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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 70 |
| Acc36I | ACCTGC | 1 cut(s) | 70 |
| Acc65I | GGTACC | 2 cut(s) | 202, 229 |
| AccB1I | GGYRCC | 2 cut(s) | 202, 229 |
| AccBSI | CCGCTC | 1 cut(s) | 327 |
| AccI | GTMKAC | 1 cut(s) | 181 |
| AccII | CGCG | 1 cut(s) | 163 |
| AciI | CCGC | 8 cut(s) | 20, 163, 226, 233, 280, 302, 314, 325 |
| AcoI | YGGCCR | 1 cut(s) | 84 |
| AcsI | RAATTY | 1 cut(s) | 101 |
| AfaI | GTAC | 3 cut(s) | 204, 210, 231 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 31, 79, 191, 289, 372 |
| AgeI | ACCGGT | 1 cut(s) | 191 |
| AjnI | CCWGG | 1 cut(s) | 395 |
| AluBI | AGCT | 2 cut(s) | 10, 378 |
| AluI | AGCT | 2 cut(s) | 10, 378 |
| Alw21I | GWGCWC | 1 cut(s) | 170 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 378 |
| AoxI | GGCC | 3 cut(s) | 69, 84, 403 |
| ApaI | GGGCCC | 1 cut(s) | 407 |
| ApeKI | GCWGC | 1 cut(s) | 124 |
| ApoI | RAATTY | 1 cut(s) | 101 |
| AsiGI | ACCGGT | 1 cut(s) | 191 |
| Asp718I | GGTACC | 2 cut(s) | 202, 229 |
| AspLEI | GCGC | 3 cut(s) | 54, 124, 148 |
| AspS9I | GGNCC | 5 cut(s) | 22, 70, 262, 403, 404 |
| AvaII | GGWCC | 2 cut(s) | 22, 262 |
| BaeGI | GKGCMC | 1 cut(s) | 407 |
| BalI | TGGCCA | 1 cut(s) | 86 |
| BanI | GGYRCC | 2 cut(s) | 202, 229 |
| BanII | GRGCYC | 1 cut(s) | 407 |
| Bbv12I | GWGCWC | 1 cut(s) | 170 |
| BbvI | GCAGC | 1 cut(s) | 111 |
| BccI | CCATC | 1 cut(s) | 283 |
| BciT130I | CCWGG | 1 cut(s) | 397 |
| BfmI | CTRYAG | 1 cut(s) | 125 |
| BfoI | RGCGCY | 1 cut(s) | 125 |
| BfuAI | ACCTGC | 1 cut(s) | 70 |
| BisI | GCNGC | 1 cut(s) | 125 |
| BlsI | GCNGC | 1 cut(s) | 126 |
| Bme1390I | CCNGG | 1 cut(s) | 397 |
| Bme18I | GGWCC | 2 cut(s) | 22, 262 |
| BmgT120I | GGNCC | 5 cut(s) | 22, 70, 262, 403, 404 |
| BmiI | GGNNCC | 4 cut(s) | 204, 231, 264, 405 |
| BmrFI | CCNGG | 1 cut(s) | 397 |
| BmsI | GCATC | 1 cut(s) | 213 |
| BpmI | CTGGAG | 1 cut(s) | 379 |
| BsaWI | WCCGGW | 1 cut(s) | 191 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 31, 79, 191, 289, 372 |
| Bse118I | RCCGGY | 1 cut(s) | 191 |
| Bse1I | ACTGG | 2 cut(s) | 206, 414 |
| BseBI | CCWGG | 1 cut(s) | 397 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 31, 79, 191, 289, 372 |
| BseMII | CTCAG | 1 cut(s) | 338 |
| BseNI | ACTGG | 2 cut(s) | 206, 414 |
| BseSI | GKGCMC | 1 cut(s) | 407 |
| BseXI | GCAGC | 1 cut(s) | 111 |
| BseYI | CCCAGC | 1 cut(s) | 142 |
| Bsh1236I | CGCG | 1 cut(s) | 163 |
| BshFI | GGCC | 3 cut(s) | 71, 86, 405 |
| BshNI | GGYRCC | 2 cut(s) | 202, 229 |
| BshTI | ACCGGT | 1 cut(s) | 191 |
| BsiHKAI | GWGCWC | 1 cut(s) | 170 |
| BsiSI | CCGG | 1 cut(s) | 192 |
| BslFI | GGGAC | 1 cut(s) | 349 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 31, 79, 191, 289, 372 |
| BsmFI | GGGAC | 1 cut(s) | 349 |
| BsnI | GGCC | 3 cut(s) | 71, 86, 405 |
| Bsp120I | GGGCCC | 1 cut(s) | 403 |
| Bsp1286I | GDGCHC | 2 cut(s) | 170, 407 |
| BspACI | CCGC | 8 cut(s) | 20, 163, 226, 233, 280, 302, 314, 325 |
| BspANI | GGCC | 3 cut(s) | 71, 86, 405 |
| BspCNI | CTCAG | 1 cut(s) | 339 |
| BspFNI | CGCG | 1 cut(s) | 163 |
| BspLI | GGNNCC | 4 cut(s) | 204, 231, 264, 405 |
| BspMAI | CTGCAG | 1 cut(s) | 129 |
| BspMI | ACCTGC | 1 cut(s) | 70 |
| BspT107I | GGYRCC | 2 cut(s) | 202, 229 |
| BsrBI | CCGCTC | 1 cut(s) | 327 |
| BsrFI | RCCGGY | 1 cut(s) | 191 |
| BsrI | ACTGG | 2 cut(s) | 206, 414 |
| BssAI | RCCGGY | 1 cut(s) | 191 |
| Bst2UI | CCWGG | 1 cut(s) | 397 |
| BstC8I | GCNNGC | 1 cut(s) | 12 |
| BstDEI | CTNAG | 1 cut(s) | 347 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 29 |
| BstFNI | CGCG | 1 cut(s) | 163 |
| BstH2I | RGCGCY | 1 cut(s) | 125 |
| BstHHI | GCGC | 3 cut(s) | 54, 124, 148 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 143, 154, 174, 232, 384 |
| BstNI | CCWGG | 1 cut(s) | 397 |
| BstSCI | CCNGG | 1 cut(s) | 395 |
| BstSFI | CTRYAG | 1 cut(s) | 125 |
| BstSLI | GKGCMC | 1 cut(s) | 407 |
| BstUI | CGCG | 1 cut(s) | 163 |
| BstV1I | GCAGC | 1 cut(s) | 111 |
| BsuRI | GGCC | 3 cut(s) | 71, 86, 405 |
| BtsIMutI | CAGTG | 1 cut(s) | 407 |
| BveI | ACCTGC | 1 cut(s) | 70 |
| Cac8I | GCNNGC | 1 cut(s) | 12 |
| CaiI | CAGNNNCTG | 1 cut(s) | 378 |
| CfoI | GCGC | 3 cut(s) | 54, 124, 148 |
| Cfr10I | RCCGGY | 1 cut(s) | 191 |
| Cfr13I | GGNCC | 5 cut(s) | 22, 70, 262, 403, 404 |
| CseI | GACGC | 1 cut(s) | 183 |
| Csp6I | GTAC | 3 cut(s) | 203, 209, 230 |
| CspAI | ACCGGT | 1 cut(s) | 191 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 175, 210 |
| CviAII | CATG | 1 cut(s) | 296 |
| CviJI | RGCY | 5 cut(s) | 10, 71, 86, 378, 405 |
| CviKI_1 | RGCY | 5 cut(s) | 10, 71, 86, 378, 405 |
| CviQI | GTAC | 3 cut(s) | 203, 209, 230 |
| DdeI | CTNAG | 1 cut(s) | 347 |
| EaeI | YGGCCR | 1 cut(s) | 84 |
| EciI | GGCGGA | 2 cut(s) | 35, 317 |
| Eco24I | GRGCYC | 1 cut(s) | 407 |
| Eco47I | GGWCC | 2 cut(s) | 22, 262 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 29 |
| EcoO109I | RGGNCCY | 1 cut(s) | 403 |
| EcoRII | CCWGG | 1 cut(s) | 395 |
| EcoT38I | GRGCYC | 1 cut(s) | 407 |
| FaeI | CATG | 1 cut(s) | 299 |
| FaiI | YATR | 3 cut(s) | 6, 218, 297 |
| FaqI | GGGAC | 1 cut(s) | 349 |
| FatI | CATG | 1 cut(s) | 295 |
| FauI | CCCGC | 2 cut(s) | 219, 287 |
| FblI | GTMKAC | 1 cut(s) | 181 |
| Fnu4HI | GCNGC | 1 cut(s) | 125 |
| FriOI | GRGCYC | 1 cut(s) | 407 |
| Fsp4HI | GCNGC | 1 cut(s) | 125 |
| GlaI | GCGC | 3 cut(s) | 53, 123, 147 |
| GluI | GCNGC | 1 cut(s) | 125 |
| GsaI | CCCAGC | 1 cut(s) | 146 |
| GsuI | CTGGAG | 1 cut(s) | 379 |
| HaeII | RGCGCY | 1 cut(s) | 125 |
| HaeIII | GGCC | 3 cut(s) | 71, 86, 405 |
| HapII | CCGG | 1 cut(s) | 192 |
| HgaI | GACGC | 1 cut(s) | 183 |
| HhaI | GCGC | 3 cut(s) | 54, 124, 148 |
| Hin1II | CATG | 1 cut(s) | 299 |
| Hin6I | GCGC | 3 cut(s) | 52, 122, 146 |
| HinP1I | GCGC | 3 cut(s) | 52, 122, 146 |
| HincII | GTYRAC | 1 cut(s) | 182 |
| HindII | GTYRAC | 1 cut(s) | 182 |
| HinfI | GANTC | 1 cut(s) | 305 |
| HpaII | CCGG | 1 cut(s) | 192 |
| Hpy166II | GTNNAC | 1 cut(s) | 182 |
| Hpy8I | GTNNAC | 1 cut(s) | 182 |
| Hpy99I | CGWCG | 1 cut(s) | 110 |
| HpyAV | CCTTC | 1 cut(s) | 55 |
| HpyCH4V | TGCA | 1 cut(s) | 127 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 143, 154, 174, 232, 384 |
| HpyF3I | CTNAG | 1 cut(s) | 347 |
| Hsp92II | CATG | 1 cut(s) | 299 |
| HspAI | GCGC | 3 cut(s) | 52, 122, 146 |
| KpnI | GGTACC | 2 cut(s) | 206, 233 |
| LmnI | GCTCC | 3 cut(s) | 154, 165, 375 |
| Lsp1109I | GCAGC | 1 cut(s) | 111 |
| LweI | GCATC | 1 cut(s) | 213 |
| MaeIII | GTNAC | 1 cut(s) | 306 |
| MbiI | CCGCTC | 1 cut(s) | 327 |
| MhlI | GDGCHC | 2 cut(s) | 170, 407 |
| MlsI | TGGCCA | 1 cut(s) | 86 |
| MluCI | AATT | 1 cut(s) | 101 |
| MluNI | TGGCCA | 1 cut(s) | 86 |
| MlyI | GAGTC | 1 cut(s) | 314 |
| MnlI | CCTC | 6 cut(s) | 35, 61, 144, 279, 292, 342 |
| Mox20I | TGGCCA | 1 cut(s) | 86 |
| MscI | TGGCCA | 1 cut(s) | 86 |
| MslI | CAYNNNNRTG | 2 cut(s) | 92, 221 |
| Msp20I | TGGCCA | 1 cut(s) | 86 |
| MspA1I | CMGCKG | 1 cut(s) | 235 |
| MspI | CCGG | 1 cut(s) | 192 |
| MspR9I | CCNGG | 1 cut(s) | 397 |
| MvaI | CCWGG | 1 cut(s) | 397 |
| MvnI | CGCG | 1 cut(s) | 163 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 143, 154, 174, 232, 384 |
| NlaIII | CATG | 1 cut(s) | 299 |
| NlaIV | GGNNCC | 4 cut(s) | 204, 231, 264, 405 |
| NmuCI | GTSAC | 1 cut(s) | 306 |
| PaqCI | CACCTGC | 1 cut(s) | 70 |
| PflFI | GACNNNGTC | 1 cut(s) | 177 |
| PinAI | ACCGGT | 1 cut(s) | 191 |
| PkrI | GCNGC | 1 cut(s) | 126 |
| PleI | GAGTC | 1 cut(s) | 313 |
| PpsI | GAGTC | 1 cut(s) | 313 |
| Psp6I | CCWGG | 1 cut(s) | 395 |
| PspFI | CCCAGC | 1 cut(s) | 142 |
| PspGI | CCWGG | 1 cut(s) | 395 |
| PspN4I | GGNNCC | 4 cut(s) | 204, 231, 264, 405 |
| PspOMI | GGGCCC | 1 cut(s) | 403 |
| PspPI | GGNCC | 5 cut(s) | 22, 70, 262, 403, 404 |
| PstI | CTGCAG | 1 cut(s) | 129 |
| PstNI | CAGNNNCTG | 1 cut(s) | 378 |
| PsyI | GACNNNGTC | 1 cut(s) | 177 |
| RsaI | GTAC | 3 cut(s) | 204, 210, 231 |
| RsaNI | GTAC | 3 cut(s) | 203, 209, 230 |
| RseI | CAYNNNNRTG | 2 cut(s) | 92, 221 |
| SalI | GTCGAC | 1 cut(s) | 180 |
| SatI | GCNGC | 1 cut(s) | 125 |
| Sau96I | GGNCC | 5 cut(s) | 22, 70, 262, 403, 404 |
| SchI | GAGTC | 1 cut(s) | 314 |
| ScrFI | CCNGG | 1 cut(s) | 397 |
| SduI | GDGCHC | 2 cut(s) | 170, 407 |
| SetI | ASST | 7 cut(s) | 12, 27, 66, 84, 271, 363, 380 |
| SfaNI | GCATC | 1 cut(s) | 213 |
| SfcI | CTRYAG | 1 cut(s) | 125 |
| SinI | GGWCC | 2 cut(s) | 22, 262 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 92, 221 |
| Sse9I | AATT | 1 cut(s) | 101 |
| SsiI | CCGC | 8 cut(s) | 20, 163, 226, 233, 280, 302, 314, 325 |
| StyD4I | CCNGG | 1 cut(s) | 395 |
| TaqI | TCGA | 3 cut(s) | 105, 181, 329 |
| TasI | AATT | 1 cut(s) | 101 |
| TscAI | CASTG | 1 cut(s) | 414 |
| TseFI | GTSAC | 1 cut(s) | 306 |
| TseI | GCWGC | 1 cut(s) | 124 |
| Tsp45I | GTSAC | 1 cut(s) | 306 |
| TspRI | CASTG | 1 cut(s) | 414 |
| Tth111I | GACNNNGTC | 1 cut(s) | 177 |
| VpaK11BI | GGWCC | 2 cut(s) | 22, 262 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 29 |
| XapI | RAATTY | 1 cut(s) | 101 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 375 |
| XmiI | GTMKAC | 1 cut(s) | 181 |