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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AciI | CCGC | 5 cut(s) | 15, 38, 74, 89, 135 |
| AclWI | GGATC | 3 cut(s) | 48, 135, 168 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 154 |
| AjnI | CCWGG | 1 cut(s) | 118 |
| Alw26I | GTCTC | 1 cut(s) | 116 |
| AlwI | GGATC | 3 cut(s) | 48, 135, 168 |
| AoxI | GGCC | 2 cut(s) | 121, 213 |
| ApeKI | GCWGC | 1 cut(s) | 105 |
| AspS9I | GGNCC | 1 cut(s) | 214 |
| AsuC2I | CCSGG | 1 cut(s) | 125 |
| BbvI | GCAGC | 1 cut(s) | 117 |
| BccI | CCATC | 1 cut(s) | 66 |
| BciT130I | CCWGG | 1 cut(s) | 120 |
| BcnI | CCSGG | 1 cut(s) | 125 |
| BcoDI | GTCTC | 1 cut(s) | 116 |
| BfaI | CTAG | 1 cut(s) | 101 |
| BfmI | CTRYAG | 1 cut(s) | 84 |
| BisI | GCNGC | 3 cut(s) | 75, 106, 135 |
| BlsI | GCNGC | 3 cut(s) | 76, 107, 136 |
| Bme1390I | CCNGG | 2 cut(s) | 120, 125 |
| BmgT120I | GGNCC | 1 cut(s) | 214 |
| BmrFI | CCNGG | 2 cut(s) | 120, 125 |
| BpuMI | CCSGG | 1 cut(s) | 125 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 154 |
| BseBI | CCWGG | 1 cut(s) | 120 |
| BseGI | GGATG | 1 cut(s) | 58 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 154 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseXI | GCAGC | 1 cut(s) | 117 |
| BshFI | GGCC | 2 cut(s) | 123, 215 |
| BsiSI | CCGG | 1 cut(s) | 124 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 154 |
| BsmAI | GTCTC | 1 cut(s) | 116 |
| BsnI | GGCC | 2 cut(s) | 123, 215 |
| Bsp143I | GATC | 3 cut(s) | 40, 127, 160 |
| BspACI | CCGC | 5 cut(s) | 15, 38, 74, 89, 135 |
| BspANI | GGCC | 2 cut(s) | 123, 215 |
| BspPI | GGATC | 3 cut(s) | 48, 135, 168 |
| BssMI | GATC | 3 cut(s) | 40, 127, 160 |
| Bst2UI | CCWGG | 1 cut(s) | 120 |
| Bst4CI | ACNGT | 1 cut(s) | 11 |
| BstC8I | GCNNGC | 1 cut(s) | 36 |
| BstF5I | GGATG | 1 cut(s) | 58 |
| BstKTI | GATC | 3 cut(s) | 43, 130, 163 |
| BstMAI | GTCTC | 1 cut(s) | 116 |
| BstMBI | GATC | 3 cut(s) | 40, 127, 160 |
| BstNI | CCWGG | 1 cut(s) | 120 |
| BstSCI | CCNGG | 2 cut(s) | 118, 123 |
| BstSFI | CTRYAG | 1 cut(s) | 84 |
| BstV1I | GCAGC | 1 cut(s) | 117 |
| BstX2I | RGATCY | 2 cut(s) | 127, 160 |
| BstYI | RGATCY | 2 cut(s) | 127, 160 |
| BsuRI | GGCC | 2 cut(s) | 123, 215 |
| BtsCI | GGATG | 1 cut(s) | 58 |
| Cac8I | GCNNGC | 1 cut(s) | 36 |
| Cfr13I | GGNCC | 1 cut(s) | 214 |
| CviAII | CATG | 1 cut(s) | 185 |
| CviJI | RGCY | 3 cut(s) | 77, 123, 215 |
| CviKI_1 | RGCY | 3 cut(s) | 77, 123, 215 |
| DpnI | GATC | 3 cut(s) | 42, 129, 162 |
| DpnII | GATC | 3 cut(s) | 40, 127, 160 |
| EcoRII | CCWGG | 1 cut(s) | 118 |
| FaeI | CATG | 1 cut(s) | 188 |
| FaiI | YATR | 4 cut(s) | 86, 140, 144, 186 |
| FatI | CATG | 1 cut(s) | 184 |
| FauI | CCCGC | 1 cut(s) | 8 |
| Fnu4HI | GCNGC | 3 cut(s) | 75, 106, 135 |
| FokI | GGATG | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 3 cut(s) | 75, 106, 135 |
| FspBI | CTAG | 1 cut(s) | 101 |
| GluI | GCNGC | 3 cut(s) | 75, 106, 135 |
| HaeIII | GGCC | 2 cut(s) | 123, 215 |
| HapII | CCGG | 1 cut(s) | 124 |
| Hin1II | CATG | 1 cut(s) | 188 |
| HpaII | CCGG | 1 cut(s) | 124 |
| HpyAV | CCTTC | 1 cut(s) | 199 |
| HpyCH4III | ACNGT | 1 cut(s) | 11 |
| Hsp92II | CATG | 1 cut(s) | 188 |
| Kzo9I | GATC | 3 cut(s) | 40, 127, 160 |
| LmnI | GCTCC | 1 cut(s) | 31 |
| LpnPI | CCDG | 6 cut(s) | 84, 105, 132, 137, 154, 161 |
| Lsp1109I | GCAGC | 1 cut(s) | 117 |
| MaeI | CTAG | 1 cut(s) | 101 |
| MalI | GATC | 3 cut(s) | 42, 129, 162 |
| MboI | GATC | 3 cut(s) | 40, 127, 160 |
| MflI | RGATCY | 2 cut(s) | 127, 160 |
| MluCI | AATT | 1 cut(s) | 44 |
| MnlI | CCTC | 3 cut(s) | 22, 85, 205 |
| MspI | CCGG | 1 cut(s) | 124 |
| MspR9I | CCNGG | 2 cut(s) | 120, 125 |
| MvaI | CCWGG | 1 cut(s) | 120 |
| NciI | CCSGG | 1 cut(s) | 125 |
| NdeII | GATC | 3 cut(s) | 40, 127, 160 |
| NlaIII | CATG | 1 cut(s) | 188 |
| PkrI | GCNGC | 3 cut(s) | 76, 107, 136 |
| Psp6I | CCWGG | 1 cut(s) | 118 |
| PspGI | CCWGG | 1 cut(s) | 118 |
| PspPI | GGNCC | 1 cut(s) | 214 |
| PsuI | RGATCY | 2 cut(s) | 127, 160 |
| SatI | GCNGC | 3 cut(s) | 75, 106, 135 |
| Sau3AI | GATC | 3 cut(s) | 40, 127, 160 |
| Sau96I | GGNCC | 1 cut(s) | 214 |
| ScrFI | CCNGG | 2 cut(s) | 120, 125 |
| SetI | ASST | 2 cut(s) | 150, 210 |
| SfcI | CTRYAG | 1 cut(s) | 84 |
| Sse9I | AATT | 1 cut(s) | 44 |
| SsiI | CCGC | 5 cut(s) | 15, 38, 74, 89, 135 |
| SspMI | CTAG | 1 cut(s) | 101 |
| StyD4I | CCNGG | 2 cut(s) | 118, 123 |
| TaaI | ACNGT | 1 cut(s) | 11 |
| TaqI | TCGA | 1 cut(s) | 210 |
| TasI | AATT | 1 cut(s) | 44 |
| TauI | GCSGC | 2 cut(s) | 77, 137 |
| TseI | GCWGC | 1 cut(s) | 105 |
| TspDTI | ATGAA | 1 cut(s) | 201 |
| XspI | CTAG | 1 cut(s) | 101 |