Rh4BG226900

DNA topoisomerase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4B
OG0000022
Physical Location & Seq
Forward (+)
39062412 .. 39074259
11848 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4BG226900.1

Sequence Viewer

Length: 2073 bp
ATGTCTTCAGATCCAGATCATTTCTCATTGGAAGCTTTACTGAATCCCGTTGAAAGACAGGGGATCAAAGCGAGATCCTCCTTTTTGAATGAGATTGGTAAGGAGTTCCAACACTGTTTGGCGGTGAAATTGATTCCTGGAAGGGGTTTTCGTCGCGATGAGTTCATCTCTACATTTATGAACCTCTGGAGATACCATCCTCCAAATAAGGAGGATTTTCTGTTCCAGGAGGATCTGAAGGCAAATCACCGTCAGAAAATGGCTCGTCGTTTTCCAAAAAACATACCAGTAAGTGATTCAGTTATCCAGCTGCCTTCTCCATCAAATTCTGGTATTGGAAACAACCCAACACCGGAAACAGTACCGGCGAAGGCAACGGCGGCGACCACGGGGACTAGAGTTGCAGCAAACCGTTTTTCATATTTGTTGAAGGATAGTTGTGCAGGCCCGGTCTATTTACAAAAGATGAAAGGCCCAAGCCCTAACTATGTTGATTTGCACTTTGACAGGCCCATGCCTATGTTTAGTTCCGATTTGGGTGGGGACAACTCTTACCATACTCATCCGGAGGCGGTTGCTGAATCTAATCCAAGTCCTGCTGATTTTTCTGGGTTGGTTACTGTCCCCTTACATTGCCCAACTGACATTCCGCGTGTGAGTCCAAGAAAATCTCCTCCTACTACATTTTCCCAACCAACGGCTAAGCGAAAATTTGGAGATAGTGGCAAAGCAATATCGGCAGTCCCGAGTGCAGAGGAGGATCTGGTTCATTTTGTTCGTACCAGAAGGCGGGGGAAGCAGTTGAGTAGTTCTTCAAAGGAGAAATCTGACTCTCAGTTGGTGTTATCCAGAATGAGAAGAAAAAGAAGGTTCCATTTTGAGGAAGTGTGGTTGTCTGATCCCAGGTGTTCTCAAGTTATCAAAGATAACTGGGCTGCTTCTATTGCGTTGGGAAACTCTGCACAGGATTGCATTTCTTCTTGTGCTGATGCTCTTATTCATTGGAGTAGAGTTGATTTTGGTAATATTACACACCAGTTGCGAGAGGTGAAACGTAGGTTGGACGGGGCCTATTCAGAGTCTTTCAGAGATGCTAATTTGGCTTCTATGGTGGAATTGGAGACACGGTTTGTGGAGTTGCAGGACAAAGAGGAGGACTTGGGTGGTTGTGATTTCACTCCGATATATGATTGGTATGGAAATGAATTGCAGAAGAAGAAACAGAGGAGTGACAAAGAGAAGAAAGCCGTCAAGCAAGAGGACTTGAAACAACAGCTGAAGTATAAGTGGGCTCTCGTTGATGGTATCAGAAAAGAAGTTATCTTTACCTTTGATATTTTTCACTTCTACTTAGCCAAACTCGAGTTAGACCACCTGGGTTATTTCGAGGACGTGGGGATCGAGCACCCAAAGTCAGGAAAGCTTGTAAGGCGGATTTCTCCAAAAGATGTTACAATAAATACAGGAAAGGATGCCCTGATTCCAGAATGTCCAATCCCTGGTGAAAGCTGGAAAGAAATAAGGCATGACAATACTGTGACATGCAAGGAAGTTGAAGGACTAGATAGGGGAGCTATCAGGGCTGCATACACATGGGATATTACAAGTGAGGATGGTCAAAAGCGGCAGCTAGCAGTGGCTACCTATCTCATGGATAAACATGGTCTGAGGGCTGGAACTGAGAAGGTTGATGAGGATGATGATGAAACTGATATTGTCAGTTGCCGCAGATTAAAAGTAGAGAATTTAAAAGCAATAATATCCCCTATCTTGTTAGAGTTGAACAAGAAAACTATCGAGGGTGATATATCAGCAACCAAGGAGGTTGAAATGGTTTGTAGTCATCAGCGTGATATCGAAGAGCCTCATAGTTCACAAATGTCTAGGATAATGGAGAAGATTGGTGAGCTTCAGGGGACTATAGATGAGATGTTTCAGAGAAACTTGATGTTTATGCCAGTGCAAAAAAGGAGGTTGCTATATATCACTTGTAATTATTTGCGCAGTATCTCAAAGACTGATAGTGCACAAAGCCCTAGGGTAATGCTGAAGATTTACGAGCTCCTGAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Topo_C_assoc Topoisom_I Topoisom_I_N

Protein Analysis

690

Amino Acids

78.47

Weight (kDa)

8.11

Isoelectric Point (pI)

55.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Topoisom_I_N PF02919 379 - 514 6.1e-26 Eukaryotic DNA topoisomerase I, DNA binding fragment
Topoisom_I PF01028 525 - 584 3e-10 Eukaryotic DNA topoisomerase I, catalytic core
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000022)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18231 FvH4_1g19111 FvH4_1g25603 FvH4_1g25612 FvH4_1g27352 FvH4_2g10730 FvH4_2g13120 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g22921 FvH4_3g23592 FvH4_3g23822 FvH4_5g04250 FvH4_5g23151 FvH4_5g37180 FvH4_6g29732 FvH4_7g18821 FvH4_7g32131
prunus_persica Prupe.1G197400_v2.0.a1 Prupe.2G026100_v2.0.a1 Prupe.2G059100_v2.0.a1 Prupe.2G099000_v2.0.a1 Prupe.3G105700_v2.0.a1 Prupe.3G153700_v2.0.a1 Prupe.3G196300_v2.0.a1 Prupe.3G237600_v2.0.a1 Prupe.6G195300_v2.0.a1 Prupe.7G028300_v2.0.a1 Prupe.7G030300_v2.0.a1 Prupe.7G035300_v2.0.a1 Prupe.7G053200_v2.0.a1 Prupe.7G053800_v2.0.a1
pyrus_communis pycom04g19620 pycom10g05030
rosa_chinensis RchiOBHm_Chr3g0468751 RchiOBHm_Chr4g0412211 RchiOBHm_Chr4g0435351 RchiOBHm_Chr4g0435361
rosa_laevigata RLG00000000658 RLG00000004042 RLG00000004625 RLG00000005981 RLG00000007988 RLG00000008105 RLG00000009047 RLG00000011402 RLG00000011415 RLG00000012178 RLG00000012218 RLG00000012251 RLG00000012412 RLG00000012647 RLG00000013541 RLG00000013547 RLG00000013548 RLG00000014198 RLG00000015054 RLG00000015221 RLG00000018021 RLG00000018022 RLG00000018285 RLG00000018569 RLG00000018897 RLG00000019206 RLG00000021674 RLG00000022785 RLG00000023709 RLG00000030006 RLG00000030958 RLG00000032025 RLG00000032026 RLG00000032326 RLG00000032591 RLG00000032797 RLG00000033436 RLG00000033536 RLG00000033572 RLG00000033974 RLG00000034193 RLG00000034718 RLG00000034721 RLG00000035117 RLG00000035348 RLG00000035349 RLG00000035579 RLG00000035708 RLG00000035775 RLG00000036281 RLG00000036638 RLG00000036661 RLG00000036836
rosa_multiflora Rmu_co8092304.1_g000001 Rmu_co8122208.1_g000001 Rmu_co8124144.1_g000001 Rmu_co8176690.1_g000001 Rmu_co8198582.1_g000001 Rmu_co8290821.1_g000001 Rmu_co8299115.1_g000001 Rmu_co8362595.1_g000001 Rmu_co8372359.1_g000001 Rmu_co8402715.1_g000001 Rmu_sc0000077.1_g000014 Rmu_sc0000105.1_g000001 Rmu_sc0000156.1_g000012 Rmu_sc0000338.1_g000021 Rmu_sc0000381.1_g000006 Rmu_sc0000545.1_g000025 Rmu_sc0000556.1_g000013 Rmu_sc0000556.1_g000014 Rmu_sc0000565.1_g000001 Rmu_sc0000580.1_g000005 Rmu_sc0000580.1_g000124 Rmu_sc0000595.1_g000001 Rmu_sc0000608.1_g000006 Rmu_sc0000744.1_g000086 Rmu_sc0000776.1_g000041 Rmu_sc0000791.1_g000060 Rmu_sc0000791.1_g000072 Rmu_sc0000796.1_g000014 Rmu_sc0000876.1_g000019 Rmu_sc0000892.1_g000009 Rmu_sc0000898.1_g000087 Rmu_sc0000948.1_g000026 Rmu_sc0001001.1_g000005 Rmu_sc0001148.1_g000023 Rmu_sc0001148.1_g000027 Rmu_sc0001161.1_g000008 Rmu_sc0001215.1_g000016 Rmu_sc0001251.1_g000019 Rmu_sc0001264.1_g000018 Rmu_sc0001291.1_g000031 Rmu_sc0001293.1_g000015 Rmu_sc0001323.1_g000008 Rmu_sc0001334.1_g000008 Rmu_sc0001356.1_g000001 Rmu_sc0001356.1_g000002 Rmu_sc0001382.1_g000002 Rmu_sc0001436.1_g000012 Rmu_sc0001647.1_g000014 Rmu_sc0001665.1_g000036 Rmu_sc0001698.1_g000013 Rmu_sc0001711.1_g000028 Rmu_sc0001767.1_g000012 Rmu_sc0001836.1_g000042 Rmu_sc0001911.1_g000021 Rmu_sc0001939.1_g000021 Rmu_sc0001940.1_g000012 Rmu_sc0002036.1_g000003 Rmu_sc0002138.1_g000004 Rmu_sc0002154.1_g000008 Rmu_sc0002267.1_g000004 Rmu_sc0002267.1_g000007 Rmu_sc0002451.1_g000016 Rmu_sc0002516.1_g000008 Rmu_sc0002530.1_g000008 Rmu_sc0002571.1_g000012 Rmu_sc0002667.1_g000021 Rmu_sc0002695.1_g000004 Rmu_sc0002784.1_g000031 Rmu_sc0002833.1_g000023 Rmu_sc0002848.1_g000014 Rmu_sc0002945.1_g000003 Rmu_sc0002952.1_g000020 Rmu_sc0002960.1_g000008 Rmu_sc0003060.1_g000001 Rmu_sc0003102.1_g000001 Rmu_sc0003303.1_g000015 Rmu_sc0003354.1_g000011 Rmu_sc0003387.1_g000006 Rmu_sc0003778.1_g000041 Rmu_sc0003859.1_g000045 Rmu_sc0003878.1_g000026 Rmu_sc0004118.1_g000001 Rmu_sc0004212.1_g000008 Rmu_sc0004482.1_g000017 Rmu_sc0004482.1_g000023 Rmu_sc0004618.1_g000036 Rmu_sc0004618.1_g000037 Rmu_sc0004744.1_g000010 Rmu_sc0004762.1_g000009 Rmu_sc0004965.1_g000002 Rmu_sc0004967.1_g000001 Rmu_sc0005173.1_g000014 Rmu_sc0005431.1_g000011 Rmu_sc0005431.1_g000012 Rmu_sc0005431.1_g000022 Rmu_sc0005861.1_g000010 Rmu_sc0005861.1_g000011 Rmu_sc0005913.1_g000019 Rmu_sc0006124.1_g000018 Rmu_sc0006318.1_g000025 Rmu_sc0006616.1_g000012 Rmu_sc0006616.1_g000013 Rmu_sc0006691.1_g000007 Rmu_sc0006776.1_g000007 Rmu_sc0006824.1_g000022 Rmu_sc0006828.1_g000004 Rmu_sc0007059.1_g000005 Rmu_sc0007069.1_g000026 Rmu_sc0007074.1_g000002 Rmu_sc0007081.1_g000002 Rmu_sc0007785.1_g000004 Rmu_sc0007848.1_g000033 Rmu_sc0007883.1_g000007 Rmu_sc0008002.1_g000009 Rmu_sc0008317.1_g000011 Rmu_sc0009180.1_g000007 Rmu_sc0009185.1_g000018 Rmu_sc0010368.1_g000010 Rmu_sc0011733.1_g000008 Rmu_sc0013073.1_g000004 Rmu_sc0013075.1_g000005 Rmu_sc0013280.1_g000003 Rmu_sc0014404.1_g000012 Rmu_sc0014510.1_g000006 Rmu_sc0017005.1_g000006 Rmu_sc0017051.1_g000001 Rmu_sc0017086.1_g000004 Rmu_sc0017394.1_g000004 Rmu_sc0017563.1_g000001 Rmu_sc0018393.1_g000003 Rmu_sc0020047.1_g000001 Rmu_sc0024008.1_g000003 Rmu_sc0026560.1_g000002 Rmu_sc0027933.1_g000001 Rmu_sc0027934.1_g000001 Rmu_sc0029145.1_g000001 Rmu_sc0030686.1_g000002 Rmu_sc0034848.1_g000001 Rmu_sc0036896.1_g000001 Rmu_sc0036897.1_g000001 Rmu_sc0038027.1_g000001 Rmu_sc0041267.1_g000001 Rmu_sc0041948.1_g000001 Rmu_ssc0000011.1_g000021 Rmu_ssc0000019.1_g000009 Rmu_ssc0000127.1_g000005 Rmu_ssc0000135.1_g000011 Rmu_ssc0000264.1_g000029 Rmu_ssc0000432.1_g000047
rosa_roxburghii Rroxscaffold_157G00438300 Rroxscaffold_176G00431540 Rroxscaffold_1G00032330 Rroxscaffold_1G00037330 Rroxscaffold_1G00039780 Rroxscaffold_1G00044330 Rroxscaffold_1G00059840 Rroxscaffold_1G00061850 Rroxscaffold_1G00067020 Rroxscaffold_2G00094770 Rroxscaffold_2G00097970 Rroxscaffold_2G00111380 Rroxscaffold_2G00114430 Rroxscaffold_2G00118370 Rroxscaffold_3G00244430 Rroxscaffold_3G00245450 Rroxscaffold_3G00252790 Rroxscaffold_3G00252800 Rroxscaffold_3G00253930 Rroxscaffold_3G00263900 Rroxscaffold_3G00266650 Rroxscaffold_4G00283310 Rroxscaffold_4G00285100 Rroxscaffold_4G00287050 Rroxscaffold_4G00293050 Rroxscaffold_4G00311960 Rroxscaffold_4G00317580 Rroxscaffold_4G00318840 Rroxscaffold_4G00330920 Rroxscaffold_5G00333040 Rroxscaffold_5G00338150 Rroxscaffold_5G00353130 Rroxscaffold_5G00362630 Rroxscaffold_5G00373970 Rroxscaffold_6G00398030 Rroxscaffold_6G00403960 Rroxscaffold_6G00404110 Rroxscaffold_6G00408470 Rroxscaffold_6G00411860 Rroxscaffold_7G00187970 Rroxscaffold_7G00190970 Rroxscaffold_7G00196980 Rroxscaffold_7G00203070 Rroxscaffold_8G00436830 Rroxscaffold_8G00436840
rosa_rugosa Rorug01G0001300 Rorug01G0273100 Rorug02G0195100 Rorug02G0231300 Rorug02G0441000 Rorug02G0513500 Rorug02G0654500 Rorug03G0189500 Rorug03G0355500 Rorug04G0026700 Rorug04G0103000 Rorug04G0128300 Rorug04G0134700 Rorug04G0203100 Rorug05G0010600 Rorug05G0010700 Rorug05G0010800 Rorug05G0142000 Rorug05G0143200 Rorug05G0158300 Rorug05G0207700 Rorug05G0235800 Rorug06G0014100 Rorug06G0041600 Rorug06G0170300 Rorug07G0106500 Rorug07G0129200 Rorug07G0151100 Rorug07G0210500 Rorug07G0310600
rosa_samantha Rh1AG215600 Rh1AG231300 Rh1BG029100 Rh1BG029200 Rh1BG029300 Rh1BG061600 Rh1BG089300 Rh1BG103900 Rh1BG119800 Rh1BG126600 Rh1BG172800 Rh1BG305900 Rh1CG019500 Rh1CG061200 Rh1CG178900 Rh1CG200100 Rh1CG215200 Rh1DG008800 Rh1DG065300 Rh1DG102700 Rh1DG161600 Rh1DG202700 Rh1DG228800 Rh1DG338100 Rh2AG031400 Rh2AG031500 Rh2AG031600 Rh2AG239800 Rh2AG352400 Rh2AG420700 Rh2AG468100 Rh2BG064800 Rh2BG355600 Rh2BG563200 Rh2CG031200 Rh2CG031300 Rh2CG031400 Rh2CG066300 Rh2CG202000 Rh2CG217900 Rh2CG407600 Rh2CG407700 Rh2CG563800 Rh2DG016500 Rh2DG031100 Rh2DG073700 Rh2DG221200 Rh2DG247700 Rh2DG317700 Rh2DG320500 Rh2DG572500 Rh3BG168200 Rh3CG044300 Rh3CG234500 Rh3CG234600 Rh3CG280700 Rh3CG280800 Rh3CG315800 Rh3DG276800 Rh4AG055600 Rh4AG070200 Rh4AG076400 Rh4AG207200 Rh4AG215300 Rh4BG017000 Rh4BG035100 Rh4BG053700 Rh4BG068000 Rh4BG083600 Rh4BG085100 Rh4BG085400 Rh4BG085500 Rh4BG129100 Rh4BG226900 Rh4CG075900 Rh4CG082300 Rh5AG085800 Rh5AG105400 Rh5AG111000 Rh5AG310300 Rh5AG517300 Rh5AG518000 Rh5BG101600 Rh5BG101700 Rh5BG136100 Rh5BG192800 Rh5BG457600 Rh5BG535800 Rh5CG113500 Rh5CG166800 Rh5CG221300 Rh5CG284500 Rh5CG284600 Rh5CG300000 Rh5CG309400 Rh5CG380000 Rh5DG053700 Rh5DG100200 Rh5DG123700 Rh5DG123800 Rh5DG242500 Rh5DG273200 Rh5DG284200 Rh5DG329000 Rh5DG399400 Rh5DG412700 Rh5DG428300 Rh5DG472200 Rh5DG472300 Rh6AG027400 Rh6AG087000 Rh6AG088900 Rh6AG135900 Rh6AG162100 Rh6AG169800 Rh6AG172500 Rh6AG341600 Rh6AG417800 Rh6BG023500 Rh6BG229100 Rh6BG381600 Rh6BG390400 Rh6CG160200 Rh6CG190500 Rh6CG322500 Rh6DG073200 Rh6DG188800 Rh6DG308300 Rh6DG320800 Rh7AG010500 Rh7AG225800 Rh7AG248100 Rh7AG248200 Rh7AG303700 Rh7AG320800 Rh7AG331700 Rh7AG428300 Rh7AG466200 Rh7BG098200 Rh7BG107900 Rh7BG297200 Rh7BG358100 Rh7CG255500 Rh7CG263600 Rh7CG281500 Rh7CG349100 Rh7DG091300 Rh7DG114500 Rh7DG254200 Rh7DG316800 Rh7DG378500
rosa_wichuraiana Rw0G006200 Rw0G010380 Rw0G018260 Rw1G016250 Rw1G017620 Rw1G018280 Rw1G018390 Rw2G002470 Rw2G008750 Rw2G028260 Rw2G033640 Rw2G033840 Rw4G000510 Rw4G003170 Rw4G004830 Rw4G006220 Rw4G010290 Rw5G009180 Rw5G009680 Rw5G015760 Rw5G017730 Rw5G021440 Rw5G035270 Rw5G042770 Rw6G002880 Rw6G003090 Rw6G003400 Rw6G007620 Rw6G011710 Rw6G017000 Rw6G029770 Rw7G009660 Rw7G020940 Rw7G032200 Rw7G034460 Rw7G034840 Rw7G038460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2003
AccB7I CCANNNNNTGG 1 cut(s) 1499
AccII CGCG 2 cut(s) 156, 652
AccIII TCCGGA 1 cut(s) 565
AciI CCGC 8 cut(s) 122, 380, 572, 650, 790, 1432, 1624, 1726
AclWI GGATC 7 cut(s) 5, 69, 71, 240, 768, 893, 1406
AcsI RAATTY 3 cut(s) 325, 710, 1744
AcuI CTGAAG 4 cut(s) 257, 1298, 1895, 2069
AfaI GTAC 2 cut(s) 363, 781
AfiI CCNNNNNNNGG 7 cut(s) 143, 352, 697, 789, 880, 1415, 1499
AgsI TTSAA 8 cut(s) 53, 88, 430, 816, 1267, 1556, 1783, 1829
AjiI CACGTC 1 cut(s) 1393
AjnI CCWGG 5 cut(s) 136, 225, 902, 1374, 1498
AjuI GAANNNNNNNTTGG 2 cut(s) 1043, 1075
AloI GAACNNNNNNTCC 2 cut(s) 206, 238
Alw21I GWGCWC 3 cut(s) 1407, 2029, 2064
Alw26I GTCTC 1 cut(s) 1115
Alw44I GTGCAC 1 cut(s) 2025
AlwI GGATC 7 cut(s) 5, 69, 71, 240, 768, 893, 1406
Ama87I CYCGRG 2 cut(s) 745, 1361
Aor13HI TCCGGA 1 cut(s) 565
AoxI GGCC 4 cut(s) 445, 472, 509, 1068
ApaLI GTGCAC 1 cut(s) 2025
ApeKI GCWGC 5 cut(s) 310, 404, 935, 1583, 1627
ApoI RAATTY 3 cut(s) 325, 710, 1744
AspA2I CCTAGG 1 cut(s) 2036
AspLEI GCGC 1 cut(s) 2004
AspS9I GGNCC 4 cut(s) 446, 473, 510, 1068
AsuC2I CCSGG 1 cut(s) 449
AsuHPI GGTGA 6 cut(s) 136, 239, 1060, 1514, 1814, 1916
AsuNHI GCTAGC 1 cut(s) 1630
AvaI CYCGRG 2 cut(s) 745, 1361
AvrII CCTAGG 1 cut(s) 2036
BaeGI GKGCMC 1 cut(s) 2029
BanII GRGCYC 2 cut(s) 1294, 2064
Bbv12I GWGCWC 3 cut(s) 1407, 2029, 2064
BbvI GCAGC 5 cut(s) 297, 416, 922, 1570, 1639
BccI CCATC 4 cut(s) 204, 328, 1295, 1607
BceAI ACGGC 3 cut(s) 393, 714, 1232
BciT130I CCWGG 5 cut(s) 138, 227, 904, 1376, 1500
BcnI CCSGG 1 cut(s) 449
BcoDI GTCTC 1 cut(s) 1115
BfaI CTAG 5 cut(s) 396, 1562, 1631, 1884, 2037
BfmI CTRYAG 1 cut(s) 1920
BisI GCNGC 8 cut(s) 311, 381, 405, 936, 1584, 1625, 1628, 1726
BlnI CCTAGG 1 cut(s) 2036
BlpI GCTNAGC 1 cut(s) 702
BlsI GCNGC 8 cut(s) 312, 382, 406, 937, 1585, 1626, 1629, 1727
Bme1390I CCNGG 6 cut(s) 138, 227, 449, 904, 1376, 1500
BmeT110I CYCGRG 2 cut(s) 745, 1361
BmgBI CACGTC 1 cut(s) 1393
BmgT120I GGNCC 4 cut(s) 446, 473, 510, 1068
BmiI GGNNCC 2 cut(s) 872, 1069
BmrFI CCNGG 6 cut(s) 138, 227, 449, 904, 1376, 1500
BmrI ACTGGG 1 cut(s) 940
BmsI GCATC 3 cut(s) 979, 1081, 1462
BmtI GCTAGC 1 cut(s) 1634
BmuI ACTGGG 1 cut(s) 940
BplI GAGNNNNNCTC 2 cut(s) 152, 184
BpmI CTGGAG 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 2066
Bpu1102I GCTNAGC 1 cut(s) 702
BpuEI CTTGAG 1 cut(s) 897
BpuMI CCSGG 1 cut(s) 449
BsaBI GATNNNNATC 1 cut(s) 15
BsaJI CCNNGG 6 cut(s) 387, 902, 1375, 1498, 1818, 2036
BsaWI WCCGGW 2 cut(s) 352, 565
BsaXI ACNNNNNCTCC 2 cut(s) 1112, 1142
Bsc4I CCNNNNNNNGG 7 cut(s) 143, 352, 697, 789, 880, 1415, 1499
Bse118I RCCGGY 1 cut(s) 364
Bse1I ACTGG 4 cut(s) 287, 935, 1036, 1958
Bse3DI GCAATG 1 cut(s) 631
Bse8I GATNNNNATC 1 cut(s) 15
BseAI TCCGGA 1 cut(s) 565
BseBI CCWGG 5 cut(s) 138, 227, 904, 1376, 1500
BseDI CCNNGG 6 cut(s) 387, 902, 1375, 1498, 1818, 2036
BseGI GGATG 5 cut(s) 196, 562, 1477, 1618, 1702
BseJI GATNNNNATC 1 cut(s) 15
BseLI CCNNNNNNNGG 7 cut(s) 143, 352, 697, 789, 880, 1415, 1499
BseMI GCAATG 1 cut(s) 631
BseMII CTCAG 4 cut(s) 848, 1658, 1671, 2057
BseNI ACTGG 4 cut(s) 287, 935, 1036, 1958
BseRI GAGGAG 4 cut(s) 663, 770, 1166, 1240
BseSI GKGCMC 1 cut(s) 2029
BseXI GCAGC 5 cut(s) 297, 416, 922, 1570, 1639
BsgI GTGCAG 3 cut(s) 462, 771, 945
Bsh1236I CGCG 2 cut(s) 156, 652
BshFI GGCC 4 cut(s) 447, 474, 511, 1070
BsiHKAI GWGCWC 3 cut(s) 1407, 2029, 2064
BsiHKCI CYCGRG 2 cut(s) 745, 1361
BsiSI CCGG 4 cut(s) 353, 365, 449, 566
BslFI GGGAC 5 cut(s) 406, 557, 608, 728, 1930
BslI CCNNNNNNNGG 7 cut(s) 143, 352, 697, 789, 880, 1415, 1499
BsmAI GTCTC 1 cut(s) 1115
BsmFI GGGAC 5 cut(s) 406, 557, 608, 728, 1930
BsnI GGCC 4 cut(s) 447, 474, 511, 1070
BsoBI CYCGRG 2 cut(s) 745, 1361
Bsp1286I GDGCHC 4 cut(s) 1294, 1407, 2029, 2064
Bsp13I TCCGGA 1 cut(s) 565
Bsp143I GATC 8 cut(s) 10, 16, 63, 74, 232, 760, 898, 1398
Bsp1720I GCTNAGC 1 cut(s) 702
Bsp68I TCGCGA 1 cut(s) 156
BspACI CCGC 8 cut(s) 122, 380, 572, 650, 790, 1432, 1624, 1726
BspANI GGCC 4 cut(s) 447, 474, 511, 1070
BspCNI CTCAG 4 cut(s) 847, 1659, 1672, 2058
BspEI TCCGGA 1 cut(s) 565
BspFNI CGCG 2 cut(s) 156, 652
BspLI GGNNCC 2 cut(s) 872, 1069
BspOI GCTAGC 1 cut(s) 1634
BspPI GGATC 7 cut(s) 5, 69, 71, 240, 768, 893, 1406
BspQI GCTCTTC 1 cut(s) 1854
BsrDI GCAATG 1 cut(s) 631
BsrFI RCCGGY 1 cut(s) 364
BsrI ACTGG 4 cut(s) 287, 935, 1036, 1958
BssAI RCCGGY 1 cut(s) 364
BssECI CCNNGG 6 cut(s) 387, 902, 1375, 1498, 1818, 2036
BssMI GATC 8 cut(s) 10, 16, 63, 74, 232, 760, 898, 1398
BssT1I CCWWGG 2 cut(s) 1818, 2036
Bst2UI CCWGG 5 cut(s) 138, 227, 904, 1376, 1500
Bst4CI ACNGT 7 cut(s) 116, 251, 361, 413, 622, 1128, 1537
Bst6I CTCTTC 1 cut(s) 1854
BstC8I GCNNGC 2 cut(s) 445, 1632
BstDEI CTNAG 6 cut(s) 702, 834, 1351, 1667, 1680, 2066
BstDSI CCRYGG 1 cut(s) 387
BstF5I GGATG 5 cut(s) 196, 562, 1477, 1618, 1702
BstFNI CGCG 2 cut(s) 156, 652
BstHHI GCGC 1 cut(s) 2004
BstKTI GATC 8 cut(s) 13, 19, 66, 77, 235, 763, 901, 1401
BstMAI GTCTC 1 cut(s) 1115
BstMBI GATC 8 cut(s) 10, 16, 63, 74, 232, 760, 898, 1398
BstMWI GCNNNNNNNGC 7 cut(s) 380, 737, 796, 944, 1100, 1429, 1580
BstNI CCWGG 5 cut(s) 138, 227, 904, 1376, 1500
BstNSI RCATGY 1 cut(s) 1545
BstSCI CCNGG 6 cut(s) 136, 225, 447, 902, 1374, 1498
BstSFI CTRYAG 1 cut(s) 1920
BstSLI GKGCMC 1 cut(s) 2029
BstUI CGCG 2 cut(s) 156, 652
BstV1I GCAGC 5 cut(s) 297, 416, 922, 1570, 1639
BstX2I RGATCY 4 cut(s) 10, 74, 232, 760
BstYI RGATCY 4 cut(s) 10, 74, 232, 760
BsuRI GGCC 4 cut(s) 447, 474, 511, 1070
BtgI CCRYGG 1 cut(s) 387
BtgZI GCGATG 1 cut(s) 171
BtrI CACGTC 1 cut(s) 1393
BtsCI GGATG 5 cut(s) 196, 562, 1477, 1618, 1702
BtsI GCAGTG 1 cut(s) 1641
BtsIMutI CAGTG 3 cut(s) 112, 1641, 1965
BtuMI TCGCGA 1 cut(s) 156
Cac8I GCNNGC 2 cut(s) 445, 1632
CfoI GCGC 1 cut(s) 2004
Cfr10I RCCGGY 1 cut(s) 364
Cfr13I GGNCC 4 cut(s) 446, 473, 510, 1068
Csp6I GTAC 2 cut(s) 362, 780
CviAII CATG 6 cut(s) 514, 1526, 1542, 1593, 1651, 1661
CviQI GTAC 2 cut(s) 362, 780
DdeI CTNAG 6 cut(s) 702, 834, 1351, 1667, 1680, 2066
DpnI GATC 8 cut(s) 12, 18, 65, 76, 234, 762, 900, 1400
DpnII GATC 8 cut(s) 10, 16, 63, 74, 232, 760, 898, 1398
DraI TTTAAA 1 cut(s) 1749
Eam1104I CTCTTC 1 cut(s) 1854
EarI CTCTTC 1 cut(s) 1854
EciI GGCGGA 1 cut(s) 1447
Ecl136II GAGCTC 1 cut(s) 2062
Eco130I CCWWGG 2 cut(s) 1818, 2036
Eco24I GRGCYC 2 cut(s) 1294, 2064
Eco32I GATATC 1 cut(s) 1855
Eco53kI GAGCTC 1 cut(s) 2062
Eco57I CTGAAG 4 cut(s) 257, 1298, 1895, 2069
Eco88I CYCGRG 2 cut(s) 745, 1361
EcoICRI GAGCTC 1 cut(s) 2062
EcoO109I RGGNCCY 1 cut(s) 1068
EcoRII CCWGG 5 cut(s) 136, 225, 902, 1374, 1498
EcoRV GATATC 1 cut(s) 1855
EcoT14I CCWWGG 2 cut(s) 1818, 2036
EcoT38I GRGCYC 2 cut(s) 1294, 2064
ErhI CCWWGG 2 cut(s) 1818, 2036
FaeI CATG 6 cut(s) 517, 1529, 1545, 1596, 1654, 1664
FaqI GGGAC 5 cut(s) 406, 557, 608, 728, 1930
FatI CATG 6 cut(s) 513, 1525, 1541, 1592, 1650, 1660
FauI CCCGC 1 cut(s) 783
Fnu4HI GCNGC 8 cut(s) 311, 381, 405, 936, 1584, 1625, 1628, 1726
FokI GGATG 5 cut(s) 183, 549, 1484, 1625, 1709
FriOI GRGCYC 2 cut(s) 1294, 2064
Fsp4HI GCNGC 8 cut(s) 311, 381, 405, 936, 1584, 1625, 1628, 1726
FspBI CTAG 5 cut(s) 396, 1562, 1631, 1884, 2037
FspI TGCGCA 1 cut(s) 2003
GlaI GCGC 1 cut(s) 2003
GluI GCNGC 8 cut(s) 311, 381, 405, 936, 1584, 1625, 1628, 1726
GsuI CTGGAG 1 cut(s) 208
HaeIII GGCC 4 cut(s) 447, 474, 511, 1070
HapII CCGG 4 cut(s) 353, 365, 449, 566
HhaI GCGC 1 cut(s) 2004
Hin1II CATG 6 cut(s) 517, 1529, 1545, 1596, 1654, 1664
Hin6I GCGC 1 cut(s) 2002
HinP1I GCGC 1 cut(s) 2002
HindIII AAGCTT 2 cut(s) 33, 1421
HinfI GANTC 8 cut(s) 43, 133, 296, 581, 658, 830, 1079, 1480
HpaII CCGG 4 cut(s) 353, 365, 449, 566
HphI GGTGA 6 cut(s) 136, 239, 1060, 1514, 1814, 1916
Hpy166II GTNNAC 2 cut(s) 1874, 2027
Hpy188III TCNNGA 9 cut(s) 14, 155, 187, 566, 745, 849, 1416, 1484, 2065
Hpy8I GTNNAC 2 cut(s) 1874, 2027
Hpy99I CGWCG 2 cut(s) 156, 270
HpyAV CCTTC 9 cut(s) 135, 232, 324, 364, 424, 780, 861, 1550, 1678
HpyCH4III ACNGT 7 cut(s) 116, 251, 361, 413, 622, 1128, 1537
HpyCH4IV ACGT 2 cut(s) 1054, 1392
HpyF10VI GCNNNNNNNGC 7 cut(s) 380, 737, 796, 944, 1100, 1429, 1580
HpyF3I CTNAG 6 cut(s) 702, 834, 1351, 1667, 1680, 2066
HpySE526I ACGT 2 cut(s) 1054, 1392
Hsp92II CATG 6 cut(s) 517, 1529, 1545, 1596, 1654, 1664
HspAI GCGC 1 cut(s) 2002
Kpn2I TCCGGA 1 cut(s) 565
Kzo9I GATC 8 cut(s) 10, 16, 63, 74, 232, 760, 898, 1398
LguI GCTCTTC 1 cut(s) 1854
LmnI GCTCC 2 cut(s) 1571, 2067
Lsp1109I GCAGC 5 cut(s) 297, 416, 922, 1570, 1639
LweI GCATC 3 cut(s) 979, 1081, 1462
MaeI CTAG 5 cut(s) 396, 1562, 1631, 1884, 2037
MaeII ACGT 2 cut(s) 1054, 1392
MaeIII GTNAC 4 cut(s) 616, 1229, 1450, 1537
MalI GATC 8 cut(s) 12, 18, 65, 76, 234, 762, 900, 1400
MboI GATC 8 cut(s) 10, 16, 63, 74, 232, 760, 898, 1398
MboII GAAGA 9 cut(s) 804, 870, 969, 1225, 1228, 1252, 1871, 1909, 2062
MflI RGATCY 4 cut(s) 10, 74, 232, 760
MhlI GDGCHC 4 cut(s) 1294, 1407, 2029, 2064
MluCI AATT 8 cut(s) 128, 325, 710, 1096, 1115, 1205, 1744, 1993
MlyI GAGTC 3 cut(s) 667, 824, 1088
MmeI TCCRAC 2 cut(s) 133, 1041
MroI TCCGGA 1 cut(s) 565
MseI TTAA 2 cut(s) 1733, 1748
MslI CAYNNNNRTG 3 cut(s) 518, 1591, 1848
MspA1I CMGCKG 2 cut(s) 310, 1276
MspI CCGG 4 cut(s) 353, 365, 449, 566
MspR9I CCNGG 6 cut(s) 138, 227, 449, 904, 1376, 1500
MvaI CCWGG 5 cut(s) 138, 227, 904, 1376, 1500
MvnI CGCG 2 cut(s) 156, 652
MwoI GCNNNNNNNGC 7 cut(s) 380, 737, 796, 944, 1100, 1429, 1580
NciI CCSGG 1 cut(s) 449
NdeII GATC 8 cut(s) 10, 16, 63, 74, 232, 760, 898, 1398
NheI GCTAGC 1 cut(s) 1630
NlaIII CATG 6 cut(s) 517, 1529, 1545, 1596, 1654, 1664
NlaIV GGNNCC 2 cut(s) 872, 1069
NmuCI GTSAC 2 cut(s) 1229, 1537
NruI TCGCGA 1 cut(s) 156
NsbI TGCGCA 1 cut(s) 2003
NspI RCATGY 1 cut(s) 1545
PaeR7I CTCGAG 1 cut(s) 1361
PciSI GCTCTTC 1 cut(s) 1854
PcsI WCGNNNNNNNCGW 2 cut(s) 743, 1398
PfeI GAWTC 5 cut(s) 43, 133, 296, 581, 1480
PflMI CCANNNNNTGG 1 cut(s) 1499
PfoI TCCNGGA 2 cut(s) 136, 225
PkrI GCNGC 8 cut(s) 312, 382, 406, 937, 1585, 1626, 1629, 1727
PleI GAGTC 3 cut(s) 666, 824, 1087
PpsI GAGTC 3 cut(s) 666, 824, 1087
Psp124BI GAGCTC 1 cut(s) 2064
Psp6I CCWGG 5 cut(s) 136, 225, 902, 1374, 1498
PspGI CCWGG 5 cut(s) 136, 225, 902, 1374, 1498
PspN4I GGNNCC 2 cut(s) 872, 1069
PspPI GGNCC 4 cut(s) 446, 473, 510, 1068
PspXI VCTCGAGB 1 cut(s) 1361
PsuI RGATCY 4 cut(s) 10, 74, 232, 760
PvuII CAGCTG 2 cut(s) 310, 1276
RruI TCGCGA 1 cut(s) 156
RsaI GTAC 2 cut(s) 363, 781
RsaNI GTAC 2 cut(s) 362, 780
RseI CAYNNNNRTG 3 cut(s) 518, 1591, 1848
SacI GAGCTC 1 cut(s) 2064
SapI GCTCTTC 1 cut(s) 1854
SaqAI TTAA 2 cut(s) 1733, 1748
SatI GCNGC 8 cut(s) 311, 381, 405, 936, 1584, 1625, 1628, 1726
Sau3AI GATC 8 cut(s) 10, 16, 63, 74, 232, 760, 898, 1398
Sau96I GGNCC 4 cut(s) 446, 473, 510, 1068
SchI GAGTC 3 cut(s) 667, 824, 1088
ScrFI CCNGG 6 cut(s) 138, 227, 449, 904, 1376, 1500
SduI GDGCHC 4 cut(s) 1294, 1407, 2029, 2064
SfaNI GCATC 3 cut(s) 979, 1081, 1462
SfcI CTRYAG 1 cut(s) 1920
Sfr274I CTCGAG 1 cut(s) 1361
SlaI CTCGAG 1 cut(s) 1361
SmiMI CAYNNNNRTG 3 cut(s) 518, 1591, 1848
SmlI CTYRAG 2 cut(s) 912, 1361
SmoI CTYRAG 2 cut(s) 912, 1361
Sse9I AATT 8 cut(s) 128, 325, 710, 1096, 1115, 1205, 1744, 1993
SsiI CCGC 8 cut(s) 122, 380, 572, 650, 790, 1432, 1624, 1726
SspI AATATT 1 cut(s) 1027
SspMI CTAG 5 cut(s) 396, 1562, 1631, 1884, 2037
SstI GAGCTC 1 cut(s) 2064
StyD4I CCNGG 6 cut(s) 136, 225, 447, 902, 1374, 1498
StyI CCWWGG 2 cut(s) 1818, 2036
TaaI ACNGT 7 cut(s) 116, 251, 361, 413, 622, 1128, 1537
TaiI ACGT 2 cut(s) 1057, 1395
TaqI TCGA 5 cut(s) 1362, 1386, 1401, 1797, 1857
TasI AATT 8 cut(s) 128, 325, 710, 1096, 1115, 1205, 1744, 1993
TauI GCSGC 3 cut(s) 383, 1627, 1728
TfiI GAWTC 5 cut(s) 43, 133, 296, 581, 1480
Tru1I TTAA 2 cut(s) 1733, 1748
Tru9I TTAA 2 cut(s) 1733, 1748
TscAI CASTG 3 cut(s) 119, 1641, 1965
TseFI GTSAC 2 cut(s) 1229, 1537
TseI GCWGC 5 cut(s) 310, 404, 935, 1583, 1627
Tsp45I GTSAC 2 cut(s) 1229, 1537
TspDTI ATGAA 8 cut(s) 154, 194, 408, 482, 758, 989, 1218, 1719
TspRI CASTG 3 cut(s) 119, 1641, 1965
Van91I CCANNNNNTGG 1 cut(s) 1499
VneI GTGCAC 1 cut(s) 2025
XapI RAATTY 3 cut(s) 325, 710, 1744
XceI RCATGY 1 cut(s) 1545
XhoI CTCGAG 1 cut(s) 1361
XmaJI CCTAGG 1 cut(s) 2036
XspI CTAG 5 cut(s) 396, 1562, 1631, 1884, 2037
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.