ribonuclease H protein
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 621, 699 |
| Acc36I | ACCTGC | 2 cut(s) | 621, 699 |
| AccII | CGCG | 1 cut(s) | 530 |
| AciI | CCGC | 2 cut(s) | 203, 530 |
| AclWI | GGATC | 6 cut(s) | 234, 267, 461, 474, 560, 573 |
| AfaI | GTAC | 1 cut(s) | 207 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 329 |
| AgsI | TTSAA | 1 cut(s) | 602 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 85 |
| AjnI | CCWGG | 1 cut(s) | 568 |
| AluBI | AGCT | 2 cut(s) | 498, 815 |
| AluI | AGCT | 2 cut(s) | 498, 815 |
| Alw21I | GWGCWC | 1 cut(s) | 612 |
| Alw26I | GTCTC | 4 cut(s) | 93, 277, 632, 663 |
| AlwI | GGATC | 6 cut(s) | 234, 267, 461, 474, 560, 573 |
| Ama87I | CYCGRG | 1 cut(s) | 343 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 721 |
| AspS9I | GGNCC | 1 cut(s) | 79 |
| AsuC2I | CCSGG | 2 cut(s) | 344, 345 |
| AsuHPI | GGTGA | 2 cut(s) | 397, 494 |
| AsuII | TTCGAA | 1 cut(s) | 160 |
| AvaI | CYCGRG | 1 cut(s) | 343 |
| AvaII | GGWCC | 1 cut(s) | 79 |
| BamHI | GGATCC | 2 cut(s) | 466, 565 |
| Bbv12I | GWGCWC | 1 cut(s) | 612 |
| BccI | CCATC | 4 cut(s) | 323, 328, 415, 479 |
| BceAI | ACGGC | 1 cut(s) | 697 |
| BciT130I | CCWGG | 1 cut(s) | 570 |
| BclI | TGATCA | 1 cut(s) | 93 |
| BcnI | CCSGG | 2 cut(s) | 344, 345 |
| BcoDI | GTCTC | 4 cut(s) | 93, 277, 632, 663 |
| BfaI | CTAG | 3 cut(s) | 363, 593, 818 |
| BfuAI | ACCTGC | 2 cut(s) | 621, 699 |
| Bme1390I | CCNGG | 3 cut(s) | 344, 345, 570 |
| Bme18I | GGWCC | 1 cut(s) | 79 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 85 |
| BmeT110I | CYCGRG | 1 cut(s) | 343 |
| BmgT120I | GGNCC | 1 cut(s) | 79 |
| BmiI | GGNNCC | 4 cut(s) | 321, 468, 534, 567 |
| BmrFI | CCNGG | 3 cut(s) | 344, 345, 570 |
| BmrI | ACTGGG | 1 cut(s) | 141 |
| BmuI | ACTGGG | 1 cut(s) | 141 |
| BoxI | GACNNNNGTC | 1 cut(s) | 289 |
| Bpu14I | TTCGAA | 1 cut(s) | 160 |
| BpuMI | CCSGG | 2 cut(s) | 344, 345 |
| BsaI | GGTCTC | 3 cut(s) | 93, 277, 663 |
| BsaJI | CCNNGG | 3 cut(s) | 287, 343, 569 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 329 |
| Bse1I | ACTGG | 2 cut(s) | 136, 213 |
| BseBI | CCWGG | 1 cut(s) | 570 |
| BseDI | CCNNGG | 3 cut(s) | 287, 343, 569 |
| BseGI | GGATG | 3 cut(s) | 52, 339, 555 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 329 |
| BseNI | ACTGG | 2 cut(s) | 136, 213 |
| BseRI | GAGGAG | 1 cut(s) | 710 |
| Bsh1236I | CGCG | 1 cut(s) | 530 |
| BsiHKAI | GWGCWC | 1 cut(s) | 612 |
| BsiHKCI | CYCGRG | 1 cut(s) | 343 |
| BsiSI | CCGG | 1 cut(s) | 344 |
| BslFI | GGGAC | 1 cut(s) | 587 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 329 |
| BsmAI | GTCTC | 4 cut(s) | 93, 277, 632, 663 |
| BsmFI | GGGAC | 1 cut(s) | 587 |
| Bso31I | GGTCTC | 3 cut(s) | 93, 277, 663 |
| BsoBI | CYCGRG | 1 cut(s) | 343 |
| Bsp119I | TTCGAA | 1 cut(s) | 160 |
| Bsp1286I | GDGCHC | 1 cut(s) | 612 |
| Bsp143I | GATC | 6 cut(s) | 93, 141, 226, 259, 466, 565 |
| BspACI | CCGC | 2 cut(s) | 203, 530 |
| BspFNI | CGCG | 1 cut(s) | 530 |
| BspLI | GGNNCC | 4 cut(s) | 321, 468, 534, 567 |
| BspMI | ACCTGC | 2 cut(s) | 621, 699 |
| BspPI | GGATC | 6 cut(s) | 234, 267, 461, 474, 560, 573 |
| BspT104I | TTCGAA | 1 cut(s) | 160 |
| BspTNI | GGTCTC | 3 cut(s) | 93, 277, 663 |
| BsrI | ACTGG | 2 cut(s) | 136, 213 |
| BssECI | CCNNGG | 3 cut(s) | 287, 343, 569 |
| BssMI | GATC | 6 cut(s) | 93, 141, 226, 259, 466, 565 |
| BssT1I | CCWWGG | 1 cut(s) | 287 |
| Bst2UI | CCWGG | 1 cut(s) | 570 |
| Bst4CI | ACNGT | 4 cut(s) | 475, 482, 582, 665 |
| BstAPI | GCANNNNNTGC | 1 cut(s) | 200 |
| BstBI | TTCGAA | 1 cut(s) | 160 |
| BstDEI | CTNAG | 2 cut(s) | 503, 539 |
| BstF5I | GGATG | 3 cut(s) | 52, 339, 555 |
| BstFNI | CGCG | 1 cut(s) | 530 |
| BstKTI | GATC | 6 cut(s) | 96, 144, 229, 262, 469, 568 |
| BstMAI | GTCTC | 4 cut(s) | 93, 277, 632, 663 |
| BstMBI | GATC | 6 cut(s) | 93, 141, 226, 259, 466, 565 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 200 |
| BstNI | CCWGG | 1 cut(s) | 570 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 289 |
| BstSCI | CCNGG | 3 cut(s) | 342, 343, 568 |
| BstUI | CGCG | 1 cut(s) | 530 |
| BstX2I | RGATCY | 2 cut(s) | 466, 565 |
| BstYI | RGATCY | 2 cut(s) | 466, 565 |
| BtsCI | GGATG | 3 cut(s) | 52, 339, 555 |
| BtsI | GCAGTG | 1 cut(s) | 305 |
| BtsIMutI | CAGTG | 4 cut(s) | 129, 305, 661, 832 |
| BveI | ACCTGC | 2 cut(s) | 621, 699 |
| Cfr13I | GGNCC | 1 cut(s) | 79 |
| Cfr9I | CCCGGG | 1 cut(s) | 343 |
| Csp6I | GTAC | 1 cut(s) | 206 |
| CviAII | CATG | 4 cut(s) | 58, 457, 650, 752 |
| CviJI | RGCY | 5 cut(s) | 112, 320, 498, 535, 815 |
| CviKI_1 | RGCY | 5 cut(s) | 112, 320, 498, 535, 815 |
| CviQI | GTAC | 1 cut(s) | 206 |
| DdeI | CTNAG | 2 cut(s) | 503, 539 |
| DpnI | GATC | 6 cut(s) | 95, 143, 228, 261, 468, 567 |
| DpnII | GATC | 6 cut(s) | 93, 141, 226, 259, 466, 565 |
| DraI | TTTAAA | 1 cut(s) | 247 |
| DriI | GACNNNNNGTC | 1 cut(s) | 85 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 85 |
| Eco130I | CCWWGG | 1 cut(s) | 287 |
| Eco31I | GGTCTC | 3 cut(s) | 93, 277, 663 |
| Eco47I | GGWCC | 1 cut(s) | 79 |
| Eco88I | CYCGRG | 1 cut(s) | 343 |
| EcoRII | CCWGG | 1 cut(s) | 568 |
| EcoT14I | CCWWGG | 1 cut(s) | 287 |
| ErhI | CCWWGG | 1 cut(s) | 287 |
| FaeI | CATG | 4 cut(s) | 61, 460, 653, 755 |
| FaiI | YATR | 8 cut(s) | 59, 167, 181, 458, 606, 651, 705, 753 |
| FaqI | GGGAC | 1 cut(s) | 587 |
| FatI | CATG | 4 cut(s) | 57, 456, 649, 751 |
| FbaI | TGATCA | 1 cut(s) | 93 |
| FokI | GGATG | 3 cut(s) | 59, 346, 562 |
| FspBI | CTAG | 3 cut(s) | 363, 593, 818 |
| HapII | CCGG | 1 cut(s) | 344 |
| Hin1II | CATG | 4 cut(s) | 61, 460, 653, 755 |
| HincII | GTYRAC | 5 cut(s) | 186, 340, 462, 585, 690 |
| HindII | GTYRAC | 5 cut(s) | 186, 340, 462, 585, 690 |
| HinfI | GANTC | 1 cut(s) | 525 |
| HpaI | GTTAAC | 1 cut(s) | 690 |
| HpaII | CCGG | 1 cut(s) | 344 |
| HphI | GGTGA | 2 cut(s) | 397, 494 |
| Hpy166II | GTNNAC | 7 cut(s) | 186, 340, 462, 478, 585, 638, 690 |
| Hpy188I | TCNGA | 3 cut(s) | 93, 253, 328 |
| Hpy188III | TCNNGA | 4 cut(s) | 44, 172, 644, 818 |
| Hpy8I | GTNNAC | 7 cut(s) | 186, 340, 462, 478, 585, 638, 690 |
| Hpy99I | CGWCG | 1 cut(s) | 33 |
| HpyAV | CCTTC | 3 cut(s) | 115, 184, 420 |
| HpyCH4III | ACNGT | 4 cut(s) | 475, 482, 582, 665 |
| HpyCH4IV | ACGT | 2 cut(s) | 9, 147 |
| HpyCH4V | TGCA | 5 cut(s) | 277, 404, 559, 751, 763 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 200 |
| HpyF3I | CTNAG | 2 cut(s) | 503, 539 |
| HpySE526I | ACGT | 2 cut(s) | 9, 147 |
| Hsp92II | CATG | 4 cut(s) | 61, 460, 653, 755 |
| Ksp22I | TGATCA | 1 cut(s) | 93 |
| KspAI | GTTAAC | 1 cut(s) | 690 |
| Kzo9I | GATC | 6 cut(s) | 93, 141, 226, 259, 466, 565 |
| LmnI | GCTCC | 2 cut(s) | 325, 532 |
| MaeI | CTAG | 3 cut(s) | 363, 593, 818 |
| MaeII | ACGT | 2 cut(s) | 9, 147 |
| MaeIII | GTNAC | 2 cut(s) | 264, 519 |
| MalI | GATC | 6 cut(s) | 95, 143, 228, 261, 468, 567 |
| MboI | GATC | 6 cut(s) | 93, 141, 226, 259, 466, 565 |
| MboII | GAAGA | 2 cut(s) | 148, 151 |
| MfeI | CAATTG | 1 cut(s) | 758 |
| MflI | RGATCY | 2 cut(s) | 466, 565 |
| MhlI | GDGCHC | 1 cut(s) | 612 |
| MluCI | AATT | 3 cut(s) | 301, 432, 758 |
| MlyI | GAGTC | 1 cut(s) | 519 |
| MmeI | TCCRAC | 2 cut(s) | 494, 543 |
| MnlI | CCTC | 5 cut(s) | 70, 78, 408, 546, 688 |
| MroXI | GAANNNNTTC | 1 cut(s) | 721 |
| MseI | TTAA | 4 cut(s) | 246, 359, 689, 810 |
| MspI | CCGG | 1 cut(s) | 344 |
| MspR9I | CCNGG | 3 cut(s) | 344, 345, 570 |
| MunI | CAATTG | 1 cut(s) | 758 |
| MvaI | CCWGG | 1 cut(s) | 570 |
| MvnI | CGCG | 1 cut(s) | 530 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 200 |
| NciI | CCSGG | 2 cut(s) | 344, 345 |
| NdeII | GATC | 6 cut(s) | 93, 141, 226, 259, 466, 565 |
| NlaIII | CATG | 4 cut(s) | 61, 460, 653, 755 |
| NlaIV | GGNNCC | 4 cut(s) | 321, 468, 534, 567 |
| NspV | TTCGAA | 1 cut(s) | 160 |
| PaqCI | CACCTGC | 2 cut(s) | 621, 699 |
| PdmI | GAANNNNTTC | 1 cut(s) | 721 |
| PleI | GAGTC | 1 cut(s) | 519 |
| PpsI | GAGTC | 1 cut(s) | 519 |
| PshAI | GACNNNNGTC | 1 cut(s) | 289 |
| Psp6I | CCWGG | 1 cut(s) | 568 |
| PspGI | CCWGG | 1 cut(s) | 568 |
| PspN4I | GGNNCC | 4 cut(s) | 321, 468, 534, 567 |
| PspPI | GGNCC | 1 cut(s) | 79 |
| PsuI | RGATCY | 2 cut(s) | 466, 565 |
| RsaI | GTAC | 1 cut(s) | 207 |
| RsaNI | GTAC | 1 cut(s) | 206 |
| SaqAI | TTAA | 4 cut(s) | 246, 359, 689, 810 |
| Sau3AI | GATC | 6 cut(s) | 93, 141, 226, 259, 466, 565 |
| Sau96I | GGNCC | 1 cut(s) | 79 |
| SchI | GAGTC | 1 cut(s) | 519 |
| ScrFI | CCNGG | 3 cut(s) | 344, 345, 570 |
| SduI | GDGCHC | 1 cut(s) | 612 |
| SfuI | TTCGAA | 1 cut(s) | 160 |
| SinI | GGWCC | 1 cut(s) | 79 |
| SmaI | CCCGGG | 1 cut(s) | 345 |
| Sse9I | AATT | 3 cut(s) | 301, 432, 758 |
| SsiI | CCGC | 2 cut(s) | 203, 530 |
| SspMI | CTAG | 3 cut(s) | 363, 593, 818 |
| StyD4I | CCNGG | 3 cut(s) | 342, 343, 568 |
| StyI | CCWWGG | 1 cut(s) | 287 |
| TaaI | ACNGT | 4 cut(s) | 475, 482, 582, 665 |
| TaiI | ACGT | 2 cut(s) | 12, 150 |
| TaqI | TCGA | 1 cut(s) | 160 |
| TasI | AATT | 3 cut(s) | 301, 432, 758 |
| Tru1I | TTAA | 4 cut(s) | 246, 359, 689, 810 |
| Tru9I | TTAA | 4 cut(s) | 246, 359, 689, 810 |
| TscAI | CASTG | 4 cut(s) | 136, 312, 668, 832 |
| TspDTI | ATGAA | 1 cut(s) | 445 |
| TspGWI | ACGGA | 4 cut(s) | 113, 312, 479, 707 |
| TspMI | CCCGGG | 1 cut(s) | 343 |
| TspRI | CASTG | 4 cut(s) | 136, 312, 668, 832 |
| VpaK11BI | GGWCC | 1 cut(s) | 79 |
| XbaI | TCTAGA | 1 cut(s) | 817 |
| XmaI | CCCGGG | 1 cut(s) | 343 |
| XmnI | GAANNNNTTC | 1 cut(s) | 721 |
| XspI | CTAG | 3 cut(s) | 363, 593, 818 |