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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 1376 |
| AatII | GACGTC | 1 cut(s) | 1829 |
| Acc36I | ACCTGC | 2 cut(s) | 878, 1376 |
| AccB1I | GGYRCC | 1 cut(s) | 1834 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 723, 1697 |
| AciI | CCGC | 3 cut(s) | 19, 274, 440 |
| AclWI | GGATC | 6 cut(s) | 114, 1451, 1464, 1595, 1681, 1972 |
| AcoI | YGGCCR | 2 cut(s) | 16, 1180 |
| AcsI | RAATTY | 5 cut(s) | 320, 612, 1103, 1233, 1886 |
| AcuI | CTGAAG | 5 cut(s) | 680, 705, 1098, 1269, 1947 |
| AcyI | GRCGYC | 1 cut(s) | 1826 |
| AfaI | GTAC | 8 cut(s) | 393, 835, 1156, 1359, 1440, 1612, 1903, 2024 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 629, 723, 1471, 1697 |
| AflII | CTTAAG | 1 cut(s) | 1115 |
| AjiI | CACGTC | 1 cut(s) | 76 |
| AjnI | CCWGG | 3 cut(s) | 133, 361, 1696 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 917, 949 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1956, 1988 |
| AluBI | AGCT | 7 cut(s) | 801, 1088, 1191, 1247, 1526, 1821, 1953 |
| AluI | AGCT | 7 cut(s) | 801, 1088, 1191, 1247, 1526, 1821, 1953 |
| Alw21I | GWGCWC | 2 cut(s) | 538, 1627 |
| Alw26I | GTCTC | 1 cut(s) | 773 |
| AlwI | GGATC | 6 cut(s) | 114, 1451, 1464, 1595, 1681, 1972 |
| AoxI | GGCC | 3 cut(s) | 16, 714, 1180 |
| ApeKI | GCWGC | 3 cut(s) | 91, 1394, 1664 |
| ApoI | RAATTY | 5 cut(s) | 320, 612, 1103, 1233, 1886 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1192 |
| AspLEI | GCGC | 1 cut(s) | 82 |
| AspS9I | GGNCC | 1 cut(s) | 714 |
| AsuHPI | GGTGA | 5 cut(s) | 296, 836, 1360, 1607, 1889 |
| AsuII | TTCGAA | 1 cut(s) | 1242 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 488, 521 |
| BalI | TGGCCA | 1 cut(s) | 1182 |
| BamHI | GGATCC | 1 cut(s) | 1456 |
| BanI | GGYRCC | 1 cut(s) | 1834 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 653, 685 |
| BbsI | GAAGAC | 1 cut(s) | 1806 |
| Bbv12I | GWGCWC | 2 cut(s) | 538, 1627 |
| BbvI | GCAGC | 3 cut(s) | 103, 1406, 1651 |
| BccI | CCATC | 6 cut(s) | 97, 648, 743, 815, 1139, 1913 |
| BceAI | ACGGC | 1 cut(s) | 798 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 872, 906, 1196, 1230 |
| BciT130I | CCWGG | 3 cut(s) | 135, 363, 1698 |
| BcoDI | GTCTC | 1 cut(s) | 773 |
| BfaI | CTAG | 4 cut(s) | 539, 965, 1001, 1608 |
| BfmI | CTRYAG | 2 cut(s) | 589, 1665 |
| BfoI | RGCGCY | 1 cut(s) | 83 |
| BfrI | CTTAAG | 1 cut(s) | 1115 |
| BfuAI | ACCTGC | 2 cut(s) | 878, 1376 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 77 |
| BisI | GCNGC | 5 cut(s) | 19, 92, 441, 1395, 1665 |
| BlsI | GCNGC | 5 cut(s) | 20, 93, 442, 1396, 1666 |
| Bme1390I | CCNGG | 3 cut(s) | 135, 363, 1698 |
| BmgBI | CACGTC | 1 cut(s) | 76 |
| BmgT120I | GGNCC | 1 cut(s) | 714 |
| BmiI | GGNNCC | 5 cut(s) | 1336, 1458, 1555, 1836, 1923 |
| BmrFI | CCNGG | 3 cut(s) | 135, 363, 1698 |
| BmrI | ACTGGG | 1 cut(s) | 1640 |
| BmsI | GCATC | 6 cut(s) | 78, 331, 427, 1381, 1411, 1851 |
| BmuI | ACTGGG | 1 cut(s) | 1640 |
| BpiI | GAAGAC | 1 cut(s) | 1806 |
| Bpu10I | CCTNAGC | 2 cut(s) | 862, 879 |
| Bpu14I | TTCGAA | 1 cut(s) | 1242 |
| Bsa29I | ATCGAT | 1 cut(s) | 708 |
| BsaHI | GRCGYC | 1 cut(s) | 1826 |
| BsaJI | CCNNGG | 5 cut(s) | 133, 361, 744, 1183, 1697 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 629, 723, 1471, 1697 |
| Bse1I | ACTGG | 4 cut(s) | 458, 490, 1635, 2008 |
| Bse3DI | GCAATG | 3 cut(s) | 1397, 1496, 1642 |
| BseBI | CCWGG | 3 cut(s) | 135, 363, 1698 |
| BseCI | ATCGAT | 1 cut(s) | 708 |
| BseDI | CCNNGG | 5 cut(s) | 133, 361, 744, 1183, 1697 |
| BseGI | GGATG | 7 cut(s) | 298, 346, 700, 754, 1360, 1402, 1420 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 629, 723, 1471, 1697 |
| BseMI | GCAATG | 3 cut(s) | 1397, 1496, 1642 |
| BseMII | CTCAG | 3 cut(s) | 821, 870, 1518 |
| BseNI | ACTGG | 4 cut(s) | 458, 490, 1635, 2008 |
| BseRI | GAGGAG | 5 cut(s) | 311, 1022, 1067, 1484, 1688 |
| BseXI | GCAGC | 3 cut(s) | 103, 1406, 1651 |
| BsgI | GTGCAG | 1 cut(s) | 1010 |
| BshFI | GGCC | 3 cut(s) | 18, 716, 1182 |
| BshNI | GGYRCC | 1 cut(s) | 1834 |
| BshVI | ATCGAT | 1 cut(s) | 708 |
| BsiHKAI | GWGCWC | 2 cut(s) | 538, 1627 |
| BslFI | GGGAC | 2 cut(s) | 606, 886 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 629, 723, 1471, 1697 |
| BsmAI | GTCTC | 1 cut(s) | 773 |
| BsmBI | CGTCTC | 1 cut(s) | 773 |
| BsmFI | GGGAC | 2 cut(s) | 606, 886 |
| BsmI | GAATGC | 2 cut(s) | 900, 1684 |
| BsnI | GGCC | 3 cut(s) | 18, 716, 1182 |
| Bsp119I | TTCGAA | 1 cut(s) | 1242 |
| Bsp1286I | GDGCHC | 2 cut(s) | 538, 1627 |
| Bsp143I | GATC | 7 cut(s) | 55, 106, 185, 1456, 1600, 1686, 1964 |
| BspACI | CCGC | 3 cut(s) | 19, 274, 440 |
| BspANI | GGCC | 3 cut(s) | 18, 716, 1182 |
| BspCNI | CTCAG | 3 cut(s) | 822, 871, 1519 |
| BspDI | ATCGAT | 1 cut(s) | 708 |
| BspLI | GGNNCC | 5 cut(s) | 1336, 1458, 1555, 1836, 1923 |
| BspMAI | CTGCAG | 1 cut(s) | 1669 |
| BspMI | ACCTGC | 2 cut(s) | 878, 1376 |
| BspPI | GGATC | 6 cut(s) | 114, 1451, 1464, 1595, 1681, 1972 |
| BspQI | GCTCTTC | 1 cut(s) | 1074 |
| BspT104I | TTCGAA | 1 cut(s) | 1242 |
| BspT107I | GGYRCC | 1 cut(s) | 1834 |
| BspTI | CTTAAG | 1 cut(s) | 1115 |
| BsrDI | GCAATG | 3 cut(s) | 1397, 1496, 1642 |
| BsrI | ACTGG | 4 cut(s) | 458, 490, 1635, 2008 |
| BssECI | CCNNGG | 5 cut(s) | 133, 361, 744, 1183, 1697 |
| BssMI | GATC | 7 cut(s) | 55, 106, 185, 1456, 1600, 1686, 1964 |
| BssNI | GRCGYC | 1 cut(s) | 1826 |
| BssT1I | CCWWGG | 2 cut(s) | 744, 1183 |
| Bst2UI | CCWGG | 3 cut(s) | 135, 363, 1698 |
| Bst4CI | ACNGT | 8 cut(s) | 581, 600, 976, 1154, 1159, 1585, 1615, 1720 |
| Bst6I | CTCTTC | 2 cut(s) | 1074, 1766 |
| BstACI | GRCGYC | 1 cut(s) | 1826 |
| BstAFI | CTTAAG | 1 cut(s) | 1115 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1106 |
| BstBI | TTCGAA | 1 cut(s) | 1242 |
| BstC8I | GCNNGC | 8 cut(s) | 23, 568, 681, 799, 885, 1086, 1728, 1987 |
| BstDEI | CTNAG | 4 cut(s) | 830, 862, 879, 1527 |
| BstF5I | GGATG | 7 cut(s) | 298, 346, 700, 754, 1360, 1402, 1420 |
| BstH2I | RGCGCY | 1 cut(s) | 83 |
| BstHHI | GCGC | 1 cut(s) | 82 |
| BstKTI | GATC | 7 cut(s) | 58, 109, 188, 1459, 1603, 1689, 1967 |
| BstMAI | GTCTC | 1 cut(s) | 773 |
| BstMBI | GATC | 7 cut(s) | 55, 106, 185, 1456, 1600, 1686, 1964 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 77, 88, 1106, 1188 |
| BstNI | CCWGG | 3 cut(s) | 135, 363, 1698 |
| BstNSI | RCATGY | 1 cut(s) | 887 |
| BstSCI | CCNGG | 3 cut(s) | 133, 361, 1696 |
| BstSFI | CTRYAG | 2 cut(s) | 589, 1665 |
| BstV1I | GCAGC | 3 cut(s) | 103, 1406, 1651 |
| BstV2I | GAAGAC | 1 cut(s) | 1806 |
| BstX2I | RGATCY | 2 cut(s) | 1456, 1686 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 1345, 1588 |
| BstYI | RGATCY | 2 cut(s) | 1456, 1686 |
| Bsu15I | ATCGAT | 1 cut(s) | 708 |
| BsuRI | GGCC | 3 cut(s) | 18, 716, 1182 |
| BsuTUI | ATCGAT | 1 cut(s) | 708 |
| BtgZI | GCGATG | 1 cut(s) | 282 |
| BtrI | CACGTC | 1 cut(s) | 76 |
| BtsCI | GGATG | 7 cut(s) | 298, 346, 700, 754, 1360, 1402, 1420 |
| BtsIMutI | CAGTG | 3 cut(s) | 513, 1581, 2001 |
| BveI | ACCTGC | 2 cut(s) | 878, 1376 |
| Cac8I | GCNNGC | 8 cut(s) | 23, 568, 681, 799, 885, 1086, 1728, 1987 |
| CfoI | GCGC | 1 cut(s) | 82 |
| Cfr13I | GGNCC | 1 cut(s) | 714 |
| ClaI | ATCGAT | 1 cut(s) | 708 |
| Csp6I | GTAC | 8 cut(s) | 392, 834, 1155, 1358, 1439, 1611, 1902, 2023 |
| CviQI | GTAC | 8 cut(s) | 392, 834, 1155, 1358, 1439, 1611, 1902, 2023 |
| DdeI | CTNAG | 4 cut(s) | 830, 862, 879, 1527 |
| DpnI | GATC | 7 cut(s) | 57, 108, 187, 1458, 1602, 1688, 1966 |
| DpnII | GATC | 7 cut(s) | 55, 106, 185, 1456, 1600, 1686, 1964 |
| EaeI | YGGCCR | 2 cut(s) | 16, 1180 |
| Eam1104I | CTCTTC | 2 cut(s) | 1074, 1766 |
| EarI | CTCTTC | 2 cut(s) | 1074, 1766 |
| Eco130I | CCWWGG | 2 cut(s) | 744, 1183 |
| Eco57I | CTGAAG | 5 cut(s) | 680, 705, 1098, 1269, 1947 |
| EcoRI | GAATTC | 1 cut(s) | 320 |
| EcoRII | CCWGG | 3 cut(s) | 133, 361, 1696 |
| EcoT14I | CCWWGG | 2 cut(s) | 744, 1183 |
| ErhI | CCWWGG | 2 cut(s) | 744, 1183 |
| Esp3I | CGTCTC | 1 cut(s) | 773 |
| FaqI | GGGAC | 2 cut(s) | 606, 886 |
| Fnu4HI | GCNGC | 5 cut(s) | 19, 92, 441, 1395, 1665 |
| FokI | GGATG | 7 cut(s) | 305, 353, 707, 761, 1347, 1389, 1427 |
| Fsp4HI | GCNGC | 5 cut(s) | 19, 92, 441, 1395, 1665 |
| FspBI | CTAG | 4 cut(s) | 539, 965, 1001, 1608 |
| GlaI | GCGC | 1 cut(s) | 81 |
| GluI | GCNGC | 5 cut(s) | 19, 92, 441, 1395, 1665 |
| HaeII | RGCGCY | 1 cut(s) | 83 |
| HaeIII | GGCC | 3 cut(s) | 18, 716, 1182 |
| HhaI | GCGC | 1 cut(s) | 82 |
| Hin1I | GRCGYC | 1 cut(s) | 1826 |
| Hin6I | GCGC | 1 cut(s) | 80 |
| HinP1I | GCGC | 1 cut(s) | 80 |
| HindIII | AAGCTT | 1 cut(s) | 799 |
| HinfI | GANTC | 7 cut(s) | 176, 218, 227, 874, 1408, 1802, 1850 |
| HphI | GGTGA | 5 cut(s) | 296, 836, 1360, 1607, 1889 |
| Hpy166II | GTNNAC | 3 cut(s) | 827, 1196, 2023 |
| Hpy188I | TCNGA | 6 cut(s) | 190, 451, 472, 731, 1407, 1969 |
| Hpy188III | TCNNGA | 7 cut(s) | 59, 639, 760, 856, 878, 1412, 1460 |
| Hpy8I | GTNNAC | 3 cut(s) | 827, 1196, 2023 |
| Hpy99I | CGWCG | 1 cut(s) | 77 |
| HpyAV | CCTTC | 8 cut(s) | 275, 517, 1247, 1249, 1265, 1324, 1348, 1614 |
| HpyCH4III | ACNGT | 8 cut(s) | 581, 600, 976, 1154, 1159, 1585, 1615, 1720 |
| HpyCH4IV | ACGT | 3 cut(s) | 75, 503, 1826 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 77, 88, 1106, 1188 |
| HpyF3I | CTNAG | 4 cut(s) | 830, 862, 879, 1527 |
| HpySE526I | ACGT | 3 cut(s) | 75, 503, 1826 |
| Hsp92I | GRCGYC | 1 cut(s) | 1826 |
| HspAI | GCGC | 1 cut(s) | 80 |
| Kzo9I | GATC | 7 cut(s) | 55, 106, 185, 1456, 1600, 1686, 1964 |
| LguI | GCTCTTC | 1 cut(s) | 1074 |
| Lsp1109I | GCAGC | 3 cut(s) | 103, 1406, 1651 |
| LweI | GCATC | 6 cut(s) | 78, 331, 427, 1381, 1411, 1851 |
| MaeI | CTAG | 4 cut(s) | 539, 965, 1001, 1608 |
| MaeII | ACGT | 3 cut(s) | 75, 503, 1826 |
| MaeIII | GTNAC | 3 cut(s) | 668, 911, 1999 |
| MalI | GATC | 7 cut(s) | 57, 108, 187, 1458, 1602, 1688, 1966 |
| MboI | GATC | 7 cut(s) | 55, 106, 185, 1456, 1600, 1686, 1964 |
| MfeI | CAATTG | 1 cut(s) | 1443 |
| MflI | RGATCY | 2 cut(s) | 1456, 1686 |
| MhlI | GDGCHC | 2 cut(s) | 538, 1627 |
| MlsI | TGGCCA | 1 cut(s) | 1182 |
| MluNI | TGGCCA | 1 cut(s) | 1182 |
| MlyI | GAGTC | 4 cut(s) | 185, 227, 868, 1796 |
| MmeI | TCCRAC | 3 cut(s) | 258, 1362, 1721 |
| Mox20I | TGGCCA | 1 cut(s) | 1182 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1192 |
| MscI | TGGCCA | 1 cut(s) | 1182 |
| MseI | TTAA | 3 cut(s) | 240, 1116, 1722 |
| MslI | CAYNNNNRTG | 4 cut(s) | 383, 1170, 1586, 2018 |
| Msp20I | TGGCCA | 1 cut(s) | 1182 |
| MspA1I | CMGCKG | 1 cut(s) | 21 |
| MspCI | CTTAAG | 1 cut(s) | 1115 |
| MspR9I | CCNGG | 3 cut(s) | 135, 363, 1698 |
| MunI | CAATTG | 1 cut(s) | 1443 |
| Mva1269I | GAATGC | 2 cut(s) | 900, 1684 |
| MvaI | CCWGG | 3 cut(s) | 135, 363, 1698 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 77, 88, 1106, 1188 |
| NdeII | GATC | 7 cut(s) | 55, 106, 185, 1456, 1600, 1686, 1964 |
| NlaIV | GGNNCC | 5 cut(s) | 1336, 1458, 1555, 1836, 1923 |
| NmuCI | GTSAC | 1 cut(s) | 1999 |
| NspI | RCATGY | 1 cut(s) | 887 |
| NspV | TTCGAA | 1 cut(s) | 1242 |
| PaeI | GCATGC | 1 cut(s) | 887 |
| PaqCI | CACCTGC | 1 cut(s) | 1376 |
| PciSI | GCTCTTC | 1 cut(s) | 1074 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 622 |
| PctI | GAATGC | 2 cut(s) | 900, 1684 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1192 |
| PfeI | GAWTC | 3 cut(s) | 227, 1408, 1850 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 723, 1697 |
| PkrI | GCNGC | 5 cut(s) | 20, 93, 442, 1396, 1666 |
| PleI | GAGTC | 4 cut(s) | 184, 226, 868, 1796 |
| PpsI | GAGTC | 4 cut(s) | 184, 226, 868, 1796 |
| Psp6I | CCWGG | 3 cut(s) | 133, 361, 1696 |
| PspGI | CCWGG | 3 cut(s) | 133, 361, 1696 |
| PspN4I | GGNNCC | 5 cut(s) | 1336, 1458, 1555, 1836, 1923 |
| PspPI | GGNCC | 1 cut(s) | 714 |
| PstI | CTGCAG | 1 cut(s) | 1669 |
| PsuI | RGATCY | 2 cut(s) | 1456, 1686 |
| RsaI | GTAC | 8 cut(s) | 393, 835, 1156, 1359, 1440, 1612, 1903, 2024 |
| RsaNI | GTAC | 8 cut(s) | 392, 834, 1155, 1358, 1439, 1611, 1902, 2023 |
| RseI | CAYNNNNRTG | 4 cut(s) | 383, 1170, 1586, 2018 |
| SapI | GCTCTTC | 1 cut(s) | 1074 |
| SaqAI | TTAA | 3 cut(s) | 240, 1116, 1722 |
| SatI | GCNGC | 5 cut(s) | 19, 92, 441, 1395, 1665 |
| Sau3AI | GATC | 7 cut(s) | 55, 106, 185, 1456, 1600, 1686, 1964 |
| Sau96I | GGNCC | 1 cut(s) | 714 |
| SchI | GAGTC | 4 cut(s) | 185, 227, 868, 1796 |
| ScrFI | CCNGG | 3 cut(s) | 135, 363, 1698 |
| SduI | GDGCHC | 2 cut(s) | 538, 1627 |
| SfaNI | GCATC | 6 cut(s) | 78, 331, 427, 1381, 1411, 1851 |
| SfcI | CTRYAG | 2 cut(s) | 589, 1665 |
| SfuI | TTCGAA | 1 cut(s) | 1242 |
| SmiMI | CAYNNNNRTG | 4 cut(s) | 383, 1170, 1586, 2018 |
| SmlI | CTYRAG | 1 cut(s) | 1115 |
| SmoI | CTYRAG | 1 cut(s) | 1115 |
| SphI | GCATGC | 1 cut(s) | 887 |
| SsiI | CCGC | 3 cut(s) | 19, 274, 440 |
| SspMI | CTAG | 4 cut(s) | 539, 965, 1001, 1608 |
| StyD4I | CCNGG | 3 cut(s) | 133, 361, 1696 |
| StyI | CCWWGG | 2 cut(s) | 744, 1183 |
| TaaI | ACNGT | 8 cut(s) | 581, 600, 976, 1154, 1159, 1585, 1615, 1720 |
| TaiI | ACGT | 3 cut(s) | 78, 506, 1829 |
| TaqI | TCGA | 6 cut(s) | 54, 616, 708, 892, 1242, 1805 |
| TatI | WGTACW | 3 cut(s) | 391, 833, 1610 |
| TauI | GCSGC | 2 cut(s) | 21, 443 |
| TfiI | GAWTC | 3 cut(s) | 227, 1408, 1850 |
| Tru1I | TTAA | 3 cut(s) | 240, 1116, 1722 |
| Tru9I | TTAA | 3 cut(s) | 240, 1116, 1722 |
| TscAI | CASTG | 3 cut(s) | 513, 1588, 2008 |
| TseFI | GTSAC | 1 cut(s) | 1999 |
| TseI | GCWGC | 3 cut(s) | 91, 1394, 1664 |
| Tsp45I | GTSAC | 1 cut(s) | 1999 |
| TspDTI | ATGAA | 9 cut(s) | 333, 401, 624, 1274, 1518, 1607, 1722, 1842, 1945 |
| TspGWI | ACGGA | 1 cut(s) | 964 |
| TspRI | CASTG | 3 cut(s) | 513, 1588, 2008 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 723, 1697 |
| Vha464I | CTTAAG | 1 cut(s) | 1115 |
| XapI | RAATTY | 5 cut(s) | 320, 612, 1103, 1233, 1886 |
| XceI | RCATGY | 1 cut(s) | 887 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1192 |
| XspI | CTAG | 4 cut(s) | 539, 965, 1001, 1608 |
| ZraI | GACGTC | 1 cut(s) | 1827 |