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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccIII | TCCGGA | 1 cut(s) | 19 |
| AciI | CCGC | 1 cut(s) | 80 |
| AgsI | TTSAA | 1 cut(s) | 197 |
| AluBI | AGCT | 1 cut(s) | 204 |
| AluI | AGCT | 1 cut(s) | 204 |
| Aor13HI | TCCGGA | 1 cut(s) | 19 |
| AoxI | GGCC | 1 cut(s) | 165 |
| AspS9I | GGNCC | 2 cut(s) | 160, 165 |
| AsuC2I | CCSGG | 1 cut(s) | 164 |
| AvaII | GGWCC | 1 cut(s) | 160 |
| BclI | TGATCA | 1 cut(s) | 154 |
| BcnI | CCSGG | 1 cut(s) | 164 |
| BfaI | CTAG | 1 cut(s) | 205 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 173 |
| Bme1390I | CCNGG | 1 cut(s) | 164 |
| Bme18I | GGWCC | 1 cut(s) | 160 |
| BmgT120I | GGNCC | 2 cut(s) | 160, 165 |
| BmrFI | CCNGG | 1 cut(s) | 164 |
| BpuMI | CCSGG | 1 cut(s) | 164 |
| BsaWI | WCCGGW | 1 cut(s) | 19 |
| BseAI | TCCGGA | 1 cut(s) | 19 |
| BshFI | GGCC | 1 cut(s) | 167 |
| BsiSI | CCGG | 2 cut(s) | 20, 163 |
| BsmI | GAATGC | 1 cut(s) | 15 |
| BsnI | GGCC | 1 cut(s) | 167 |
| Bsp13I | TCCGGA | 1 cut(s) | 19 |
| Bsp143I | GATC | 1 cut(s) | 154 |
| BspACI | CCGC | 1 cut(s) | 80 |
| BspANI | GGCC | 1 cut(s) | 167 |
| BspEI | TCCGGA | 1 cut(s) | 19 |
| BssMI | GATC | 1 cut(s) | 154 |
| Bst4CI | ACNGT | 1 cut(s) | 51 |
| BstDEI | CTNAG | 1 cut(s) | 108 |
| BstKTI | GATC | 1 cut(s) | 157 |
| BstMBI | GATC | 1 cut(s) | 154 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 173 |
| BstSCI | CCNGG | 1 cut(s) | 162 |
| BsuRI | GGCC | 1 cut(s) | 167 |
| Cfr13I | GGNCC | 2 cut(s) | 160, 165 |
| CviAII | CATG | 1 cut(s) | 67 |
| CviJI | RGCY | 3 cut(s) | 167, 189, 204 |
| CviKI_1 | RGCY | 3 cut(s) | 167, 189, 204 |
| DdeI | CTNAG | 1 cut(s) | 108 |
| DpnI | GATC | 1 cut(s) | 156 |
| DpnII | GATC | 1 cut(s) | 154 |
| Eco47I | GGWCC | 1 cut(s) | 160 |
| EcoT22I | ATGCAT | 1 cut(s) | 63 |
| FaeI | CATG | 1 cut(s) | 70 |
| FaiI | YATR | 4 cut(s) | 59, 68, 77, 183 |
| FatI | CATG | 1 cut(s) | 66 |
| FbaI | TGATCA | 1 cut(s) | 154 |
| FspBI | CTAG | 1 cut(s) | 205 |
| HaeIII | GGCC | 1 cut(s) | 167 |
| HapII | CCGG | 2 cut(s) | 20, 163 |
| Hin1II | CATG | 1 cut(s) | 70 |
| HpaII | CCGG | 2 cut(s) | 20, 163 |
| Hpy166II | GTNNAC | 1 cut(s) | 47 |
| Hpy188III | TCNNGA | 1 cut(s) | 20 |
| Hpy8I | GTNNAC | 1 cut(s) | 47 |
| HpyCH4III | ACNGT | 1 cut(s) | 51 |
| HpyCH4V | TGCA | 1 cut(s) | 61 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 173 |
| HpyF3I | CTNAG | 1 cut(s) | 108 |
| Hsp92II | CATG | 1 cut(s) | 70 |
| Kpn2I | TCCGGA | 1 cut(s) | 19 |
| Ksp22I | TGATCA | 1 cut(s) | 154 |
| Kzo9I | GATC | 1 cut(s) | 154 |
| LmnI | GCTCC | 1 cut(s) | 22 |
| LpnPI | CCDG | 3 cut(s) | 33, 143, 176 |
| MaeI | CTAG | 1 cut(s) | 205 |
| MalI | GATC | 1 cut(s) | 156 |
| MboI | GATC | 1 cut(s) | 154 |
| MfeI | CAATTG | 1 cut(s) | 26 |
| MluCI | AATT | 2 cut(s) | 26, 191 |
| MnlI | CCTC | 1 cut(s) | 76 |
| Mph1103I | ATGCAT | 1 cut(s) | 63 |
| MroI | TCCGGA | 1 cut(s) | 19 |
| MslI | CAYNNNNRTG | 1 cut(s) | 102 |
| MspI | CCGG | 2 cut(s) | 20, 163 |
| MspR9I | CCNGG | 1 cut(s) | 164 |
| MunI | CAATTG | 1 cut(s) | 26 |
| Mva1269I | GAATGC | 1 cut(s) | 15 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 173 |
| NciI | CCSGG | 1 cut(s) | 164 |
| NdeII | GATC | 1 cut(s) | 154 |
| NlaIII | CATG | 1 cut(s) | 70 |
| NmeAIII | GCCGAG | 1 cut(s) | 193 |
| NsiI | ATGCAT | 1 cut(s) | 63 |
| PctI | GAATGC | 1 cut(s) | 15 |
| PspPI | GGNCC | 2 cut(s) | 160, 165 |
| RseI | CAYNNNNRTG | 1 cut(s) | 102 |
| Sau3AI | GATC | 1 cut(s) | 154 |
| Sau96I | GGNCC | 2 cut(s) | 160, 165 |
| ScrFI | CCNGG | 1 cut(s) | 164 |
| SetI | ASST | 2 cut(s) | 162, 206 |
| SgeI | CNNG | 8 cut(s) | 23, 32, 54, 79, 170, 175, 176, 181 |
| SinI | GGWCC | 1 cut(s) | 160 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 102 |
| Sse9I | AATT | 2 cut(s) | 26, 191 |
| SsiI | CCGC | 1 cut(s) | 80 |
| SspMI | CTAG | 1 cut(s) | 205 |
| StyD4I | CCNGG | 1 cut(s) | 162 |
| TaaI | ACNGT | 1 cut(s) | 51 |
| TasI | AATT | 2 cut(s) | 26, 191 |
| TspDTI | ATGAA | 1 cut(s) | 83 |
| VpaK11BI | GGWCC | 1 cut(s) | 160 |
| XspI | CTAG | 1 cut(s) | 205 |
| Zsp2I | ATGCAT | 1 cut(s) | 63 |