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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccII | CGCG | 1 cut(s) | 90 |
| AclI | AACGTT | 1 cut(s) | 299 |
| AclWI | GGATC | 1 cut(s) | 201 |
| AcoI | YGGCCR | 1 cut(s) | 261 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 77, 283 |
| AjnI | CCWGG | 2 cut(s) | 133, 151 |
| AluBI | AGCT | 1 cut(s) | 289 |
| AluI | AGCT | 1 cut(s) | 289 |
| AlwI | GGATC | 1 cut(s) | 201 |
| AoxI | GGCC | 3 cut(s) | 154, 246, 261 |
| ApeKI | GCWGC | 2 cut(s) | 141, 253 |
| AspLEI | GCGC | 1 cut(s) | 92 |
| AspS9I | GGNCC | 1 cut(s) | 247 |
| AsuC2I | CCSGG | 2 cut(s) | 107, 320 |
| AsuHPI | GGTGA | 2 cut(s) | 91, 134 |
| BalI | TGGCCA | 1 cut(s) | 263 |
| BbsI | GAAGAC | 1 cut(s) | 27 |
| BbvI | GCAGC | 2 cut(s) | 153, 265 |
| BciT130I | CCWGG | 2 cut(s) | 135, 153 |
| BcnI | CCSGG | 2 cut(s) | 107, 320 |
| BfaI | CTAG | 1 cut(s) | 6 |
| BglI | GCCNNNNNGGC | 2 cut(s) | 153, 320 |
| BisI | GCNGC | 3 cut(s) | 142, 145, 254 |
| BlsI | GCNGC | 3 cut(s) | 143, 146, 255 |
| Bme1390I | CCNGG | 4 cut(s) | 107, 135, 153, 320 |
| BmgT120I | GGNCC | 1 cut(s) | 247 |
| BmrFI | CCNGG | 4 cut(s) | 107, 135, 153, 320 |
| BmsI | GCATC | 1 cut(s) | 90 |
| BpiI | GAAGAC | 1 cut(s) | 27 |
| BpuMI | CCSGG | 2 cut(s) | 107, 320 |
| BsaJI | CCNNGG | 3 cut(s) | 105, 133, 243 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 77, 283 |
| Bse1I | ACTGG | 3 cut(s) | 43, 186, 192 |
| BseBI | CCWGG | 2 cut(s) | 135, 153 |
| BseDI | CCNNGG | 3 cut(s) | 105, 133, 243 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 77, 283 |
| BseNI | ACTGG | 3 cut(s) | 43, 186, 192 |
| BseRI | GAGGAG | 1 cut(s) | 299 |
| BseXI | GCAGC | 2 cut(s) | 153, 265 |
| BseYI | CCCAGC | 3 cut(s) | 70, 116, 249 |
| Bsh1236I | CGCG | 1 cut(s) | 90 |
| BshFI | GGCC | 3 cut(s) | 156, 248, 263 |
| BsiSI | CCGG | 2 cut(s) | 107, 320 |
| BslFI | GGGAC | 1 cut(s) | 259 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 77, 283 |
| BsmFI | GGGAC | 1 cut(s) | 259 |
| BsnI | GGCC | 3 cut(s) | 156, 248, 263 |
| Bsp143I | GATC | 1 cut(s) | 193 |
| BspANI | GGCC | 3 cut(s) | 156, 248, 263 |
| BspFNI | CGCG | 1 cut(s) | 90 |
| BspPI | GGATC | 1 cut(s) | 201 |
| BsrI | ACTGG | 3 cut(s) | 43, 186, 192 |
| BssECI | CCNNGG | 3 cut(s) | 105, 133, 243 |
| BssMI | GATC | 1 cut(s) | 193 |
| Bst2UI | CCWGG | 2 cut(s) | 135, 153 |
| BstC8I | GCNNGC | 2 cut(s) | 36, 149 |
| BstEII | GGTNACC | 1 cut(s) | 307 |
| BstFNI | CGCG | 1 cut(s) | 90 |
| BstHHI | GCGC | 1 cut(s) | 92 |
| BstKTI | GATC | 1 cut(s) | 196 |
| BstMBI | GATC | 1 cut(s) | 193 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 87, 153, 320 |
| BstNI | CCWGG | 2 cut(s) | 135, 153 |
| BstPI | GGTNACC | 1 cut(s) | 307 |
| BstSCI | CCNGG | 4 cut(s) | 105, 133, 151, 318 |
| BstUI | CGCG | 1 cut(s) | 90 |
| BstV1I | GCAGC | 2 cut(s) | 153, 265 |
| BstV2I | GAAGAC | 1 cut(s) | 27 |
| BstX2I | RGATCY | 1 cut(s) | 193 |
| BstYI | RGATCY | 1 cut(s) | 193 |
| BsuRI | GGCC | 3 cut(s) | 156, 248, 263 |
| BtsIMutI | CAGTG | 1 cut(s) | 185 |
| Cac8I | GCNNGC | 2 cut(s) | 36, 149 |
| CfoI | GCGC | 1 cut(s) | 92 |
| Cfr13I | GGNCC | 1 cut(s) | 247 |
| CviAII | CATG | 3 cut(s) | 224, 265, 324 |
| CviJI | RGCY | 6 cut(s) | 144, 156, 242, 248, 263, 289 |
| CviKI_1 | RGCY | 6 cut(s) | 144, 156, 242, 248, 263, 289 |
| DpnI | GATC | 1 cut(s) | 195 |
| DpnII | GATC | 1 cut(s) | 193 |
| EaeI | YGGCCR | 1 cut(s) | 261 |
| EciI | GGCGGA | 2 cut(s) | 37, 206 |
| Eco91I | GGTNACC | 1 cut(s) | 307 |
| EcoO65I | GGTNACC | 1 cut(s) | 307 |
| EcoRII | CCWGG | 2 cut(s) | 133, 151 |
| FaeI | CATG | 3 cut(s) | 227, 268, 327 |
| FaiI | YATR | 4 cut(s) | 177, 225, 266, 325 |
| FaqI | GGGAC | 1 cut(s) | 259 |
| FatI | CATG | 3 cut(s) | 223, 264, 323 |
| FauI | CCCGC | 2 cut(s) | 156, 284 |
| Fnu4HI | GCNGC | 3 cut(s) | 142, 145, 254 |
| Fsp4HI | GCNGC | 3 cut(s) | 142, 145, 254 |
| FspBI | CTAG | 1 cut(s) | 6 |
| GlaI | GCGC | 1 cut(s) | 91 |
| GluI | GCNGC | 3 cut(s) | 142, 145, 254 |
| GsaI | CCCAGC | 3 cut(s) | 74, 120, 253 |
| HaeIII | GGCC | 3 cut(s) | 156, 248, 263 |
| HapII | CCGG | 2 cut(s) | 107, 320 |
| HhaI | GCGC | 1 cut(s) | 92 |
| Hin1II | CATG | 3 cut(s) | 227, 268, 327 |
| Hin6I | GCGC | 1 cut(s) | 90 |
| HinP1I | GCGC | 1 cut(s) | 90 |
| HincII | GTYRAC | 2 cut(s) | 28, 112 |
| HindII | GTYRAC | 2 cut(s) | 28, 112 |
| HpaII | CCGG | 2 cut(s) | 107, 320 |
| HphI | GGTGA | 2 cut(s) | 91, 134 |
| Hpy166II | GTNNAC | 3 cut(s) | 28, 112, 221 |
| Hpy8I | GTNNAC | 3 cut(s) | 28, 112, 221 |
| HpyCH4IV | ACGT | 3 cut(s) | 24, 217, 299 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 87, 153, 320 |
| HpySE526I | ACGT | 3 cut(s) | 24, 217, 299 |
| Hsp92II | CATG | 3 cut(s) | 227, 268, 327 |
| HspAI | GCGC | 1 cut(s) | 90 |
| Kzo9I | GATC | 1 cut(s) | 193 |
| LmnI | GCTCC | 1 cut(s) | 286 |
| Lsp1109I | GCAGC | 2 cut(s) | 153, 265 |
| LweI | GCATC | 1 cut(s) | 90 |
| MaeI | CTAG | 1 cut(s) | 6 |
| MaeII | ACGT | 3 cut(s) | 24, 217, 299 |
| MaeIII | GTNAC | 1 cut(s) | 307 |
| MalI | GATC | 1 cut(s) | 195 |
| MboI | GATC | 1 cut(s) | 193 |
| MboII | GAAGA | 2 cut(s) | 32, 188 |
| MflI | RGATCY | 1 cut(s) | 193 |
| MlsI | TGGCCA | 1 cut(s) | 263 |
| MluNI | TGGCCA | 1 cut(s) | 263 |
| MmeI | TCCRAC | 1 cut(s) | 282 |
| MnlI | CCTC | 6 cut(s) | 118, 173, 178, 238, 277, 298 |
| Mox20I | TGGCCA | 1 cut(s) | 263 |
| MscI | TGGCCA | 1 cut(s) | 263 |
| MslI | CAYNNNNRTG | 1 cut(s) | 269 |
| Msp20I | TGGCCA | 1 cut(s) | 263 |
| MspA1I | CMGCKG | 2 cut(s) | 74, 120 |
| MspI | CCGG | 2 cut(s) | 107, 320 |
| MspR9I | CCNGG | 4 cut(s) | 107, 135, 153, 320 |
| MvaI | CCWGG | 2 cut(s) | 135, 153 |
| MvnI | CGCG | 1 cut(s) | 90 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 87, 153, 320 |
| NciI | CCSGG | 2 cut(s) | 107, 320 |
| NdeII | GATC | 1 cut(s) | 193 |
| NlaIII | CATG | 3 cut(s) | 227, 268, 327 |
| NmeAIII | GCCGAG | 1 cut(s) | 268 |
| PkrI | GCNGC | 3 cut(s) | 143, 146, 255 |
| Psp1406I | AACGTT | 1 cut(s) | 299 |
| Psp6I | CCWGG | 2 cut(s) | 133, 151 |
| PspEI | GGTNACC | 1 cut(s) | 307 |
| PspFI | CCCAGC | 3 cut(s) | 70, 116, 249 |
| PspGI | CCWGG | 2 cut(s) | 133, 151 |
| PspPI | GGNCC | 1 cut(s) | 247 |
| PsuI | RGATCY | 1 cut(s) | 193 |
| RseI | CAYNNNNRTG | 1 cut(s) | 269 |
| SatI | GCNGC | 3 cut(s) | 142, 145, 254 |
| Sau3AI | GATC | 1 cut(s) | 193 |
| Sau96I | GGNCC | 1 cut(s) | 247 |
| ScrFI | CCNGG | 4 cut(s) | 107, 135, 153, 320 |
| SetI | ASST | 7 cut(s) | 27, 139, 165, 220, 291, 302, 309 |
| SfaNI | GCATC | 1 cut(s) | 90 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 269 |
| SspMI | CTAG | 1 cut(s) | 6 |
| StyD4I | CCNGG | 4 cut(s) | 105, 133, 151, 318 |
| TaiI | ACGT | 3 cut(s) | 27, 220, 302 |
| TauI | GCSGC | 1 cut(s) | 147 |
| TscAI | CASTG | 1 cut(s) | 192 |
| TseI | GCWGC | 2 cut(s) | 141, 253 |
| TspDTI | ATGAA | 2 cut(s) | 192, 240 |
| TspRI | CASTG | 1 cut(s) | 192 |
| XspI | CTAG | 1 cut(s) | 6 |