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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 2 cut(s) | 4, 16 |
| AccB1I | GGYRCC | 3 cut(s) | 4, 16, 991 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 151 |
| AccBSI | CCGCTC | 3 cut(s) | 669, 707, 1071 |
| AccII | CGCG | 4 cut(s) | 122, 250, 857, 1090 |
| AclWI | GGATC | 4 cut(s) | 101, 450, 727, 802 |
| AcoI | YGGCCR | 3 cut(s) | 54, 510, 894 |
| AcuI | CTGAAG | 1 cut(s) | 1065 |
| AcvI | CACGTG | 1 cut(s) | 116 |
| AcyI | GRCGYC | 1 cut(s) | 264 |
| AdeI | CACNNNGTG | 2 cut(s) | 578, 1063 |
| AfaI | GTAC | 3 cut(s) | 6, 18, 736 |
| AfiI | CCNNNNNNNGG | 8 cut(s) | 31, 151, 219, 326, 532, 617, 711, 716 |
| AgsI | TTSAA | 2 cut(s) | 843, 1016 |
| AhdI | GACNNNNNGTC | 1 cut(s) | 268 |
| AjnI | CCWGG | 8 cut(s) | 78, 89, 382, 400, 596, 610, 710, 860 |
| AleI | CACNNNNGTG | 1 cut(s) | 518 |
| AluBI | AGCT | 4 cut(s) | 142, 430, 538, 767 |
| AluI | AGCT | 4 cut(s) | 142, 430, 538, 767 |
| Alw26I | GTCTC | 4 cut(s) | 258, 276, 368, 667 |
| AlwI | GGATC | 4 cut(s) | 101, 450, 727, 802 |
| Ama87I | CYCGRG | 2 cut(s) | 670, 678 |
| AoxI | GGCC | 6 cut(s) | 54, 403, 489, 495, 510, 894 |
| ApeKI | GCWGC | 2 cut(s) | 283, 881 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 1050 |
| Asp718I | GGTACC | 2 cut(s) | 4, 16 |
| AspS9I | GGNCC | 3 cut(s) | 82, 496, 869 |
| AsuC2I | CCSGG | 6 cut(s) | 356, 366, 569, 812, 968, 989 |
| AsuHPI | GGTGA | 5 cut(s) | 88, 202, 340, 383, 868 |
| AsuII | TTCGAA | 1 cut(s) | 73 |
| AvaI | CYCGRG | 2 cut(s) | 670, 678 |
| AvaII | GGWCC | 2 cut(s) | 82, 869 |
| BaeGI | GKGCMC | 2 cut(s) | 994, 1032 |
| BalI | TGGCCA | 1 cut(s) | 512 |
| BanI | GGYRCC | 3 cut(s) | 4, 16, 991 |
| BauI | CACGAG | 1 cut(s) | 275 |
| BbrPI | CACGTG | 1 cut(s) | 116 |
| BbsI | GAAGAC | 2 cut(s) | 243, 923 |
| BbvI | GCAGC | 2 cut(s) | 295, 868 |
| BccI | CCATC | 1 cut(s) | 123 |
| BceAI | ACGGC | 2 cut(s) | 69, 881 |
| BciT130I | CCWGG | 8 cut(s) | 80, 91, 384, 402, 598, 612, 712, 862 |
| BcnI | CCSGG | 6 cut(s) | 356, 366, 569, 812, 968, 989 |
| BcoDI | GTCTC | 4 cut(s) | 258, 276, 368, 667 |
| BfaI | CTAG | 2 cut(s) | 738, 743 |
| BglI | GCCNNNNNGGC | 4 cut(s) | 62, 402, 569, 612 |
| BisI | GCNGC | 7 cut(s) | 120, 284, 882, 943, 1072, 1075, 1088 |
| BlsI | GCNGC | 7 cut(s) | 121, 285, 883, 944, 1073, 1076, 1089 |
| BmcAI | AGTACT | 1 cut(s) | 736 |
| Bme18I | GGWCC | 2 cut(s) | 82, 869 |
| BmeRI | GACNNNNNGTC | 1 cut(s) | 268 |
| BmeT110I | CYCGRG | 2 cut(s) | 670, 678 |
| BmgT120I | GGNCC | 3 cut(s) | 82, 496, 869 |
| BmiI | GGNNCC | 4 cut(s) | 6, 18, 294, 993 |
| BmsI | GCATC | 4 cut(s) | 142, 748, 774, 1086 |
| BpiI | GAAGAC | 2 cut(s) | 243, 923 |
| BpmI | CTGGAG | 2 cut(s) | 128, 572 |
| Bpu14I | TTCGAA | 1 cut(s) | 73 |
| BpuMI | CCSGG | 6 cut(s) | 356, 366, 569, 812, 968, 989 |
| BsaAI | YACGTR | 1 cut(s) | 116 |
| BsaHI | GRCGYC | 1 cut(s) | 264 |
| BsaI | GGTCTC | 2 cut(s) | 258, 667 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 238, 268 |
| Bsc4I | CCNNNNNNNGG | 8 cut(s) | 31, 151, 219, 326, 532, 617, 711, 716 |
| Bse118I | RCCGGY | 2 cut(s) | 752, 904 |
| Bse1I | ACTGG | 2 cut(s) | 441, 780 |
| BseBI | CCWGG | 8 cut(s) | 80, 91, 384, 402, 598, 612, 712, 862 |
| BseGI | GGATG | 1 cut(s) | 973 |
| BseLI | CCNNNNNNNGG | 8 cut(s) | 31, 151, 219, 326, 532, 617, 711, 716 |
| BseNI | ACTGG | 2 cut(s) | 441, 780 |
| BseRI | GAGGAG | 1 cut(s) | 548 |
| BseSI | GKGCMC | 2 cut(s) | 994, 1032 |
| BseXI | GCAGC | 2 cut(s) | 295, 868 |
| BseYI | CCCAGC | 2 cut(s) | 319, 829 |
| BsgI | GTGCAG | 1 cut(s) | 641 |
| Bsh1236I | CGCG | 4 cut(s) | 122, 250, 857, 1090 |
| BshFI | GGCC | 6 cut(s) | 56, 405, 491, 497, 512, 896 |
| BshNI | GGYRCC | 3 cut(s) | 4, 16, 991 |
| BsiHKCI | CYCGRG | 2 cut(s) | 670, 678 |
| BsiSI | CCGG | 8 cut(s) | 356, 366, 569, 753, 811, 905, 968, 988 |
| BslFI | GGGAC | 2 cut(s) | 157, 508 |
| BslI | CCNNNNNNNGG | 8 cut(s) | 31, 151, 219, 326, 532, 617, 711, 716 |
| BsmAI | GTCTC | 4 cut(s) | 258, 276, 368, 667 |
| BsmFI | GGGAC | 2 cut(s) | 157, 508 |
| BsnI | GGCC | 6 cut(s) | 56, 405, 491, 497, 512, 896 |
| Bso31I | GGTCTC | 2 cut(s) | 258, 667 |
| BsoBI | CYCGRG | 2 cut(s) | 670, 678 |
| Bsp119I | TTCGAA | 1 cut(s) | 73 |
| Bsp1286I | GDGCHC | 2 cut(s) | 994, 1032 |
| Bsp143I | GATC | 6 cut(s) | 93, 195, 442, 719, 807, 1091 |
| BspANI | GGCC | 6 cut(s) | 56, 405, 491, 497, 512, 896 |
| BspFNI | CGCG | 4 cut(s) | 122, 250, 857, 1090 |
| BspLI | GGNNCC | 4 cut(s) | 6, 18, 294, 993 |
| BspPI | GGATC | 4 cut(s) | 101, 450, 727, 802 |
| BspT104I | TTCGAA | 1 cut(s) | 73 |
| BspT107I | GGYRCC | 3 cut(s) | 4, 16, 991 |
| BspTNI | GGTCTC | 2 cut(s) | 258, 667 |
| BsrBI | CCGCTC | 3 cut(s) | 669, 707, 1071 |
| BsrFI | RCCGGY | 2 cut(s) | 752, 904 |
| BsrI | ACTGG | 2 cut(s) | 441, 780 |
| BssAI | RCCGGY | 2 cut(s) | 752, 904 |
| BssMI | GATC | 6 cut(s) | 93, 195, 442, 719, 807, 1091 |
| BssNI | GRCGYC | 1 cut(s) | 264 |
| BssSI | CACGAG | 1 cut(s) | 275 |
| BssT1I | CCWWGG | 1 cut(s) | 30 |
| Bst2BI | CACGAG | 1 cut(s) | 275 |
| Bst2UI | CCWGG | 8 cut(s) | 80, 91, 384, 402, 598, 612, 712, 862 |
| Bst4CI | ACNGT | 3 cut(s) | 70, 792, 982 |
| BstACI | GRCGYC | 1 cut(s) | 264 |
| BstBAI | YACGTR | 1 cut(s) | 116 |
| BstBI | TTCGAA | 1 cut(s) | 73 |
| BstC8I | GCNNGC | 7 cut(s) | 288, 398, 432, 754, 894, 955, 1069 |
| BstDSI | CCRYGG | 1 cut(s) | 43 |
| BstEII | GGTNACC | 1 cut(s) | 37 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 217, 615 |
| BstF5I | GGATG | 1 cut(s) | 973 |
| BstFNI | CGCG | 4 cut(s) | 122, 250, 857, 1090 |
| BstKTI | GATC | 6 cut(s) | 96, 198, 445, 722, 810, 1094 |
| BstMAI | GTCTC | 4 cut(s) | 258, 276, 368, 667 |
| BstMBI | GATC | 6 cut(s) | 93, 195, 442, 719, 807, 1091 |
| BstMWI | GCNNNNNNNGC | 9 cut(s) | 62, 139, 296, 336, 402, 569, 612, 641, 758 |
| BstNI | CCWGG | 8 cut(s) | 80, 91, 384, 402, 598, 612, 712, 862 |
| BstPI | GGTNACC | 1 cut(s) | 37 |
| BstSLI | GKGCMC | 2 cut(s) | 994, 1032 |
| BstUI | CGCG | 4 cut(s) | 122, 250, 857, 1090 |
| BstV1I | GCAGC | 2 cut(s) | 295, 868 |
| BstV2I | GAAGAC | 2 cut(s) | 243, 923 |
| BstX2I | RGATCY | 1 cut(s) | 442 |
| BstYI | RGATCY | 1 cut(s) | 442 |
| BsuRI | GGCC | 6 cut(s) | 56, 405, 491, 497, 512, 896 |
| BtgI | CCRYGG | 1 cut(s) | 43 |
| BtgZI | GCGATG | 1 cut(s) | 771 |
| BtsCI | GGATG | 1 cut(s) | 973 |
| BtsI | GCAGTG | 1 cut(s) | 653 |
| BtsIMutI | CAGTG | 3 cut(s) | 434, 653, 1041 |
| Cac8I | GCNNGC | 7 cut(s) | 288, 398, 432, 754, 894, 955, 1069 |
| Cfr10I | RCCGGY | 2 cut(s) | 752, 904 |
| Cfr13I | GGNCC | 3 cut(s) | 82, 496, 869 |
| CseI | GACGC | 4 cut(s) | 111, 113, 272, 846 |
| Csp6I | GTAC | 3 cut(s) | 5, 17, 735 |
| CviQI | GTAC | 3 cut(s) | 5, 17, 735 |
| DpnI | GATC | 6 cut(s) | 95, 197, 444, 721, 809, 1093 |
| DpnII | GATC | 6 cut(s) | 93, 195, 442, 719, 807, 1091 |
| DraIII | CACNNNGTG | 2 cut(s) | 578, 1063 |
| DriI | GACNNNNNGTC | 1 cut(s) | 268 |
| EaeI | YGGCCR | 3 cut(s) | 54, 510, 894 |
| Eam1105I | GACNNNNNGTC | 1 cut(s) | 268 |
| EciI | GGCGGA | 4 cut(s) | 99, 455, 830, 974 |
| Eco130I | CCWWGG | 1 cut(s) | 30 |
| Eco31I | GGTCTC | 2 cut(s) | 258, 667 |
| Eco47I | GGWCC | 2 cut(s) | 82, 869 |
| Eco57I | CTGAAG | 1 cut(s) | 1065 |
| Eco72I | CACGTG | 1 cut(s) | 116 |
| Eco88I | CYCGRG | 2 cut(s) | 670, 678 |
| Eco91I | GGTNACC | 1 cut(s) | 37 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 217, 615 |
| EcoO65I | GGTNACC | 1 cut(s) | 37 |
| EcoRII | CCWGG | 8 cut(s) | 78, 89, 382, 400, 596, 610, 710, 860 |
| EcoT14I | CCWWGG | 1 cut(s) | 30 |
| ErhI | CCWWGG | 1 cut(s) | 30 |
| FaiI | YATR | 3 cut(s) | 216, 426, 912 |
| FaqI | GGGAC | 2 cut(s) | 157, 508 |
| FauI | CCCGC | 4 cut(s) | 304, 533, 712, 757 |
| Fnu4HI | GCNGC | 7 cut(s) | 120, 284, 882, 943, 1072, 1075, 1088 |
| FokI | GGATG | 1 cut(s) | 960 |
| Fsp4HI | GCNGC | 7 cut(s) | 120, 284, 882, 943, 1072, 1075, 1088 |
| FspBI | CTAG | 2 cut(s) | 738, 743 |
| GluI | GCNGC | 7 cut(s) | 120, 284, 882, 943, 1072, 1075, 1088 |
| GsaI | CCCAGC | 2 cut(s) | 323, 833 |
| GsuI | CTGGAG | 2 cut(s) | 128, 572 |
| HaeIII | GGCC | 6 cut(s) | 56, 405, 491, 497, 512, 896 |
| HapII | CCGG | 8 cut(s) | 356, 366, 569, 753, 811, 905, 968, 988 |
| HgaI | GACGC | 4 cut(s) | 111, 113, 272, 846 |
| Hin1I | GRCGYC | 1 cut(s) | 264 |
| HincII | GTYRAC | 1 cut(s) | 361 |
| HindII | GTYRAC | 1 cut(s) | 361 |
| HinfI | GANTC | 5 cut(s) | 156, 647, 818, 825, 919 |
| HpaII | CCGG | 8 cut(s) | 356, 366, 569, 753, 811, 905, 968, 988 |
| HphI | GGTGA | 5 cut(s) | 88, 202, 340, 383, 868 |
| Hpy166II | GTNNAC | 2 cut(s) | 361, 470 |
| Hpy188I | TCNGA | 4 cut(s) | 138, 182, 262, 902 |
| Hpy188III | TCNNGA | 7 cut(s) | 126, 145, 588, 672, 822, 867, 1095 |
| Hpy8I | GTNNAC | 2 cut(s) | 361, 470 |
| HpyAV | CCTTC | 3 cut(s) | 214, 481, 1040 |
| HpyCH4III | ACNGT | 3 cut(s) | 70, 792, 982 |
| HpyCH4IV | ACGT | 2 cut(s) | 115, 149 |
| HpyCH4V | TGCA | 5 cut(s) | 283, 658, 761, 787, 884 |
| HpyF10VI | GCNNNNNNNGC | 9 cut(s) | 62, 139, 296, 336, 402, 569, 612, 641, 758 |
| HpySE526I | ACGT | 2 cut(s) | 115, 149 |
| Hsp92I | GRCGYC | 1 cut(s) | 264 |
| KpnI | GGTACC | 2 cut(s) | 8, 20 |
| KroI | GCCGGC | 1 cut(s) | 752 |
| KroNI | GCCGGC | 1 cut(s) | 754 |
| Kzo9I | GATC | 6 cut(s) | 93, 195, 442, 719, 807, 1091 |
| LmnI | GCTCC | 5 cut(s) | 112, 147, 535, 620, 712 |
| Lsp1109I | GCAGC | 2 cut(s) | 295, 868 |
| LweI | GCATC | 4 cut(s) | 142, 748, 774, 1086 |
| MaeI | CTAG | 2 cut(s) | 738, 743 |
| MaeII | ACGT | 2 cut(s) | 115, 149 |
| MaeIII | GTNAC | 4 cut(s) | 37, 585, 930, 1055 |
| MalI | GATC | 6 cut(s) | 95, 197, 444, 721, 809, 1093 |
| MbiI | CCGCTC | 3 cut(s) | 669, 707, 1071 |
| MboI | GATC | 6 cut(s) | 93, 195, 442, 719, 807, 1091 |
| MboII | GAAGA | 5 cut(s) | 202, 205, 248, 437, 928 |
| MflI | RGATCY | 1 cut(s) | 442 |
| MhlI | GDGCHC | 2 cut(s) | 994, 1032 |
| MlsI | TGGCCA | 1 cut(s) | 512 |
| MluCI | AATT | 2 cut(s) | 885, 1008 |
| MluNI | TGGCCA | 1 cut(s) | 512 |
| MlyI | GAGTC | 2 cut(s) | 656, 913 |
| MmeI | TCCRAC | 1 cut(s) | 774 |
| Mox20I | TGGCCA | 1 cut(s) | 512 |
| MreI | CGCCGGCG | 1 cut(s) | 752 |
| MroNI | GCCGGC | 1 cut(s) | 752 |
| MroXI | GAANNNNTTC | 1 cut(s) | 1050 |
| MscI | TGGCCA | 1 cut(s) | 512 |
| MseI | TTAA | 1 cut(s) | 333 |
| MslI | CAYNNNNRTG | 2 cut(s) | 518, 1082 |
| Msp20I | TGGCCA | 1 cut(s) | 512 |
| MspA1I | CMGCKG | 5 cut(s) | 101, 323, 730, 881, 942 |
| MspI | CCGG | 8 cut(s) | 356, 366, 569, 753, 811, 905, 968, 988 |
| MvaI | CCWGG | 8 cut(s) | 80, 91, 384, 402, 598, 612, 712, 862 |
| MvnI | CGCG | 4 cut(s) | 122, 250, 857, 1090 |
| MwoI | GCNNNNNNNGC | 9 cut(s) | 62, 139, 296, 336, 402, 569, 612, 641, 758 |
| NaeI | GCCGGC | 1 cut(s) | 754 |
| NciI | CCSGG | 6 cut(s) | 356, 366, 569, 812, 968, 989 |
| NdeII | GATC | 6 cut(s) | 93, 195, 442, 719, 807, 1091 |
| NgoMIV | GCCGGC | 1 cut(s) | 752 |
| NlaIV | GGNNCC | 4 cut(s) | 6, 18, 294, 993 |
| NmeAIII | GCCGAG | 1 cut(s) | 517 |
| NmuCI | GTSAC | 3 cut(s) | 585, 930, 1055 |
| NspV | TTCGAA | 1 cut(s) | 73 |
| OliI | CACNNNNGTG | 1 cut(s) | 518 |
| PaeR7I | CTCGAG | 1 cut(s) | 670 |
| PdiI | GCCGGC | 1 cut(s) | 754 |
| PdmI | GAANNNNTTC | 1 cut(s) | 1050 |
| PfeI | GAWTC | 3 cut(s) | 156, 818, 825 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 151 |
| PkrI | GCNGC | 7 cut(s) | 121, 285, 883, 944, 1073, 1076, 1089 |
| PleI | GAGTC | 2 cut(s) | 655, 913 |
| PmaCI | CACGTG | 1 cut(s) | 116 |
| PmlI | CACGTG | 1 cut(s) | 116 |
| PpsI | GAGTC | 2 cut(s) | 655, 913 |
| Ppu21I | YACGTR | 1 cut(s) | 116 |
| Psp6I | CCWGG | 8 cut(s) | 78, 89, 382, 400, 596, 610, 710, 860 |
| PspCI | CACGTG | 1 cut(s) | 116 |
| PspEI | GGTNACC | 1 cut(s) | 37 |
| PspFI | CCCAGC | 2 cut(s) | 319, 829 |
| PspGI | CCWGG | 8 cut(s) | 78, 89, 382, 400, 596, 610, 710, 860 |
| PspN4I | GGNNCC | 4 cut(s) | 6, 18, 294, 993 |
| PspPI | GGNCC | 3 cut(s) | 82, 496, 869 |
| PsuI | RGATCY | 1 cut(s) | 442 |
| RsaI | GTAC | 3 cut(s) | 6, 18, 736 |
| RsaNI | GTAC | 3 cut(s) | 5, 17, 735 |
| RseI | CAYNNNNRTG | 2 cut(s) | 518, 1082 |
| SaqAI | TTAA | 1 cut(s) | 333 |
| SatI | GCNGC | 7 cut(s) | 120, 284, 882, 943, 1072, 1075, 1088 |
| Sau3AI | GATC | 6 cut(s) | 93, 195, 442, 719, 807, 1091 |
| Sau96I | GGNCC | 3 cut(s) | 82, 496, 869 |
| ScaI | AGTACT | 1 cut(s) | 736 |
| SchI | GAGTC | 2 cut(s) | 656, 913 |
| SduI | GDGCHC | 2 cut(s) | 994, 1032 |
| SfaNI | GCATC | 4 cut(s) | 142, 748, 774, 1086 |
| Sfr274I | CTCGAG | 1 cut(s) | 670 |
| SfuI | TTCGAA | 1 cut(s) | 73 |
| SgrAI | CRCCGGYG | 1 cut(s) | 752 |
| SinI | GGWCC | 2 cut(s) | 82, 869 |
| SlaI | CTCGAG | 1 cut(s) | 670 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 518, 1082 |
| SmlI | CTYRAG | 1 cut(s) | 670 |
| SmoI | CTYRAG | 1 cut(s) | 670 |
| Sse9I | AATT | 2 cut(s) | 885, 1008 |
| SspMI | CTAG | 2 cut(s) | 738, 743 |
| StyI | CCWWGG | 1 cut(s) | 30 |
| TaaI | ACNGT | 3 cut(s) | 70, 792, 982 |
| TaiI | ACGT | 2 cut(s) | 118, 152 |
| TaqI | TCGA | 3 cut(s) | 73, 159, 671 |
| TasI | AATT | 2 cut(s) | 885, 1008 |
| TatI | WGTACW | 1 cut(s) | 734 |
| TauI | GCSGC | 5 cut(s) | 122, 945, 1074, 1077, 1090 |
| TfiI | GAWTC | 3 cut(s) | 156, 818, 825 |
| Tru1I | TTAA | 1 cut(s) | 333 |
| Tru9I | TTAA | 1 cut(s) | 333 |
| TscAI | CASTG | 3 cut(s) | 441, 660, 1048 |
| TseFI | GTSAC | 3 cut(s) | 585, 930, 1055 |
| TseI | GCWGC | 2 cut(s) | 283, 881 |
| Tsp45I | GTSAC | 3 cut(s) | 585, 930, 1055 |
| TspDTI | ATGAA | 1 cut(s) | 962 |
| TspGWI | ACGGA | 1 cut(s) | 1047 |
| TspRI | CASTG | 3 cut(s) | 441, 660, 1048 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 151 |
| VpaK11BI | GGWCC | 2 cut(s) | 82, 869 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 217, 615 |
| XhoI | CTCGAG | 1 cut(s) | 670 |
| XmnI | GAANNNNTTC | 1 cut(s) | 1050 |
| XspI | CTAG | 2 cut(s) | 738, 743 |
| ZrmI | AGTACT | 1 cut(s) | 736 |