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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 55 |
| AatII | GACGTC | 1 cut(s) | 531 |
| Acc36I | ACCTGC | 1 cut(s) | 55 |
| AccB1I | GGYRCC | 1 cut(s) | 760 |
| AciI | CCGC | 5 cut(s) | 37, 134, 145, 148, 309 |
| AclWI | GGATC | 1 cut(s) | 175 |
| AcsI | RAATTY | 3 cut(s) | 50, 322, 595 |
| AcvI | CACGTG | 1 cut(s) | 367 |
| AcyI | GRCGYC | 1 cut(s) | 528 |
| AdeI | CACNNNGTG | 1 cut(s) | 662 |
| AfaI | GTAC | 1 cut(s) | 441 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 17, 164, 413 |
| AgsI | TTSAA | 2 cut(s) | 328, 690 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 287 |
| AjiI | CACGTC | 1 cut(s) | 662 |
| AjnI | CCWGG | 1 cut(s) | 499 |
| Alw26I | GTCTC | 2 cut(s) | 295, 572 |
| AlwI | GGATC | 1 cut(s) | 175 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 701 |
| Ama87I | CYCGRG | 1 cut(s) | 292 |
| AoxI | GGCC | 2 cut(s) | 404, 502 |
| ApoI | RAATTY | 3 cut(s) | 50, 322, 595 |
| AspLEI | GCGC | 1 cut(s) | 247 |
| AspS9I | GGNCC | 1 cut(s) | 404 |
| AsuC2I | CCSGG | 1 cut(s) | 11 |
| AsuHPI | GGTGA | 1 cut(s) | 623 |
| AvaI | CYCGRG | 1 cut(s) | 292 |
| BanI | GGYRCC | 1 cut(s) | 760 |
| BanII | GRGCYC | 2 cut(s) | 10, 431 |
| BbrPI | CACGTG | 1 cut(s) | 367 |
| BccI | CCATC | 4 cut(s) | 52, 89, 380, 674 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 236, 270 |
| BciT130I | CCWGG | 1 cut(s) | 501 |
| BciVI | GTATCC | 1 cut(s) | 772 |
| BcnI | CCSGG | 1 cut(s) | 11 |
| BcoDI | GTCTC | 2 cut(s) | 295, 572 |
| BfaI | CTAG | 1 cut(s) | 378 |
| BfuAI | ACCTGC | 1 cut(s) | 55 |
| BfuI | GTATCC | 1 cut(s) | 772 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 14 |
| BisI | GCNGC | 1 cut(s) | 146 |
| BlpI | GCTNAGC | 1 cut(s) | 141 |
| BlsI | GCNGC | 1 cut(s) | 147 |
| Bme1390I | CCNGG | 2 cut(s) | 11, 501 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 287 |
| BmeT110I | CYCGRG | 1 cut(s) | 292 |
| BmgBI | CACGTC | 1 cut(s) | 662 |
| BmgT120I | GGNCC | 1 cut(s) | 404 |
| BmiI | GGNNCC | 1 cut(s) | 762 |
| BmrFI | CCNGG | 2 cut(s) | 11, 501 |
| BmrI | ACTGGG | 1 cut(s) | 431 |
| BmuI | ACTGGG | 1 cut(s) | 431 |
| BoxI | GACNNNNGTC | 1 cut(s) | 652 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 141 |
| BpuMI | CCSGG | 1 cut(s) | 11 |
| BsaAI | YACGTR | 1 cut(s) | 367 |
| BsaBI | GATNNNNATC | 1 cut(s) | 561 |
| BsaHI | GRCGYC | 1 cut(s) | 528 |
| BsaJI | CCNNGG | 2 cut(s) | 9, 640 |
| BsaWI | WCCGGW | 1 cut(s) | 157 |
| BsaXI | ACNNNNNCTCC | 4 cut(s) | 66, 96, 658, 688 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 17, 164, 413 |
| Bse1I | ACTGG | 1 cut(s) | 437 |
| Bse8I | GATNNNNATC | 1 cut(s) | 561 |
| BseBI | CCWGG | 1 cut(s) | 501 |
| BseDI | CCNNGG | 2 cut(s) | 9, 640 |
| BseGI | GGATG | 2 cut(s) | 265, 685 |
| BseJI | GATNNNNATC | 1 cut(s) | 561 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 17, 164, 413 |
| BseMII | CTCAG | 3 cut(s) | 444, 567, 737 |
| BseNI | ACTGG | 1 cut(s) | 437 |
| BseRI | GAGGAG | 2 cut(s) | 176, 444 |
| BseYI | CCCAGC | 2 cut(s) | 17, 701 |
| BshFI | GGCC | 2 cut(s) | 406, 504 |
| BshNI | GGYRCC | 1 cut(s) | 760 |
| BsiHKCI | CYCGRG | 1 cut(s) | 292 |
| BsiSI | CCGG | 2 cut(s) | 11, 158 |
| BslFI | GGGAC | 2 cut(s) | 122, 294 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 17, 164, 413 |
| BsmAI | GTCTC | 2 cut(s) | 295, 572 |
| BsmFI | GGGAC | 2 cut(s) | 122, 294 |
| BsnI | GGCC | 2 cut(s) | 406, 504 |
| BsoBI | CYCGRG | 1 cut(s) | 292 |
| Bsp1286I | GDGCHC | 2 cut(s) | 10, 431 |
| Bsp143I | GATC | 1 cut(s) | 167 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 141 |
| Bsp19I | CCATGG | 1 cut(s) | 640 |
| BspACI | CCGC | 5 cut(s) | 37, 134, 145, 148, 309 |
| BspANI | GGCC | 2 cut(s) | 406, 504 |
| BspCNI | CTCAG | 3 cut(s) | 443, 568, 736 |
| BspLI | GGNNCC | 1 cut(s) | 762 |
| BspMI | ACCTGC | 1 cut(s) | 55 |
| BspPI | GGATC | 1 cut(s) | 175 |
| BspT107I | GGYRCC | 1 cut(s) | 760 |
| BsrI | ACTGG | 1 cut(s) | 437 |
| BssECI | CCNNGG | 2 cut(s) | 9, 640 |
| BssMI | GATC | 1 cut(s) | 167 |
| BssNI | GRCGYC | 1 cut(s) | 528 |
| BssT1I | CCWWGG | 1 cut(s) | 640 |
| Bst2UI | CCWGG | 1 cut(s) | 501 |
| Bst4CI | ACNGT | 3 cut(s) | 523, 628, 757 |
| Bst6I | CTCTTC | 2 cut(s) | 729, 768 |
| BstACI | GRCGYC | 1 cut(s) | 528 |
| BstBAI | YACGTR | 1 cut(s) | 367 |
| BstC8I | GCNNGC | 4 cut(s) | 6, 138, 219, 311 |
| BstDEI | CTNAG | 5 cut(s) | 122, 141, 430, 576, 723 |
| BstDSI | CCRYGG | 1 cut(s) | 640 |
| BstF5I | GGATG | 2 cut(s) | 265, 685 |
| BstHHI | GCGC | 1 cut(s) | 247 |
| BstKTI | GATC | 1 cut(s) | 170 |
| BstMAI | GTCTC | 2 cut(s) | 295, 572 |
| BstMBI | GATC | 1 cut(s) | 167 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 14, 142, 151 |
| BstNI | CCWGG | 1 cut(s) | 501 |
| BstNSI | RCATGY | 2 cut(s) | 221, 446 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 652 |
| BstSCI | CCNGG | 2 cut(s) | 9, 499 |
| BsuI | GTATCC | 1 cut(s) | 772 |
| BsuRI | GGCC | 2 cut(s) | 406, 504 |
| BtgI | CCRYGG | 1 cut(s) | 640 |
| BtrI | CACGTC | 1 cut(s) | 662 |
| BtsCI | GGATG | 2 cut(s) | 265, 685 |
| BtsIMutI | CAGTG | 2 cut(s) | 528, 762 |
| BveI | ACCTGC | 1 cut(s) | 55 |
| Cac8I | GCNNGC | 4 cut(s) | 6, 138, 219, 311 |
| CaiI | CAGNNNCTG | 1 cut(s) | 701 |
| CfoI | GCGC | 1 cut(s) | 247 |
| Cfr13I | GGNCC | 1 cut(s) | 404 |
| CseI | GACGC | 1 cut(s) | 210 |
| Csp6I | GTAC | 1 cut(s) | 440 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 625, 660 |
| CviAII | CATG | 3 cut(s) | 218, 443, 641 |
| CviQI | GTAC | 1 cut(s) | 440 |
| DdeI | CTNAG | 5 cut(s) | 122, 141, 430, 576, 723 |
| DpnI | GATC | 1 cut(s) | 169 |
| DpnII | GATC | 1 cut(s) | 167 |
| DraIII | CACNNNGTG | 1 cut(s) | 662 |
| DriI | GACNNNNNGTC | 1 cut(s) | 287 |
| Eam1104I | CTCTTC | 2 cut(s) | 729, 768 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 287 |
| EarI | CTCTTC | 2 cut(s) | 729, 768 |
| Eco130I | CCWWGG | 1 cut(s) | 640 |
| Eco24I | GRGCYC | 2 cut(s) | 10, 431 |
| Eco72I | CACGTG | 1 cut(s) | 367 |
| Eco88I | CYCGRG | 1 cut(s) | 292 |
| EcoRI | GAATTC | 1 cut(s) | 50 |
| EcoRII | CCWGG | 1 cut(s) | 499 |
| EcoT14I | CCWWGG | 1 cut(s) | 640 |
| EcoT38I | GRGCYC | 2 cut(s) | 10, 431 |
| ErhI | CCWWGG | 1 cut(s) | 640 |
| FaeI | CATG | 3 cut(s) | 221, 446, 644 |
| FaiI | YATR | 4 cut(s) | 86, 219, 444, 642 |
| FaqI | GGGAC | 2 cut(s) | 122, 294 |
| FatI | CATG | 3 cut(s) | 217, 442, 640 |
| FauI | CCCGC | 1 cut(s) | 302 |
| Fnu4HI | GCNGC | 1 cut(s) | 146 |
| FokI | GGATG | 2 cut(s) | 272, 692 |
| FriOI | GRGCYC | 2 cut(s) | 10, 431 |
| Fsp4HI | GCNGC | 1 cut(s) | 146 |
| FspBI | CTAG | 1 cut(s) | 378 |
| GlaI | GCGC | 1 cut(s) | 246 |
| GluI | GCNGC | 1 cut(s) | 146 |
| GsaI | CCCAGC | 2 cut(s) | 21, 705 |
| HaeIII | GGCC | 2 cut(s) | 406, 504 |
| HapII | CCGG | 2 cut(s) | 11, 158 |
| HgaI | GACGC | 1 cut(s) | 210 |
| HhaI | GCGC | 1 cut(s) | 247 |
| Hin1I | GRCGYC | 1 cut(s) | 528 |
| Hin1II | CATG | 3 cut(s) | 221, 446, 644 |
| Hin6I | GCGC | 1 cut(s) | 245 |
| HinP1I | GCGC | 1 cut(s) | 245 |
| HinfI | GANTC | 5 cut(s) | 101, 176, 449, 557, 653 |
| HpaII | CCGG | 2 cut(s) | 11, 158 |
| HphI | GGTGA | 1 cut(s) | 623 |
| Hpy166II | GTNNAC | 4 cut(s) | 347, 466, 526, 647 |
| Hpy188I | TCNGA | 5 cut(s) | 196, 226, 433, 459, 726 |
| Hpy188III | TCNNGA | 6 cut(s) | 55, 234, 257, 292, 333, 657 |
| Hpy8I | GTNNAC | 4 cut(s) | 347, 466, 526, 647 |
| Hpy99I | CGWCG | 1 cut(s) | 663 |
| HpyAV | CCTTC | 1 cut(s) | 763 |
| HpyCH4III | ACNGT | 3 cut(s) | 523, 628, 757 |
| HpyCH4IV | ACGT | 3 cut(s) | 366, 528, 661 |
| HpyCH4V | TGCA | 2 cut(s) | 353, 636 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 14, 142, 151 |
| HpyF3I | CTNAG | 5 cut(s) | 122, 141, 430, 576, 723 |
| HpySE526I | ACGT | 3 cut(s) | 366, 528, 661 |
| Hsp92I | GRCGYC | 1 cut(s) | 528 |
| Hsp92II | CATG | 3 cut(s) | 221, 446, 644 |
| HspAI | GCGC | 1 cut(s) | 245 |
| Kzo9I | GATC | 1 cut(s) | 167 |
| LmnI | GCTCC | 1 cut(s) | 61 |
| MaeI | CTAG | 1 cut(s) | 378 |
| MaeII | ACGT | 3 cut(s) | 366, 528, 661 |
| MaeIII | GTNAC | 2 cut(s) | 367, 654 |
| MalI | GATC | 1 cut(s) | 169 |
| MboI | GATC | 1 cut(s) | 167 |
| MboII | GAAGA | 4 cut(s) | 176, 566, 746, 785 |
| MhlI | GDGCHC | 2 cut(s) | 10, 431 |
| MluCI | AATT | 6 cut(s) | 50, 227, 264, 284, 322, 595 |
| MlyI | GAGTC | 3 cut(s) | 110, 185, 662 |
| MmeI | TCCRAC | 1 cut(s) | 52 |
| MseI | TTAA | 1 cut(s) | 267 |
| MspA1I | CMGCKG | 1 cut(s) | 136 |
| MspI | CCGG | 2 cut(s) | 11, 158 |
| MspR9I | CCNGG | 2 cut(s) | 11, 501 |
| MvaI | CCWGG | 1 cut(s) | 501 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 14, 142, 151 |
| NciI | CCSGG | 1 cut(s) | 11 |
| NcoI | CCATGG | 1 cut(s) | 640 |
| NdeII | GATC | 1 cut(s) | 167 |
| NlaIII | CATG | 3 cut(s) | 221, 446, 644 |
| NlaIV | GGNNCC | 1 cut(s) | 762 |
| NmuCI | GTSAC | 2 cut(s) | 367, 654 |
| NspI | RCATGY | 2 cut(s) | 221, 446 |
| PaeI | GCATGC | 1 cut(s) | 221 |
| PaeR7I | CTCGAG | 1 cut(s) | 292 |
| PaqCI | CACCTGC | 1 cut(s) | 55 |
| PfeI | GAWTC | 2 cut(s) | 449, 557 |
| PkrI | GCNGC | 1 cut(s) | 147 |
| PleI | GAGTC | 3 cut(s) | 109, 184, 661 |
| PmaCI | CACGTG | 1 cut(s) | 367 |
| PmlI | CACGTG | 1 cut(s) | 367 |
| PpsI | GAGTC | 3 cut(s) | 109, 184, 661 |
| Ppu21I | YACGTR | 1 cut(s) | 367 |
| PshAI | GACNNNNGTC | 1 cut(s) | 652 |
| Psp6I | CCWGG | 1 cut(s) | 499 |
| PspCI | CACGTG | 1 cut(s) | 367 |
| PspFI | CCCAGC | 2 cut(s) | 17, 701 |
| PspGI | CCWGG | 1 cut(s) | 499 |
| PspN4I | GGNNCC | 1 cut(s) | 762 |
| PspPI | GGNCC | 1 cut(s) | 404 |
| PstNI | CAGNNNCTG | 1 cut(s) | 701 |
| RsaI | GTAC | 1 cut(s) | 441 |
| RsaNI | GTAC | 1 cut(s) | 440 |
| SaqAI | TTAA | 1 cut(s) | 267 |
| SatI | GCNGC | 1 cut(s) | 146 |
| Sau3AI | GATC | 1 cut(s) | 167 |
| Sau96I | GGNCC | 1 cut(s) | 404 |
| SchI | GAGTC | 3 cut(s) | 110, 185, 662 |
| ScrFI | CCNGG | 2 cut(s) | 11, 501 |
| SduI | GDGCHC | 2 cut(s) | 10, 431 |
| Sfr274I | CTCGAG | 1 cut(s) | 292 |
| SlaI | CTCGAG | 1 cut(s) | 292 |
| SmlI | CTYRAG | 1 cut(s) | 292 |
| SmoI | CTYRAG | 1 cut(s) | 292 |
| SphI | GCATGC | 1 cut(s) | 221 |
| Sse9I | AATT | 6 cut(s) | 50, 227, 264, 284, 322, 595 |
| SsiI | CCGC | 5 cut(s) | 37, 134, 145, 148, 309 |
| SspMI | CTAG | 1 cut(s) | 378 |
| StyD4I | CCNGG | 2 cut(s) | 9, 499 |
| StyI | CCWWGG | 1 cut(s) | 640 |
| TaaI | ACNGT | 3 cut(s) | 523, 628, 757 |
| TaiI | ACGT | 3 cut(s) | 369, 531, 664 |
| TaqI | TCGA | 3 cut(s) | 54, 293, 552 |
| TasI | AATT | 6 cut(s) | 50, 227, 264, 284, 322, 595 |
| TatI | WGTACW | 1 cut(s) | 439 |
| TauI | GCSGC | 1 cut(s) | 148 |
| TfiI | GAWTC | 2 cut(s) | 449, 557 |
| Tru1I | TTAA | 1 cut(s) | 267 |
| Tru9I | TTAA | 1 cut(s) | 267 |
| TscAI | CASTG | 2 cut(s) | 528, 762 |
| TseFI | GTSAC | 2 cut(s) | 367, 654 |
| Tsp45I | GTSAC | 2 cut(s) | 367, 654 |
| TspDTI | ATGAA | 2 cut(s) | 276, 630 |
| TspGWI | ACGGA | 2 cut(s) | 188, 746 |
| TspRI | CASTG | 2 cut(s) | 528, 762 |
| XapI | RAATTY | 3 cut(s) | 50, 322, 595 |
| XceI | RCATGY | 2 cut(s) | 221, 446 |
| XhoI | CTCGAG | 1 cut(s) | 292 |
| XspI | CTAG | 1 cut(s) | 378 |
| ZraI | GACGTC | 1 cut(s) | 529 |