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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 310 |
| AccB1I | GGYRCC | 2 cut(s) | 310, 554 |
| AccBSI | CCGCTC | 1 cut(s) | 454 |
| AccI | GTMKAC | 1 cut(s) | 282 |
| AccII | CGCG | 3 cut(s) | 270, 584, 667 |
| AclWI | GGATC | 2 cut(s) | 607, 620 |
| AcoI | YGGCCR | 1 cut(s) | 192 |
| AcsI | RAATTY | 1 cut(s) | 209 |
| AcyI | GRCGYC | 2 cut(s) | 555, 735 |
| AfaI | GTAC | 2 cut(s) | 312, 636 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 187, 299, 445, 473, 548 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 214, 246 |
| AluBI | AGCT | 2 cut(s) | 38, 96 |
| AluI | AGCT | 2 cut(s) | 38, 96 |
| Alw21I | GWGCWC | 2 cut(s) | 153, 278 |
| AlwI | GGATC | 2 cut(s) | 607, 620 |
| Ama87I | CYCGRG | 2 cut(s) | 39, 458 |
| AoxI | GGCC | 5 cut(s) | 178, 192, 338, 455, 477 |
| ApaI | GGGCCC | 2 cut(s) | 342, 481 |
| ApoI | RAATTY | 1 cut(s) | 209 |
| Asp718I | GGTACC | 1 cut(s) | 310 |
| AspLEI | GCGC | 2 cut(s) | 255, 557 |
| AspS9I | GGNCC | 9 cut(s) | 52, 127, 179, 317, 338, 339, 456, 477, 478 |
| AsuC2I | CCSGG | 6 cut(s) | 49, 250, 315, 471, 507, 543 |
| AsuHPI | GGTGA | 4 cut(s) | 209, 594, 619, 703 |
| AvaI | CYCGRG | 2 cut(s) | 39, 458 |
| AvaII | GGWCC | 3 cut(s) | 52, 127, 317 |
| BaeGI | GKGCMC | 2 cut(s) | 342, 481 |
| BalI | TGGCCA | 1 cut(s) | 194 |
| BamHI | GGATCC | 1 cut(s) | 612 |
| BanI | GGYRCC | 2 cut(s) | 310, 554 |
| BanII | GRGCYC | 2 cut(s) | 342, 481 |
| BauI | CACGAG | 1 cut(s) | 11 |
| Bbv12I | GWGCWC | 2 cut(s) | 153, 278 |
| BccI | CCATC | 2 cut(s) | 329, 632 |
| BclI | TGATCA | 1 cut(s) | 214 |
| BcnI | CCSGG | 6 cut(s) | 49, 250, 315, 471, 507, 543 |
| BfaI | CTAG | 3 cut(s) | 419, 525, 608 |
| BfoI | RGCGCY | 1 cut(s) | 558 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 250 |
| BisI | GCNGC | 4 cut(s) | 118, 455, 553, 668 |
| BlsI | GCNGC | 4 cut(s) | 119, 456, 554, 669 |
| Bme1390I | CCNGG | 6 cut(s) | 49, 250, 315, 471, 507, 543 |
| Bme18I | GGWCC | 3 cut(s) | 52, 127, 317 |
| BmeT110I | CYCGRG | 2 cut(s) | 39, 458 |
| BmgT120I | GGNCC | 9 cut(s) | 52, 127, 179, 317, 338, 339, 456, 477, 478 |
| BmiI | GGNNCC | 9 cut(s) | 53, 312, 318, 340, 465, 478, 479, 556, 614 |
| BmrFI | CCNGG | 6 cut(s) | 49, 250, 315, 471, 507, 543 |
| BmsI | GCATC | 1 cut(s) | 505 |
| BpuMI | CCSGG | 6 cut(s) | 49, 250, 315, 471, 507, 543 |
| BsaHI | GRCGYC | 2 cut(s) | 555, 735 |
| BsaJI | CCNNGG | 4 cut(s) | 48, 425, 459, 481 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 366, 396 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 187, 299, 445, 473, 548 |
| Bse118I | RCCGGY | 1 cut(s) | 629 |
| Bse3DI | GCAATG | 1 cut(s) | 120 |
| BseDI | CCNNGG | 4 cut(s) | 48, 425, 459, 481 |
| BseGI | GGATG | 2 cut(s) | 340, 520 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 187, 299, 445, 473, 548 |
| BseMI | GCAATG | 1 cut(s) | 120 |
| BseRI | GAGGAG | 1 cut(s) | 561 |
| BseSI | GKGCMC | 2 cut(s) | 342, 481 |
| Bsh1236I | CGCG | 3 cut(s) | 270, 584, 667 |
| BshFI | GGCC | 5 cut(s) | 180, 194, 340, 457, 479 |
| BshNI | GGYRCC | 2 cut(s) | 310, 554 |
| BsiHKAI | GWGCWC | 2 cut(s) | 153, 278 |
| BsiHKCI | CYCGRG | 2 cut(s) | 39, 458 |
| BsiSI | CCGG | 9 cut(s) | 48, 250, 314, 446, 470, 507, 542, 630, 768 |
| BsiWI | CGTACG | 1 cut(s) | 634 |
| BslFI | GGGAC | 3 cut(s) | 52, 65, 330 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 187, 299, 445, 473, 548 |
| BsmFI | GGGAC | 3 cut(s) | 52, 65, 330 |
| BsnI | GGCC | 5 cut(s) | 180, 194, 340, 457, 479 |
| BsoBI | CYCGRG | 2 cut(s) | 39, 458 |
| Bsp120I | GGGCCC | 2 cut(s) | 338, 477 |
| Bsp1286I | GDGCHC | 4 cut(s) | 153, 278, 342, 481 |
| Bsp143I | GATC | 3 cut(s) | 90, 214, 612 |
| BspANI | GGCC | 5 cut(s) | 180, 194, 340, 457, 479 |
| BspFNI | CGCG | 3 cut(s) | 270, 584, 667 |
| BspHI | TCATGA | 1 cut(s) | 324 |
| BspLI | GGNNCC | 9 cut(s) | 53, 312, 318, 340, 465, 478, 479, 556, 614 |
| BspPI | GGATC | 2 cut(s) | 607, 620 |
| BspT107I | GGYRCC | 2 cut(s) | 310, 554 |
| BsrBI | CCGCTC | 1 cut(s) | 454 |
| BsrDI | GCAATG | 1 cut(s) | 120 |
| BsrFI | RCCGGY | 1 cut(s) | 629 |
| BssAI | RCCGGY | 1 cut(s) | 629 |
| BssECI | CCNNGG | 4 cut(s) | 48, 425, 459, 481 |
| BssMI | GATC | 3 cut(s) | 90, 214, 612 |
| BssNAI | GTATAC | 1 cut(s) | 283 |
| BssNI | GRCGYC | 2 cut(s) | 555, 735 |
| BssSI | CACGAG | 1 cut(s) | 11 |
| Bst1107I | GTATAC | 1 cut(s) | 283 |
| Bst2BI | CACGAG | 1 cut(s) | 11 |
| Bst4CI | ACNGT | 2 cut(s) | 280, 774 |
| BstACI | GRCGYC | 2 cut(s) | 555, 735 |
| BstC8I | GCNNGC | 2 cut(s) | 262, 621 |
| BstDSI | CCRYGG | 1 cut(s) | 481 |
| BstF5I | GGATG | 2 cut(s) | 340, 520 |
| BstFNI | CGCG | 3 cut(s) | 270, 584, 667 |
| BstH2I | RGCGCY | 1 cut(s) | 558 |
| BstHHI | GCGC | 2 cut(s) | 255, 557 |
| BstKTI | GATC | 3 cut(s) | 93, 217, 615 |
| BstMBI | GATC | 3 cut(s) | 90, 214, 612 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 44, 235, 250, 261, 415 |
| BstNSI | RCATGY | 1 cut(s) | 623 |
| BstSCI | CCNGG | 6 cut(s) | 47, 248, 313, 469, 505, 541 |
| BstSLI | GKGCMC | 2 cut(s) | 342, 481 |
| BstUI | CGCG | 3 cut(s) | 270, 584, 667 |
| BstX2I | RGATCY | 1 cut(s) | 612 |
| BstYI | RGATCY | 1 cut(s) | 612 |
| BstZ17I | GTATAC | 1 cut(s) | 283 |
| BsuRI | GGCC | 5 cut(s) | 180, 194, 340, 457, 479 |
| BtgI | CCRYGG | 1 cut(s) | 481 |
| BtsCI | GGATG | 2 cut(s) | 340, 520 |
| Cac8I | GCNNGC | 2 cut(s) | 262, 621 |
| CciI | TCATGA | 1 cut(s) | 324 |
| CfoI | GCGC | 2 cut(s) | 255, 557 |
| Cfr10I | RCCGGY | 1 cut(s) | 629 |
| Cfr13I | GGNCC | 9 cut(s) | 52, 127, 179, 317, 338, 339, 456, 477, 478 |
| CseI | GACGC | 3 cut(s) | 498, 573, 743 |
| Csp6I | GTAC | 2 cut(s) | 311, 635 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 171, 206 |
| CviAII | CATG | 5 cut(s) | 82, 196, 325, 620, 671 |
| CviQI | GTAC | 2 cut(s) | 311, 635 |
| DinI | GGCGCC | 1 cut(s) | 556 |
| DpnI | GATC | 3 cut(s) | 92, 216, 614 |
| DpnII | GATC | 3 cut(s) | 90, 214, 612 |
| EaeI | YGGCCR | 1 cut(s) | 192 |
| EciI | GGCGGA | 3 cut(s) | 189, 318, 464 |
| Eco24I | GRGCYC | 2 cut(s) | 342, 481 |
| Eco47I | GGWCC | 3 cut(s) | 52, 127, 317 |
| Eco88I | CYCGRG | 2 cut(s) | 39, 458 |
| EcoO109I | RGGNCCY | 1 cut(s) | 477 |
| EcoT38I | GRGCYC | 2 cut(s) | 342, 481 |
| EgeI | GGCGCC | 1 cut(s) | 556 |
| EheI | GGCGCC | 1 cut(s) | 556 |
| FaeI | CATG | 5 cut(s) | 85, 199, 328, 623, 674 |
| FaqI | GGGAC | 3 cut(s) | 52, 65, 330 |
| FatI | CATG | 5 cut(s) | 81, 195, 324, 619, 670 |
| FauI | CCCGC | 4 cut(s) | 263, 467, 567, 624 |
| FauNDI | CATATG | 1 cut(s) | 691 |
| FbaI | TGATCA | 1 cut(s) | 214 |
| FblI | GTMKAC | 1 cut(s) | 282 |
| Fnu4HI | GCNGC | 4 cut(s) | 118, 455, 553, 668 |
| FokI | GGATG | 2 cut(s) | 347, 527 |
| FriOI | GRGCYC | 2 cut(s) | 342, 481 |
| Fsp4HI | GCNGC | 4 cut(s) | 118, 455, 553, 668 |
| FspBI | CTAG | 3 cut(s) | 419, 525, 608 |
| GlaI | GCGC | 2 cut(s) | 254, 556 |
| GluI | GCNGC | 4 cut(s) | 118, 455, 553, 668 |
| HaeII | RGCGCY | 1 cut(s) | 558 |
| HaeIII | GGCC | 5 cut(s) | 180, 194, 340, 457, 479 |
| HapII | CCGG | 9 cut(s) | 48, 250, 314, 446, 470, 507, 542, 630, 768 |
| HgaI | GACGC | 3 cut(s) | 498, 573, 743 |
| HhaI | GCGC | 2 cut(s) | 255, 557 |
| Hin1I | GRCGYC | 2 cut(s) | 555, 735 |
| Hin1II | CATG | 5 cut(s) | 85, 199, 328, 623, 674 |
| Hin6I | GCGC | 2 cut(s) | 253, 555 |
| HinP1I | GCGC | 2 cut(s) | 253, 555 |
| HpaII | CCGG | 9 cut(s) | 48, 250, 314, 446, 470, 507, 542, 630, 768 |
| HphI | GGTGA | 4 cut(s) | 209, 594, 619, 703 |
| Hpy166II | GTNNAC | 1 cut(s) | 283 |
| Hpy188III | TCNNGA | 2 cut(s) | 41, 325 |
| Hpy8I | GTNNAC | 1 cut(s) | 283 |
| Hpy99I | CGWCG | 1 cut(s) | 492 |
| HpyAV | CCTTC | 4 cut(s) | 109, 140, 219, 322 |
| HpyCH4III | ACNGT | 2 cut(s) | 280, 774 |
| HpyCH4V | TGCA | 1 cut(s) | 518 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 44, 235, 250, 261, 415 |
| Hsp92I | GRCGYC | 2 cut(s) | 555, 735 |
| Hsp92II | CATG | 5 cut(s) | 85, 199, 328, 623, 674 |
| HspAI | GCGC | 2 cut(s) | 253, 555 |
| KasI | GGCGCC | 1 cut(s) | 554 |
| KpnI | GGTACC | 1 cut(s) | 314 |
| Ksp22I | TGATCA | 1 cut(s) | 214 |
| Kzo9I | GATC | 3 cut(s) | 90, 214, 612 |
| LmnI | GCTCC | 4 cut(s) | 101, 273, 368, 451 |
| LweI | GCATC | 1 cut(s) | 505 |
| MaeI | CTAG | 3 cut(s) | 419, 525, 608 |
| MaeIII | GTNAC | 2 cut(s) | 382, 656 |
| MalI | GATC | 3 cut(s) | 92, 216, 614 |
| MbiI | CCGCTC | 1 cut(s) | 454 |
| MboI | GATC | 3 cut(s) | 90, 214, 612 |
| MboII | GAAGA | 4 cut(s) | 97, 100, 234, 571 |
| MflI | RGATCY | 1 cut(s) | 612 |
| MhlI | GDGCHC | 4 cut(s) | 153, 278, 342, 481 |
| MlsI | TGGCCA | 1 cut(s) | 194 |
| MluCI | AATT | 1 cut(s) | 209 |
| MluNI | TGGCCA | 1 cut(s) | 194 |
| Mly113I | GGCGCC | 1 cut(s) | 555 |
| MmeI | TCCRAC | 2 cut(s) | 212, 346 |
| Mox20I | TGGCCA | 1 cut(s) | 194 |
| MscI | TGGCCA | 1 cut(s) | 194 |
| MseI | TTAA | 2 cut(s) | 501, 702 |
| MslI | CAYNNNNRTG | 1 cut(s) | 200 |
| Msp20I | TGGCCA | 1 cut(s) | 194 |
| MspI | CCGG | 9 cut(s) | 48, 250, 314, 446, 470, 507, 542, 630, 768 |
| MspR9I | CCNGG | 6 cut(s) | 49, 250, 315, 471, 507, 543 |
| MvnI | CGCG | 3 cut(s) | 270, 584, 667 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 44, 235, 250, 261, 415 |
| NarI | GGCGCC | 1 cut(s) | 555 |
| NciI | CCSGG | 6 cut(s) | 49, 250, 315, 471, 507, 543 |
| NdeI | CATATG | 1 cut(s) | 691 |
| NdeII | GATC | 3 cut(s) | 90, 214, 612 |
| NlaIII | CATG | 5 cut(s) | 85, 199, 328, 623, 674 |
| NlaIV | GGNNCC | 9 cut(s) | 53, 312, 318, 340, 465, 478, 479, 556, 614 |
| NmuCI | GTSAC | 2 cut(s) | 382, 656 |
| NspI | RCATGY | 1 cut(s) | 623 |
| PaeI | GCATGC | 1 cut(s) | 623 |
| PagI | TCATGA | 1 cut(s) | 324 |
| Pfl23II | CGTACG | 1 cut(s) | 634 |
| PfoI | TCCNGGA | 2 cut(s) | 505, 541 |
| PkrI | GCNGC | 4 cut(s) | 119, 456, 554, 669 |
| PluTI | GGCGCC | 1 cut(s) | 558 |
| PspLI | CGTACG | 1 cut(s) | 634 |
| PspN4I | GGNNCC | 9 cut(s) | 53, 312, 318, 340, 465, 478, 479, 556, 614 |
| PspOMI | GGGCCC | 2 cut(s) | 338, 477 |
| PspPI | GGNCC | 9 cut(s) | 52, 127, 179, 317, 338, 339, 456, 477, 478 |
| PsuI | RGATCY | 1 cut(s) | 612 |
| RsaI | GTAC | 2 cut(s) | 312, 636 |
| RsaNI | GTAC | 2 cut(s) | 311, 635 |
| RseI | CAYNNNNRTG | 1 cut(s) | 200 |
| SaqAI | TTAA | 2 cut(s) | 501, 702 |
| SatI | GCNGC | 4 cut(s) | 118, 455, 553, 668 |
| Sau3AI | GATC | 3 cut(s) | 90, 214, 612 |
| Sau96I | GGNCC | 9 cut(s) | 52, 127, 179, 317, 338, 339, 456, 477, 478 |
| ScrFI | CCNGG | 6 cut(s) | 49, 250, 315, 471, 507, 543 |
| SduI | GDGCHC | 4 cut(s) | 153, 278, 342, 481 |
| SfaNI | GCATC | 1 cut(s) | 505 |
| SfoI | GGCGCC | 1 cut(s) | 556 |
| SgrAI | CRCCGGYG | 1 cut(s) | 629 |
| SinI | GGWCC | 3 cut(s) | 52, 127, 317 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 200 |
| SphI | GCATGC | 1 cut(s) | 623 |
| Sse9I | AATT | 1 cut(s) | 209 |
| SspDI | GGCGCC | 1 cut(s) | 554 |
| SspMI | CTAG | 3 cut(s) | 419, 525, 608 |
| StyD4I | CCNGG | 6 cut(s) | 47, 248, 313, 469, 505, 541 |
| TaaI | ACNGT | 2 cut(s) | 280, 774 |
| TaqI | TCGA | 2 cut(s) | 33, 75 |
| TaqII | GACCGA | 2 cut(s) | 69, 690 |
| TasI | AATT | 1 cut(s) | 209 |
| TauI | GCSGC | 4 cut(s) | 120, 457, 555, 670 |
| Tru1I | TTAA | 2 cut(s) | 501, 702 |
| Tru9I | TTAA | 2 cut(s) | 501, 702 |
| TseFI | GTSAC | 2 cut(s) | 382, 656 |
| Tsp45I | GTSAC | 2 cut(s) | 382, 656 |
| TspDTI | ATGAA | 2 cut(s) | 98, 341 |
| TspGWI | ACGGA | 1 cut(s) | 577 |
| VpaK11BI | GGWCC | 3 cut(s) | 52, 127, 317 |
| XapI | RAATTY | 1 cut(s) | 209 |
| XceI | RCATGY | 1 cut(s) | 623 |
| XmiI | GTMKAC | 1 cut(s) | 282 |
| XspI | CTAG | 3 cut(s) | 419, 525, 608 |