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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 4 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1020 |
| AccI | GTMKAC | 2 cut(s) | 353, 2157 |
| AccIII | TCCGGA | 2 cut(s) | 1348, 2464 |
| AciI | CCGC | 4 cut(s) | 52, 439, 730, 1079 |
| AclI | AACGTT | 1 cut(s) | 34 |
| AclWI | GGATC | 5 cut(s) | 493, 506, 673, 1129, 1548 |
| AcoI | YGGCCR | 2 cut(s) | 1080, 2020 |
| AcsI | RAATTY | 4 cut(s) | 872, 1618, 2190, 2343 |
| AcuI | CTGAAG | 3 cut(s) | 90, 1155, 2029 |
| AdeI | CACNNNGTG | 1 cut(s) | 1020 |
| AfaI | GTAC | 7 cut(s) | 569, 589, 768, 923, 1473, 1722, 2430 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 319, 1020, 1137, 2202 |
| AflIII | ACRYGT | 1 cut(s) | 700 |
| AhlI | ACTAGT | 1 cut(s) | 263 |
| AjnI | CCWGG | 4 cut(s) | 1183, 1781, 1905, 2242 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 2330, 2362 |
| Alw21I | GWGCWC | 2 cut(s) | 133, 1955 |
| Alw26I | GTCTC | 4 cut(s) | 172, 1625, 1886, 2211 |
| Alw44I | GTGCAC | 1 cut(s) | 1951 |
| AlwI | GGATC | 5 cut(s) | 493, 506, 673, 1129, 1548 |
| Ama87I | CYCGRG | 1 cut(s) | 1064 |
| Aor13HI | TCCGGA | 2 cut(s) | 1348, 2464 |
| AoxI | GGCC | 6 cut(s) | 339, 669, 1080, 1309, 1784, 2020 |
| ApaLI | GTGCAC | 1 cut(s) | 1951 |
| ApoI | RAATTY | 4 cut(s) | 872, 1618, 2190, 2343 |
| AspLEI | GCGC | 2 cut(s) | 2285, 2331 |
| AspS9I | GGNCC | 3 cut(s) | 542, 595, 1351 |
| AsuHPI | GGTGA | 7 cut(s) | 472, 920, 1053, 1147, 1209, 1418, 1721 |
| AvaI | CYCGRG | 1 cut(s) | 1064 |
| AvaII | GGWCC | 3 cut(s) | 542, 595, 1351 |
| AxyI | CCTNAGG | 2 cut(s) | 220, 1788 |
| BaeGI | GKGCMC | 1 cut(s) | 1955 |
| BamHI | GGATCC | 1 cut(s) | 498 |
| BanI | GGYRCC | 1 cut(s) | 4 |
| BbsI | GAAGAC | 1 cut(s) | 1991 |
| Bbv12I | GWGCWC | 2 cut(s) | 133, 1955 |
| BbvCI | CCTCAGC | 1 cut(s) | 1116 |
| BccI | CCATC | 7 cut(s) | 16, 119, 1040, 1228, 1397, 2128, 2294 |
| BceAI | ACGGC | 2 cut(s) | 252, 2007 |
| BciT130I | CCWGG | 4 cut(s) | 1185, 1783, 1907, 2244 |
| BclI | TGATCA | 1 cut(s) | 489 |
| BcoDI | GTCTC | 4 cut(s) | 172, 1625, 1886, 2211 |
| BcuI | ACTAGT | 1 cut(s) | 263 |
| BfaI | CTAG | 3 cut(s) | 264, 450, 1508 |
| BfmI | CTRYAG | 3 cut(s) | 1224, 1809, 2456 |
| BfoI | RGCGCY | 1 cut(s) | 2286 |
| BglII | AGATCT | 1 cut(s) | 2250 |
| BlpI | GCTNAGC | 2 cut(s) | 48, 273 |
| Bme1390I | CCNGG | 4 cut(s) | 1185, 1783, 1907, 2244 |
| Bme18I | GGWCC | 3 cut(s) | 542, 595, 1351 |
| BmeT110I | CYCGRG | 1 cut(s) | 1064 |
| BmgT120I | GGNCC | 3 cut(s) | 542, 595, 1351 |
| BmiI | GGNNCC | 5 cut(s) | 6, 500, 597, 2095, 2386 |
| BmrFI | CCNGG | 4 cut(s) | 1185, 1783, 1907, 2244 |
| BmrI | ACTGGG | 3 cut(s) | 343, 438, 2283 |
| BmsI | GCATC | 8 cut(s) | 29, 202, 809, 831, 990, 1189, 1702, 1750 |
| BmuI | ACTGGG | 3 cut(s) | 343, 438, 2283 |
| BpiI | GAAGAC | 1 cut(s) | 1991 |
| BpmI | CTGGAG | 2 cut(s) | 117, 789 |
| Bpu10I | CCTNAGC | 2 cut(s) | 1116, 1159 |
| Bpu1102I | GCTNAGC | 2 cut(s) | 48, 273 |
| Bsa29I | ATCGAT | 1 cut(s) | 78 |
| BsaAI | YACGTR | 3 cut(s) | 587, 676, 941 |
| BsaI | GGTCTC | 1 cut(s) | 2211 |
| BsaJI | CCNNGG | 7 cut(s) | 234, 314, 526, 1065, 1312, 1460, 2243 |
| BsaWI | WCCGGW | 2 cut(s) | 1348, 2464 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 1866, 1896, 2129, 2159 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 319, 1020, 1137, 2202 |
| Bse1I | ACTGG | 5 cut(s) | 349, 433, 536, 1730, 2278 |
| Bse21I | CCTNAGG | 2 cut(s) | 220, 1788 |
| BseAI | TCCGGA | 2 cut(s) | 1348, 2464 |
| BseBI | CCWGG | 4 cut(s) | 1185, 1783, 1907, 2244 |
| BseCI | ATCGAT | 1 cut(s) | 78 |
| BseDI | CCNNGG | 7 cut(s) | 234, 314, 526, 1065, 1312, 1460, 2243 |
| BseGI | GGATG | 6 cut(s) | 130, 217, 634, 822, 1051, 1180 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 319, 1020, 1137, 2202 |
| BseMII | CTCAG | 5 cut(s) | 234, 264, 777, 1107, 1283 |
| BseNI | ACTGG | 5 cut(s) | 349, 433, 536, 1730, 2278 |
| BseRI | GAGGAG | 3 cut(s) | 680, 1212, 2448 |
| BseSI | GKGCMC | 1 cut(s) | 1955 |
| BseYI | CCCAGC | 1 cut(s) | 982 |
| BsgI | GTGCAG | 1 cut(s) | 215 |
| Bsh1285I | CGRYCG | 1 cut(s) | 1009 |
| BshFI | GGCC | 6 cut(s) | 341, 671, 1082, 1311, 1786, 2022 |
| BshNI | GGYRCC | 1 cut(s) | 4 |
| BshVI | ATCGAT | 1 cut(s) | 78 |
| BsiEI | CGRYCG | 1 cut(s) | 1009 |
| BsiHKAI | GWGCWC | 2 cut(s) | 133, 1955 |
| BsiHKCI | CYCGRG | 1 cut(s) | 1064 |
| BsiSI | CCGG | 2 cut(s) | 1349, 2465 |
| BsiWI | CGTACG | 1 cut(s) | 587 |
| BslFI | GGGAC | 4 cut(s) | 331, 544, 581, 974 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 319, 1020, 1137, 2202 |
| BsmAI | GTCTC | 4 cut(s) | 172, 1625, 1886, 2211 |
| BsmBI | CGTCTC | 1 cut(s) | 172 |
| BsmFI | GGGAC | 4 cut(s) | 331, 544, 581, 974 |
| BsmI | GAATGC | 4 cut(s) | 166, 259, 376, 1638 |
| BsnI | GGCC | 6 cut(s) | 341, 671, 1082, 1311, 1786, 2022 |
| Bso31I | GGTCTC | 1 cut(s) | 2211 |
| BsoBI | CYCGRG | 1 cut(s) | 1064 |
| Bsp1286I | GDGCHC | 2 cut(s) | 133, 1955 |
| Bsp13I | TCCGGA | 2 cut(s) | 1348, 2464 |
| Bsp1407I | TGTACA | 1 cut(s) | 1471 |
| Bsp143I | GATC | 9 cut(s) | 489, 498, 678, 1006, 1121, 1540, 2164, 2212, 2250 |
| Bsp1720I | GCTNAGC | 2 cut(s) | 48, 273 |
| Bsp19I | CCATGG | 2 cut(s) | 526, 1312 |
| BspACI | CCGC | 4 cut(s) | 52, 439, 730, 1079 |
| BspANI | GGCC | 6 cut(s) | 341, 671, 1082, 1311, 1786, 2022 |
| BspCNI | CTCAG | 5 cut(s) | 233, 265, 778, 1108, 1282 |
| BspDI | ATCGAT | 1 cut(s) | 78 |
| BspEI | TCCGGA | 2 cut(s) | 1348, 2464 |
| BspHI | TCATGA | 2 cut(s) | 141, 1614 |
| BspLI | GGNNCC | 5 cut(s) | 6, 500, 597, 2095, 2386 |
| BspMAI | CTGCAG | 2 cut(s) | 1813, 2460 |
| BspPI | GGATC | 5 cut(s) | 493, 506, 673, 1129, 1548 |
| BspT107I | GGYRCC | 1 cut(s) | 4 |
| BspTNI | GGTCTC | 1 cut(s) | 2211 |
| BsrGI | TGTACA | 1 cut(s) | 1471 |
| BsrI | ACTGG | 5 cut(s) | 349, 433, 536, 1730, 2278 |
| BssECI | CCNNGG | 7 cut(s) | 234, 314, 526, 1065, 1312, 1460, 2243 |
| BssMI | GATC | 9 cut(s) | 489, 498, 678, 1006, 1121, 1540, 2164, 2212, 2250 |
| BssT1I | CCWWGG | 3 cut(s) | 314, 526, 1312 |
| Bst2UI | CCWGG | 4 cut(s) | 1185, 1783, 1907, 2244 |
| Bst4CI | ACNGT | 9 cut(s) | 304, 595, 932, 1381, 1521, 1585, 1690, 1753, 2162 |
| Bst6I | CTCTTC | 1 cut(s) | 1554 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 1820 |
| BstAUI | TGTACA | 1 cut(s) | 1471 |
| BstBAI | YACGTR | 3 cut(s) | 587, 676, 941 |
| BstC8I | GCNNGC | 2 cut(s) | 1026, 2240 |
| BstDSI | CCRYGG | 3 cut(s) | 234, 526, 1312 |
| BstF5I | GGATG | 6 cut(s) | 130, 217, 634, 822, 1051, 1180 |
| BstH2I | RGCGCY | 1 cut(s) | 2286 |
| BstHHI | GCGC | 2 cut(s) | 2285, 2331 |
| BstKTI | GATC | 9 cut(s) | 492, 501, 681, 1009, 1124, 1543, 2167, 2215, 2253 |
| BstMAI | GTCTC | 4 cut(s) | 172, 1625, 1886, 2211 |
| BstMBI | GATC | 9 cut(s) | 489, 498, 678, 1006, 1121, 1540, 2164, 2212, 2250 |
| BstMCI | CGRYCG | 1 cut(s) | 1009 |
| BstMWI | GCNNNNNNNGC | 8 cut(s) | 65, 106, 224, 1317, 1817, 1820, 1826, 1829 |
| BstNI | CCWGG | 4 cut(s) | 1185, 1783, 1907, 2244 |
| BstNSI | RCATGY | 1 cut(s) | 704 |
| BstSCI | CCNGG | 4 cut(s) | 1183, 1781, 1905, 2242 |
| BstSFI | CTRYAG | 3 cut(s) | 1224, 1809, 2456 |
| BstSLI | GKGCMC | 1 cut(s) | 1955 |
| BstV2I | GAAGAC | 1 cut(s) | 1991 |
| BstX2I | RGATCY | 3 cut(s) | 498, 1540, 2250 |
| BstYI | RGATCY | 3 cut(s) | 498, 1540, 2250 |
| Bsu15I | ATCGAT | 1 cut(s) | 78 |
| Bsu36I | CCTNAGG | 2 cut(s) | 220, 1788 |
| BsuRI | GGCC | 6 cut(s) | 341, 671, 1082, 1311, 1786, 2022 |
| BsuTUI | ATCGAT | 1 cut(s) | 78 |
| BtgI | CCRYGG | 3 cut(s) | 234, 526, 1312 |
| BtsCI | GGATG | 6 cut(s) | 130, 217, 634, 822, 1051, 1180 |
| BtsIMutI | CAGTG | 4 cut(s) | 1131, 1737, 2179, 2271 |
| Cac8I | GCNNGC | 2 cut(s) | 1026, 2240 |
| CciI | TCATGA | 2 cut(s) | 141, 1614 |
| CfoI | GCGC | 2 cut(s) | 2285, 2331 |
| Cfr13I | GGNCC | 3 cut(s) | 542, 595, 1351 |
| ClaI | ATCGAT | 1 cut(s) | 78 |
| CseI | GACGC | 1 cut(s) | 875 |
| Csp6I | GTAC | 7 cut(s) | 568, 588, 767, 922, 1472, 1721, 2429 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 1920, 1955 |
| CviQI | GTAC | 7 cut(s) | 568, 588, 767, 922, 1472, 1721, 2429 |
| DpnI | GATC | 9 cut(s) | 491, 500, 680, 1008, 1123, 1542, 2166, 2214, 2252 |
| DpnII | GATC | 9 cut(s) | 489, 498, 678, 1006, 1121, 1540, 2164, 2212, 2250 |
| DraI | TTTAAA | 1 cut(s) | 2076 |
| DraIII | CACNNNGTG | 1 cut(s) | 1020 |
| EaeI | YGGCCR | 2 cut(s) | 1080, 2020 |
| Eam1104I | CTCTTC | 1 cut(s) | 1554 |
| EarI | CTCTTC | 1 cut(s) | 1554 |
| Eco130I | CCWWGG | 3 cut(s) | 314, 526, 1312 |
| Eco147I | AGGCCT | 1 cut(s) | 671 |
| Eco31I | GGTCTC | 1 cut(s) | 2211 |
| Eco47I | GGWCC | 3 cut(s) | 542, 595, 1351 |
| Eco57I | CTGAAG | 3 cut(s) | 90, 1155, 2029 |
| Eco81I | CCTNAGG | 2 cut(s) | 220, 1788 |
| Eco88I | CYCGRG | 1 cut(s) | 1064 |
| EcoRI | GAATTC | 1 cut(s) | 2190 |
| EcoRII | CCWGG | 4 cut(s) | 1183, 1781, 1905, 2242 |
| EcoT14I | CCWWGG | 3 cut(s) | 314, 526, 1312 |
| EcoT22I | ATGCAT | 1 cut(s) | 824 |
| ErhI | CCWWGG | 3 cut(s) | 314, 526, 1312 |
| Esp3I | CGTCTC | 1 cut(s) | 172 |
| FaqI | GGGAC | 4 cut(s) | 331, 544, 581, 974 |
| FauI | CCCGC | 1 cut(s) | 723 |
| FauNDI | CATATG | 1 cut(s) | 102 |
| FbaI | TGATCA | 1 cut(s) | 489 |
| FblI | GTMKAC | 2 cut(s) | 353, 2157 |
| FokI | GGATG | 6 cut(s) | 137, 224, 641, 809, 1058, 1167 |
| FspBI | CTAG | 3 cut(s) | 264, 450, 1508 |
| GlaI | GCGC | 2 cut(s) | 2284, 2330 |
| GsaI | CCCAGC | 1 cut(s) | 986 |
| GsuI | CTGGAG | 2 cut(s) | 117, 789 |
| HaeII | RGCGCY | 1 cut(s) | 2286 |
| HaeIII | GGCC | 6 cut(s) | 341, 671, 1082, 1311, 1786, 2022 |
| HapII | CCGG | 2 cut(s) | 1349, 2465 |
| HgaI | GACGC | 1 cut(s) | 875 |
| HhaI | GCGC | 2 cut(s) | 2285, 2331 |
| Hin6I | GCGC | 2 cut(s) | 2283, 2329 |
| HinP1I | GCGC | 2 cut(s) | 2283, 2329 |
| HincII | GTYRAC | 3 cut(s) | 1143, 1588, 2158 |
| HindII | GTYRAC | 3 cut(s) | 1143, 1588, 2158 |
| HindIII | AAGCTT | 1 cut(s) | 1340 |
| HinfI | GANTC | 9 cut(s) | 183, 286, 576, 656, 735, 987, 1611, 1726, 2260 |
| HpaII | CCGG | 2 cut(s) | 1349, 2465 |
| HphI | GGTGA | 7 cut(s) | 472, 920, 1053, 1147, 1209, 1418, 1721 |
| Hpy166II | GTNNAC | 8 cut(s) | 354, 1143, 1457, 1483, 1588, 1953, 2059, 2158 |
| Hpy188I | TCNGA | 7 cut(s) | 31, 124, 494, 1272, 1545, 2048, 2400 |
| Hpy8I | GTNNAC | 8 cut(s) | 354, 1143, 1457, 1483, 1588, 1953, 2059, 2158 |
| Hpy99I | CGWCG | 1 cut(s) | 595 |
| HpyAV | CCTTC | 8 cut(s) | 37, 313, 1069, 1130, 1340, 1364, 1700, 1936 |
| HpyCH4III | ACNGT | 9 cut(s) | 304, 595, 932, 1381, 1521, 1585, 1690, 1753, 2162 |
| HpyCH4IV | ACGT | 5 cut(s) | 34, 586, 675, 940, 1365 |
| HpyF10VI | GCNNNNNNNGC | 8 cut(s) | 65, 106, 224, 1317, 1817, 1820, 1826, 1829 |
| HpySE526I | ACGT | 5 cut(s) | 34, 586, 675, 940, 1365 |
| HspAI | GCGC | 2 cut(s) | 2283, 2329 |
| Kpn2I | TCCGGA | 2 cut(s) | 1348, 2464 |
| Ksp22I | TGATCA | 1 cut(s) | 489 |
| Kzo9I | GATC | 9 cut(s) | 489, 498, 678, 1006, 1121, 1540, 2164, 2212, 2250 |
| LmnI | GCTCC | 6 cut(s) | 136, 224, 837, 1317, 2093, 2290 |
| LweI | GCATC | 8 cut(s) | 29, 202, 809, 831, 990, 1189, 1702, 1750 |
| MaeI | CTAG | 3 cut(s) | 264, 450, 1508 |
| MaeII | ACGT | 5 cut(s) | 34, 586, 675, 940, 1365 |
| MaeIII | GTNAC | 8 cut(s) | 460, 475, 840, 936, 1282, 1416, 1684, 1801 |
| MalI | GATC | 9 cut(s) | 491, 500, 680, 1008, 1123, 1542, 2166, 2214, 2252 |
| MboI | GATC | 9 cut(s) | 489, 498, 678, 1006, 1121, 1540, 2164, 2212, 2250 |
| MboII | GAAGA | 9 cut(s) | 169, 866, 1180, 1399, 1571, 1855, 1996, 2035, 2134 |
| MfeI | CAATTG | 3 cut(s) | 660, 1764, 2178 |
| MflI | RGATCY | 3 cut(s) | 498, 1540, 2250 |
| MhlI | GDGCHC | 2 cut(s) | 133, 1955 |
| MlyI | GAGTC | 3 cut(s) | 585, 996, 1620 |
| MmeI | TCCRAC | 2 cut(s) | 526, 774 |
| Mph1103I | ATGCAT | 1 cut(s) | 824 |
| MroI | TCCGGA | 2 cut(s) | 1348, 2464 |
| MseI | TTAA | 6 cut(s) | 83, 90, 876, 1100, 1344, 2075 |
| MslI | CAYNNNNRTG | 3 cut(s) | 1289, 1680, 2114 |
| MspI | CCGG | 2 cut(s) | 1349, 2465 |
| MspR9I | CCNGG | 4 cut(s) | 1185, 1783, 1907, 2244 |
| MunI | CAATTG | 3 cut(s) | 660, 1764, 2178 |
| Mva1269I | GAATGC | 4 cut(s) | 166, 259, 376, 1638 |
| MvaI | CCWGG | 4 cut(s) | 1185, 1783, 1907, 2244 |
| MwoI | GCNNNNNNNGC | 8 cut(s) | 65, 106, 224, 1317, 1817, 1820, 1826, 1829 |
| NcoI | CCATGG | 2 cut(s) | 526, 1312 |
| NdeI | CATATG | 1 cut(s) | 102 |
| NdeII | GATC | 9 cut(s) | 489, 498, 678, 1006, 1121, 1540, 2164, 2212, 2250 |
| NlaIV | GGNNCC | 5 cut(s) | 6, 500, 597, 2095, 2386 |
| NmuCI | GTSAC | 5 cut(s) | 460, 840, 936, 1282, 1684 |
| NsiI | ATGCAT | 1 cut(s) | 824 |
| NspI | RCATGY | 1 cut(s) | 704 |
| PagI | TCATGA | 2 cut(s) | 141, 1614 |
| PceI | AGGCCT | 1 cut(s) | 671 |
| PciI | ACATGT | 1 cut(s) | 700 |
| PctI | GAATGC | 4 cut(s) | 166, 259, 376, 1638 |
| PfeI | GAWTC | 6 cut(s) | 183, 286, 656, 735, 1726, 2260 |
| Pfl23II | CGTACG | 1 cut(s) | 587 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1020 |
| Ple19I | CGATCG | 1 cut(s) | 1009 |
| PleI | GAGTC | 3 cut(s) | 584, 995, 1619 |
| PpsI | GAGTC | 3 cut(s) | 584, 995, 1619 |
| Ppu21I | YACGTR | 3 cut(s) | 587, 676, 941 |
| PscI | ACATGT | 1 cut(s) | 700 |
| Psp1406I | AACGTT | 1 cut(s) | 34 |
| Psp6I | CCWGG | 4 cut(s) | 1183, 1781, 1905, 2242 |
| PspFI | CCCAGC | 1 cut(s) | 982 |
| PspGI | CCWGG | 4 cut(s) | 1183, 1781, 1905, 2242 |
| PspLI | CGTACG | 1 cut(s) | 587 |
| PspN4I | GGNNCC | 5 cut(s) | 6, 500, 597, 2095, 2386 |
| PspPI | GGNCC | 3 cut(s) | 542, 595, 1351 |
| PstI | CTGCAG | 2 cut(s) | 1813, 2460 |
| PsuI | RGATCY | 3 cut(s) | 498, 1540, 2250 |
| PvuI | CGATCG | 1 cut(s) | 1009 |
| RsaI | GTAC | 7 cut(s) | 569, 589, 768, 923, 1473, 1722, 2430 |
| RsaNI | GTAC | 7 cut(s) | 568, 588, 767, 922, 1472, 1721, 2429 |
| RseI | CAYNNNNRTG | 3 cut(s) | 1289, 1680, 2114 |
| SalI | GTCGAC | 1 cut(s) | 2156 |
| SaqAI | TTAA | 6 cut(s) | 83, 90, 876, 1100, 1344, 2075 |
| Sau3AI | GATC | 9 cut(s) | 489, 498, 678, 1006, 1121, 1540, 2164, 2212, 2250 |
| Sau96I | GGNCC | 3 cut(s) | 542, 595, 1351 |
| SchI | GAGTC | 3 cut(s) | 585, 996, 1620 |
| ScrFI | CCNGG | 4 cut(s) | 1185, 1783, 1907, 2244 |
| SduI | GDGCHC | 2 cut(s) | 133, 1955 |
| SfaNI | GCATC | 8 cut(s) | 29, 202, 809, 831, 990, 1189, 1702, 1750 |
| SfcI | CTRYAG | 3 cut(s) | 1224, 1809, 2456 |
| SinI | GGWCC | 3 cut(s) | 542, 595, 1351 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 1289, 1680, 2114 |
| SpeI | ACTAGT | 1 cut(s) | 263 |
| SseBI | AGGCCT | 1 cut(s) | 671 |
| SsiI | CCGC | 4 cut(s) | 52, 439, 730, 1079 |
| SspI | AATATT | 3 cut(s) | 424, 1098, 1594 |
| SspMI | CTAG | 3 cut(s) | 264, 450, 1508 |
| StuI | AGGCCT | 1 cut(s) | 671 |
| StyD4I | CCNGG | 4 cut(s) | 1183, 1781, 1905, 2242 |
| StyI | CCWWGG | 3 cut(s) | 314, 526, 1312 |
| TaaI | ACNGT | 9 cut(s) | 304, 595, 932, 1381, 1521, 1585, 1690, 1753, 2162 |
| TaiI | ACGT | 5 cut(s) | 37, 589, 678, 943, 1368 |
| TaqI | TCGA | 7 cut(s) | 78, 380, 1005, 1567, 1755, 2087, 2157 |
| TatI | WGTACW | 2 cut(s) | 921, 1471 |
| TauI | GCSGC | 2 cut(s) | 442, 1082 |
| TfiI | GAWTC | 6 cut(s) | 183, 286, 656, 735, 1726, 2260 |
| Tru1I | TTAA | 6 cut(s) | 83, 90, 876, 1100, 1344, 2075 |
| Tru9I | TTAA | 6 cut(s) | 83, 90, 876, 1100, 1344, 2075 |
| TscAI | CASTG | 4 cut(s) | 1138, 1737, 2179, 2278 |
| TseFI | GTSAC | 5 cut(s) | 460, 840, 936, 1282, 1684 |
| Tsp45I | GTSAC | 5 cut(s) | 460, 840, 936, 1282, 1684 |
| TspGWI | ACGGA | 1 cut(s) | 2106 |
| TspRI | CASTG | 4 cut(s) | 1138, 1737, 2179, 2278 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1020 |
| VneI | GTGCAC | 1 cut(s) | 1951 |
| VpaK11BI | GGWCC | 3 cut(s) | 542, 595, 1351 |
| XapI | RAATTY | 4 cut(s) | 872, 1618, 2190, 2343 |
| XbaI | TCTAGA | 1 cut(s) | 1507 |
| XceI | RCATGY | 1 cut(s) | 704 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1021 |
| XmiI | GTMKAC | 2 cut(s) | 353, 2157 |
| XspI | CTAG | 3 cut(s) | 264, 450, 1508 |
| Zsp2I | ATGCAT | 1 cut(s) | 824 |