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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 105, 254 |
| Acc65I | GGTACC | 1 cut(s) | 117 |
| AccB1I | GGYRCC | 1 cut(s) | 117 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 321 |
| AciI | CCGC | 5 cut(s) | 43, 157, 202, 247, 276 |
| AclWI | GGATC | 1 cut(s) | 53 |
| AcuI | CTGAAG | 1 cut(s) | 257 |
| AfaI | GTAC | 2 cut(s) | 28, 119 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 219, 321 |
| AjnI | CCWGG | 2 cut(s) | 271, 284 |
| AluBI | AGCT | 1 cut(s) | 33 |
| AluI | AGCT | 1 cut(s) | 33 |
| AlwI | GGATC | 1 cut(s) | 53 |
| AoxI | GGCC | 2 cut(s) | 6, 98 |
| Asp718I | GGTACC | 1 cut(s) | 117 |
| AspLEI | GCGC | 1 cut(s) | 263 |
| AspS9I | GGNCC | 3 cut(s) | 6, 99, 151 |
| AsuC2I | CCSGG | 1 cut(s) | 5 |
| AsuNHI | GCTAGC | 1 cut(s) | 33 |
| AvaII | GGWCC | 1 cut(s) | 151 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 101, 134 |
| BanI | GGYRCC | 1 cut(s) | 117 |
| BbvCI | CCTCAGC | 1 cut(s) | 95 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 292, 326 |
| BciT130I | CCWGG | 2 cut(s) | 273, 286 |
| BcnI | CCSGG | 1 cut(s) | 5 |
| BfaI | CTAG | 1 cut(s) | 34 |
| BfuAI | ACCTGC | 2 cut(s) | 105, 254 |
| BglII | AGATCT | 1 cut(s) | 12 |
| Bme1390I | CCNGG | 3 cut(s) | 5, 273, 286 |
| Bme18I | GGWCC | 1 cut(s) | 151 |
| BmgT120I | GGNCC | 3 cut(s) | 6, 99, 151 |
| BmiI | GGNNCC | 2 cut(s) | 119, 152 |
| BmrFI | CCNGG | 3 cut(s) | 5, 273, 286 |
| BmsI | GCATC | 1 cut(s) | 294 |
| BmtI | GCTAGC | 1 cut(s) | 37 |
| Bpu10I | CCTNAGC | 1 cut(s) | 95 |
| BpuMI | CCSGG | 1 cut(s) | 5 |
| BsaJI | CCNNGG | 1 cut(s) | 256 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 219, 321 |
| Bse1I | ACTGG | 2 cut(s) | 44, 344 |
| BseBI | CCWGG | 2 cut(s) | 273, 286 |
| BseDI | CCNNGG | 1 cut(s) | 256 |
| BseGI | GGATG | 1 cut(s) | 281 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 219, 321 |
| BseMII | CTCAG | 2 cut(s) | 86, 125 |
| BseNI | ACTGG | 2 cut(s) | 44, 344 |
| BseYI | CCCAGC | 1 cut(s) | 141 |
| BshFI | GGCC | 2 cut(s) | 8, 100 |
| BshNI | GGYRCC | 1 cut(s) | 117 |
| BsiSI | CCGG | 1 cut(s) | 4 |
| BslFI | GGGAC | 1 cut(s) | 164 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 219, 321 |
| BsmFI | GGGAC | 1 cut(s) | 164 |
| BsnI | GGCC | 2 cut(s) | 8, 100 |
| Bsp143I | GATC | 2 cut(s) | 12, 45 |
| BspACI | CCGC | 5 cut(s) | 43, 157, 202, 247, 276 |
| BspANI | GGCC | 2 cut(s) | 8, 100 |
| BspCNI | CTCAG | 2 cut(s) | 87, 126 |
| BspLI | GGNNCC | 2 cut(s) | 119, 152 |
| BspMI | ACCTGC | 2 cut(s) | 105, 254 |
| BspOI | GCTAGC | 1 cut(s) | 37 |
| BspPI | GGATC | 1 cut(s) | 53 |
| BspT107I | GGYRCC | 1 cut(s) | 117 |
| BsrI | ACTGG | 2 cut(s) | 44, 344 |
| BssECI | CCNNGG | 1 cut(s) | 256 |
| BssMI | GATC | 2 cut(s) | 12, 45 |
| BssT1I | CCWWGG | 1 cut(s) | 256 |
| Bst2UI | CCWGG | 2 cut(s) | 273, 286 |
| Bst4CI | ACNGT | 1 cut(s) | 214 |
| BstC8I | GCNNGC | 1 cut(s) | 35 |
| BstDEI | CTNAG | 2 cut(s) | 95, 134 |
| BstF5I | GGATG | 1 cut(s) | 281 |
| BstHHI | GCGC | 1 cut(s) | 263 |
| BstKTI | GATC | 2 cut(s) | 15, 48 |
| BstMBI | GATC | 2 cut(s) | 12, 45 |
| BstNI | CCWGG | 2 cut(s) | 273, 286 |
| BstSCI | CCNGG | 3 cut(s) | 3, 271, 284 |
| BstX2I | RGATCY | 2 cut(s) | 12, 45 |
| BstYI | RGATCY | 2 cut(s) | 12, 45 |
| BsuRI | GGCC | 2 cut(s) | 8, 100 |
| BtgZI | GCGATG | 1 cut(s) | 317 |
| BtsCI | GGATG | 1 cut(s) | 281 |
| BtsIMutI | CAGTG | 2 cut(s) | 37, 121 |
| BveI | ACCTGC | 2 cut(s) | 105, 254 |
| Cac8I | GCNNGC | 1 cut(s) | 35 |
| CfoI | GCGC | 1 cut(s) | 263 |
| Cfr13I | GGNCC | 3 cut(s) | 6, 99, 151 |
| CseI | GACGC | 1 cut(s) | 120 |
| Csp6I | GTAC | 2 cut(s) | 27, 118 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 119, 154 |
| CviJI | RGCY | 5 cut(s) | 8, 33, 94, 100, 289 |
| CviKI_1 | RGCY | 5 cut(s) | 8, 33, 94, 100, 289 |
| CviQI | GTAC | 2 cut(s) | 27, 118 |
| DdeI | CTNAG | 2 cut(s) | 95, 134 |
| DpnI | GATC | 2 cut(s) | 14, 47 |
| DpnII | GATC | 2 cut(s) | 12, 45 |
| EciI | GGCGGA | 2 cut(s) | 58, 236 |
| Eco130I | CCWWGG | 1 cut(s) | 256 |
| Eco47I | GGWCC | 1 cut(s) | 151 |
| Eco57I | CTGAAG | 1 cut(s) | 257 |
| EcoRII | CCWGG | 2 cut(s) | 271, 284 |
| EcoT14I | CCWWGG | 1 cut(s) | 256 |
| ErhI | CCWWGG | 1 cut(s) | 256 |
| FaiI | YATR | 4 cut(s) | 25, 53, 83, 163 |
| FaqI | GGGAC | 1 cut(s) | 164 |
| FauI | CCCGC | 2 cut(s) | 209, 269 |
| FokI | GGATG | 1 cut(s) | 268 |
| FspBI | CTAG | 1 cut(s) | 34 |
| GlaI | GCGC | 1 cut(s) | 262 |
| GsaI | CCCAGC | 1 cut(s) | 145 |
| HaeIII | GGCC | 2 cut(s) | 8, 100 |
| HapII | CCGG | 1 cut(s) | 4 |
| HgaI | GACGC | 1 cut(s) | 120 |
| HhaI | GCGC | 1 cut(s) | 263 |
| Hin6I | GCGC | 1 cut(s) | 261 |
| HinP1I | GCGC | 1 cut(s) | 261 |
| HinfI | GANTC | 2 cut(s) | 193, 292 |
| HpaII | CCGG | 1 cut(s) | 4 |
| Hpy188I | TCNGA | 1 cut(s) | 219 |
| Hpy99I | CGWCG | 1 cut(s) | 301 |
| HpyAV | CCTTC | 3 cut(s) | 69, 140, 232 |
| HpyCH4III | ACNGT | 1 cut(s) | 214 |
| HpyCH4IV | ACGT | 1 cut(s) | 69 |
| HpyCH4V | TGCA | 2 cut(s) | 307, 333 |
| HpyF3I | CTNAG | 2 cut(s) | 95, 134 |
| HpySE526I | ACGT | 1 cut(s) | 69 |
| HspAI | GCGC | 1 cut(s) | 261 |
| KpnI | GGTACC | 1 cut(s) | 121 |
| Kzo9I | GATC | 2 cut(s) | 12, 45 |
| LmnI | GCTCC | 1 cut(s) | 78 |
| LweI | GCATC | 1 cut(s) | 294 |
| MaeI | CTAG | 1 cut(s) | 34 |
| MaeII | ACGT | 1 cut(s) | 69 |
| MaeIII | GTNAC | 1 cut(s) | 18 |
| MalI | GATC | 2 cut(s) | 14, 47 |
| MboI | GATC | 2 cut(s) | 12, 45 |
| MboII | GAAGA | 1 cut(s) | 40 |
| MflI | RGATCY | 2 cut(s) | 12, 45 |
| MmeI | TCCRAC | 1 cut(s) | 302 |
| MnlI | CCTC | 4 cut(s) | 90, 180, 213, 224 |
| MspI | CCGG | 1 cut(s) | 4 |
| MspR9I | CCNGG | 3 cut(s) | 5, 273, 286 |
| MvaI | CCWGG | 2 cut(s) | 273, 286 |
| NciI | CCSGG | 1 cut(s) | 5 |
| NdeII | GATC | 2 cut(s) | 12, 45 |
| NheI | GCTAGC | 1 cut(s) | 33 |
| NlaIV | GGNNCC | 2 cut(s) | 119, 152 |
| NmuCI | GTSAC | 1 cut(s) | 18 |
| PfeI | GAWTC | 2 cut(s) | 193, 292 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 321 |
| Psp6I | CCWGG | 2 cut(s) | 271, 284 |
| PspFI | CCCAGC | 1 cut(s) | 141 |
| PspGI | CCWGG | 2 cut(s) | 271, 284 |
| PspN4I | GGNNCC | 2 cut(s) | 119, 152 |
| PspPI | GGNCC | 3 cut(s) | 6, 99, 151 |
| PsuI | RGATCY | 2 cut(s) | 12, 45 |
| RsaI | GTAC | 2 cut(s) | 28, 119 |
| RsaNI | GTAC | 2 cut(s) | 27, 118 |
| Sau3AI | GATC | 2 cut(s) | 12, 45 |
| Sau96I | GGNCC | 3 cut(s) | 6, 99, 151 |
| ScrFI | CCNGG | 3 cut(s) | 5, 273, 286 |
| SetI | ASST | 5 cut(s) | 35, 72, 119, 135, 268 |
| SfaNI | GCATC | 1 cut(s) | 294 |
| SinI | GGWCC | 1 cut(s) | 151 |
| SsiI | CCGC | 5 cut(s) | 43, 157, 202, 247, 276 |
| SspMI | CTAG | 1 cut(s) | 34 |
| StyD4I | CCNGG | 3 cut(s) | 3, 271, 284 |
| StyI | CCWWGG | 1 cut(s) | 256 |
| TaaI | ACNGT | 1 cut(s) | 214 |
| TaiI | ACGT | 1 cut(s) | 72 |
| TfiI | GAWTC | 2 cut(s) | 193, 292 |
| TscAI | CASTG | 2 cut(s) | 44, 128 |
| TseFI | GTSAC | 1 cut(s) | 18 |
| Tsp45I | GTSAC | 1 cut(s) | 18 |
| TspDTI | ATGAA | 1 cut(s) | 40 |
| TspGWI | ACGGA | 1 cut(s) | 285 |
| TspRI | CASTG | 2 cut(s) | 44, 128 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 321 |
| VpaK11BI | GGWCC | 1 cut(s) | 151 |
| XspI | CTAG | 1 cut(s) | 34 |