Domain of unknown function (DUF4283)
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 579 |
| AccI | GTMKAC | 1 cut(s) | 525 |
| AccII | CGCG | 4 cut(s) | 268, 285, 442, 483 |
| AciI | CCGC | 9 cut(s) | 124, 205, 257, 285, 442, 454, 467, 473, 483 |
| AcoI | YGGCCR | 1 cut(s) | 639 |
| AfaI | GTAC | 2 cut(s) | 659, 726 |
| AfeI | AGCGCT | 1 cut(s) | 681 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 508, 509, 591, 667 |
| AjnI | CCWGG | 2 cut(s) | 224, 587 |
| Alw26I | GTCTC | 2 cut(s) | 157, 620 |
| Aor51HI | AGCGCT | 1 cut(s) | 681 |
| AoxI | GGCC | 2 cut(s) | 328, 639 |
| ApeKI | GCWGC | 2 cut(s) | 190, 469 |
| AspLEI | GCGC | 4 cut(s) | 274, 442, 483, 682 |
| AspS9I | GGNCC | 2 cut(s) | 228, 316 |
| AsuC2I | CCSGG | 1 cut(s) | 503 |
| AsuII | TTCGAA | 1 cut(s) | 448 |
| AvaII | GGWCC | 2 cut(s) | 228, 316 |
| BaeGI | GKGCMC | 1 cut(s) | 582 |
| BalI | TGGCCA | 1 cut(s) | 641 |
| BanI | GGYRCC | 1 cut(s) | 579 |
| BanII | GRGCYC | 1 cut(s) | 225 |
| BbvI | GCAGC | 2 cut(s) | 177, 481 |
| BccI | CCATC | 1 cut(s) | 513 |
| BciT130I | CCWGG | 2 cut(s) | 226, 589 |
| BcnI | CCSGG | 1 cut(s) | 503 |
| BcoDI | GTCTC | 2 cut(s) | 157, 620 |
| BfoI | RGCGCY | 1 cut(s) | 683 |
| BisI | GCNGC | 4 cut(s) | 191, 467, 470, 473 |
| BlsI | GCNGC | 4 cut(s) | 192, 468, 471, 474 |
| Bme1390I | CCNGG | 3 cut(s) | 226, 503, 589 |
| Bme18I | GGWCC | 2 cut(s) | 228, 316 |
| BmgT120I | GGNCC | 2 cut(s) | 228, 316 |
| BmiI | GGNNCC | 4 cut(s) | 41, 222, 317, 581 |
| BmrFI | CCNGG | 3 cut(s) | 226, 503, 589 |
| BmsI | GCATC | 3 cut(s) | 82, 82, 422 |
| Bpu10I | CCTNAGC | 1 cut(s) | 132 |
| Bpu14I | TTCGAA | 1 cut(s) | 448 |
| BpuEI | CTTGAG | 1 cut(s) | 26 |
| BpuMI | CCSGG | 1 cut(s) | 503 |
| BsaJI | CCNNGG | 3 cut(s) | 224, 243, 642 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 212, 242, 641, 671 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 508, 509, 591, 667 |
| Bse1I | ACTGG | 1 cut(s) | 753 |
| BseBI | CCWGG | 2 cut(s) | 226, 589 |
| BseDI | CCNNGG | 3 cut(s) | 224, 243, 642 |
| BseGI | GGATG | 1 cut(s) | 437 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 508, 509, 591, 667 |
| BseMII | CTCAG | 1 cut(s) | 191 |
| BseNI | ACTGG | 1 cut(s) | 753 |
| BseSI | GKGCMC | 1 cut(s) | 582 |
| BseXI | GCAGC | 2 cut(s) | 177, 481 |
| BseYI | CCCAGC | 1 cut(s) | 274 |
| Bsh1236I | CGCG | 4 cut(s) | 268, 285, 442, 483 |
| BshFI | GGCC | 2 cut(s) | 330, 641 |
| BshNI | GGYRCC | 1 cut(s) | 579 |
| BsiSI | CCGG | 1 cut(s) | 503 |
| BslFI | GGGAC | 3 cut(s) | 140, 275, 683 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 508, 509, 591, 667 |
| BsmAI | GTCTC | 2 cut(s) | 157, 620 |
| BsmBI | CGTCTC | 1 cut(s) | 157 |
| BsmFI | GGGAC | 3 cut(s) | 140, 275, 683 |
| BsnI | GGCC | 2 cut(s) | 330, 641 |
| Bsp119I | TTCGAA | 1 cut(s) | 448 |
| Bsp1286I | GDGCHC | 2 cut(s) | 225, 582 |
| Bsp143I | GATC | 1 cut(s) | 196 |
| Bsp19I | CCATGG | 1 cut(s) | 642 |
| BspACI | CCGC | 9 cut(s) | 124, 205, 257, 285, 442, 454, 467, 473, 483 |
| BspANI | GGCC | 2 cut(s) | 330, 641 |
| BspCNI | CTCAG | 1 cut(s) | 192 |
| BspFNI | CGCG | 4 cut(s) | 268, 285, 442, 483 |
| BspLI | GGNNCC | 4 cut(s) | 41, 222, 317, 581 |
| BspT104I | TTCGAA | 1 cut(s) | 448 |
| BspT107I | GGYRCC | 1 cut(s) | 579 |
| BsrI | ACTGG | 1 cut(s) | 753 |
| BssECI | CCNNGG | 3 cut(s) | 224, 243, 642 |
| BssMI | GATC | 1 cut(s) | 196 |
| BssT1I | CCWWGG | 2 cut(s) | 243, 642 |
| Bst2UI | CCWGG | 2 cut(s) | 226, 589 |
| Bst4CI | ACNGT | 4 cut(s) | 50, 339, 352, 637 |
| BstBI | TTCGAA | 1 cut(s) | 448 |
| BstC8I | GCNNGC | 2 cut(s) | 178, 270 |
| BstDEI | CTNAG | 4 cut(s) | 34, 132, 200, 608 |
| BstDSI | CCRYGG | 1 cut(s) | 642 |
| BstF5I | GGATG | 1 cut(s) | 437 |
| BstFNI | CGCG | 4 cut(s) | 268, 285, 442, 483 |
| BstH2I | RGCGCY | 1 cut(s) | 683 |
| BstHHI | GCGC | 4 cut(s) | 274, 442, 483, 682 |
| BstKTI | GATC | 1 cut(s) | 199 |
| BstMAI | GTCTC | 2 cut(s) | 157, 620 |
| BstMBI | GATC | 1 cut(s) | 196 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 472, 478 |
| BstNI | CCWGG | 2 cut(s) | 226, 589 |
| BstSCI | CCNGG | 3 cut(s) | 224, 501, 587 |
| BstSLI | GKGCMC | 1 cut(s) | 582 |
| BstUI | CGCG | 4 cut(s) | 268, 285, 442, 483 |
| BstV1I | GCAGC | 2 cut(s) | 177, 481 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 326 |
| BsuRI | GGCC | 2 cut(s) | 330, 641 |
| BtgI | CCRYGG | 1 cut(s) | 642 |
| BtgZI | GCGATG | 1 cut(s) | 532 |
| BtsCI | GGATG | 1 cut(s) | 437 |
| BtsI | GCAGTG | 1 cut(s) | 366 |
| BtsIMutI | CAGTG | 3 cut(s) | 87, 366, 642 |
| Cac8I | GCNNGC | 2 cut(s) | 178, 270 |
| CfoI | GCGC | 4 cut(s) | 274, 442, 483, 682 |
| Cfr13I | GGNCC | 2 cut(s) | 228, 316 |
| CseI | GACGC | 2 cut(s) | 137, 174 |
| Csp6I | GTAC | 2 cut(s) | 658, 725 |
| CviAII | CATG | 3 cut(s) | 326, 346, 643 |
| CviJI | RGCY | 7 cut(s) | 190, 223, 330, 410, 472, 641, 744 |
| CviKI_1 | RGCY | 7 cut(s) | 190, 223, 330, 410, 472, 641, 744 |
| CviQI | GTAC | 2 cut(s) | 658, 725 |
| DdeI | CTNAG | 4 cut(s) | 34, 132, 200, 608 |
| DpnI | GATC | 1 cut(s) | 198 |
| DpnII | GATC | 1 cut(s) | 196 |
| EaeI | YGGCCR | 1 cut(s) | 639 |
| Eco130I | CCWWGG | 2 cut(s) | 243, 642 |
| Eco24I | GRGCYC | 1 cut(s) | 225 |
| Eco47I | GGWCC | 2 cut(s) | 228, 316 |
| Eco47III | AGCGCT | 1 cut(s) | 681 |
| EcoRII | CCWGG | 2 cut(s) | 224, 587 |
| EcoT14I | CCWWGG | 2 cut(s) | 243, 642 |
| EcoT38I | GRGCYC | 1 cut(s) | 225 |
| ErhI | CCWWGG | 2 cut(s) | 243, 642 |
| Esp3I | CGTCTC | 1 cut(s) | 157 |
| FaeI | CATG | 3 cut(s) | 329, 349, 646 |
| FaiI | YATR | 7 cut(s) | 121, 218, 327, 347, 479, 644, 664 |
| FaqI | GGGAC | 3 cut(s) | 140, 275, 683 |
| FatI | CATG | 3 cut(s) | 325, 345, 642 |
| FauI | CCCGC | 4 cut(s) | 117, 198, 435, 476 |
| FblI | GTMKAC | 1 cut(s) | 525 |
| Fnu4HI | GCNGC | 4 cut(s) | 191, 467, 470, 473 |
| FokI | GGATG | 1 cut(s) | 444 |
| FriOI | GRGCYC | 1 cut(s) | 225 |
| Fsp4HI | GCNGC | 4 cut(s) | 191, 467, 470, 473 |
| GlaI | GCGC | 4 cut(s) | 273, 441, 482, 681 |
| GluI | GCNGC | 4 cut(s) | 191, 467, 470, 473 |
| GsaI | CCCAGC | 1 cut(s) | 278 |
| HaeII | RGCGCY | 1 cut(s) | 683 |
| HaeIII | GGCC | 2 cut(s) | 330, 641 |
| HapII | CCGG | 1 cut(s) | 503 |
| HgaI | GACGC | 2 cut(s) | 137, 174 |
| HhaI | GCGC | 4 cut(s) | 274, 442, 483, 682 |
| Hin1II | CATG | 3 cut(s) | 329, 349, 646 |
| Hin6I | GCGC | 4 cut(s) | 272, 440, 481, 680 |
| HinP1I | GCGC | 4 cut(s) | 272, 440, 481, 680 |
| HincII | GTYRAC | 3 cut(s) | 69, 403, 526 |
| HindII | GTYRAC | 3 cut(s) | 69, 403, 526 |
| HinfI | GANTC | 3 cut(s) | 85, 263, 450 |
| HpaI | GTTAAC | 1 cut(s) | 69 |
| HpaII | CCGG | 1 cut(s) | 503 |
| Hpy166II | GTNNAC | 4 cut(s) | 69, 403, 526, 727 |
| Hpy188I | TCNGA | 3 cut(s) | 25, 201, 431 |
| Hpy188III | TCNNGA | 3 cut(s) | 89, 149, 668 |
| Hpy8I | GTNNAC | 4 cut(s) | 69, 403, 526, 727 |
| HpyCH4III | ACNGT | 4 cut(s) | 50, 339, 352, 637 |
| HpyCH4V | TGCA | 1 cut(s) | 325 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 472, 478 |
| HpyF3I | CTNAG | 4 cut(s) | 34, 132, 200, 608 |
| Hsp92II | CATG | 3 cut(s) | 329, 349, 646 |
| HspAI | GCGC | 4 cut(s) | 272, 440, 481, 680 |
| KspAI | GTTAAC | 1 cut(s) | 69 |
| Kzo9I | GATC | 1 cut(s) | 196 |
| LmnI | GCTCC | 2 cut(s) | 220, 514 |
| Lsp1109I | GCAGC | 2 cut(s) | 177, 481 |
| LweI | GCATC | 3 cut(s) | 82, 82, 422 |
| MaeIII | GTNAC | 1 cut(s) | 361 |
| MalI | GATC | 1 cut(s) | 198 |
| MboI | GATC | 1 cut(s) | 196 |
| MboII | GAAGA | 2 cut(s) | 26, 698 |
| MfeI | CAATTG | 1 cut(s) | 320 |
| MhlI | GDGCHC | 2 cut(s) | 225, 582 |
| MlsI | TGGCCA | 1 cut(s) | 641 |
| MluCI | AATT | 5 cut(s) | 238, 320, 369, 561, 731 |
| MluNI | TGGCCA | 1 cut(s) | 641 |
| Mox20I | TGGCCA | 1 cut(s) | 641 |
| MscI | TGGCCA | 1 cut(s) | 641 |
| MseI | TTAA | 2 cut(s) | 68, 341 |
| Msp20I | TGGCCA | 1 cut(s) | 641 |
| MspI | CCGG | 1 cut(s) | 503 |
| MspR9I | CCNGG | 3 cut(s) | 226, 503, 589 |
| MunI | CAATTG | 1 cut(s) | 320 |
| MvaI | CCWGG | 2 cut(s) | 226, 589 |
| MvnI | CGCG | 4 cut(s) | 268, 285, 442, 483 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 472, 478 |
| NciI | CCSGG | 1 cut(s) | 503 |
| NcoI | CCATGG | 1 cut(s) | 642 |
| NdeII | GATC | 1 cut(s) | 196 |
| NlaIII | CATG | 3 cut(s) | 329, 349, 646 |
| NlaIV | GGNNCC | 4 cut(s) | 41, 222, 317, 581 |
| NmuCI | GTSAC | 1 cut(s) | 361 |
| NspV | TTCGAA | 1 cut(s) | 448 |
| PfeI | GAWTC | 3 cut(s) | 85, 263, 450 |
| PkrI | GCNGC | 4 cut(s) | 192, 468, 471, 474 |
| Psp6I | CCWGG | 2 cut(s) | 224, 587 |
| PspFI | CCCAGC | 1 cut(s) | 274 |
| PspGI | CCWGG | 2 cut(s) | 224, 587 |
| PspN4I | GGNNCC | 4 cut(s) | 41, 222, 317, 581 |
| PspPI | GGNCC | 2 cut(s) | 228, 316 |
| RsaI | GTAC | 2 cut(s) | 659, 726 |
| RsaNI | GTAC | 2 cut(s) | 658, 725 |
| SalI | GTCGAC | 1 cut(s) | 524 |
| SaqAI | TTAA | 2 cut(s) | 68, 341 |
| SatI | GCNGC | 4 cut(s) | 191, 467, 470, 473 |
| Sau3AI | GATC | 1 cut(s) | 196 |
| Sau96I | GGNCC | 2 cut(s) | 228, 316 |
| ScrFI | CCNGG | 3 cut(s) | 226, 503, 589 |
| SduI | GDGCHC | 2 cut(s) | 225, 582 |
| SetI | ASST | 5 cut(s) | 66, 402, 596, 654, 719 |
| SfaNI | GCATC | 3 cut(s) | 82, 82, 422 |
| SfuI | TTCGAA | 1 cut(s) | 448 |
| SinI | GGWCC | 2 cut(s) | 228, 316 |
| SmlI | CTYRAG | 1 cut(s) | 5 |
| SmoI | CTYRAG | 1 cut(s) | 5 |
| Sse9I | AATT | 5 cut(s) | 238, 320, 369, 561, 731 |
| SsiI | CCGC | 9 cut(s) | 124, 205, 257, 285, 442, 454, 467, 473, 483 |
| SspI | AATATT | 1 cut(s) | 554 |
| StyD4I | CCNGG | 3 cut(s) | 224, 501, 587 |
| StyI | CCWWGG | 2 cut(s) | 243, 642 |
| TaaI | ACNGT | 4 cut(s) | 50, 339, 352, 637 |
| TaqI | TCGA | 2 cut(s) | 448, 525 |
| TaqII | GACCGA | 1 cut(s) | 66 |
| TasI | AATT | 5 cut(s) | 238, 320, 369, 561, 731 |
| TauI | GCSGC | 2 cut(s) | 469, 475 |
| TfiI | GAWTC | 3 cut(s) | 85, 263, 450 |
| Tru1I | TTAA | 2 cut(s) | 68, 341 |
| Tru9I | TTAA | 2 cut(s) | 68, 341 |
| TscAI | CASTG | 3 cut(s) | 87, 366, 642 |
| TseFI | GTSAC | 1 cut(s) | 361 |
| TseI | GCWGC | 2 cut(s) | 190, 469 |
| Tsp45I | GTSAC | 1 cut(s) | 361 |
| TspRI | CASTG | 3 cut(s) | 87, 366, 642 |
| VpaK11BI | GGWCC | 2 cut(s) | 228, 316 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 649 |
| XmiI | GTMKAC | 1 cut(s) | 525 |