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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc65I | GGTACC | 1 cut(s) | 28 |
| AccB1I | GGYRCC | 1 cut(s) | 28 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 162 |
| AccII | CGCG | 3 cut(s) | 261, 356, 589 |
| AclWI | GGATC | 1 cut(s) | 102 |
| AcoI | YGGCCR | 2 cut(s) | 357, 510 |
| AcsI | RAATTY | 1 cut(s) | 527 |
| AcuI | CTGAAG | 2 cut(s) | 191, 560 |
| AcyI | GRCGYC | 1 cut(s) | 275 |
| AfaI | GTAC | 2 cut(s) | 30, 479 |
| AfiI | CCNNNNNNNGG | 5 cut(s) | 91, 162, 230, 451, 616 |
| AgsI | TTSAA | 1 cut(s) | 400 |
| AjnI | CCWGG | 1 cut(s) | 23 |
| AluBI | AGCT | 4 cut(s) | 153, 162, 374, 430 |
| AluI | AGCT | 4 cut(s) | 153, 162, 374, 430 |
| AlwI | GGATC | 1 cut(s) | 102 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 162 |
| Ama87I | CYCGRG | 1 cut(s) | 84 |
| AoxI | GGCC | 3 cut(s) | 357, 495, 510 |
| ApeKI | GCWGC | 1 cut(s) | 209 |
| ApoI | RAATTY | 1 cut(s) | 527 |
| Asp718I | GGTACC | 1 cut(s) | 28 |
| AspLEI | GCGC | 2 cut(s) | 130, 550 |
| AspS9I | GGNCC | 3 cut(s) | 249, 442, 496 |
| AsuC2I | CCSGG | 1 cut(s) | 92 |
| AsuHPI | GGTGA | 2 cut(s) | 89, 100 |
| AsuNHI | GCTAGC | 1 cut(s) | 41 |
| AvaI | CYCGRG | 1 cut(s) | 84 |
| AvaII | GGWCC | 2 cut(s) | 249, 442 |
| BalI | TGGCCA | 1 cut(s) | 512 |
| BanI | GGYRCC | 1 cut(s) | 28 |
| BanII | GRGCYC | 1 cut(s) | 91 |
| BbvCI | CCTCAGC | 1 cut(s) | 309 |
| BbvI | GCAGC | 1 cut(s) | 221 |
| BccI | CCATC | 2 cut(s) | 348, 577 |
| BciT130I | CCWGG | 1 cut(s) | 25 |
| BcnI | CCSGG | 1 cut(s) | 92 |
| BfaI | CTAG | 2 cut(s) | 42, 597 |
| BfoI | RGCGCY | 1 cut(s) | 551 |
| BglI | GCCNNNNNGGC | 1 cut(s) | 74 |
| BisI | GCNGC | 3 cut(s) | 210, 357, 417 |
| BlsI | GCNGC | 3 cut(s) | 211, 358, 418 |
| Bme1390I | CCNGG | 2 cut(s) | 25, 92 |
| Bme18I | GGWCC | 2 cut(s) | 249, 442 |
| BmeT110I | CYCGRG | 1 cut(s) | 84 |
| BmgT120I | GGNCC | 3 cut(s) | 249, 442, 496 |
| BmiI | GGNNCC | 1 cut(s) | 30 |
| BmrFI | CCNGG | 2 cut(s) | 25, 92 |
| BmsI | GCATC | 1 cut(s) | 243 |
| BmtI | GCTAGC | 1 cut(s) | 45 |
| BplI | GAGNNNNNCTC | 1 cut(s) | 27 |
| BpmI | CTGGAG | 2 cut(s) | 139, 572 |
| Bpu10I | CCTNAGC | 1 cut(s) | 309 |
| BpuMI | CCSGG | 1 cut(s) | 92 |
| BsaHI | GRCGYC | 1 cut(s) | 275 |
| BsaJI | CCNNGG | 3 cut(s) | 24, 90, 354 |
| Bsc4I | CCNNNNNNNGG | 5 cut(s) | 91, 162, 230, 451, 616 |
| Bse118I | RCCGGY | 1 cut(s) | 616 |
| Bse1I | ACTGG | 1 cut(s) | 441 |
| BseBI | CCWGG | 1 cut(s) | 25 |
| BseDI | CCNNGG | 3 cut(s) | 24, 90, 354 |
| BseGI | GGATG | 1 cut(s) | 340 |
| BseLI | CCNNNNNNNGG | 5 cut(s) | 91, 162, 230, 451, 616 |
| BseMII | CTCAG | 1 cut(s) | 300 |
| BseNI | ACTGG | 1 cut(s) | 441 |
| BseRI | GAGGAG | 1 cut(s) | 326 |
| BseXI | GCAGC | 1 cut(s) | 221 |
| BseYI | CCCAGC | 2 cut(s) | 162, 370 |
| Bsh1236I | CGCG | 3 cut(s) | 261, 356, 589 |
| BshFI | GGCC | 3 cut(s) | 359, 497, 512 |
| BshNI | GGYRCC | 1 cut(s) | 28 |
| BsiHKCI | CYCGRG | 1 cut(s) | 84 |
| BsiSI | CCGG | 2 cut(s) | 92, 617 |
| BslFI | GGGAC | 1 cut(s) | 168 |
| BslI | CCNNNNNNNGG | 5 cut(s) | 91, 162, 230, 451, 616 |
| BsmFI | GGGAC | 1 cut(s) | 168 |
| BsnI | GGCC | 3 cut(s) | 359, 497, 512 |
| BsoBI | CYCGRG | 1 cut(s) | 84 |
| Bsp1286I | GDGCHC | 1 cut(s) | 91 |
| Bsp143I | GATC | 3 cut(s) | 94, 105, 322 |
| BspANI | GGCC | 3 cut(s) | 359, 497, 512 |
| BspCNI | CTCAG | 1 cut(s) | 301 |
| BspFNI | CGCG | 3 cut(s) | 261, 356, 589 |
| BspLI | GGNNCC | 1 cut(s) | 30 |
| BspOI | GCTAGC | 1 cut(s) | 45 |
| BspPI | GGATC | 1 cut(s) | 102 |
| BspT107I | GGYRCC | 1 cut(s) | 28 |
| BsrFI | RCCGGY | 1 cut(s) | 616 |
| BsrI | ACTGG | 1 cut(s) | 441 |
| BssAI | RCCGGY | 1 cut(s) | 616 |
| BssECI | CCNNGG | 3 cut(s) | 24, 90, 354 |
| BssMI | GATC | 3 cut(s) | 94, 105, 322 |
| BssNI | GRCGYC | 1 cut(s) | 275 |
| Bst2UI | CCWGG | 1 cut(s) | 25 |
| Bst4CI | ACNGT | 1 cut(s) | 389 |
| Bst6I | CTCTTC | 3 cut(s) | 197, 220, 237 |
| BstACI | GRCGYC | 1 cut(s) | 275 |
| BstC8I | GCNNGC | 7 cut(s) | 43, 75, 318, 361, 432, 470, 581 |
| BstDEI | CTNAG | 1 cut(s) | 309 |
| BstDSI | CCRYGG | 1 cut(s) | 354 |
| BstENI | CCTNNNNNAGG | 2 cut(s) | 228, 449 |
| BstF5I | GGATG | 1 cut(s) | 340 |
| BstFNI | CGCG | 3 cut(s) | 261, 356, 589 |
| BstH2I | RGCGCY | 1 cut(s) | 551 |
| BstHHI | GCGC | 2 cut(s) | 130, 550 |
| BstKTI | GATC | 3 cut(s) | 97, 108, 325 |
| BstMBI | GATC | 3 cut(s) | 94, 105, 322 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 74, 125, 159, 554, 580 |
| BstNI | CCWGG | 1 cut(s) | 25 |
| BstSCI | CCNGG | 2 cut(s) | 23, 90 |
| BstUI | CGCG | 3 cut(s) | 261, 356, 589 |
| BstV1I | GCAGC | 1 cut(s) | 221 |
| BsuRI | GGCC | 3 cut(s) | 359, 497, 512 |
| BtgI | CCRYGG | 1 cut(s) | 354 |
| BtgZI | GCGATG | 1 cut(s) | 555 |
| BtsCI | GGATG | 1 cut(s) | 340 |
| BtsIMutI | CAGTG | 1 cut(s) | 434 |
| Cac8I | GCNNGC | 7 cut(s) | 43, 75, 318, 361, 432, 470, 581 |
| CaiI | CAGNNNCTG | 1 cut(s) | 162 |
| CfoI | GCGC | 2 cut(s) | 130, 550 |
| Cfr10I | RCCGGY | 1 cut(s) | 616 |
| Cfr13I | GGNCC | 3 cut(s) | 249, 442, 496 |
| Cfr42I | CCGCGG | 1 cut(s) | 357 |
| CseI | GACGC | 2 cut(s) | 125, 283 |
| Csp6I | GTAC | 2 cut(s) | 29, 478 |
| CviAII | CATG | 3 cut(s) | 14, 125, 198 |
| CviQI | GTAC | 2 cut(s) | 29, 478 |
| DdeI | CTNAG | 1 cut(s) | 309 |
| DpnI | GATC | 3 cut(s) | 96, 107, 324 |
| DpnII | GATC | 3 cut(s) | 94, 105, 322 |
| EaeI | YGGCCR | 2 cut(s) | 357, 510 |
| Eam1104I | CTCTTC | 3 cut(s) | 197, 220, 237 |
| EarI | CTCTTC | 3 cut(s) | 197, 220, 237 |
| EciI | GGCGGA | 1 cut(s) | 455 |
| Eco24I | GRGCYC | 1 cut(s) | 91 |
| Eco47I | GGWCC | 2 cut(s) | 249, 442 |
| Eco57I | CTGAAG | 2 cut(s) | 191, 560 |
| Eco88I | CYCGRG | 1 cut(s) | 84 |
| EcoNI | CCTNNNNNAGG | 2 cut(s) | 228, 449 |
| EcoRII | CCWGG | 1 cut(s) | 23 |
| EcoT38I | GRGCYC | 1 cut(s) | 91 |
| FaeI | CATG | 3 cut(s) | 17, 128, 201 |
| FaiI | YATR | 8 cut(s) | 15, 126, 177, 199, 227, 422, 424, 426 |
| FaqI | GGGAC | 1 cut(s) | 168 |
| FatI | CATG | 3 cut(s) | 13, 124, 197 |
| FauI | CCCGC | 2 cut(s) | 46, 361 |
| Fnu4HI | GCNGC | 3 cut(s) | 210, 357, 417 |
| FokI | GGATG | 1 cut(s) | 327 |
| FriOI | GRGCYC | 1 cut(s) | 91 |
| Fsp4HI | GCNGC | 3 cut(s) | 210, 357, 417 |
| FspBI | CTAG | 2 cut(s) | 42, 597 |
| GlaI | GCGC | 2 cut(s) | 129, 549 |
| GluI | GCNGC | 3 cut(s) | 210, 357, 417 |
| GsaI | CCCAGC | 2 cut(s) | 166, 374 |
| GsuI | CTGGAG | 2 cut(s) | 139, 572 |
| HaeII | RGCGCY | 1 cut(s) | 551 |
| HaeIII | GGCC | 3 cut(s) | 359, 497, 512 |
| HapII | CCGG | 2 cut(s) | 92, 617 |
| HgaI | GACGC | 2 cut(s) | 125, 283 |
| HhaI | GCGC | 2 cut(s) | 130, 550 |
| Hin1I | GRCGYC | 1 cut(s) | 275 |
| Hin1II | CATG | 3 cut(s) | 17, 128, 201 |
| Hin6I | GCGC | 2 cut(s) | 128, 548 |
| HinP1I | GCGC | 2 cut(s) | 128, 548 |
| HincII | GTYRAC | 1 cut(s) | 385 |
| HindII | GTYRAC | 1 cut(s) | 385 |
| HpaI | GTTAAC | 1 cut(s) | 385 |
| HpaII | CCGG | 2 cut(s) | 92, 617 |
| HphI | GGTGA | 2 cut(s) | 89, 100 |
| Hpy166II | GTNNAC | 1 cut(s) | 385 |
| Hpy188I | TCNGA | 3 cut(s) | 149, 239, 273 |
| Hpy188III | TCNNGA | 3 cut(s) | 137, 156, 407 |
| Hpy8I | GTNNAC | 1 cut(s) | 385 |
| Hpy99I | CGWCG | 2 cut(s) | 119, 536 |
| HpyAV | CCTTC | 2 cut(s) | 481, 538 |
| HpyCH4III | ACNGT | 1 cut(s) | 389 |
| HpyCH4IV | ACGT | 1 cut(s) | 601 |
| HpyCH4V | TGCA | 1 cut(s) | 392 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 74, 125, 159, 554, 580 |
| HpyF3I | CTNAG | 1 cut(s) | 309 |
| HpySE526I | ACGT | 1 cut(s) | 601 |
| Hsp92I | GRCGYC | 1 cut(s) | 275 |
| Hsp92II | CATG | 3 cut(s) | 17, 128, 201 |
| HspAI | GCGC | 2 cut(s) | 128, 548 |
| KpnI | GGTACC | 1 cut(s) | 32 |
| KspAI | GTTAAC | 1 cut(s) | 385 |
| KspI | CCGCGG | 1 cut(s) | 357 |
| Kzo9I | GATC | 3 cut(s) | 94, 105, 322 |
| LmnI | GCTCC | 3 cut(s) | 124, 158, 313 |
| Lsp1109I | GCAGC | 1 cut(s) | 221 |
| LweI | GCATC | 1 cut(s) | 243 |
| MaeI | CTAG | 2 cut(s) | 42, 597 |
| MaeII | ACGT | 1 cut(s) | 601 |
| MaeIII | GTNAC | 1 cut(s) | 49 |
| MalI | GATC | 3 cut(s) | 96, 107, 324 |
| MboI | GATC | 3 cut(s) | 94, 105, 322 |
| MboII | GAAGA | 4 cut(s) | 207, 214, 254, 553 |
| MhlI | GDGCHC | 1 cut(s) | 91 |
| MlsI | TGGCCA | 1 cut(s) | 512 |
| MluCI | AATT | 2 cut(s) | 286, 527 |
| MluNI | TGGCCA | 1 cut(s) | 512 |
| MnlI | CCTC | 8 cut(s) | 223, 240, 279, 304, 347, 455, 487, 547 |
| Mox20I | TGGCCA | 1 cut(s) | 512 |
| MscI | TGGCCA | 1 cut(s) | 512 |
| MseI | TTAA | 3 cut(s) | 366, 378, 384 |
| MslI | CAYNNNNRTG | 3 cut(s) | 12, 138, 518 |
| Msp20I | TGGCCA | 1 cut(s) | 512 |
| MspA1I | CMGCKG | 2 cut(s) | 356, 374 |
| MspI | CCGG | 2 cut(s) | 92, 617 |
| MspR9I | CCNGG | 2 cut(s) | 25, 92 |
| MvaI | CCWGG | 1 cut(s) | 25 |
| MvnI | CGCG | 3 cut(s) | 261, 356, 589 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 74, 125, 159, 554, 580 |
| NciI | CCSGG | 1 cut(s) | 92 |
| NdeII | GATC | 3 cut(s) | 94, 105, 322 |
| NheI | GCTAGC | 1 cut(s) | 41 |
| NlaIII | CATG | 3 cut(s) | 17, 128, 201 |
| NlaIV | GGNNCC | 1 cut(s) | 30 |
| NmuCI | GTSAC | 1 cut(s) | 49 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 162 |
| PkrI | GCNGC | 3 cut(s) | 211, 358, 418 |
| Psp6I | CCWGG | 1 cut(s) | 23 |
| PspFI | CCCAGC | 2 cut(s) | 162, 370 |
| PspGI | CCWGG | 1 cut(s) | 23 |
| PspN4I | GGNNCC | 1 cut(s) | 30 |
| PspPI | GGNCC | 3 cut(s) | 249, 442, 496 |
| PstNI | CAGNNNCTG | 1 cut(s) | 162 |
| PvuII | CAGCTG | 1 cut(s) | 374 |
| RsaI | GTAC | 2 cut(s) | 30, 479 |
| RsaNI | GTAC | 2 cut(s) | 29, 478 |
| RseI | CAYNNNNRTG | 3 cut(s) | 12, 138, 518 |
| SacII | CCGCGG | 1 cut(s) | 357 |
| SaqAI | TTAA | 3 cut(s) | 366, 378, 384 |
| SatI | GCNGC | 3 cut(s) | 210, 357, 417 |
| Sau3AI | GATC | 3 cut(s) | 94, 105, 322 |
| Sau96I | GGNCC | 3 cut(s) | 249, 442, 496 |
| ScrFI | CCNGG | 2 cut(s) | 25, 92 |
| SduI | GDGCHC | 1 cut(s) | 91 |
| SfaNI | GCATC | 1 cut(s) | 243 |
| Sfr303I | CCGCGG | 1 cut(s) | 357 |
| SgrBI | CCGCGG | 1 cut(s) | 357 |
| SinI | GGWCC | 2 cut(s) | 249, 442 |
| SmiMI | CAYNNNNRTG | 3 cut(s) | 12, 138, 518 |
| Sse9I | AATT | 2 cut(s) | 286, 527 |
| SspMI | CTAG | 2 cut(s) | 42, 597 |
| StyD4I | CCNGG | 2 cut(s) | 23, 90 |
| TaaI | ACNGT | 1 cut(s) | 389 |
| TaiI | ACGT | 1 cut(s) | 604 |
| TaqI | TCGA | 1 cut(s) | 531 |
| TasI | AATT | 2 cut(s) | 286, 527 |
| TatI | WGTACW | 1 cut(s) | 477 |
| TauI | GCSGC | 2 cut(s) | 359, 419 |
| Tru1I | TTAA | 3 cut(s) | 366, 378, 384 |
| Tru9I | TTAA | 3 cut(s) | 366, 378, 384 |
| TscAI | CASTG | 1 cut(s) | 441 |
| TseFI | GTSAC | 1 cut(s) | 49 |
| TseI | GCWGC | 1 cut(s) | 209 |
| Tsp45I | GTSAC | 1 cut(s) | 49 |
| TspGWI | ACGGA | 1 cut(s) | 72 |
| TspRI | CASTG | 1 cut(s) | 441 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 162 |
| VpaK11BI | GGWCC | 2 cut(s) | 249, 442 |
| XagI | CCTNNNNNAGG | 2 cut(s) | 228, 449 |
| XapI | RAATTY | 1 cut(s) | 527 |
| XspI | CTAG | 2 cut(s) | 42, 597 |