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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 685 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 695, 1596 |
| AccI | GTMKAC | 1 cut(s) | 1314 |
| AccIII | TCCGGA | 2 cut(s) | 157, 1618 |
| AciI | CCGC | 2 cut(s) | 506, 1120 |
| AclWI | GGATC | 3 cut(s) | 1484, 1497, 1661 |
| AcoI | YGGCCR | 2 cut(s) | 735, 947 |
| AcsI | RAATTY | 5 cut(s) | 838, 1005, 1227, 1407, 1690 |
| AcuI | CTGAAG | 4 cut(s) | 120, 290, 894, 1488 |
| AdeI | CACNNNGTG | 1 cut(s) | 980 |
| AfaI | GTAC | 3 cut(s) | 630, 678, 693 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 120, 695, 977, 1596 |
| AgsI | TTSAA | 9 cut(s) | 40, 77, 217, 248, 533, 596, 1268, 1279, 1595 |
| AjnI | CCWGG | 1 cut(s) | 976 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 545, 577 |
| AleI | CACNNNNGTG | 1 cut(s) | 1329 |
| Alw21I | GWGCWC | 2 cut(s) | 138, 1366 |
| Alw26I | GTCTC | 3 cut(s) | 713, 788, 853 |
| AlwI | GGATC | 3 cut(s) | 1484, 1497, 1661 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 863 |
| Aor13HI | TCCGGA | 2 cut(s) | 157, 1618 |
| AoxI | GGCC | 3 cut(s) | 735, 947, 1016 |
| ApeKI | GCWGC | 7 cut(s) | 418, 503, 515, 610, 883, 1236, 1239 |
| ApoI | RAATTY | 5 cut(s) | 838, 1005, 1227, 1407, 1690 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1476 |
| AspLEI | GCGC | 1 cut(s) | 1662 |
| AspS9I | GGNCC | 2 cut(s) | 1151, 1417 |
| AsuHPI | GGTGA | 4 cut(s) | 553, 710, 728, 1529 |
| AvaII | GGWCC | 2 cut(s) | 1151, 1417 |
| AxyI | CCTNAGG | 1 cut(s) | 48 |
| BalI | TGGCCA | 1 cut(s) | 737 |
| BamHI | GGATCC | 1 cut(s) | 1489 |
| BanI | GGYRCC | 1 cut(s) | 685 |
| BanII | GRGCYC | 1 cut(s) | 1366 |
| BbsI | GAAGAC | 1 cut(s) | 1053 |
| Bbv12I | GWGCWC | 2 cut(s) | 138, 1366 |
| BbvCI | CCTCAGC | 2 cut(s) | 354, 537 |
| BbvI | GCAGC | 7 cut(s) | 405, 490, 502, 622, 870, 1226, 1248 |
| BccI | CCATC | 3 cut(s) | 401, 1072, 1142 |
| BceAI | ACGGC | 1 cut(s) | 245 |
| BciT130I | CCWGG | 1 cut(s) | 978 |
| BciVI | GTATCC | 1 cut(s) | 334 |
| BclI | TGATCA | 1 cut(s) | 1753 |
| BcoDI | GTCTC | 3 cut(s) | 713, 788, 853 |
| BfaI | CTAG | 4 cut(s) | 258, 281, 392, 680 |
| BfuI | GTATCC | 1 cut(s) | 334 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 229 |
| BisI | GCNGC | 7 cut(s) | 419, 504, 516, 611, 884, 1237, 1240 |
| BlsI | GCNGC | 7 cut(s) | 420, 505, 517, 612, 885, 1238, 1241 |
| Bme1390I | CCNGG | 1 cut(s) | 978 |
| Bme18I | GGWCC | 2 cut(s) | 1151, 1417 |
| BmgT120I | GGNCC | 2 cut(s) | 1151, 1417 |
| BmiI | GGNNCC | 4 cut(s) | 687, 956, 1419, 1491 |
| BmrFI | CCNGG | 1 cut(s) | 978 |
| BmrI | ACTGGG | 1 cut(s) | 609 |
| BmsI | GCATC | 2 cut(s) | 594, 913 |
| BmuI | ACTGGG | 1 cut(s) | 609 |
| BoxI | GACNNNNGTC | 1 cut(s) | 938 |
| BpiI | GAAGAC | 1 cut(s) | 1053 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 469, 501 |
| Bpu10I | CCTNAGC | 3 cut(s) | 309, 354, 537 |
| BpuEI | CTTGAG | 1 cut(s) | 812 |
| BsaI | GGTCTC | 2 cut(s) | 713, 788 |
| BsaJI | CCNNGG | 3 cut(s) | 490, 814, 950 |
| BsaWI | WCCGGW | 2 cut(s) | 157, 1618 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 120, 695, 977, 1596 |
| Bse1I | ACTGG | 3 cut(s) | 615, 689, 738 |
| Bse21I | CCTNAGG | 1 cut(s) | 48 |
| Bse3DI | GCAATG | 1 cut(s) | 199 |
| BseAI | TCCGGA | 2 cut(s) | 157, 1618 |
| BseBI | CCWGG | 1 cut(s) | 978 |
| BseDI | CCNNGG | 3 cut(s) | 490, 814, 950 |
| BseGI | GGATG | 3 cut(s) | 928, 1134, 1168 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 120, 695, 977, 1596 |
| BseMI | GCAATG | 1 cut(s) | 199 |
| BseMII | CTCAG | 4 cut(s) | 39, 345, 366, 528 |
| BseNI | ACTGG | 3 cut(s) | 615, 689, 738 |
| BseRI | GAGGAG | 7 cut(s) | 299, 302, 344, 347, 371, 668, 848 |
| BseXI | GCAGC | 7 cut(s) | 405, 490, 502, 622, 870, 1226, 1248 |
| BsgI | GTGCAG | 4 cut(s) | 501, 629, 957, 1062 |
| BshFI | GGCC | 3 cut(s) | 737, 949, 1018 |
| BshNI | GGYRCC | 1 cut(s) | 685 |
| BsiHKAI | GWGCWC | 2 cut(s) | 138, 1366 |
| BsiSI | CCGG | 2 cut(s) | 158, 1619 |
| BslFI | GGGAC | 3 cut(s) | 415, 946, 1403 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 120, 695, 977, 1596 |
| BsmAI | GTCTC | 3 cut(s) | 713, 788, 853 |
| BsmFI | GGGAC | 3 cut(s) | 415, 946, 1403 |
| BsmI | GAATGC | 1 cut(s) | 1614 |
| BsnI | GGCC | 3 cut(s) | 737, 949, 1018 |
| Bso31I | GGTCTC | 2 cut(s) | 713, 788 |
| Bsp1286I | GDGCHC | 2 cut(s) | 138, 1366 |
| Bsp13I | TCCGGA | 2 cut(s) | 157, 1618 |
| Bsp143I | GATC | 3 cut(s) | 1489, 1653, 1753 |
| Bsp19I | CCATGG | 2 cut(s) | 490, 814 |
| BspACI | CCGC | 2 cut(s) | 506, 1120 |
| BspANI | GGCC | 3 cut(s) | 737, 949, 1018 |
| BspCNI | CTCAG | 4 cut(s) | 40, 346, 367, 529 |
| BspEI | TCCGGA | 2 cut(s) | 157, 1618 |
| BspLI | GGNNCC | 4 cut(s) | 687, 956, 1419, 1491 |
| BspPI | GGATC | 3 cut(s) | 1484, 1497, 1661 |
| BspT107I | GGYRCC | 1 cut(s) | 685 |
| BspTNI | GGTCTC | 2 cut(s) | 713, 788 |
| BsrDI | GCAATG | 1 cut(s) | 199 |
| BsrI | ACTGG | 3 cut(s) | 615, 689, 738 |
| BssECI | CCNNGG | 3 cut(s) | 490, 814, 950 |
| BssMI | GATC | 3 cut(s) | 1489, 1653, 1753 |
| BssT1I | CCWWGG | 2 cut(s) | 490, 814 |
| Bst2UI | CCWGG | 1 cut(s) | 978 |
| Bst4CI | ACNGT | 4 cut(s) | 633, 748, 775, 865 |
| Bst6I | CTCTTC | 3 cut(s) | 591, 645, 696 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1060 |
| BstC8I | GCNNGC | 2 cut(s) | 1366, 1612 |
| BstDEI | CTNAG | 7 cut(s) | 48, 309, 354, 375, 537, 1287, 1369 |
| BstDSI | CCRYGG | 2 cut(s) | 490, 814 |
| BstF5I | GGATG | 3 cut(s) | 928, 1134, 1168 |
| BstHHI | GCGC | 1 cut(s) | 1662 |
| BstKTI | GATC | 3 cut(s) | 1492, 1656, 1756 |
| BstMAI | GTCTC | 3 cut(s) | 713, 788, 853 |
| BstMBI | GATC | 3 cut(s) | 1489, 1653, 1753 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 229, 512, 1060, 1772 |
| BstNI | CCWGG | 1 cut(s) | 978 |
| BstNSI | RCATGY | 1 cut(s) | 1784 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 938 |
| BstSCI | CCNGG | 1 cut(s) | 976 |
| BstV1I | GCAGC | 7 cut(s) | 405, 490, 502, 622, 870, 1226, 1248 |
| BstV2I | GAAGAC | 1 cut(s) | 1053 |
| BstX2I | RGATCY | 1 cut(s) | 1489 |
| BstYI | RGATCY | 1 cut(s) | 1489 |
| Bsu36I | CCTNAGG | 1 cut(s) | 48 |
| BsuI | GTATCC | 1 cut(s) | 334 |
| BsuRI | GGCC | 3 cut(s) | 737, 949, 1018 |
| BtgI | CCRYGG | 2 cut(s) | 490, 814 |
| BtgZI | GCGATG | 1 cut(s) | 1495 |
| BtsCI | GGATG | 3 cut(s) | 928, 1134, 1168 |
| BtsIMutI | CAGTG | 2 cut(s) | 622, 1737 |
| Cac8I | GCNNGC | 2 cut(s) | 1366, 1612 |
| CaiI | CAGNNNCTG | 1 cut(s) | 863 |
| CfoI | GCGC | 1 cut(s) | 1662 |
| Cfr13I | GGNCC | 2 cut(s) | 1151, 1417 |
| CsiI | ACCWGGT | 1 cut(s) | 976 |
| Csp6I | GTAC | 3 cut(s) | 629, 677, 692 |
| CviQI | GTAC | 3 cut(s) | 629, 677, 692 |
| DdeI | CTNAG | 7 cut(s) | 48, 309, 354, 375, 537, 1287, 1369 |
| DpnI | GATC | 3 cut(s) | 1491, 1655, 1755 |
| DpnII | GATC | 3 cut(s) | 1489, 1653, 1753 |
| DraI | TTTAAA | 1 cut(s) | 1086 |
| DraIII | CACNNNGTG | 1 cut(s) | 980 |
| EaeI | YGGCCR | 2 cut(s) | 735, 947 |
| Eam1104I | CTCTTC | 3 cut(s) | 591, 645, 696 |
| EarI | CTCTTC | 3 cut(s) | 591, 645, 696 |
| Ecl136II | GAGCTC | 1 cut(s) | 1364 |
| Eco130I | CCWWGG | 2 cut(s) | 490, 814 |
| Eco24I | GRGCYC | 1 cut(s) | 1366 |
| Eco31I | GGTCTC | 2 cut(s) | 713, 788 |
| Eco47I | GGWCC | 2 cut(s) | 1151, 1417 |
| Eco53kI | GAGCTC | 1 cut(s) | 1364 |
| Eco57I | CTGAAG | 4 cut(s) | 120, 290, 894, 1488 |
| Eco81I | CCTNAGG | 1 cut(s) | 48 |
| EcoICRI | GAGCTC | 1 cut(s) | 1364 |
| EcoRII | CCWGG | 1 cut(s) | 976 |
| EcoT14I | CCWWGG | 2 cut(s) | 490, 814 |
| EcoT22I | ATGCAT | 1 cut(s) | 1100 |
| EcoT38I | GRGCYC | 1 cut(s) | 1366 |
| ErhI | CCWWGG | 2 cut(s) | 490, 814 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 1034, 1066 |
| FaqI | GGGAC | 3 cut(s) | 415, 946, 1403 |
| FbaI | TGATCA | 1 cut(s) | 1753 |
| FblI | GTMKAC | 1 cut(s) | 1314 |
| Fnu4HI | GCNGC | 7 cut(s) | 419, 504, 516, 611, 884, 1237, 1240 |
| FokI | GGATG | 3 cut(s) | 935, 1121, 1155 |
| FriOI | GRGCYC | 1 cut(s) | 1366 |
| Fsp4HI | GCNGC | 7 cut(s) | 419, 504, 516, 611, 884, 1237, 1240 |
| FspBI | CTAG | 4 cut(s) | 258, 281, 392, 680 |
| GlaI | GCGC | 1 cut(s) | 1661 |
| GluI | GCNGC | 7 cut(s) | 419, 504, 516, 611, 884, 1237, 1240 |
| HaeIII | GGCC | 3 cut(s) | 737, 949, 1018 |
| HapII | CCGG | 2 cut(s) | 158, 1619 |
| HhaI | GCGC | 1 cut(s) | 1662 |
| Hin6I | GCGC | 1 cut(s) | 1660 |
| HinP1I | GCGC | 1 cut(s) | 1660 |
| HincII | GTYRAC | 1 cut(s) | 1315 |
| HindII | GTYRAC | 1 cut(s) | 1315 |
| HindIII | AAGCTT | 1 cut(s) | 876 |
| HinfI | GANTC | 5 cut(s) | 154, 428, 1069, 1459, 1506 |
| HpaII | CCGG | 2 cut(s) | 158, 1619 |
| HphI | GGTGA | 4 cut(s) | 553, 710, 728, 1529 |
| Hpy166II | GTNNAC | 2 cut(s) | 1315, 1735 |
| Hpy188I | TCNGA | 1 cut(s) | 127 |
| Hpy188III | TCNNGA | 6 cut(s) | 158, 468, 872, 1535, 1619, 1796 |
| Hpy8I | GTNNAC | 2 cut(s) | 1315, 1735 |
| HpyCH4III | ACNGT | 4 cut(s) | 633, 748, 775, 865 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 229, 512, 1060, 1772 |
| HpyF3I | CTNAG | 7 cut(s) | 48, 309, 354, 375, 537, 1287, 1369 |
| HspAI | GCGC | 1 cut(s) | 1660 |
| Kpn2I | TCCGGA | 2 cut(s) | 157, 1618 |
| Ksp22I | TGATCA | 1 cut(s) | 1753 |
| Kzo9I | GATC | 3 cut(s) | 1489, 1653, 1753 |
| LmnI | GCTCC | 3 cut(s) | 160, 415, 1621 |
| Lsp1109I | GCAGC | 7 cut(s) | 405, 490, 502, 622, 870, 1226, 1248 |
| LweI | GCATC | 2 cut(s) | 594, 913 |
| MabI | ACCWGGT | 1 cut(s) | 976 |
| MaeI | CTAG | 4 cut(s) | 258, 281, 392, 680 |
| MaeIII | GTNAC | 1 cut(s) | 442 |
| MalI | GATC | 3 cut(s) | 1491, 1655, 1755 |
| MboI | GATC | 3 cut(s) | 1489, 1653, 1753 |
| MflI | RGATCY | 1 cut(s) | 1489 |
| MhlI | GDGCHC | 2 cut(s) | 138, 1366 |
| MlsI | TGGCCA | 1 cut(s) | 737 |
| MluNI | TGGCCA | 1 cut(s) | 737 |
| MlyI | GAGTC | 2 cut(s) | 437, 1500 |
| MmeI | TCCRAC | 1 cut(s) | 1376 |
| Mox20I | TGGCCA | 1 cut(s) | 737 |
| Mph1103I | ATGCAT | 1 cut(s) | 1100 |
| MroI | TCCGGA | 2 cut(s) | 157, 1618 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1476 |
| MscI | TGGCCA | 1 cut(s) | 737 |
| MseI | TTAA | 8 cut(s) | 9, 83, 453, 569, 635, 1085, 1496, 1548 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1329 |
| Msp20I | TGGCCA | 1 cut(s) | 737 |
| MspA1I | CMGCKG | 2 cut(s) | 1239, 1466 |
| MspI | CCGG | 2 cut(s) | 158, 1619 |
| MspR9I | CCNGG | 1 cut(s) | 978 |
| Mva1269I | GAATGC | 1 cut(s) | 1614 |
| MvaI | CCWGG | 1 cut(s) | 978 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 229, 512, 1060, 1772 |
| NcoI | CCATGG | 2 cut(s) | 490, 814 |
| NdeII | GATC | 3 cut(s) | 1489, 1653, 1753 |
| NlaIV | GGNNCC | 4 cut(s) | 687, 956, 1419, 1491 |
| NmeAIII | GCCGAG | 1 cut(s) | 975 |
| NmuCI | GTSAC | 1 cut(s) | 442 |
| NsiI | ATGCAT | 1 cut(s) | 1100 |
| NspI | RCATGY | 1 cut(s) | 1784 |
| OliI | CACNNNNGTG | 1 cut(s) | 1329 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1311 |
| PctI | GAATGC | 1 cut(s) | 1614 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1476 |
| PfeI | GAWTC | 3 cut(s) | 154, 1069, 1459 |
| PflFI | GACNNNGTC | 2 cut(s) | 1301, 1447 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 695, 1596 |
| PkrI | GCNGC | 7 cut(s) | 420, 505, 517, 612, 885, 1238, 1241 |
| PleI | GAGTC | 2 cut(s) | 436, 1500 |
| PpsI | GAGTC | 2 cut(s) | 436, 1500 |
| PshAI | GACNNNNGTC | 1 cut(s) | 938 |
| Psp124BI | GAGCTC | 1 cut(s) | 1366 |
| Psp6I | CCWGG | 1 cut(s) | 976 |
| PspGI | CCWGG | 1 cut(s) | 976 |
| PspN4I | GGNNCC | 4 cut(s) | 687, 956, 1419, 1491 |
| PspPI | GGNCC | 2 cut(s) | 1151, 1417 |
| PstNI | CAGNNNCTG | 1 cut(s) | 863 |
| PsuI | RGATCY | 1 cut(s) | 1489 |
| PsyI | GACNNNGTC | 2 cut(s) | 1301, 1447 |
| PvuII | CAGCTG | 2 cut(s) | 1239, 1466 |
| RsaI | GTAC | 3 cut(s) | 630, 678, 693 |
| RsaNI | GTAC | 3 cut(s) | 629, 677, 692 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1329 |
| SacI | GAGCTC | 1 cut(s) | 1366 |
| SalI | GTCGAC | 1 cut(s) | 1313 |
| SaqAI | TTAA | 8 cut(s) | 9, 83, 453, 569, 635, 1085, 1496, 1548 |
| SatI | GCNGC | 7 cut(s) | 419, 504, 516, 611, 884, 1237, 1240 |
| Sau3AI | GATC | 3 cut(s) | 1489, 1653, 1753 |
| Sau96I | GGNCC | 2 cut(s) | 1151, 1417 |
| SchI | GAGTC | 2 cut(s) | 437, 1500 |
| ScrFI | CCNGG | 1 cut(s) | 978 |
| SduI | GDGCHC | 2 cut(s) | 138, 1366 |
| SexAI | ACCWGGT | 1 cut(s) | 976 |
| SfaNI | GCATC | 2 cut(s) | 594, 913 |
| SinI | GGWCC | 2 cut(s) | 1151, 1417 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1329 |
| SmlI | CTYRAG | 1 cut(s) | 791 |
| SmoI | CTYRAG | 1 cut(s) | 791 |
| SsiI | CCGC | 2 cut(s) | 506, 1120 |
| SspI | AATATT | 1 cut(s) | 529 |
| SspMI | CTAG | 4 cut(s) | 258, 281, 392, 680 |
| SstI | GAGCTC | 1 cut(s) | 1366 |
| StyD4I | CCNGG | 1 cut(s) | 976 |
| StyI | CCWWGG | 2 cut(s) | 490, 814 |
| TaaI | ACNGT | 4 cut(s) | 633, 748, 775, 865 |
| TaqI | TCGA | 5 cut(s) | 405, 588, 786, 1067, 1314 |
| TatI | WGTACW | 2 cut(s) | 676, 691 |
| TfiI | GAWTC | 3 cut(s) | 154, 1069, 1459 |
| Tru1I | TTAA | 8 cut(s) | 9, 83, 453, 569, 635, 1085, 1496, 1548 |
| Tru9I | TTAA | 8 cut(s) | 9, 83, 453, 569, 635, 1085, 1496, 1548 |
| TscAI | CASTG | 2 cut(s) | 622, 1744 |
| TseFI | GTSAC | 1 cut(s) | 442 |
| TseI | GCWGC | 7 cut(s) | 418, 503, 515, 610, 883, 1236, 1239 |
| Tsp45I | GTSAC | 1 cut(s) | 442 |
| TspDTI | ATGAA | 6 cut(s) | 591, 1271, 1307, 1375, 1474, 1682 |
| TspGWI | ACGGA | 1 cut(s) | 1482 |
| TspRI | CASTG | 2 cut(s) | 622, 1744 |
| Tth111I | GACNNNGTC | 2 cut(s) | 1301, 1447 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 695, 1596 |
| VpaK11BI | GGWCC | 2 cut(s) | 1151, 1417 |
| XapI | RAATTY | 5 cut(s) | 838, 1005, 1227, 1407, 1690 |
| XceI | RCATGY | 1 cut(s) | 1784 |
| XcmI | CCANNNNNNNNNTGG | 4 cut(s) | 730, 745, 1145, 1483 |
| XmiI | GTMKAC | 1 cut(s) | 1314 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1476 |
| XspI | CTAG | 4 cut(s) | 258, 281, 392, 680 |
| Zsp2I | ATGCAT | 1 cut(s) | 1100 |