Rorug05G0158300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0000022
Physical Location & Seq
Forward (+)
14729568 .. 14732264
2697 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0158300.1

Sequence Viewer

Length: 2697 bp
ATGTCCTCCTCCTTCTCCGCCCACCTCCTCTCCAAACCTCTACACACATTTTCTTCCCTCCAAATCCAACAACCCACATCTCCCAAAAGGACCACTCCACCCCCCAACTCCTCTCTTCTCAACCACCCCATCACCACCGCCACCACCTCCCCACCACCCAAACCCATCTCCCATTCCCGCTCATGGGTCGACACCATTCGCTCCCGAACCCGGTCCGGTCACTACAAAGAAGCCATCTCAACCTACATCGAAATGACCCGTTCCGGTATCGAACCCGACAACTTTGCCTTCCCGGCGGTCCTCAAGGCCGTCACGGCCCTTCAAGACTTGAACTTGGGGAAGCAGACACACGCCCATGTTGTCAAATTTGGATACGAGTCATCTTCTGTCACCGTGGCGAATACTCTTGTCAATGTGTATGGGAAATGTGGCGATATTGGGGATGCGTATAAGGTGTTTGATGGAATGACTGAGAGGGATCAGGTTTCTTGGAATTCCATGATTGCTGCTTTGTGTAGGTTTGAGGAATGGGAGCTGGCTTTGGAAGCGTTTAGGTCGATGTTTTCAGATAATGTGGAGCCTAGCTCGTTTACTTTGGTGAGTGCTGCACTTGCTTGTTCGAATTTGGATAGGCGTGATGGGTTGAGGCTTGGGAAGCAAGTTCATGCGTATAGTATGAGAATGTGTGAGTCGAAAACTTTTACTGTCAATGCTTTGATGTCTATGTATGCTAAGTTGGGGATGGTGGGATACTCGAGAGGTGTGTTTGAGTTGTTTGAGGAGTGTGACCTGGTTTCGTGGAACACCATGATAAGTTCACTGTCTCAGAATGACCGGTTTGTGGAGGCATTAGAGTTCTTCCGGTTGATGATTCTCGAGGGAATCAGACCAGATGGGGTGACGGTTGCGAGTGTTCTTCCTGCATGTTCTCATTTGGAGATGTTGGAGGCAGGGAAGGAAATTCATGCTTATGCATTGAGAGCCAATGAGTTAACTGGGAATTCTTATGTGGGCAGTGCTTTAGTTGACATGTATTGCAACTGCCGGCAAGTTGAGAGTGGTCGTCGAGTTTTTGATGCTGTCATGGAGCGGAGAGTTCCACTTTGGAATGCAATGATTACTGGTTATGCACAAAACGAGTATGATGAGGAGGCCTTGGACCTGTTCCTTGAAATGTATGCTGTGTCGGGGCATAATCCAAATGCCACCACAATGTCGAGTATTGTGCCAGCATGTGTGCGGTCTGAAGCATTCTCGGGGAAAGAAAGTATCCATGGTTTTGTAATAAAGCGGAGTCTGGAGAAGAACAGGTACGTGCAAAATGCACTTATGGACATGTACTCGAGGATGGGGAGAACAGAAATTTCAGAAACTATATTTAACAGCATGGAAGGAAAAGACATTGTATCATGGAACACCATGATCACTGGTTATGTTATTTCCAGACGTCATGATGACGCACTTAATCTGCTATATGAAATGCAAAGAGTAGAAGAAAACAAGAACACCAATGGTACTGGGTATGATAATGAAAGAAGGGTTCCCCTTAAGCCAAATACTATAACACTCGTGACTCTTCTTCCTAGTTGTGCTGTATTATCAGCACTCGCTAAAGGGAAAGAGATCCATGCTTATGCTACTAGACATTTGTTGGCACTAGATATTGCTGTAGGTAGTGCACTGGTTGACATGTATGCTAAATGTGGCTGCTTAGATTTATCTAGAGCCATGTTTAATCAGATGCCGTTGAAGAATGTGATCACCTGGAATGTGCTTATCATGGCTTATGGTATGCATGGAAGAGGAGAGGAAGCCTTAGAACTCTTTAGGAATATGGTGGATGAAGGAAGATGGAATAAAGAGATAAGGCCTAATGAAGTTACATTCATTGCAATTTTTGCAGCTTGTAGTCATTCTGGGATGGTAGGTGAAGGTCTGAACTTGTTCCACACAATGAAACAAGACCACGGCATTGAACCTGCACCAGACCATTATGCTTGTGTTGTGGATTTGCTTGGTCGGGCTGGTAGTGTGGAGCGAGCATATGAAATCATCAAAACCATGCCTTCTGAGTTTGATAAAGCAGGTGCCTGGAGTAGCTTGCTTGGTGCTTGTCGACTCCACCAGAATGTAGAGATTGGAGAAATTGCAGCTAATCATCTCCTGCAGCTGGAGCCTGATGTGGCTAGTCACTATGTTCTACTCTCTAATATCTACTCATCTTCTGGACTTTGGGAAAAGGCCATGGATCTCCGGAGAAAAATGAAGGAAATGGGAGTGAGAAAAGAGCCTGGTTGTAGTTGGATCGAGTTTGAAGATGAGGTTCATAAGTTTTTGGCTGGGGATCTGTCGCACCCACAAAGTGAGCAGCTCCATGAATATCTCGAAACCCTGTCAGAAAGGATGAAAAAGGAGGGTTATGTGCCTGATACTTCTTGTGTACTTCACAATGTTGATGAGGACGAGAAGGAAACTTTACTCTGTGGGCACAGCGAGAAACTGGCAATGGCTTTCGGCCTCCTCAATACCCGTCCAGGGACTACCATTAGAGTTGCCAAGAACCTTAGAGTCTGTAATGACTGCCACCTTGCAGCGAAATATATCTCAAAGATGCTGGACAGGGAGATCATTTTAAGAGATGTAAGAAGGTTCCATCATTTTAGAAATGGAAATTGTTCATGTGGAGATTATTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

898

Amino Acids

100.82

Weight (kDa)

6.06

Isoelectric Point (pI)

52.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 160 - 198 1.2e-07 PPR repeat family
PPR_2 PF13041 263 - 310 7.5e-08 PPR repeat family
PPR PF01535 265 - 295 4e-07 PPR repeat
PPR_2 PF13041 368 - 413 5.9e-06 PPR repeat family
PPR PF01535 469 - 496 7.6e-06 PPR repeat
PPR_2 PF13041 584 - 616 1.1e-06 PPR repeat family
PPR PF01535 587 - 616 3.4e-08 PPR repeat
E_motif PF20431 708 - 770 3.6e-21 E motif
Eplus_motif PF20430 775 - 802 8.2e-07 E+ motif
DYW_deaminase PF14432 806 - 898 7.3e-45 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000022)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g18231 FvH4_1g19111 FvH4_1g25603 FvH4_1g25612 FvH4_1g27352 FvH4_2g10730 FvH4_2g13120 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g05891 FvH4_3g22921 FvH4_3g23592 FvH4_3g23822 FvH4_5g04250 FvH4_5g23151 FvH4_5g37180 FvH4_6g29732 FvH4_7g18821 FvH4_7g32131
prunus_persica Prupe.1G197400_v2.0.a1 Prupe.2G026100_v2.0.a1 Prupe.2G059100_v2.0.a1 Prupe.2G099000_v2.0.a1 Prupe.3G105700_v2.0.a1 Prupe.3G153700_v2.0.a1 Prupe.3G196300_v2.0.a1 Prupe.3G237600_v2.0.a1 Prupe.6G195300_v2.0.a1 Prupe.7G028300_v2.0.a1 Prupe.7G030300_v2.0.a1 Prupe.7G035300_v2.0.a1 Prupe.7G053200_v2.0.a1 Prupe.7G053800_v2.0.a1
pyrus_communis pycom04g19620 pycom10g05030
rosa_chinensis RchiOBHm_Chr3g0468751 RchiOBHm_Chr4g0412211 RchiOBHm_Chr4g0435351 RchiOBHm_Chr4g0435361
rosa_laevigata RLG00000000658 RLG00000004042 RLG00000004625 RLG00000005981 RLG00000007988 RLG00000008105 RLG00000009047 RLG00000011402 RLG00000011415 RLG00000012178 RLG00000012218 RLG00000012251 RLG00000012412 RLG00000012647 RLG00000013541 RLG00000013547 RLG00000013548 RLG00000014198 RLG00000015054 RLG00000015221 RLG00000018021 RLG00000018022 RLG00000018285 RLG00000018569 RLG00000018897 RLG00000019206 RLG00000021674 RLG00000022785 RLG00000023709 RLG00000030006 RLG00000030958 RLG00000032025 RLG00000032026 RLG00000032326 RLG00000032591 RLG00000032797 RLG00000033436 RLG00000033536 RLG00000033572 RLG00000033974 RLG00000034193 RLG00000034718 RLG00000034721 RLG00000035117 RLG00000035348 RLG00000035349 RLG00000035579 RLG00000035708 RLG00000035775 RLG00000036281 RLG00000036638 RLG00000036661 RLG00000036836
rosa_multiflora Rmu_co8092304.1_g000001 Rmu_co8122208.1_g000001 Rmu_co8124144.1_g000001 Rmu_co8176690.1_g000001 Rmu_co8198582.1_g000001 Rmu_co8290821.1_g000001 Rmu_co8299115.1_g000001 Rmu_co8362595.1_g000001 Rmu_co8372359.1_g000001 Rmu_co8402715.1_g000001 Rmu_sc0000077.1_g000014 Rmu_sc0000105.1_g000001 Rmu_sc0000156.1_g000012 Rmu_sc0000338.1_g000021 Rmu_sc0000381.1_g000006 Rmu_sc0000545.1_g000025 Rmu_sc0000556.1_g000013 Rmu_sc0000556.1_g000014 Rmu_sc0000565.1_g000001 Rmu_sc0000580.1_g000005 Rmu_sc0000580.1_g000124 Rmu_sc0000595.1_g000001 Rmu_sc0000608.1_g000006 Rmu_sc0000744.1_g000086 Rmu_sc0000776.1_g000041 Rmu_sc0000791.1_g000060 Rmu_sc0000791.1_g000072 Rmu_sc0000796.1_g000014 Rmu_sc0000876.1_g000019 Rmu_sc0000892.1_g000009 Rmu_sc0000898.1_g000087 Rmu_sc0000948.1_g000026 Rmu_sc0001001.1_g000005 Rmu_sc0001148.1_g000023 Rmu_sc0001148.1_g000027 Rmu_sc0001161.1_g000008 Rmu_sc0001215.1_g000016 Rmu_sc0001251.1_g000019 Rmu_sc0001264.1_g000018 Rmu_sc0001291.1_g000031 Rmu_sc0001293.1_g000015 Rmu_sc0001323.1_g000008 Rmu_sc0001334.1_g000008 Rmu_sc0001356.1_g000001 Rmu_sc0001356.1_g000002 Rmu_sc0001382.1_g000002 Rmu_sc0001436.1_g000012 Rmu_sc0001647.1_g000014 Rmu_sc0001665.1_g000036 Rmu_sc0001698.1_g000013 Rmu_sc0001711.1_g000028 Rmu_sc0001767.1_g000012 Rmu_sc0001836.1_g000042 Rmu_sc0001911.1_g000021 Rmu_sc0001939.1_g000021 Rmu_sc0001940.1_g000012 Rmu_sc0002036.1_g000003 Rmu_sc0002138.1_g000004 Rmu_sc0002154.1_g000008 Rmu_sc0002267.1_g000004 Rmu_sc0002267.1_g000007 Rmu_sc0002451.1_g000016 Rmu_sc0002516.1_g000008 Rmu_sc0002530.1_g000008 Rmu_sc0002571.1_g000012 Rmu_sc0002667.1_g000021 Rmu_sc0002695.1_g000004 Rmu_sc0002784.1_g000031 Rmu_sc0002833.1_g000023 Rmu_sc0002848.1_g000014 Rmu_sc0002945.1_g000003 Rmu_sc0002952.1_g000020 Rmu_sc0002960.1_g000008 Rmu_sc0003060.1_g000001 Rmu_sc0003102.1_g000001 Rmu_sc0003303.1_g000015 Rmu_sc0003354.1_g000011 Rmu_sc0003387.1_g000006 Rmu_sc0003778.1_g000041 Rmu_sc0003859.1_g000045 Rmu_sc0003878.1_g000026 Rmu_sc0004118.1_g000001 Rmu_sc0004212.1_g000008 Rmu_sc0004482.1_g000017 Rmu_sc0004482.1_g000023 Rmu_sc0004618.1_g000036 Rmu_sc0004618.1_g000037 Rmu_sc0004744.1_g000010 Rmu_sc0004762.1_g000009 Rmu_sc0004965.1_g000002 Rmu_sc0004967.1_g000001 Rmu_sc0005173.1_g000014 Rmu_sc0005431.1_g000011 Rmu_sc0005431.1_g000012 Rmu_sc0005431.1_g000022 Rmu_sc0005861.1_g000010 Rmu_sc0005861.1_g000011 Rmu_sc0005913.1_g000019 Rmu_sc0006124.1_g000018 Rmu_sc0006318.1_g000025 Rmu_sc0006616.1_g000012 Rmu_sc0006616.1_g000013 Rmu_sc0006691.1_g000007 Rmu_sc0006776.1_g000007 Rmu_sc0006824.1_g000022 Rmu_sc0006828.1_g000004 Rmu_sc0007059.1_g000005 Rmu_sc0007069.1_g000026 Rmu_sc0007074.1_g000002 Rmu_sc0007081.1_g000002 Rmu_sc0007785.1_g000004 Rmu_sc0007848.1_g000033 Rmu_sc0007883.1_g000007 Rmu_sc0008002.1_g000009 Rmu_sc0008317.1_g000011 Rmu_sc0009180.1_g000007 Rmu_sc0009185.1_g000018 Rmu_sc0010368.1_g000010 Rmu_sc0011733.1_g000008 Rmu_sc0013073.1_g000004 Rmu_sc0013075.1_g000005 Rmu_sc0013280.1_g000003 Rmu_sc0014404.1_g000012 Rmu_sc0014510.1_g000006 Rmu_sc0017005.1_g000006 Rmu_sc0017051.1_g000001 Rmu_sc0017086.1_g000004 Rmu_sc0017394.1_g000004 Rmu_sc0017563.1_g000001 Rmu_sc0018393.1_g000003 Rmu_sc0020047.1_g000001 Rmu_sc0024008.1_g000003 Rmu_sc0026560.1_g000002 Rmu_sc0027933.1_g000001 Rmu_sc0027934.1_g000001 Rmu_sc0029145.1_g000001 Rmu_sc0030686.1_g000002 Rmu_sc0034848.1_g000001 Rmu_sc0036896.1_g000001 Rmu_sc0036897.1_g000001 Rmu_sc0038027.1_g000001 Rmu_sc0041267.1_g000001 Rmu_sc0041948.1_g000001 Rmu_ssc0000011.1_g000021 Rmu_ssc0000019.1_g000009 Rmu_ssc0000127.1_g000005 Rmu_ssc0000135.1_g000011 Rmu_ssc0000264.1_g000029 Rmu_ssc0000432.1_g000047
rosa_roxburghii Rroxscaffold_157G00438300 Rroxscaffold_176G00431540 Rroxscaffold_1G00032330 Rroxscaffold_1G00037330 Rroxscaffold_1G00039780 Rroxscaffold_1G00044330 Rroxscaffold_1G00059840 Rroxscaffold_1G00061850 Rroxscaffold_1G00067020 Rroxscaffold_2G00094770 Rroxscaffold_2G00097970 Rroxscaffold_2G00111380 Rroxscaffold_2G00114430 Rroxscaffold_2G00118370 Rroxscaffold_3G00244430 Rroxscaffold_3G00245450 Rroxscaffold_3G00252790 Rroxscaffold_3G00252800 Rroxscaffold_3G00253930 Rroxscaffold_3G00263900 Rroxscaffold_3G00266650 Rroxscaffold_4G00283310 Rroxscaffold_4G00285100 Rroxscaffold_4G00287050 Rroxscaffold_4G00293050 Rroxscaffold_4G00311960 Rroxscaffold_4G00317580 Rroxscaffold_4G00318840 Rroxscaffold_4G00330920 Rroxscaffold_5G00333040 Rroxscaffold_5G00338150 Rroxscaffold_5G00353130 Rroxscaffold_5G00362630 Rroxscaffold_5G00373970 Rroxscaffold_6G00398030 Rroxscaffold_6G00403960 Rroxscaffold_6G00404110 Rroxscaffold_6G00408470 Rroxscaffold_6G00411860 Rroxscaffold_7G00187970 Rroxscaffold_7G00190970 Rroxscaffold_7G00196980 Rroxscaffold_7G00203070 Rroxscaffold_8G00436830 Rroxscaffold_8G00436840
rosa_rugosa Rorug01G0001300 Rorug01G0273100 Rorug02G0195100 Rorug02G0231300 Rorug02G0441000 Rorug02G0513500 Rorug02G0654500 Rorug03G0189500 Rorug03G0355500 Rorug04G0026700 Rorug04G0103000 Rorug04G0128300 Rorug04G0134700 Rorug04G0203100 Rorug05G0010600 Rorug05G0010700 Rorug05G0010800 Rorug05G0142000 Rorug05G0143200 Rorug05G0158300 Rorug05G0207700 Rorug05G0235800 Rorug06G0014100 Rorug06G0041600 Rorug06G0170300 Rorug07G0106500 Rorug07G0129200 Rorug07G0151100 Rorug07G0210500 Rorug07G0310600
rosa_samantha Rh1AG215600 Rh1AG231300 Rh1BG029100 Rh1BG029200 Rh1BG029300 Rh1BG061600 Rh1BG089300 Rh1BG103900 Rh1BG119800 Rh1BG126600 Rh1BG172800 Rh1BG305900 Rh1CG019500 Rh1CG061200 Rh1CG178900 Rh1CG200100 Rh1CG215200 Rh1DG008800 Rh1DG065300 Rh1DG102700 Rh1DG161600 Rh1DG202700 Rh1DG228800 Rh1DG338100 Rh2AG031400 Rh2AG031500 Rh2AG031600 Rh2AG239800 Rh2AG352400 Rh2AG420700 Rh2AG468100 Rh2BG064800 Rh2BG355600 Rh2BG563200 Rh2CG031200 Rh2CG031300 Rh2CG031400 Rh2CG066300 Rh2CG202000 Rh2CG217900 Rh2CG407600 Rh2CG407700 Rh2CG563800 Rh2DG016500 Rh2DG031100 Rh2DG073700 Rh2DG221200 Rh2DG247700 Rh2DG317700 Rh2DG320500 Rh2DG572500 Rh3BG168200 Rh3CG044300 Rh3CG234500 Rh3CG234600 Rh3CG280700 Rh3CG280800 Rh3CG315800 Rh3DG276800 Rh4AG055600 Rh4AG070200 Rh4AG076400 Rh4AG207200 Rh4AG215300 Rh4BG017000 Rh4BG035100 Rh4BG053700 Rh4BG068000 Rh4BG083600 Rh4BG085100 Rh4BG085400 Rh4BG085500 Rh4BG129100 Rh4BG226900 Rh4CG075900 Rh4CG082300 Rh5AG085800 Rh5AG105400 Rh5AG111000 Rh5AG310300 Rh5AG517300 Rh5AG518000 Rh5BG101600 Rh5BG101700 Rh5BG136100 Rh5BG192800 Rh5BG457600 Rh5BG535800 Rh5CG113500 Rh5CG166800 Rh5CG221300 Rh5CG284500 Rh5CG284600 Rh5CG300000 Rh5CG309400 Rh5CG380000 Rh5DG053700 Rh5DG100200 Rh5DG123700 Rh5DG123800 Rh5DG242500 Rh5DG273200 Rh5DG284200 Rh5DG329000 Rh5DG399400 Rh5DG412700 Rh5DG428300 Rh5DG472200 Rh5DG472300 Rh6AG027400 Rh6AG087000 Rh6AG088900 Rh6AG135900 Rh6AG162100 Rh6AG169800 Rh6AG172500 Rh6AG341600 Rh6AG417800 Rh6BG023500 Rh6BG229100 Rh6BG381600 Rh6BG390400 Rh6CG160200 Rh6CG190500 Rh6CG322500 Rh6DG073200 Rh6DG188800 Rh6DG308300 Rh6DG320800 Rh7AG010500 Rh7AG225800 Rh7AG248100 Rh7AG248200 Rh7AG303700 Rh7AG320800 Rh7AG331700 Rh7AG428300 Rh7AG466200 Rh7BG098200 Rh7BG107900 Rh7BG297200 Rh7BG358100 Rh7CG255500 Rh7CG263600 Rh7CG281500 Rh7CG349100 Rh7DG091300 Rh7DG114500 Rh7DG254200 Rh7DG316800 Rh7DG378500
rosa_wichuraiana Rw0G006200 Rw0G010380 Rw0G018260 Rw1G016250 Rw1G017620 Rw1G018280 Rw1G018390 Rw2G002470 Rw2G008750 Rw2G028260 Rw2G033640 Rw2G033840 Rw4G000510 Rw4G003170 Rw4G004830 Rw4G006220 Rw4G010290 Rw5G009180 Rw5G009680 Rw5G015760 Rw5G017730 Rw5G021440 Rw5G035270 Rw5G042770 Rw6G002880 Rw6G003090 Rw6G003400 Rw6G007620 Rw6G011710 Rw6G017000 Rw6G029770 Rw7G009660 Rw7G020940 Rw7G032200 Rw7G034460 Rw7G034840 Rw7G038460

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2075
AatII GACGTC 1 cut(s) 1450
Acc36I ACCTGC 2 cut(s) 1987, 2075
AccB1I GGYRCC 1 cut(s) 2087
AccBSI CCGCTC 2 cut(s) 180, 1090
AccI GTMKAC 2 cut(s) 189, 2116
AccIII TCCGGA 1 cut(s) 2253
AciI CCGC 7 cut(s) 18, 138, 178, 296, 1090, 1240, 1291
AclWI GGATC 5 cut(s) 486, 1618, 2256, 2312, 2352
AcsI RAATTY 6 cut(s) 365, 493, 622, 960, 1000, 1362
AcuI CTGAAG 1 cut(s) 1266
AcyI GRCGYC 1 cut(s) 1447
AdeI CACNNNGTG 1 cut(s) 2363
AfaI GTAC 4 cut(s) 1313, 1340, 1516, 2442
AfiI CCNNNNNNNGG 6 cut(s) 183, 184, 210, 841, 2170, 2535
AflII CTTAAG 1 cut(s) 1547
AflIII ACRYGT 3 cut(s) 1029, 1335, 1689
AgeI ACCGGT 1 cut(s) 834
AgsI TTSAA 6 cut(s) 323, 331, 1172, 1750, 1976, 2315
AjnI CCWGG 5 cut(s) 789, 1763, 2090, 2290, 2533
AluBI AGCT 7 cut(s) 535, 585, 1904, 2100, 2153, 2170, 2371
AluI AGCT 7 cut(s) 535, 585, 1904, 2100, 2153, 2170, 2371
Alw21I GWGCWC 1 cut(s) 1681
Alw26I GTCTC 1 cut(s) 828
Alw44I GTGCAC 1 cut(s) 1677
AlwI GGATC 5 cut(s) 486, 1618, 2256, 2312, 2352
Ama87I CYCGRG 4 cut(s) 754, 875, 1255, 1342
Aor13HI TCCGGA 1 cut(s) 2253
AoxI GGCC 6 cut(s) 306, 315, 1152, 1868, 2241, 2515
ApaLI GTGCAC 1 cut(s) 1677
ApeKI GCWGC 8 cut(s) 506, 605, 1707, 1901, 2150, 2167, 2368, 2591
ApoI RAATTY 6 cut(s) 365, 493, 622, 960, 1000, 1362
AsiGI ACCGGT 1 cut(s) 834
Asp700I GAANNNNTTC 2 cut(s) 1943, 2674
AspS9I GGNCC 5 cut(s) 90, 213, 298, 316, 1159
AsuC2I CCSGG 2 cut(s) 211, 293
AsuHPI GGTGA 6 cut(s) 124, 382, 610, 910, 1753, 1940
AsuII TTCGAA 1 cut(s) 620
AvaI CYCGRG 4 cut(s) 754, 875, 1255, 1342
AvaII GGWCC 4 cut(s) 90, 213, 298, 1159
BaeGI GKGCMC 2 cut(s) 1681, 2490
BanI GGYRCC 1 cut(s) 2087
BarI GAAGNNNNNNTAC 2 cut(s) 1295, 1327
BauI CACGAG 1 cut(s) 1568
Bbv12I GWGCWC 1 cut(s) 1681
BbvI GCAGC 8 cut(s) 493, 592, 1694, 1913, 2162, 2179, 2380, 2603
BceAI ACGGC 4 cut(s) 293, 330, 1729, 1984
BcgI CGANNNNNNTGC 4 cut(s) 266, 300, 1207, 1241
BciT130I CCWGG 5 cut(s) 791, 1765, 2092, 2292, 2535
BciVI GTATCC 3 cut(s) 365, 743, 1280
BclI TGATCA 2 cut(s) 1422, 1758
BcnI CCSGG 2 cut(s) 211, 293
BcoDI GTCTC 1 cut(s) 828
BfaI CTAG 6 cut(s) 582, 1584, 1641, 1658, 1722, 2187
BfmI CTRYAG 2 cut(s) 1668, 2165
BfrI CTTAAG 1 cut(s) 1547
BfuAI ACCTGC 2 cut(s) 1987, 2075
BfuI GTATCC 3 cut(s) 365, 743, 1280
BglI GCCNNNNNGGC 2 cut(s) 293, 314
BisI GCNGC 8 cut(s) 507, 606, 1708, 1902, 2151, 2168, 2369, 2592
BlsI GCNGC 8 cut(s) 508, 607, 1709, 1903, 2152, 2169, 2370, 2593
Bme1390I CCNGG 7 cut(s) 211, 293, 791, 1765, 2092, 2292, 2535
Bme18I GGWCC 4 cut(s) 90, 213, 298, 1159
BmeT110I CYCGRG 4 cut(s) 754, 875, 1255, 1342
BmgT120I GGNCC 5 cut(s) 90, 213, 298, 316, 1159
BmiI GGNNCC 5 cut(s) 579, 1542, 2089, 2175, 2651
BmrFI CCNGG 7 cut(s) 211, 293, 791, 1765, 2092, 2292, 2535
BmrI ACTGGG 2 cut(s) 1005, 1527
BmsI GCATC 4 cut(s) 433, 1066, 1731, 2601
BmuI ACTGGG 2 cut(s) 1005, 1527
BplI GAGNNNNNCTC 2 cut(s) 569, 601
BpmI CTGGAG 3 cut(s) 1319, 2113, 2192
Bpu14I TTCGAA 1 cut(s) 620
BpuEI CTTGAG 1 cut(s) 287
BpuMI CCSGG 2 cut(s) 211, 293
BsaAI YACGTR 1 cut(s) 1315
BsaHI GRCGYC 1 cut(s) 1447
BsaJI CCNNGG 6 cut(s) 393, 1155, 1273, 1966, 2244, 2534
BsaWI WCCGGW 5 cut(s) 215, 263, 834, 861, 2253
BsaXI ACNNNNNCTCC 2 cut(s) 14, 44
Bsc4I CCNNNNNNNGG 6 cut(s) 183, 184, 210, 841, 2170, 2535
Bse118I RCCGGY 2 cut(s) 834, 1044
Bse1I ACTGG 6 cut(s) 1000, 1126, 1432, 1522, 1686, 2505
Bse3DI GCAATG 3 cut(s) 1119, 1887, 2511
BseAI TCCGGA 1 cut(s) 2253
BseBI CCWGG 5 cut(s) 791, 1765, 2092, 2292, 2535
BseDI CCNNGG 6 cut(s) 393, 1155, 1273, 1966, 2244, 2534
BseGI GGATG 6 cut(s) 448, 747, 1353, 1846, 1926, 2409
BseLI CCNNNNNNNGG 6 cut(s) 183, 184, 210, 841, 2170, 2535
BseMI GCAATG 3 cut(s) 1119, 1887, 2511
BseMII CTCAG 3 cut(s) 462, 839, 2061
BseNI ACTGG 6 cut(s) 1000, 1126, 1432, 1522, 1686, 2505
BseRI GAGGAG 6 cut(s) 17, 100, 794, 1163, 1818, 2510
BseSI GKGCMC 2 cut(s) 1681, 2490
BseXI GCAGC 8 cut(s) 493, 592, 1694, 1913, 2162, 2179, 2380, 2603
BseYI CCCAGC 1 cut(s) 2339
BsgI GTGCAG 2 cut(s) 591, 1965
BshFI GGCC 6 cut(s) 308, 317, 1154, 1870, 2243, 2517
BshNI GGYRCC 1 cut(s) 2087
BshTI ACCGGT 1 cut(s) 834
BsiHKAI GWGCWC 1 cut(s) 1681
BsiHKCI CYCGRG 4 cut(s) 754, 875, 1255, 1342
BsiSI CCGG 8 cut(s) 211, 216, 264, 293, 835, 862, 1045, 2254
BslFI GGGAC 1 cut(s) 2551
BslI CCNNNNNNNGG 6 cut(s) 183, 184, 210, 841, 2170, 2535
BsmAI GTCTC 1 cut(s) 828
BsmFI GGGAC 1 cut(s) 2551
BsmI GAATGC 2 cut(s) 1114, 1250
BsnI GGCC 6 cut(s) 308, 317, 1154, 1870, 2243, 2517
BsoBI CYCGRG 4 cut(s) 754, 875, 1255, 1342
Bsp119I TTCGAA 1 cut(s) 620
Bsp1286I GDGCHC 2 cut(s) 1681, 2490
Bsp13I TCCGGA 1 cut(s) 2253
Bsp143I GATC 8 cut(s) 478, 1422, 1623, 1758, 2248, 2304, 2344, 2625
Bsp19I CCATGG 2 cut(s) 1273, 2244
BspACI CCGC 7 cut(s) 18, 138, 178, 296, 1090, 1240, 1291
BspANI GGCC 6 cut(s) 308, 317, 1154, 1870, 2243, 2517
BspCNI CTCAG 3 cut(s) 463, 838, 2062
BspEI TCCGGA 1 cut(s) 2253
BspHI TCATGA 1 cut(s) 1450
BspLI GGNNCC 5 cut(s) 579, 1542, 2089, 2175, 2651
BspMAI CTGCAG 1 cut(s) 2169
BspMI ACCTGC 2 cut(s) 1987, 2075
BspPI GGATC 5 cut(s) 486, 1618, 2256, 2312, 2352
BspT104I TTCGAA 1 cut(s) 620
BspT107I GGYRCC 1 cut(s) 2087
BspTI CTTAAG 1 cut(s) 1547
BsrBI CCGCTC 2 cut(s) 180, 1090
BsrDI GCAATG 3 cut(s) 1119, 1887, 2511
BsrFI RCCGGY 2 cut(s) 834, 1044
BsrI ACTGG 6 cut(s) 1000, 1126, 1432, 1522, 1686, 2505
BssAI RCCGGY 2 cut(s) 834, 1044
BssECI CCNNGG 6 cut(s) 393, 1155, 1273, 1966, 2244, 2534
BssMI GATC 8 cut(s) 478, 1422, 1623, 1758, 2248, 2304, 2344, 2625
BssNI GRCGYC 1 cut(s) 1447
BssSI CACGAG 1 cut(s) 1568
BssT1I CCWWGG 3 cut(s) 1155, 1273, 2244
Bst2BI CACGAG 1 cut(s) 1568
Bst2UI CCWGG 5 cut(s) 791, 1765, 2092, 2292, 2535
Bst4CI ACNGT 4 cut(s) 394, 706, 822, 904
Bst6I CTCTTC 3 cut(s) 120, 1581, 1795
BstACI GRCGYC 1 cut(s) 1447
BstAFI CTTAAG 1 cut(s) 1547
BstAPI GCANNNNNTGC 1 cut(s) 1898
BstBAI YACGTR 1 cut(s) 1315
BstBI TTCGAA 1 cut(s) 620
BstC8I GCNNGC 5 cut(s) 537, 1046, 1230, 2040, 2102
BstDEI CTNAG 7 cut(s) 471, 732, 825, 1711, 1816, 2070, 2564
BstDSI CCRYGG 4 cut(s) 393, 1273, 1966, 2244
BstF5I GGATG 6 cut(s) 448, 747, 1353, 1846, 1926, 2409
BstKTI GATC 8 cut(s) 481, 1425, 1626, 1761, 2251, 2307, 2347, 2628
BstMAI GTCTC 1 cut(s) 828
BstMBI GATC 8 cut(s) 478, 1422, 1623, 1758, 2248, 2304, 2344, 2625
BstMWI GCNNNNNNNGC 8 cut(s) 293, 314, 545, 611, 655, 980, 1898, 2173
BstNI CCWGG 5 cut(s) 791, 1765, 2092, 2292, 2535
BstNSI RCATGY 5 cut(s) 927, 1033, 1236, 1339, 1693
BstSCI CCNGG 7 cut(s) 209, 291, 789, 1763, 2090, 2290, 2533
BstSFI CTRYAG 2 cut(s) 1668, 2165
BstSLI GKGCMC 2 cut(s) 1681, 2490
BstV1I GCAGC 8 cut(s) 493, 592, 1694, 1913, 2162, 2179, 2380, 2603
BstX2I RGATCY 3 cut(s) 1623, 2248, 2344
BstYI RGATCY 3 cut(s) 1623, 2248, 2344
BsuI GTATCC 3 cut(s) 365, 743, 1280
BsuRI GGCC 6 cut(s) 308, 317, 1154, 1870, 2243, 2517
BtgI CCRYGG 4 cut(s) 393, 1273, 1966, 2244
BtsCI GGATG 6 cut(s) 448, 747, 1353, 1846, 1926, 2409
BtsI GCAGTG 1 cut(s) 1021
BtsIMutI CAGTG 4 cut(s) 818, 1021, 1425, 1679
BveI ACCTGC 2 cut(s) 1987, 2075
Cac8I GCNNGC 5 cut(s) 537, 1046, 1230, 2040, 2102
CciI TCATGA 1 cut(s) 1450
Cfr10I RCCGGY 2 cut(s) 834, 1044
Cfr13I GGNCC 5 cut(s) 90, 213, 298, 316, 1159
CpoI CGGWCCG 1 cut(s) 213
CseI GACGC 1 cut(s) 1466
CsiI ACCWGGT 1 cut(s) 789
Csp6I GTAC 4 cut(s) 1312, 1339, 1515, 2441
CspAI ACCGGT 1 cut(s) 834
CspI CGGWCCG 1 cut(s) 213
CviQI GTAC 4 cut(s) 1312, 1339, 1515, 2441
DdeI CTNAG 7 cut(s) 471, 732, 825, 1711, 1816, 2070, 2564
DpnI GATC 8 cut(s) 480, 1424, 1625, 1760, 2250, 2306, 2346, 2627
DpnII GATC 8 cut(s) 478, 1422, 1623, 1758, 2248, 2304, 2344, 2625
DraIII CACNNNGTG 1 cut(s) 2363
Eam1104I CTCTTC 3 cut(s) 120, 1581, 1795
EarI CTCTTC 3 cut(s) 120, 1581, 1795
EciI GGCGGA 1 cut(s) 7
Eco130I CCWWGG 3 cut(s) 1155, 1273, 2244
Eco147I AGGCCT 2 cut(s) 1154, 1870
Eco47I GGWCC 4 cut(s) 90, 213, 298, 1159
Eco57I CTGAAG 1 cut(s) 1266
Eco88I CYCGRG 4 cut(s) 754, 875, 1255, 1342
EcoRI GAATTC 2 cut(s) 493, 1000
EcoRII CCWGG 5 cut(s) 789, 1763, 2090, 2290, 2533
EcoT14I CCWWGG 3 cut(s) 1155, 1273, 2244
EcoT22I ATGCAT 2 cut(s) 976, 1797
ErhI CCWWGG 3 cut(s) 1155, 1273, 2244
FaqI GGGAC 1 cut(s) 2551
FauI CCCGC 1 cut(s) 185
FauNDI CATATG 1 cut(s) 2044
FbaI TGATCA 2 cut(s) 1422, 1758
FblI GTMKAC 2 cut(s) 189, 2116
Fnu4HI GCNGC 8 cut(s) 507, 606, 1708, 1902, 2151, 2168, 2369, 2592
FokI GGATG 6 cut(s) 455, 754, 1360, 1853, 1933, 2416
Fsp4HI GCNGC 8 cut(s) 507, 606, 1708, 1902, 2151, 2168, 2369, 2592
FspBI CTAG 6 cut(s) 582, 1584, 1641, 1658, 1722, 2187
GluI GCNGC 8 cut(s) 507, 606, 1708, 1902, 2151, 2168, 2369, 2592
GsaI CCCAGC 1 cut(s) 2343
GsuI CTGGAG 3 cut(s) 1319, 2113, 2192
HaeIII GGCC 6 cut(s) 308, 317, 1154, 1870, 2243, 2517
HapII CCGG 8 cut(s) 211, 216, 264, 293, 835, 862, 1045, 2254
HgaI GACGC 1 cut(s) 1466
Hin1I GRCGYC 1 cut(s) 1447
HincII GTYRAC 5 cut(s) 190, 993, 1027, 1687, 2117
HindII GTYRAC 5 cut(s) 190, 993, 1027, 1687, 2117
HinfI GANTC 8 cut(s) 377, 689, 871, 882, 1294, 1573, 2118, 2568
HpaI GTTAAC 1 cut(s) 993
HpaII CCGG 8 cut(s) 211, 216, 264, 293, 835, 862, 1045, 2254
HphI GGTGA 6 cut(s) 124, 382, 610, 910, 1753, 1940
Hpy166II GTNNAC 9 cut(s) 190, 591, 818, 993, 1027, 1679, 1687, 2117, 2441
Hpy188I TCNGA 9 cut(s) 568, 828, 887, 1246, 1369, 1740, 1938, 2071, 2398
Hpy8I GTNNAC 9 cut(s) 190, 591, 818, 993, 1027, 1679, 1687, 2117, 2441
Hpy99I CGWCG 1 cut(s) 1068
HpyCH4III ACNGT 4 cut(s) 394, 706, 822, 904
HpyCH4IV ACGT 2 cut(s) 1314, 1447
HpyF10VI GCNNNNNNNGC 8 cut(s) 293, 314, 545, 611, 655, 980, 1898, 2173
HpyF3I CTNAG 7 cut(s) 471, 732, 825, 1711, 1816, 2070, 2564
HpySE526I ACGT 2 cut(s) 1314, 1447
Hsp92I GRCGYC 1 cut(s) 1447
Kpn2I TCCGGA 1 cut(s) 2253
KroI GCCGGC 1 cut(s) 1044
KroNI GCCGGC 1 cut(s) 1046
Ksp22I TGATCA 2 cut(s) 1422, 1758
KspAI GTTAAC 1 cut(s) 993
Kzo9I GATC 8 cut(s) 478, 1422, 1623, 1758, 2248, 2304, 2344, 2625
LmnI GCTCC 7 cut(s) 206, 532, 577, 1087, 2035, 2173, 2376
Lsp1109I GCAGC 8 cut(s) 493, 592, 1694, 1913, 2162, 2179, 2380, 2603
LweI GCATC 4 cut(s) 433, 1066, 1731, 2601
MabI ACCWGGT 1 cut(s) 789
MaeI CTAG 6 cut(s) 582, 1584, 1641, 1658, 1722, 2187
MaeII ACGT 2 cut(s) 1314, 1447
MaeIII GTNAC 8 cut(s) 218, 310, 388, 785, 898, 1570, 1879, 2189
MalI GATC 8 cut(s) 480, 1424, 1625, 1760, 2250, 2306, 2346, 2627
MbiI CCGCTC 2 cut(s) 180, 1090
MboI GATC 8 cut(s) 478, 1422, 1623, 1758, 2248, 2304, 2344, 2625
MflI RGATCY 3 cut(s) 1623, 2248, 2344
MhlI GDGCHC 2 cut(s) 1681, 2490
MluCI AATT 9 cut(s) 365, 493, 622, 960, 1000, 1362, 1893, 2145, 2671
MlyI GAGTC 6 cut(s) 386, 698, 1303, 1567, 2112, 2577
MmeI TCCRAC 3 cut(s) 91, 924, 2282
Mph1103I ATGCAT 2 cut(s) 976, 1797
MroI TCCGGA 1 cut(s) 2253
MroNI GCCGGC 1 cut(s) 1044
MroXI GAANNNNTTC 2 cut(s) 1943, 2674
MseI TTAA 6 cut(s) 992, 1380, 1464, 1548, 1734, 2633
MslI CAYNNNNRTG 6 cut(s) 251, 354, 969, 1211, 1632, 2127
MspA1I CMGCKG 1 cut(s) 2170
MspCI CTTAAG 1 cut(s) 1547
MspI CCGG 8 cut(s) 211, 216, 264, 293, 835, 862, 1045, 2254
MspR9I CCNGG 7 cut(s) 211, 293, 791, 1765, 2092, 2292, 2535
Mva1269I GAATGC 2 cut(s) 1114, 1250
MvaI CCWGG 5 cut(s) 791, 1765, 2092, 2292, 2535
MwoI GCNNNNNNNGC 8 cut(s) 293, 314, 545, 611, 655, 980, 1898, 2173
NaeI GCCGGC 1 cut(s) 1046
NciI CCSGG 2 cut(s) 211, 293
NcoI CCATGG 2 cut(s) 1273, 2244
NdeI CATATG 1 cut(s) 2044
NdeII GATC 8 cut(s) 478, 1422, 1623, 1758, 2248, 2304, 2344, 2625
NgoMIV GCCGGC 1 cut(s) 1044
NlaIV GGNNCC 5 cut(s) 579, 1542, 2089, 2175, 2651
NmuCI GTSAC 7 cut(s) 218, 310, 388, 785, 898, 1570, 2189
NsiI ATGCAT 2 cut(s) 976, 1797
NspI RCATGY 5 cut(s) 927, 1033, 1236, 1339, 1693
NspV TTCGAA 1 cut(s) 620
PaeR7I CTCGAG 3 cut(s) 754, 875, 1342
PagI TCATGA 1 cut(s) 1450
PaqCI CACCTGC 1 cut(s) 2075
PceI AGGCCT 2 cut(s) 1154, 1870
PciI ACATGT 3 cut(s) 1029, 1335, 1689
PctI GAATGC 2 cut(s) 1114, 1250
PdiI GCCGGC 1 cut(s) 1046
PdmI GAANNNNTTC 2 cut(s) 1943, 2674
PfeI GAWTC 2 cut(s) 871, 882
PinAI ACCGGT 1 cut(s) 834
PkrI GCNGC 8 cut(s) 508, 607, 1709, 1903, 2152, 2169, 2370, 2593
PleI GAGTC 6 cut(s) 385, 697, 1302, 1567, 2112, 2576
PpsI GAGTC 6 cut(s) 385, 697, 1302, 1567, 2112, 2576
Ppu21I YACGTR 1 cut(s) 1315
PscI ACATGT 3 cut(s) 1029, 1335, 1689
Psp6I CCWGG 5 cut(s) 789, 1763, 2090, 2290, 2533
PspFI CCCAGC 1 cut(s) 2339
PspGI CCWGG 5 cut(s) 789, 1763, 2090, 2290, 2533
PspN4I GGNNCC 5 cut(s) 579, 1542, 2089, 2175, 2651
PspPI GGNCC 5 cut(s) 90, 213, 298, 316, 1159
PspXI VCTCGAGB 1 cut(s) 1342
PstI CTGCAG 1 cut(s) 2169
PsuI RGATCY 3 cut(s) 1623, 2248, 2344
PvuII CAGCTG 1 cut(s) 2170
RsaI GTAC 4 cut(s) 1313, 1340, 1516, 2442
RsaNI GTAC 4 cut(s) 1312, 1339, 1515, 2441
RseI CAYNNNNRTG 6 cut(s) 251, 354, 969, 1211, 1632, 2127
Rsr2I CGGWCCG 1 cut(s) 213
RsrII CGGWCCG 1 cut(s) 213
SalI GTCGAC 2 cut(s) 188, 2115
SaqAI TTAA 6 cut(s) 992, 1380, 1464, 1548, 1734, 2633
SatI GCNGC 8 cut(s) 507, 606, 1708, 1902, 2151, 2168, 2369, 2592
Sau3AI GATC 8 cut(s) 478, 1422, 1623, 1758, 2248, 2304, 2344, 2625
Sau96I GGNCC 5 cut(s) 90, 213, 298, 316, 1159
SchI GAGTC 6 cut(s) 386, 698, 1303, 1567, 2112, 2577
ScrFI CCNGG 7 cut(s) 211, 293, 791, 1765, 2092, 2292, 2535
SduI GDGCHC 2 cut(s) 1681, 2490
SexAI ACCWGGT 1 cut(s) 789
SfaNI GCATC 4 cut(s) 433, 1066, 1731, 2601
SfcI CTRYAG 2 cut(s) 1668, 2165
SfiI GGCCNNNNNGGCC 1 cut(s) 314
Sfr274I CTCGAG 3 cut(s) 754, 875, 1342
SfuI TTCGAA 1 cut(s) 620
SinI GGWCC 4 cut(s) 90, 213, 298, 1159
SlaI CTCGAG 3 cut(s) 754, 875, 1342
SmiMI CAYNNNNRTG 6 cut(s) 251, 354, 969, 1211, 1632, 2127
SmlI CTYRAG 5 cut(s) 302, 754, 875, 1342, 1547
SmoI CTYRAG 5 cut(s) 302, 754, 875, 1342, 1547
Sse9I AATT 9 cut(s) 365, 493, 622, 960, 1000, 1362, 1893, 2145, 2671
SseBI AGGCCT 2 cut(s) 1154, 1870
SsiI CCGC 7 cut(s) 18, 138, 178, 296, 1090, 1240, 1291
SspMI CTAG 6 cut(s) 582, 1584, 1641, 1658, 1722, 2187
StuI AGGCCT 2 cut(s) 1154, 1870
StyD4I CCNGG 7 cut(s) 209, 291, 789, 1763, 2090, 2290, 2533
StyI CCWWGG 3 cut(s) 1155, 1273, 2244
TaaI ACNGT 4 cut(s) 394, 706, 822, 904
TaiI ACGT 2 cut(s) 1317, 1450
TasI AATT 9 cut(s) 365, 493, 622, 960, 1000, 1362, 1893, 2145, 2671
TatI WGTACW 2 cut(s) 1338, 2440
TfiI GAWTC 2 cut(s) 871, 882
Tru1I TTAA 6 cut(s) 992, 1380, 1464, 1548, 1734, 2633
Tru9I TTAA 6 cut(s) 992, 1380, 1464, 1548, 1734, 2633
TscAI CASTG 4 cut(s) 825, 1021, 1432, 1686
TseFI GTSAC 7 cut(s) 218, 310, 388, 785, 898, 1570, 2189
TseI GCWGC 8 cut(s) 506, 605, 1707, 1901, 2150, 2167, 2368, 2591
Tsp45I GTSAC 7 cut(s) 218, 310, 388, 785, 898, 1570, 2189
TspRI CASTG 4 cut(s) 825, 1021, 1432, 1686
Vha464I CTTAAG 1 cut(s) 1547
VneI GTGCAC 1 cut(s) 1677
VpaK11BI GGWCC 4 cut(s) 90, 213, 298, 1159
XapI RAATTY 6 cut(s) 365, 493, 622, 960, 1000, 1362
XbaI TCTAGA 1 cut(s) 1721
XceI RCATGY 5 cut(s) 927, 1033, 1236, 1339, 1693
XhoI CTCGAG 3 cut(s) 754, 875, 1342
XmiI GTMKAC 2 cut(s) 189, 2116
XmnI GAANNNNTTC 2 cut(s) 1943, 2674
XspI CTAG 6 cut(s) 582, 1584, 1641, 1658, 1722, 2187
ZraI GACGTC 1 cut(s) 1448
Zsp2I ATGCAT 2 cut(s) 976, 1797
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.