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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 1605 |
| Acc65I | GGTACC | 2 cut(s) | 1324, 1578 |
| AccB1I | GGYRCC | 3 cut(s) | 1324, 1474, 1578 |
| AccI | GTMKAC | 2 cut(s) | 1041, 1425 |
| AccII | CGCG | 1 cut(s) | 1304 |
| AciI | CCGC | 5 cut(s) | 145, 421, 1112, 1302, 1910 |
| AclWI | GGATC | 2 cut(s) | 88, 1232 |
| AcsI | RAATTY | 1 cut(s) | 1903 |
| AcuI | CTGAAG | 3 cut(s) | 96, 161, 348 |
| AcvI | CACGTG | 1 cut(s) | 353 |
| AdeI | CACNNNGTG | 1 cut(s) | 1271 |
| AfaI | GTAC | 4 cut(s) | 1092, 1326, 1571, 1580 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 542 |
| AflII | CTTAAG | 2 cut(s) | 131, 1811 |
| AflIII | ACRYGT | 2 cut(s) | 352, 862 |
| AjnI | CCWGG | 2 cut(s) | 1156, 1717 |
| AluBI | AGCT | 6 cut(s) | 136, 202, 621, 926, 1168, 1461 |
| AluI | AGCT | 6 cut(s) | 136, 202, 621, 926, 1168, 1461 |
| Alw21I | GWGCWC | 2 cut(s) | 543, 1264 |
| Alw26I | GTCTC | 2 cut(s) | 1064, 1552 |
| AlwI | GGATC | 2 cut(s) | 88, 1232 |
| Ama87I | CYCGRG | 1 cut(s) | 1747 |
| AoxI | GGCC | 4 cut(s) | 1002, 1680, 1720, 1879 |
| ApaI | GGGCCC | 2 cut(s) | 1006, 1684 |
| ApeKI | GCWGC | 2 cut(s) | 136, 1173 |
| ApoI | RAATTY | 1 cut(s) | 1903 |
| ArsI | GACNNNNNNTTYG | 2 cut(s) | 28, 60 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 883 |
| Asp718I | GGTACC | 2 cut(s) | 1324, 1578 |
| AspLEI | GCGC | 2 cut(s) | 390, 1306 |
| AspS9I | GGNCC | 5 cut(s) | 1002, 1003, 1680, 1681, 1879 |
| AsuHPI | GGTGA | 4 cut(s) | 1146, 1283, 1912, 1924 |
| AsuII | TTCGAA | 1 cut(s) | 89 |
| AvaI | CYCGRG | 1 cut(s) | 1747 |
| BaeGI | GKGCMC | 2 cut(s) | 1006, 1684 |
| BanI | GGYRCC | 3 cut(s) | 1324, 1474, 1578 |
| BanII | GRGCYC | 2 cut(s) | 1006, 1684 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 804, 836 |
| BbrPI | CACGTG | 1 cut(s) | 353 |
| BbsI | GAAGAC | 4 cut(s) | 32, 41, 948, 1809 |
| Bbv12I | GWGCWC | 2 cut(s) | 543, 1264 |
| BbvI | GCAGC | 2 cut(s) | 123, 1160 |
| BccI | CCATC | 4 cut(s) | 830, 953, 1268, 1301 |
| BceAI | ACGGC | 1 cut(s) | 339 |
| BciT130I | CCWGG | 2 cut(s) | 1158, 1719 |
| BclI | TGATCA | 1 cut(s) | 898 |
| BcoDI | GTCTC | 2 cut(s) | 1064, 1552 |
| BfaI | CTAG | 3 cut(s) | 870, 927, 1268 |
| BfrI | CTTAAG | 2 cut(s) | 131, 1811 |
| BisI | GCNGC | 4 cut(s) | 137, 146, 1112, 1174 |
| BlsI | GCNGC | 4 cut(s) | 138, 147, 1113, 1175 |
| Bme1390I | CCNGG | 2 cut(s) | 1158, 1719 |
| BmeT110I | CYCGRG | 1 cut(s) | 1747 |
| BmgT120I | GGNCC | 5 cut(s) | 1002, 1003, 1680, 1681, 1879 |
| BmiI | GGNNCC | 7 cut(s) | 291, 1004, 1110, 1326, 1476, 1580, 1682 |
| BmrFI | CCNGG | 2 cut(s) | 1158, 1719 |
| BmrI | ACTGGG | 1 cut(s) | 132 |
| BmsI | GCATC | 3 cut(s) | 307, 445, 1491 |
| BmuI | ACTGGG | 1 cut(s) | 132 |
| BpiI | GAAGAC | 4 cut(s) | 32, 41, 948, 1809 |
| BpmI | CTGGAG | 1 cut(s) | 501 |
| Bpu10I | CCTNAGC | 3 cut(s) | 246, 537, 1056 |
| Bpu14I | TTCGAA | 1 cut(s) | 89 |
| BpuEI | CTTGAG | 2 cut(s) | 816, 894 |
| BsaAI | YACGTR | 2 cut(s) | 353, 1138 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 434, 464 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 542 |
| Bse1I | ACTGG | 3 cut(s) | 127, 484, 1591 |
| Bse3DI | GCAATG | 1 cut(s) | 1476 |
| BseBI | CCWGG | 2 cut(s) | 1158, 1719 |
| BseGI | GGATG | 5 cut(s) | 460, 964, 1066, 1438, 1522 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 542 |
| BseMI | GCAATG | 1 cut(s) | 1476 |
| BseMII | CTCAG | 3 cut(s) | 528, 869, 1458 |
| BseNI | ACTGG | 3 cut(s) | 127, 484, 1591 |
| BseRI | GAGGAG | 4 cut(s) | 680, 843, 1136, 1139 |
| BseSI | GKGCMC | 2 cut(s) | 1006, 1684 |
| BseXI | GCAGC | 2 cut(s) | 123, 1160 |
| BsgI | GTGCAG | 1 cut(s) | 668 |
| Bsh1236I | CGCG | 1 cut(s) | 1304 |
| BshFI | GGCC | 4 cut(s) | 1004, 1682, 1722, 1881 |
| BshNI | GGYRCC | 3 cut(s) | 1324, 1474, 1578 |
| BsiHKAI | GWGCWC | 2 cut(s) | 543, 1264 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1747 |
| BsiSI | CCGG | 1 cut(s) | 1882 |
| BslFI | GGGAC | 2 cut(s) | 403, 544 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 542 |
| BsmAI | GTCTC | 2 cut(s) | 1064, 1552 |
| BsmFI | GGGAC | 2 cut(s) | 403, 544 |
| BsnI | GGCC | 4 cut(s) | 1004, 1682, 1722, 1881 |
| BsoBI | CYCGRG | 1 cut(s) | 1747 |
| Bsp119I | TTCGAA | 1 cut(s) | 89 |
| Bsp120I | GGGCCC | 2 cut(s) | 1002, 1680 |
| Bsp1286I | GDGCHC | 4 cut(s) | 543, 1006, 1264, 1684 |
| Bsp143I | GATC | 3 cut(s) | 80, 898, 1237 |
| BspACI | CCGC | 5 cut(s) | 145, 421, 1112, 1302, 1910 |
| BspANI | GGCC | 4 cut(s) | 1004, 1682, 1722, 1881 |
| BspCNI | CTCAG | 3 cut(s) | 529, 868, 1457 |
| BspFNI | CGCG | 1 cut(s) | 1304 |
| BspLI | GGNNCC | 7 cut(s) | 291, 1004, 1110, 1326, 1476, 1580, 1682 |
| BspPI | GGATC | 2 cut(s) | 88, 1232 |
| BspT104I | TTCGAA | 1 cut(s) | 89 |
| BspT107I | GGYRCC | 3 cut(s) | 1324, 1474, 1578 |
| BspTI | CTTAAG | 2 cut(s) | 131, 1811 |
| BsrDI | GCAATG | 1 cut(s) | 1476 |
| BsrI | ACTGG | 3 cut(s) | 127, 484, 1591 |
| BssMI | GATC | 3 cut(s) | 80, 898, 1237 |
| Bst2UI | CCWGG | 2 cut(s) | 1158, 1719 |
| Bst4CI | ACNGT | 6 cut(s) | 417, 634, 953, 1222, 1357, 1675 |
| Bst6I | CTCTTC | 4 cut(s) | 324, 441, 544, 1403 |
| BstAFI | CTTAAG | 2 cut(s) | 131, 1811 |
| BstBAI | YACGTR | 2 cut(s) | 353, 1138 |
| BstBI | TTCGAA | 1 cut(s) | 89 |
| BstC8I | GCNNGC | 3 cut(s) | 150, 1365, 1534 |
| BstDEI | CTNAG | 9 cut(s) | 9, 30, 99, 246, 537, 622, 855, 1056, 1444 |
| BstF5I | GGATG | 5 cut(s) | 460, 964, 1066, 1438, 1522 |
| BstFNI | CGCG | 1 cut(s) | 1304 |
| BstHHI | GCGC | 2 cut(s) | 390, 1306 |
| BstKTI | GATC | 3 cut(s) | 83, 901, 1240 |
| BstMAI | GTCTC | 2 cut(s) | 1064, 1552 |
| BstMBI | GATC | 3 cut(s) | 80, 898, 1237 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 142, 145 |
| BstNI | CCWGG | 2 cut(s) | 1158, 1719 |
| BstNSI | RCATGY | 4 cut(s) | 152, 827, 866, 1289 |
| BstSCI | CCNGG | 2 cut(s) | 1156, 1717 |
| BstSLI | GKGCMC | 2 cut(s) | 1006, 1684 |
| BstUI | CGCG | 1 cut(s) | 1304 |
| BstV1I | GCAGC | 2 cut(s) | 123, 1160 |
| BstV2I | GAAGAC | 4 cut(s) | 32, 41, 948, 1809 |
| BstX2I | RGATCY | 1 cut(s) | 80 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 1225 |
| BstYI | RGATCY | 1 cut(s) | 80 |
| BsuRI | GGCC | 4 cut(s) | 1004, 1682, 1722, 1881 |
| BtgZI | GCGATG | 1 cut(s) | 1248 |
| BtsCI | GGATG | 5 cut(s) | 460, 964, 1066, 1438, 1522 |
| BtsI | GCAGTG | 1 cut(s) | 159 |
| BtsIMutI | CAGTG | 2 cut(s) | 159, 1218 |
| Cac8I | GCNNGC | 3 cut(s) | 150, 1365, 1534 |
| CfoI | GCGC | 2 cut(s) | 390, 1306 |
| Cfr13I | GGNCC | 5 cut(s) | 1002, 1003, 1680, 1681, 1879 |
| CseI | GACGC | 2 cut(s) | 42, 698 |
| Csp6I | GTAC | 4 cut(s) | 1091, 1325, 1570, 1579 |
| CviQI | GTAC | 4 cut(s) | 1091, 1325, 1570, 1579 |
| DdeI | CTNAG | 9 cut(s) | 9, 30, 99, 246, 537, 622, 855, 1056, 1444 |
| DpnI | GATC | 3 cut(s) | 82, 900, 1239 |
| DpnII | GATC | 3 cut(s) | 80, 898, 1237 |
| DraIII | CACNNNGTG | 1 cut(s) | 1271 |
| Eam1104I | CTCTTC | 4 cut(s) | 324, 441, 544, 1403 |
| EarI | CTCTTC | 4 cut(s) | 324, 441, 544, 1403 |
| Eco24I | GRGCYC | 2 cut(s) | 1006, 1684 |
| Eco32I | GATATC | 1 cut(s) | 511 |
| Eco57I | CTGAAG | 3 cut(s) | 96, 161, 348 |
| Eco72I | CACGTG | 1 cut(s) | 353 |
| Eco88I | CYCGRG | 1 cut(s) | 1747 |
| EcoO109I | RGGNCCY | 3 cut(s) | 1002, 1003, 1680 |
| EcoRII | CCWGG | 2 cut(s) | 1156, 1717 |
| EcoRV | GATATC | 1 cut(s) | 511 |
| EcoT38I | GRGCYC | 2 cut(s) | 1006, 1684 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 605, 637 |
| FaqI | GGGAC | 2 cut(s) | 403, 544 |
| FauI | CCCGC | 1 cut(s) | 428 |
| FbaI | TGATCA | 1 cut(s) | 898 |
| FblI | GTMKAC | 2 cut(s) | 1041, 1425 |
| Fnu4HI | GCNGC | 4 cut(s) | 137, 146, 1112, 1174 |
| FokI | GGATG | 5 cut(s) | 467, 971, 1073, 1445, 1529 |
| FriOI | GRGCYC | 2 cut(s) | 1006, 1684 |
| Fsp4HI | GCNGC | 4 cut(s) | 137, 146, 1112, 1174 |
| FspBI | CTAG | 3 cut(s) | 870, 927, 1268 |
| GlaI | GCGC | 2 cut(s) | 389, 1305 |
| GluI | GCNGC | 4 cut(s) | 137, 146, 1112, 1174 |
| GsuI | CTGGAG | 1 cut(s) | 501 |
| HaeIII | GGCC | 4 cut(s) | 1004, 1682, 1722, 1881 |
| HapII | CCGG | 1 cut(s) | 1882 |
| HgaI | GACGC | 2 cut(s) | 42, 698 |
| HhaI | GCGC | 2 cut(s) | 390, 1306 |
| Hin6I | GCGC | 2 cut(s) | 388, 1304 |
| HinP1I | GCGC | 2 cut(s) | 388, 1304 |
| HincII | GTYRAC | 6 cut(s) | 57, 949, 1042, 1426, 1596, 1633 |
| HindII | GTYRAC | 6 cut(s) | 57, 949, 1042, 1426, 1596, 1633 |
| HinfI | GANTC | 7 cut(s) | 116, 184, 532, 577, 1043, 1442, 1490 |
| HpaI | GTTAAC | 1 cut(s) | 949 |
| HpaII | CCGG | 1 cut(s) | 1882 |
| HphI | GGTGA | 4 cut(s) | 1146, 1283, 1912, 1924 |
| Hpy166II | GTNNAC | 9 cut(s) | 57, 887, 949, 1042, 1426, 1570, 1596, 1615, 1633 |
| Hpy188I | TCNGA | 3 cut(s) | 76, 858, 1447 |
| Hpy188III | TCNNGA | 8 cut(s) | 359, 514, 536, 833, 911, 1014, 1551, 1641 |
| Hpy8I | GTNNAC | 9 cut(s) | 57, 887, 949, 1042, 1426, 1570, 1596, 1615, 1633 |
| HpyAV | CCTTC | 8 cut(s) | 71, 232, 276, 280, 1015, 1647, 1780, 1870 |
| HpyCH4III | ACNGT | 6 cut(s) | 417, 634, 953, 1222, 1357, 1675 |
| HpyCH4IV | ACGT | 4 cut(s) | 352, 607, 945, 1137 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 142, 145 |
| HpyF3I | CTNAG | 9 cut(s) | 9, 30, 99, 246, 537, 622, 855, 1056, 1444 |
| HpySE526I | ACGT | 4 cut(s) | 352, 607, 945, 1137 |
| HspAI | GCGC | 2 cut(s) | 388, 1304 |
| KpnI | GGTACC | 2 cut(s) | 1328, 1582 |
| Ksp22I | TGATCA | 1 cut(s) | 898 |
| KspAI | GTTAAC | 1 cut(s) | 949 |
| Kzo9I | GATC | 3 cut(s) | 80, 898, 1237 |
| LmnI | GCTCC | 2 cut(s) | 1108, 1267 |
| Lsp1109I | GCAGC | 2 cut(s) | 123, 1160 |
| LweI | GCATC | 3 cut(s) | 307, 445, 1491 |
| MaeI | CTAG | 3 cut(s) | 870, 927, 1268 |
| MaeII | ACGT | 4 cut(s) | 352, 607, 945, 1137 |
| MaeIII | GTNAC | 4 cut(s) | 154, 162, 348, 1152 |
| MalI | GATC | 3 cut(s) | 82, 900, 1239 |
| MboI | GATC | 3 cut(s) | 80, 898, 1237 |
| MflI | RGATCY | 1 cut(s) | 80 |
| MhlI | GDGCHC | 4 cut(s) | 543, 1006, 1264, 1684 |
| MlyI | GAGTC | 4 cut(s) | 178, 526, 1037, 1436 |
| MmeI | TCCRAC | 4 cut(s) | 420, 432, 544, 1358 |
| MroXI | GAANNNNTTC | 1 cut(s) | 883 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1223 |
| MspCI | CTTAAG | 2 cut(s) | 131, 1811 |
| MspI | CCGG | 1 cut(s) | 1882 |
| MspR9I | CCNGG | 2 cut(s) | 1158, 1719 |
| MvaI | CCWGG | 2 cut(s) | 1158, 1719 |
| MvnI | CGCG | 1 cut(s) | 1304 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 142, 145 |
| NdeII | GATC | 3 cut(s) | 80, 898, 1237 |
| NlaIV | GGNNCC | 7 cut(s) | 291, 1004, 1110, 1326, 1476, 1580, 1682 |
| NmuCI | GTSAC | 4 cut(s) | 154, 162, 348, 1152 |
| NspI | RCATGY | 4 cut(s) | 152, 827, 866, 1289 |
| NspV | TTCGAA | 1 cut(s) | 89 |
| PaeI | GCATGC | 1 cut(s) | 152 |
| PciI | ACATGT | 1 cut(s) | 862 |
| PdmI | GAANNNNTTC | 1 cut(s) | 883 |
| PfeI | GAWTC | 3 cut(s) | 116, 577, 1490 |
| PflFI | GACNNNGTC | 3 cut(s) | 40, 160, 1150 |
| PkrI | GCNGC | 4 cut(s) | 138, 147, 1113, 1175 |
| PleI | GAGTC | 4 cut(s) | 178, 526, 1037, 1436 |
| PmaCI | CACGTG | 1 cut(s) | 353 |
| PmlI | CACGTG | 1 cut(s) | 353 |
| PpsI | GAGTC | 4 cut(s) | 178, 526, 1037, 1436 |
| Ppu21I | YACGTR | 2 cut(s) | 353, 1138 |
| PscI | ACATGT | 1 cut(s) | 862 |
| PsiI | TTATAA | 1 cut(s) | 1605 |
| Psp6I | CCWGG | 2 cut(s) | 1156, 1717 |
| PspCI | CACGTG | 1 cut(s) | 353 |
| PspGI | CCWGG | 2 cut(s) | 1156, 1717 |
| PspN4I | GGNNCC | 7 cut(s) | 291, 1004, 1110, 1326, 1476, 1580, 1682 |
| PspOMI | GGGCCC | 2 cut(s) | 1002, 1680 |
| PspPI | GGNCC | 5 cut(s) | 1002, 1003, 1680, 1681, 1879 |
| PsuI | RGATCY | 1 cut(s) | 80 |
| PsyI | GACNNNGTC | 3 cut(s) | 40, 160, 1150 |
| RsaI | GTAC | 4 cut(s) | 1092, 1326, 1571, 1580 |
| RsaNI | GTAC | 4 cut(s) | 1091, 1325, 1570, 1579 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1223 |
| SalI | GTCGAC | 2 cut(s) | 1040, 1424 |
| SatI | GCNGC | 4 cut(s) | 137, 146, 1112, 1174 |
| Sau3AI | GATC | 3 cut(s) | 80, 898, 1237 |
| Sau96I | GGNCC | 5 cut(s) | 1002, 1003, 1680, 1681, 1879 |
| SchI | GAGTC | 4 cut(s) | 178, 526, 1037, 1436 |
| ScrFI | CCNGG | 2 cut(s) | 1158, 1719 |
| SduI | GDGCHC | 4 cut(s) | 543, 1006, 1264, 1684 |
| SfaNI | GCATC | 3 cut(s) | 307, 445, 1491 |
| SfuI | TTCGAA | 1 cut(s) | 89 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1223 |
| SmlI | CTYRAG | 4 cut(s) | 131, 831, 909, 1811 |
| SmoI | CTYRAG | 4 cut(s) | 131, 831, 909, 1811 |
| SphI | GCATGC | 1 cut(s) | 152 |
| SsiI | CCGC | 5 cut(s) | 145, 421, 1112, 1302, 1910 |
| SspI | AATATT | 1 cut(s) | 1204 |
| SspMI | CTAG | 3 cut(s) | 870, 927, 1268 |
| StyD4I | CCNGG | 2 cut(s) | 1156, 1717 |
| TaaI | ACNGT | 6 cut(s) | 417, 634, 953, 1222, 1357, 1675 |
| TaiI | ACGT | 4 cut(s) | 355, 610, 948, 1140 |
| TaqI | TCGA | 5 cut(s) | 89, 217, 1013, 1041, 1425 |
| TauI | GCSGC | 2 cut(s) | 148, 1114 |
| TfiI | GAWTC | 3 cut(s) | 116, 577, 1490 |
| TscAI | CASTG | 2 cut(s) | 159, 1225 |
| TseFI | GTSAC | 4 cut(s) | 154, 162, 348, 1152 |
| TseI | GCWGC | 2 cut(s) | 136, 1173 |
| Tsp45I | GTSAC | 4 cut(s) | 154, 162, 348, 1152 |
| TspDTI | ATGAA | 8 cut(s) | 17, 590, 1244, 1359, 1449, 1720, 1809, 1878 |
| TspRI | CASTG | 2 cut(s) | 159, 1225 |
| Tth111I | GACNNNGTC | 3 cut(s) | 40, 160, 1150 |
| Vha464I | CTTAAG | 2 cut(s) | 131, 1811 |
| XapI | RAATTY | 1 cut(s) | 1903 |
| XceI | RCATGY | 4 cut(s) | 152, 827, 866, 1289 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1253 |
| XmiI | GTMKAC | 2 cut(s) | 1041, 1425 |
| XmnI | GAANNNNTTC | 1 cut(s) | 883 |
| XspI | CTAG | 3 cut(s) | 870, 927, 1268 |