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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AclWI | GGATC | 2 cut(s) | 28, 41 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 44 |
| AgsI | TTSAA | 2 cut(s) | 15, 29 |
| AlwI | GGATC | 2 cut(s) | 28, 41 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 163 |
| BamHI | GGATCC | 1 cut(s) | 33 |
| BccI | CCATC | 1 cut(s) | 59 |
| BfaI | CTAG | 1 cut(s) | 53 |
| BmiI | GGNNCC | 1 cut(s) | 35 |
| BsaWI | WCCGGW | 1 cut(s) | 175 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 44 |
| Bse3DI | GCAATG | 1 cut(s) | 111 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 44 |
| BseMI | GCAATG | 1 cut(s) | 111 |
| BsiSI | CCGG | 1 cut(s) | 176 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 44 |
| BsmI | GAATGC | 1 cut(s) | 48 |
| Bsp143I | GATC | 1 cut(s) | 33 |
| BspLI | GGNNCC | 1 cut(s) | 35 |
| BspPI | GGATC | 2 cut(s) | 28, 41 |
| BsrDI | GCAATG | 1 cut(s) | 111 |
| BssMI | GATC | 1 cut(s) | 33 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 163 |
| BstDEI | CTNAG | 1 cut(s) | 153 |
| BstKTI | GATC | 1 cut(s) | 36 |
| BstMBI | GATC | 1 cut(s) | 33 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 163, 206 |
| BstX2I | RGATCY | 1 cut(s) | 33 |
| BstYI | RGATCY | 1 cut(s) | 33 |
| CaiI | CAGNNNCTG | 1 cut(s) | 163 |
| CviAII | CATG | 2 cut(s) | 91, 100 |
| CviJI | RGCY | 1 cut(s) | 200 |
| CviKI_1 | RGCY | 1 cut(s) | 200 |
| DdeI | CTNAG | 1 cut(s) | 153 |
| DpnI | GATC | 1 cut(s) | 35 |
| DpnII | GATC | 1 cut(s) | 33 |
| FaeI | CATG | 2 cut(s) | 94, 103 |
| FaiI | YATR | 4 cut(s) | 82, 92, 101, 173 |
| FatI | CATG | 2 cut(s) | 90, 99 |
| FspBI | CTAG | 1 cut(s) | 53 |
| HapII | CCGG | 1 cut(s) | 176 |
| Hin1II | CATG | 2 cut(s) | 94, 103 |
| HpaII | CCGG | 1 cut(s) | 176 |
| Hpy188I | TCNGA | 1 cut(s) | 222 |
| Hpy188III | TCNNGA | 2 cut(s) | 15, 53 |
| HpyAV | CCTTC | 2 cut(s) | 50, 97 |
| HpyCH4V | TGCA | 2 cut(s) | 80, 116 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 163, 206 |
| HpyF3I | CTNAG | 1 cut(s) | 153 |
| Hsp92II | CATG | 2 cut(s) | 94, 103 |
| Kzo9I | GATC | 1 cut(s) | 33 |
| LmnI | GCTCC | 1 cut(s) | 178 |
| LpnPI | CCDG | 2 cut(s) | 143, 189 |
| MaeI | CTAG | 1 cut(s) | 53 |
| MaeIII | GTNAC | 2 cut(s) | 95, 167 |
| MalI | GATC | 1 cut(s) | 35 |
| MboI | GATC | 1 cut(s) | 33 |
| MboII | GAAGA | 2 cut(s) | 3, 16 |
| MflI | RGATCY | 1 cut(s) | 33 |
| MluCI | AATT | 1 cut(s) | 183 |
| MseI | TTAA | 1 cut(s) | 228 |
| MspI | CCGG | 1 cut(s) | 176 |
| Mva1269I | GAATGC | 1 cut(s) | 48 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 163, 206 |
| NdeII | GATC | 1 cut(s) | 33 |
| NlaIII | CATG | 2 cut(s) | 94, 103 |
| NlaIV | GGNNCC | 1 cut(s) | 35 |
| PctI | GAATGC | 1 cut(s) | 48 |
| PspN4I | GGNNCC | 1 cut(s) | 35 |
| PstNI | CAGNNNCTG | 1 cut(s) | 163 |
| PsuI | RGATCY | 1 cut(s) | 33 |
| SaqAI | TTAA | 1 cut(s) | 228 |
| Sau3AI | GATC | 1 cut(s) | 33 |
| SetI | ASST | 1 cut(s) | 234 |
| Sse9I | AATT | 1 cut(s) | 183 |
| SspMI | CTAG | 1 cut(s) | 53 |
| TasI | AATT | 1 cut(s) | 183 |
| Tru1I | TTAA | 1 cut(s) | 228 |
| Tru9I | TTAA | 1 cut(s) | 228 |
| TspDTI | ATGAA | 3 cut(s) | 17, 116, 204 |
| XbaI | TCTAGA | 1 cut(s) | 52 |
| XspI | CTAG | 1 cut(s) | 53 |