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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc16I | TGCGCA | 1 cut(s) | 159 |
| Acc65I | GGTACC | 1 cut(s) | 1415 |
| AccB1I | GGYRCC | 2 cut(s) | 145, 1415 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1964 |
| AccI | GTMKAC | 1 cut(s) | 1386 |
| AccII | CGCG | 2 cut(s) | 823, 1411 |
| AciI | CCGC | 1 cut(s) | 1145 |
| AclWI | GGATC | 5 cut(s) | 385, 776, 789, 1931, 1977 |
| AcsI | RAATTY | 1 cut(s) | 530 |
| AcuI | CTGAAG | 1 cut(s) | 1533 |
| AcyI | GRCGYC | 1 cut(s) | 1036 |
| AfaI | GTAC | 6 cut(s) | 314, 611, 1225, 1417, 1422, 1883 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 1675, 1964 |
| AflII | CTTAAG | 1 cut(s) | 62 |
| AflIII | ACRYGT | 2 cut(s) | 206, 2149 |
| AgeI | ACCGGT | 1 cut(s) | 148 |
| AluBI | AGCT | 8 cut(s) | 16, 445, 463, 515, 625, 1382, 1568, 1831 |
| AluI | AGCT | 8 cut(s) | 16, 445, 463, 515, 625, 1382, 1568, 1831 |
| Alw21I | GWGCWC | 1 cut(s) | 2017 |
| Alw26I | GTCTC | 3 cut(s) | 191, 694, 2087 |
| AlwI | GGATC | 5 cut(s) | 385, 776, 789, 1931, 1977 |
| AoxI | GGCC | 4 cut(s) | 858, 1198, 1561, 1589 |
| ApeKI | GCWGC | 5 cut(s) | 16, 344, 512, 1157, 1169 |
| ApoI | RAATTY | 1 cut(s) | 530 |
| AsiGI | ACCGGT | 1 cut(s) | 148 |
| Asp718I | GGTACC | 1 cut(s) | 1415 |
| AspLEI | GCGC | 3 cut(s) | 160, 823, 1411 |
| AspS9I | GGNCC | 1 cut(s) | 1199 |
| AsuHPI | GGTGA | 3 cut(s) | 588, 1782, 2066 |
| AxyI | CCTNAGG | 3 cut(s) | 141, 603, 786 |
| BamHI | GGATCC | 1 cut(s) | 781 |
| BanI | GGYRCC | 2 cut(s) | 145, 1415 |
| BarI | GAAGNNNNNNTAC | 2 cut(s) | 1809, 1841 |
| BbsI | GAAGAC | 3 cut(s) | 18, 582, 642 |
| Bbv12I | GWGCWC | 1 cut(s) | 2017 |
| BbvI | GCAGC | 5 cut(s) | 3, 356, 524, 1169, 1181 |
| BccI | CCATC | 7 cut(s) | 73, 133, 902, 920, 1211, 1576, 2088 |
| BceAI | ACGGC | 1 cut(s) | 1701 |
| BcgI | CGANNNNNNTGC | 4 cut(s) | 96, 130, 337, 371 |
| BciVI | GTATCC | 1 cut(s) | 729 |
| BclI | TGATCA | 2 cut(s) | 2023, 2056 |
| BcoDI | GTCTC | 3 cut(s) | 191, 694, 2087 |
| BfaI | CTAG | 8 cut(s) | 84, 540, 654, 1032, 1260, 1424, 1601, 1629 |
| BfmI | CTRYAG | 3 cut(s) | 510, 1557, 1569 |
| BfrI | CTTAAG | 1 cut(s) | 62 |
| BfuI | GTATCC | 1 cut(s) | 729 |
| BglII | AGATCT | 1 cut(s) | 1126 |
| BisI | GCNGC | 5 cut(s) | 17, 345, 513, 1158, 1170 |
| BlsI | GCNGC | 5 cut(s) | 18, 346, 514, 1159, 1171 |
| BmgT120I | GGNCC | 1 cut(s) | 1199 |
| BmiI | GGNNCC | 4 cut(s) | 147, 783, 1201, 1417 |
| BmsI | GCATC | 5 cut(s) | 274, 345, 415, 1256, 1423 |
| BpiI | GAAGAC | 3 cut(s) | 18, 582, 642 |
| Bsa29I | ATCGAT | 1 cut(s) | 795 |
| BsaBI | GATNNNNATC | 2 cut(s) | 395, 795 |
| BsaHI | GRCGYC | 1 cut(s) | 1036 |
| BsaJI | CCNNGG | 3 cut(s) | 948, 1275, 1958 |
| BsaWI | WCCGGW | 2 cut(s) | 148, 2051 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 987, 1017 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 1675, 1964 |
| Bse118I | RCCGGY | 1 cut(s) | 148 |
| Bse1I | ACTGG | 2 cut(s) | 638, 2084 |
| Bse21I | CCTNAGG | 3 cut(s) | 141, 603, 786 |
| Bse3DI | GCAATG | 1 cut(s) | 1483 |
| Bse8I | GATNNNNATC | 2 cut(s) | 395, 795 |
| BseCI | ATCGAT | 1 cut(s) | 795 |
| BseDI | CCNNGG | 3 cut(s) | 948, 1275, 1958 |
| BseGI | GGATG | 6 cut(s) | 430, 918, 962, 1203, 2070, 2080 |
| BseJI | GATNNNNATC | 2 cut(s) | 395, 795 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 1675, 1964 |
| BseMI | GCAATG | 1 cut(s) | 1483 |
| BseMII | CTCAG | 4 cut(s) | 617, 654, 1242, 1244 |
| BseNI | ACTGG | 2 cut(s) | 638, 2084 |
| BseRI | GAGGAG | 3 cut(s) | 1002, 1109, 1218 |
| BseXI | GCAGC | 5 cut(s) | 3, 356, 524, 1169, 1181 |
| BseYI | CCCAGC | 1 cut(s) | 1238 |
| Bsh1236I | CGCG | 2 cut(s) | 823, 1411 |
| Bsh1285I | CGRYCG | 1 cut(s) | 799 |
| BshFI | GGCC | 4 cut(s) | 860, 1200, 1563, 1591 |
| BshNI | GGYRCC | 2 cut(s) | 145, 1415 |
| BshTI | ACCGGT | 1 cut(s) | 148 |
| BshVI | ATCGAT | 1 cut(s) | 795 |
| BsiEI | CGRYCG | 1 cut(s) | 799 |
| BsiHKAI | GWGCWC | 1 cut(s) | 2017 |
| BsiSI | CCGG | 2 cut(s) | 149, 2052 |
| BslFI | GGGAC | 1 cut(s) | 1981 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 1675, 1964 |
| BsmAI | GTCTC | 3 cut(s) | 191, 694, 2087 |
| BsmBI | CGTCTC | 2 cut(s) | 694, 2087 |
| BsmFI | GGGAC | 1 cut(s) | 1981 |
| BsmI | GAATGC | 3 cut(s) | 491, 1249, 1695 |
| BsnI | GGCC | 4 cut(s) | 860, 1200, 1563, 1591 |
| Bsp1286I | GDGCHC | 1 cut(s) | 2017 |
| Bsp19I | CCATGG | 2 cut(s) | 948, 1958 |
| BspACI | CCGC | 1 cut(s) | 1145 |
| BspANI | GGCC | 4 cut(s) | 860, 1200, 1563, 1591 |
| BspCNI | CTCAG | 4 cut(s) | 616, 655, 1243, 1243 |
| BspDI | ATCGAT | 1 cut(s) | 795 |
| BspFNI | CGCG | 2 cut(s) | 823, 1411 |
| BspHI | TCATGA | 1 cut(s) | 1321 |
| BspLI | GGNNCC | 4 cut(s) | 147, 783, 1201, 1417 |
| BspMAI | CTGCAG | 1 cut(s) | 514 |
| BspPI | GGATC | 5 cut(s) | 385, 776, 789, 1931, 1977 |
| BspT107I | GGYRCC | 2 cut(s) | 145, 1415 |
| BspTI | CTTAAG | 1 cut(s) | 62 |
| BsrDI | GCAATG | 1 cut(s) | 1483 |
| BsrFI | RCCGGY | 1 cut(s) | 148 |
| BsrI | ACTGG | 2 cut(s) | 638, 2084 |
| BssAI | RCCGGY | 1 cut(s) | 148 |
| BssECI | CCNNGG | 3 cut(s) | 948, 1275, 1958 |
| BssNI | GRCGYC | 1 cut(s) | 1036 |
| BssT1I | CCWWGG | 3 cut(s) | 948, 1275, 1958 |
| Bst4CI | ACNGT | 5 cut(s) | 738, 1015, 1420, 1558, 2138 |
| Bst6I | CTCTTC | 5 cut(s) | 973, 1036, 1050, 1767, 1896 |
| BstACI | GRCGYC | 1 cut(s) | 1036 |
| BstAFI | CTTAAG | 1 cut(s) | 62 |
| BstC8I | GCNNGC | 3 cut(s) | 382, 465, 627 |
| BstDEI | CTNAG | 6 cut(s) | 141, 603, 663, 786, 1230, 1251 |
| BstDSI | CCRYGG | 2 cut(s) | 948, 1958 |
| BstEII | GGTNACC | 1 cut(s) | 1788 |
| BstF5I | GGATG | 6 cut(s) | 430, 918, 962, 1203, 2070, 2080 |
| BstFNI | CGCG | 2 cut(s) | 823, 1411 |
| BstHHI | GCGC | 3 cut(s) | 160, 823, 1411 |
| BstMAI | GTCTC | 3 cut(s) | 191, 694, 2087 |
| BstMCI | CGRYCG | 1 cut(s) | 799 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 1166, 1244 |
| BstNSI | RCATGY | 2 cut(s) | 210, 2153 |
| BstPI | GGTNACC | 1 cut(s) | 1788 |
| BstSFI | CTRYAG | 3 cut(s) | 510, 1557, 1569 |
| BstUI | CGCG | 2 cut(s) | 823, 1411 |
| BstV1I | GCAGC | 5 cut(s) | 3, 356, 524, 1169, 1181 |
| BstV2I | GAAGAC | 3 cut(s) | 18, 582, 642 |
| BstX2I | RGATCY | 4 cut(s) | 390, 781, 1126, 1982 |
| BstXI | CCANNNNNNTGG | 2 cut(s) | 868, 955 |
| BstYI | RGATCY | 4 cut(s) | 390, 781, 1126, 1982 |
| Bsu15I | ATCGAT | 1 cut(s) | 795 |
| Bsu36I | CCTNAGG | 3 cut(s) | 141, 603, 786 |
| BsuI | GTATCC | 1 cut(s) | 729 |
| BsuRI | GGCC | 4 cut(s) | 860, 1200, 1563, 1591 |
| BsuTUI | ATCGAT | 1 cut(s) | 795 |
| BtgI | CCRYGG | 2 cut(s) | 948, 1958 |
| BtgZI | GCGATG | 1 cut(s) | 251 |
| BtsCI | GGATG | 6 cut(s) | 430, 918, 962, 1203, 2070, 2080 |
| BtsIMutI | CAGTG | 2 cut(s) | 1554, 2143 |
| Cac8I | GCNNGC | 3 cut(s) | 382, 465, 627 |
| CciI | TCATGA | 1 cut(s) | 1321 |
| CfoI | GCGC | 3 cut(s) | 160, 823, 1411 |
| Cfr10I | RCCGGY | 1 cut(s) | 148 |
| Cfr13I | GGNCC | 1 cut(s) | 1199 |
| ClaI | ATCGAT | 1 cut(s) | 795 |
| CseI | GACGC | 2 cut(s) | 1044, 1517 |
| Csp6I | GTAC | 6 cut(s) | 313, 610, 1224, 1416, 1421, 1882 |
| CspAI | ACCGGT | 1 cut(s) | 148 |
| CviQI | GTAC | 6 cut(s) | 313, 610, 1224, 1416, 1421, 1882 |
| DdeI | CTNAG | 6 cut(s) | 141, 603, 663, 786, 1230, 1251 |
| DraI | TTTAAA | 1 cut(s) | 571 |
| Eam1104I | CTCTTC | 5 cut(s) | 973, 1036, 1050, 1767, 1896 |
| EarI | CTCTTC | 5 cut(s) | 973, 1036, 1050, 1767, 1896 |
| EciI | GGCGGA | 1 cut(s) | 1160 |
| Eco130I | CCWWGG | 3 cut(s) | 948, 1275, 1958 |
| Eco32I | GATATC | 2 cut(s) | 793, 1822 |
| Eco57I | CTGAAG | 1 cut(s) | 1533 |
| Eco81I | CCTNAGG | 3 cut(s) | 141, 603, 786 |
| Eco91I | GGTNACC | 1 cut(s) | 1788 |
| EcoO65I | GGTNACC | 1 cut(s) | 1788 |
| EcoRI | GAATTC | 1 cut(s) | 530 |
| EcoRV | GATATC | 2 cut(s) | 793, 1822 |
| EcoT14I | CCWWGG | 3 cut(s) | 948, 1275, 1958 |
| EcoT22I | ATGCAT | 2 cut(s) | 267, 1249 |
| ErhI | CCWWGG | 3 cut(s) | 948, 1275, 1958 |
| Esp3I | CGTCTC | 2 cut(s) | 694, 2087 |
| FaqI | GGGAC | 1 cut(s) | 1981 |
| FbaI | TGATCA | 2 cut(s) | 2023, 2056 |
| FblI | GTMKAC | 1 cut(s) | 1386 |
| Fnu4HI | GCNGC | 5 cut(s) | 17, 345, 513, 1158, 1170 |
| FokI | GGATG | 6 cut(s) | 437, 925, 949, 1190, 2067, 2077 |
| Fsp4HI | GCNGC | 5 cut(s) | 17, 345, 513, 1158, 1170 |
| FspAI | RTGCGCAY | 1 cut(s) | 159 |
| FspBI | CTAG | 8 cut(s) | 84, 540, 654, 1032, 1260, 1424, 1601, 1629 |
| FspI | TGCGCA | 1 cut(s) | 159 |
| GlaI | GCGC | 3 cut(s) | 159, 822, 1410 |
| GluI | GCNGC | 5 cut(s) | 17, 345, 513, 1158, 1170 |
| GsaI | CCCAGC | 1 cut(s) | 1242 |
| HaeIII | GGCC | 4 cut(s) | 860, 1200, 1563, 1591 |
| HapII | CCGG | 2 cut(s) | 149, 2052 |
| HgaI | GACGC | 2 cut(s) | 1044, 1517 |
| HhaI | GCGC | 3 cut(s) | 160, 823, 1411 |
| Hin1I | GRCGYC | 1 cut(s) | 1036 |
| Hin6I | GCGC | 3 cut(s) | 158, 821, 1409 |
| HinP1I | GCGC | 3 cut(s) | 158, 821, 1409 |
| HincII | GTYRAC | 5 cut(s) | 102, 329, 1387, 2070, 2110 |
| HindII | GTYRAC | 5 cut(s) | 102, 329, 1387, 2070, 2110 |
| HindIII | AAGCTT | 1 cut(s) | 443 |
| HinfI | GANTC | 7 cut(s) | 431, 452, 1006, 1295, 1624, 2004, 2111 |
| HpaI | GTTAAC | 1 cut(s) | 2070 |
| HpaII | CCGG | 2 cut(s) | 149, 2052 |
| HphI | GGTGA | 3 cut(s) | 588, 1782, 2066 |
| Hpy166II | GTNNAC | 6 cut(s) | 102, 329, 1387, 1678, 2070, 2110 |
| Hpy188I | TCNGA | 9 cut(s) | 58, 484, 664, 967, 1233, 1252, 1300, 2028, 2124 |
| Hpy188III | TCNNGA | 5 cut(s) | 540, 605, 837, 1322, 1810 |
| Hpy8I | GTNNAC | 6 cut(s) | 102, 329, 1387, 1678, 2070, 2110 |
| HpyAV | CCTTC | 2 cut(s) | 232, 913 |
| HpyCH4III | ACNGT | 5 cut(s) | 738, 1015, 1420, 1558, 2138 |
| HpyCH4IV | ACGT | 1 cut(s) | 1222 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 1166, 1244 |
| HpyF3I | CTNAG | 6 cut(s) | 141, 603, 663, 786, 1230, 1251 |
| HpySE526I | ACGT | 1 cut(s) | 1222 |
| Hsp92I | GRCGYC | 1 cut(s) | 1036 |
| HspAI | GCGC | 3 cut(s) | 158, 821, 1409 |
| KpnI | GGTACC | 1 cut(s) | 1419 |
| Ksp22I | TGATCA | 2 cut(s) | 2023, 2056 |
| KspAI | GTTAAC | 1 cut(s) | 2070 |
| LmnI | GCTCC | 2 cut(s) | 1960, 2012 |
| Lsp1109I | GCAGC | 5 cut(s) | 3, 356, 524, 1169, 1181 |
| LweI | GCATC | 5 cut(s) | 274, 345, 415, 1256, 1423 |
| MaeI | CTAG | 8 cut(s) | 84, 540, 654, 1032, 1260, 1424, 1601, 1629 |
| MaeII | ACGT | 1 cut(s) | 1222 |
| MaeIII | GTNAC | 4 cut(s) | 732, 1003, 1120, 1788 |
| MfeI | CAATTG | 1 cut(s) | 866 |
| MflI | RGATCY | 4 cut(s) | 390, 781, 1126, 1982 |
| MhlI | GDGCHC | 1 cut(s) | 2017 |
| MlyI | GAGTC | 3 cut(s) | 440, 1000, 2105 |
| MmeI | TCCRAC | 2 cut(s) | 431, 1034 |
| Mph1103I | ATGCAT | 2 cut(s) | 267, 1249 |
| MseI | TTAA | 9 cut(s) | 63, 570, 890, 1023, 1077, 1536, 1610, 1737, 2069 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1320 |
| MspCI | CTTAAG | 1 cut(s) | 62 |
| MspI | CCGG | 2 cut(s) | 149, 2052 |
| MunI | CAATTG | 1 cut(s) | 866 |
| Mva1269I | GAATGC | 3 cut(s) | 491, 1249, 1695 |
| MvnI | CGCG | 2 cut(s) | 823, 1411 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 1166, 1244 |
| NcoI | CCATGG | 2 cut(s) | 948, 1958 |
| NlaIV | GGNNCC | 4 cut(s) | 147, 783, 1201, 1417 |
| NmeAIII | GCCGAG | 1 cut(s) | 454 |
| NmuCI | GTSAC | 4 cut(s) | 732, 1003, 1120, 1788 |
| NsbI | TGCGCA | 1 cut(s) | 159 |
| NsiI | ATGCAT | 2 cut(s) | 267, 1249 |
| NspI | RCATGY | 2 cut(s) | 210, 2153 |
| PagI | TCATGA | 1 cut(s) | 1321 |
| PciI | ACATGT | 2 cut(s) | 206, 2149 |
| PctI | GAATGC | 3 cut(s) | 491, 1249, 1695 |
| PfeI | GAWTC | 4 cut(s) | 452, 1295, 1624, 2004 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1964 |
| PinAI | ACCGGT | 1 cut(s) | 148 |
| PkrI | GCNGC | 5 cut(s) | 18, 346, 514, 1159, 1171 |
| Ple19I | CGATCG | 1 cut(s) | 799 |
| PleI | GAGTC | 3 cut(s) | 439, 1000, 2105 |
| PpsI | GAGTC | 3 cut(s) | 439, 1000, 2105 |
| PscI | ACATGT | 2 cut(s) | 206, 2149 |
| PspEI | GGTNACC | 1 cut(s) | 1788 |
| PspFI | CCCAGC | 1 cut(s) | 1238 |
| PspN4I | GGNNCC | 4 cut(s) | 147, 783, 1201, 1417 |
| PspPI | GGNCC | 1 cut(s) | 1199 |
| PstI | CTGCAG | 1 cut(s) | 514 |
| PsuI | RGATCY | 4 cut(s) | 390, 781, 1126, 1982 |
| PvuI | CGATCG | 1 cut(s) | 799 |
| RsaI | GTAC | 6 cut(s) | 314, 611, 1225, 1417, 1422, 1883 |
| RsaNI | GTAC | 6 cut(s) | 313, 610, 1224, 1416, 1421, 1882 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1320 |
| SalI | GTCGAC | 1 cut(s) | 1385 |
| SaqAI | TTAA | 9 cut(s) | 63, 570, 890, 1023, 1077, 1536, 1610, 1737, 2069 |
| SatI | GCNGC | 5 cut(s) | 17, 345, 513, 1158, 1170 |
| Sau96I | GGNCC | 1 cut(s) | 1199 |
| SchI | GAGTC | 3 cut(s) | 440, 1000, 2105 |
| SduI | GDGCHC | 1 cut(s) | 2017 |
| SfaNI | GCATC | 5 cut(s) | 274, 345, 415, 1256, 1423 |
| SfcI | CTRYAG | 3 cut(s) | 510, 1557, 1569 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1320 |
| SmlI | CTYRAG | 1 cut(s) | 62 |
| SmoI | CTYRAG | 1 cut(s) | 62 |
| SsiI | CCGC | 1 cut(s) | 1145 |
| SspI | AATATT | 1 cut(s) | 1710 |
| SspMI | CTAG | 8 cut(s) | 84, 540, 654, 1032, 1260, 1424, 1601, 1629 |
| StyI | CCWWGG | 3 cut(s) | 948, 1275, 1958 |
| TaaI | ACNGT | 5 cut(s) | 738, 1015, 1420, 1558, 2138 |
| TaiI | ACGT | 1 cut(s) | 1225 |
| TaqI | TCGA | 4 cut(s) | 116, 795, 1386, 1795 |
| TaqII | GACCGA | 1 cut(s) | 2016 |
| TatI | WGTACW | 2 cut(s) | 312, 609 |
| TfiI | GAWTC | 4 cut(s) | 452, 1295, 1624, 2004 |
| Tru1I | TTAA | 9 cut(s) | 63, 570, 890, 1023, 1077, 1536, 1610, 1737, 2069 |
| Tru9I | TTAA | 9 cut(s) | 63, 570, 890, 1023, 1077, 1536, 1610, 1737, 2069 |
| TscAI | CASTG | 2 cut(s) | 1561, 2143 |
| TseFI | GTSAC | 4 cut(s) | 732, 1003, 1120, 1788 |
| TseI | GCWGC | 5 cut(s) | 16, 344, 512, 1157, 1169 |
| Tsp45I | GTSAC | 4 cut(s) | 732, 1003, 1120, 1788 |
| TspGWI | ACGGA | 1 cut(s) | 351 |
| TspRI | CASTG | 2 cut(s) | 1561, 2143 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1964 |
| Vha464I | CTTAAG | 1 cut(s) | 62 |
| XapI | RAATTY | 1 cut(s) | 530 |
| XbaI | TCTAGA | 1 cut(s) | 539 |
| XceI | RCATGY | 2 cut(s) | 210, 2153 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 190 |
| XmiI | GTMKAC | 1 cut(s) | 1386 |
| XspI | CTAG | 8 cut(s) | 84, 540, 654, 1032, 1260, 1424, 1601, 1629 |
| Zsp2I | ATGCAT | 2 cut(s) | 267, 1249 |