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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AarI | CACCTGC | 2 cut(s) | 214, 514 |
| Acc36I | ACCTGC | 3 cut(s) | 214, 361, 514 |
| Acc65I | GGTACC | 1 cut(s) | 346 |
| AccB1I | GGYRCC | 1 cut(s) | 346 |
| AccBSI | CCGCTC | 1 cut(s) | 471 |
| AccII | CGCG | 1 cut(s) | 57 |
| AclWI | GGATC | 4 cut(s) | 168, 529, 660, 727 |
| AcoI | YGGCCR | 1 cut(s) | 228 |
| AcuI | CTGAAG | 1 cut(s) | 23 |
| AfaI | GTAC | 3 cut(s) | 334, 348, 691 |
| AfiI | CCNNNNNNNGG | 4 cut(s) | 223, 335, 475, 518 |
| AgsI | TTSAA | 3 cut(s) | 188, 623, 759 |
| AjnI | CCWGG | 3 cut(s) | 118, 314, 640 |
| AleI | CACNNNNGTG | 1 cut(s) | 236 |
| AluBI | AGCT | 4 cut(s) | 148, 184, 394, 427 |
| AluI | AGCT | 4 cut(s) | 148, 184, 394, 427 |
| Alw21I | GWGCWC | 1 cut(s) | 314 |
| Alw26I | GTCTC | 1 cut(s) | 86 |
| AlwI | GGATC | 4 cut(s) | 168, 529, 660, 727 |
| AoxI | GGCC | 7 cut(s) | 121, 213, 228, 326, 407, 547, 682 |
| ApeKI | GCWGC | 4 cut(s) | 108, 589, 610, 661 |
| Asp718I | GGTACC | 1 cut(s) | 346 |
| AspA2I | CCTAGG | 1 cut(s) | 512 |
| AspLEI | GCGC | 3 cut(s) | 59, 341, 615 |
| AspS9I | GGNCC | 4 cut(s) | 214, 373, 548, 649 |
| AsuC2I | CCSGG | 4 cut(s) | 74, 84, 287, 541 |
| AsuHPI | GGTGA | 4 cut(s) | 101, 124, 648, 759 |
| AvaII | GGWCC | 2 cut(s) | 373, 649 |
| AvrII | CCTAGG | 1 cut(s) | 512 |
| BaeGI | GKGCMC | 1 cut(s) | 423 |
| BalI | TGGCCA | 1 cut(s) | 230 |
| BanI | GGYRCC | 1 cut(s) | 346 |
| BauI | CACGAG | 1 cut(s) | 472 |
| Bbv12I | GWGCWC | 1 cut(s) | 314 |
| BbvI | GCAGC | 4 cut(s) | 120, 601, 622, 648 |
| BcgI | CGANNNNNNTGC | 2 cut(s) | 435, 469 |
| BciT130I | CCWGG | 3 cut(s) | 120, 316, 642 |
| BcnI | CCSGG | 4 cut(s) | 74, 84, 287, 541 |
| BcoDI | GTCTC | 1 cut(s) | 86 |
| BfaI | CTAG | 3 cut(s) | 330, 480, 513 |
| BfmI | CTRYAG | 1 cut(s) | 670 |
| BfoI | RGCGCY | 1 cut(s) | 616 |
| BfuAI | ACCTGC | 3 cut(s) | 214, 361, 514 |
| BglI | GCCNNNNNGGC | 3 cut(s) | 120, 287, 555 |
| BisI | GCNGC | 5 cut(s) | 109, 112, 590, 611, 662 |
| BlnI | CCTAGG | 1 cut(s) | 512 |
| BlsI | GCNGC | 5 cut(s) | 110, 113, 591, 612, 663 |
| Bme1390I | CCNGG | 7 cut(s) | 74, 84, 120, 287, 316, 541, 642 |
| Bme18I | GGWCC | 2 cut(s) | 373, 649 |
| BmgT120I | GGNCC | 4 cut(s) | 214, 373, 548, 649 |
| BmiI | GGNNCC | 3 cut(s) | 348, 375, 550 |
| BmrFI | CCNGG | 7 cut(s) | 74, 84, 120, 287, 316, 541, 642 |
| BmrI | ACTGGG | 1 cut(s) | 32 |
| BmsI | GCATC | 3 cut(s) | 57, 576, 597 |
| BmuI | ACTGGG | 1 cut(s) | 32 |
| BpmI | CTGGAG | 1 cut(s) | 290 |
| BpuMI | CCSGG | 4 cut(s) | 74, 84, 287, 541 |
| BsaJI | CCNNGG | 4 cut(s) | 72, 82, 210, 512 |
| Bsc4I | CCNNNNNNNGG | 4 cut(s) | 223, 335, 475, 518 |
| Bse118I | RCCGGY | 3 cut(s) | 324, 335, 684 |
| Bse1I | ACTGG | 5 cut(s) | 18, 38, 159, 356, 582 |
| BseBI | CCWGG | 3 cut(s) | 120, 316, 642 |
| BseDI | CCNNGG | 4 cut(s) | 72, 82, 210, 512 |
| BseGI | GGATG | 2 cut(s) | 591, 612 |
| BseLI | CCNNNNNNNGG | 4 cut(s) | 223, 335, 475, 518 |
| BseNI | ACTGG | 5 cut(s) | 18, 38, 159, 356, 582 |
| BseSI | GKGCMC | 1 cut(s) | 423 |
| BseXI | GCAGC | 4 cut(s) | 120, 601, 622, 648 |
| BseYI | CCCAGC | 1 cut(s) | 423 |
| Bsh1236I | CGCG | 1 cut(s) | 57 |
| BshFI | GGCC | 7 cut(s) | 123, 215, 230, 328, 409, 549, 684 |
| BshNI | GGYRCC | 1 cut(s) | 346 |
| BsiHKAI | GWGCWC | 1 cut(s) | 314 |
| BsiSI | CCGG | 7 cut(s) | 74, 84, 287, 325, 336, 541, 685 |
| BsiWI | CGTACG | 1 cut(s) | 689 |
| BslFI | GGGAC | 2 cut(s) | 359, 696 |
| BslI | CCNNNNNNNGG | 4 cut(s) | 223, 335, 475, 518 |
| BsmAI | GTCTC | 1 cut(s) | 86 |
| BsmFI | GGGAC | 2 cut(s) | 359, 696 |
| BsmI | GAATGC | 1 cut(s) | 541 |
| BsnI | GGCC | 7 cut(s) | 123, 215, 230, 328, 409, 549, 684 |
| Bsp1286I | GDGCHC | 2 cut(s) | 314, 423 |
| Bsp143I | GATC | 4 cut(s) | 160, 521, 665, 732 |
| BspANI | GGCC | 7 cut(s) | 123, 215, 230, 328, 409, 549, 684 |
| BspFNI | CGCG | 1 cut(s) | 57 |
| BspLI | GGNNCC | 3 cut(s) | 348, 375, 550 |
| BspMI | ACCTGC | 3 cut(s) | 214, 361, 514 |
| BspPI | GGATC | 4 cut(s) | 168, 529, 660, 727 |
| BspT107I | GGYRCC | 1 cut(s) | 346 |
| BsrBI | CCGCTC | 1 cut(s) | 471 |
| BsrFI | RCCGGY | 3 cut(s) | 324, 335, 684 |
| BsrI | ACTGG | 5 cut(s) | 18, 38, 159, 356, 582 |
| BssAI | RCCGGY | 3 cut(s) | 324, 335, 684 |
| BssECI | CCNNGG | 4 cut(s) | 72, 82, 210, 512 |
| BssMI | GATC | 4 cut(s) | 160, 521, 665, 732 |
| BssSI | CACGAG | 1 cut(s) | 472 |
| BssT1I | CCWWGG | 1 cut(s) | 512 |
| Bst2BI | CACGAG | 1 cut(s) | 472 |
| Bst2UI | CCWGG | 3 cut(s) | 120, 316, 642 |
| Bst4CI | ACNGT | 1 cut(s) | 721 |
| BstC8I | GCNNGC | 6 cut(s) | 11, 150, 175, 326, 556, 686 |
| BstEII | GGTNACC | 1 cut(s) | 765 |
| BstENI | CCTNNNNNAGG | 1 cut(s) | 516 |
| BstF5I | GGATG | 2 cut(s) | 591, 612 |
| BstFNI | CGCG | 1 cut(s) | 57 |
| BstH2I | RGCGCY | 1 cut(s) | 616 |
| BstHHI | GCGC | 3 cut(s) | 59, 341, 615 |
| BstKTI | GATC | 4 cut(s) | 163, 524, 668, 735 |
| BstMAI | GTCTC | 1 cut(s) | 86 |
| BstMBI | GATC | 4 cut(s) | 160, 521, 665, 732 |
| BstMWI | GCNNNNNNNGC | 7 cut(s) | 54, 120, 287, 325, 376, 555, 560 |
| BstNI | CCWGG | 3 cut(s) | 120, 316, 642 |
| BstPI | GGTNACC | 1 cut(s) | 765 |
| BstSCI | CCNGG | 7 cut(s) | 72, 82, 118, 285, 314, 539, 640 |
| BstSFI | CTRYAG | 1 cut(s) | 670 |
| BstSLI | GKGCMC | 1 cut(s) | 423 |
| BstUI | CGCG | 1 cut(s) | 57 |
| BstV1I | GCAGC | 4 cut(s) | 120, 601, 622, 648 |
| BstX2I | RGATCY | 1 cut(s) | 160 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 721 |
| BstYI | RGATCY | 1 cut(s) | 160 |
| BsuRI | GGCC | 7 cut(s) | 123, 215, 230, 328, 409, 549, 684 |
| BtsCI | GGATG | 2 cut(s) | 591, 612 |
| BtsIMutI | CAGTG | 2 cut(s) | 152, 726 |
| BveI | ACCTGC | 3 cut(s) | 214, 361, 514 |
| Cac8I | GCNNGC | 6 cut(s) | 11, 150, 175, 326, 556, 686 |
| CfoI | GCGC | 3 cut(s) | 59, 341, 615 |
| Cfr10I | RCCGGY | 3 cut(s) | 324, 335, 684 |
| Cfr13I | GGNCC | 4 cut(s) | 214, 373, 548, 649 |
| CseI | GACGC | 1 cut(s) | 569 |
| Csp6I | GTAC | 3 cut(s) | 333, 347, 690 |
| CviAII | CATG | 2 cut(s) | 440, 795 |
| CviQI | GTAC | 3 cut(s) | 333, 347, 690 |
| DpnI | GATC | 4 cut(s) | 162, 523, 667, 734 |
| DpnII | GATC | 4 cut(s) | 160, 521, 665, 732 |
| EaeI | YGGCCR | 1 cut(s) | 228 |
| EciI | GGCGGA | 2 cut(s) | 12, 173 |
| Eco130I | CCWWGG | 1 cut(s) | 512 |
| Eco147I | AGGCCT | 1 cut(s) | 409 |
| Eco47I | GGWCC | 2 cut(s) | 373, 649 |
| Eco57I | CTGAAG | 1 cut(s) | 23 |
| Eco91I | GGTNACC | 1 cut(s) | 765 |
| EcoNI | CCTNNNNNAGG | 1 cut(s) | 516 |
| EcoO109I | RGGNCCY | 2 cut(s) | 373, 548 |
| EcoO65I | GGTNACC | 1 cut(s) | 765 |
| EcoRII | CCWGG | 3 cut(s) | 118, 314, 640 |
| EcoT14I | CCWWGG | 1 cut(s) | 512 |
| ErhI | CCWWGG | 1 cut(s) | 512 |
| FaeI | CATG | 2 cut(s) | 443, 798 |
| FaiI | YATR | 8 cut(s) | 142, 144, 366, 368, 441, 746, 748, 796 |
| FaqI | GGGAC | 2 cut(s) | 359, 696 |
| FatI | CATG | 2 cut(s) | 439, 794 |
| FauI | CCCGC | 2 cut(s) | 384, 559 |
| FauNDI | CATATG | 2 cut(s) | 366, 746 |
| Fnu4HI | GCNGC | 5 cut(s) | 109, 112, 590, 611, 662 |
| FokI | GGATG | 2 cut(s) | 598, 619 |
| Fsp4HI | GCNGC | 5 cut(s) | 109, 112, 590, 611, 662 |
| FspBI | CTAG | 3 cut(s) | 330, 480, 513 |
| GlaI | GCGC | 3 cut(s) | 58, 340, 614 |
| GluI | GCNGC | 5 cut(s) | 109, 112, 590, 611, 662 |
| GsaI | CCCAGC | 1 cut(s) | 427 |
| GsuI | CTGGAG | 1 cut(s) | 290 |
| HaeII | RGCGCY | 1 cut(s) | 616 |
| HaeIII | GGCC | 7 cut(s) | 123, 215, 230, 328, 409, 549, 684 |
| HapII | CCGG | 7 cut(s) | 74, 84, 287, 325, 336, 541, 685 |
| HgaI | GACGC | 1 cut(s) | 569 |
| HhaI | GCGC | 3 cut(s) | 59, 341, 615 |
| Hin1II | CATG | 2 cut(s) | 443, 798 |
| Hin6I | GCGC | 3 cut(s) | 57, 339, 613 |
| HinP1I | GCGC | 3 cut(s) | 57, 339, 613 |
| HinfI | GANTC | 3 cut(s) | 449, 637, 699 |
| HpaII | CCGG | 7 cut(s) | 74, 84, 287, 325, 336, 541, 685 |
| HphI | GGTGA | 4 cut(s) | 101, 124, 648, 759 |
| Hpy166II | GTNNAC | 1 cut(s) | 79 |
| Hpy188III | TCNNGA | 1 cut(s) | 759 |
| Hpy8I | GTNNAC | 1 cut(s) | 79 |
| Hpy99I | CGWCG | 1 cut(s) | 563 |
| HpyAV | CCTTC | 4 cut(s) | 396, 747, 756, 765 |
| HpyCH4III | ACNGT | 1 cut(s) | 721 |
| HpyCH4IV | ACGT | 1 cut(s) | 244 |
| HpyCH4V | TGCA | 5 cut(s) | 198, 364, 370, 589, 610 |
| HpyF10VI | GCNNNNNNNGC | 7 cut(s) | 54, 120, 287, 325, 376, 555, 560 |
| HpySE526I | ACGT | 1 cut(s) | 244 |
| Hsp92II | CATG | 2 cut(s) | 443, 798 |
| HspAI | GCGC | 3 cut(s) | 57, 339, 613 |
| KpnI | GGTACC | 1 cut(s) | 350 |
| KroI | GCCGGC | 2 cut(s) | 324, 684 |
| KroNI | GCCGGC | 2 cut(s) | 326, 686 |
| Kzo9I | GATC | 4 cut(s) | 160, 521, 665, 732 |
| LmnI | GCTCC | 3 cut(s) | 294, 309, 436 |
| Lsp1109I | GCAGC | 4 cut(s) | 120, 601, 622, 648 |
| LweI | GCATC | 3 cut(s) | 57, 576, 597 |
| MaeI | CTAG | 3 cut(s) | 330, 480, 513 |
| MaeII | ACGT | 1 cut(s) | 244 |
| MaeIII | GTNAC | 3 cut(s) | 240, 456, 765 |
| MalI | GATC | 4 cut(s) | 162, 523, 667, 734 |
| MbiI | CCGCTC | 1 cut(s) | 471 |
| MboI | GATC | 4 cut(s) | 160, 521, 665, 732 |
| MboII | GAAGA | 2 cut(s) | 16, 155 |
| MflI | RGATCY | 1 cut(s) | 160 |
| MhlI | GDGCHC | 2 cut(s) | 314, 423 |
| MlsI | TGGCCA | 1 cut(s) | 230 |
| MluCI | AATT | 1 cut(s) | 617 |
| MluNI | TGGCCA | 1 cut(s) | 230 |
| MlyI | GAGTC | 2 cut(s) | 646, 693 |
| MmeI | TCCRAC | 2 cut(s) | 414, 576 |
| MnlI | CCTC | 9 cut(s) | 140, 145, 205, 265, 399, 420, 436, 487, 512 |
| Mox20I | TGGCCA | 1 cut(s) | 230 |
| MroNI | GCCGGC | 2 cut(s) | 324, 684 |
| MscI | TGGCCA | 1 cut(s) | 230 |
| MslI | CAYNNNNRTG | 2 cut(s) | 236, 719 |
| Msp20I | TGGCCA | 1 cut(s) | 230 |
| MspA1I | CMGCKG | 4 cut(s) | 379, 532, 554, 661 |
| MspI | CCGG | 7 cut(s) | 74, 84, 287, 325, 336, 541, 685 |
| MspR9I | CCNGG | 7 cut(s) | 74, 84, 120, 287, 316, 541, 642 |
| Mva1269I | GAATGC | 1 cut(s) | 541 |
| MvaI | CCWGG | 3 cut(s) | 120, 316, 642 |
| MvnI | CGCG | 1 cut(s) | 57 |
| MwoI | GCNNNNNNNGC | 7 cut(s) | 54, 120, 287, 325, 376, 555, 560 |
| NaeI | GCCGGC | 2 cut(s) | 326, 686 |
| NciI | CCSGG | 4 cut(s) | 74, 84, 287, 541 |
| NdeI | CATATG | 2 cut(s) | 366, 746 |
| NdeII | GATC | 4 cut(s) | 160, 521, 665, 732 |
| NgoMIV | GCCGGC | 2 cut(s) | 324, 684 |
| NlaIII | CATG | 2 cut(s) | 443, 798 |
| NlaIV | GGNNCC | 3 cut(s) | 348, 375, 550 |
| NmeAIII | GCCGAG | 1 cut(s) | 235 |
| NmuCI | GTSAC | 2 cut(s) | 240, 765 |
| OliI | CACNNNNGTG | 1 cut(s) | 236 |
| PaqCI | CACCTGC | 2 cut(s) | 214, 514 |
| PceI | AGGCCT | 1 cut(s) | 409 |
| PctI | GAATGC | 1 cut(s) | 541 |
| PdiI | GCCGGC | 2 cut(s) | 326, 686 |
| PfeI | GAWTC | 1 cut(s) | 449 |
| Pfl23II | CGTACG | 1 cut(s) | 689 |
| PkrI | GCNGC | 5 cut(s) | 110, 113, 591, 612, 663 |
| PleI | GAGTC | 2 cut(s) | 645, 693 |
| PpsI | GAGTC | 2 cut(s) | 645, 693 |
| PpuMI | RGGWCCY | 1 cut(s) | 373 |
| Psp5II | RGGWCCY | 1 cut(s) | 373 |
| Psp6I | CCWGG | 3 cut(s) | 118, 314, 640 |
| PspEI | GGTNACC | 1 cut(s) | 765 |
| PspFI | CCCAGC | 1 cut(s) | 423 |
| PspGI | CCWGG | 3 cut(s) | 118, 314, 640 |
| PspLI | CGTACG | 1 cut(s) | 689 |
| PspN4I | GGNNCC | 3 cut(s) | 348, 375, 550 |
| PspPI | GGNCC | 4 cut(s) | 214, 373, 548, 649 |
| PspPPI | RGGWCCY | 1 cut(s) | 373 |
| PsuI | RGATCY | 1 cut(s) | 160 |
| RsaI | GTAC | 3 cut(s) | 334, 348, 691 |
| RsaNI | GTAC | 3 cut(s) | 333, 347, 690 |
| RseI | CAYNNNNRTG | 2 cut(s) | 236, 719 |
| SatI | GCNGC | 5 cut(s) | 109, 112, 590, 611, 662 |
| Sau3AI | GATC | 4 cut(s) | 160, 521, 665, 732 |
| Sau96I | GGNCC | 4 cut(s) | 214, 373, 548, 649 |
| SchI | GAGTC | 2 cut(s) | 646, 693 |
| ScrFI | CCNGG | 7 cut(s) | 74, 84, 120, 287, 316, 541, 642 |
| SduI | GDGCHC | 2 cut(s) | 314, 423 |
| SfaNI | GCATC | 3 cut(s) | 57, 576, 597 |
| SfcI | CTRYAG | 1 cut(s) | 670 |
| SinI | GGWCC | 2 cut(s) | 373, 649 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 236, 719 |
| Sse9I | AATT | 1 cut(s) | 617 |
| SseBI | AGGCCT | 1 cut(s) | 409 |
| SspMI | CTAG | 3 cut(s) | 330, 480, 513 |
| StuI | AGGCCT | 1 cut(s) | 409 |
| StyD4I | CCNGG | 7 cut(s) | 72, 82, 118, 285, 314, 539, 640 |
| StyI | CCWWGG | 1 cut(s) | 512 |
| TaaI | ACNGT | 1 cut(s) | 721 |
| TaiI | ACGT | 1 cut(s) | 247 |
| TaqI | TCGA | 1 cut(s) | 635 |
| TasI | AATT | 1 cut(s) | 617 |
| TauI | GCSGC | 1 cut(s) | 114 |
| TfiI | GAWTC | 1 cut(s) | 449 |
| TscAI | CASTG | 2 cut(s) | 159, 726 |
| TseFI | GTSAC | 2 cut(s) | 240, 765 |
| TseI | GCWGC | 4 cut(s) | 108, 589, 610, 661 |
| Tsp45I | GTSAC | 2 cut(s) | 240, 765 |
| TspDTI | ATGAA | 1 cut(s) | 267 |
| TspGWI | ACGGA | 2 cut(s) | 307, 322 |
| TspRI | CASTG | 2 cut(s) | 159, 726 |
| VpaK11BI | GGWCC | 2 cut(s) | 373, 649 |
| XagI | CCTNNNNNAGG | 1 cut(s) | 516 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 574 |
| XmaJI | CCTAGG | 1 cut(s) | 512 |
| XspI | CTAG | 3 cut(s) | 330, 480, 513 |