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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 2 cut(s) | 43, 47 |
| AccII | CGCG | 1 cut(s) | 219 |
| AciI | CCGC | 4 cut(s) | 74, 120, 145, 149 |
| AcuI | CTGAAG | 1 cut(s) | 48 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 73 |
| AjnI | CCWGG | 1 cut(s) | 151 |
| AluBI | AGCT | 2 cut(s) | 181, 196 |
| AluI | AGCT | 2 cut(s) | 181, 196 |
| Alw21I | GWGCWC | 1 cut(s) | 94 |
| Alw26I | GTCTC | 1 cut(s) | 119 |
| Alw44I | GTGCAC | 1 cut(s) | 90 |
| AoxI | GGCC | 3 cut(s) | 154, 240, 246 |
| ApaLI | GTGCAC | 1 cut(s) | 90 |
| ApeKI | GCWGC | 1 cut(s) | 141 |
| AspLEI | GCGC | 1 cut(s) | 219 |
| AsuC2I | CCSGG | 1 cut(s) | 117 |
| AsuHPI | GGTGA | 2 cut(s) | 91, 134 |
| BaeGI | GKGCMC | 1 cut(s) | 94 |
| Bbv12I | GWGCWC | 1 cut(s) | 94 |
| BbvI | GCAGC | 1 cut(s) | 153 |
| BciT130I | CCWGG | 1 cut(s) | 153 |
| BcnI | CCSGG | 1 cut(s) | 117 |
| BcoDI | GTCTC | 1 cut(s) | 119 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 153 |
| BisI | GCNGC | 2 cut(s) | 142, 145 |
| BlpI | GCTNAGC | 1 cut(s) | 192 |
| BlsI | GCNGC | 2 cut(s) | 143, 146 |
| Bme1390I | CCNGG | 2 cut(s) | 117, 153 |
| BmrFI | CCNGG | 2 cut(s) | 117, 153 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 192 |
| BpuMI | CCSGG | 1 cut(s) | 117 |
| BsaJI | CCNNGG | 2 cut(s) | 115, 243 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 73 |
| Bse1I | ACTGG | 1 cut(s) | 43 |
| BseBI | CCWGG | 1 cut(s) | 153 |
| BseDI | CCNNGG | 2 cut(s) | 115, 243 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 73 |
| BseMII | CTCAG | 1 cut(s) | 183 |
| BseNI | ACTGG | 1 cut(s) | 43 |
| BseSI | GKGCMC | 1 cut(s) | 94 |
| BseXI | GCAGC | 1 cut(s) | 153 |
| BseYI | CCCAGC | 1 cut(s) | 70 |
| Bsh1236I | CGCG | 1 cut(s) | 219 |
| BshFI | GGCC | 3 cut(s) | 156, 242, 248 |
| BsiHKAI | GWGCWC | 1 cut(s) | 94 |
| BsiSI | CCGG | 1 cut(s) | 117 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 73 |
| BsmAI | GTCTC | 1 cut(s) | 119 |
| BsnI | GGCC | 3 cut(s) | 156, 242, 248 |
| Bsp1286I | GDGCHC | 1 cut(s) | 94 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 192 |
| BspACI | CCGC | 4 cut(s) | 74, 120, 145, 149 |
| BspANI | GGCC | 3 cut(s) | 156, 242, 248 |
| BspCNI | CTCAG | 1 cut(s) | 184 |
| BspFNI | CGCG | 1 cut(s) | 219 |
| BspQI | GCTCTTC | 1 cut(s) | 24 |
| BsrI | ACTGG | 1 cut(s) | 43 |
| BssECI | CCNNGG | 2 cut(s) | 115, 243 |
| Bst2UI | CCWGG | 1 cut(s) | 153 |
| Bst4CI | ACNGT | 1 cut(s) | 89 |
| Bst6I | CTCTTC | 1 cut(s) | 24 |
| BstC8I | GCNNGC | 3 cut(s) | 149, 183, 221 |
| BstDEI | CTNAG | 1 cut(s) | 192 |
| BstFNI | CGCG | 1 cut(s) | 219 |
| BstHHI | GCGC | 1 cut(s) | 219 |
| BstMAI | GTCTC | 1 cut(s) | 119 |
| BstMWI | GCNNNNNNNGC | 1 cut(s) | 153 |
| BstNI | CCWGG | 1 cut(s) | 153 |
| BstSCI | CCNGG | 2 cut(s) | 115, 151 |
| BstSLI | GKGCMC | 1 cut(s) | 94 |
| BstUI | CGCG | 1 cut(s) | 219 |
| BstV1I | GCAGC | 1 cut(s) | 153 |
| BsuRI | GGCC | 3 cut(s) | 156, 242, 248 |
| Cac8I | GCNNGC | 3 cut(s) | 149, 183, 221 |
| CfoI | GCGC | 1 cut(s) | 219 |
| CviJI | RGCY | 8 cut(s) | 5, 144, 156, 181, 191, 196, 242, 248 |
| CviKI_1 | RGCY | 8 cut(s) | 5, 144, 156, 181, 191, 196, 242, 248 |
| DdeI | CTNAG | 1 cut(s) | 192 |
| DrdI | GACNNNNNNGTC | 2 cut(s) | 43, 47 |
| DseDI | GACNNNNNNGTC | 2 cut(s) | 43, 47 |
| Eam1104I | CTCTTC | 1 cut(s) | 24 |
| EarI | CTCTTC | 1 cut(s) | 24 |
| Eco147I | AGGCCT | 1 cut(s) | 248 |
| Eco57I | CTGAAG | 1 cut(s) | 48 |
| EcoRII | CCWGG | 1 cut(s) | 151 |
| FaiI | YATR | 2 cut(s) | 175, 177 |
| FauI | CCCGC | 1 cut(s) | 156 |
| Fnu4HI | GCNGC | 2 cut(s) | 142, 145 |
| Fsp4HI | GCNGC | 2 cut(s) | 142, 145 |
| GlaI | GCGC | 1 cut(s) | 218 |
| GluI | GCNGC | 2 cut(s) | 142, 145 |
| GsaI | CCCAGC | 1 cut(s) | 74 |
| HaeIII | GGCC | 3 cut(s) | 156, 242, 248 |
| HapII | CCGG | 1 cut(s) | 117 |
| HhaI | GCGC | 1 cut(s) | 219 |
| Hin6I | GCGC | 1 cut(s) | 217 |
| HinP1I | GCGC | 1 cut(s) | 217 |
| HincII | GTYRAC | 1 cut(s) | 85 |
| HindII | GTYRAC | 1 cut(s) | 85 |
| HpaII | CCGG | 1 cut(s) | 117 |
| HphI | GGTGA | 2 cut(s) | 91, 134 |
| Hpy166II | GTNNAC | 3 cut(s) | 79, 85, 92 |
| Hpy8I | GTNNAC | 3 cut(s) | 79, 85, 92 |
| HpyCH4III | ACNGT | 1 cut(s) | 89 |
| HpyCH4IV | ACGT | 1 cut(s) | 81 |
| HpyCH4V | TGCA | 1 cut(s) | 92 |
| HpyF10VI | GCNNNNNNNGC | 1 cut(s) | 153 |
| HpyF3I | CTNAG | 1 cut(s) | 192 |
| HpySE526I | ACGT | 1 cut(s) | 81 |
| HspAI | GCGC | 1 cut(s) | 217 |
| LguI | GCTCTTC | 1 cut(s) | 24 |
| LpnPI | CCDG | 6 cut(s) | 56, 84, 130, 138, 165, 167 |
| Lsp1109I | GCAGC | 1 cut(s) | 153 |
| MaeII | ACGT | 1 cut(s) | 81 |
| MboII | GAAGA | 1 cut(s) | 41 |
| MhlI | GDGCHC | 1 cut(s) | 94 |
| MnlI | CCTC | 3 cut(s) | 173, 178, 238 |
| MspA1I | CMGCKG | 1 cut(s) | 74 |
| MspI | CCGG | 1 cut(s) | 117 |
| MspR9I | CCNGG | 2 cut(s) | 117, 153 |
| MvaI | CCWGG | 1 cut(s) | 153 |
| MvnI | CGCG | 1 cut(s) | 219 |
| MwoI | GCNNNNNNNGC | 1 cut(s) | 153 |
| NciI | CCSGG | 1 cut(s) | 117 |
| PceI | AGGCCT | 1 cut(s) | 248 |
| PciSI | GCTCTTC | 1 cut(s) | 24 |
| PkrI | GCNGC | 2 cut(s) | 143, 146 |
| Psp6I | CCWGG | 1 cut(s) | 151 |
| PspFI | CCCAGC | 1 cut(s) | 70 |
| PspGI | CCWGG | 1 cut(s) | 151 |
| SapI | GCTCTTC | 1 cut(s) | 24 |
| SatI | GCNGC | 2 cut(s) | 142, 145 |
| ScrFI | CCNGG | 2 cut(s) | 117, 153 |
| SduI | GDGCHC | 1 cut(s) | 94 |
| SetI | ASST | 5 cut(s) | 64, 84, 165, 183, 198 |
| SseBI | AGGCCT | 1 cut(s) | 248 |
| SsiI | CCGC | 4 cut(s) | 74, 120, 145, 149 |
| StuI | AGGCCT | 1 cut(s) | 248 |
| StyD4I | CCNGG | 2 cut(s) | 115, 151 |
| TaaI | ACNGT | 1 cut(s) | 89 |
| TaiI | ACGT | 1 cut(s) | 84 |
| TauI | GCSGC | 1 cut(s) | 147 |
| TseI | GCWGC | 1 cut(s) | 141 |
| VneI | GTGCAC | 1 cut(s) | 90 |