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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 445 |
| Acc36I | ACCTGC | 1 cut(s) | 445 |
| AccII | CGCG | 2 cut(s) | 109, 119 |
| AciI | CCGC | 5 cut(s) | 21, 82, 112, 302, 389 |
| AclWI | GGATC | 1 cut(s) | 103 |
| AcoI | YGGCCR | 1 cut(s) | 459 |
| AcsI | RAATTY | 1 cut(s) | 476 |
| AcuI | CTGAAG | 1 cut(s) | 182 |
| AfaI | GTAC | 1 cut(s) | 31 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 45, 221, 400, 454 |
| AflIII | ACRYGT | 1 cut(s) | 117 |
| AgsI | TTSAA | 1 cut(s) | 266 |
| AjnI | CCWGG | 4 cut(s) | 69, 91, 282, 349 |
| AluBI | AGCT | 3 cut(s) | 144, 153, 427 |
| AluI | AGCT | 3 cut(s) | 144, 153, 427 |
| Alw21I | GWGCWC | 2 cut(s) | 260, 429 |
| Alw26I | GTCTC | 1 cut(s) | 317 |
| AlwI | GGATC | 1 cut(s) | 103 |
| AoxI | GGCC | 3 cut(s) | 223, 444, 459 |
| ApoI | RAATTY | 1 cut(s) | 476 |
| AspLEI | GCGC | 2 cut(s) | 111, 500 |
| AspS9I | GGNCC | 2 cut(s) | 391, 445 |
| AsuC2I | CCSGG | 1 cut(s) | 333 |
| AsuHPI | GGTGA | 2 cut(s) | 90, 235 |
| AvaII | GGWCC | 1 cut(s) | 391 |
| BalI | TGGCCA | 1 cut(s) | 461 |
| BanII | GRGCYC | 2 cut(s) | 92, 429 |
| Bbv12I | GWGCWC | 2 cut(s) | 260, 429 |
| BciT130I | CCWGG | 4 cut(s) | 71, 93, 284, 351 |
| BcnI | CCSGG | 1 cut(s) | 333 |
| BcoDI | GTCTC | 1 cut(s) | 317 |
| BfoI | RGCGCY | 1 cut(s) | 501 |
| BfuAI | ACCTGC | 1 cut(s) | 445 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 75 |
| BglII | AGATCT | 1 cut(s) | 358 |
| BisI | GCNGC | 1 cut(s) | 112 |
| BlsI | GCNGC | 1 cut(s) | 113 |
| Bme1390I | CCNGG | 5 cut(s) | 71, 93, 284, 333, 351 |
| Bme18I | GGWCC | 1 cut(s) | 391 |
| BmgT120I | GGNCC | 2 cut(s) | 391, 445 |
| BmrFI | CCNGG | 5 cut(s) | 71, 93, 284, 333, 351 |
| BmsI | GCATC | 1 cut(s) | 144 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 27 |
| BpuEI | CTTGAG | 2 cut(s) | 106, 130 |
| BpuMI | CCSGG | 1 cut(s) | 333 |
| BsaI | GGTCTC | 1 cut(s) | 317 |
| BsaJI | CCNNGG | 3 cut(s) | 91, 282, 283 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 45, 221, 400, 454 |
| BseBI | CCWGG | 4 cut(s) | 71, 93, 284, 351 |
| BseDI | CCNNGG | 3 cut(s) | 91, 282, 283 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 45, 221, 400, 454 |
| BseMII | CTCAG | 1 cut(s) | 442 |
| BseRI | GAGGAG | 1 cut(s) | 208 |
| BseYI | CCCAGC | 1 cut(s) | 153 |
| Bsh1236I | CGCG | 2 cut(s) | 109, 119 |
| BshFI | GGCC | 3 cut(s) | 225, 446, 461 |
| BsiHKAI | GWGCWC | 2 cut(s) | 260, 429 |
| BsiSI | CCGG | 1 cut(s) | 332 |
| BslFI | GGGAC | 2 cut(s) | 159, 284 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 45, 221, 400, 454 |
| BsmAI | GTCTC | 1 cut(s) | 317 |
| BsmFI | GGGAC | 2 cut(s) | 159, 284 |
| BsnI | GGCC | 3 cut(s) | 225, 446, 461 |
| Bso31I | GGTCTC | 1 cut(s) | 317 |
| Bsp1286I | GDGCHC | 3 cut(s) | 92, 260, 429 |
| Bsp143I | GATC | 2 cut(s) | 95, 358 |
| BspACI | CCGC | 5 cut(s) | 21, 82, 112, 302, 389 |
| BspANI | GGCC | 3 cut(s) | 225, 446, 461 |
| BspCNI | CTCAG | 1 cut(s) | 441 |
| BspFNI | CGCG | 2 cut(s) | 109, 119 |
| BspMI | ACCTGC | 1 cut(s) | 445 |
| BspPI | GGATC | 1 cut(s) | 103 |
| BspTNI | GGTCTC | 1 cut(s) | 317 |
| BssECI | CCNNGG | 3 cut(s) | 91, 282, 283 |
| BssMI | GATC | 2 cut(s) | 95, 358 |
| Bst2UI | CCWGG | 4 cut(s) | 71, 93, 284, 351 |
| Bst6I | CTCTTC | 1 cut(s) | 211 |
| BstC8I | GCNNGC | 2 cut(s) | 25, 76 |
| BstDEI | CTNAG | 2 cut(s) | 252, 428 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 219, 398 |
| BstFNI | CGCG | 2 cut(s) | 109, 119 |
| BstH2I | RGCGCY | 1 cut(s) | 501 |
| BstHHI | GCGC | 2 cut(s) | 111, 500 |
| BstKTI | GATC | 2 cut(s) | 98, 361 |
| BstMAI | GTCTC | 1 cut(s) | 317 |
| BstMBI | GATC | 2 cut(s) | 95, 358 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 75, 141, 150, 342, 504 |
| BstNI | CCWGG | 4 cut(s) | 71, 93, 284, 351 |
| BstSCI | CCNGG | 5 cut(s) | 69, 91, 282, 331, 349 |
| BstUI | CGCG | 2 cut(s) | 109, 119 |
| BstX2I | RGATCY | 1 cut(s) | 358 |
| BstYI | RGATCY | 1 cut(s) | 358 |
| BsuRI | GGCC | 3 cut(s) | 225, 446, 461 |
| BtsIMutI | CAGTG | 1 cut(s) | 464 |
| BveI | ACCTGC | 1 cut(s) | 445 |
| Cac8I | GCNNGC | 2 cut(s) | 25, 76 |
| CfoI | GCGC | 2 cut(s) | 111, 500 |
| Cfr13I | GGNCC | 2 cut(s) | 391, 445 |
| CseI | GACGC | 2 cut(s) | 108, 115 |
| CsiI | ACCWGGT | 1 cut(s) | 349 |
| Csp6I | GTAC | 1 cut(s) | 30 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 104, 139 |
| CviAII | CATG | 3 cut(s) | 14, 189, 247 |
| CviQI | GTAC | 1 cut(s) | 30 |
| DdeI | CTNAG | 2 cut(s) | 252, 428 |
| DpnI | GATC | 2 cut(s) | 97, 360 |
| DpnII | GATC | 2 cut(s) | 95, 358 |
| EaeI | YGGCCR | 1 cut(s) | 459 |
| Eam1104I | CTCTTC | 1 cut(s) | 211 |
| EarI | CTCTTC | 1 cut(s) | 211 |
| EciI | GGCGGA | 1 cut(s) | 404 |
| Ecl136II | GAGCTC | 1 cut(s) | 427 |
| Eco24I | GRGCYC | 2 cut(s) | 92, 429 |
| Eco31I | GGTCTC | 1 cut(s) | 317 |
| Eco47I | GGWCC | 1 cut(s) | 391 |
| Eco53kI | GAGCTC | 1 cut(s) | 427 |
| Eco57I | CTGAAG | 1 cut(s) | 182 |
| EcoICRI | GAGCTC | 1 cut(s) | 427 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 219, 398 |
| EcoRII | CCWGG | 4 cut(s) | 69, 91, 282, 349 |
| EcoT38I | GRGCYC | 2 cut(s) | 92, 429 |
| FaeI | CATG | 3 cut(s) | 17, 192, 250 |
| FaiI | YATR | 7 cut(s) | 15, 168, 190, 218, 248, 371, 375 |
| FaqI | GGGAC | 2 cut(s) | 159, 284 |
| FatI | CATG | 3 cut(s) | 13, 188, 246 |
| FauI | CCCGC | 1 cut(s) | 309 |
| Fnu4HI | GCNGC | 1 cut(s) | 112 |
| FriOI | GRGCYC | 2 cut(s) | 92, 429 |
| Fsp4HI | GCNGC | 1 cut(s) | 112 |
| GlaI | GCGC | 2 cut(s) | 110, 499 |
| GluI | GCNGC | 1 cut(s) | 112 |
| GsaI | CCCAGC | 1 cut(s) | 157 |
| HaeII | RGCGCY | 1 cut(s) | 501 |
| HaeIII | GGCC | 3 cut(s) | 225, 446, 461 |
| HapII | CCGG | 1 cut(s) | 332 |
| HgaI | GACGC | 2 cut(s) | 108, 115 |
| HhaI | GCGC | 2 cut(s) | 111, 500 |
| Hin1II | CATG | 3 cut(s) | 17, 192, 250 |
| Hin6I | GCGC | 2 cut(s) | 109, 498 |
| HinP1I | GCGC | 2 cut(s) | 109, 498 |
| HincII | GTYRAC | 1 cut(s) | 278 |
| HindII | GTYRAC | 1 cut(s) | 278 |
| HinfI | GANTC | 1 cut(s) | 307 |
| HpaII | CCGG | 1 cut(s) | 332 |
| HphI | GGTGA | 2 cut(s) | 90, 235 |
| Hpy166II | GTNNAC | 1 cut(s) | 278 |
| Hpy188I | TCNGA | 1 cut(s) | 431 |
| Hpy188III | TCNNGA | 2 cut(s) | 147, 356 |
| Hpy8I | GTNNAC | 1 cut(s) | 278 |
| Hpy99I | CGWCG | 2 cut(s) | 368, 485 |
| HpyAV | CCTTC | 1 cut(s) | 430 |
| HpyCH4V | TGCA | 1 cut(s) | 317 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 75, 141, 150, 342, 504 |
| HpyF3I | CTNAG | 2 cut(s) | 252, 428 |
| Hsp92II | CATG | 3 cut(s) | 17, 192, 250 |
| HspAI | GCGC | 2 cut(s) | 109, 498 |
| Kzo9I | GATC | 2 cut(s) | 95, 358 |
| LweI | GCATC | 1 cut(s) | 144 |
| MabI | ACCWGGT | 1 cut(s) | 349 |
| MaeIII | GTNAC | 1 cut(s) | 50 |
| MalI | GATC | 2 cut(s) | 97, 360 |
| MboI | GATC | 2 cut(s) | 95, 358 |
| MboII | GAAGA | 1 cut(s) | 198 |
| MflI | RGATCY | 1 cut(s) | 358 |
| MhlI | GDGCHC | 3 cut(s) | 92, 260, 429 |
| MlsI | TGGCCA | 1 cut(s) | 461 |
| MluCI | AATT | 1 cut(s) | 476 |
| MluI | ACGCGT | 1 cut(s) | 117 |
| MluNI | TGGCCA | 1 cut(s) | 461 |
| MlyI | GAGTC | 1 cut(s) | 301 |
| MnlI | CCTC | 7 cut(s) | 186, 189, 214, 404, 406, 436, 497 |
| Mox20I | TGGCCA | 1 cut(s) | 461 |
| MscI | TGGCCA | 1 cut(s) | 461 |
| MslI | CAYNNNNRTG | 2 cut(s) | 12, 467 |
| Msp20I | TGGCCA | 1 cut(s) | 461 |
| MspA1I | CMGCKG | 1 cut(s) | 304 |
| MspI | CCGG | 1 cut(s) | 332 |
| MspR9I | CCNGG | 5 cut(s) | 71, 93, 284, 333, 351 |
| MvaI | CCWGG | 4 cut(s) | 71, 93, 284, 351 |
| MvnI | CGCG | 2 cut(s) | 109, 119 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 75, 141, 150, 342, 504 |
| NciI | CCSGG | 1 cut(s) | 333 |
| NdeII | GATC | 2 cut(s) | 95, 358 |
| NlaIII | CATG | 3 cut(s) | 17, 192, 250 |
| NmuCI | GTSAC | 1 cut(s) | 50 |
| PaqCI | CACCTGC | 1 cut(s) | 445 |
| PasI | CCCWGGG | 1 cut(s) | 283 |
| PkrI | GCNGC | 1 cut(s) | 113 |
| PleI | GAGTC | 1 cut(s) | 301 |
| PpsI | GAGTC | 1 cut(s) | 301 |
| Psp124BI | GAGCTC | 1 cut(s) | 429 |
| Psp6I | CCWGG | 4 cut(s) | 69, 91, 282, 349 |
| PspFI | CCCAGC | 1 cut(s) | 153 |
| PspGI | CCWGG | 4 cut(s) | 69, 91, 282, 349 |
| PspPI | GGNCC | 2 cut(s) | 391, 445 |
| PsuI | RGATCY | 1 cut(s) | 358 |
| RsaI | GTAC | 1 cut(s) | 31 |
| RsaNI | GTAC | 1 cut(s) | 30 |
| RseI | CAYNNNNRTG | 2 cut(s) | 12, 467 |
| SacI | GAGCTC | 1 cut(s) | 429 |
| SatI | GCNGC | 1 cut(s) | 112 |
| Sau3AI | GATC | 2 cut(s) | 95, 358 |
| Sau96I | GGNCC | 2 cut(s) | 391, 445 |
| SchI | GAGTC | 1 cut(s) | 301 |
| ScrFI | CCNGG | 5 cut(s) | 71, 93, 284, 333, 351 |
| SduI | GDGCHC | 3 cut(s) | 92, 260, 429 |
| SetI | ASST | 9 cut(s) | 57, 146, 155, 200, 217, 355, 396, 429, 459 |
| SexAI | ACCWGGT | 1 cut(s) | 349 |
| SfaNI | GCATC | 1 cut(s) | 144 |
| SinI | GGWCC | 1 cut(s) | 391 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 12, 467 |
| SmlI | CTYRAG | 2 cut(s) | 85, 145 |
| SmoI | CTYRAG | 2 cut(s) | 85, 145 |
| Sse9I | AATT | 1 cut(s) | 476 |
| SsiI | CCGC | 5 cut(s) | 21, 82, 112, 302, 389 |
| SstI | GAGCTC | 1 cut(s) | 429 |
| StyD4I | CCNGG | 5 cut(s) | 69, 91, 282, 331, 349 |
| TaqI | TCGA | 2 cut(s) | 182, 480 |
| TasI | AATT | 1 cut(s) | 476 |
| TauI | GCSGC | 1 cut(s) | 114 |
| TscAI | CASTG | 1 cut(s) | 471 |
| TseFI | GTSAC | 1 cut(s) | 50 |
| Tsp45I | GTSAC | 1 cut(s) | 50 |
| TspGWI | ACGGA | 3 cut(s) | 61, 73, 335 |
| TspRI | CASTG | 1 cut(s) | 471 |
| VpaK11BI | GGWCC | 1 cut(s) | 391 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 219, 398 |
| XapI | RAATTY | 1 cut(s) | 476 |