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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 1 cut(s) | 352 |
| Acc65I | GGTACC | 1 cut(s) | 364 |
| AccB1I | GGYRCC | 1 cut(s) | 364 |
| AccBSI | CCGCTC | 1 cut(s) | 462 |
| AciI | CCGC | 9 cut(s) | 15, 38, 74, 92, 135, 368, 415, 449, 460 |
| AclWI | GGATC | 2 cut(s) | 48, 135 |
| AcoI | YGGCCR | 2 cut(s) | 75, 228 |
| AfaI | GTAC | 2 cut(s) | 339, 366 |
| AfiI | CCNNNNNNNGG | 2 cut(s) | 169, 424 |
| AgsI | TTSAA | 1 cut(s) | 118 |
| AluBI | AGCT | 3 cut(s) | 148, 268, 470 |
| AluI | AGCT | 3 cut(s) | 148, 268, 470 |
| Alw21I | GWGCWC | 2 cut(s) | 198, 314 |
| AlwI | GGATC | 2 cut(s) | 48, 135 |
| AoxI | GGCC | 4 cut(s) | 75, 207, 228, 397 |
| ApaI | GGGCCC | 1 cut(s) | 401 |
| ApeKI | GCWGC | 1 cut(s) | 467 |
| AseI | ATTAAT | 1 cut(s) | 236 |
| Asp718I | GGTACC | 1 cut(s) | 364 |
| AspS9I | GGNCC | 3 cut(s) | 160, 397, 398 |
| AvaII | GGWCC | 1 cut(s) | 160 |
| BaeGI | GKGCMC | 1 cut(s) | 401 |
| BalI | TGGCCA | 1 cut(s) | 230 |
| BanI | GGYRCC | 1 cut(s) | 364 |
| BanII | GRGCYC | 1 cut(s) | 401 |
| Bbv12I | GWGCWC | 2 cut(s) | 198, 314 |
| BbvCI | CCTCAGC | 1 cut(s) | 27 |
| BbvI | GCAGC | 1 cut(s) | 479 |
| BccI | CCATC | 1 cut(s) | 418 |
| BfaI | CTAG | 2 cut(s) | 471, 477 |
| BfuAI | ACCTGC | 1 cut(s) | 352 |
| BisI | GCNGC | 4 cut(s) | 75, 135, 449, 468 |
| BlsI | GCNGC | 4 cut(s) | 76, 136, 450, 469 |
| Bme18I | GGWCC | 1 cut(s) | 160 |
| BmgT120I | GGNCC | 3 cut(s) | 160, 397, 398 |
| BmiI | GGNNCC | 2 cut(s) | 366, 399 |
| BmsI | GCATC | 1 cut(s) | 348 |
| BpmI | CTGGAG | 1 cut(s) | 290 |
| Bpu10I | CCTNAGC | 1 cut(s) | 27 |
| Bsc4I | CCNNNNNNNGG | 2 cut(s) | 169, 424 |
| BseLI | CCNNNNNNNGG | 2 cut(s) | 169, 424 |
| BseMII | CTCAG | 1 cut(s) | 18 |
| BseRI | GAGGAG | 1 cut(s) | 44 |
| BseSI | GKGCMC | 1 cut(s) | 401 |
| BseXI | GCAGC | 1 cut(s) | 479 |
| BseYI | CCCAGC | 3 cut(s) | 70, 88, 286 |
| BshFI | GGCC | 4 cut(s) | 77, 209, 230, 399 |
| BshNI | GGYRCC | 1 cut(s) | 364 |
| BsiHKAI | GWGCWC | 2 cut(s) | 198, 314 |
| BslI | CCNNNNNNNGG | 2 cut(s) | 169, 424 |
| BsnI | GGCC | 4 cut(s) | 77, 209, 230, 399 |
| Bsp120I | GGGCCC | 1 cut(s) | 397 |
| Bsp1286I | GDGCHC | 3 cut(s) | 198, 314, 401 |
| Bsp143I | GATC | 2 cut(s) | 40, 127 |
| BspACI | CCGC | 9 cut(s) | 15, 38, 74, 92, 135, 368, 415, 449, 460 |
| BspANI | GGCC | 4 cut(s) | 77, 209, 230, 399 |
| BspCNI | CTCAG | 1 cut(s) | 19 |
| BspLI | GGNNCC | 2 cut(s) | 366, 399 |
| BspMI | ACCTGC | 1 cut(s) | 352 |
| BspPI | GGATC | 2 cut(s) | 48, 135 |
| BspT107I | GGYRCC | 1 cut(s) | 364 |
| BsrBI | CCGCTC | 1 cut(s) | 462 |
| BssMI | GATC | 2 cut(s) | 40, 127 |
| Bst4CI | ACNGT | 2 cut(s) | 107, 337 |
| BstC8I | GCNNGC | 5 cut(s) | 36, 79, 150, 188, 299 |
| BstDEI | CTNAG | 1 cut(s) | 27 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 167 |
| BstKTI | GATC | 2 cut(s) | 43, 130 |
| BstMBI | GATC | 2 cut(s) | 40, 127 |
| BstMWI | GCNNNNNNNGC | 3 cut(s) | 287, 367, 473 |
| BstSLI | GKGCMC | 1 cut(s) | 401 |
| BstV1I | GCAGC | 1 cut(s) | 479 |
| BstX2I | RGATCY | 1 cut(s) | 127 |
| BstYI | RGATCY | 1 cut(s) | 127 |
| BsuRI | GGCC | 4 cut(s) | 77, 209, 230, 399 |
| BveI | ACCTGC | 1 cut(s) | 352 |
| Cac8I | GCNNGC | 5 cut(s) | 36, 79, 150, 188, 299 |
| Cfr13I | GGNCC | 3 cut(s) | 160, 397, 398 |
| Csp6I | GTAC | 2 cut(s) | 338, 365 |
| CviQI | GTAC | 2 cut(s) | 338, 365 |
| DdeI | CTNAG | 1 cut(s) | 27 |
| DpnI | GATC | 2 cut(s) | 42, 129 |
| DpnII | GATC | 2 cut(s) | 40, 127 |
| EaeI | YGGCCR | 2 cut(s) | 75, 228 |
| Eco24I | GRGCYC | 1 cut(s) | 401 |
| Eco47I | GGWCC | 1 cut(s) | 160 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 167 |
| EcoO109I | RGGNCCY | 1 cut(s) | 160 |
| EcoT38I | GRGCYC | 1 cut(s) | 401 |
| FaiI | YATR | 3 cut(s) | 140, 144, 353 |
| FauI | CCCGC | 2 cut(s) | 8, 422 |
| Fnu4HI | GCNGC | 4 cut(s) | 75, 135, 449, 468 |
| FriOI | GRGCYC | 1 cut(s) | 401 |
| Fsp4HI | GCNGC | 4 cut(s) | 75, 135, 449, 468 |
| FspBI | CTAG | 2 cut(s) | 471, 477 |
| GluI | GCNGC | 4 cut(s) | 75, 135, 449, 468 |
| GsaI | CCCAGC | 3 cut(s) | 74, 92, 290 |
| GsuI | CTGGAG | 1 cut(s) | 290 |
| HaeIII | GGCC | 4 cut(s) | 77, 209, 230, 399 |
| HincII | GTYRAC | 1 cut(s) | 103 |
| HindII | GTYRAC | 1 cut(s) | 103 |
| HpaI | GTTAAC | 1 cut(s) | 103 |
| Hpy166II | GTNNAC | 2 cut(s) | 103, 326 |
| Hpy188III | TCNNGA | 1 cut(s) | 125 |
| Hpy8I | GTNNAC | 2 cut(s) | 103, 326 |
| HpyAV | CCTTC | 1 cut(s) | 199 |
| HpyCH4III | ACNGT | 2 cut(s) | 107, 337 |
| HpyCH4IV | ACGT | 1 cut(s) | 319 |
| HpyCH4V | TGCA | 2 cut(s) | 110, 361 |
| HpyF10VI | GCNNNNNNNGC | 3 cut(s) | 287, 367, 473 |
| HpyF3I | CTNAG | 1 cut(s) | 27 |
| HpySE526I | ACGT | 1 cut(s) | 319 |
| KpnI | GGTACC | 1 cut(s) | 368 |
| KspAI | GTTAAC | 1 cut(s) | 103 |
| Kzo9I | GATC | 2 cut(s) | 40, 127 |
| LmnI | GCTCC | 3 cut(s) | 31, 265, 309 |
| Lsp1109I | GCAGC | 1 cut(s) | 479 |
| LweI | GCATC | 1 cut(s) | 348 |
| MaeI | CTAG | 2 cut(s) | 471, 477 |
| MaeII | ACGT | 1 cut(s) | 319 |
| MaeIII | GTNAC | 1 cut(s) | 441 |
| MalI | GATC | 2 cut(s) | 42, 129 |
| MbiI | CCGCTC | 1 cut(s) | 462 |
| MboI | GATC | 2 cut(s) | 40, 127 |
| MflI | RGATCY | 1 cut(s) | 127 |
| MhlI | GDGCHC | 3 cut(s) | 198, 314, 401 |
| MlsI | TGGCCA | 1 cut(s) | 230 |
| MluCI | AATT | 1 cut(s) | 245 |
| MluNI | TGGCCA | 1 cut(s) | 230 |
| MnlI | CCTC | 6 cut(s) | 22, 151, 173, 175, 265, 414 |
| Mox20I | TGGCCA | 1 cut(s) | 230 |
| MscI | TGGCCA | 1 cut(s) | 230 |
| MseI | TTAA | 4 cut(s) | 84, 96, 102, 236 |
| MslI | CAYNNNNRTG | 2 cut(s) | 236, 356 |
| Msp20I | TGGCCA | 1 cut(s) | 230 |
| MspA1I | CMGCKG | 3 cut(s) | 74, 92, 370 |
| MwoI | GCNNNNNNNGC | 3 cut(s) | 287, 367, 473 |
| NdeII | GATC | 2 cut(s) | 40, 127 |
| NlaIV | GGNNCC | 2 cut(s) | 366, 399 |
| NmeAIII | GCCGAG | 1 cut(s) | 443 |
| NmuCI | GTSAC | 1 cut(s) | 441 |
| PkrI | GCNGC | 4 cut(s) | 76, 136, 450, 469 |
| PpuMI | RGGWCCY | 1 cut(s) | 160 |
| PshBI | ATTAAT | 1 cut(s) | 236 |
| Psp5II | RGGWCCY | 1 cut(s) | 160 |
| PspFI | CCCAGC | 3 cut(s) | 70, 88, 286 |
| PspN4I | GGNNCC | 2 cut(s) | 366, 399 |
| PspOMI | GGGCCC | 1 cut(s) | 397 |
| PspPI | GGNCC | 3 cut(s) | 160, 397, 398 |
| PspPPI | RGGWCCY | 1 cut(s) | 160 |
| PsuI | RGATCY | 1 cut(s) | 127 |
| RsaI | GTAC | 2 cut(s) | 339, 366 |
| RsaNI | GTAC | 2 cut(s) | 338, 365 |
| RseI | CAYNNNNRTG | 2 cut(s) | 236, 356 |
| SaqAI | TTAA | 4 cut(s) | 84, 96, 102, 236 |
| SatI | GCNGC | 4 cut(s) | 75, 135, 449, 468 |
| Sau3AI | GATC | 2 cut(s) | 40, 127 |
| Sau96I | GGNCC | 3 cut(s) | 160, 397, 398 |
| SduI | GDGCHC | 3 cut(s) | 198, 314, 401 |
| SetI | ASST | 7 cut(s) | 150, 165, 270, 322, 366, 406, 472 |
| SfaNI | GCATC | 1 cut(s) | 348 |
| SinI | GGWCC | 1 cut(s) | 160 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 236, 356 |
| Sse9I | AATT | 1 cut(s) | 245 |
| SsiI | CCGC | 9 cut(s) | 15, 38, 74, 92, 135, 368, 415, 449, 460 |
| SspMI | CTAG | 2 cut(s) | 471, 477 |
| TaaI | ACNGT | 2 cut(s) | 107, 337 |
| TaiI | ACGT | 1 cut(s) | 322 |
| TasI | AATT | 1 cut(s) | 245 |
| TauI | GCSGC | 3 cut(s) | 77, 137, 451 |
| Tru1I | TTAA | 4 cut(s) | 84, 96, 102, 236 |
| Tru9I | TTAA | 4 cut(s) | 84, 96, 102, 236 |
| TseFI | GTSAC | 1 cut(s) | 441 |
| TseI | GCWGC | 1 cut(s) | 467 |
| Tsp45I | GTSAC | 1 cut(s) | 441 |
| TspGWI | ACGGA | 1 cut(s) | 322 |
| VpaK11BI | GGWCC | 1 cut(s) | 160 |
| VspI | ATTAAT | 1 cut(s) | 236 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 167 |
| XspI | CTAG | 2 cut(s) | 471, 477 |