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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 556 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 610 |
| AccI | GTMKAC | 1 cut(s) | 358 |
| AccII | CGCG | 2 cut(s) | 74, 90 |
| AclWI | GGATC | 3 cut(s) | 168, 201, 527 |
| AcoI | YGGCCR | 2 cut(s) | 108, 306 |
| AcuI | CTGAAG | 2 cut(s) | 48, 264 |
| AfaI | GTAC | 3 cut(s) | 176, 186, 408 |
| AfiI | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 519, 546, 610 |
| AgsI | TTSAA | 1 cut(s) | 508 |
| AjnI | CCWGG | 7 cut(s) | 133, 151, 156, 347, 382, 421, 512 |
| AluBI | AGCT | 2 cut(s) | 43, 607 |
| AluI | AGCT | 2 cut(s) | 43, 607 |
| Alw21I | GWGCWC | 1 cut(s) | 45 |
| AlwI | GGATC | 3 cut(s) | 168, 201, 527 |
| AoxI | GGCC | 5 cut(s) | 108, 154, 306, 386, 576 |
| ApeKI | GCWGC | 3 cut(s) | 138, 141, 263 |
| AspA2I | CCTAGG | 1 cut(s) | 545 |
| AspLEI | GCGC | 5 cut(s) | 92, 231, 268, 368, 552 |
| AspS9I | GGNCC | 2 cut(s) | 386, 440 |
| AsuC2I | CCSGG | 2 cut(s) | 117, 320 |
| AsuHPI | GGTGA | 4 cut(s) | 134, 160, 337, 542 |
| AvaII | GGWCC | 1 cut(s) | 440 |
| AvrII | CCTAGG | 1 cut(s) | 545 |
| BanII | GRGCYC | 1 cut(s) | 45 |
| Bbv12I | GWGCWC | 1 cut(s) | 45 |
| BbvI | GCAGC | 3 cut(s) | 150, 153, 250 |
| BciT130I | CCWGG | 7 cut(s) | 135, 153, 158, 349, 384, 423, 514 |
| BcnI | CCSGG | 2 cut(s) | 117, 320 |
| BfaI | CTAG | 1 cut(s) | 546 |
| BfoI | RGCGCY | 1 cut(s) | 369 |
| BfuAI | ACCTGC | 1 cut(s) | 556 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 309, 446 |
| BlnI | CCTAGG | 1 cut(s) | 545 |
| BlsI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 307, 310, 447 |
| BmcAI | AGTACT | 1 cut(s) | 186 |
| Bme1390I | CCNGG | 9 cut(s) | 117, 135, 153, 158, 320, 349, 384, 423, 514 |
| Bme18I | GGWCC | 1 cut(s) | 440 |
| BmgT120I | GGNCC | 2 cut(s) | 386, 440 |
| BmiI | GGNNCC | 2 cut(s) | 381, 441 |
| BmrFI | CCNGG | 9 cut(s) | 117, 135, 153, 158, 320, 349, 384, 423, 514 |
| BmsI | GCATC | 3 cut(s) | 351, 583, 609 |
| BpuMI | CCSGG | 2 cut(s) | 117, 320 |
| BsaJI | CCNNGG | 6 cut(s) | 115, 250, 383, 422, 513, 545 |
| Bsc4I | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 519, 546, 610 |
| Bse118I | RCCGGY | 4 cut(s) | 106, 368, 388, 587 |
| Bse1I | ACTGG | 2 cut(s) | 192, 633 |
| BseBI | CCWGG | 7 cut(s) | 135, 153, 158, 349, 384, 423, 514 |
| BseDI | CCNNGG | 6 cut(s) | 115, 250, 383, 422, 513, 545 |
| BseGI | GGATG | 1 cut(s) | 624 |
| BseLI | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 519, 546, 610 |
| BseMII | CTCAG | 2 cut(s) | 516, 575 |
| BseNI | ACTGG | 2 cut(s) | 192, 633 |
| BseRI | GAGGAG | 2 cut(s) | 236, 239 |
| BseX3I | CGGCCG | 1 cut(s) | 306 |
| BseXI | GCAGC | 3 cut(s) | 150, 153, 250 |
| BseYI | CCCAGC | 2 cut(s) | 362, 603 |
| Bsh1236I | CGCG | 2 cut(s) | 74, 90 |
| Bsh1285I | CGRYCG | 2 cut(s) | 78, 309 |
| BshFI | GGCC | 5 cut(s) | 110, 156, 308, 388, 578 |
| BsiEI | CGRYCG | 2 cut(s) | 78, 309 |
| BsiHKAI | GWGCWC | 1 cut(s) | 45 |
| BsiSI | CCGG | 6 cut(s) | 107, 117, 320, 369, 389, 588 |
| BslFI | GGGAC | 1 cut(s) | 453 |
| BslI | CCNNNNNNNGG | 6 cut(s) | 77, 256, 368, 519, 546, 610 |
| BsmFI | GGGAC | 1 cut(s) | 453 |
| BsmI | GAATGC | 1 cut(s) | 514 |
| BsnI | GGCC | 5 cut(s) | 110, 156, 308, 388, 578 |
| Bsp1286I | GDGCHC | 1 cut(s) | 45 |
| Bsp143I | GATC | 4 cut(s) | 75, 160, 193, 532 |
| BspANI | GGCC | 5 cut(s) | 110, 156, 308, 388, 578 |
| BspCNI | CTCAG | 2 cut(s) | 517, 574 |
| BspFNI | CGCG | 2 cut(s) | 74, 90 |
| BspLI | GGNNCC | 2 cut(s) | 381, 441 |
| BspMI | ACCTGC | 1 cut(s) | 556 |
| BspPI | GGATC | 3 cut(s) | 168, 201, 527 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BsrFI | RCCGGY | 4 cut(s) | 106, 368, 388, 587 |
| BsrI | ACTGG | 2 cut(s) | 192, 633 |
| BssAI | RCCGGY | 4 cut(s) | 106, 368, 388, 587 |
| BssECI | CCNNGG | 6 cut(s) | 115, 250, 383, 422, 513, 545 |
| BssMI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| BssT1I | CCWWGG | 2 cut(s) | 250, 545 |
| Bst2UI | CCWGG | 7 cut(s) | 135, 153, 158, 349, 384, 423, 514 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 7 cut(s) | 36, 149, 332, 370, 495, 554, 589 |
| BstDEI | CTNAG | 2 cut(s) | 525, 561 |
| BstF5I | GGATG | 1 cut(s) | 624 |
| BstFNI | CGCG | 2 cut(s) | 74, 90 |
| BstH2I | RGCGCY | 1 cut(s) | 369 |
| BstHHI | GCGC | 5 cut(s) | 92, 231, 268, 368, 552 |
| BstKTI | GATC | 4 cut(s) | 78, 163, 196, 535 |
| BstMBI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| BstMCI | CGRYCG | 2 cut(s) | 78, 309 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 40, 87, 144, 153, 272, 409, 562, 584, 593 |
| BstNI | CCWGG | 7 cut(s) | 135, 153, 158, 349, 384, 423, 514 |
| BstNSI | RCATGY | 1 cut(s) | 407 |
| BstSCI | CCNGG | 9 cut(s) | 115, 133, 151, 156, 318, 347, 382, 421, 512 |
| BstUI | CGCG | 2 cut(s) | 74, 90 |
| BstV1I | GCAGC | 3 cut(s) | 150, 153, 250 |
| BstX2I | RGATCY | 2 cut(s) | 160, 193 |
| BstYI | RGATCY | 2 cut(s) | 160, 193 |
| BstZI | CGGCCG | 1 cut(s) | 306 |
| BsuRI | GGCC | 5 cut(s) | 110, 156, 308, 388, 578 |
| BtgZI | GCGATG | 1 cut(s) | 606 |
| BtsCI | GGATG | 1 cut(s) | 624 |
| BveI | ACCTGC | 1 cut(s) | 556 |
| Cac8I | GCNNGC | 7 cut(s) | 36, 149, 332, 370, 495, 554, 589 |
| CciNI | GCGGCCGC | 1 cut(s) | 306 |
| CfoI | GCGC | 5 cut(s) | 92, 231, 268, 368, 552 |
| Cfr10I | RCCGGY | 4 cut(s) | 106, 368, 388, 587 |
| Cfr13I | GGNCC | 2 cut(s) | 386, 440 |
| Csp6I | GTAC | 3 cut(s) | 175, 185, 407 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 408, 443 |
| CviAII | CATG | 3 cut(s) | 172, 271, 404 |
| CviQI | GTAC | 3 cut(s) | 175, 185, 407 |
| DdeI | CTNAG | 2 cut(s) | 525, 561 |
| DpnI | GATC | 4 cut(s) | 77, 162, 195, 534 |
| DpnII | GATC | 4 cut(s) | 75, 160, 193, 532 |
| EaeI | YGGCCR | 2 cut(s) | 108, 306 |
| EagI | CGGCCG | 1 cut(s) | 306 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| EciI | GGCGGA | 4 cut(s) | 53, 206, 525, 574 |
| Ecl136II | GAGCTC | 1 cut(s) | 43 |
| EclXI | CGGCCG | 1 cut(s) | 306 |
| Eco130I | CCWWGG | 2 cut(s) | 250, 545 |
| Eco24I | GRGCYC | 1 cut(s) | 45 |
| Eco47I | GGWCC | 1 cut(s) | 440 |
| Eco52I | CGGCCG | 1 cut(s) | 306 |
| Eco53kI | GAGCTC | 1 cut(s) | 43 |
| Eco57I | CTGAAG | 2 cut(s) | 48, 264 |
| EcoICRI | GAGCTC | 1 cut(s) | 43 |
| EcoRII | CCWGG | 7 cut(s) | 133, 151, 156, 347, 382, 421, 512 |
| EcoT14I | CCWWGG | 2 cut(s) | 250, 545 |
| EcoT22I | ATGCAT | 1 cut(s) | 405 |
| EcoT38I | GRGCYC | 1 cut(s) | 45 |
| ErhI | CCWWGG | 2 cut(s) | 250, 545 |
| FaeI | CATG | 3 cut(s) | 175, 274, 407 |
| FaiI | YATR | 7 cut(s) | 173, 201, 272, 395, 405, 452, 542 |
| FaqI | GGGAC | 1 cut(s) | 453 |
| FatI | CATG | 3 cut(s) | 171, 270, 403 |
| FauI | CCCGC | 4 cut(s) | 8, 55, 79, 156 |
| FblI | GTMKAC | 1 cut(s) | 358 |
| Fnu4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 309, 446 |
| FokI | GGATG | 1 cut(s) | 631 |
| FriOI | GRGCYC | 1 cut(s) | 45 |
| Fsp4HI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 309, 446 |
| FspBI | CTAG | 1 cut(s) | 546 |
| GlaI | GCGC | 5 cut(s) | 91, 230, 267, 367, 551 |
| GluI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 309, 446 |
| GsaI | CCCAGC | 2 cut(s) | 366, 607 |
| HaeII | RGCGCY | 1 cut(s) | 369 |
| HaeIII | GGCC | 5 cut(s) | 110, 156, 308, 388, 578 |
| HapII | CCGG | 6 cut(s) | 107, 117, 320, 369, 389, 588 |
| HhaI | GCGC | 5 cut(s) | 92, 231, 268, 368, 552 |
| Hin1II | CATG | 3 cut(s) | 175, 274, 407 |
| Hin6I | GCGC | 5 cut(s) | 90, 229, 266, 366, 550 |
| HinP1I | GCGC | 5 cut(s) | 90, 229, 266, 366, 550 |
| HincII | GTYRAC | 2 cut(s) | 85, 359 |
| HindII | GTYRAC | 2 cut(s) | 85, 359 |
| HinfI | GANTC | 1 cut(s) | 581 |
| HpaI | GTTAAC | 1 cut(s) | 85 |
| HpaII | CCGG | 6 cut(s) | 107, 117, 320, 369, 389, 588 |
| HphI | GGTGA | 4 cut(s) | 134, 160, 337, 542 |
| Hpy166II | GTNNAC | 2 cut(s) | 85, 359 |
| Hpy188I | TCNGA | 1 cut(s) | 67 |
| Hpy188III | TCNNGA | 1 cut(s) | 432 |
| Hpy8I | GTNNAC | 2 cut(s) | 85, 359 |
| HpyAV | CCTTC | 1 cut(s) | 232 |
| HpyCH4IV | ACGT | 1 cut(s) | 217 |
| HpyCH4V | TGCA | 3 cut(s) | 403, 596, 622 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 40, 87, 144, 153, 272, 409, 562, 584, 593 |
| HpyF3I | CTNAG | 2 cut(s) | 525, 561 |
| HpySE526I | ACGT | 1 cut(s) | 217 |
| Hsp92II | CATG | 3 cut(s) | 175, 274, 407 |
| HspAI | GCGC | 5 cut(s) | 90, 229, 266, 366, 550 |
| KroI | GCCGGC | 2 cut(s) | 368, 587 |
| KroNI | GCCGGC | 2 cut(s) | 370, 589 |
| KspAI | GTTAAC | 1 cut(s) | 85 |
| Kzo9I | GATC | 4 cut(s) | 75, 160, 193, 532 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LmnI | GCTCC | 5 cut(s) | 40, 48, 226, 327, 379 |
| Lsp1109I | GCAGC | 3 cut(s) | 150, 153, 250 |
| LweI | GCATC | 3 cut(s) | 351, 583, 609 |
| MaeI | CTAG | 1 cut(s) | 546 |
| MaeII | ACGT | 1 cut(s) | 217 |
| MaeIII | GTNAC | 2 cut(s) | 166, 336 |
| MalI | GATC | 4 cut(s) | 77, 162, 195, 534 |
| MboI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| MboII | GAAGA | 2 cut(s) | 41, 188 |
| MflI | RGATCY | 2 cut(s) | 160, 193 |
| MhlI | GDGCHC | 1 cut(s) | 45 |
| MmeI | TCCRAC | 1 cut(s) | 90 |
| MnlI | CCTC | 6 cut(s) | 73, 118, 214, 217, 277, 520 |
| Mph1103I | ATGCAT | 1 cut(s) | 405 |
| MreI | CGCCGGCG | 1 cut(s) | 587 |
| MroNI | GCCGGC | 2 cut(s) | 368, 587 |
| MseI | TTAA | 1 cut(s) | 84 |
| MslI | CAYNNNNRTG | 1 cut(s) | 398 |
| MspA1I | CMGCKG | 2 cut(s) | 15, 412 |
| MspI | CCGG | 6 cut(s) | 107, 117, 320, 369, 389, 588 |
| MspR9I | CCNGG | 9 cut(s) | 117, 135, 153, 158, 320, 349, 384, 423, 514 |
| Mva1269I | GAATGC | 1 cut(s) | 514 |
| MvaI | CCWGG | 7 cut(s) | 135, 153, 158, 349, 384, 423, 514 |
| MvnI | CGCG | 2 cut(s) | 74, 90 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 40, 87, 144, 153, 272, 409, 562, 584, 593 |
| NaeI | GCCGGC | 2 cut(s) | 370, 589 |
| NciI | CCSGG | 2 cut(s) | 117, 320 |
| NdeII | GATC | 4 cut(s) | 75, 160, 193, 532 |
| NgoMIV | GCCGGC | 2 cut(s) | 368, 587 |
| NlaIII | CATG | 3 cut(s) | 175, 274, 407 |
| NlaIV | GGNNCC | 2 cut(s) | 381, 441 |
| NmuCI | GTSAC | 2 cut(s) | 166, 336 |
| NotI | GCGGCCGC | 1 cut(s) | 306 |
| NsiI | ATGCAT | 1 cut(s) | 405 |
| NspI | RCATGY | 1 cut(s) | 407 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PctI | GAATGC | 1 cut(s) | 514 |
| PdiI | GCCGGC | 2 cut(s) | 370, 589 |
| PfeI | GAWTC | 1 cut(s) | 581 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 610 |
| PkrI | GCNGC | 7 cut(s) | 140, 143, 146, 265, 307, 310, 447 |
| Ple19I | CGATCG | 1 cut(s) | 78 |
| Psp124BI | GAGCTC | 1 cut(s) | 45 |
| Psp6I | CCWGG | 7 cut(s) | 133, 151, 156, 347, 382, 421, 512 |
| PspFI | CCCAGC | 2 cut(s) | 362, 603 |
| PspGI | CCWGG | 7 cut(s) | 133, 151, 156, 347, 382, 421, 512 |
| PspN4I | GGNNCC | 2 cut(s) | 381, 441 |
| PspPI | GGNCC | 2 cut(s) | 386, 440 |
| PsuI | RGATCY | 2 cut(s) | 160, 193 |
| PvuI | CGATCG | 1 cut(s) | 78 |
| RsaI | GTAC | 3 cut(s) | 176, 186, 408 |
| RsaNI | GTAC | 3 cut(s) | 175, 185, 407 |
| RseI | CAYNNNNRTG | 1 cut(s) | 398 |
| SacI | GAGCTC | 1 cut(s) | 45 |
| SalI | GTCGAC | 1 cut(s) | 357 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SaqAI | TTAA | 1 cut(s) | 84 |
| SatI | GCNGC | 7 cut(s) | 139, 142, 145, 264, 306, 309, 446 |
| Sau3AI | GATC | 4 cut(s) | 75, 160, 193, 532 |
| Sau96I | GGNCC | 2 cut(s) | 386, 440 |
| ScaI | AGTACT | 1 cut(s) | 186 |
| ScrFI | CCNGG | 9 cut(s) | 117, 135, 153, 158, 320, 349, 384, 423, 514 |
| SduI | GDGCHC | 1 cut(s) | 45 |
| SetI | ASST | 8 cut(s) | 45, 220, 261, 424, 477, 547, 570, 609 |
| SfaNI | GCATC | 3 cut(s) | 351, 583, 609 |
| SgrAI | CRCCGGYG | 1 cut(s) | 587 |
| SinI | GGWCC | 1 cut(s) | 440 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 398 |
| SspMI | CTAG | 1 cut(s) | 546 |
| SstI | GAGCTC | 1 cut(s) | 45 |
| StyD4I | CCNGG | 9 cut(s) | 115, 133, 151, 156, 318, 347, 382, 421, 512 |
| StyI | CCWWGG | 2 cut(s) | 250, 545 |
| TaiI | ACGT | 1 cut(s) | 220 |
| TaqI | TCGA | 1 cut(s) | 358 |
| TatI | WGTACW | 1 cut(s) | 184 |
| TauI | GCSGC | 4 cut(s) | 147, 308, 311, 448 |
| TfiI | GAWTC | 1 cut(s) | 581 |
| Tru1I | TTAA | 1 cut(s) | 84 |
| Tru9I | TTAA | 1 cut(s) | 84 |
| TseFI | GTSAC | 2 cut(s) | 166, 336 |
| TseI | GCWGC | 3 cut(s) | 138, 141, 263 |
| Tsp45I | GTSAC | 2 cut(s) | 166, 336 |
| TspDTI | ATGAA | 1 cut(s) | 188 |
| TspGWI | ACGGA | 1 cut(s) | 340 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 610 |
| VpaK11BI | GGWCC | 1 cut(s) | 440 |
| XceI | RCATGY | 1 cut(s) | 407 |
| XmaJI | CCTAGG | 1 cut(s) | 545 |
| XmiI | GTMKAC | 1 cut(s) | 358 |
| XspI | CTAG | 1 cut(s) | 546 |
| ZrmI | AGTACT | 1 cut(s) | 186 |
| Zsp2I | ATGCAT | 1 cut(s) | 405 |