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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 1786 |
| Acc16I | TGCGCA | 1 cut(s) | 384 |
| Acc36I | ACCTGC | 2 cut(s) | 589, 622 |
| AccB1I | GGYRCC | 1 cut(s) | 28 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 787 |
| AccII | CGCG | 2 cut(s) | 736, 1787 |
| AccIII | TCCGGA | 1 cut(s) | 1057 |
| AciI | CCGC | 6 cut(s) | 114, 324, 394, 869, 1244, 1549 |
| AclWI | GGATC | 3 cut(s) | 128, 1494, 1556 |
| AcoI | YGGCCR | 3 cut(s) | 325, 778, 982 |
| AcsI | RAATTY | 4 cut(s) | 965, 1349, 1622, 1855 |
| AcuI | CTGAAG | 1 cut(s) | 785 |
| AcvI | CACGTG | 1 cut(s) | 980 |
| AfaI | GTAC | 5 cut(s) | 582, 591, 751, 911, 1908 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 221, 787, 833, 1313 |
| AgsI | TTSAA | 8 cut(s) | 409, 433, 452, 970, 996, 1255, 1354, 1449 |
| AjiI | CACGTC | 2 cut(s) | 1402, 1889 |
| AjnI | CCWGG | 3 cut(s) | 202, 721, 1294 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1341, 1373 |
| AleI | CACNNNNGTG | 2 cut(s) | 34, 711 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 1708, 1740 |
| AluBI | AGCT | 8 cut(s) | 74, 501, 529, 547, 747, 866, 1042, 1166 |
| AluI | AGCT | 8 cut(s) | 74, 501, 529, 547, 747, 866, 1042, 1166 |
| Alw21I | GWGCWC | 3 cut(s) | 76, 1035, 1168 |
| Alw26I | GTCTC | 4 cut(s) | 335, 404, 1686, 1700 |
| AlwI | GGATC | 3 cut(s) | 128, 1494, 1556 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 1737 |
| Ama87I | CYCGRG | 1 cut(s) | 1683 |
| Aor13HI | TCCGGA | 1 cut(s) | 1057 |
| AoxI | GGCC | 5 cut(s) | 325, 778, 789, 946, 982 |
| ApeKI | GCWGC | 8 cut(s) | 152, 458, 554, 595, 866, 974, 1534, 1595 |
| ApoI | RAATTY | 4 cut(s) | 965, 1349, 1622, 1855 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1855 |
| AspLEI | GCGC | 1 cut(s) | 385 |
| AspS9I | GGNCC | 4 cut(s) | 276, 790, 871, 947 |
| AsuC2I | CCSGG | 2 cut(s) | 1154, 1314 |
| AsuHPI | GGTGA | 4 cut(s) | 110, 287, 698, 1302 |
| AsuII | TTCGAA | 1 cut(s) | 44 |
| AvaI | CYCGRG | 1 cut(s) | 1683 |
| AvaII | GGWCC | 2 cut(s) | 276, 871 |
| BalI | TGGCCA | 2 cut(s) | 780, 984 |
| BanI | GGYRCC | 1 cut(s) | 28 |
| BanII | GRGCYC | 3 cut(s) | 76, 1168, 1423 |
| BbrPI | CACGTG | 1 cut(s) | 980 |
| BbsI | GAAGAC | 3 cut(s) | 158, 765, 1662 |
| Bbv12I | GWGCWC | 3 cut(s) | 76, 1035, 1168 |
| BbvI | GCAGC | 8 cut(s) | 164, 470, 541, 607, 853, 986, 1521, 1607 |
| BccI | CCATC | 5 cut(s) | 111, 299, 364, 1062, 1706 |
| BceAI | ACGGC | 2 cut(s) | 312, 1547 |
| BciT130I | CCWGG | 3 cut(s) | 204, 723, 1296 |
| BciVI | GTATCC | 2 cut(s) | 131, 855 |
| BcnI | CCSGG | 2 cut(s) | 1154, 1314 |
| BcoDI | GTCTC | 4 cut(s) | 335, 404, 1686, 1700 |
| BfaI | CTAG | 2 cut(s) | 413, 1652 |
| BfmI | CTRYAG | 1 cut(s) | 1478 |
| BfuAI | ACCTGC | 2 cut(s) | 589, 622 |
| BfuI | GTATCC | 2 cut(s) | 131, 855 |
| BlpI | GCTNAGC | 1 cut(s) | 525 |
| Bme1390I | CCNGG | 5 cut(s) | 204, 723, 1154, 1296, 1314 |
| Bme18I | GGWCC | 2 cut(s) | 276, 871 |
| BmeT110I | CYCGRG | 1 cut(s) | 1683 |
| BmgBI | CACGTC | 2 cut(s) | 1402, 1889 |
| BmgT120I | GGNCC | 4 cut(s) | 276, 790, 871, 947 |
| BmiI | GGNNCC | 3 cut(s) | 30, 375, 792 |
| BmrFI | CCNGG | 5 cut(s) | 204, 723, 1154, 1296, 1314 |
| BmrI | ACTGGG | 1 cut(s) | 88 |
| BmsI | GCATC | 6 cut(s) | 370, 916, 1273, 1515, 1546, 1582 |
| BmuI | ACTGGG | 1 cut(s) | 88 |
| BpiI | GAAGAC | 3 cut(s) | 158, 765, 1662 |
| BpmI | CTGGAG | 2 cut(s) | 1209, 1387 |
| Bpu10I | CCTNAGC | 1 cut(s) | 474 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 525 |
| Bpu14I | TTCGAA | 1 cut(s) | 44 |
| BpuMI | CCSGG | 2 cut(s) | 1154, 1314 |
| BsaAI | YACGTR | 1 cut(s) | 980 |
| BsaBI | GATNNNNATC | 1 cut(s) | 843 |
| BsaI | GGTCTC | 1 cut(s) | 404 |
| BsaJI | CCNNGG | 3 cut(s) | 959, 1143, 1294 |
| BsaWI | WCCGGW | 1 cut(s) | 1057 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 221, 787, 833, 1313 |
| Bse1I | ACTGG | 6 cut(s) | 83, 100, 781, 989, 1192, 1370 |
| Bse8I | GATNNNNATC | 1 cut(s) | 843 |
| BseAI | TCCGGA | 1 cut(s) | 1057 |
| BseBI | CCWGG | 3 cut(s) | 204, 723, 1296 |
| BseDI | CCNNGG | 3 cut(s) | 959, 1143, 1294 |
| BseGI | GGATG | 5 cut(s) | 122, 823, 1054, 1722, 1882 |
| BseJI | GATNNNNATC | 1 cut(s) | 843 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 221, 787, 833, 1313 |
| BseMII | CTCAG | 5 cut(s) | 516, 724, 757, 1436, 1602 |
| BseNI | ACTGG | 6 cut(s) | 83, 100, 781, 989, 1192, 1370 |
| BseRI | GAGGAG | 1 cut(s) | 587 |
| BseX3I | CGGCCG | 1 cut(s) | 325 |
| BseXI | GCAGC | 8 cut(s) | 164, 470, 541, 607, 853, 986, 1521, 1607 |
| BseYI | CCCAGC | 1 cut(s) | 1416 |
| BsgI | GTGCAG | 1 cut(s) | 171 |
| Bsh1236I | CGCG | 2 cut(s) | 736, 1787 |
| Bsh1285I | CGRYCG | 1 cut(s) | 328 |
| BshFI | GGCC | 5 cut(s) | 327, 780, 791, 948, 984 |
| BshNI | GGYRCC | 1 cut(s) | 28 |
| BsiEI | CGRYCG | 1 cut(s) | 328 |
| BsiHKAI | GWGCWC | 3 cut(s) | 76, 1035, 1168 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1683 |
| BsiSI | CCGG | 4 cut(s) | 848, 1058, 1153, 1314 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 221, 787, 833, 1313 |
| BsmAI | GTCTC | 4 cut(s) | 335, 404, 1686, 1700 |
| BsmBI | CGTCTC | 1 cut(s) | 335 |
| BsmI | GAATGC | 3 cut(s) | 662, 1508, 1757 |
| BsnI | GGCC | 5 cut(s) | 327, 780, 791, 948, 984 |
| Bso31I | GGTCTC | 1 cut(s) | 404 |
| BsoBI | CYCGRG | 1 cut(s) | 1683 |
| Bsp119I | TTCGAA | 1 cut(s) | 44 |
| Bsp1286I | GDGCHC | 4 cut(s) | 76, 1035, 1168, 1423 |
| Bsp13I | TCCGGA | 1 cut(s) | 1057 |
| Bsp1407I | TGTACA | 1 cut(s) | 589 |
| Bsp143I | GATC | 8 cut(s) | 13, 120, 195, 292, 784, 1105, 1486, 1561 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 525 |
| Bsp19I | CCATGG | 1 cut(s) | 959 |
| BspACI | CCGC | 6 cut(s) | 114, 324, 394, 869, 1244, 1549 |
| BspANI | GGCC | 5 cut(s) | 327, 780, 791, 948, 984 |
| BspCNI | CTCAG | 5 cut(s) | 517, 723, 756, 1435, 1601 |
| BspEI | TCCGGA | 1 cut(s) | 1057 |
| BspFNI | CGCG | 2 cut(s) | 736, 1787 |
| BspLI | GGNNCC | 3 cut(s) | 30, 375, 792 |
| BspMAI | CTGCAG | 1 cut(s) | 1482 |
| BspMI | ACCTGC | 2 cut(s) | 589, 622 |
| BspPI | GGATC | 3 cut(s) | 128, 1494, 1556 |
| BspT104I | TTCGAA | 1 cut(s) | 44 |
| BspT107I | GGYRCC | 1 cut(s) | 28 |
| BspTNI | GGTCTC | 1 cut(s) | 404 |
| BsrGI | TGTACA | 1 cut(s) | 589 |
| BsrI | ACTGG | 6 cut(s) | 83, 100, 781, 989, 1192, 1370 |
| BssECI | CCNNGG | 3 cut(s) | 959, 1143, 1294 |
| BssMI | GATC | 8 cut(s) | 13, 120, 195, 292, 784, 1105, 1486, 1561 |
| BssT1I | CCWWGG | 1 cut(s) | 959 |
| Bst2UI | CCWGG | 3 cut(s) | 204, 723, 1296 |
| Bst4CI | ACNGT | 4 cut(s) | 685, 701, 1343, 1783 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1503 |
| BstAUI | TGTACA | 1 cut(s) | 589 |
| BstBAI | YACGTR | 1 cut(s) | 980 |
| BstBI | TTCGAA | 1 cut(s) | 44 |
| BstC8I | GCNNGC | 2 cut(s) | 254, 1035 |
| BstDEI | CTNAG | 8 cut(s) | 474, 525, 710, 743, 1259, 1422, 1493, 1588 |
| BstDSI | CCRYGG | 1 cut(s) | 959 |
| BstF5I | GGATG | 5 cut(s) | 122, 823, 1054, 1722, 1882 |
| BstFNI | CGCG | 2 cut(s) | 736, 1787 |
| BstHHI | GCGC | 1 cut(s) | 385 |
| BstKTI | GATC | 8 cut(s) | 16, 123, 198, 295, 787, 1108, 1489, 1564 |
| BstMAI | GTCTC | 4 cut(s) | 335, 404, 1686, 1700 |
| BstMBI | GATC | 8 cut(s) | 13, 120, 195, 292, 784, 1105, 1486, 1561 |
| BstMCI | CGRYCG | 1 cut(s) | 328 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 391, 1039, 1163, 1172, 1503, 1754 |
| BstNI | CCWGG | 3 cut(s) | 204, 723, 1296 |
| BstNSI | RCATGY | 1 cut(s) | 458 |
| BstSCI | CCNGG | 5 cut(s) | 202, 721, 1152, 1294, 1312 |
| BstSFI | CTRYAG | 1 cut(s) | 1478 |
| BstUI | CGCG | 2 cut(s) | 736, 1787 |
| BstV1I | GCAGC | 8 cut(s) | 164, 470, 541, 607, 853, 986, 1521, 1607 |
| BstV2I | GAAGAC | 3 cut(s) | 158, 765, 1662 |
| BstX2I | RGATCY | 1 cut(s) | 1486 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 305 |
| BstYI | RGATCY | 1 cut(s) | 1486 |
| BstZI | CGGCCG | 1 cut(s) | 325 |
| BsuI | GTATCC | 2 cut(s) | 131, 855 |
| BsuRI | GGCC | 5 cut(s) | 327, 780, 791, 948, 984 |
| BtgI | CCRYGG | 1 cut(s) | 959 |
| BtgZI | GCGATG | 1 cut(s) | 1250 |
| BtrI | CACGTC | 2 cut(s) | 1402, 1889 |
| BtsCI | GGATG | 5 cut(s) | 122, 823, 1054, 1722, 1882 |
| BtsI | GCAGTG | 1 cut(s) | 1282 |
| BtsIMutI | CAGTG | 5 cut(s) | 76, 130, 718, 1282, 1797 |
| BveI | ACCTGC | 2 cut(s) | 589, 622 |
| Cac8I | GCNNGC | 2 cut(s) | 254, 1035 |
| CaiI | CAGNNNCTG | 1 cut(s) | 1737 |
| CfoI | GCGC | 1 cut(s) | 385 |
| Cfr13I | GGNCC | 4 cut(s) | 276, 790, 871, 947 |
| CseI | GACGC | 2 cut(s) | 911, 1776 |
| Csp6I | GTAC | 5 cut(s) | 581, 590, 750, 910, 1907 |
| CviQI | GTAC | 5 cut(s) | 581, 590, 750, 910, 1907 |
| DdeI | CTNAG | 8 cut(s) | 474, 525, 710, 743, 1259, 1422, 1493, 1588 |
| DpnI | GATC | 8 cut(s) | 15, 122, 197, 294, 786, 1107, 1488, 1563 |
| DpnII | GATC | 8 cut(s) | 13, 120, 195, 292, 784, 1105, 1486, 1561 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 1786 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 1786 |
| EaeI | YGGCCR | 3 cut(s) | 325, 778, 982 |
| EagI | CGGCCG | 1 cut(s) | 325 |
| EciI | GGCGGA | 1 cut(s) | 1564 |
| Ecl136II | GAGCTC | 2 cut(s) | 74, 1166 |
| EclXI | CGGCCG | 1 cut(s) | 325 |
| Eco130I | CCWWGG | 1 cut(s) | 959 |
| Eco24I | GRGCYC | 3 cut(s) | 76, 1168, 1423 |
| Eco31I | GGTCTC | 1 cut(s) | 404 |
| Eco47I | GGWCC | 2 cut(s) | 276, 871 |
| Eco52I | CGGCCG | 1 cut(s) | 325 |
| Eco53kI | GAGCTC | 2 cut(s) | 74, 1166 |
| Eco57I | CTGAAG | 1 cut(s) | 785 |
| Eco72I | CACGTG | 1 cut(s) | 980 |
| Eco88I | CYCGRG | 1 cut(s) | 1683 |
| EcoICRI | GAGCTC | 2 cut(s) | 74, 1166 |
| EcoRI | GAATTC | 2 cut(s) | 965, 1349 |
| EcoRII | CCWGG | 3 cut(s) | 202, 721, 1294 |
| EcoT14I | CCWWGG | 1 cut(s) | 959 |
| EcoT22I | ATGCAT | 3 cut(s) | 6, 1098, 1508 |
| EcoT38I | GRGCYC | 3 cut(s) | 76, 1168, 1423 |
| ErhI | CCWWGG | 1 cut(s) | 959 |
| Esp3I | CGTCTC | 1 cut(s) | 335 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 502, 534 |
| FauI | CCCGC | 1 cut(s) | 387 |
| FokI | GGATG | 5 cut(s) | 129, 810, 1041, 1729, 1889 |
| FriOI | GRGCYC | 3 cut(s) | 76, 1168, 1423 |
| FspAI | RTGCGCAY | 1 cut(s) | 384 |
| FspBI | CTAG | 2 cut(s) | 413, 1652 |
| FspI | TGCGCA | 1 cut(s) | 384 |
| GlaI | GCGC | 1 cut(s) | 384 |
| GsaI | CCCAGC | 1 cut(s) | 1420 |
| GsuI | CTGGAG | 2 cut(s) | 1209, 1387 |
| HaeIII | GGCC | 5 cut(s) | 327, 780, 791, 948, 984 |
| HapII | CCGG | 4 cut(s) | 848, 1058, 1153, 1314 |
| HgaI | GACGC | 2 cut(s) | 911, 1776 |
| HhaI | GCGC | 1 cut(s) | 385 |
| Hin6I | GCGC | 1 cut(s) | 383 |
| HinP1I | GCGC | 1 cut(s) | 383 |
| HindIII | AAGCTT | 1 cut(s) | 1040 |
| HinfI | GANTC | 6 cut(s) | 409, 532, 886, 919, 1675, 1869 |
| HpaII | CCGG | 4 cut(s) | 848, 1058, 1153, 1314 |
| HphI | GGTGA | 4 cut(s) | 110, 287, 698, 1302 |
| Hpy166II | GTNNAC | 2 cut(s) | 697, 716 |
| Hpy188I | TCNGA | 4 cut(s) | 507, 1591, 1696, 1809 |
| Hpy8I | GTNNAC | 2 cut(s) | 697, 716 |
| HpyAV | CCTTC | 5 cut(s) | 571, 819, 884, 1317, 1455 |
| HpyCH4III | ACNGT | 4 cut(s) | 685, 701, 1343, 1783 |
| HpyCH4IV | ACGT | 3 cut(s) | 979, 1401, 1888 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 391, 1039, 1163, 1172, 1503, 1754 |
| HpyF3I | CTNAG | 8 cut(s) | 474, 525, 710, 743, 1259, 1422, 1493, 1588 |
| HpySE526I | ACGT | 3 cut(s) | 979, 1401, 1888 |
| HspAI | GCGC | 1 cut(s) | 383 |
| Kpn2I | TCCGGA | 1 cut(s) | 1057 |
| Kzo9I | GATC | 8 cut(s) | 13, 120, 195, 292, 784, 1105, 1486, 1561 |
| Lsp1109I | GCAGC | 8 cut(s) | 164, 470, 541, 607, 853, 986, 1521, 1607 |
| LweI | GCATC | 6 cut(s) | 370, 916, 1273, 1515, 1546, 1582 |
| MaeI | CTAG | 2 cut(s) | 413, 1652 |
| MaeII | ACGT | 3 cut(s) | 979, 1401, 1888 |
| MaeIII | GTNAC | 6 cut(s) | 98, 1022, 1067, 1229, 1639, 1889 |
| MalI | GATC | 8 cut(s) | 15, 122, 197, 294, 786, 1107, 1488, 1563 |
| MboI | GATC | 8 cut(s) | 13, 120, 195, 292, 784, 1105, 1486, 1561 |
| MflI | RGATCY | 1 cut(s) | 1486 |
| MhlI | GDGCHC | 4 cut(s) | 76, 1035, 1168, 1423 |
| MlsI | TGGCCA | 2 cut(s) | 780, 984 |
| MluNI | TGGCCA | 2 cut(s) | 780, 984 |
| MlyI | GAGTC | 1 cut(s) | 526 |
| MmeI | TCCRAC | 5 cut(s) | 667, 1190, 1339, 1602, 1787 |
| Mox20I | TGGCCA | 2 cut(s) | 780, 984 |
| Mph1103I | ATGCAT | 3 cut(s) | 6, 1098, 1508 |
| MroI | TCCGGA | 1 cut(s) | 1057 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1855 |
| MscI | TGGCCA | 2 cut(s) | 780, 984 |
| MseI | TTAA | 6 cut(s) | 363, 423, 830, 855, 1845, 1881 |
| MslI | CAYNNNNRTG | 5 cut(s) | 9, 34, 303, 711, 1101 |
| Msp20I | TGGCCA | 2 cut(s) | 780, 984 |
| MspA1I | CMGCKG | 3 cut(s) | 547, 747, 866 |
| MspI | CCGG | 4 cut(s) | 848, 1058, 1153, 1314 |
| MspR9I | CCNGG | 5 cut(s) | 204, 723, 1154, 1296, 1314 |
| Mva1269I | GAATGC | 3 cut(s) | 662, 1508, 1757 |
| MvaI | CCWGG | 3 cut(s) | 204, 723, 1296 |
| MvnI | CGCG | 2 cut(s) | 736, 1787 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 391, 1039, 1163, 1172, 1503, 1754 |
| NciI | CCSGG | 2 cut(s) | 1154, 1314 |
| NcoI | CCATGG | 1 cut(s) | 959 |
| NdeII | GATC | 8 cut(s) | 13, 120, 195, 292, 784, 1105, 1486, 1561 |
| NlaIV | GGNNCC | 3 cut(s) | 30, 375, 792 |
| NmuCI | GTSAC | 4 cut(s) | 98, 1022, 1067, 1889 |
| NsbI | TGCGCA | 1 cut(s) | 384 |
| NsiI | ATGCAT | 3 cut(s) | 6, 1098, 1508 |
| NspI | RCATGY | 1 cut(s) | 458 |
| NspV | TTCGAA | 1 cut(s) | 44 |
| OliI | CACNNNNGTG | 2 cut(s) | 34, 711 |
| PctI | GAATGC | 3 cut(s) | 662, 1508, 1757 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1855 |
| PfeI | GAWTC | 5 cut(s) | 409, 886, 919, 1675, 1869 |
| PflFI | GACNNNGTC | 1 cut(s) | 1403 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 787 |
| PfoI | TCCNGGA | 1 cut(s) | 202 |
| PleI | GAGTC | 1 cut(s) | 526 |
| PmaCI | CACGTG | 1 cut(s) | 980 |
| PmlI | CACGTG | 1 cut(s) | 980 |
| PpsI | GAGTC | 1 cut(s) | 526 |
| Ppu21I | YACGTR | 1 cut(s) | 980 |
| Psp124BI | GAGCTC | 2 cut(s) | 76, 1168 |
| Psp6I | CCWGG | 3 cut(s) | 202, 721, 1294 |
| PspCI | CACGTG | 1 cut(s) | 980 |
| PspFI | CCCAGC | 1 cut(s) | 1416 |
| PspGI | CCWGG | 3 cut(s) | 202, 721, 1294 |
| PspN4I | GGNNCC | 3 cut(s) | 30, 375, 792 |
| PspPI | GGNCC | 4 cut(s) | 276, 790, 871, 947 |
| PsrI | GAACNNNNNNTAC | 2 cut(s) | 1632, 1664 |
| PstI | CTGCAG | 1 cut(s) | 1482 |
| PstNI | CAGNNNCTG | 1 cut(s) | 1737 |
| PsuI | RGATCY | 1 cut(s) | 1486 |
| PsyI | GACNNNGTC | 1 cut(s) | 1403 |
| PvuII | CAGCTG | 3 cut(s) | 547, 747, 866 |
| RsaI | GTAC | 5 cut(s) | 582, 591, 751, 911, 1908 |
| RsaNI | GTAC | 5 cut(s) | 581, 590, 750, 910, 1907 |
| RseI | CAYNNNNRTG | 5 cut(s) | 9, 34, 303, 711, 1101 |
| SacI | GAGCTC | 2 cut(s) | 76, 1168 |
| SaqAI | TTAA | 6 cut(s) | 363, 423, 830, 855, 1845, 1881 |
| Sau3AI | GATC | 8 cut(s) | 13, 120, 195, 292, 784, 1105, 1486, 1561 |
| Sau96I | GGNCC | 4 cut(s) | 276, 790, 871, 947 |
| SchI | GAGTC | 1 cut(s) | 526 |
| ScrFI | CCNGG | 5 cut(s) | 204, 723, 1154, 1296, 1314 |
| SduI | GDGCHC | 4 cut(s) | 76, 1035, 1168, 1423 |
| SfaNI | GCATC | 6 cut(s) | 370, 916, 1273, 1515, 1546, 1582 |
| SfcI | CTRYAG | 1 cut(s) | 1478 |
| SfuI | TTCGAA | 1 cut(s) | 44 |
| SinI | GGWCC | 2 cut(s) | 276, 871 |
| SmiMI | CAYNNNNRTG | 5 cut(s) | 9, 34, 303, 711, 1101 |
| SsiI | CCGC | 6 cut(s) | 114, 324, 394, 869, 1244, 1549 |
| SspI | AATATT | 1 cut(s) | 1183 |
| SspMI | CTAG | 2 cut(s) | 413, 1652 |
| SstI | GAGCTC | 2 cut(s) | 76, 1168 |
| StyD4I | CCNGG | 5 cut(s) | 202, 721, 1152, 1294, 1312 |
| StyI | CCWWGG | 1 cut(s) | 959 |
| TaaI | ACNGT | 4 cut(s) | 685, 701, 1343, 1783 |
| TaiI | ACGT | 3 cut(s) | 982, 1404, 1891 |
| TaqI | TCGA | 5 cut(s) | 19, 44, 535, 672, 1616 |
| TatI | WGTACW | 3 cut(s) | 589, 749, 909 |
| TauI | GCSGC | 2 cut(s) | 327, 1247 |
| TfiI | GAWTC | 5 cut(s) | 409, 886, 919, 1675, 1869 |
| Tru1I | TTAA | 6 cut(s) | 363, 423, 830, 855, 1845, 1881 |
| Tru9I | TTAA | 6 cut(s) | 363, 423, 830, 855, 1845, 1881 |
| TscAI | CASTG | 5 cut(s) | 83, 130, 718, 1282, 1797 |
| TseFI | GTSAC | 4 cut(s) | 98, 1022, 1067, 1889 |
| TseI | GCWGC | 8 cut(s) | 152, 458, 554, 595, 866, 974, 1534, 1595 |
| Tsp45I | GTSAC | 4 cut(s) | 98, 1022, 1067, 1889 |
| TspDTI | ATGAA | 9 cut(s) | 315, 624, 771, 866, 1034, 1191, 1536, 1649, 1861 |
| TspRI | CASTG | 5 cut(s) | 83, 130, 718, 1282, 1797 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1403 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 787 |
| VpaK11BI | GGWCC | 2 cut(s) | 276, 871 |
| XapI | RAATTY | 4 cut(s) | 965, 1349, 1622, 1855 |
| XbaI | TCTAGA | 2 cut(s) | 412, 1651 |
| XceI | RCATGY | 1 cut(s) | 458 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 957 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1855 |
| XspI | CTAG | 2 cut(s) | 413, 1652 |
| Zsp2I | ATGCAT | 3 cut(s) | 6, 1098, 1508 |