1605, 1749, 1820, 2322 |
| TspGWI | ACGGA | 2 cut(s) | 347, 891 |
| TspRI | CASTG | 5 cut(s) | 1443, 1735, 1996, 2054, 2563 |
| Vha464I | CTTAAG | 1 cut(s) | 2123 |
| VpaK11BI | GGWCC | 3 cut(s) | 380, 757, 1214 |
| VspI | ATTAAT | 1 cut(s) | 765 |
| XapI | RAATTY | 2 cut(s) | 962, 2386 |
| XceI | RCATGY | 2 cut(s) | 445, 1697 |
| XcmI | CCANNNNNNNNNTGG | 3 cut(s) | 409, 1795, 1882 |
| XmnI | GAANNNNTTC | 1 cut(s) | 924 |