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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 541 |
| Acc36I | ACCTGC | 1 cut(s) | 541 |
| AccI | GTMKAC | 1 cut(s) | 204 |
| AciI | CCGC | 3 cut(s) | 805, 862, 887 |
| AclWI | GGATC | 1 cut(s) | 550 |
| AcoI | YGGCCR | 3 cut(s) | 192, 529, 732 |
| AcuI | CTGAAG | 1 cut(s) | 561 |
| AfaI | GTAC | 3 cut(s) | 293, 300, 833 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 445 |
| AflIII | ACRYGT | 2 cut(s) | 288, 561 |
| AgsI | TTSAA | 7 cut(s) | 94, 142, 371, 473, 635, 683, 1066 |
| AjnI | CCWGG | 1 cut(s) | 729 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 323, 355 |
| AleI | CACNNNNGTG | 1 cut(s) | 887 |
| AluBI | AGCT | 4 cut(s) | 136, 743, 875, 926 |
| AluI | AGCT | 4 cut(s) | 136, 743, 875, 926 |
| Alw21I | GWGCWC | 2 cut(s) | 138, 718 |
| AlwI | GGATC | 1 cut(s) | 550 |
| AoxI | GGCC | 7 cut(s) | 192, 456, 483, 529, 609, 732, 863 |
| ApeKI | GCWGC | 4 cut(s) | 743, 797, 872, 923 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 362 |
| AspS9I | GGNCC | 2 cut(s) | 241, 864 |
| AsuHPI | GGTGA | 5 cut(s) | 178, 265, 565, 901, 967 |
| AsuII | TTCGAA | 1 cut(s) | 1033 |
| AvaII | GGWCC | 1 cut(s) | 241 |
| BalI | TGGCCA | 3 cut(s) | 194, 531, 734 |
| BanII | GRGCYC | 2 cut(s) | 138, 654 |
| BbsI | GAAGAC | 1 cut(s) | 175 |
| Bbv12I | GWGCWC | 2 cut(s) | 138, 718 |
| BbvCI | CCTCAGC | 1 cut(s) | 927 |
| BbvI | GCAGC | 4 cut(s) | 730, 784, 884, 935 |
| BccI | CCATC | 4 cut(s) | 521, 587, 841, 955 |
| BciT130I | CCWGG | 1 cut(s) | 731 |
| BclI | TGATCA | 1 cut(s) | 154 |
| BfuAI | ACCTGC | 1 cut(s) | 541 |
| BisI | GCNGC | 5 cut(s) | 744, 798, 863, 873, 924 |
| BlsI | GCNGC | 5 cut(s) | 745, 799, 864, 874, 925 |
| BmcAI | AGTACT | 1 cut(s) | 833 |
| Bme1390I | CCNGG | 1 cut(s) | 731 |
| Bme18I | GGWCC | 1 cut(s) | 241 |
| BmgT120I | GGNCC | 2 cut(s) | 241, 864 |
| BmiI | GGNNCC | 1 cut(s) | 467 |
| BmrFI | CCNGG | 1 cut(s) | 731 |
| BmsI | GCATC | 4 cut(s) | 52, 156, 220, 811 |
| BpiI | GAAGAC | 1 cut(s) | 175 |
| BpmI | CTGGAG | 2 cut(s) | 292, 740 |
| Bpu10I | CCTNAGC | 1 cut(s) | 927 |
| Bpu14I | TTCGAA | 1 cut(s) | 1033 |
| BpuEI | CTTGAG | 3 cut(s) | 221, 677, 860 |
| BsaAI | YACGTR | 1 cut(s) | 291 |
| BsaJI | CCNNGG | 6 cut(s) | 189, 225, 459, 486, 612, 708 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 445 |
| Bse118I | RCCGGY | 1 cut(s) | 445 |
| Bse1I | ACTGG | 1 cut(s) | 271 |
| Bse3DI | GCAATG | 4 cut(s) | 429, 552, 592, 999 |
| BseBI | CCWGG | 1 cut(s) | 731 |
| BseDI | CCNNGG | 6 cut(s) | 189, 225, 459, 486, 612, 708 |
| BseGI | GGATG | 5 cut(s) | 235, 393, 598, 610, 937 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 445 |
| BseMI | GCAATG | 4 cut(s) | 429, 552, 592, 999 |
| BseMII | CTCAG | 1 cut(s) | 918 |
| BseNI | ACTGG | 1 cut(s) | 271 |
| BseRI | GAGGAG | 1 cut(s) | 467 |
| BseXI | GCAGC | 4 cut(s) | 730, 784, 884, 935 |
| BseYI | CCCAGC | 1 cut(s) | 746 |
| BshFI | GGCC | 7 cut(s) | 194, 458, 485, 531, 611, 734, 865 |
| BsiHKAI | GWGCWC | 2 cut(s) | 138, 718 |
| BsiSI | CCGG | 3 cut(s) | 111, 170, 446 |
| BslFI | GGGAC | 2 cut(s) | 68, 804 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 445 |
| BsmFI | GGGAC | 2 cut(s) | 68, 804 |
| BsmI | GAATGC | 2 cut(s) | 430, 725 |
| BsnI | GGCC | 7 cut(s) | 194, 458, 485, 531, 611, 734, 865 |
| Bsp119I | TTCGAA | 1 cut(s) | 1033 |
| Bsp1286I | GDGCHC | 3 cut(s) | 138, 654, 718 |
| Bsp143I | GATC | 3 cut(s) | 154, 555, 571 |
| Bsp19I | CCATGG | 2 cut(s) | 189, 225 |
| BspACI | CCGC | 3 cut(s) | 805, 862, 887 |
| BspANI | GGCC | 7 cut(s) | 194, 458, 485, 531, 611, 734, 865 |
| BspCNI | CTCAG | 1 cut(s) | 919 |
| BspHI | TCATGA | 1 cut(s) | 1041 |
| BspLI | GGNNCC | 1 cut(s) | 467 |
| BspMI | ACCTGC | 1 cut(s) | 541 |
| BspPI | GGATC | 1 cut(s) | 550 |
| BspQI | GCTCTTC | 1 cut(s) | 143 |
| BspT104I | TTCGAA | 1 cut(s) | 1033 |
| BsrDI | GCAATG | 4 cut(s) | 429, 552, 592, 999 |
| BsrFI | RCCGGY | 1 cut(s) | 445 |
| BsrI | ACTGG | 1 cut(s) | 271 |
| BssAI | RCCGGY | 1 cut(s) | 445 |
| BssECI | CCNNGG | 6 cut(s) | 189, 225, 459, 486, 612, 708 |
| BssMI | GATC | 3 cut(s) | 154, 555, 571 |
| BssT1I | CCWWGG | 5 cut(s) | 189, 225, 459, 486, 708 |
| Bst2UI | CCWGG | 1 cut(s) | 731 |
| Bst4CI | ACNGT | 3 cut(s) | 14, 298, 778 |
| Bst6I | CTCTTC | 1 cut(s) | 143 |
| BstBAI | YACGTR | 1 cut(s) | 291 |
| BstBI | TTCGAA | 1 cut(s) | 1033 |
| BstC8I | GCNNGC | 1 cut(s) | 217 |
| BstDEI | CTNAG | 2 cut(s) | 825, 927 |
| BstDSI | CCRYGG | 3 cut(s) | 189, 225, 612 |
| BstF5I | GGATG | 5 cut(s) | 235, 393, 598, 610, 937 |
| BstKTI | GATC | 3 cut(s) | 157, 558, 574 |
| BstMBI | GATC | 3 cut(s) | 154, 555, 571 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 71, 142, 158, 722, 731, 740, 752 |
| BstNI | CCWGG | 1 cut(s) | 731 |
| BstNSI | RCATGY | 1 cut(s) | 565 |
| BstSCI | CCNGG | 1 cut(s) | 729 |
| BstV1I | GCAGC | 4 cut(s) | 730, 784, 884, 935 |
| BstV2I | GAAGAC | 1 cut(s) | 175 |
| BsuRI | GGCC | 7 cut(s) | 194, 458, 485, 531, 611, 734, 865 |
| BtgI | CCRYGG | 3 cut(s) | 189, 225, 612 |
| BtsCI | GGATG | 5 cut(s) | 235, 393, 598, 610, 937 |
| BtsI | GCAGTG | 1 cut(s) | 882 |
| BtsIMutI | CAGTG | 3 cut(s) | 264, 537, 882 |
| BveI | ACCTGC | 1 cut(s) | 541 |
| Cac8I | GCNNGC | 1 cut(s) | 217 |
| CciI | TCATGA | 1 cut(s) | 1041 |
| Cfr10I | RCCGGY | 1 cut(s) | 445 |
| Cfr13I | GGNCC | 2 cut(s) | 241, 864 |
| Csp6I | GTAC | 3 cut(s) | 292, 299, 832 |
| CviAII | CATG | 8 cut(s) | 46, 190, 226, 251, 256, 562, 736, 1042 |
| CviQI | GTAC | 3 cut(s) | 292, 299, 832 |
| DdeI | CTNAG | 2 cut(s) | 825, 927 |
| DpnI | GATC | 3 cut(s) | 156, 557, 573 |
| DpnII | GATC | 3 cut(s) | 154, 555, 571 |
| DraI | TTTAAA | 1 cut(s) | 1008 |
| EaeI | YGGCCR | 3 cut(s) | 192, 529, 732 |
| Eam1104I | CTCTTC | 1 cut(s) | 143 |
| EarI | CTCTTC | 1 cut(s) | 143 |
| Ecl136II | GAGCTC | 1 cut(s) | 136 |
| Eco130I | CCWWGG | 5 cut(s) | 189, 225, 459, 486, 708 |
| Eco24I | GRGCYC | 2 cut(s) | 138, 654 |
| Eco47I | GGWCC | 1 cut(s) | 241 |
| Eco53kI | GAGCTC | 1 cut(s) | 136 |
| Eco57I | CTGAAG | 1 cut(s) | 561 |
| EcoICRI | GAGCTC | 1 cut(s) | 136 |
| EcoRII | CCWGG | 1 cut(s) | 729 |
| EcoT14I | CCWWGG | 5 cut(s) | 189, 225, 459, 486, 708 |
| EcoT38I | GRGCYC | 2 cut(s) | 138, 654 |
| ErhI | CCWWGG | 5 cut(s) | 189, 225, 459, 486, 708 |
| FaeI | CATG | 8 cut(s) | 49, 193, 229, 254, 259, 565, 739, 1045 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 818, 850 |
| FaqI | GGGAC | 2 cut(s) | 68, 804 |
| FatI | CATG | 8 cut(s) | 45, 189, 225, 250, 255, 561, 735, 1041 |
| FbaI | TGATCA | 1 cut(s) | 154 |
| FblI | GTMKAC | 1 cut(s) | 204 |
| Fnu4HI | GCNGC | 5 cut(s) | 744, 798, 863, 873, 924 |
| FokI | GGATG | 5 cut(s) | 242, 400, 605, 617, 944 |
| FriOI | GRGCYC | 2 cut(s) | 138, 654 |
| Fsp4HI | GCNGC | 5 cut(s) | 744, 798, 863, 873, 924 |
| GluI | GCNGC | 5 cut(s) | 744, 798, 863, 873, 924 |
| GsaI | CCCAGC | 1 cut(s) | 750 |
| GsuI | CTGGAG | 2 cut(s) | 292, 740 |
| HaeIII | GGCC | 7 cut(s) | 194, 458, 485, 531, 611, 734, 865 |
| HapII | CCGG | 3 cut(s) | 111, 170, 446 |
| Hin1II | CATG | 8 cut(s) | 49, 193, 229, 254, 259, 565, 739, 1045 |
| HinfI | GANTC | 2 cut(s) | 107, 792 |
| HpaII | CCGG | 3 cut(s) | 111, 170, 446 |
| HphI | GGTGA | 5 cut(s) | 178, 265, 565, 901, 967 |
| Hpy166II | GTNNAC | 4 cut(s) | 205, 244, 294, 580 |
| Hpy188III | TCNNGA | 3 cut(s) | 309, 719, 1042 |
| Hpy8I | GTNNAC | 4 cut(s) | 205, 244, 294, 580 |
| Hpy99I | CGWCG | 1 cut(s) | 347 |
| HpyAV | CCTTC | 4 cut(s) | 634, 908, 929, 1029 |
| HpyCH4III | ACNGT | 3 cut(s) | 14, 298, 778 |
| HpyCH4IV | ACGT | 1 cut(s) | 290 |
| HpyCH4V | TGCA | 7 cut(s) | 74, 317, 550, 702, 872, 905, 986 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 71, 142, 158, 722, 731, 740, 752 |
| HpyF3I | CTNAG | 2 cut(s) | 825, 927 |
| HpySE526I | ACGT | 1 cut(s) | 290 |
| Hsp92II | CATG | 8 cut(s) | 49, 193, 229, 254, 259, 565, 739, 1045 |
| Ksp22I | TGATCA | 1 cut(s) | 154 |
| Kzo9I | GATC | 3 cut(s) | 154, 555, 571 |
| LguI | GCTCTTC | 1 cut(s) | 143 |
| LmnI | GCTCC | 1 cut(s) | 760 |
| Lsp1109I | GCAGC | 4 cut(s) | 730, 784, 884, 935 |
| LweI | GCATC | 4 cut(s) | 52, 156, 220, 811 |
| MaeII | ACGT | 1 cut(s) | 290 |
| MaeIII | GTNAC | 1 cut(s) | 271 |
| MalI | GATC | 3 cut(s) | 156, 557, 573 |
| MboI | GATC | 3 cut(s) | 154, 555, 571 |
| MboII | GAAGA | 3 cut(s) | 130, 175, 367 |
| MhlI | GDGCHC | 3 cut(s) | 138, 654, 718 |
| MlsI | TGGCCA | 3 cut(s) | 194, 531, 734 |
| MluCI | AATT | 2 cut(s) | 94, 331 |
| MluNI | TGGCCA | 3 cut(s) | 194, 531, 734 |
| MlyI | GAGTC | 1 cut(s) | 786 |
| MmeI | TCCRAC | 1 cut(s) | 357 |
| Mox20I | TGGCCA | 3 cut(s) | 194, 531, 734 |
| MroXI | GAANNNNTTC | 1 cut(s) | 362 |
| MscI | TGGCCA | 3 cut(s) | 194, 531, 734 |
| MseI | TTAA | 3 cut(s) | 69, 782, 1007 |
| MslI | CAYNNNNRTG | 3 cut(s) | 249, 585, 887 |
| Msp20I | TGGCCA | 3 cut(s) | 194, 531, 734 |
| MspA1I | CMGCKG | 1 cut(s) | 926 |
| MspI | CCGG | 3 cut(s) | 111, 170, 446 |
| MspR9I | CCNGG | 1 cut(s) | 731 |
| Mva1269I | GAATGC | 2 cut(s) | 430, 725 |
| MvaI | CCWGG | 1 cut(s) | 731 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 71, 142, 158, 722, 731, 740, 752 |
| NcoI | CCATGG | 2 cut(s) | 189, 225 |
| NdeII | GATC | 3 cut(s) | 154, 555, 571 |
| NlaIII | CATG | 8 cut(s) | 49, 193, 229, 254, 259, 565, 739, 1045 |
| NlaIV | GGNNCC | 1 cut(s) | 467 |
| NmuCI | GTSAC | 1 cut(s) | 271 |
| NspI | RCATGY | 1 cut(s) | 565 |
| NspV | TTCGAA | 1 cut(s) | 1033 |
| OliI | CACNNNNGTG | 1 cut(s) | 887 |
| PagI | TCATGA | 1 cut(s) | 1041 |
| PaqCI | CACCTGC | 1 cut(s) | 541 |
| PciI | ACATGT | 1 cut(s) | 561 |
| PciSI | GCTCTTC | 1 cut(s) | 143 |
| PctI | GAATGC | 2 cut(s) | 430, 725 |
| PdmI | GAANNNNTTC | 1 cut(s) | 362 |
| PfeI | GAWTC | 1 cut(s) | 107 |
| PkrI | GCNGC | 5 cut(s) | 745, 799, 864, 874, 925 |
| PleI | GAGTC | 1 cut(s) | 786 |
| PpsI | GAGTC | 1 cut(s) | 786 |
| Ppu21I | YACGTR | 1 cut(s) | 291 |
| PscI | ACATGT | 1 cut(s) | 561 |
| Psp124BI | GAGCTC | 1 cut(s) | 138 |
| Psp6I | CCWGG | 1 cut(s) | 729 |
| PspFI | CCCAGC | 1 cut(s) | 746 |
| PspGI | CCWGG | 1 cut(s) | 729 |
| PspN4I | GGNNCC | 1 cut(s) | 467 |
| PspPI | GGNCC | 2 cut(s) | 241, 864 |
| PvuII | CAGCTG | 1 cut(s) | 926 |
| RsaI | GTAC | 3 cut(s) | 293, 300, 833 |
| RsaNI | GTAC | 3 cut(s) | 292, 299, 832 |
| RseI | CAYNNNNRTG | 3 cut(s) | 249, 585, 887 |
| SacI | GAGCTC | 1 cut(s) | 138 |
| SapI | GCTCTTC | 1 cut(s) | 143 |
| SaqAI | TTAA | 3 cut(s) | 69, 782, 1007 |
| SatI | GCNGC | 5 cut(s) | 744, 798, 863, 873, 924 |
| Sau3AI | GATC | 3 cut(s) | 154, 555, 571 |
| Sau96I | GGNCC | 2 cut(s) | 241, 864 |
| ScaI | AGTACT | 1 cut(s) | 833 |
| SchI | GAGTC | 1 cut(s) | 786 |
| ScrFI | CCNGG | 1 cut(s) | 731 |
| SduI | GDGCHC | 3 cut(s) | 138, 654, 718 |
| SfaNI | GCATC | 4 cut(s) | 52, 156, 220, 811 |
| SfuI | TTCGAA | 1 cut(s) | 1033 |
| SinI | GGWCC | 1 cut(s) | 241 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 249, 585, 887 |
| SmlI | CTYRAG | 3 cut(s) | 236, 692, 839 |
| SmoI | CTYRAG | 3 cut(s) | 236, 692, 839 |
| Sse9I | AATT | 2 cut(s) | 94, 331 |
| SsiI | CCGC | 3 cut(s) | 805, 862, 887 |
| SspI | AATATT | 1 cut(s) | 1048 |
| SstI | GAGCTC | 1 cut(s) | 138 |
| StyD4I | CCNGG | 1 cut(s) | 729 |
| StyI | CCWWGG | 5 cut(s) | 189, 225, 459, 486, 708 |
| TaaI | ACNGT | 3 cut(s) | 14, 298, 778 |
| TaiI | ACGT | 1 cut(s) | 293 |
| TaqI | TCGA | 3 cut(s) | 517, 786, 1033 |
| TasI | AATT | 2 cut(s) | 94, 331 |
| TatI | WGTACW | 1 cut(s) | 831 |
| TauI | GCSGC | 1 cut(s) | 865 |
| TfiI | GAWTC | 1 cut(s) | 107 |
| Tru1I | TTAA | 3 cut(s) | 69, 782, 1007 |
| Tru9I | TTAA | 3 cut(s) | 69, 782, 1007 |
| TscAI | CASTG | 3 cut(s) | 271, 544, 889 |
| TseFI | GTSAC | 1 cut(s) | 271 |
| TseI | GCWGC | 4 cut(s) | 743, 797, 872, 923 |
| Tsp45I | GTSAC | 1 cut(s) | 271 |
| TspDTI | ATGAA | 6 cut(s) | 74, 138, 404, 437, 948, 1058 |
| TspGWI | ACGGA | 1 cut(s) | 629 |
| TspRI | CASTG | 3 cut(s) | 271, 544, 889 |
| VpaK11BI | GGWCC | 1 cut(s) | 241 |
| XceI | RCATGY | 1 cut(s) | 565 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 196, 715 |
| XmiI | GTMKAC | 1 cut(s) | 204 |
| XmnI | GAANNNNTTC | 1 cut(s) | 362 |
| ZrmI | AGTACT | 1 cut(s) | 833 |