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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 1 cut(s) | 76 |
| Acc36I | ACCTGC | 2 cut(s) | 76, 222 |
| Acc65I | GGTACC | 2 cut(s) | 207, 234 |
| AccB1I | GGYRCC | 2 cut(s) | 207, 234 |
| AccI | GTMKAC | 1 cut(s) | 186 |
| AccII | CGCG | 1 cut(s) | 97 |
| AciI | CCGC | 4 cut(s) | 20, 97, 238, 285 |
| AcsI | RAATTY | 1 cut(s) | 107 |
| AfaI | GTAC | 3 cut(s) | 209, 215, 236 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 31, 85, 196 |
| AluBI | AGCT | 2 cut(s) | 10, 46 |
| AluI | AGCT | 2 cut(s) | 10, 46 |
| Alw21I | GWGCWC | 2 cut(s) | 48, 175 |
| AoxI | GGCC | 2 cut(s) | 75, 267 |
| ApaI | GGGCCC | 1 cut(s) | 271 |
| ApoI | RAATTY | 1 cut(s) | 107 |
| Asp718I | GGTACC | 2 cut(s) | 207, 234 |
| AspLEI | GCGC | 2 cut(s) | 60, 130 |
| AspS9I | GGNCC | 4 cut(s) | 22, 76, 267, 268 |
| AsuHPI | GGTGA | 1 cut(s) | 83 |
| AvaII | GGWCC | 1 cut(s) | 22 |
| BaeGI | GKGCMC | 1 cut(s) | 271 |
| BanI | GGYRCC | 2 cut(s) | 207, 234 |
| BanII | GRGCYC | 2 cut(s) | 48, 271 |
| Bbv12I | GWGCWC | 2 cut(s) | 48, 175 |
| BfoI | RGCGCY | 1 cut(s) | 131 |
| BfuAI | ACCTGC | 2 cut(s) | 76, 222 |
| Bme18I | GGWCC | 1 cut(s) | 22 |
| BmgT120I | GGNCC | 4 cut(s) | 22, 76, 267, 268 |
| BmiI | GGNNCC | 3 cut(s) | 209, 236, 269 |
| BmsI | GCATC | 1 cut(s) | 218 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 31, 85, 196 |
| Bse1I | ACTGG | 1 cut(s) | 201 |
| BseGI | GGATG | 1 cut(s) | 105 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 31, 85, 196 |
| BseNI | ACTGG | 1 cut(s) | 201 |
| BseSI | GKGCMC | 1 cut(s) | 271 |
| Bsh1236I | CGCG | 1 cut(s) | 97 |
| BshFI | GGCC | 2 cut(s) | 77, 269 |
| BshNI | GGYRCC | 2 cut(s) | 207, 234 |
| BsiHKAI | GWGCWC | 2 cut(s) | 48, 175 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 31, 85, 196 |
| BsnI | GGCC | 2 cut(s) | 77, 269 |
| Bsp120I | GGGCCC | 1 cut(s) | 267 |
| Bsp1286I | GDGCHC | 3 cut(s) | 48, 175, 271 |
| BspACI | CCGC | 4 cut(s) | 20, 97, 238, 285 |
| BspANI | GGCC | 2 cut(s) | 77, 269 |
| BspFNI | CGCG | 1 cut(s) | 97 |
| BspLI | GGNNCC | 3 cut(s) | 209, 236, 269 |
| BspMI | ACCTGC | 2 cut(s) | 76, 222 |
| BspT107I | GGYRCC | 2 cut(s) | 207, 234 |
| BsrI | ACTGG | 1 cut(s) | 201 |
| BstC8I | GCNNGC | 2 cut(s) | 12, 160 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 29 |
| BstF5I | GGATG | 1 cut(s) | 105 |
| BstFNI | CGCG | 1 cut(s) | 97 |
| BstH2I | RGCGCY | 1 cut(s) | 131 |
| BstHHI | GCGC | 2 cut(s) | 60, 130 |
| BstMWI | GCNNNNNNNGC | 2 cut(s) | 148, 237 |
| BstSLI | GKGCMC | 1 cut(s) | 271 |
| BstUI | CGCG | 1 cut(s) | 97 |
| BsuRI | GGCC | 2 cut(s) | 77, 269 |
| BtsCI | GGATG | 1 cut(s) | 105 |
| BveI | ACCTGC | 2 cut(s) | 76, 222 |
| Cac8I | GCNNGC | 2 cut(s) | 12, 160 |
| CfoI | GCGC | 2 cut(s) | 60, 130 |
| Cfr13I | GGNCC | 4 cut(s) | 22, 76, 267, 268 |
| Csp6I | GTAC | 3 cut(s) | 208, 214, 235 |
| CviJI | RGCY | 4 cut(s) | 10, 46, 77, 269 |
| CviKI_1 | RGCY | 4 cut(s) | 10, 46, 77, 269 |
| CviQI | GTAC | 3 cut(s) | 208, 214, 235 |
| Ecl136II | GAGCTC | 1 cut(s) | 46 |
| Eco24I | GRGCYC | 2 cut(s) | 48, 271 |
| Eco47I | GGWCC | 1 cut(s) | 22 |
| Eco53kI | GAGCTC | 1 cut(s) | 46 |
| EcoICRI | GAGCTC | 1 cut(s) | 46 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 29 |
| EcoT38I | GRGCYC | 2 cut(s) | 48, 271 |
| FaiI | YATR | 2 cut(s) | 6, 223 |
| FblI | GTMKAC | 1 cut(s) | 186 |
| FokI | GGATG | 1 cut(s) | 112 |
| FriOI | GRGCYC | 2 cut(s) | 48, 271 |
| GlaI | GCGC | 2 cut(s) | 59, 129 |
| HaeII | RGCGCY | 1 cut(s) | 131 |
| HaeIII | GGCC | 2 cut(s) | 77, 269 |
| HhaI | GCGC | 2 cut(s) | 60, 130 |
| Hin6I | GCGC | 2 cut(s) | 58, 128 |
| HinP1I | GCGC | 2 cut(s) | 58, 128 |
| HincII | GTYRAC | 1 cut(s) | 187 |
| HindII | GTYRAC | 1 cut(s) | 187 |
| HphI | GGTGA | 1 cut(s) | 83 |
| Hpy166II | GTNNAC | 1 cut(s) | 187 |
| Hpy8I | GTNNAC | 1 cut(s) | 187 |
| Hpy99I | CGWCG | 1 cut(s) | 116 |
| HpyCH4IV | ACGT | 1 cut(s) | 180 |
| HpyCH4V | TGCA | 1 cut(s) | 231 |
| HpyF10VI | GCNNNNNNNGC | 2 cut(s) | 148, 237 |
| HpySE526I | ACGT | 1 cut(s) | 180 |
| HspAI | GCGC | 2 cut(s) | 58, 128 |
| KpnI | GGTACC | 2 cut(s) | 211, 238 |
| LmnI | GCTCC | 2 cut(s) | 43, 170 |
| LpnPI | CCDG | 8 cut(s) | 25, 71, 116, 161, 166, 182, 217, 263 |
| LweI | GCATC | 1 cut(s) | 218 |
| MaeII | ACGT | 1 cut(s) | 180 |
| MhlI | GDGCHC | 3 cut(s) | 48, 175, 271 |
| MluCI | AATT | 2 cut(s) | 107, 202 |
| MnlI | CCTC | 4 cut(s) | 35, 67, 117, 284 |
| MseI | TTAA | 1 cut(s) | 119 |
| MslI | CAYNNNNRTG | 1 cut(s) | 226 |
| MvnI | CGCG | 1 cut(s) | 97 |
| MwoI | GCNNNNNNNGC | 2 cut(s) | 148, 237 |
| NlaIV | GGNNCC | 3 cut(s) | 209, 236, 269 |
| PaqCI | CACCTGC | 1 cut(s) | 76 |
| Psp124BI | GAGCTC | 1 cut(s) | 48 |
| PspN4I | GGNNCC | 3 cut(s) | 209, 236, 269 |
| PspOMI | GGGCCC | 1 cut(s) | 267 |
| PspPI | GGNCC | 4 cut(s) | 22, 76, 267, 268 |
| RsaI | GTAC | 3 cut(s) | 209, 215, 236 |
| RsaNI | GTAC | 3 cut(s) | 208, 214, 235 |
| RseI | CAYNNNNRTG | 1 cut(s) | 226 |
| SacI | GAGCTC | 1 cut(s) | 48 |
| SalI | GTCGAC | 1 cut(s) | 185 |
| SaqAI | TTAA | 1 cut(s) | 119 |
| Sau96I | GGNCC | 4 cut(s) | 22, 76, 267, 268 |
| SduI | GDGCHC | 3 cut(s) | 48, 175, 271 |
| SetI | ASST | 7 cut(s) | 12, 27, 48, 90, 183, 236, 276 |
| SfaNI | GCATC | 1 cut(s) | 218 |
| SinI | GGWCC | 1 cut(s) | 22 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 226 |
| Sse9I | AATT | 2 cut(s) | 107, 202 |
| SsiI | CCGC | 4 cut(s) | 20, 97, 238, 285 |
| SstI | GAGCTC | 1 cut(s) | 48 |
| TaiI | ACGT | 1 cut(s) | 183 |
| TaqI | TCGA | 2 cut(s) | 111, 186 |
| TasI | AATT | 2 cut(s) | 107, 202 |
| Tru1I | TTAA | 1 cut(s) | 119 |
| Tru9I | TTAA | 1 cut(s) | 119 |
| TspGWI | ACGGA | 1 cut(s) | 183 |
| VpaK11BI | GGWCC | 1 cut(s) | 22 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 29 |
| XapI | RAATTY | 1 cut(s) | 107 |
| XmiI | GTMKAC | 1 cut(s) | 186 |