transposition, RNA-mediated
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 1 cut(s) | 567 |
| AccB7I | CCANNNNNTGG | 2 cut(s) | 847, 1127 |
| AccII | CGCG | 3 cut(s) | 438, 761, 1201 |
| AciI | CCGC | 6 cut(s) | 151, 314, 690, 953, 1199, 1265 |
| AclWI | GGATC | 6 cut(s) | 274, 343, 453, 518, 987, 1188 |
| AcoI | YGGCCR | 2 cut(s) | 427, 885 |
| AcsI | RAATTY | 2 cut(s) | 557, 799 |
| AcyI | GRCGYC | 2 cut(s) | 568, 1269 |
| AfaI | GTAC | 4 cut(s) | 90, 707, 951, 1157 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 696, 699, 739, 847, 1000, 1127, 1146 |
| AflII | CTTAAG | 1 cut(s) | 830 |
| AgsI | TTSAA | 6 cut(s) | 399, 463, 809, 902, 928, 1012 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 763 |
| AjnI | CCWGG | 2 cut(s) | 738, 916 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 1233, 1265 |
| AluBI | AGCT | 4 cut(s) | 15, 275, 814, 908 |
| AluI | AGCT | 4 cut(s) | 15, 275, 814, 908 |
| Alw21I | GWGCWC | 1 cut(s) | 17 |
| Alw26I | GTCTC | 3 cut(s) | 408, 737, 915 |
| AlwI | GGATC | 6 cut(s) | 274, 343, 453, 518, 987, 1188 |
| AoxI | GGCC | 3 cut(s) | 57, 427, 885 |
| ApeKI | GCWGC | 3 cut(s) | 374, 377, 1256 |
| ApoI | RAATTY | 2 cut(s) | 557, 799 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1221 |
| AspLEI | GCGC | 4 cut(s) | 255, 440, 570, 1203 |
| AspS9I | GGNCC | 1 cut(s) | 386 |
| AsuC2I | CCSGG | 1 cut(s) | 1141 |
| AsuHPI | GGTGA | 3 cut(s) | 523, 1034, 1039 |
| AvaII | GGWCC | 1 cut(s) | 386 |
| BaeGI | GKGCMC | 2 cut(s) | 1096, 1176 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 933, 966 |
| BalI | TGGCCA | 2 cut(s) | 429, 887 |
| BanI | GGYRCC | 1 cut(s) | 567 |
| BanII | GRGCYC | 1 cut(s) | 17 |
| BauI | CACGAG | 1 cut(s) | 309 |
| Bbv12I | GWGCWC | 1 cut(s) | 17 |
| BbvI | GCAGC | 3 cut(s) | 386, 389, 1243 |
| BccI | CCATC | 7 cut(s) | 150, 218, 645, 728, 1163, 1167, 1223 |
| BceAI | ACGGC | 1 cut(s) | 677 |
| BciT130I | CCWGG | 2 cut(s) | 740, 918 |
| BcnI | CCSGG | 1 cut(s) | 1141 |
| BcoDI | GTCTC | 3 cut(s) | 408, 737, 915 |
| BfaI | CTAG | 4 cut(s) | 959, 983, 1083, 1274 |
| BfoI | RGCGCY | 1 cut(s) | 571 |
| BfrI | CTTAAG | 1 cut(s) | 830 |
| BisI | GCNGC | 4 cut(s) | 152, 375, 378, 1257 |
| BlsI | GCNGC | 4 cut(s) | 153, 376, 379, 1258 |
| Bme1390I | CCNGG | 3 cut(s) | 740, 918, 1141 |
| Bme18I | GGWCC | 1 cut(s) | 386 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 763 |
| BmgT120I | GGNCC | 1 cut(s) | 386 |
| BmiI | GGNNCC | 4 cut(s) | 229, 387, 388, 569 |
| BmrFI | CCNGG | 3 cut(s) | 740, 918, 1141 |
| BmsI | GCATC | 2 cut(s) | 706, 1015 |
| Bpu10I | CCTNAGC | 2 cut(s) | 285, 572 |
| BpuEI | CTTGAG | 1 cut(s) | 31 |
| BpuMI | CCSGG | 1 cut(s) | 1141 |
| BsaBI | GATNNNNATC | 1 cut(s) | 160 |
| BsaHI | GRCGYC | 2 cut(s) | 568, 1269 |
| BsaJI | CCNNGG | 3 cut(s) | 381, 916, 1121 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 734, 764 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 696, 699, 739, 847, 1000, 1127, 1146 |
| Bse118I | RCCGGY | 1 cut(s) | 564 |
| Bse1I | ACTGG | 2 cut(s) | 841, 1132 |
| Bse3DI | GCAATG | 1 cut(s) | 1210 |
| Bse8I | GATNNNNATC | 1 cut(s) | 160 |
| BseBI | CCWGG | 2 cut(s) | 740, 918 |
| BseDI | CCNNGG | 3 cut(s) | 381, 916, 1121 |
| BseGI | GGATG | 4 cut(s) | 174, 205, 258, 720 |
| BseJI | GATNNNNATC | 1 cut(s) | 160 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 696, 699, 739, 847, 1000, 1127, 1146 |
| BseMI | GCAATG | 1 cut(s) | 1210 |
| BseMII | CTCAG | 2 cut(s) | 88, 276 |
| BseNI | ACTGG | 2 cut(s) | 841, 1132 |
| BseRI | GAGGAG | 3 cut(s) | 606, 879, 1020 |
| BseSI | GKGCMC | 2 cut(s) | 1096, 1176 |
| BseXI | GCAGC | 3 cut(s) | 386, 389, 1243 |
| BseYI | CCCAGC | 1 cut(s) | 1261 |
| Bsh1236I | CGCG | 3 cut(s) | 438, 761, 1201 |
| Bsh1285I | CGRYCG | 1 cut(s) | 565 |
| BshFI | GGCC | 3 cut(s) | 59, 429, 887 |
| BshNI | GGYRCC | 1 cut(s) | 567 |
| BsiEI | CGRYCG | 1 cut(s) | 565 |
| BsiHKAI | GWGCWC | 1 cut(s) | 17 |
| BsiSI | CCGG | 4 cut(s) | 565, 788, 1140, 1253 |
| BslFI | GGGAC | 1 cut(s) | 372 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 696, 699, 739, 847, 1000, 1127, 1146 |
| BsmAI | GTCTC | 3 cut(s) | 408, 737, 915 |
| BsmFI | GGGAC | 1 cut(s) | 372 |
| BsmI | GAATGC | 1 cut(s) | 1248 |
| BsnI | GGCC | 3 cut(s) | 59, 429, 887 |
| Bsp1286I | GDGCHC | 3 cut(s) | 17, 1096, 1176 |
| Bsp143I | GATC | 8 cut(s) | 48, 161, 279, 335, 445, 523, 979, 1193 |
| BspACI | CCGC | 6 cut(s) | 151, 314, 690, 953, 1199, 1265 |
| BspANI | GGCC | 3 cut(s) | 59, 429, 887 |
| BspCNI | CTCAG | 2 cut(s) | 87, 277 |
| BspFNI | CGCG | 3 cut(s) | 438, 761, 1201 |
| BspLI | GGNNCC | 4 cut(s) | 229, 387, 388, 569 |
| BspPI | GGATC | 6 cut(s) | 274, 343, 453, 518, 987, 1188 |
| BspQI | GCTCTTC | 1 cut(s) | 426 |
| BspT107I | GGYRCC | 1 cut(s) | 567 |
| BspTI | CTTAAG | 1 cut(s) | 830 |
| BsrDI | GCAATG | 1 cut(s) | 1210 |
| BsrFI | RCCGGY | 1 cut(s) | 564 |
| BsrI | ACTGG | 2 cut(s) | 841, 1132 |
| BssAI | RCCGGY | 1 cut(s) | 564 |
| BssECI | CCNNGG | 3 cut(s) | 381, 916, 1121 |
| BssMI | GATC | 8 cut(s) | 48, 161, 279, 335, 445, 523, 979, 1193 |
| BssNI | GRCGYC | 2 cut(s) | 568, 1269 |
| BssSI | CACGAG | 1 cut(s) | 309 |
| BssT1I | CCWWGG | 2 cut(s) | 381, 1121 |
| Bst2BI | CACGAG | 1 cut(s) | 309 |
| Bst2UI | CCWGG | 2 cut(s) | 740, 918 |
| Bst6I | CTCTTC | 2 cut(s) | 200, 426 |
| BstACI | GRCGYC | 2 cut(s) | 568, 1269 |
| BstAFI | CTTAAG | 1 cut(s) | 830 |
| BstC8I | GCNNGC | 3 cut(s) | 291, 486, 490 |
| BstDEI | CTNAG | 5 cut(s) | 74, 285, 326, 572, 1106 |
| BstEII | GGTNACC | 1 cut(s) | 1045 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 737 |
| BstF5I | GGATG | 4 cut(s) | 174, 205, 258, 720 |
| BstFNI | CGCG | 3 cut(s) | 438, 761, 1201 |
| BstH2I | RGCGCY | 1 cut(s) | 571 |
| BstHHI | GCGC | 4 cut(s) | 255, 440, 570, 1203 |
| BstKTI | GATC | 8 cut(s) | 51, 164, 282, 338, 448, 526, 982, 1196 |
| BstMAI | GTCTC | 3 cut(s) | 408, 737, 915 |
| BstMBI | GATC | 8 cut(s) | 48, 161, 279, 335, 445, 523, 979, 1193 |
| BstMCI | CGRYCG | 1 cut(s) | 565 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 151, 435, 893, 1262 |
| BstNI | CCWGG | 2 cut(s) | 740, 918 |
| BstNSI | RCATGY | 2 cut(s) | 89, 496 |
| BstPI | GGTNACC | 1 cut(s) | 1045 |
| BstSCI | CCNGG | 3 cut(s) | 738, 916, 1139 |
| BstSLI | GKGCMC | 2 cut(s) | 1096, 1176 |
| BstUI | CGCG | 3 cut(s) | 438, 761, 1201 |
| BstV1I | GCAGC | 3 cut(s) | 386, 389, 1243 |
| BstX2I | RGATCY | 1 cut(s) | 979 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 507 |
| BstYI | RGATCY | 1 cut(s) | 979 |
| BsuRI | GGCC | 3 cut(s) | 59, 429, 887 |
| BtgZI | GCGATG | 3 cut(s) | 169, 768, 776 |
| BtsCI | GGATG | 4 cut(s) | 174, 205, 258, 720 |
| Cac8I | GCNNGC | 3 cut(s) | 291, 486, 490 |
| CfoI | GCGC | 4 cut(s) | 255, 440, 570, 1203 |
| Cfr10I | RCCGGY | 1 cut(s) | 564 |
| Cfr13I | GGNCC | 1 cut(s) | 386 |
| CseI | GACGC | 4 cut(s) | 91, 767, 830, 1277 |
| Csp6I | GTAC | 4 cut(s) | 89, 706, 950, 1156 |
| CviAII | CATG | 9 cut(s) | 55, 86, 178, 425, 493, 768, 976, 995, 1283 |
| CviQI | GTAC | 4 cut(s) | 89, 706, 950, 1156 |
| DdeI | CTNAG | 5 cut(s) | 74, 285, 326, 572, 1106 |
| DinI | GGCGCC | 1 cut(s) | 569 |
| DpnI | GATC | 8 cut(s) | 50, 163, 281, 337, 447, 525, 981, 1195 |
| DpnII | GATC | 8 cut(s) | 48, 161, 279, 335, 445, 523, 979, 1193 |
| DriI | GACNNNNNGTC | 1 cut(s) | 763 |
| EaeI | YGGCCR | 2 cut(s) | 427, 885 |
| Eam1104I | CTCTTC | 2 cut(s) | 200, 426 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 763 |
| EarI | CTCTTC | 2 cut(s) | 200, 426 |
| EciI | GGCGGA | 1 cut(s) | 679 |
| Ecl136II | GAGCTC | 1 cut(s) | 15 |
| Eco130I | CCWWGG | 2 cut(s) | 381, 1121 |
| Eco24I | GRGCYC | 1 cut(s) | 17 |
| Eco47I | GGWCC | 1 cut(s) | 386 |
| Eco53kI | GAGCTC | 1 cut(s) | 15 |
| Eco91I | GGTNACC | 1 cut(s) | 1045 |
| EcoICRI | GAGCTC | 1 cut(s) | 15 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 737 |
| EcoO109I | RGGNCCY | 1 cut(s) | 386 |
| EcoO65I | GGTNACC | 1 cut(s) | 1045 |
| EcoRI | GAATTC | 1 cut(s) | 557 |
| EcoRII | CCWGG | 2 cut(s) | 738, 916 |
| EcoT14I | CCWWGG | 2 cut(s) | 381, 1121 |
| EcoT38I | GRGCYC | 1 cut(s) | 17 |
| EgeI | GGCGCC | 1 cut(s) | 569 |
| EheI | GGCGCC | 1 cut(s) | 569 |
| ErhI | CCWWGG | 2 cut(s) | 381, 1121 |
| FaeI | CATG | 9 cut(s) | 58, 89, 181, 428, 496, 771, 979, 998, 1286 |
| FaqI | GGGAC | 1 cut(s) | 372 |
| FatI | CATG | 9 cut(s) | 54, 85, 177, 424, 492, 767, 975, 994, 1282 |
| FauI | CCCGC | 1 cut(s) | 1206 |
| FauNDI | CATATG | 1 cut(s) | 1018 |
| Fnu4HI | GCNGC | 4 cut(s) | 152, 375, 378, 1257 |
| FokI | GGATG | 4 cut(s) | 181, 212, 245, 707 |
| FriOI | GRGCYC | 1 cut(s) | 17 |
| Fsp4HI | GCNGC | 4 cut(s) | 152, 375, 378, 1257 |
| FspBI | CTAG | 4 cut(s) | 959, 983, 1083, 1274 |
| GlaI | GCGC | 4 cut(s) | 254, 439, 569, 1202 |
| GluI | GCNGC | 4 cut(s) | 152, 375, 378, 1257 |
| GsaI | CCCAGC | 1 cut(s) | 1265 |
| HaeII | RGCGCY | 1 cut(s) | 571 |
| HaeIII | GGCC | 3 cut(s) | 59, 429, 887 |
| HapII | CCGG | 4 cut(s) | 565, 788, 1140, 1253 |
| HgaI | GACGC | 4 cut(s) | 91, 767, 830, 1277 |
| HhaI | GCGC | 4 cut(s) | 255, 440, 570, 1203 |
| Hin1I | GRCGYC | 2 cut(s) | 568, 1269 |
| Hin1II | CATG | 9 cut(s) | 58, 89, 181, 428, 496, 771, 979, 998, 1286 |
| Hin6I | GCGC | 4 cut(s) | 253, 438, 568, 1201 |
| HinP1I | GCGC | 4 cut(s) | 253, 438, 568, 1201 |
| HincII | GTYRAC | 3 cut(s) | 36, 115, 1060 |
| HindII | GTYRAC | 3 cut(s) | 36, 115, 1060 |
| HpaII | CCGG | 4 cut(s) | 565, 788, 1140, 1253 |
| HphI | GGTGA | 3 cut(s) | 523, 1034, 1039 |
| Hpy166II | GTNNAC | 5 cut(s) | 36, 115, 853, 1060, 1235 |
| Hpy188I | TCNGA | 4 cut(s) | 279, 349, 591, 863 |
| Hpy188III | TCNNGA | 5 cut(s) | 10, 508, 809, 1163, 1226 |
| Hpy8I | GTNNAC | 5 cut(s) | 36, 115, 853, 1060, 1235 |
| Hpy99I | CGWCG | 5 cut(s) | 23, 85, 124, 243, 716 |
| HpyAV | CCTTC | 7 cut(s) | 16, 70, 175, 179, 326, 548, 994 |
| HpyCH4IV | ACGT | 1 cut(s) | 233 |
| HpyCH4V | TGCA | 3 cut(s) | 374, 1248, 1282 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 151, 435, 893, 1262 |
| HpyF3I | CTNAG | 5 cut(s) | 74, 285, 326, 572, 1106 |
| HpySE526I | ACGT | 1 cut(s) | 233 |
| Hsp92I | GRCGYC | 2 cut(s) | 568, 1269 |
| Hsp92II | CATG | 9 cut(s) | 58, 89, 181, 428, 496, 771, 979, 998, 1286 |
| HspAI | GCGC | 4 cut(s) | 253, 438, 568, 1201 |
| KasI | GGCGCC | 1 cut(s) | 567 |
| KflI | GGGWCCC | 1 cut(s) | 386 |
| Kzo9I | GATC | 8 cut(s) | 48, 161, 279, 335, 445, 523, 979, 1193 |
| LguI | GCTCTTC | 1 cut(s) | 426 |
| LmnI | GCTCC | 3 cut(s) | 20, 316, 1033 |
| Lsp1109I | GCAGC | 3 cut(s) | 386, 389, 1243 |
| LweI | GCATC | 2 cut(s) | 706, 1015 |
| MaeI | CTAG | 4 cut(s) | 959, 983, 1083, 1274 |
| MaeII | ACGT | 1 cut(s) | 233 |
| MaeIII | GTNAC | 3 cut(s) | 650, 755, 1045 |
| MalI | GATC | 8 cut(s) | 50, 163, 281, 337, 447, 525, 981, 1195 |
| MboI | GATC | 8 cut(s) | 48, 161, 279, 335, 445, 523, 979, 1193 |
| MboII | GAAGA | 5 cut(s) | 217, 229, 413, 530, 1226 |
| MfeI | CAATTG | 1 cut(s) | 603 |
| MflI | RGATCY | 1 cut(s) | 979 |
| MhlI | GDGCHC | 3 cut(s) | 17, 1096, 1176 |
| MlsI | TGGCCA | 2 cut(s) | 429, 887 |
| MluCI | AATT | 4 cut(s) | 363, 557, 603, 799 |
| MluNI | TGGCCA | 2 cut(s) | 429, 887 |
| Mly113I | GGCGCC | 1 cut(s) | 568 |
| MlyI | GAGTC | 6 cut(s) | 32, 110, 114, 513, 848, 876 |
| Mox20I | TGGCCA | 2 cut(s) | 429, 887 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1221 |
| MscI | TGGCCA | 2 cut(s) | 429, 887 |
| MseI | TTAA | 4 cut(s) | 96, 629, 684, 831 |
| MslI | CAYNNNNRTG | 2 cut(s) | 645, 714 |
| Msp20I | TGGCCA | 2 cut(s) | 429, 887 |
| MspA1I | CMGCKG | 1 cut(s) | 1265 |
| MspCI | CTTAAG | 1 cut(s) | 830 |
| MspI | CCGG | 4 cut(s) | 565, 788, 1140, 1253 |
| MspR9I | CCNGG | 3 cut(s) | 740, 918, 1141 |
| MunI | CAATTG | 1 cut(s) | 603 |
| Mva1269I | GAATGC | 1 cut(s) | 1248 |
| MvaI | CCWGG | 2 cut(s) | 740, 918 |
| MvnI | CGCG | 3 cut(s) | 438, 761, 1201 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 151, 435, 893, 1262 |
| NarI | GGCGCC | 1 cut(s) | 568 |
| NciI | CCSGG | 1 cut(s) | 1141 |
| NdeI | CATATG | 1 cut(s) | 1018 |
| NdeII | GATC | 8 cut(s) | 48, 161, 279, 335, 445, 523, 979, 1193 |
| NlaIII | CATG | 9 cut(s) | 58, 89, 181, 428, 496, 771, 979, 998, 1286 |
| NlaIV | GGNNCC | 4 cut(s) | 229, 387, 388, 569 |
| NmuCI | GTSAC | 3 cut(s) | 650, 755, 1045 |
| NspI | RCATGY | 2 cut(s) | 89, 496 |
| PciSI | GCTCTTC | 1 cut(s) | 426 |
| PctI | GAATGC | 1 cut(s) | 1248 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1221 |
| PfeI | GAWTC | 7 cut(s) | 356, 592, 610, 779, 791, 837, 1087 |
| PflFI | GACNNNGTC | 2 cut(s) | 748, 971 |
| PflMI | CCANNNNNTGG | 2 cut(s) | 847, 1127 |
| PfoI | TCCNGGA | 1 cut(s) | 738 |
| PkrI | GCNGC | 4 cut(s) | 153, 376, 379, 1258 |
| PleI | GAGTC | 6 cut(s) | 31, 110, 113, 512, 848, 875 |
| PluTI | GGCGCC | 1 cut(s) | 571 |
| PpsI | GAGTC | 6 cut(s) | 31, 110, 113, 512, 848, 875 |
| PpuMI | RGGWCCY | 1 cut(s) | 386 |
| Psp124BI | GAGCTC | 1 cut(s) | 17 |
| Psp5II | RGGWCCY | 1 cut(s) | 386 |
| Psp6I | CCWGG | 2 cut(s) | 738, 916 |
| PspEI | GGTNACC | 1 cut(s) | 1045 |
| PspFI | CCCAGC | 1 cut(s) | 1261 |
| PspGI | CCWGG | 2 cut(s) | 738, 916 |
| PspN4I | GGNNCC | 4 cut(s) | 229, 387, 388, 569 |
| PspPI | GGNCC | 1 cut(s) | 386 |
| PspPPI | RGGWCCY | 1 cut(s) | 386 |
| PsuI | RGATCY | 1 cut(s) | 979 |
| PsyI | GACNNNGTC | 2 cut(s) | 748, 971 |
| RsaI | GTAC | 4 cut(s) | 90, 707, 951, 1157 |
| RsaNI | GTAC | 4 cut(s) | 89, 706, 950, 1156 |
| RseI | CAYNNNNRTG | 2 cut(s) | 645, 714 |
| SacI | GAGCTC | 1 cut(s) | 17 |
| SapI | GCTCTTC | 1 cut(s) | 426 |
| SaqAI | TTAA | 4 cut(s) | 96, 629, 684, 831 |
| SatI | GCNGC | 4 cut(s) | 152, 375, 378, 1257 |
| Sau3AI | GATC | 8 cut(s) | 48, 161, 279, 335, 445, 523, 979, 1193 |
| Sau96I | GGNCC | 1 cut(s) | 386 |
| SchI | GAGTC | 6 cut(s) | 32, 110, 114, 513, 848, 876 |
| ScrFI | CCNGG | 3 cut(s) | 740, 918, 1141 |
| SduI | GDGCHC | 3 cut(s) | 17, 1096, 1176 |
| SfaNI | GCATC | 2 cut(s) | 706, 1015 |
| SfoI | GGCGCC | 1 cut(s) | 569 |
| SinI | GGWCC | 1 cut(s) | 386 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 645, 714 |
| SmlI | CTYRAG | 2 cut(s) | 10, 830 |
| SmoI | CTYRAG | 2 cut(s) | 10, 830 |
| Sse9I | AATT | 4 cut(s) | 363, 557, 603, 799 |
| SsiI | CCGC | 6 cut(s) | 151, 314, 690, 953, 1199, 1265 |
| SspDI | GGCGCC | 1 cut(s) | 567 |
| SspI | AATATT | 1 cut(s) | 932 |
| SspMI | CTAG | 4 cut(s) | 959, 983, 1083, 1274 |
| SstI | GAGCTC | 1 cut(s) | 17 |
| StyD4I | CCNGG | 3 cut(s) | 738, 916, 1139 |
| StyI | CCWWGG | 2 cut(s) | 381, 1121 |
| TaiI | ACGT | 1 cut(s) | 236 |
| TaqI | TCGA | 5 cut(s) | 21, 51, 561, 1039, 1225 |
| TasI | AATT | 4 cut(s) | 363, 557, 603, 799 |
| TauI | GCSGC | 1 cut(s) | 154 |
| TfiI | GAWTC | 7 cut(s) | 356, 592, 610, 779, 791, 837, 1087 |
| Tru1I | TTAA | 4 cut(s) | 96, 629, 684, 831 |
| Tru9I | TTAA | 4 cut(s) | 96, 629, 684, 831 |
| TseFI | GTSAC | 3 cut(s) | 650, 755, 1045 |
| TseI | GCWGC | 3 cut(s) | 374, 377, 1256 |
| Tsp45I | GTSAC | 3 cut(s) | 650, 755, 1045 |
| TspDTI | ATGAA | 7 cut(s) | 52, 185, 194, 413, 792, 792, 1092 |
| TspGWI | ACGGA | 1 cut(s) | 7 |
| Tth111I | GACNNNGTC | 2 cut(s) | 748, 971 |
| Van91I | CCANNNNNTGG | 2 cut(s) | 847, 1127 |
| Vha464I | CTTAAG | 1 cut(s) | 830 |
| VpaK11BI | GGWCC | 1 cut(s) | 386 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 737 |
| XapI | RAATTY | 2 cut(s) | 557, 799 |
| XceI | RCATGY | 2 cut(s) | 89, 496 |
| XcmI | CCANNNNNNNNNTGG | 2 cut(s) | 644, 1166 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1221 |
| XspI | CTAG | 4 cut(s) | 959, 983, 1083, 1274 |