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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 523 |
| AccII | CGCG | 1 cut(s) | 630 |
| AccIII | TCCGGA | 1 cut(s) | 1218 |
| AciI | CCGC | 1 cut(s) | 1098 |
| AclWI | GGATC | 5 cut(s) | 266, 935, 948, 1133, 1146 |
| AcuI | CTGAAG | 2 cut(s) | 669, 821 |
| AcyI | GRCGYC | 1 cut(s) | 524 |
| AfaI | GTAC | 1 cut(s) | 716 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 115, 367, 390, 577, 950, 1148 |
| AflII | CTTAAG | 1 cut(s) | 184 |
| AgsI | TTSAA | 6 cut(s) | 73, 115, 140, 497, 513, 733 |
| AjnI | CCWGG | 4 cut(s) | 22, 880, 949, 1179 |
| AluBI | AGCT | 6 cut(s) | 99, 332, 336, 689, 830, 1187 |
| AluI | AGCT | 6 cut(s) | 99, 332, 336, 689, 830, 1187 |
| Alw26I | GTCTC | 1 cut(s) | 140 |
| AlwI | GGATC | 5 cut(s) | 266, 935, 948, 1133, 1146 |
| Ama87I | CYCGRG | 2 cut(s) | 14, 1026 |
| Aor13HI | TCCGGA | 1 cut(s) | 1218 |
| AoxI | GGCC | 4 cut(s) | 10, 180, 883, 975 |
| ApaI | GGGCCC | 1 cut(s) | 14 |
| ApeKI | GCWGC | 3 cut(s) | 336, 750, 827 |
| AseI | ATTAAT | 1 cut(s) | 257 |
| AspLEI | GCGC | 3 cut(s) | 526, 630, 1116 |
| AspS9I | GGNCC | 3 cut(s) | 10, 11, 357 |
| AsuHPI | GGTGA | 5 cut(s) | 106, 359, 515, 635, 863 |
| AsuNHI | GCTAGC | 1 cut(s) | 332 |
| AvaI | CYCGRG | 2 cut(s) | 14, 1026 |
| AvaII | GGWCC | 1 cut(s) | 357 |
| AxyI | CCTNAGG | 1 cut(s) | 576 |
| BaeGI | GKGCMC | 1 cut(s) | 14 |
| BamHI | GGATCC | 2 cut(s) | 940, 1138 |
| BanI | GGYRCC | 1 cut(s) | 523 |
| BanII | GRGCYC | 1 cut(s) | 14 |
| BbsI | GAAGAC | 1 cut(s) | 848 |
| BbvI | GCAGC | 3 cut(s) | 323, 762, 839 |
| BccI | CCATC | 3 cut(s) | 151, 184, 535 |
| BceAI | ACGGC | 1 cut(s) | 870 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 553, 587 |
| BciT130I | CCWGG | 4 cut(s) | 24, 882, 951, 1181 |
| BcoDI | GTCTC | 1 cut(s) | 140 |
| BfaI | CTAG | 2 cut(s) | 198, 333 |
| BfmI | CTRYAG | 1 cut(s) | 777 |
| BfoI | RGCGCY | 1 cut(s) | 527 |
| BfrI | CTTAAG | 1 cut(s) | 184 |
| BisI | GCNGC | 3 cut(s) | 337, 751, 828 |
| BlsI | GCNGC | 3 cut(s) | 338, 752, 829 |
| Bme1390I | CCNGG | 4 cut(s) | 24, 882, 951, 1181 |
| Bme18I | GGWCC | 1 cut(s) | 357 |
| BmeT110I | CYCGRG | 2 cut(s) | 14, 1026 |
| BmgT120I | GGNCC | 3 cut(s) | 10, 11, 357 |
| BmiI | GGNNCC | 6 cut(s) | 12, 13, 359, 525, 942, 1140 |
| BmrFI | CCNGG | 4 cut(s) | 24, 882, 951, 1181 |
| BmsI | GCATC | 3 cut(s) | 28, 858, 1101 |
| BmtI | GCTAGC | 1 cut(s) | 336 |
| BpiI | GAAGAC | 1 cut(s) | 848 |
| BpmI | CTGGAG | 1 cut(s) | 363 |
| Bpu10I | CCTNAGC | 1 cut(s) | 165 |
| BsaHI | GRCGYC | 1 cut(s) | 524 |
| BsaJI | CCNNGG | 3 cut(s) | 59, 1146, 1170 |
| BsaWI | WCCGGW | 1 cut(s) | 1218 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 336, 366, 678, 708 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 115, 367, 390, 577, 950, 1148 |
| Bse1I | ACTGG | 1 cut(s) | 825 |
| Bse21I | CCTNAGG | 1 cut(s) | 576 |
| Bse3DI | GCAATG | 1 cut(s) | 1093 |
| BseAI | TCCGGA | 1 cut(s) | 1218 |
| BseBI | CCWGG | 4 cut(s) | 24, 882, 951, 1181 |
| BseDI | CCNNGG | 3 cut(s) | 59, 1146, 1170 |
| BseGI | GGATG | 3 cut(s) | 19, 195, 379 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 115, 367, 390, 577, 950, 1148 |
| BseMI | GCAATG | 1 cut(s) | 1093 |
| BseMII | CTCAG | 4 cut(s) | 213, 230, 567, 1077 |
| BseNI | ACTGG | 1 cut(s) | 825 |
| BseSI | GKGCMC | 1 cut(s) | 14 |
| BseXI | GCAGC | 3 cut(s) | 323, 762, 839 |
| BseYI | CCCAGC | 1 cut(s) | 1082 |
| BsgI | GTGCAG | 1 cut(s) | 769 |
| Bsh1236I | CGCG | 1 cut(s) | 630 |
| Bsh1285I | CGRYCG | 1 cut(s) | 109 |
| BshFI | GGCC | 4 cut(s) | 12, 182, 885, 977 |
| BshNI | GGYRCC | 1 cut(s) | 523 |
| BsiEI | CGRYCG | 1 cut(s) | 109 |
| BsiHKCI | CYCGRG | 2 cut(s) | 14, 1026 |
| BsiSI | CCGG | 1 cut(s) | 1219 |
| BslFI | GGGAC | 2 cut(s) | 343, 1139 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 115, 367, 390, 577, 950, 1148 |
| BsmAI | GTCTC | 1 cut(s) | 140 |
| BsmFI | GGGAC | 2 cut(s) | 343, 1139 |
| BsmI | GAATGC | 1 cut(s) | 1100 |
| BsnI | GGCC | 4 cut(s) | 12, 182, 885, 977 |
| BsoBI | CYCGRG | 2 cut(s) | 14, 1026 |
| Bsp120I | GGGCCC | 1 cut(s) | 10 |
| Bsp1286I | GDGCHC | 1 cut(s) | 14 |
| Bsp13I | TCCGGA | 1 cut(s) | 1218 |
| Bsp143I | GATC | 4 cut(s) | 271, 812, 940, 1138 |
| BspACI | CCGC | 1 cut(s) | 1098 |
| BspANI | GGCC | 4 cut(s) | 12, 182, 885, 977 |
| BspCNI | CTCAG | 4 cut(s) | 214, 231, 568, 1076 |
| BspEI | TCCGGA | 1 cut(s) | 1218 |
| BspFNI | CGCG | 1 cut(s) | 630 |
| BspHI | TCATGA | 2 cut(s) | 864, 1119 |
| BspLI | GGNNCC | 6 cut(s) | 12, 13, 359, 525, 942, 1140 |
| BspOI | GCTAGC | 1 cut(s) | 336 |
| BspPI | GGATC | 5 cut(s) | 266, 935, 948, 1133, 1146 |
| BspT107I | GGYRCC | 1 cut(s) | 523 |
| BspTI | CTTAAG | 1 cut(s) | 184 |
| BsrDI | GCAATG | 1 cut(s) | 1093 |
| BsrI | ACTGG | 1 cut(s) | 825 |
| BssECI | CCNNGG | 3 cut(s) | 59, 1146, 1170 |
| BssMI | GATC | 4 cut(s) | 271, 812, 940, 1138 |
| BssNI | GRCGYC | 1 cut(s) | 524 |
| BssT1I | CCWWGG | 3 cut(s) | 59, 1146, 1170 |
| Bst2UI | CCWGG | 4 cut(s) | 24, 882, 951, 1181 |
| Bst4CI | ACNGT | 7 cut(s) | 110, 122, 694, 781, 851, 928, 995 |
| Bst6I | CTCTTC | 5 cut(s) | 138, 171, 405, 432, 966 |
| BstACI | GRCGYC | 1 cut(s) | 524 |
| BstAFI | CTTAAG | 1 cut(s) | 184 |
| BstC8I | GCNNGC | 3 cut(s) | 281, 334, 670 |
| BstDEI | CTNAG | 5 cut(s) | 165, 222, 239, 576, 1063 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 575, 948 |
| BstF5I | GGATG | 3 cut(s) | 19, 195, 379 |
| BstFNI | CGCG | 1 cut(s) | 630 |
| BstH2I | RGCGCY | 1 cut(s) | 527 |
| BstHHI | GCGC | 3 cut(s) | 526, 630, 1116 |
| BstKTI | GATC | 4 cut(s) | 274, 815, 943, 1141 |
| BstMAI | GTCTC | 1 cut(s) | 140 |
| BstMBI | GATC | 4 cut(s) | 271, 812, 940, 1138 |
| BstMCI | CGRYCG | 1 cut(s) | 109 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 759 |
| BstNI | CCWGG | 4 cut(s) | 24, 882, 951, 1181 |
| BstNSI | RCATGY | 1 cut(s) | 283 |
| BstSCI | CCNGG | 4 cut(s) | 22, 880, 949, 1179 |
| BstSFI | CTRYAG | 1 cut(s) | 777 |
| BstSLI | GKGCMC | 1 cut(s) | 14 |
| BstUI | CGCG | 1 cut(s) | 630 |
| BstV1I | GCAGC | 3 cut(s) | 323, 762, 839 |
| BstV2I | GAAGAC | 1 cut(s) | 848 |
| BstX2I | RGATCY | 2 cut(s) | 940, 1138 |
| BstYI | RGATCY | 2 cut(s) | 940, 1138 |
| Bsu36I | CCTNAGG | 1 cut(s) | 576 |
| BsuRI | GGCC | 4 cut(s) | 12, 182, 885, 977 |
| BtsCI | GGATG | 3 cut(s) | 19, 195, 379 |
| BtsIMutI | CAGTG | 1 cut(s) | 1000 |
| Cac8I | GCNNGC | 3 cut(s) | 281, 334, 670 |
| CciI | TCATGA | 2 cut(s) | 864, 1119 |
| CfoI | GCGC | 3 cut(s) | 526, 630, 1116 |
| Cfr13I | GGNCC | 3 cut(s) | 10, 11, 357 |
| Csp6I | GTAC | 1 cut(s) | 715 |
| CviAII | CATG | 7 cut(s) | 280, 479, 673, 754, 865, 1120, 1135 |
| CviQI | GTAC | 1 cut(s) | 715 |
| DdeI | CTNAG | 5 cut(s) | 165, 222, 239, 576, 1063 |
| DinI | GGCGCC | 1 cut(s) | 525 |
| DpnI | GATC | 4 cut(s) | 273, 814, 942, 1140 |
| DpnII | GATC | 4 cut(s) | 271, 812, 940, 1138 |
| DraI | TTTAAA | 2 cut(s) | 537, 1002 |
| Eam1104I | CTCTTC | 5 cut(s) | 138, 171, 405, 432, 966 |
| EarI | CTCTTC | 5 cut(s) | 138, 171, 405, 432, 966 |
| Eco130I | CCWWGG | 3 cut(s) | 59, 1146, 1170 |
| Eco147I | AGGCCT | 1 cut(s) | 182 |
| Eco24I | GRGCYC | 1 cut(s) | 14 |
| Eco47I | GGWCC | 1 cut(s) | 357 |
| Eco57I | CTGAAG | 2 cut(s) | 669, 821 |
| Eco81I | CCTNAGG | 1 cut(s) | 576 |
| Eco88I | CYCGRG | 2 cut(s) | 14, 1026 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 575, 948 |
| EcoO109I | RGGNCCY | 2 cut(s) | 11, 357 |
| EcoRII | CCWGG | 4 cut(s) | 22, 880, 949, 1179 |
| EcoT14I | CCWWGG | 3 cut(s) | 59, 1146, 1170 |
| EcoT38I | GRGCYC | 1 cut(s) | 14 |
| EgeI | GGCGCC | 1 cut(s) | 525 |
| EheI | GGCGCC | 1 cut(s) | 525 |
| ErhI | CCWWGG | 3 cut(s) | 59, 1146, 1170 |
| FaeI | CATG | 7 cut(s) | 283, 482, 676, 757, 868, 1123, 1138 |
| FaqI | GGGAC | 2 cut(s) | 343, 1139 |
| FatI | CATG | 7 cut(s) | 279, 478, 672, 753, 864, 1119, 1134 |
| FauI | CCCGC | 1 cut(s) | 1091 |
| Fnu4HI | GCNGC | 3 cut(s) | 337, 751, 828 |
| FokI | GGATG | 3 cut(s) | 6, 202, 386 |
| FriOI | GRGCYC | 1 cut(s) | 14 |
| Fsp4HI | GCNGC | 3 cut(s) | 337, 751, 828 |
| FspBI | CTAG | 2 cut(s) | 198, 333 |
| GlaI | GCGC | 3 cut(s) | 525, 629, 1115 |
| GluI | GCNGC | 3 cut(s) | 337, 751, 828 |
| GsaI | CCCAGC | 1 cut(s) | 1086 |
| GsuI | CTGGAG | 1 cut(s) | 363 |
| HaeII | RGCGCY | 1 cut(s) | 527 |
| HaeIII | GGCC | 4 cut(s) | 12, 182, 885, 977 |
| HapII | CCGG | 1 cut(s) | 1219 |
| HhaI | GCGC | 3 cut(s) | 526, 630, 1116 |
| Hin1I | GRCGYC | 1 cut(s) | 524 |
| Hin1II | CATG | 7 cut(s) | 283, 482, 676, 757, 868, 1123, 1138 |
| Hin6I | GCGC | 3 cut(s) | 524, 628, 1114 |
| HinP1I | GCGC | 3 cut(s) | 524, 628, 1114 |
| HindIII | AAGCTT | 1 cut(s) | 1185 |
| HinfI | GANTC | 1 cut(s) | 241 |
| HpaII | CCGG | 1 cut(s) | 1219 |
| HphI | GGTGA | 5 cut(s) | 106, 359, 515, 635, 863 |
| Hpy166II | GTNNAC | 2 cut(s) | 854, 991 |
| Hpy188I | TCNGA | 2 cut(s) | 325, 840 |
| Hpy188III | TCNNGA | 9 cut(s) | 140, 238, 342, 816, 865, 938, 988, 1120, 1219 |
| Hpy8I | GTNNAC | 2 cut(s) | 854, 991 |
| HpyAV | CCTTC | 6 cut(s) | 109, 146, 409, 436, 646, 1152 |
| HpyCH4III | ACNGT | 7 cut(s) | 110, 122, 694, 781, 851, 928, 995 |
| HpyCH4IV | ACGT | 1 cut(s) | 1036 |
| HpyCH4V | TGCA | 2 cut(s) | 668, 750 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 759 |
| HpyF3I | CTNAG | 5 cut(s) | 165, 222, 239, 576, 1063 |
| HpySE526I | ACGT | 1 cut(s) | 1036 |
| Hsp92I | GRCGYC | 1 cut(s) | 524 |
| Hsp92II | CATG | 7 cut(s) | 283, 482, 676, 757, 868, 1123, 1138 |
| HspAI | GCGC | 3 cut(s) | 524, 628, 1114 |
| KasI | GGCGCC | 1 cut(s) | 523 |
| Kpn2I | TCCGGA | 1 cut(s) | 1218 |
| Kzo9I | GATC | 4 cut(s) | 271, 812, 940, 1138 |
| LmnI | GCTCC | 2 cut(s) | 229, 686 |
| Lsp1109I | GCAGC | 3 cut(s) | 323, 762, 839 |
| LweI | GCATC | 3 cut(s) | 28, 858, 1101 |
| MaeI | CTAG | 2 cut(s) | 198, 333 |
| MaeII | ACGT | 1 cut(s) | 1036 |
| MalI | GATC | 4 cut(s) | 273, 814, 942, 1140 |
| MboI | GATC | 4 cut(s) | 271, 812, 940, 1138 |
| MfeI | CAATTG | 2 cut(s) | 209, 600 |
| MflI | RGATCY | 2 cut(s) | 940, 1138 |
| MhlI | GDGCHC | 1 cut(s) | 14 |
| Mly113I | GGCGCC | 1 cut(s) | 524 |
| MlyI | GAGTC | 1 cut(s) | 250 |
| MroI | TCCGGA | 1 cut(s) | 1218 |
| MseI | TTAA | 6 cut(s) | 125, 185, 257, 285, 536, 1001 |
| MslI | CAYNNNNRTG | 1 cut(s) | 1133 |
| MspCI | CTTAAG | 1 cut(s) | 184 |
| MspI | CCGG | 1 cut(s) | 1219 |
| MspR9I | CCNGG | 4 cut(s) | 24, 882, 951, 1181 |
| MunI | CAATTG | 2 cut(s) | 209, 600 |
| Mva1269I | GAATGC | 1 cut(s) | 1100 |
| MvaI | CCWGG | 4 cut(s) | 24, 882, 951, 1181 |
| MvnI | CGCG | 1 cut(s) | 630 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 759 |
| NarI | GGCGCC | 1 cut(s) | 524 |
| NdeII | GATC | 4 cut(s) | 271, 812, 940, 1138 |
| NheI | GCTAGC | 1 cut(s) | 332 |
| NlaIII | CATG | 7 cut(s) | 283, 482, 676, 757, 868, 1123, 1138 |
| NlaIV | GGNNCC | 6 cut(s) | 12, 13, 359, 525, 942, 1140 |
| NspI | RCATGY | 1 cut(s) | 283 |
| PaeI | GCATGC | 1 cut(s) | 283 |
| PagI | TCATGA | 2 cut(s) | 864, 1119 |
| PceI | AGGCCT | 1 cut(s) | 182 |
| PctI | GAATGC | 1 cut(s) | 1100 |
| PkrI | GCNGC | 3 cut(s) | 338, 752, 829 |
| PleI | GAGTC | 1 cut(s) | 249 |
| PluTI | GGCGCC | 1 cut(s) | 527 |
| PpsI | GAGTC | 1 cut(s) | 249 |
| PpuMI | RGGWCCY | 1 cut(s) | 357 |
| PshBI | ATTAAT | 1 cut(s) | 257 |
| Psp5II | RGGWCCY | 1 cut(s) | 357 |
| Psp6I | CCWGG | 4 cut(s) | 22, 880, 949, 1179 |
| PspFI | CCCAGC | 1 cut(s) | 1082 |
| PspGI | CCWGG | 4 cut(s) | 22, 880, 949, 1179 |
| PspN4I | GGNNCC | 6 cut(s) | 12, 13, 359, 525, 942, 1140 |
| PspOMI | GGGCCC | 1 cut(s) | 10 |
| PspPI | GGNCC | 3 cut(s) | 10, 11, 357 |
| PspPPI | RGGWCCY | 1 cut(s) | 357 |
| PsuI | RGATCY | 2 cut(s) | 940, 1138 |
| RsaI | GTAC | 1 cut(s) | 716 |
| RsaNI | GTAC | 1 cut(s) | 715 |
| RseI | CAYNNNNRTG | 1 cut(s) | 1133 |
| SaqAI | TTAA | 6 cut(s) | 125, 185, 257, 285, 536, 1001 |
| SatI | GCNGC | 3 cut(s) | 337, 751, 828 |
| Sau3AI | GATC | 4 cut(s) | 271, 812, 940, 1138 |
| Sau96I | GGNCC | 3 cut(s) | 10, 11, 357 |
| SchI | GAGTC | 1 cut(s) | 250 |
| ScrFI | CCNGG | 4 cut(s) | 24, 882, 951, 1181 |
| SduI | GDGCHC | 1 cut(s) | 14 |
| SfaNI | GCATC | 3 cut(s) | 28, 858, 1101 |
| SfcI | CTRYAG | 1 cut(s) | 777 |
| SfoI | GGCGCC | 1 cut(s) | 525 |
| SinI | GGWCC | 1 cut(s) | 357 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 1133 |
| SmlI | CTYRAG | 1 cut(s) | 184 |
| SmoI | CTYRAG | 1 cut(s) | 184 |
| SphI | GCATGC | 1 cut(s) | 283 |
| SseBI | AGGCCT | 1 cut(s) | 182 |
| SsiI | CCGC | 1 cut(s) | 1098 |
| SspDI | GGCGCC | 1 cut(s) | 523 |
| SspI | AATATT | 1 cut(s) | 68 |
| SspMI | CTAG | 2 cut(s) | 198, 333 |
| StuI | AGGCCT | 1 cut(s) | 182 |
| StyD4I | CCNGG | 4 cut(s) | 22, 880, 949, 1179 |
| StyI | CCWWGG | 3 cut(s) | 59, 1146, 1170 |
| TaaI | ACNGT | 7 cut(s) | 110, 122, 694, 781, 851, 928, 995 |
| TaiI | ACGT | 1 cut(s) | 1039 |
| TaqI | TCGA | 4 cut(s) | 768, 832, 966, 1092 |
| Tru1I | TTAA | 6 cut(s) | 125, 185, 257, 285, 536, 1001 |
| Tru9I | TTAA | 6 cut(s) | 125, 185, 257, 285, 536, 1001 |
| TscAI | CASTG | 1 cut(s) | 1000 |
| TseI | GCWGC | 3 cut(s) | 336, 750, 827 |
| TspDTI | ATGAA | 8 cut(s) | 423, 450, 483, 853, 984, 1108, 1179, 1227 |
| TspRI | CASTG | 1 cut(s) | 1000 |
| Vha464I | CTTAAG | 1 cut(s) | 184 |
| VpaK11BI | GGWCC | 1 cut(s) | 357 |
| VspI | ATTAAT | 1 cut(s) | 257 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 575, 948 |
| XceI | RCATGY | 1 cut(s) | 283 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 888 |
| XspI | CTAG | 2 cut(s) | 198, 333 |