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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 377 |
| Acc65I | GGTACC | 2 cut(s) | 362, 389 |
| AccB1I | GGYRCC | 3 cut(s) | 348, 362, 389 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 593 |
| AccBSI | CCGCTC | 2 cut(s) | 487, 668 |
| AccI | GTMKAC | 1 cut(s) | 341 |
| AccII | CGCG | 1 cut(s) | 73 |
| AclWI | GGATC | 3 cut(s) | 184, 620, 765 |
| AcuI | CTGAAG | 2 cut(s) | 31, 247 |
| AcyI | GRCGYC | 1 cut(s) | 643 |
| AdeI | CACNNNGTG | 1 cut(s) | 312 |
| AfaI | GTAC | 4 cut(s) | 159, 364, 379, 391 |
| AfiI | CCNNNNNNNGG | 7 cut(s) | 60, 134, 239, 351, 491, 593, 685 |
| AflIII | ACRYGT | 1 cut(s) | 258 |
| AgsI | TTSAA | 1 cut(s) | 797 |
| AjnI | CCWGG | 3 cut(s) | 330, 365, 683 |
| AluBI | AGCT | 1 cut(s) | 164 |
| AluI | AGCT | 1 cut(s) | 164 |
| Alw26I | GTCTC | 1 cut(s) | 754 |
| AlwI | GGATC | 3 cut(s) | 184, 620, 765 |
| AoxI | GGCC | 5 cut(s) | 137, 289, 369, 537, 687 |
| ApaI | GGGCCC | 1 cut(s) | 691 |
| ApeKI | GCWGC | 3 cut(s) | 121, 124, 699 |
| Asp718I | GGTACC | 2 cut(s) | 362, 389 |
| AspLEI | GCGC | 4 cut(s) | 75, 214, 535, 653 |
| AspS9I | GGNCC | 5 cut(s) | 137, 369, 423, 687, 688 |
| AsuC2I | CCSGG | 3 cut(s) | 100, 346, 630 |
| AsuHPI | GGTGA | 3 cut(s) | 117, 686, 797 |
| AsuNHI | GCTAGC | 1 cut(s) | 164 |
| AvaII | GGWCC | 1 cut(s) | 423 |
| BaeGI | GKGCMC | 1 cut(s) | 691 |
| BaeI | ACNNNNGTAYC | 4 cut(s) | 346, 373, 379, 406 |
| BanI | GGYRCC | 3 cut(s) | 348, 362, 389 |
| BanII | GRGCYC | 1 cut(s) | 691 |
| BbsI | GAAGAC | 1 cut(s) | 285 |
| BbvI | GCAGC | 3 cut(s) | 133, 136, 686 |
| BccI | CCATC | 1 cut(s) | 42 |
| BceAI | ACGGC | 2 cut(s) | 524, 773 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 564, 598 |
| BciT130I | CCWGG | 3 cut(s) | 332, 367, 685 |
| BcnI | CCSGG | 3 cut(s) | 100, 346, 630 |
| BcoDI | GTCTC | 1 cut(s) | 754 |
| BfaI | CTAG | 3 cut(s) | 165, 496, 544 |
| BfmI | CTRYAG | 1 cut(s) | 708 |
| BfoI | RGCGCY | 1 cut(s) | 654 |
| BfuAI | ACCTGC | 1 cut(s) | 377 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 136, 545 |
| BglII | AGATCT | 1 cut(s) | 143 |
| BisI | GCNGC | 4 cut(s) | 122, 125, 128, 700 |
| BlsI | GCNGC | 4 cut(s) | 123, 126, 129, 701 |
| Bme1390I | CCNGG | 6 cut(s) | 100, 332, 346, 367, 630, 685 |
| Bme18I | GGWCC | 1 cut(s) | 423 |
| BmgT120I | GGNCC | 5 cut(s) | 137, 369, 423, 687, 688 |
| BmiI | GGNNCC | 5 cut(s) | 350, 364, 391, 424, 689 |
| BmrFI | CCNGG | 6 cut(s) | 100, 332, 346, 367, 630, 685 |
| BmsI | GCATC | 2 cut(s) | 566, 592 |
| BmtI | GCTAGC | 1 cut(s) | 168 |
| BpiI | GAAGAC | 1 cut(s) | 285 |
| BpmI | CTGGAG | 1 cut(s) | 306 |
| BpuMI | CCSGG | 3 cut(s) | 100, 346, 630 |
| BsaHI | GRCGYC | 1 cut(s) | 643 |
| BsaJI | CCNNGG | 4 cut(s) | 98, 366, 528, 684 |
| Bsc4I | CCNNNNNNNGG | 7 cut(s) | 60, 134, 239, 351, 491, 593, 685 |
| Bse118I | RCCGGY | 3 cut(s) | 351, 371, 722 |
| Bse1I | ACTGG | 1 cut(s) | 175 |
| BseBI | CCWGG | 3 cut(s) | 332, 367, 685 |
| BseDI | CCNNGG | 4 cut(s) | 98, 366, 528, 684 |
| BseGI | GGATG | 3 cut(s) | 26, 34, 607 |
| BseLI | CCNNNNNNNGG | 7 cut(s) | 60, 134, 239, 351, 491, 593, 685 |
| BseMII | CTCAG | 4 cut(s) | 397, 499, 502, 672 |
| BseNI | ACTGG | 1 cut(s) | 175 |
| BseRI | GAGGAG | 2 cut(s) | 219, 222 |
| BseSI | GKGCMC | 1 cut(s) | 691 |
| BseXI | GCAGC | 3 cut(s) | 133, 136, 686 |
| BseYI | CCCAGC | 3 cut(s) | 53, 302, 647 |
| Bsh1236I | CGCG | 1 cut(s) | 73 |
| Bsh1285I | CGRYCG | 1 cut(s) | 376 |
| BshFI | GGCC | 5 cut(s) | 139, 291, 371, 539, 689 |
| BshNI | GGYRCC | 3 cut(s) | 348, 362, 389 |
| BsiEI | CGRYCG | 1 cut(s) | 376 |
| BsiSI | CCGG | 6 cut(s) | 100, 346, 352, 372, 629, 723 |
| BslFI | GGGAC | 2 cut(s) | 436, 630 |
| BslI | CCNNNNNNNGG | 7 cut(s) | 60, 134, 239, 351, 491, 593, 685 |
| BsmAI | GTCTC | 1 cut(s) | 754 |
| BsmFI | GGGAC | 2 cut(s) | 436, 630 |
| BsnI | GGCC | 5 cut(s) | 139, 291, 371, 539, 689 |
| Bsp120I | GGGCCC | 1 cut(s) | 687 |
| Bsp1286I | GDGCHC | 1 cut(s) | 691 |
| Bsp143I | GATC | 4 cut(s) | 143, 176, 625, 770 |
| BspANI | GGCC | 5 cut(s) | 139, 291, 371, 539, 689 |
| BspCNI | CTCAG | 4 cut(s) | 398, 500, 501, 671 |
| BspFNI | CGCG | 1 cut(s) | 73 |
| BspHI | TCATGA | 1 cut(s) | 253 |
| BspLI | GGNNCC | 5 cut(s) | 350, 364, 391, 424, 689 |
| BspMAI | CTGCAG | 1 cut(s) | 712 |
| BspMI | ACCTGC | 1 cut(s) | 377 |
| BspOI | GCTAGC | 1 cut(s) | 168 |
| BspPI | GGATC | 3 cut(s) | 184, 620, 765 |
| BspQI | GCTCTTC | 2 cut(s) | 7, 675 |
| BspT107I | GGYRCC | 3 cut(s) | 348, 362, 389 |
| BsrBI | CCGCTC | 2 cut(s) | 487, 668 |
| BsrFI | RCCGGY | 3 cut(s) | 351, 371, 722 |
| BsrI | ACTGG | 1 cut(s) | 175 |
| BssAI | RCCGGY | 3 cut(s) | 351, 371, 722 |
| BssECI | CCNNGG | 4 cut(s) | 98, 366, 528, 684 |
| BssMI | GATC | 4 cut(s) | 143, 176, 625, 770 |
| BssNI | GRCGYC | 1 cut(s) | 643 |
| BssT1I | CCWWGG | 1 cut(s) | 528 |
| Bst2UI | CCWGG | 3 cut(s) | 332, 367, 685 |
| Bst6I | CTCTTC | 2 cut(s) | 7, 675 |
| BstACI | GRCGYC | 1 cut(s) | 643 |
| BstC8I | GCNNGC | 6 cut(s) | 19, 166, 299, 537, 572, 712 |
| BstDEI | CTNAG | 4 cut(s) | 406, 488, 508, 658 |
| BstEII | GGTNACC | 1 cut(s) | 803 |
| BstF5I | GGATG | 3 cut(s) | 26, 34, 607 |
| BstFNI | CGCG | 1 cut(s) | 73 |
| BstH2I | RGCGCY | 1 cut(s) | 654 |
| BstHHI | GCGC | 4 cut(s) | 75, 214, 535, 653 |
| BstKTI | GATC | 4 cut(s) | 146, 179, 628, 773 |
| BstMAI | GTCTC | 1 cut(s) | 754 |
| BstMBI | GATC | 4 cut(s) | 143, 176, 625, 770 |
| BstMCI | CGRYCG | 1 cut(s) | 376 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 70, 127, 136, 303, 545, 567, 576 |
| BstNI | CCWGG | 3 cut(s) | 332, 367, 685 |
| BstPI | GGTNACC | 1 cut(s) | 803 |
| BstSCI | CCNGG | 6 cut(s) | 98, 330, 344, 365, 628, 683 |
| BstSFI | CTRYAG | 1 cut(s) | 708 |
| BstSLI | GKGCMC | 1 cut(s) | 691 |
| BstUI | CGCG | 1 cut(s) | 73 |
| BstV1I | GCAGC | 3 cut(s) | 133, 136, 686 |
| BstV2I | GAAGAC | 1 cut(s) | 285 |
| BstX2I | RGATCY | 2 cut(s) | 143, 176 |
| BstYI | RGATCY | 2 cut(s) | 143, 176 |
| BsuRI | GGCC | 5 cut(s) | 139, 291, 371, 539, 689 |
| BtgZI | GCGATG | 1 cut(s) | 589 |
| BtsCI | GGATG | 3 cut(s) | 26, 34, 607 |
| BtsIMutI | CAGTG | 2 cut(s) | 168, 393 |
| BveI | ACCTGC | 1 cut(s) | 377 |
| Cac8I | GCNNGC | 6 cut(s) | 19, 166, 299, 537, 572, 712 |
| CciI | TCATGA | 1 cut(s) | 253 |
| CfoI | GCGC | 4 cut(s) | 75, 214, 535, 653 |
| Cfr10I | RCCGGY | 3 cut(s) | 351, 371, 722 |
| Cfr13I | GGNCC | 5 cut(s) | 137, 369, 423, 687, 688 |
| CseI | GACGC | 3 cut(s) | 290, 392, 632 |
| Csp6I | GTAC | 4 cut(s) | 158, 363, 378, 390 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 391, 426 |
| CviAII | CATG | 2 cut(s) | 254, 785 |
| CviQI | GTAC | 4 cut(s) | 158, 363, 378, 390 |
| DdeI | CTNAG | 4 cut(s) | 406, 488, 508, 658 |
| DpnI | GATC | 4 cut(s) | 145, 178, 627, 772 |
| DpnII | GATC | 4 cut(s) | 143, 176, 625, 770 |
| DraIII | CACNNNGTG | 1 cut(s) | 312 |
| Eam1104I | CTCTTC | 2 cut(s) | 7, 675 |
| EarI | CTCTTC | 2 cut(s) | 7, 675 |
| EciI | GGCGGA | 3 cut(s) | 189, 648, 775 |
| Eco130I | CCWWGG | 1 cut(s) | 528 |
| Eco24I | GRGCYC | 1 cut(s) | 691 |
| Eco47I | GGWCC | 1 cut(s) | 423 |
| Eco57I | CTGAAG | 2 cut(s) | 31, 247 |
| Eco91I | GGTNACC | 1 cut(s) | 803 |
| EcoO109I | RGGNCCY | 1 cut(s) | 688 |
| EcoO65I | GGTNACC | 1 cut(s) | 803 |
| EcoRII | CCWGG | 3 cut(s) | 330, 365, 683 |
| EcoT14I | CCWWGG | 1 cut(s) | 528 |
| EcoT38I | GRGCYC | 1 cut(s) | 691 |
| ErhI | CCWWGG | 1 cut(s) | 528 |
| FaeI | CATG | 2 cut(s) | 257, 788 |
| FaiI | YATR | 9 cut(s) | 156, 184, 255, 325, 435, 728, 730, 786, 839 |
| FaqI | GGGAC | 2 cut(s) | 436, 630 |
| FatI | CATG | 2 cut(s) | 253, 784 |
| FauI | CCCGC | 4 cut(s) | 38, 306, 481, 541 |
| FauNDI | CATATG | 1 cut(s) | 728 |
| FblI | GTMKAC | 1 cut(s) | 341 |
| Fnu4HI | GCNGC | 4 cut(s) | 122, 125, 128, 700 |
| FokI | GGATG | 3 cut(s) | 13, 21, 614 |
| FriOI | GRGCYC | 1 cut(s) | 691 |
| Fsp4HI | GCNGC | 4 cut(s) | 122, 125, 128, 700 |
| FspBI | CTAG | 3 cut(s) | 165, 496, 544 |
| GlaI | GCGC | 4 cut(s) | 74, 213, 534, 652 |
| GluI | GCNGC | 4 cut(s) | 122, 125, 128, 700 |
| GsaI | CCCAGC | 3 cut(s) | 57, 306, 651 |
| GsuI | CTGGAG | 1 cut(s) | 306 |
| HaeII | RGCGCY | 1 cut(s) | 654 |
| HaeIII | GGCC | 5 cut(s) | 139, 291, 371, 539, 689 |
| HapII | CCGG | 6 cut(s) | 100, 346, 352, 372, 629, 723 |
| HgaI | GACGC | 3 cut(s) | 290, 392, 632 |
| HhaI | GCGC | 4 cut(s) | 75, 214, 535, 653 |
| Hin1I | GRCGYC | 1 cut(s) | 643 |
| Hin1II | CATG | 2 cut(s) | 257, 788 |
| Hin6I | GCGC | 4 cut(s) | 73, 212, 533, 651 |
| HinP1I | GCGC | 4 cut(s) | 73, 212, 533, 651 |
| HincII | GTYRAC | 1 cut(s) | 68 |
| HindII | GTYRAC | 1 cut(s) | 68 |
| HinfI | GANTC | 4 cut(s) | 465, 564, 636, 662 |
| HpaI | GTTAAC | 1 cut(s) | 68 |
| HpaII | CCGG | 6 cut(s) | 100, 346, 352, 372, 629, 723 |
| HphI | GGTGA | 3 cut(s) | 117, 686, 797 |
| Hpy166II | GTNNAC | 3 cut(s) | 68, 342, 782 |
| Hpy188I | TCNGA | 5 cut(s) | 50, 491, 661, 720, 770 |
| Hpy188III | TCNNGA | 3 cut(s) | 254, 679, 797 |
| Hpy8I | GTNNAC | 3 cut(s) | 68, 342, 782 |
| Hpy99I | CGWCG | 3 cut(s) | 137, 828, 831 |
| HpyAV | CCTTC | 3 cut(s) | 701, 794, 803 |
| HpyCH4IV | ACGT | 2 cut(s) | 200, 260 |
| HpyCH4V | TGCA | 5 cut(s) | 579, 605, 702, 710, 732 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 70, 127, 136, 303, 545, 567, 576 |
| HpyF3I | CTNAG | 4 cut(s) | 406, 488, 508, 658 |
| HpySE526I | ACGT | 2 cut(s) | 200, 260 |
| Hsp92I | GRCGYC | 1 cut(s) | 643 |
| Hsp92II | CATG | 2 cut(s) | 257, 788 |
| HspAI | GCGC | 4 cut(s) | 73, 212, 533, 651 |
| KpnI | GGTACC | 2 cut(s) | 366, 393 |
| KspAI | GTTAAC | 1 cut(s) | 68 |
| Kzo9I | GATC | 4 cut(s) | 143, 176, 625, 770 |
| LguI | GCTCTTC | 2 cut(s) | 7, 675 |
| LmnI | GCTCC | 2 cut(s) | 209, 614 |
| Lsp1109I | GCAGC | 3 cut(s) | 133, 136, 686 |
| LweI | GCATC | 2 cut(s) | 566, 592 |
| MaeI | CTAG | 3 cut(s) | 165, 496, 544 |
| MaeII | ACGT | 2 cut(s) | 200, 260 |
| MaeIII | GTNAC | 3 cut(s) | 149, 319, 803 |
| MalI | GATC | 4 cut(s) | 145, 178, 627, 772 |
| MbiI | CCGCTC | 2 cut(s) | 487, 668 |
| MboI | GATC | 4 cut(s) | 143, 176, 625, 770 |
| MboII | GAAGA | 4 cut(s) | 24, 171, 290, 662 |
| MfeI | CAATTG | 1 cut(s) | 609 |
| MflI | RGATCY | 2 cut(s) | 143, 176 |
| MhlI | GDGCHC | 1 cut(s) | 691 |
| MluCI | AATT | 1 cut(s) | 609 |
| MlyI | GAGTC | 1 cut(s) | 645 |
| MmeI | TCCRAC | 2 cut(s) | 73, 574 |
| MseI | TTAA | 1 cut(s) | 67 |
| MspA1I | CMGCKG | 3 cut(s) | 57, 570, 699 |
| MspI | CCGG | 6 cut(s) | 100, 346, 352, 372, 629, 723 |
| MspR9I | CCNGG | 6 cut(s) | 100, 332, 346, 367, 630, 685 |
| MunI | CAATTG | 1 cut(s) | 609 |
| MvaI | CCWGG | 3 cut(s) | 332, 367, 685 |
| MvnI | CGCG | 1 cut(s) | 73 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 70, 127, 136, 303, 545, 567, 576 |
| NciI | CCSGG | 3 cut(s) | 100, 346, 630 |
| NdeI | CATATG | 1 cut(s) | 728 |
| NdeII | GATC | 4 cut(s) | 143, 176, 625, 770 |
| NheI | GCTAGC | 1 cut(s) | 164 |
| NlaIII | CATG | 2 cut(s) | 257, 788 |
| NlaIV | GGNNCC | 5 cut(s) | 350, 364, 391, 424, 689 |
| NmuCI | GTSAC | 3 cut(s) | 149, 319, 803 |
| PagI | TCATGA | 1 cut(s) | 253 |
| PciSI | GCTCTTC | 2 cut(s) | 7, 675 |
| PfeI | GAWTC | 3 cut(s) | 465, 564, 662 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 593 |
| PkrI | GCNGC | 4 cut(s) | 123, 126, 129, 701 |
| PleI | GAGTC | 1 cut(s) | 644 |
| PpsI | GAGTC | 1 cut(s) | 644 |
| Psp6I | CCWGG | 3 cut(s) | 330, 365, 683 |
| PspEI | GGTNACC | 1 cut(s) | 803 |
| PspFI | CCCAGC | 3 cut(s) | 53, 302, 647 |
| PspGI | CCWGG | 3 cut(s) | 330, 365, 683 |
| PspN4I | GGNNCC | 5 cut(s) | 350, 364, 391, 424, 689 |
| PspOMI | GGGCCC | 1 cut(s) | 687 |
| PspPI | GGNCC | 5 cut(s) | 137, 369, 423, 687, 688 |
| PstI | CTGCAG | 1 cut(s) | 712 |
| PsuI | RGATCY | 2 cut(s) | 143, 176 |
| RsaI | GTAC | 4 cut(s) | 159, 364, 379, 391 |
| RsaNI | GTAC | 4 cut(s) | 158, 363, 378, 390 |
| SapI | GCTCTTC | 2 cut(s) | 7, 675 |
| SaqAI | TTAA | 1 cut(s) | 67 |
| SatI | GCNGC | 4 cut(s) | 122, 125, 128, 700 |
| Sau3AI | GATC | 4 cut(s) | 143, 176, 625, 770 |
| Sau96I | GGNCC | 5 cut(s) | 137, 369, 423, 687, 688 |
| SbfI | CCTGCAGG | 1 cut(s) | 712 |
| SchI | GAGTC | 1 cut(s) | 645 |
| ScrFI | CCNGG | 6 cut(s) | 100, 332, 346, 367, 630, 685 |
| SdaI | CCTGCAGG | 1 cut(s) | 712 |
| SduI | GDGCHC | 1 cut(s) | 691 |
| SfaNI | GCATC | 2 cut(s) | 566, 592 |
| SfcI | CTRYAG | 1 cut(s) | 708 |
| SinI | GGWCC | 1 cut(s) | 423 |
| Sse8387I | CCTGCAGG | 1 cut(s) | 712 |
| Sse9I | AATT | 1 cut(s) | 609 |
| SspMI | CTAG | 3 cut(s) | 165, 496, 544 |
| StyD4I | CCNGG | 6 cut(s) | 98, 330, 344, 365, 628, 683 |
| StyI | CCWWGG | 1 cut(s) | 528 |
| TaiI | ACGT | 2 cut(s) | 203, 263 |
| TaqI | TCGA | 1 cut(s) | 823 |
| TasI | AATT | 1 cut(s) | 609 |
| TauI | GCSGC | 1 cut(s) | 130 |
| TfiI | GAWTC | 3 cut(s) | 465, 564, 662 |
| Tru1I | TTAA | 1 cut(s) | 67 |
| Tru9I | TTAA | 1 cut(s) | 67 |
| TscAI | CASTG | 2 cut(s) | 175, 400 |
| TseFI | GTSAC | 3 cut(s) | 149, 319, 803 |
| TseI | GCWGC | 3 cut(s) | 121, 124, 699 |
| Tsp45I | GTSAC | 3 cut(s) | 149, 319, 803 |
| TspDTI | ATGAA | 1 cut(s) | 171 |
| TspRI | CASTG | 2 cut(s) | 175, 400 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 593 |
| VpaK11BI | GGWCC | 1 cut(s) | 423 |
| XmiI | GTMKAC | 1 cut(s) | 341 |
| XspI | CTAG | 3 cut(s) | 165, 496, 544 |