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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 247 |
| Acc36I | ACCTGC | 1 cut(s) | 247 |
| AccII | CGCG | 2 cut(s) | 90, 219 |
| AciI | CCGC | 8 cut(s) | 9, 15, 48, 74, 88, 149, 305, 312 |
| AclWI | GGATC | 1 cut(s) | 201 |
| AcoI | YGGCCR | 2 cut(s) | 108, 306 |
| AcuI | CTGAAG | 1 cut(s) | 264 |
| AcyI | GRCGYC | 1 cut(s) | 81 |
| AfaI | GTAC | 1 cut(s) | 176 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 77, 256 |
| AflIII | ACRYGT | 1 cut(s) | 275 |
| AluBI | AGCT | 1 cut(s) | 181 |
| AluI | AGCT | 1 cut(s) | 181 |
| AlwI | GGATC | 1 cut(s) | 201 |
| AoxI | GGCC | 3 cut(s) | 108, 154, 306 |
| ApeKI | GCWGC | 3 cut(s) | 12, 138, 263 |
| AspLEI | GCGC | 2 cut(s) | 92, 231 |
| AspS9I | GGNCC | 1 cut(s) | 154 |
| AsuC2I | CCSGG | 3 cut(s) | 107, 117, 153 |
| AsuHPI | GGTGA | 1 cut(s) | 134 |
| BbsI | GAAGAC | 1 cut(s) | 302 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 3 cut(s) | 24, 150, 250 |
| BcnI | CCSGG | 3 cut(s) | 107, 117, 153 |
| BfaI | CTAG | 2 cut(s) | 6, 171 |
| BfuAI | ACCTGC | 1 cut(s) | 247 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BglII | AGATCT | 1 cut(s) | 160 |
| BisI | GCNGC | 5 cut(s) | 10, 13, 139, 264, 306 |
| BlsI | GCNGC | 5 cut(s) | 11, 14, 140, 265, 307 |
| Bme1390I | CCNGG | 3 cut(s) | 107, 117, 153 |
| BmgT120I | GGNCC | 1 cut(s) | 154 |
| BmrFI | CCNGG | 3 cut(s) | 107, 117, 153 |
| BpiI | GAAGAC | 1 cut(s) | 302 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 3 cut(s) | 107, 117, 153 |
| BsaHI | GRCGYC | 1 cut(s) | 81 |
| BsaJI | CCNNGG | 2 cut(s) | 105, 115 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 77, 256 |
| BseDI | CCNNGG | 2 cut(s) | 105, 115 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 77, 256 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseXI | GCAGC | 3 cut(s) | 24, 150, 250 |
| BseYI | CCCAGC | 2 cut(s) | 70, 319 |
| Bsh1236I | CGCG | 2 cut(s) | 90, 219 |
| BshFI | GGCC | 3 cut(s) | 110, 156, 308 |
| BsiSI | CCGG | 3 cut(s) | 107, 117, 152 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 77, 256 |
| BsnI | GGCC | 3 cut(s) | 110, 156, 308 |
| Bsp143I | GATC | 2 cut(s) | 160, 193 |
| BspACI | CCGC | 8 cut(s) | 9, 15, 48, 74, 88, 149, 305, 312 |
| BspANI | GGCC | 3 cut(s) | 110, 156, 308 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspFNI | CGCG | 2 cut(s) | 90, 219 |
| BspHI | TCATGA | 1 cut(s) | 270 |
| BspMI | ACCTGC | 1 cut(s) | 247 |
| BspPI | GGATC | 1 cut(s) | 201 |
| BssECI | CCNNGG | 2 cut(s) | 105, 115 |
| BssMI | GATC | 2 cut(s) | 160, 193 |
| BssNI | GRCGYC | 1 cut(s) | 81 |
| BstACI | GRCGYC | 1 cut(s) | 81 |
| BstC8I | GCNNGC | 4 cut(s) | 149, 183, 303, 316 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstFNI | CGCG | 2 cut(s) | 90, 219 |
| BstHHI | GCGC | 2 cut(s) | 92, 231 |
| BstKTI | GATC | 2 cut(s) | 163, 196 |
| BstMBI | GATC | 2 cut(s) | 160, 193 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 87, 144, 153, 311, 320 |
| BstSCI | CCNGG | 3 cut(s) | 105, 115, 151 |
| BstUI | CGCG | 2 cut(s) | 90, 219 |
| BstV1I | GCAGC | 3 cut(s) | 24, 150, 250 |
| BstV2I | GAAGAC | 1 cut(s) | 302 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BsuRI | GGCC | 3 cut(s) | 110, 156, 308 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| BveI | ACCTGC | 1 cut(s) | 247 |
| Cac8I | GCNNGC | 4 cut(s) | 149, 183, 303, 316 |
| CciI | TCATGA | 1 cut(s) | 270 |
| CfoI | GCGC | 2 cut(s) | 92, 231 |
| Cfr13I | GGNCC | 1 cut(s) | 154 |
| CseI | GACGC | 2 cut(s) | 70, 307 |
| Csp6I | GTAC | 1 cut(s) | 175 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 100, 135 |
| CviAII | CATG | 1 cut(s) | 271 |
| CviJI | RGCY | 8 cut(s) | 110, 156, 181, 242, 248, 263, 308, 318 |
| CviKI_1 | RGCY | 8 cut(s) | 110, 156, 181, 242, 248, 263, 308, 318 |
| CviQI | GTAC | 1 cut(s) | 175 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 2 cut(s) | 162, 195 |
| DpnII | GATC | 2 cut(s) | 160, 193 |
| EaeI | YGGCCR | 2 cut(s) | 108, 306 |
| Eco57I | CTGAAG | 1 cut(s) | 264 |
| FaeI | CATG | 1 cut(s) | 274 |
| FaiI | YATR | 2 cut(s) | 272, 325 |
| FatI | CATG | 1 cut(s) | 270 |
| FauI | CCCGC | 3 cut(s) | 8, 55, 156 |
| Fnu4HI | GCNGC | 5 cut(s) | 10, 13, 139, 264, 306 |
| Fsp4HI | GCNGC | 5 cut(s) | 10, 13, 139, 264, 306 |
| FspBI | CTAG | 2 cut(s) | 6, 171 |
| GlaI | GCGC | 2 cut(s) | 91, 230 |
| GluI | GCNGC | 5 cut(s) | 10, 13, 139, 264, 306 |
| GsaI | CCCAGC | 2 cut(s) | 74, 323 |
| HaeIII | GGCC | 3 cut(s) | 110, 156, 308 |
| HapII | CCGG | 3 cut(s) | 107, 117, 152 |
| HgaI | GACGC | 2 cut(s) | 70, 307 |
| HhaI | GCGC | 2 cut(s) | 92, 231 |
| Hin1I | GRCGYC | 1 cut(s) | 81 |
| Hin1II | CATG | 1 cut(s) | 274 |
| Hin6I | GCGC | 2 cut(s) | 90, 229 |
| HinP1I | GCGC | 2 cut(s) | 90, 229 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HpaII | CCGG | 3 cut(s) | 107, 117, 152 |
| HphI | GGTGA | 1 cut(s) | 134 |
| Hpy166II | GTNNAC | 1 cut(s) | 85 |
| Hpy188I | TCNGA | 1 cut(s) | 67 |
| Hpy188III | TCNNGA | 1 cut(s) | 271 |
| Hpy8I | GTNNAC | 1 cut(s) | 85 |
| HpyCH4IV | ACGT | 1 cut(s) | 277 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 87, 144, 153, 311, 320 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| HpySE526I | ACGT | 1 cut(s) | 277 |
| Hsp92I | GRCGYC | 1 cut(s) | 81 |
| Hsp92II | CATG | 1 cut(s) | 274 |
| HspAI | GCGC | 2 cut(s) | 90, 229 |
| Kzo9I | GATC | 2 cut(s) | 160, 193 |
| LmnI | GCTCC | 1 cut(s) | 226 |
| LpnPI | CCDG | 8 cut(s) | 20, 84, 120, 130, 165, 167, 242, 287 |
| Lsp1109I | GCAGC | 3 cut(s) | 24, 150, 250 |
| MaeI | CTAG | 2 cut(s) | 6, 171 |
| MaeII | ACGT | 1 cut(s) | 277 |
| MaeIII | GTNAC | 1 cut(s) | 166 |
| MalI | GATC | 2 cut(s) | 162, 195 |
| MboI | GATC | 2 cut(s) | 160, 193 |
| MboII | GAAGA | 2 cut(s) | 188, 307 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| MnlI | CCTC | 6 cut(s) | 22, 73, 118, 214, 217, 277 |
| MspA1I | CMGCKG | 2 cut(s) | 15, 74 |
| MspI | CCGG | 3 cut(s) | 107, 117, 152 |
| MspR9I | CCNGG | 3 cut(s) | 107, 117, 153 |
| MvnI | CGCG | 2 cut(s) | 90, 219 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 87, 144, 153, 311, 320 |
| NciI | CCSGG | 3 cut(s) | 107, 117, 153 |
| NdeII | GATC | 2 cut(s) | 160, 193 |
| NlaIII | CATG | 1 cut(s) | 274 |
| NmuCI | GTSAC | 1 cut(s) | 166 |
| PagI | TCATGA | 1 cut(s) | 270 |
| PaqCI | CACCTGC | 1 cut(s) | 247 |
| PkrI | GCNGC | 5 cut(s) | 11, 14, 140, 265, 307 |
| PspFI | CCCAGC | 2 cut(s) | 70, 319 |
| PspPI | GGNCC | 1 cut(s) | 154 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| RsaI | GTAC | 1 cut(s) | 176 |
| RsaNI | GTAC | 1 cut(s) | 175 |
| SatI | GCNGC | 5 cut(s) | 10, 13, 139, 264, 306 |
| Sau3AI | GATC | 2 cut(s) | 160, 193 |
| Sau96I | GGNCC | 1 cut(s) | 154 |
| ScrFI | CCNGG | 3 cut(s) | 107, 117, 153 |
| SetI | ASST | 5 cut(s) | 129, 176, 183, 261, 280 |
| SsiI | CCGC | 8 cut(s) | 9, 15, 48, 74, 88, 149, 305, 312 |
| SspMI | CTAG | 2 cut(s) | 6, 171 |
| StyD4I | CCNGG | 3 cut(s) | 105, 115, 151 |
| TaiI | ACGT | 1 cut(s) | 280 |
| TauI | GCSGC | 2 cut(s) | 12, 308 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseFI | GTSAC | 1 cut(s) | 166 |
| TseI | GCWGC | 3 cut(s) | 12, 138, 263 |
| Tsp45I | GTSAC | 1 cut(s) | 166 |
| TspGWI | ACGGA | 1 cut(s) | 206 |
| TspRI | CASTG | 1 cut(s) | 192 |
| XspI | CTAG | 2 cut(s) | 6, 171 |